JAK1 gene cDNA ORF clone, Homo sapiens(Human)

mRNA	Protein	Name
NM_002227.2	NP_002218.2	tyrosine-protein kinase JAK1
NM_001321855.1	NP_001308784.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321853.1	NP_001308782.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321856.1	NP_001308785.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321855.2	NP_001308784.1	tyrosine-protein kinase JAK1 isoform 1
XM_006710624.1	XP_006710687.1	tyrosine-protein kinase JAK1 isoform X1
XM_017001212.1	XP_016856701.1	tyrosine-protein kinase JAK1 isoform X1
NM_001320923.1	NP_001307852.1	tyrosine-protein kinase JAK1 isoform 1
NM_002227.4	NP_002218.2	tyrosine-protein kinase JAK1 isoform 1
NM_001321853.2	NP_001308782.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321857.2	NP_001308786.1	tyrosine-protein kinase JAK1 isoform 2
NM_001321852.2	NP_001308781.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321857.1	NP_001308786.1	tyrosine-protein kinase JAK1 isoform 2
NM_001321854.1	NP_001308783.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321854.2	NP_001308783.1	tyrosine-protein kinase JAK1 isoform 1
NM_001321852.1	NP_001308781.1	tyrosine-protein kinase JAK1 isoform 1
NM_002227.3	NP_002218.2	tyrosine-protein kinase JAK1 isoform 1
XM_005270841.1	XP_005270898.1	tyrosine-protein kinase JAK1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19227	NM_001321852.2 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321853.2 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321854.2 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_002227.4 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321855.2 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321856.1 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001320923.1 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	XM_005270841.1 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	XM_006710624.1 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu58539	NM_001321857.2 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu58539	XM_017001212.1 Latest version!	Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu19227	NM_001321855.1	Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_002227.2	Homo sapiens Janus kinase 1 (JAK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321853.1	Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu58539	NM_001321857.1	Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu19227	NM_001321854.1	Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_001321852.1	Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu19227	NM_002227.3	Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

JAK1 ( NM_002227.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002218.2 Homo sapiens Janus kinase 1 (JAK1), mRNA.

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_002227.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	tyrosine-protein kinase JAK1
Comment

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_002218.2

Translation

MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_002227.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens Janus kinase 1 (JAK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002227.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321855.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308784.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321855.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14 and AC093427.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.201105.1, SRR1803611.25630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308784.1
CDS	399..3863
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321855.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321855.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321853.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308782.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321853.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35, AL606517.14 and AC093427.2. Transcript Variant: This variant (4) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.188475.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308782.1
CDS	480..3944
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321853.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321853.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321856.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308785.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 7, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14 and AC093427.2. On Apr 1, 2016 this sequence version replaced XM_005270841.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.121310.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308785.1
CDS	134..3598
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321856.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321856.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321855.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308784.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14 and AC093427.2. On May 31, 2019 this sequence version replaced NM_001321855.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.18127.1, SRR1803615.170394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308784.1
CDS	379..3843
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321855.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321855.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001320923.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001307852.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 2, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001320923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314257.1, BC062431.1, BC132729.1 and AC093427.2. On Mar 9, 2016 this sequence version replaced XM_006710624.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307852.1
CDS	218..3682
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001320923.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320923.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_002227.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002218.2 Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_002227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14, BC009079.1, AB209057.1 and AC093427.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002227.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.39138.1, SRR1803611.240972.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342505.5/ ENSP00000343204.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002218.2
CDS	295..3759
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_002227.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002227.4

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321853.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308782.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35, AL606517.14 and AC093427.2. On Jun 1, 2019 this sequence version replaced NM_001321853.1. Transcript Variant: This variant (4) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.188475.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308782.1
CDS	480..3944
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321853.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321853.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321852.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308781.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35 and AC093427.2. On May 31, 2019 this sequence version replaced NM_001321852.1. Transcript Variant: This variant (3) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.268382.1, SRR1660805.64270.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308781.1
CDS	221..3685
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321852.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321854.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308783.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321854.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35 and AC093427.2. Transcript Variant: This variant (5) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.212661.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308783.1
CDS	396..3860
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321854.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321854.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308783.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35 and AC093427.2. On May 31, 2019 this sequence version replaced NM_001321854.1. Transcript Variant: This variant (5) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.212661.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308783.1
CDS	396..3860
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321854.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321852.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308781.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_001321852.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357078.35 and AC093427.2. Transcript Variant: This variant (3) initiates from an upstream promoter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.64270.1, SRR1660807.84819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308781.1
CDS	221..3685
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321852.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321852.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_002227.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002218.2 Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.

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CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	NM_002227.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14, BC009079.1, AB209057.1 and AC093427.2. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2016 this sequence version replaced NM_002227.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002218.2
CDS	315..3779
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_002227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002227.3

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( XM_006710624.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_006710687.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	XM_006710624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	tyrosine-protein kinase JAK1 isoform X1
Comment

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006710687.1

Translation

MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	XM_006710624.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710624.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( XM_005270841.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005270898.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu19227
Clone ID Related Accession (Same CDS sequence)	NM_002227.2 , NM_001321855.1 , NM_001321853.1 , NM_001321856.1 , NM_001321855.2 , XM_006710624.1 , NM_001320923.1 , NM_002227.4 , NM_001321853.2 , NM_001321852.2 , NM_001321854.1 , NM_001321854.2 , NM_001321852.1 , NM_002227.3 , XM_005270841.1
Accession Version	XM_005270841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	tyrosine-protein kinase JAK1 isoform X1
Comment

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005270898.1

Translation

MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	XM_005270841.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270841.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGCA GGTGCAGGGT GCCCAGAAGC AGTTCAAGAA CTTTCAGATC 
GAGGTGCAGA AGGGCCGCTA CAGTCTGCAC GGTTCGGACC GCAGCTTCCC CAGCTTGGGA 
GACCTCATGA GCCACCTCAA GAAGCAGATC CTGCGCACGG ATAACATCAG CTTCATGCTA 
AAACGCTGCT GCCAGCCCAA GCCCCGAGAA ATCTCCAACC TGCTGGTGGC TACTAAGAAA 
GCCCAGGAGT GGCAGCCCGT CTACCCCATG AGCCAGCTGA GTTTCGATCG GATCCTCAAG 
AAGGATCTGG TGCAGGGCGA GCACCTTGGG AGAGGCACGA GAACACACAT CTATTCTGGG 
ACCCTGATGG ATTACAAGGA TGACGAAGGA ACTTCTGAAG AGAAGAAGAT AAAAGTGATC 
CTCAAAGTCT TAGACCCCAG CCACAGGGAT ATTTCCCTGG CCTTCTTCGA GGCAGCCAGC 
ATGATGAGAC AGGTCTCCCA CAAACACATC GTGTACCTCT ATGGCGTCTG TGTCCGCGAC 
GTGGAGAATA TCATGGTGGA AGAGTTTGTG GAAGGGGGTC CTCTGGATCT CTTCATGCAC 
CGGAAAAGCG ATGTCCTTAC CACACCATGG AAATTCAAAG TTGCCAAACA GCTGGCCAGT 
GCCCTGAGCT ACTTGGAGGA TAAAGACCTG GTCCATGGAA ATGTGTGTAC TAAAAACCTC 
CTCCTGGCCC GTGAGGGCAT CGACAGTGAG TGTGGCCCAT TCATCAAGCT CAGTGACCCC 
GGCATCCCCA TTACGGTGCT GTCTAGGCAA GAATGCATTG AACGAATCCC ATGGATTGCT 
CCTGAGTGTG TTGAGGACTC CAAGAACCTG AGTGTGGCTG CTGACAAGTG GAGCTTTGGA 
ACCACGCTCT GGGAAATCTG CTACAATGGC GAGATCCCCT TGAAAGACAA GACGCTGATT 
GAGAAAGAGA GATTCTATGA AAGCCGGTGC AGGCCAGTGA CACCATCATG TAAGGAGCTG 
GCTGACCTCA TGACCCGCTG CATGAACTAT GACCCCAATC AGAGGCCTTT CTTCCGAGCC 
ATCATGAGAG ACATTAATAA GCTTGAAGAG CAGAATCCAG ATATTGTTTC AGAAAAAAAA 
CCAGCAACTG AAGTGGACCC CACACATTTT GAAAAGCGCT TCCTAAAGAG GATCCGTGAC 
TTGGGAGAGG GCCACTTTGG GAAGGTTGAG CTCTGCAGGT ATGACCCCGA AGGGGACAAT 
ACAGGGGAGC AGGTGGCTGT TAAATCTCTG AAGCCTGAGA GTGGAGGTAA CCACATAGCT 
GATCTGAAAA AGGAAATCGA GATCTTAAGG AACCTCTATC ATGAGAACAT TGTGAAGTAC 
AAAGGAATCT GCACAGAAGA CGGAGGAAAT GGTATTAAGC TCATCATGGA ATTTCTGCCT 
TCGGGAAGCC TTAAGGAATA TCTTCCAAAG AATAAGAACA AAATAAACCT CAAACAGCAG 
CTAAAATATG CCGTTCAGAT TTGTAAGGGG ATGGACTATT TGGGTTCTCG GCAATACGTT 
CACCGGGACT TGGCAGCAAG AAATGTCCTT GTTGAGAGTG AACACCAAGT GAAAATTGGA 
GACTTCGGTT TAACCAAAGC AATTGAAACC GATAAGGAGT ATTACACCGT CAAGGATGAC 
CGGGACAGCC CTGTGTTTTG GTATGCTCCA GAATGTTTAA TGCAATCTAA ATTTTATATT 
GCCTCTGACG TCTGGTCTTT TGGAGTCACT CTGCATGAGC TGCTGACTTA CTGTGATTCA 
GATTCTAGTC CCATGGCTTT GTTCCTGAAA ATGATAGGCC CAACCCATGG CCAGATGACA 
GTCACAAGAC TTGTGAATAC GTTAAAAGAA GGAAAACGCC TGCCGTGCCC ACCTAACTGT 
CCAGATGAGG TTTATCAACT TATGAGGAAA TGCTGGGAAT TCCAACCATC CAATCGGACA 
AGCTTTCAGA ACCTTATTGA AGGATTTGAA GCACTTTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321857.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308786.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.

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CloneID	OHu58539
Clone ID Related Accession (Same CDS sequence)	XM_017001212.1 , NM_001321857.2 , NM_001321857.1
Accession Version	NM_001321857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14 and AC093427.2. On May 31, 2019 this sequence version replaced NM_001321857.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.26220.1, SRR1803617.144575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308786.1
CDS	295..3756
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321857.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321857.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( NM_001321857.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001308786.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.

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CloneID	OHu58539
Clone ID Related Accession (Same CDS sequence)	XM_017001212.1 , NM_001321857.2 , NM_001321857.1
Accession Version	NM_001321857.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606517.14 and AC093427.2. On Apr 2, 2016 this sequence version replaced XM_011541395.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.84957.1, SRR1660805.77140.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308786.1
CDS	315..3776
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	NM_001321857.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321857.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( XM_017001212.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016856701.1 Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.

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CloneID	OHu58539
Clone ID Related Accession (Same CDS sequence)	XM_017001212.1 , NM_001321857.2 , NM_001321857.1
Accession Version	XM_017001212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase JAK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856701.1
CDS	162..3623
Translation	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Target ORF information:

RefSeq Version	XM_017001212.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Janus kinase 1 (JAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001212.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA AGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGACTGAGGT GAACCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGATCTTC 
TATCTGTCGG ACAGGGAGCC CCTCCGGCTG GGCAGTGGAG AGTACACAGC AGAGGAACTG 
TGCATCAGGG CTGCACAGGC ATGCCGTATC TCTCCTCTTT GTCACAACCT CTTTGCCCTG 
TATGACGAGA ACACCAAGCT CTGGTATGCT CCAAATCGCA CCATCACCGT TGATGACAAG 
ATGTCCCTCC GGCTCCACTA CCGGATGAGG TTCTATTTCA CCAATTGGCA TGGAACCAAC 
GACAATGAGC AGTCAGTGTG GCGTCATTCT CCAAAGAAGC AGAAAAATGG CTACGAGAAA 
AAAAAGATTC CAGATGCAAC CCCTCTCCTT GATGCCAGCT CACTGGAGTA TCTGTTTGCT 
CAGGGACAGT ATGATTTGGT GAAATGCCTG GCTCCTATTC GAGACCCCAA GACCGAGCAG 
GATGGACATG ATATTGAGAA CGAGTGTCTA GGGATGGCTG TCCTGGCCAT CTCACACTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTGCCCAAGG ACATCAGCTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA GTCCATCAGA CAGAGGAACC TTCTCACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATTTG TGACAGCAGC 
GTGTCCACGC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TACGGTGCTG AAATATTTGA GACTTCCATG TTACTGATTT CATCAGAAAA TGAGATGAAT 
TGGTTTCATT CGAATGACGG TGGAAACGTT CTCTACTACG AAGTGATGGT GACTGGGAAT 
CTTGGAATCC AGTGGAGGCA TAAACCAAAT GTTGTTTCTG TTGAAAAGGA AAAAAATAAA 
CTGAAGCGGA AAAAACTGGA AAATAAACAC AAGAAGGATG AGGAGAAAAA CAAGATCCGG 
GAAGAGTGGA ACAATTTTTC TTACTTCCCT GAAATCACTC ACATTGTAAT AAAGGAGTCT 
GTGGTCAGCA TTAACAAGCA GGACAACAAG AAAATGGAAC TGAAGCTCTC TTCCCACGAG 
GAGGCCTTGT CCTTTGTGTC CCTGGTAGAT GGCTACTTCC GGCTCACAGC AGATGCCCAT 
CATTACCTCT GCACCGACGT GGCCCCCCCG TTGATCGTCC ACAACATACA GAATGGCTGT 
CATGGTCCAA TCTGTACAGA ATACGCCATC AATAAATTGC GGCAAGAAGG AAGCGAGGAG 
GGGATGTACG TGCTGAGGTG GAGCTGCACC GACTTTGACA ACATCCTCAT GACCGTCACC 
TGCTTTGAGA AGTCTGAGGT GCAGGGTGCC CAGAAGCAGT TCAAGAACTT TCAGATCGAG 
GTGCAGAAGG GCCGCTACAG TCTGCACGGT TCGGACCGCA GCTTCCCCAG CTTGGGAGAC 
CTCATGAGCC ACCTCAAGAA GCAGATCCTG CGCACGGATA ACATCAGCTT CATGCTAAAA 
CGCTGCTGCC AGCCCAAGCC CCGAGAAATC TCCAACCTGC TGGTGGCTAC TAAGAAAGCC 
CAGGAGTGGC AGCCCGTCTA CCCCATGAGC CAGCTGAGTT TCGATCGGAT CCTCAAGAAG 
GATCTGGTGC AGGGCGAGCA CCTTGGGAGA GGCACGAGAA CACACATCTA TTCTGGGACC 
CTGATGGATT ACAAGGATGA CGAAGGAACT TCTGAAGAGA AGAAGATAAA AGTGATCCTC 
AAAGTCTTAG ACCCCAGCCA CAGGGATATT TCCCTGGCCT TCTTCGAGGC AGCCAGCATG 
ATGAGACAGG TCTCCCACAA ACACATCGTG TACCTCTATG GCGTCTGTGT CCGCGACGTG 
GAGAATATCA TGGTGGAAGA GTTTGTGGAA GGGGGTCCTC TGGATCTCTT CATGCACCGG 
AAAAGCGATG TCCTTACCAC ACCATGGAAA TTCAAAGTTG CCAAACAGCT GGCCAGTGCC 
CTGAGCTACT TGGAGGATAA AGACCTGGTC CATGGAAATG TGTGTACTAA AAACCTCCTC 
CTGGCCCGTG AGGGCATCGA CAGTGAGTGT GGCCCATTCA TCAAGCTCAG TGACCCCGGC 
ATCCCCATTA CGGTGCTGTC TAGGCAAGAA TGCATTGAAC GAATCCCATG GATTGCTCCT 
GAGTGTGTTG AGGACTCCAA GAACCTGAGT GTGGCTGCTG ACAAGTGGAG CTTTGGAACC 
ACGCTCTGGG AAATCTGCTA CAATGGCGAG ATCCCCTTGA AAGACAAGAC GCTGATTGAG 
AAAGAGAGAT TCTATGAAAG CCGGTGCAGG CCAGTGACAC CATCATGTAA GGAGCTGGCT 
GACCTCATGA CCCGCTGCAT GAACTATGAC CCCAATCAGA GGCCTTTCTT CCGAGCCATC 
ATGAGAGACA TTAATAAGCT TGAAGAGCAG AATCCAGATA TTGTTTCAGA AAAAAAACCA 
GCAACTGAAG TGGACCCCAC ACATTTTGAA AAGCGCTTCC TAAAGAGGAT CCGTGACTTG 
GGAGAGGGCC ACTTTGGGAA GGTTGAGCTC TGCAGGTATG ACCCCGAAGG GGACAATACA 
GGGGAGCAGG TGGCTGTTAA ATCTCTGAAG CCTGAGAGTG GAGGTAACCA CATAGCTGAT 
CTGAAAAAGG AAATCGAGAT CTTAAGGAAC CTCTATCATG AGAACATTGT GAAGTACAAA 
GGAATCTGCA CAGAAGACGG AGGAAATGGT ATTAAGCTCA TCATGGAATT TCTGCCTTCG 
GGAAGCCTTA AGGAATATCT TCCAAAGAAT AAGAACAAAA TAAACCTCAA ACAGCAGCTA 
AAATATGCCG TTCAGATTTG TAAGGGGATG GACTATTTGG GTTCTCGGCA ATACGTTCAC 
CGGGACTTGG CAGCAAGAAA TGTCCTTGTT GAGAGTGAAC ACCAAGTGAA AATTGGAGAC 
TTCGGTTTAA CCAAAGCAAT TGAAACCGAT AAGGAGTATT ACACCGTCAA GGATGACCGG 
GACAGCCCTG TGTTTTGGTA TGCTCCAGAA TGTTTAATGC AATCTAAATT TTATATTGCC 
TCTGACGTCT GGTCTTTTGG AGTCACTCTG CATGAGCTGC TGACTTACTG TGATTCAGAT 
TCTAGTCCCA TGGCTTTGTT CCTGAAAATG ATAGGCCCAA CCCATGGCCA GATGACAGTC 
ACAAGACTTG TGAATACGTT AAAAGAAGGA AAACGCCTGC CGTGCCCACC TAACTGTCCA 
GATGAGGTTT ATCAACTTAT GAGGAAATGC TGGGAATTCC AACCATCCAA TCGGACAAGC 
TTTCAGAACC TTATTGAAGG ATTTGAAGCA CTTTTAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	JAK1
Entrez Gene ID	3716
Full Name	Janus kinase 1
Synonyms	JAK1A,JAK1B,JTK3
General protein information	Preferred Names Janus kinase 1 Names tyrosine-protein kinase JAK1
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 1 Map Location: 1p31.3
Summary	This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016].
Disorder MIM:	147795

Xenopus tropicalis (tropical clawed frog)	jak1	XP_002931642.1
Gallus gallus (chicken)	JAK1	NP_990201.1
Danio rerio (zebrafish)	jak1	NP_571148.1
Homo sapiens (human)	JAK1	NP_002218.2
	JAK1	XP_002801666.1
Canis lupus familiaris (dog)	JAK1	XP_536679.2
Rattus norvegicus (Norway rat)	Jak1	NP_445918.1
Pan troglodytes (chimpanzee)	JAK1	XP_001161242.1
Bos taurus (cattle)	JAK1	NP_001193463.1
Mus musculus (house mouse)	Jak1	NP_666257.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

JAK1 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

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