IDE gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001322796.1	NP_001309725.1	insulin-degrading enzyme isoform 5
NM_001165946.1	NP_001159418.1	insulin-degrading enzyme isoform 2
NM_001322797.1	NP_001309726.1	insulin-degrading enzyme isoform 6
XM_017016188.1	XP_016871677.1	insulin-degrading enzyme isoform X1
XM_017016190.1	XP_016871679.1	insulin-degrading enzyme isoform X2
NM_004969.4	NP_004960.2	insulin-degrading enzyme isoform 1
NM_001322795.1	NP_001309724.1	insulin-degrading enzyme isoform 5
XM_017016189.1	XP_016871678.1	insulin-degrading enzyme isoform X1
NM_004969.3	NP_004960.2	insulin-degrading enzyme isoform 1
NM_001322794.1	NP_001309723.1	insulin-degrading enzyme isoform 4
NM_001322793.1	NP_001309722.1	insulin-degrading enzyme isoform 3
XM_017016187.1	XP_016871676.1	insulin-degrading enzyme isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26132	NM_001165946.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26242	NM_004969.4 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu100585	NM_001322797.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu91378	NM_001322795.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu91378	NM_001322796.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu91378	XM_017016188.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu91378	XM_017016189.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu91378	XM_017016187.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu100620	NM_001322794.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu91379	XM_017016190.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu44273	NM_001322793.1 Latest version!	Homo sapiens insulin degrading enzyme (IDE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu26242	NM_004969.3	Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

IDE ( NM_001165946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159418.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26132
Clone ID Related Accession (Same CDS sequence)	NM_001165946.1
Accession Version	NM_001165946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB445060.1, AK316407.1, BX648462.1, CK824565.1, DA879845.1 and CF780766.1. Transcript Variant: This variant (2) lacks multiple exons that are present in the 5' end of variant 1. Variant 2 contains a novel exon at its 5' end and begins translation from a downstream in-frame start codon, compared to variant 1. The encoded protein (isoform 2) lacks 555 amino acids from the N-terminus of isoform 1 but is identical to its C-terminal 464 amino acids. Compared to isoform 1, isoform 2 lacks two of three M16 peptidase domains and the N-terminal catalytic domain. The C-terminus of isoform 2 retains the oligomerization domain and peroxisomal targeting sequence present in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302578.1, AK316407.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGCAAAC TTTGGTTCAA ACAAGATGAT AAGTTTTTTT TGCCGAAGGC TTGTCTCAAC 
TTTGAATTTT TCAGCCCATT TGCTTATGTG GACCCCTTGC ACTGTAACAT GGCCTATTTG 
TACCTTGAGC TCCTCAAAGA CTCACTCAAC GAGTATGCAT ATGCAGCAGA GCTAGCAGGC 
TTGAGCTATG ATCTCCAAAA TACCATCTAT GGGATGTATC TTTCAGTGAA AGGTTACAAT 
GACAAGCAGC CAATTTTACT AAAGAAGATT ATTGAGAAAA TGGCTACCTT TGAGATTGAT 
GAAAAAAGAT TTGAAATTAT CAAAGAAGCA TATATGCGAT CTCTTAACAA TTTCCGGGCT 
GAACAGCCTC ACCAGCATGC CATGTACTAC CTCCGCTTGC TGATGACTGA AGTGGCCTGG 
ACTAAAGATG AGTTAAAAGA AGCTCTGGAT GATGTAACCC TTCCTCGCCT TAAGGCCTTC 
ATACCTCAGC TCCTGTCACG GCTGCACATT GAAGCCCTTC TCCATGGAAA CATAACAAAG 
CAGGCTGCAT TAGGAATTAT GCAGATGGTT GAAGACACCC TCATTGAACA TGCTCATACC 
AAACCTCTCC TTCCAAGTCA GCTGGTTCGG TATAGAGAAG TTCAGCTCCC TGACAGAGGA 
TGGTTTGTTT ATCAGCAGAG AAATGAAGTT CACAATAACT GTGGCATCGA GATATACTAC 
CAAACAGACA TGCAAAGCAC CTCAGAGAAT ATGTTTCTGG AGCTCTTCTG TCAGATTATC 
TCGGAACCTT GCTTCAACAC CCTGCGCACC AAGGAGCAGT TGGGCTATAT CGTCTTCAGC 
GGGCCACGTC GAGCTAATGG CATACAGGGC TTGAGATTCA TCATCCAGTC AGAAAAGCCA 
CCTCACTACC TAGAAAGCAG AGTGGAAGCT TTCTTAATTA CCATGGAAAA GTCCATAGAG 
GACATGACAG AAGAGGCCTT CCAAAAACAC ATTCAGGCAT TAGCAATTCG TCGACTAGAC 
AAACCAAAGA AGCTATCTGC TGAGTGTGCT AAATACTGGG GAGAAATCAT CTCCCAGCAA 
TATAATTTTG ACAGAGATAA CACTGAGGTT GCATATTTAA AGACACTTAC CAAGGAAGAT 
ATCATCAAAT TCTACAAGGA AATGTTGGCA GTAGATGCTC CAAGGAGACA TAAGGTATCC 
GTCCATGTTC TTGCCAGGGA AATGGATTCT TGTCCTGTTG TTGGAGAGTT CCCATGTCAA 
AATGACATAA ATTTGTCACA AGCACCAGCC TTGCCACAAC CTGAAGTGAT TCAGAACATG 
ACCGAATTCA AGCGTGGTCT GCCACTGTTT CCCCTTGTGA AACCACATAT TAACTTCATG 
GCTGCAAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159418.1
CDS	326..1720
Translation	MSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001165946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165946.1

ORF Insert Sequence:

ATGAGCAAAC TTTGGTTCAA ACAAGATGAT AAGTTTTTTT TGCCGAAGGC TTGTCTCAAC 
TTTGAATTTT TCAGCCCATT TGCTTATGTG GACCCCTTGC ACTGTAACAT GGCCTATTTG 
TACCTTGAGC TCCTCAAAGA CTCACTCAAC GAGTATGCAT ATGCAGCAGA GCTAGCAGGC 
TTGAGCTATG ATCTCCAAAA TACCATCTAT GGGATGTATC TTTCAGTGAA AGGTTACAAT 
GACAAGCAGC CAATTTTACT AAAGAAGATT ATTGAGAAAA TGGCTACCTT TGAGATTGAT 
GAAAAAAGAT TTGAAATTAT CAAAGAAGCA TATATGCGAT CTCTTAACAA TTTCCGGGCT 
GAACAGCCTC ACCAGCATGC CATGTACTAC CTCCGCTTGC TGATGACTGA AGTGGCCTGG 
ACTAAAGATG AGTTAAAAGA AGCTCTGGAT GATGTAACCC TTCCTCGCCT TAAGGCCTTC 
ATACCTCAGC TCCTGTCACG GCTGCACATT GAAGCCCTTC TCCATGGAAA CATAACAAAG 
CAGGCTGCAT TAGGAATTAT GCAGATGGTT GAAGACACCC TCATTGAACA TGCTCATACC 
AAACCTCTCC TTCCAAGTCA GCTGGTTCGG TATAGAGAAG TTCAGCTCCC TGACAGAGGA 
TGGTTTGTTT ATCAGCAGAG AAATGAAGTT CACAATAACT GTGGCATCGA GATATACTAC 
CAAACAGACA TGCAAAGCAC CTCAGAGAAT ATGTTTCTGG AGCTCTTCTG TCAGATTATC 
TCGGAACCTT GCTTCAACAC CCTGCGCACC AAGGAGCAGT TGGGCTATAT CGTCTTCAGC 
GGGCCACGTC GAGCTAATGG CATACAGGGC TTGAGATTCA TCATCCAGTC AGAAAAGCCA 
CCTCACTACC TAGAAAGCAG AGTGGAAGCT TTCTTAATTA CCATGGAAAA GTCCATAGAG 
GACATGACAG AAGAGGCCTT CCAAAAACAC ATTCAGGCAT TAGCAATTCG TCGACTAGAC 
AAACCAAAGA AGCTATCTGC TGAGTGTGCT AAATACTGGG GAGAAATCAT CTCCCAGCAA 
TATAATTTTG ACAGAGATAA CACTGAGGTT GCATATTTAA AGACACTTAC CAAGGAAGAT 
ATCATCAAAT TCTACAAGGA AATGTTGGCA GTAGATGCTC CAAGGAGACA TAAGGTATCC 
GTCCATGTTC TTGCCAGGGA AATGGATTCT TGTCCTGTTG TTGGAGAGTT CCCATGTCAA 
AATGACATAA ATTTGTCACA AGCACCAGCC TTGCCACAAC CTGAAGTGAT TCAGAACATG 
ACCGAATTCA AGCGTGGTCT GCCACTGTTT CCCCTTGTGA AACCACATAT TAACTTCATG 
GCTGCAAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_004969.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004960.2 Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26242
Clone ID Related Accession (Same CDS sequence)	NM_004969.4 , NM_004969.3
Accession Version	NM_004969.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB219527.1, BC096337.1, BX648462.1, CK824565.1, DA879845.1 and CF780766.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004969.3. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Alternative translation initiation of this transcript variant produces an isoform that lacks 41 aa from the N-terminus and, purportedly, lacks an N-terminal mitochondrial targeting sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.243749.1, SRR1803614.162726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265986.11/ ENSP00000265986.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGATCTC 
CAAAATACCA TCTATGGGAT GTATCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004960.2
CDS	75..3134
Translation	MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_004969.4
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004969.4

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGATCTC 
CAAAATACCA TCTATGGGAT GTATCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_004969.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004960.2 Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26242
Clone ID Related Accession (Same CDS sequence)	NM_004969.4 , NM_004969.3
Accession Version	NM_004969.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB219527.1, BC096337.1, BX648462.1, CK824565.1, DA879845.1 and CF780766.1. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2009 this sequence version replaced NM_004969.2. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Alternative translation initiation of this transcript variant produces an isoform that lacks 41 aa from the N-terminus and, purportedly, lacks an N-terminal mitochondrial targeting sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.198296.1, SRR1660803.42394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGATCTC 
CAAAATACCA TCTATGGGAT GTATCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004960.2
CDS	77..3136
Translation	MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_004969.3
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004969.3

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGATCTC 
CAAAATACCA TCTATGGGAT GTATCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001322797.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309726.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu100585
Clone ID Related Accession (Same CDS sequence)	NM_001322797.1
Accession Version	NM_001322797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356128.27 and AK093287.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093287.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGCAAAC TTTGGTTCAA ACAAGATGAT AAGTTTTTTT TGCCGAAGGC TTGTCTCAAC 
TTTGAATTTT TCAGTCGCTA CATTTATGCT GATCCTCTCC ATTGCAACAT GACATACCTG 
TTTATCAGGT TATTGAAGGA TGATTTAAAA GAGTATACAT ATGCAGCACG CCTCTCAGGT 
TTGAGCTATG GCATTGCATC AGGAATGAAT GCAATACTTC TTTCAGTGAA AGGTTACAAT 
GACAAGCAGC CAATTTTACT AAAGAAGATT ATTGAGAAAA TGGCTACCTT TGAGATTGAT 
GAAAAAAGAT TTGAAATTAT CAAAGAAGCA TATATGCGAT CTCTTAACAA TTTCCGGGCT 
GAACAGCCTC ACCAGCATGC CATGTACTAC CTCCGCTTGC TGATGACTGA AGTGGCCTGG 
ACTAAAGATG AGTTAAAAGA AGCTCTGGAT GATGTAACCC TTCCTCGCCT TAAGGCCTTC 
ATACCTCAGC TCCTGTCACG GCTGCACATT GAAGCCCTTC TCCATGGAAA CATAACAAAG 
CAGGCTGCAT TAGGAATTAT GCAGATGGTT GAAGACACCC TCATTGAACA TGCTCATACC 
AAACCTCTCC TTCCAAGTCA GCTGGTTCGG TATAGAGAAG TTCAGCTCCC TGACAGAGGA 
TGGTTTGTTT ATCAGCAGAG AAATGAAGTT CACAATAACT GTGGCATCGA GATATACTAC 
CAAACAGACA TGCAAAGCAC CTCAGAGAAT ATGTTTCTGG AGCTCTTCTG TCAGATTATC 
TCGGAACCTT GCTTCAACAC CCTGCGCACC AAGGAGCAGT TGGGCTATAT CGTCTTCAGC 
GGGCCACGTC GAGCTAATGG CATACAGGGC TTGAGATTCA TCATCCAGTC AGAAAAGCCA 
CCTCACTACC TAGAAAGCAG AGTGGAAGCT TTCTTAATTA CCATGGAAAA GTCCATAGAG 
GACATGACAG AAGAGGCCTT CCAAAAACAC ATTCAGGCAT TAGCAATTCG TCGACTAGAC 
AAACCAAAGA AGCTATCTGC TGAGTGTGCT AAATACTGGG GAGAAATCAT CTCCCAGCAA 
TATAATTTTG ACAGAGATAA CACTGAGGTT GCATATTTAA AGACACTTAC CAAGGAAGAT 
ATCATCAAAT TCTACAAGGA AATGTTGGCA GTAGATGCTC CAAGGAGACA TAAGGTATCC 
GTCCATGTTC TTGCCAGGGA AATGGATTCT TGTCCTGTTG TTGGAGAGTT CCCATGTCAA 
AATGACATAA ATTTGTCACA AGCACCAGCC TTGCCACAAC CTGAAGTGAT TCAGAACATG 
ACCGAATTCA AGCGTGGTCT GCCACTGTTT CCCCTTGTGA AACCACATAT TAACTTCATG 
GCTGCAAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309726.1
CDS	326..1720
Translation	MSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001322797.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322797.1

ORF Insert Sequence:

ATGAGCAAAC TTTGGTTCAA ACAAGATGAT AAGTTTTTTT TGCCGAAGGC TTGTCTCAAC 
TTTGAATTTT TCAGTCGCTA CATTTATGCT GATCCTCTCC ATTGCAACAT GACATACCTG 
TTTATCAGGT TATTGAAGGA TGATTTAAAA GAGTATACAT ATGCAGCACG CCTCTCAGGT 
TTGAGCTATG GCATTGCATC AGGAATGAAT GCAATACTTC TTTCAGTGAA AGGTTACAAT 
GACAAGCAGC CAATTTTACT AAAGAAGATT ATTGAGAAAA TGGCTACCTT TGAGATTGAT 
GAAAAAAGAT TTGAAATTAT CAAAGAAGCA TATATGCGAT CTCTTAACAA TTTCCGGGCT 
GAACAGCCTC ACCAGCATGC CATGTACTAC CTCCGCTTGC TGATGACTGA AGTGGCCTGG 
ACTAAAGATG AGTTAAAAGA AGCTCTGGAT GATGTAACCC TTCCTCGCCT TAAGGCCTTC 
ATACCTCAGC TCCTGTCACG GCTGCACATT GAAGCCCTTC TCCATGGAAA CATAACAAAG 
CAGGCTGCAT TAGGAATTAT GCAGATGGTT GAAGACACCC TCATTGAACA TGCTCATACC 
AAACCTCTCC TTCCAAGTCA GCTGGTTCGG TATAGAGAAG TTCAGCTCCC TGACAGAGGA 
TGGTTTGTTT ATCAGCAGAG AAATGAAGTT CACAATAACT GTGGCATCGA GATATACTAC 
CAAACAGACA TGCAAAGCAC CTCAGAGAAT ATGTTTCTGG AGCTCTTCTG TCAGATTATC 
TCGGAACCTT GCTTCAACAC CCTGCGCACC AAGGAGCAGT TGGGCTATAT CGTCTTCAGC 
GGGCCACGTC GAGCTAATGG CATACAGGGC TTGAGATTCA TCATCCAGTC AGAAAAGCCA 
CCTCACTACC TAGAAAGCAG AGTGGAAGCT TTCTTAATTA CCATGGAAAA GTCCATAGAG 
GACATGACAG AAGAGGCCTT CCAAAAACAC ATTCAGGCAT TAGCAATTCG TCGACTAGAC 
AAACCAAAGA AGCTATCTGC TGAGTGTGCT AAATACTGGG GAGAAATCAT CTCCCAGCAA 
TATAATTTTG ACAGAGATAA CACTGAGGTT GCATATTTAA AGACACTTAC CAAGGAAGAT 
ATCATCAAAT TCTACAAGGA AATGTTGGCA GTAGATGCTC CAAGGAGACA TAAGGTATCC 
GTCCATGTTC TTGCCAGGGA AATGGATTCT TGTCCTGTTG TTGGAGAGTT CCCATGTCAA 
AATGACATAA ATTTGTCACA AGCACCAGCC TTGCCACAAC CTGAAGTGAT TCAGAACATG 
ACCGAATTCA AGCGTGGTCT GCCACTGTTT CCCCTTGTGA AACCACATAT TAACTTCATG 
GCTGCAAAAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001322796.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309725.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu91378
Clone ID Related Accession (Same CDS sequence)	NM_001322796.1 , XM_017016188.1 , NM_001322795.1 , XM_017016189.1 , XM_017016187.1
Accession Version	NM_001322796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648462.1 and AL356128.27. Transcript Variant: This variant (6) and variant 5 both encode isoform (5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648462.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309725.1
CDS	364..3300
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001322796.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322796.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001322795.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309724.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 5, mRNA.

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CloneID	OHu91378
Clone ID Related Accession (Same CDS sequence)	NM_001322796.1 , XM_017016188.1 , NM_001322795.1 , XM_017016189.1 , XM_017016187.1
Accession Version	NM_001322795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356128.27. Transcript Variant: This variant (5) and variant 6 both encode isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.218054.1, SRR1803614.129185.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309724.1
CDS	382..3318
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001322795.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322795.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_017016188.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871677.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant X2, mRNA.

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CloneID	OHu91378
Clone ID Related Accession (Same CDS sequence)	NM_001322796.1 , XM_017016188.1 , NM_001322795.1 , XM_017016189.1 , XM_017016187.1
Accession Version	XM_017016188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871677.1
CDS	717..3653
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_017016188.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016188.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_017016189.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871678.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu91378
Clone ID Related Accession (Same CDS sequence)	NM_001322796.1 , XM_017016188.1 , NM_001322795.1 , XM_017016189.1 , XM_017016187.1
Accession Version	XM_017016189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871678.1
CDS	116..3052
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_017016189.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016189.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_017016187.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871676.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant X1, mRNA.

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CloneID	OHu91378
Clone ID Related Accession (Same CDS sequence)	NM_001322796.1 , XM_017016188.1 , NM_001322795.1 , XM_017016189.1 , XM_017016187.1
Accession Version	XM_017016187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871676.1
CDS	123..3059
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_017016187.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016187.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGATCTCCAA 
AATACCATCT ATGGGATGTA TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001322794.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309723.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 4, mRNA.

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CloneID	OHu100620
Clone ID Related Accession (Same CDS sequence)	NM_001322794.1
Accession Version	NM_001322794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356128.27. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.124882.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTCGAGAAT CTTTAGATGA CTTGACTAAT CTGGTGGTAA AGTTATTTTC TGAAGTAGAG 
AACAAAAATG TTCCATTGCC AGAATTTCCT GAACACCCTT TCCAAGAAGA ACATCTTAAA 
CAACTTTACA AAATAGTACC CATTAAAGAT ATTAGGAATC TCTATGTGAC ATTTCCCATA 
CCTGACCTTC AGAAATACTA CAAATCAAAT CCTGGTCATT ATCTTGGTCA TCTCATTGGG 
CATGAAGGTC CTGGAAGTCT GTTATCAGAA CTTAAGTCAA AGGGCTGGGT TAATACTCTT 
GTTGGTGGGC AGAAGGAAGG AGCCCGAGGT TTTATGTTTT TTATCATTAA TGTGGACTTG 
ACCGAGGAAG GATTATTACA TGTTGAAGAT ATAATTTTGC ACATGTTTCA ATACATTCAG 
AAGTTACGTG CAGAAGGACC TCAAGAATGG GTTTTCCAAG AGTGCAAGGA CTTGAATGCT 
GTTGCTTTTA GGTTTAAAGA CAAAGAGAGG CCACGGGGCT ATACATCTAA GATTGCAGGA 
ATATTGCATT ATTATCCCCT AGAAGAGGTG CTCACAGCGG AATATTTACT GGAAGAATTT 
AGACCTGACT TAATAGAGAT GGTTCTCGAT AAACTCAGAC CAGAAAATGT CCGGGTTGCC 
ATAGTTTCTA AATCTTTTGA AGGAAAAACT GATCGCACAG AAGAGTGGTA TGGAACCCAG 
TACAAACAAG AAGCTATACC GGATGAAGTC ATCAAGAAAT GGCAAAATGC TGACCTGAAT 
GGGAAATTTA AACTTCCTAC AAAGAATGAA TTTATTCCTA CGAATTTTGA GATTTTACCG 
TTAGAAAAAG AGGCGACACC ATACCCTGCT CTTATTAAGG ATACAGCTAT GAGCAAACTT 
TGGTTCAAAC AAGATGATAA GTTTTTTTTG CCGAAGGCTT GTCTCAACTT TGAATTTTTC 
AGCCCATTTG CTTATGTGGA CCCCTTGCAC TGTAACATGG CCTATTTGTA CCTTGAGCTC 
CTCAAAGACT CACTCAACGA GTATGCATAT GCAGCAGAGC TAGCAGGCTT GAGCTATGAT 
CTCCAAAATA CCATCTATGG GATGTATCTT TCAGTGAAAG GTTACAATGA CAAGCAGCCA 
ATTTTACTAA AGAAGATTAT TGAGAAAATG GCTACCTTTG AGATTGATGA AAAAAGATTT 
GAAATTATCA AAGAAGCATA TATGCGATCT CTTAACAATT TCCGGGCTGA ACAGCCTCAC 
CAGCATGCCA TGTACTACCT CCGCTTGCTG ATGACTGAAG TGGCCTGGAC TAAAGATGAG 
TTAAAAGAAG CTCTGGATGA TGTAACCCTT CCTCGCCTTA AGGCCTTCAT ACCTCAGCTC 
CTGTCACGGC TGCACATTGA AGCCCTTCTC CATGGAAACA TAACAAAGCA GGCTGCATTA 
GGAATTATGC AGATGGTTGA AGACACCCTC ATTGAACATG CTCATACCAA ACCTCTCCTT 
CCAAGTCAGC TGGTTCGGTA TAGAGAAGTT CAGCTCCCTG ACAGAGGATG GTTTGTTTAT 
CAGCAGAGAA ATGAAGTTCA CAATAACTGT GGCATCGAGA TATACTACCA AACAGACATG 
CAAAGCACCT CAGAGAATAT GTTTCTGGAG CTCTTCTGTC AGATTATCTC GGAACCTTGC 
TTCAACACCC TGCGCACCAA GGAGCAGTTG GGCTATATCG TCTTCAGCGG GCCACGTCGA 
GCTAATGGCA TACAGGGCTT GAGATTCATC ATCCAGTCAG AAAAGCCACC TCACTACCTA 
GAAAGCAGAG TGGAAGCTTT CTTAATTACC ATGGAAAAGT CCATAGAGGA CATGACAGAA 
GAGGCCTTCC AAAAACACAT TCAGGCATTA GCAATTCGTC GACTAGACAA ACCAAAGAAG 
CTATCTGCTG AGTGTGCTAA ATACTGGGGA GAAATCATCT CCCAGCAATA TAATTTTGAC 
AGAGATAACA CTGAGGTTGC ATATTTAAAG ACACTTACCA AGGAAGATAT CATCAAATTC 
TACAAGGAAA TGTTGGCAGT AGATGCTCCA AGGAGACATA AGGTATCCGT CCATGTTCTT 
GCCAGGGAAA TGGATTCTTG TCCTGTTGTT GGAGAGTTCC CATGTCAAAA TGACATAAAT 
TTGTCACAAG CACCAGCCTT GCCACAACCT GAAGTGATTC AGAACATGAC CGAATTCAAG 
CGTGGTCTGC CACTGTTTCC CCTTGTGAAA CCACATATTA ACTTCATGGC TGCAAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309723.1
CDS	77..3019
Translation	MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001322794.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322794.1

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTCGAGAAT CTTTAGATGA CTTGACTAAT CTGGTGGTAA AGTTATTTTC TGAAGTAGAG 
AACAAAAATG TTCCATTGCC AGAATTTCCT GAACACCCTT TCCAAGAAGA ACATCTTAAA 
CAACTTTACA AAATAGTACC CATTAAAGAT ATTAGGAATC TCTATGTGAC ATTTCCCATA 
CCTGACCTTC AGAAATACTA CAAATCAAAT CCTGGTCATT ATCTTGGTCA TCTCATTGGG 
CATGAAGGTC CTGGAAGTCT GTTATCAGAA CTTAAGTCAA AGGGCTGGGT TAATACTCTT 
GTTGGTGGGC AGAAGGAAGG AGCCCGAGGT TTTATGTTTT TTATCATTAA TGTGGACTTG 
ACCGAGGAAG GATTATTACA TGTTGAAGAT ATAATTTTGC ACATGTTTCA ATACATTCAG 
AAGTTACGTG CAGAAGGACC TCAAGAATGG GTTTTCCAAG AGTGCAAGGA CTTGAATGCT 
GTTGCTTTTA GGTTTAAAGA CAAAGAGAGG CCACGGGGCT ATACATCTAA GATTGCAGGA 
ATATTGCATT ATTATCCCCT AGAAGAGGTG CTCACAGCGG AATATTTACT GGAAGAATTT 
AGACCTGACT TAATAGAGAT GGTTCTCGAT AAACTCAGAC CAGAAAATGT CCGGGTTGCC 
ATAGTTTCTA AATCTTTTGA AGGAAAAACT GATCGCACAG AAGAGTGGTA TGGAACCCAG 
TACAAACAAG AAGCTATACC GGATGAAGTC ATCAAGAAAT GGCAAAATGC TGACCTGAAT 
GGGAAATTTA AACTTCCTAC AAAGAATGAA TTTATTCCTA CGAATTTTGA GATTTTACCG 
TTAGAAAAAG AGGCGACACC ATACCCTGCT CTTATTAAGG ATACAGCTAT GAGCAAACTT 
TGGTTCAAAC AAGATGATAA GTTTTTTTTG CCGAAGGCTT GTCTCAACTT TGAATTTTTC 
AGCCCATTTG CTTATGTGGA CCCCTTGCAC TGTAACATGG CCTATTTGTA CCTTGAGCTC 
CTCAAAGACT CACTCAACGA GTATGCATAT GCAGCAGAGC TAGCAGGCTT GAGCTATGAT 
CTCCAAAATA CCATCTATGG GATGTATCTT TCAGTGAAAG GTTACAATGA CAAGCAGCCA 
ATTTTACTAA AGAAGATTAT TGAGAAAATG GCTACCTTTG AGATTGATGA AAAAAGATTT 
GAAATTATCA AAGAAGCATA TATGCGATCT CTTAACAATT TCCGGGCTGA ACAGCCTCAC 
CAGCATGCCA TGTACTACCT CCGCTTGCTG ATGACTGAAG TGGCCTGGAC TAAAGATGAG 
TTAAAAGAAG CTCTGGATGA TGTAACCCTT CCTCGCCTTA AGGCCTTCAT ACCTCAGCTC 
CTGTCACGGC TGCACATTGA AGCCCTTCTC CATGGAAACA TAACAAAGCA GGCTGCATTA 
GGAATTATGC AGATGGTTGA AGACACCCTC ATTGAACATG CTCATACCAA ACCTCTCCTT 
CCAAGTCAGC TGGTTCGGTA TAGAGAAGTT CAGCTCCCTG ACAGAGGATG GTTTGTTTAT 
CAGCAGAGAA ATGAAGTTCA CAATAACTGT GGCATCGAGA TATACTACCA AACAGACATG 
CAAAGCACCT CAGAGAATAT GTTTCTGGAG CTCTTCTGTC AGATTATCTC GGAACCTTGC 
TTCAACACCC TGCGCACCAA GGAGCAGTTG GGCTATATCG TCTTCAGCGG GCCACGTCGA 
GCTAATGGCA TACAGGGCTT GAGATTCATC ATCCAGTCAG AAAAGCCACC TCACTACCTA 
GAAAGCAGAG TGGAAGCTTT CTTAATTACC ATGGAAAAGT CCATAGAGGA CATGACAGAA 
GAGGCCTTCC AAAAACACAT TCAGGCATTA GCAATTCGTC GACTAGACAA ACCAAAGAAG 
CTATCTGCTG AGTGTGCTAA ATACTGGGGA GAAATCATCT CCCAGCAATA TAATTTTGAC 
AGAGATAACA CTGAGGTTGC ATATTTAAAG ACACTTACCA AGGAAGATAT CATCAAATTC 
TACAAGGAAA TGTTGGCAGT AGATGCTCCA AGGAGACATA AGGTATCCGT CCATGTTCTT 
GCCAGGGAAA TGGATTCTTG TCCTGTTGTT GGAGAGTTCC CATGTCAAAA TGACATAAAT 
TTGTCACAAG CACCAGCCTT GCCACAACCT GAAGTGATTC AGAACATGAC CGAATTCAAG 
CGTGGTCTGC CACTGTTTCC CCTTGTGAAA CCACATATTA ACTTCATGGC TGCAAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_017016190.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871679.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91379
Clone ID Related Accession (Same CDS sequence)	XM_017016190.1
Accession Version	XM_017016190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGTCGC 
TACATTTATG CTGATCCTCT CCATTGCAAC ATGACATACC TGTTTATCAG GTTATTGAAG 
GATGATTTAA AAGAGTATAC ATATGCAGCA CGCCTCTCAG GTTTGAGCTA TGGCATTGCA 
TCAGGAATGA ATGCAATACT TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871679.1
CDS	243..3179
Translation	MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_017016190.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016190.1

ORF Insert Sequence:

ATGAATAATC CAGCCATCAA GAGAATAGGA AATCACATTA CCAAGTCTCC TGAAGACAAG 
CGAGAATATC GAGGGCTAGA GCTGGCCAAT GGTATCAAAG TACTTCTTAT CAGTGATCCC 
ACCACGGATA AGTCATCAGC AGCACTTGAT GTGCACATAG GTTCATTGTC GGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAA CATATGCTTT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAGCATG CAGGAAGTTC AAATGCCTTT 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCC 
CTAGACAGGT TTGCACAGTT TTTTCTGTGC CCCTTGTTCG ATGAAAGTTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAATTGG AAAAAGCTAC AGGGAATCCT AAACACCCCT TCAGTAAATT TGGGACAGGT 
AACAAATATA CTCTGGAGAC TAGACCAAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTA 
CTGAAATTCC ATTCTGCTTA CTATTCATCC AACTTAATGG CTGTTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTTCCAT TGCCAGAATT TCCTGAACAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA AGATATTAGG AATCTCTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCTGGT CATTATCTTG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAG TCAAAGGGCT GGGTTAATAC TCTTGTTGGT 
GGGCAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACCGAG 
GAAGGATTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGTGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGACAAAGA GAGGCCACGG GGCTATACAT CTAAGATTGC AGGAATATTG 
CATTATTATC CCCTAGAAGA GGTGCTCACA GCGGAATATT TACTGGAAGA ATTTAGACCT 
GACTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCCATAGTT 
TCTAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTATGGAAC CCAGTACAAA 
CAAGAAGCTA TACCGGATGA AGTCATCAAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CTACAAAGAA TGAATTTATT CCTACGAATT TTGAGATTTT ACCGTTAGAA 
AAAGAGGCGA CACCATACCC TGCTCTTATT AAGGATACAG CTATGAGCAA ACTTTGGTTC 
AAACAAGATG ATAAGTTTTT TTTGCCGAAG GCTTGTCTCA ACTTTGAATT TTTCAGTCGC 
TACATTTATG CTGATCCTCT CCATTGCAAC ATGACATACC TGTTTATCAG GTTATTGAAG 
GATGATTTAA AAGAGTATAC ATATGCAGCA CGCCTCTCAG GTTTGAGCTA TGGCATTGCA 
TCAGGAATGA ATGCAATACT TCTTTCAGTG AAAGGTTACA ATGACAAGCA GCCAATTTTA 
CTAAAGAAGA TTATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAAG CATATATGCG ATCTCTTAAC AATTTCCGGG CTGAACAGCC TCACCAGCAT 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAGTTAAAA 
GAAGCTCTGG ATGATGTAAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA GCTCCTGTCA 
CGGCTGCACA TTGAAGCCCT TCTCCATGGA AACATAACAA AGCAGGCTGC ATTAGGAATT 
ATGCAGATGG TTGAAGACAC CCTCATTGAA CATGCTCATA CCAAACCTCT CCTTCCAAGT 
CAGCTGGTTC GGTATAGAGA AGTTCAGCTC CCTGACAGAG GATGGTTTGT TTATCAGCAG 
AGAAATGAAG TTCACAATAA CTGTGGCATC GAGATATACT ACCAAACAGA CATGCAAAGC 
ACCTCAGAGA ATATGTTTCT GGAGCTCTTC TGTCAGATTA TCTCGGAACC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA GTTGGGCTAT ATCGTCTTCA GCGGGCCACG TCGAGCTAAT 
GGCATACAGG GCTTGAGATT CATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATTCAGGC ATTAGCAATT CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCTGAGTGTG CTAAATACTG GGGAGAAATC ATCTCCCAGC AATATAATTT TGACAGAGAT 
AACACTGAGG TTGCATATTT AAAGACACTT ACCAAGGAAG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CAGTAGATGC TCCAAGGAGA CATAAGGTAT CCGTCCATGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT TGTTGGAGAG TTCCCATGTC AAAATGACAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACCGAATT CAAGCGTGGT 
CTGCCACTGT TTCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001322793.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309722.1 Homo sapiens insulin degrading enzyme (IDE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu44273
Clone ID Related Accession (Same CDS sequence)	NM_001322793.1
Accession Version	NM_001322793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	insulin-degrading enzyme isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356128.27. On Apr 13, 2016 this sequence version replaced XM_005269766.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.43169.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309722.1
CDS	77..3136
Translation	MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001322793.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin degrading enzyme (IDE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322793.1

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTTCTGCAC CCCGCACTGC CCAGCACCTT CCGCTCAGTC 
CTCGGCGCCC GCCTGCCGCC TCCGGAGCGC CTGTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCCAT CAAGAGAATA GGAAATCACA TTACCAAGTC TCCTGAAGAC 
AAGCGAGAAT ATCGAGGGCT AGAGCTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCCACCACGG ATAAGTCATC AGCAGCACTT GATGTGCACA TAGGTTCATT GTCGGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TTTTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTTCTG TGCCCCTTGT TCGATGAAAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAAT TGGAAAAAGC TACAGGGAAT CCTAAACACC CCTTCAGTAA ATTTGGGACA 
GGTAACAAAT ATACTCTGGA GACTAGACCA AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTACTGAAAT TCCATTCTGC TTACTATTCA TCCAACTTAA TGGCTGTTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTTC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAAGATATT AGGAATCTCT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TTGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAGTCAAAGG GCTGGGTTAA TACTCTTGTT 
GGTGGGCAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACC 
GAGGAAGGAT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGACAA AGAGAGGCCA CGGGGCTATA CATCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAGGTGCTC ACAGCGGAAT ATTTACTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCCATA 
GTTTCTAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAACAAGAAG CTATACCGGA TGAAGTCATC AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCTACAAA GAATGAATTT ATTCCTACGA ATTTTGAGAT TTTACCGTTA 
GAAAAAGAGG CGACACCATA CCCTGCTCTT ATTAAGGATA CAGCTATGAG CAAACTTTGG 
TTCAAACAAG ATGATAAGTT TTTTTTGCCG AAGGCTTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTTTCA GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTACTAAAGA AGATTATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AAGCATATAT GCGATCTCTT AACAATTTCC GGGCTGAACA GCCTCACCAG 
CATGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAGTTA 
AAAGAAGCTC TGGATGATGT AACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATTGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCATTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACCAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTTTATCAG 
CAGAGAAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAAAC AGACATGCAA 
AGCACCTCAG AGAATATGTT TCTGGAGCTC TTCTGTCAGA TTATCTCGGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGTTGGGC TATATCGTCT TCAGCGGGCC ACGTCGAGCT 
AATGGCATAC AGGGCTTGAG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATTCA GGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCTGAGT GTGCTAAATA CTGGGGAGAA ATCATCTCCC AGCAATATAA TTTTGACAGA 
GATAACACTG AGGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCTCCAAGG AGACATAAGG TATCCGTCCA TGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTTGTTGGA GAGTTCCCAT GTCAAAATGA CATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACCGA ATTCAAGCGT 
GGTCTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	IDE
Entrez Gene ID	3416
Full Name	insulin degrading enzyme
Synonyms	INSULYSIN
General protein information	Preferred Names insulin degrading enzyme Names insulin-degrading enzyme Abeta-degrading protease insulin protease insulinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.33
Summary	This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009].
Disorder MIM:	146680

Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
	IDE	XP_001090249.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

IDE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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