IARS1 cDNA ORF clone, Homo sapiens(human)

The following IARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu108895 NM_001374299.1
Latest version!
Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu108895 NM_001374300.1
Latest version!
Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu108896 NM_001374301.1
Latest version!
Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu24821 NM_013417.4
Latest version!
Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu24821 NM_002161.6
Latest version!
Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OHu24821 NM_013417.3 Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu24821 NM_002161.5 Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu108895
    Clone ID Related Accession (Same CDS sequence) NM_001374300.1 , NM_001374299.1
    Accession Version NM_001374300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.146576.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG
    CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG
    CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT
    CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT
    TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA
    AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG
    AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG
    CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC
    CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA
    GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA
    ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA
    ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT
    ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA
    GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG
    GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT
    AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT
    GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA
    AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG
    AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT
    TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG
    TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC
    ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA
    AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT
    TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361229.1
    CDS265..3978
    Translation

    Target ORF information:

    RefSeq Version NM_001374300.1
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG
    CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG
    CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT
    CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT
    TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA
    AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG
    AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG
    CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC
    CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA
    GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA
    ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA
    ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT
    ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA
    GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG
    GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT
    AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT
    GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA
    AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG
    AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT
    TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG
    TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC
    ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA
    AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT
    TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108895
    Clone ID Related Accession (Same CDS sequence) NM_001374300.1 , NM_001374299.1
    Accession Version NM_001374299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.153835.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG
    CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG
    CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT
    CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT
    TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA
    AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG
    AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG
    CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC
    CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA
    GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA
    ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA
    ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT
    ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA
    GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG
    GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT
    AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT
    GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA
    AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG
    AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT
    TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG
    TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC
    ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA
    AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT
    TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361228.1
    CDS95..3808
    Translation

    Target ORF information:

    RefSeq Version NM_001374299.1
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG
    CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG
    CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT
    CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT
    TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA
    AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG
    AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG
    CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC
    CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA
    GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA
    ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA
    ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT
    ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA
    GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG
    GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT
    AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT
    GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA
    AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG
    AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT
    TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG
    TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC
    ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA
    AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT
    TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108896
    Clone ID Related Accession (Same CDS sequence) NM_001374301.1
    Accession Version NM_001374301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.129230.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGATT ATATCTGATT
    AACTCCCCTG TGGTGAGAGC AGAAAACCTC CGCTTTAAAG AAGAGGGTGT GCGGGACGTC
    CTTAAGGATG TACTGCTCCC ATGGTACAAT GCCTATCGCT TCTTAATCCA GAACGTTCTG
    AGGCTCCAGA AGGAGGAAGA AATAGAATTT CTCTACAATG AGAACACGGT TAGAGAAAGC
    CCCAACATTA CAGACCGGTG GATCCTGTCC TTCATGCAGT CTCTCATTGG CTTCTTTGAG
    ACTGAAATGG CAGCTTATAG GCTTTATACT GTGGTGCCTC GCCTGGTCAA GTTTGTAGAT
    ATTCTGACCA ATTGGTATGT TAGAATGAAC CGCAGAAGAT TAAAGGGTGA AAATGGGATG
    GAGGATTGTG TCATGGCCCT AGAAACCTTG TTTAGTGTTC TGCTTTCTCT TTGCAGACTT
    ATGGCTCCCT ACACACCTTT TCTCACTGAA TTGATGTACC AGAATCTAAA GGTGCTGATT
    GACCCTGTTT CTGTTCAGGA CAAGGACACA CTCAGCATTC ACTACCTCAT GCTGCCCCGT
    GTTCGAGAAG AATTGATTGA CAAGAAAACA GAGAGTGCAG TATCTCAGAT GCAGTCTGTG
    ATTGAACTTG GAAGAGTGAT CAGAGACCGA AAAACTATTC CCATAAAGTA TCCTTTGAAA
    GAAATTGTGG TTATCCATCA AGATCCAGAA GCTCTTAAAG ATATCAAGTC TTTGGAGAAG
    TATATCATTG AGGAACTCAA TGTTCGAAAA GTTACACTGT CTACAGATAA AAACAAGTAT
    GGCATTCGGC TAAGGGCAGA ACCAGATCAC ATGGTCCTGG GGAAGCGTCT GAAGGGAGCC
    TTTAAGGCAG TGATGACGTC CATCAAGCAG TTGAGCAGTG AGGAGCTGGA GCAGTTCCAG
    AAGACTGGGA CCATTGTTGT GGAAGGCCAT GAATTGCACG ATGAAGACAT CCGCCTCATG
    TACACCTTTG ATCAGGCCAC AGGTGGGACT GCGCAATTTG AAGCACACTC AGATGCTCAG
    GCTTTGGTCC TCTTAGATGT CACTCCTGAC CAGTCAATGG TAGATGAAGG AATGGCTCGG
    GAAGTCATCA ATCGCATACA GAAACTTCGC AAAAAGTGCA ATCTGGTTCC AACTGATGAA
    ATCACAGTGT ACTATAAAGC AAAGTCTGAA GGAACATATC TGAATAGTGT TATTGAAAGC
    CACACAGAGT TCATATTTAC CACCATAAAG GCTCCCTTGA AACCATATCC AGTTTCTCCA
    TCGGATAAAG TCCTTATTCA AGAAAAAACA CAGTTGAAGG GATCTGAACT GGAAATTACA
    CTCACCAGAG GATCTTCCCT TCCTGGTCCT GCTTGTGCAT ATGTCAATCT TAACATTTGT
    GCAAATGGCA GTGAACAAGG TGGAGTATTG CTCCTGGAAA ATCCAAAAGG TGACAATAGG
    TTGGACCTTT TAAAGCTGAA GAGTGTTGTC ACTAGCATTT TTGGTGTGAA AAATACAGAG
    CTGGCTGTCT TCCATGATGA AACAGAAATA CAAAACCAAA CTGACTTACT GAGTCTTAGT
    GGAAAAACAC TTTGTGTGAC TGCAGGATCG GCTCCCTCTC TGATCAACAG TTCTAGTACT
    CTTCTTTGTC AGTATATCAA CCTACAGCTC CTGAATGCAA AGCCACAAGA GTGTTTAATG
    GGGACAGTGG GCACTCTCCT GCTTGAAAAC CCACTTGGGC AGAATGGACT CACCCACCAA
    GGTCTTCTGT ATGAAGCAGC CAAGGTGTTT GGCCTTCGGA GCAGGAAGCT AAAGCTGTTT
    CTGAATGAGA CCCAAACGCA GGAAATTACA GAAGACATCC CCGTGAAGAC TTTGAATATG
    AAGACTGTGT ATGTTTCTGT GTTACCAACA ACAGCAGACT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361230.1
    CDS95..3799
    Translation

    Target ORF information:

    RefSeq Version NM_001374301.1
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGATT ATATCTGATT
    AACTCCCCTG TGGTGAGAGC AGAAAACCTC CGCTTTAAAG AAGAGGGTGT GCGGGACGTC
    CTTAAGGATG TACTGCTCCC ATGGTACAAT GCCTATCGCT TCTTAATCCA GAACGTTCTG
    AGGCTCCAGA AGGAGGAAGA AATAGAATTT CTCTACAATG AGAACACGGT TAGAGAAAGC
    CCCAACATTA CAGACCGGTG GATCCTGTCC TTCATGCAGT CTCTCATTGG CTTCTTTGAG
    ACTGAAATGG CAGCTTATAG GCTTTATACT GTGGTGCCTC GCCTGGTCAA GTTTGTAGAT
    ATTCTGACCA ATTGGTATGT TAGAATGAAC CGCAGAAGAT TAAAGGGTGA AAATGGGATG
    GAGGATTGTG TCATGGCCCT AGAAACCTTG TTTAGTGTTC TGCTTTCTCT TTGCAGACTT
    ATGGCTCCCT ACACACCTTT TCTCACTGAA TTGATGTACC AGAATCTAAA GGTGCTGATT
    GACCCTGTTT CTGTTCAGGA CAAGGACACA CTCAGCATTC ACTACCTCAT GCTGCCCCGT
    GTTCGAGAAG AATTGATTGA CAAGAAAACA GAGAGTGCAG TATCTCAGAT GCAGTCTGTG
    ATTGAACTTG GAAGAGTGAT CAGAGACCGA AAAACTATTC CCATAAAGTA TCCTTTGAAA
    GAAATTGTGG TTATCCATCA AGATCCAGAA GCTCTTAAAG ATATCAAGTC TTTGGAGAAG
    TATATCATTG AGGAACTCAA TGTTCGAAAA GTTACACTGT CTACAGATAA AAACAAGTAT
    GGCATTCGGC TAAGGGCAGA ACCAGATCAC ATGGTCCTGG GGAAGCGTCT GAAGGGAGCC
    TTTAAGGCAG TGATGACGTC CATCAAGCAG TTGAGCAGTG AGGAGCTGGA GCAGTTCCAG
    AAGACTGGGA CCATTGTTGT GGAAGGCCAT GAATTGCACG ATGAAGACAT CCGCCTCATG
    TACACCTTTG ATCAGGCCAC AGGTGGGACT GCGCAATTTG AAGCACACTC AGATGCTCAG
    GCTTTGGTCC TCTTAGATGT CACTCCTGAC CAGTCAATGG TAGATGAAGG AATGGCTCGG
    GAAGTCATCA ATCGCATACA GAAACTTCGC AAAAAGTGCA ATCTGGTTCC AACTGATGAA
    ATCACAGTGT ACTATAAAGC AAAGTCTGAA GGAACATATC TGAATAGTGT TATTGAAAGC
    CACACAGAGT TCATATTTAC CACCATAAAG GCTCCCTTGA AACCATATCC AGTTTCTCCA
    TCGGATAAAG TCCTTATTCA AGAAAAAACA CAGTTGAAGG GATCTGAACT GGAAATTACA
    CTCACCAGAG GATCTTCCCT TCCTGGTCCT GCTTGTGCAT ATGTCAATCT TAACATTTGT
    GCAAATGGCA GTGAACAAGG TGGAGTATTG CTCCTGGAAA ATCCAAAAGG TGACAATAGG
    TTGGACCTTT TAAAGCTGAA GAGTGTTGTC ACTAGCATTT TTGGTGTGAA AAATACAGAG
    CTGGCTGTCT TCCATGATGA AACAGAAATA CAAAACCAAA CTGACTTACT GAGTCTTAGT
    GGAAAAACAC TTTGTGTGAC TGCAGGATCG GCTCCCTCTC TGATCAACAG TTCTAGTACT
    CTTCTTTGTC AGTATATCAA CCTACAGCTC CTGAATGCAA AGCCACAAGA GTGTTTAATG
    GGGACAGTGG GCACTCTCCT GCTTGAAAAC CCACTTGGGC AGAATGGACT CACCCACCAA
    GGTCTTCTGT ATGAAGCAGC CAAGGTGTTT GGCCTTCGGA GCAGGAAGCT AAAGCTGTTT
    CTGAATGAGA CCCAAACGCA GGAAATTACA GAAGACATCC CCGTGAAGAC TTTGAATATG
    AAGACTGTGT ATGTTTCTGT GTTACCAACA ACAGCAGACT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24821
    Clone ID Related Accession (Same CDS sequence) NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
    Accession Version NM_013417.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. On Sep 19, 2019 this sequence version replaced NM_013417.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D28473.1, U04953.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_038203.2
    CDS265..4053
    Translation

    Target ORF information:

    RefSeq Version NM_013417.4
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013417.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24821
    Clone ID Related Accession (Same CDS sequence) NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
    Accession Version NM_002161.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. On Oct 11, 2019 this sequence version replaced NM_002161.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293014.1, BX647236.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002152.2
    CDS95..3883
    Translation

    Target ORF information:

    RefSeq Version NM_002161.6
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002161.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24821
    Clone ID Related Accession (Same CDS sequence) NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
    Accession Version NM_013417.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA335066.1, D28473.1 and AA731323.1. This sequence is a reference standard in the RefSeqGene project. On Nov 21, 2012 this sequence version replaced NM_013417.2. Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.169974.1, SRR1803616.208815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_038203.2
    CDS324..4112
    Translation

    Target ORF information:

    RefSeq Version NM_013417.3
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013417.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24821
    Clone ID Related Accession (Same CDS sequence) NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
    Accession Version NM_002161.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA335066.1, BC065552.1, BC008318.1 and AA731323.1. On Nov 21, 2012 this sequence version replaced NM_002161.4. Transcript Variant: This variant (1) and variant 2 encode the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.29740.1, SRR1803616.98679.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002152.2
    CDS154..3942
    Translation

    Target ORF information:

    RefSeq Version NM_002161.5
    Organism Homo sapiens(human)
    Definition Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002161.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
    TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA
    TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT
    GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA
    GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA
    GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA
    GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA
    GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG
    GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC
    ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG
    GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT
    TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC
    TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG
    AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA
    CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC
    ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA
    GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC
    TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC
    CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC
    ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA
    CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT
    AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT
    CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC
    AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG
    CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG
    CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA
    AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT
    GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG
    TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC
    ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT
    GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT
    ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT
    AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA
    CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC
    AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA
    TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA
    ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA
    TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC
    TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG
    AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG
    GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC
    ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC
    CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC
    TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT
    GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT
    GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC
    ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA
    GACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.