HLA-DRB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DRB3
Entrez Gene ID	3125
Full Name	major histocompatibility complex, class II, DR beta 3
Synonyms	HLA-DR1B,HLA-DR3B
General protein information	Preferred Names major histocompatibility complex, class II, DR beta 3 Names major histocompatibility complex, class II, DR beta 3 HLA DRB3 HLA class II histocompatibility antigen, DR beta 3 chain MHC class II HLA-DR beta 3 chain MHC class II antigen DR beta 3 chain MHC class II antigen DRB3 human leucocyte antigen DRB3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.3
Summary	HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008].
Disorder MIM:	612735

mRNA and Protein(s)

mRNA	Protein	Name
NM_022555.3	NP_072049.2	major histocompatibility complex, class II, DR beta 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

HLA-DRB1*15:01 and HLA-DRB3*02:02 in PLA2R-Related Membranous Nephropathy.
Le WB, Shi JS, Zhang T, Liu L, Qin HZ, Liang S, Zhang YW, Zheng CX, Jiang S, Qin WS, Zhang HT, Liu ZH
Journal of the American Society of Nephrology : JASN28(5)1642-1650(2017 May)

HLA-DRB3*01:01 is a predictor of immunization against human platelet antigen-1a but not of the severity of fetal and neonatal alloimmune thrombocytopenia.
Wienzek-Lischka S, K?nig IR, Papenkort EM, Hackstein H, Santoso S, Sachs UJ, Bein G
Transfusion57(3)533-540(2017 Mar)

HLA-A*33-DR3 and A*33-DR9 haplotypes enhance the risk of type 1 diabetes in Han Chinese.
Zhang J, Zhao L, Wang B, Gao J, Wang L, Li L, Cui B, Hu M, Hong J, Gu W, Wang W, Ning G
Journal of diabetes investigation7(4)514-21(2016 Jul)

Next-Generation Sequencing Reveals That HLA-DRB3, -DRB4, and -DRB5 May Be Associated With Islet Autoantibodies and Risk for Childhood Type 1 Diabetes.
Zhao LP, Alshiekh S, Zhao M, Carlsson A, Larsson HE, Forsander G, Ivarsson SA, Ludvigsson J, Kockum I, Marcus C, Persson M, Samuelsson U, ?rtqvist E, Pyo CW, Nelson WC, Geraghty DE, Lernmark ?,
Diabetes65(3)710-8(2016 Mar)

A Central Role for HLA-DR3 in Anti-Smith Antibody Responses and Glomerulonephritis in a Transgenic Mouse Model of Spontaneous Lupus.
Chowdhary VR, Dai C, Tilahun AY, Hanson JA, Smart MK, Grande JP, Rajagopalan G, Fu SM, David CS
Journal of immunology (Baltimore, Md. : 1950)195(10)4660-7(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings provide clear evidence that the HLA-DRB1*15:01 and HLA-DRB3*02:02 alleles independently and strongly associate with phospholipase A2 receptor related idiopathic membranous nephropathy in the Chinese population
Title: HLA-DRB1*15:01 and HLA-DRB3*02:02 in PLA2R-Related Membranous Nephropathy.

In contrast to HLA-DRB4*01:01P, the inheritance of HLA-DRB3*01:01 is strongly associated with the propensity for mounting a humoral immune response against fetal HPA-1a antigen.
Title: HLA-DRB3*01:01 is a predictor of immunization against human platelet antigen-1a but not of the severity of fetal and neonatal alloimmune thrombocytopenia.

The first time that the haplotypes A33-DR3 and A33-DR9 were found with an enhanced predisposition to type 1 diabetes in Han Chinese.
Title: HLA-A*33-DR3 and A*33-DR9 haplotypes enhance the risk of type 1 diabetes in Han Chinese.

HLA-DRB3 affects type 1 diabetes risk and islet autoantibodies.
Title: Next-Generation Sequencing Reveals That HLA-DRB3, -DRB4, and -DRB5 May Be Associated With Islet Autoantibodies and Risk for Childhood Type 1 Diabetes.

this mouse model unravels a critical role for HLA-DR3 in generating an autoimmune response to SmD and lupus nephritis in the NZM2328 background.
Title: A Central Role for HLA-DR3 in Anti-Smith Antibody Responses and Glomerulonephritis in a Transgenic Mouse Model of Spontaneous Lupus.

The following HLA-DRB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DRB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu03603	NM_022555.3 Latest version!	Homo sapiens major histocompatibility complex, class II, DR beta 3 (HLA-DRB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DRB3 ( NM_022555.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_072049.2 Homo sapiens major histocompatibility complex, class II, DR beta 3 (HLA-DRB3), mRNA.

Price & Availability↑

CloneID	OHu03603
Clone ID Related Accession (Same CDS sequence)	NM_022555.3
Accession Version	NM_022555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	major histocompatibility complex, class II, DR beta 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U66825.1 and Z84814.1. On or before Aug 31, 2013 this sequence version replaced XM_003846462.2, XM_002346769.1, XM_002346768.1, NM_022555.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66825.1, BC008987.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGTGTC TGAAGCTCCC TGGAGGCTCC AGCTTGGCAG CGTTGACAGT GACACTGATG 
GTGCTGAGCT CCCGACTGGC TTTCGCTGGG GACACCCGAC CACGTTTCTT GGAGCTGCGT 
AAGTCTGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTACCTGGA CAGATACTTC 
CATAACCAGG AGGAGTTCCT GCGCTTCGAC AGCGACGTGG GGGAGTACCG GGCGGTGACG 
GAGCTGGGGC GGCCTGTCGC CGAGTCCTGG AACAGCCAGA AGGACCTCCT GGAGCAGAAG 
CGGGGCCGGG TGGACAATTA CTGCAGACAC AACTACGGGG TTGGTGAGAG CTTCACAGTG 
CAGCGGCGAG TCCATCCTCA GGTGACTGTG TATCCTGCAA AGACCCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG 
TTCCGGAACG GCCAGGAAGA GAAGGCTGGG GTGGTGTCCA CGGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCCT GGTGATGCTA GAAACAGTTC CTCGGAGTGG AGAGGTTTAC 
ACTTGCCAAG TGGAGCACCC AAGCGTAACG AGCGCTCTCA CAGTGGAATG GAGAGCACGG 
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC 
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG 
CCAACAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072049.2
CDS	41..841
Translation	MVCLKLPGGSSLAALTVTLMVLSSRLAFAGDTRPRFLELRKSECHFFNGTERVRYLDRYFHNQEEFLRFDSDVGEYRAVTELGRPVAESWNSQKDLLEQKRGRVDNYCRHNYGVGESFTVQRRVHPQVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSALTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS

Target ORF information:

RefSeq Version	NM_022555.3
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DR beta 3 (HLA-DRB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022555.3

ORF Insert Sequence:

ATGGTGTGTC TGAAGCTCCC TGGAGGCTCC AGCTTGGCAG CGTTGACAGT GACACTGATG 
GTGCTGAGCT CCCGACTGGC TTTCGCTGGG GACACCCGAC CACGTTTCTT GGAGCTGCGT 
AAGTCTGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTACCTGGA CAGATACTTC 
CATAACCAGG AGGAGTTCCT GCGCTTCGAC AGCGACGTGG GGGAGTACCG GGCGGTGACG 
GAGCTGGGGC GGCCTGTCGC CGAGTCCTGG AACAGCCAGA AGGACCTCCT GGAGCAGAAG 
CGGGGCCGGG TGGACAATTA CTGCAGACAC AACTACGGGG TTGGTGAGAG CTTCACAGTG 
CAGCGGCGAG TCCATCCTCA GGTGACTGTG TATCCTGCAA AGACCCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG 
TTCCGGAACG GCCAGGAAGA GAAGGCTGGG GTGGTGTCCA CGGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCCT GGTGATGCTA GAAACAGTTC CTCGGAGTGG AGAGGTTTAC 
ACTTGCCAAG TGGAGCACCC AAGCGTAACG AGCGCTCTCA CAGTGGAATG GAGAGCACGG 
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC 
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG 
CCAACAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HLA-DRB1*15:01 and HLA-DRB3*02:02 in PLA2R-Related Membranous Nephropathy.
Journal of the American Society of Nephrology : JASN28(5)1642-1650(2017 May)
Le WB,Shi JS,Zhang T,Liu L,Qin HZ,Liang S,Zhang YW,Zheng CX,Jiang S,Qin WS,Zhang HT,Liu ZH

HLA-DRB3*01:01 is a predictor of immunization against human platelet antigen-1a but not of the severity of fetal and neonatal alloimmune thrombocytopenia.
Transfusion57(3)533-540(2017 Mar)
Wienzek-Lischka S,K?nig IR,Papenkort EM,Hackstein H,Santoso S,Sachs UJ,Bein G

HLA-A*33-DR3 and A*33-DR9 haplotypes enhance the risk of type 1 diabetes in Han Chinese.
Journal of diabetes investigation7(4)514-21(2016 Jul)
Zhang J,Zhao L,Wang B,Gao J,Wang L,Li L,Cui B,Hu M,Hong J,Gu W,Wang W,Ning G

Next-Generation Sequencing Reveals That HLA-DRB3, -DRB4, and -DRB5 May Be Associated With Islet Autoantibodies and Risk for Childhood Type 1 Diabetes.
Diabetes65(3)710-8(2016 Mar)
Zhao LP,Alshiekh S,Zhao M,Carlsson A,Larsson HE,Forsander G,Ivarsson SA,Ludvigsson J,Kockum I,Marcus C,Persson M,Samuelsson U,?rtqvist E,Pyo CW,Nelson WC,Geraghty DE,Lernmark ?,

A Central Role for HLA-DR3 in Anti-Smith Antibody Responses and Glomerulonephritis in a Transgenic Mouse Model of Spontaneous Lupus.
Journal of immunology (Baltimore, Md. : 1950)195(10)4660-7(2015 Nov)
Chowdhary VR,Dai C,Tilahun AY,Hanson JA,Smart MK,Grande JP,Rajagopalan G,Fu SM,David CS

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HLA-DRB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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