HSPA9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA9
Entrez Gene ID	3313
Full Name	heat shock protein family A (Hsp70) member 9
Synonyms	CRP40,CSA,EVPLS,GRP-75,GRP75,HEL-S-124m,HSPA9B,MOT,MOT2,MTHSP75,PBP74,SAAN,SIDBA4
General protein information	Preferred Names heat shock protein family A (Hsp70) member 9 Names stress-70 protein, mitochondrial 75 kDa glucose-regulated protein catecholamine-regulated protein 40 epididymis secretory sperm binding protein Li 124m heat shock 70kD protein 9B heat shock 70kDa protein 9 (mortalin) mortalin, perinuclear mortalin-2 mortalin2 p66-mortalin peptide-binding protein 74
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.2
Summary	This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010].
Disorder MIM:	600548
Disorder Html:	Anemia, sideroblastic, 4, 182170 (3); Even-plus syndrome, 616854 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004134.7	NP_004125.3	stress-70 protein, mitochondrial precursor
NM_004134.6	NP_004125.3	stress-70 protein, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HSPA9	XP_001171426.2
Bos taurus (cattle)	HSPA9	NP_001029696.1
Gallus gallus (chicken)	HSPA9	NP_001006147.1
Schizosaccharomyces pombe (fission yeast)	ssc1	NP_593459.1
Arabidopsis thaliana (thale cress)	mtHsc70-1	NP_195504.2
Homo sapiens (human)	HSPA9	NP_004125.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010876	XP_309825.4
Kluyveromyces lactis	KLLA0E22309g	XP_454960.1
	HSPA9	XP_001113234.1
Canis lupus familiaris (dog)	HSPA9	XP_531923.2
Mus musculus (house mouse)	Hspa9	NP_034611.2
Danio rerio (zebrafish)	hspa9	NP_958483.2
Saccharomyces cerevisiae (baker's yeast)	SSC1	NP_012579.1
Neurospora crassa	NCU08693	XP_961753.2
Oryza sativa (rice)	Os09g0491772	NP_001175918.1
Xenopus tropicalis (tropical clawed frog)	hspa9	NP_001001229.1
Rattus norvegicus (Norway rat)	Hspa9	NP_001094128.2
Drosophila melanogaster (fruit fly)	Hsc70-5	NP_523741.2
Caenorhabditis elegans (roundworm)	hsp-6	NP_504291.1
Eremothecium gossypii	AGOS_AFR352C	NP_985899.2
Magnaporthe oryzae (rice blast fungus)	MGG_04191	XP_003719632.1

Gene Ontology
Bibliography

Related articles in PubMed

Steady-State Levels of Phosphorylated Mitogen-Activated Protein Kinase Kinase 1/2 Determined by Mortalin/HSPA9 and Protein Phosphatase 1 Alpha in
Wu PK, Hong SK, Park JI
Molecular and cellular biology37(18)(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mortalin (HSPA9) directly interacts with PP1alpha and MEK1/2 and facilitates PP1alpha-mediated dephosphorylation of MEK1/2 by promoting their physical interaction, which is important in determining cellular steady-state levels of phosphorylated MEK1/2 and, subsequently, MEK/ERK activity.
Title: Steady-State Levels of Phosphorylated Mitogen-Activated Protein Kinase Kinase 1/2 Determined by Mortalin/HSPA9 and Protein Phosphatase 1 Alpha in

Identify Mortalin as a significant independent prognostic factor, in addition to location, clinical stage, and perineural invasion, for survival of pancreatic ductal adenocarcinoma patients.
Title: Mortalin expression in pancreatic cancer and its clinical and prognostic significance.

Mortalin is up-regulated in non-small cell lung cancer, and it may be a potential biomarker of prognostic evaluation and a molecular therapeutic target for patients with early stage of non-small cell lung cancer
Title: Mortalin overexpression predicts poor prognosis in early stage of non-small cell lung cancer.

these findings indicate that reduced levels of HSPA9 may contribute to TP53 activation and increased apoptosis observed in del(5q)-associated MDS.
Title: Knockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells.

role of HAPA9 and its interaction with other mitochondria-related genes are not genetic risk factors for PD or AD.
Title: Lack of association of mortalin (HSPA9) and other mitochondria-related genes with risk of Parkinson's and Alzheimer's diseases.

The following HSPA9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21795	NM_004134.7 Latest version!	Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu21795	NM_004134.6	Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA9 ( NM_004134.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_004125.3 Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.

Price & Availability↑

CloneID	OHu21795
Clone ID Related Accession (Same CDS sequence)	NM_004134.7 , NM_004134.6
Accession Version	NM_004134.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	stress-70 protein, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315177.1, AC011385.6 and AK225488.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004134.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.77555.1, SRR1803611.243767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000297185.9/ ENSP00000297185.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATAAGTG CCAGCCGAGC TGCAGCAGCC CGTCTCGTGG GCGCCGCAGC CTCCCGGGGC 
CCTACGGCCG CCCGCCACCA GGATAGCTGG AATGGCCTTA GTCATGAGGC TTTTAGACTT 
GTTTCAAGGC GGGATTATGC ATCAGAAGCA ATCAAGGGAG CAGTTGTTGG TATTGATTTG 
GGTACTACCA ACTCCTGCGT GGCAGTTATG GAAGGTAAAC AAGCAAAGGT GCTGGAGAAT 
GCCGAAGGTG CCAGAACCAC CCCTTCAGTT GTGGCCTTTA CAGCAGATGG TGAGCGACTT 
GTTGGAATGC CGGCCAAGCG ACAGGCTGTC ACCAACCCAA ACAATACATT TTATGCTACC 
AAGCGTCTCA TTGGCCGGCG ATATGATGAT CCTGAAGTAC AGAAAGACAT TAAAAATGTT 
CCCTTTAAAA TTGTCCGTGC CTCCAATGGT GATGCCTGGG TTGAGGCTCA TGGGAAATTG 
TATTCTCCGA GTCAGATTGG AGCATTTGTG TTGATGAAGA TGAAAGAGAC TGCAGAAAAT 
TACTTGGGGC ACACAGCAAA AAATGCTGTG ATCACAGTCC CAGCTTATTT CAATGACTCG 
CAGAGACAGG CCACTAAAGA TGCTGGCCAG ATATCTGGAC TGAATGTGCT TCGGGTGATT 
AATGAGCCCA CAGCTGCTGC TCTTGCCTAT GGTCTAGACA AATCAGAAGA CAAAGTCATT 
GCTGTATATG ATTTAGGTGG TGGAACTTTT GATATTTCTA TCCTGGAAAT TCAGAAAGGA 
GTATTTGAGG TGAAATCCAC AAATGGGGAT ACCTTCTTAG GTGGGGAAGA CTTTGACCAG 
GCCTTGCTAC GGCACATTGT GAAGGAGTTC AAGAGAGAGA CAGGGGTTGA TTTGACTAAA 
GACAACATGG CACTTCAGAG GGTACGGGAA GCTGCTGAAA AGGCTAAATG TGAACTCTCC 
TCATCTGTGC AGACTGACAT CAATTTGCCC TATCTTACAA TGGATTCTTC TGGACCCAAG 
CATTTGAATA TGAAGTTGAC CCGTGCTCAA TTTGAAGGGA TTGTCACTGA TCTAATCAGA 
AGGACTATCG CTCCATGCCA AAAAGCTATG CAAGATGCAG AAGTCAGCAA GAGTGACATA 
GGAGAAGTGA TTCTTGTGGG TGGCATGACT AGGATGCCCA AGGTTCAGCA GACTGTACAG 
GATCTTTTTG GCAGAGCCCC AAGTAAAGCT GTCAATCCTG ATGAGGCTGT GGCCATTGGA 
GCTGCCATTC AGGGAGGTGT GTTGGCCGGC GATGTCACGG ATGTGCTGCT CCTTGATGTC 
ACTCCCCTGT CTCTGGGTAT TGAAACTCTA GGAGGTGTCT TTACCAAACT TATTAATAGG 
AATACCACTA TTCCAACCAA GAAGAGCCAG GTATTCTCTA CTGCCGCTGA TGGTCAAACG 
CAAGTGGAAA TTAAAGTGTG TCAGGGTGAA AGAGAGATGG CTGGAGACAA CAAACTCCTT 
GGACAGTTTA CTTTGATTGG AATTCCACCA GCCCCTCGTG GAGTTCCTCA GATTGAAGTT 
ACATTTGACA TTGATGCCAA TGGGATAGTA CATGTTTCTG CTAAAGATAA AGGCACAGGA 
CGTGAGCAGC AGATTGTAAT CCAGTCTTCT GGTGGATTAA GCAAAGATGA TATTGAAAAT 
ATGGTTAAAA ATGCAGAGAA ATATGCTGAA GAAGACCGGC GAAAGAAGGA ACGAGTTGAA 
GCAGTTAATA TGGCTGAAGG AATCATTCAC GACACAGAAA CCAAGATGGA AGAATTCAAG 
GACCAATTAC CTGCTGATGA GTGCAACAAG CTGAAAGAAG AGATTTCCAA AATGAGGGAG 
CTCCTGGCTA GAAAAGACAG CGAAACAGGA GAAAATATTA GACAGGCAGC ATCCTCTCTT 
CAGCAGGCAT CACTGAAGCT GTTCGAAATG GCATACAAAA AGATGGCATC TGAGCGAGAA 
GGCTCTGGAA GTTCTGGCAC TGGGGAACAA AAGGAAGATC AAAAGGAGGA AAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004125.3
CDS	84..2123
Translation	MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

Target ORF information:

RefSeq Version	NM_004134.7
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004134.7

ORF Insert Sequence:

ATGATAAGTG CCAGCCGAGC TGCAGCAGCC CGTCTCGTGG GCGCCGCAGC CTCCCGGGGC 
CCTACGGCCG CCCGCCACCA GGATAGCTGG AATGGCCTTA GTCATGAGGC TTTTAGACTT 
GTTTCAAGGC GGGATTATGC ATCAGAAGCA ATCAAGGGAG CAGTTGTTGG TATTGATTTG 
GGTACTACCA ACTCCTGCGT GGCAGTTATG GAAGGTAAAC AAGCAAAGGT GCTGGAGAAT 
GCCGAAGGTG CCAGAACCAC CCCTTCAGTT GTGGCCTTTA CAGCAGATGG TGAGCGACTT 
GTTGGAATGC CGGCCAAGCG ACAGGCTGTC ACCAACCCAA ACAATACATT TTATGCTACC 
AAGCGTCTCA TTGGCCGGCG ATATGATGAT CCTGAAGTAC AGAAAGACAT TAAAAATGTT 
CCCTTTAAAA TTGTCCGTGC CTCCAATGGT GATGCCTGGG TTGAGGCTCA TGGGAAATTG 
TATTCTCCGA GTCAGATTGG AGCATTTGTG TTGATGAAGA TGAAAGAGAC TGCAGAAAAT 
TACTTGGGGC ACACAGCAAA AAATGCTGTG ATCACAGTCC CAGCTTATTT CAATGACTCG 
CAGAGACAGG CCACTAAAGA TGCTGGCCAG ATATCTGGAC TGAATGTGCT TCGGGTGATT 
AATGAGCCCA CAGCTGCTGC TCTTGCCTAT GGTCTAGACA AATCAGAAGA CAAAGTCATT 
GCTGTATATG ATTTAGGTGG TGGAACTTTT GATATTTCTA TCCTGGAAAT TCAGAAAGGA 
GTATTTGAGG TGAAATCCAC AAATGGGGAT ACCTTCTTAG GTGGGGAAGA CTTTGACCAG 
GCCTTGCTAC GGCACATTGT GAAGGAGTTC AAGAGAGAGA CAGGGGTTGA TTTGACTAAA 
GACAACATGG CACTTCAGAG GGTACGGGAA GCTGCTGAAA AGGCTAAATG TGAACTCTCC 
TCATCTGTGC AGACTGACAT CAATTTGCCC TATCTTACAA TGGATTCTTC TGGACCCAAG 
CATTTGAATA TGAAGTTGAC CCGTGCTCAA TTTGAAGGGA TTGTCACTGA TCTAATCAGA 
AGGACTATCG CTCCATGCCA AAAAGCTATG CAAGATGCAG AAGTCAGCAA GAGTGACATA 
GGAGAAGTGA TTCTTGTGGG TGGCATGACT AGGATGCCCA AGGTTCAGCA GACTGTACAG 
GATCTTTTTG GCAGAGCCCC AAGTAAAGCT GTCAATCCTG ATGAGGCTGT GGCCATTGGA 
GCTGCCATTC AGGGAGGTGT GTTGGCCGGC GATGTCACGG ATGTGCTGCT CCTTGATGTC 
ACTCCCCTGT CTCTGGGTAT TGAAACTCTA GGAGGTGTCT TTACCAAACT TATTAATAGG 
AATACCACTA TTCCAACCAA GAAGAGCCAG GTATTCTCTA CTGCCGCTGA TGGTCAAACG 
CAAGTGGAAA TTAAAGTGTG TCAGGGTGAA AGAGAGATGG CTGGAGACAA CAAACTCCTT 
GGACAGTTTA CTTTGATTGG AATTCCACCA GCCCCTCGTG GAGTTCCTCA GATTGAAGTT 
ACATTTGACA TTGATGCCAA TGGGATAGTA CATGTTTCTG CTAAAGATAA AGGCACAGGA 
CGTGAGCAGC AGATTGTAAT CCAGTCTTCT GGTGGATTAA GCAAAGATGA TATTGAAAAT 
ATGGTTAAAA ATGCAGAGAA ATATGCTGAA GAAGACCGGC GAAAGAAGGA ACGAGTTGAA 
GCAGTTAATA TGGCTGAAGG AATCATTCAC GACACAGAAA CCAAGATGGA AGAATTCAAG 
GACCAATTAC CTGCTGATGA GTGCAACAAG CTGAAAGAAG AGATTTCCAA AATGAGGGAG 
CTCCTGGCTA GAAAAGACAG CGAAACAGGA GAAAATATTA GACAGGCAGC ATCCTCTCTT 
CAGCAGGCAT CACTGAAGCT GTTCGAAATG GCATACAAAA AGATGGCATC TGAGCGAGAA 
GGCTCTGGAA GTTCTGGCAC TGGGGAACAA AAGGAAGATC AAAAGGAGGA AAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA9 ( NM_004134.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004125.3 Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.

Price & Availability↑

CloneID	OHu21795
Clone ID Related Accession (Same CDS sequence)	NM_004134.7 , NM_004134.6
Accession Version	NM_004134.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	stress-70 protein, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC356852.1, AC113340.2, AK315177.1, AC011385.6 and AK225488.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2010 this sequence version replaced NM_004134.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.60910.1, SRR1803615.118059.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATAAGTG CCAGCCGAGC TGCAGCAGCC CGTCTCGTGG GCGCCGCAGC CTCCCGGGGC 
CCTACGGCCG CCCGCCACCA GGATAGCTGG AATGGCCTTA GTCATGAGGC TTTTAGACTT 
GTTTCAAGGC GGGATTATGC ATCAGAAGCA ATCAAGGGAG CAGTTGTTGG TATTGATTTG 
GGTACTACCA ACTCCTGCGT GGCAGTTATG GAAGGTAAAC AAGCAAAGGT GCTGGAGAAT 
GCCGAAGGTG CCAGAACCAC CCCTTCAGTT GTGGCCTTTA CAGCAGATGG TGAGCGACTT 
GTTGGAATGC CGGCCAAGCG ACAGGCTGTC ACCAACCCAA ACAATACATT TTATGCTACC 
AAGCGTCTCA TTGGCCGGCG ATATGATGAT CCTGAAGTAC AGAAAGACAT TAAAAATGTT 
CCCTTTAAAA TTGTCCGTGC CTCCAATGGT GATGCCTGGG TTGAGGCTCA TGGGAAATTG 
TATTCTCCGA GTCAGATTGG AGCATTTGTG TTGATGAAGA TGAAAGAGAC TGCAGAAAAT 
TACTTGGGGC ACACAGCAAA AAATGCTGTG ATCACAGTCC CAGCTTATTT CAATGACTCG 
CAGAGACAGG CCACTAAAGA TGCTGGCCAG ATATCTGGAC TGAATGTGCT TCGGGTGATT 
AATGAGCCCA CAGCTGCTGC TCTTGCCTAT GGTCTAGACA AATCAGAAGA CAAAGTCATT 
GCTGTATATG ATTTAGGTGG TGGAACTTTT GATATTTCTA TCCTGGAAAT TCAGAAAGGA 
GTATTTGAGG TGAAATCCAC AAATGGGGAT ACCTTCTTAG GTGGGGAAGA CTTTGACCAG 
GCCTTGCTAC GGCACATTGT GAAGGAGTTC AAGAGAGAGA CAGGGGTTGA TTTGACTAAA 
GACAACATGG CACTTCAGAG GGTACGGGAA GCTGCTGAAA AGGCTAAATG TGAACTCTCC 
TCATCTGTGC AGACTGACAT CAATTTGCCC TATCTTACAA TGGATTCTTC TGGACCCAAG 
CATTTGAATA TGAAGTTGAC CCGTGCTCAA TTTGAAGGGA TTGTCACTGA TCTAATCAGA 
AGGACTATCG CTCCATGCCA AAAAGCTATG CAAGATGCAG AAGTCAGCAA GAGTGACATA 
GGAGAAGTGA TTCTTGTGGG TGGCATGACT AGGATGCCCA AGGTTCAGCA GACTGTACAG 
GATCTTTTTG GCAGAGCCCC AAGTAAAGCT GTCAATCCTG ATGAGGCTGT GGCCATTGGA 
GCTGCCATTC AGGGAGGTGT GTTGGCCGGC GATGTCACGG ATGTGCTGCT CCTTGATGTC 
ACTCCCCTGT CTCTGGGTAT TGAAACTCTA GGAGGTGTCT TTACCAAACT TATTAATAGG 
AATACCACTA TTCCAACCAA GAAGAGCCAG GTATTCTCTA CTGCCGCTGA TGGTCAAACG 
CAAGTGGAAA TTAAAGTGTG TCAGGGTGAA AGAGAGATGG CTGGAGACAA CAAACTCCTT 
GGACAGTTTA CTTTGATTGG AATTCCACCA GCCCCTCGTG GAGTTCCTCA GATTGAAGTT 
ACATTTGACA TTGATGCCAA TGGGATAGTA CATGTTTCTG CTAAAGATAA AGGCACAGGA 
CGTGAGCAGC AGATTGTAAT CCAGTCTTCT GGTGGATTAA GCAAAGATGA TATTGAAAAT 
ATGGTTAAAA ATGCAGAGAA ATATGCTGAA GAAGACCGGC GAAAGAAGGA ACGAGTTGAA 
GCAGTTAATA TGGCTGAAGG AATCATTCAC GACACAGAAA CCAAGATGGA AGAATTCAAG 
GACCAATTAC CTGCTGATGA GTGCAACAAG CTGAAAGAAG AGATTTCCAA AATGAGGGAG 
CTCCTGGCTA GAAAAGACAG CGAAACAGGA GAAAATATTA GACAGGCAGC ATCCTCTCTT 
CAGCAGGCAT CACTGAAGCT GTTCGAAATG GCATACAAAA AGATGGCATC TGAGCGAGAA 
GGCTCTGGAA GTTCTGGCAC TGGGGAACAA AAGGAAGATC AAAAGGAGGA AAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004125.3
CDS	312..2351
Translation	MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

Target ORF information:

RefSeq Version	NM_004134.6
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004134.6

ORF Insert Sequence:

ATGATAAGTG CCAGCCGAGC TGCAGCAGCC CGTCTCGTGG GCGCCGCAGC CTCCCGGGGC 
CCTACGGCCG CCCGCCACCA GGATAGCTGG AATGGCCTTA GTCATGAGGC TTTTAGACTT 
GTTTCAAGGC GGGATTATGC ATCAGAAGCA ATCAAGGGAG CAGTTGTTGG TATTGATTTG 
GGTACTACCA ACTCCTGCGT GGCAGTTATG GAAGGTAAAC AAGCAAAGGT GCTGGAGAAT 
GCCGAAGGTG CCAGAACCAC CCCTTCAGTT GTGGCCTTTA CAGCAGATGG TGAGCGACTT 
GTTGGAATGC CGGCCAAGCG ACAGGCTGTC ACCAACCCAA ACAATACATT TTATGCTACC 
AAGCGTCTCA TTGGCCGGCG ATATGATGAT CCTGAAGTAC AGAAAGACAT TAAAAATGTT 
CCCTTTAAAA TTGTCCGTGC CTCCAATGGT GATGCCTGGG TTGAGGCTCA TGGGAAATTG 
TATTCTCCGA GTCAGATTGG AGCATTTGTG TTGATGAAGA TGAAAGAGAC TGCAGAAAAT 
TACTTGGGGC ACACAGCAAA AAATGCTGTG ATCACAGTCC CAGCTTATTT CAATGACTCG 
CAGAGACAGG CCACTAAAGA TGCTGGCCAG ATATCTGGAC TGAATGTGCT TCGGGTGATT 
AATGAGCCCA CAGCTGCTGC TCTTGCCTAT GGTCTAGACA AATCAGAAGA CAAAGTCATT 
GCTGTATATG ATTTAGGTGG TGGAACTTTT GATATTTCTA TCCTGGAAAT TCAGAAAGGA 
GTATTTGAGG TGAAATCCAC AAATGGGGAT ACCTTCTTAG GTGGGGAAGA CTTTGACCAG 
GCCTTGCTAC GGCACATTGT GAAGGAGTTC AAGAGAGAGA CAGGGGTTGA TTTGACTAAA 
GACAACATGG CACTTCAGAG GGTACGGGAA GCTGCTGAAA AGGCTAAATG TGAACTCTCC 
TCATCTGTGC AGACTGACAT CAATTTGCCC TATCTTACAA TGGATTCTTC TGGACCCAAG 
CATTTGAATA TGAAGTTGAC CCGTGCTCAA TTTGAAGGGA TTGTCACTGA TCTAATCAGA 
AGGACTATCG CTCCATGCCA AAAAGCTATG CAAGATGCAG AAGTCAGCAA GAGTGACATA 
GGAGAAGTGA TTCTTGTGGG TGGCATGACT AGGATGCCCA AGGTTCAGCA GACTGTACAG 
GATCTTTTTG GCAGAGCCCC AAGTAAAGCT GTCAATCCTG ATGAGGCTGT GGCCATTGGA 
GCTGCCATTC AGGGAGGTGT GTTGGCCGGC GATGTCACGG ATGTGCTGCT CCTTGATGTC 
ACTCCCCTGT CTCTGGGTAT TGAAACTCTA GGAGGTGTCT TTACCAAACT TATTAATAGG 
AATACCACTA TTCCAACCAA GAAGAGCCAG GTATTCTCTA CTGCCGCTGA TGGTCAAACG 
CAAGTGGAAA TTAAAGTGTG TCAGGGTGAA AGAGAGATGG CTGGAGACAA CAAACTCCTT 
GGACAGTTTA CTTTGATTGG AATTCCACCA GCCCCTCGTG GAGTTCCTCA GATTGAAGTT 
ACATTTGACA TTGATGCCAA TGGGATAGTA CATGTTTCTG CTAAAGATAA AGGCACAGGA 
CGTGAGCAGC AGATTGTAAT CCAGTCTTCT GGTGGATTAA GCAAAGATGA TATTGAAAAT 
ATGGTTAAAA ATGCAGAGAA ATATGCTGAA GAAGACCGGC GAAAGAAGGA ACGAGTTGAA 
GCAGTTAATA TGGCTGAAGG AATCATTCAC GACACAGAAA CCAAGATGGA AGAATTCAAG 
GACCAATTAC CTGCTGATGA GTGCAACAAG CTGAAAGAAG AGATTTCCAA AATGAGGGAG 
CTCCTGGCTA GAAAAGACAG CGAAACAGGA GAAAATATTA GACAGGCAGC ATCCTCTCTT 
CAGCAGGCAT CACTGAAGCT GTTCGAAATG GCATACAAAA AGATGGCATC TGAGCGAGAA 
GGCTCTGGAA GTTCTGGCAC TGGGGAACAA AAGGAAGATC AAAAGGAGGA AAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Steady-State Levels of Phosphorylated Mitogen-Activated Protein Kinase Kinase 1/2 Determined by Mortalin/HSPA9 and Protein Phosphatase 1 Alpha in
Molecular and cellular biology37(18)(2017 Sep)
Wu PK,Hong SK,Park JI

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HSPA9 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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