NANOS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NANOS1
Entrez Gene ID	340719
Full Name	nanos C2HC-type zinc finger 1
Synonyms	EC_Rep1a,NOS-1,NOS1,SPGF12,ZC2HC12A
General protein information	Preferred Names nanos C2HC-type zinc finger 1 Names nanos homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.11
Summary	This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015].
Disorder MIM:	608226
Disorder Html:	Spermatogenic failure 12, 615413 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199461.4	NP_955631.1	nanos homolog 1
NM_199461.3	NP_955631.1	nanos homolog 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NANOS1	NP_955631.1
Rattus norvegicus (Norway rat)	Nanos1	XP_006231749.1
Gallus gallus (chicken)	NANOS1	XP_003641548.1
Canis lupus familiaris (dog)	NANOS1	XP_005637940.1
Bos taurus (cattle)	NANOS1	XP_005225853.1
Mus musculus (house mouse)	Nanos1	NP_848508.2

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells.
Miles WO, Korenjak M, Griffiths LM, Dyer MA, Provero P, Dyson NJ
The EMBO journal33(19)2201-15(2014 Oct)

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Bhandari D, Raisch T, Weichenrieder O, Jonas S, Izaurralde E
Genes & development28(8)888-901(2014 Apr)

Mutations of NANOS1, a human homologue of the Drosophila morphogen, are associated with a lack of germ cells in testes or severe oligo-astheno-teratozoospermia.
Kusz-Zamelczyk K, Sajek M, Spik A, Glazar R, J?drzejczak P, Latos-Biele?ska A, Kotecki M, Pawelczyk L, Jaruzelska J
Journal of medical genetics50(3)187-93(2013 Mar)

Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population.
Lu C, Xu M, Wang Y, Qin Y, Du G, Wu W, Han X, Ji C, Yang Y, Gu A, Xia Y, Song L, Wang S, Wang X
PloS one8(1)e53443(2013)

NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells.
Ginter-Matuszewska B, Kusz K, Spik A, Grzeszkowiak D, Rembiszewska A, Kupryjanczyk J, Jaruzelska J
Histochemistry and cell biology136(3)279-87(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High NANOS expression is associated with retinoblastoma.
Title: Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells.

A group of 195 patients manifesting non-obstructive azoospermia or oligozoospermia were tested for mutations of the NANOS1 gene.
Title: Mutations of NANOS1, a human homologue of the Drosophila morphogen, are associated with a lack of germ cells in testes or severe oligo-astheno-teratozoospermia.

Single nucleotide polymorphisms in CYP26B1, NANOS1 and STRA8 genes support involvement of meiotic program initiation in modifying azoospermia and oligozoospermia risk in a Han-Chinese population
Title: Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population.

NANOS1-PUMILIO2 complex, together with GEMIN3 and small noncoding RNAs, possibly regulate mRNA translation within the chromatoid body of the human germ cells.
Title: NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells.

Data demonstrated that SNAPIN interacts additionally with NANOS1 protein. This is the first report demonstrating that the N-terminal region of NANOS1 is necessary for protein binding.
Title: The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.

The following NANOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NANOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29715	NM_199461.4 Latest version!	Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$216.30 $309.00
View Old Accession Versions >>
OHu29715	NM_199461.3	Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NANOS1 ( NM_199461.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_955631.1 Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.

Price & Availability↑

CloneID	OHu29715
Clone ID Related Accession (Same CDS sequence)	NM_199461.4 , NM_199461.3
Accession Version	NM_199461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	nanos homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157788.16 and AF458985.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_199461.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AF458985.1, AF275269.2 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000425699.3/ ENSP00000393275.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCTT TCCCCTGGGC GCCCCGCTCG CCCCGCCGCG GCCGCGCCCC CCCGCCCATG 
GCGCTCGTGC CCAGCGCCCG CTACGTGAGC GCCCCGGGCC CGGCGCACCC GCAGCCCTTC 
AGCTCCTGGA ACGACTACCT GGGGCTCGCC ACGCTCATCA CCAAAGCGGT GGACGGCGAG 
CCGCGCTTCG GCTGCGCCCG CGGTGGGAAC GGCGGCGGCG GCTCCCCGCC CTCCTCCTCC 
TCGTCGTCCT GCTGCTCCCC CCACACGGGG GCCGGGCCTG GGGCGCTGGG GCCGGCGCTG 
GGGCCGCCCG ACTACGACGA GGACGACGAC GACGACAGCG ACGAGCCGGG GTCCCGGGGC 
CGCTACCTGG GGAGCGCGCT GGAATTGCGC GCGCTGGAGC TGTGCGCGGG CCCCGCCGAG 
GCCGGGCTGC TGGAGGAGCG CTTCGCCGAG CTGAGCCCGT TCGCGGGTCG TGCCGCCGCC 
GTGCTGCTGG GCTGCGCGCC CGCCGCCGCC GCCGCCGCCA CCACCACCAG CGAGGCGACG 
CCGCGCGAGG AGCGGGCCCC GGCGTGGGCG GCCGAGCCCC GGCTGCACGC GGCCTCCGGG 
GCGGCGGCCG CCCGGCTGCT GAAGCCCGAG CTGCAGGTGT GCGTGTTCTG CCGGAACAAC 
AAGGAGGCGA TGGCGCTCTA CACCACCCAT ATCCTCAAGG GCCCCGACGG GCGAGTGCTG 
TGTCCCGTGC TGCGCCGCTA CACGTGTCCC CTGTGCGGCG CCAGCGGCGA CAACGCGCAC 
ACCATCAAGT ACTGCCCGCT CTCCAAAGTG CCGCCGCCGC CCGCCCGCCC GCCGCCCCGC 
AGCGCCAGGG ACGGCCCGCC TGGCAAGAAG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955631.1
CDS	89..967
Translation	MEAFPWAPRSPRRGRAPPPMALVPSARYVSAPGPAHPQPFSSWNDYLGLATLITKAVDGEPRFGCARGGNGGGGSPPSSSSSSCCSPHTGAGPGALGPALGPPDYDEDDDDDSDEPGSRGRYLGSALELRALELCAGPAEAGLLEERFAELSPFAGRAAAVLLGCAPAAAAAATTTSEATPREERAPAWAAEPRLHAASGAAAARLLKPELQVCVFCRNNKEAMALYTTHILKGPDGRVLCPVLRRYTCPLCGASGDNAHTIKYCPLSKVPPPPARPPPRSARDGPPGKKLR

Target ORF information:

RefSeq Version	NM_199461.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199461.4

ORF Insert Sequence:

ATGGAGGCTT TCCCCTGGGC GCCCCGCTCG CCCCGCCGCG GCCGCGCCCC CCCGCCCATG 
GCGCTCGTGC CCAGCGCCCG CTACGTGAGC GCCCCGGGCC CGGCGCACCC GCAGCCCTTC 
AGCTCCTGGA ACGACTACCT GGGGCTCGCC ACGCTCATCA CCAAAGCGGT GGACGGCGAG 
CCGCGCTTCG GCTGCGCCCG CGGTGGGAAC GGCGGCGGCG GCTCCCCGCC CTCCTCCTCC 
TCGTCGTCCT GCTGCTCCCC CCACACGGGG GCCGGGCCTG GGGCGCTGGG GCCGGCGCTG 
GGGCCGCCCG ACTACGACGA GGACGACGAC GACGACAGCG ACGAGCCGGG GTCCCGGGGC 
CGCTACCTGG GGAGCGCGCT GGAATTGCGC GCGCTGGAGC TGTGCGCGGG CCCCGCCGAG 
GCCGGGCTGC TGGAGGAGCG CTTCGCCGAG CTGAGCCCGT TCGCGGGTCG TGCCGCCGCC 
GTGCTGCTGG GCTGCGCGCC CGCCGCCGCC GCCGCCGCCA CCACCACCAG CGAGGCGACG 
CCGCGCGAGG AGCGGGCCCC GGCGTGGGCG GCCGAGCCCC GGCTGCACGC GGCCTCCGGG 
GCGGCGGCCG CCCGGCTGCT GAAGCCCGAG CTGCAGGTGT GCGTGTTCTG CCGGAACAAC 
AAGGAGGCGA TGGCGCTCTA CACCACCCAT ATCCTCAAGG GCCCCGACGG GCGAGTGCTG 
TGTCCCGTGC TGCGCCGCTA CACGTGTCCC CTGTGCGGCG CCAGCGGCGA CAACGCGCAC 
ACCATCAAGT ACTGCCCGCT CTCCAAAGTG CCGCCGCCGC CCGCCCGCCC GCCGCCCCGC 
AGCGCCAGGG ACGGCCCGCC TGGCAAGAAG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NANOS1 ( NM_199461.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_955631.1 Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.

Price & Availability↑

CloneID	OHu29715
Clone ID Related Accession (Same CDS sequence)	NM_199461.4 , NM_199461.3
Accession Version	NM_199461.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	nanos homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF458985.1 and AL157788.16. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2015 this sequence version replaced NM_199461.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AF458985.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTT TCCCCTGGGC GCCCCGCTCG CCCCGCCGCG GCCGCGCCCC CCCGCCCATG 
GCGCTCGTGC CCAGCGCCCG CTACGTGAGC GCCCCGGGCC CGGCGCACCC GCAGCCCTTC 
AGCTCCTGGA ACGACTACCT GGGGCTCGCC ACGCTCATCA CCAAAGCGGT GGACGGCGAG 
CCGCGCTTCG GCTGCGCCCG CGGTGGGAAC GGCGGCGGCG GCTCCCCGCC CTCCTCCTCC 
TCGTCGTCCT GCTGCTCCCC CCACACGGGG GCCGGGCCTG GGGCGCTGGG GCCGGCGCTG 
GGGCCGCCCG ACTACGACGA GGACGACGAC GACGACAGCG ACGAGCCGGG GTCCCGGGGC 
CGCTACCTGG GGAGCGCGCT GGAATTGCGC GCGCTGGAGC TGTGCGCGGG CCCCGCCGAG 
GCCGGGCTGC TGGAGGAGCG CTTCGCCGAG CTGAGCCCGT TCGCGGGTCG TGCCGCCGCC 
GTGCTGCTGG GCTGCGCGCC CGCCGCCGCC GCCGCCGCCA CCACCACCAG CGAGGCGACG 
CCGCGCGAGG AGCGGGCCCC GGCGTGGGCG GCCGAGCCCC GGCTGCACGC GGCCTCCGGG 
GCGGCGGCCG CCCGGCTGCT GAAGCCCGAG CTGCAGGTGT GCGTGTTCTG CCGGAACAAC 
AAGGAGGCGA TGGCGCTCTA CACCACCCAT ATCCTCAAGG GCCCCGACGG GCGAGTGCTG 
TGTCCCGTGC TGCGCCGCTA CACGTGTCCC CTGTGCGGCG CCAGCGGCGA CAACGCGCAC 
ACCATCAAGT ACTGCCCGCT CTCCAAAGTG CCGCCGCCGC CCGCCCGCCC GCCGCCCCGC 
AGCGCCAGGG ACGGCCCGCC TGGCAAGAAG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955631.1
CDS	87..965
Translation	MEAFPWAPRSPRRGRAPPPMALVPSARYVSAPGPAHPQPFSSWNDYLGLATLITKAVDGEPRFGCARGGNGGGGSPPSSSSSSCCSPHTGAGPGALGPALGPPDYDEDDDDDSDEPGSRGRYLGSALELRALELCAGPAEAGLLEERFAELSPFAGRAAAVLLGCAPAAAAAATTTSEATPREERAPAWAAEPRLHAASGAAAARLLKPELQVCVFCRNNKEAMALYTTHILKGPDGRVLCPVLRRYTCPLCGASGDNAHTIKYCPLSKVPPPPARPPPRSARDGPPGKKLR

Target ORF information:

RefSeq Version	NM_199461.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nanos C2HC-type zinc finger 1 (NANOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199461.3

ORF Insert Sequence:

ATGGAGGCTT TCCCCTGGGC GCCCCGCTCG CCCCGCCGCG GCCGCGCCCC CCCGCCCATG 
GCGCTCGTGC CCAGCGCCCG CTACGTGAGC GCCCCGGGCC CGGCGCACCC GCAGCCCTTC 
AGCTCCTGGA ACGACTACCT GGGGCTCGCC ACGCTCATCA CCAAAGCGGT GGACGGCGAG 
CCGCGCTTCG GCTGCGCCCG CGGTGGGAAC GGCGGCGGCG GCTCCCCGCC CTCCTCCTCC 
TCGTCGTCCT GCTGCTCCCC CCACACGGGG GCCGGGCCTG GGGCGCTGGG GCCGGCGCTG 
GGGCCGCCCG ACTACGACGA GGACGACGAC GACGACAGCG ACGAGCCGGG GTCCCGGGGC 
CGCTACCTGG GGAGCGCGCT GGAATTGCGC GCGCTGGAGC TGTGCGCGGG CCCCGCCGAG 
GCCGGGCTGC TGGAGGAGCG CTTCGCCGAG CTGAGCCCGT TCGCGGGTCG TGCCGCCGCC 
GTGCTGCTGG GCTGCGCGCC CGCCGCCGCC GCCGCCGCCA CCACCACCAG CGAGGCGACG 
CCGCGCGAGG AGCGGGCCCC GGCGTGGGCG GCCGAGCCCC GGCTGCACGC GGCCTCCGGG 
GCGGCGGCCG CCCGGCTGCT GAAGCCCGAG CTGCAGGTGT GCGTGTTCTG CCGGAACAAC 
AAGGAGGCGA TGGCGCTCTA CACCACCCAT ATCCTCAAGG GCCCCGACGG GCGAGTGCTG 
TGTCCCGTGC TGCGCCGCTA CACGTGTCCC CTGTGCGGCG CCAGCGGCGA CAACGCGCAC 
ACCATCAAGT ACTGCCCGCT CTCCAAAGTG CCGCCGCCGC CCGCCCGCCC GCCGCCCCGC 
AGCGCCAGGG ACGGCCCGCC TGGCAAGAAG CTGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells.
The EMBO journal33(19)2201-15(2014 Oct)
Miles WO,Korenjak M,Griffiths LM,Dyer MA,Provero P,Dyson NJ

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Genes & development28(8)888-901(2014 Apr)
Bhandari D,Raisch T,Weichenrieder O,Jonas S,Izaurralde E

Mutations of NANOS1, a human homologue of the Drosophila morphogen, are associated with a lack of germ cells in testes or severe oligo-astheno-teratozoospermia.
Journal of medical genetics50(3)187-93(2013 Mar)
Kusz-Zamelczyk K,Sajek M,Spik A,Glazar R,J?drzejczak P,Latos-Biele?ska A,Kotecki M,Pawelczyk L,Jaruzelska J

Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population.
PloS one8(1)e53443(2013)
Lu C,Xu M,Wang Y,Qin Y,Du G,Wu W,Han X,Ji C,Yang Y,Gu A,Xia Y,Song L,Wang S,Wang X

NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells.
Histochemistry and cell biology136(3)279-87(2011 Sep)
Ginter-Matuszewska B,Kusz K,Spik A,Grzeszkowiak D,Rembiszewska A,Kupryjanczyk J,Jaruzelska J

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NANOS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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