HLA-DQA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DQA2
Entrez Gene ID	3118
Full Name	major histocompatibility complex, class II, DQ alpha 2
Synonyms	DX-ALPHA,HLA-DXA,HLADQA2
General protein information	Preferred Names major histocompatibility complex, class II, DQ alpha 2 Names HLA class II histocompatibility antigen, DQ alpha 2 chain DX alpha chain HLA class II histocompatibility antigen, DQ(6) alpha chain MHC class II DQA2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	This gene belongs to the HLA class II alpha chain family. The encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells. [provided by RefSeq, Jun 2010].
Disorder MIM:	613503

mRNA and Protein(s)

mRNA	Protein	Name
NM_020056.5	NP_064440.1	HLA class II histocompatibility antigen, DQ alpha 2 chain precursor
NM_020056.4	NP_064440.1	HLA class II histocompatibility antigen, DQ alpha 2 chain precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLA-DQA2	NP_064440.1
Bos taurus (cattle)	BOLA-DQA5	NP_001012693.2
Xenopus tropicalis (tropical clawed frog)	hla-dqa1	NP_001106538.1
Pan troglodytes (chimpanzee)	PATR-DQA2	XP_527354.1
Xenopus tropicalis (tropical clawed frog)	LOC100495492	XP_002942061.2
Canis lupus familiaris (dog)	DLA-DQA1	NP_001011726.1

Gene Ontology
Bibliography

Related articles in PubMed

GWAS identifies novel SLE susceptibility genes and explains the association of the HLA region.
Armstrong DL, Zidovetzki R, Alarc?n-Riquelme ME, Tsao BP, Criswell LA, Kimberly RP, Harley JB, Sivils KL, Vyse TJ, Gaffney PM, Langefeld CD, Jacob CO
Genes and immunity15(6)347-54(2014 Sep)

Novel risk loci for rheumatoid arthritis in Han Chinese and congruence with risk variants in Europeans.
Jiang L, Yin J, Ye L, Yang J, Hemani G, Liu AJ, Zou H, He D, Sun L, Zeng X, Li Z, Zheng Y, Lin Y, Liu Y, Fang Y, Xu J, Li Y, Dai S, Guan J, Jiang L, Wei Q, Wang Y, Li Y, Huang C, Zuo X, Liu Y, Wu X, Zhang L, Zhou L, Zhang Q, Li T, Chen L, Xu Z, Yang X, Qian F, Xie W, Liu W, Guo Q, Huang S, Zhao J, Li M, Jin Y, Gao J, Lv Y, Wang Y, Lin L, Guo A, Danoy P, Willner D, Cremin C, Hadler J, Zhang F, Zhao Y, Li M, Yue T, Fan X, Guo J, Mu R, Li J, Wu C, Zeng M, Wang J, Li S, Jin L, Wang B, Wang J, Ma X, Sun L, Zhang X, Brown MA, Visscher PM, Su DF, Xu H
Arthritis & rheumatology (Hoboken, N.J.)66(5)1121-32(2014 May)

A genome-wide association study on chronic HBV infection and its clinical progression in male Han-Taiwanese.
Chang SW, Fann CS, Su WH, Wang YC, Weng CC, Yu CJ, Hsu CL, Hsieh AR, Chien RN, Chu CM, Tai DI
PloS one9(6)e99724(2014)

A genome-wide association study reveals ARL15, a novel non-HLA susceptibility gene for rheumatoid arthritis in North Indians.
Negi S, Juyal G, Senapati S, Prasad P, Gupta A, Singh S, Kashyap S, Kumar A, Kumar U, Gupta R, Kaur S, Agrawal S, Aggarwal A, Ott J, Jain S, Juyal RC, Thelma BK
Arthritis and rheumatism65(12)3026-35(2013 Dec)

Oligoclonal band status in Scandinavian multiple sclerosis patients is associated with specific genetic risk alleles.
Mero IL, Gustavsen MW, S?ther HS, Fl?m ST, Berg-Hansen P, S?ndergaard HB, Jensen PE, Berge T, Bj?lgerud A, Muggerud A, Aarseth JH, , Myhr KM, Celius EG, Sellebjerg F, Hillert J, Alfredsson L, Olsson T, Oturai AB, Kockum I, Lie BA, Andreassen BK, Harbo HF
PloS one8(3)e58352(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HLA-DQB1/HLA-DQA2 rs9275328 may play a part in influencing the systemic lupus erythematosis susceptibility in Malays and Chinese in Malaysia.
Title: HLA variants rs9271366 and rs9275328 are associated with systemic lupus erythematosus susceptibility in Malays and Chinese.

HLA-DQA2 and HLA-DQB2 genes are expressed in human Langerhans cells and encode a new HLA class II molecule.
Title: HLA-DQA2 and HLA-DQB2 genes are specifically expressed in human Langerhans cells and encode a new HLA class II molecule.

Results indicate that two SNPs rs9468925 in HLA-C/HLA-B and rs2858881 in HLA-DQA2 were repeatedly selected in all models, suggesting that multiple loci outside PSOR1 locus were associated with psoriasis.
Title: Psoriasis regression analysis of MHC loci identifies shared genetic variants with vitiligo.

Parkinson's disease-associated SNP4 is correlated (r2=0.95) with variants that are associated with HLA-DQA2 expression.
Title: Evidence for more than one Parkinson's disease-associated variant within the HLA region.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide association study identifies new HLA class II haplotypes strongly protective against narcolepsy.

The following HLA-DQA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DQA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10190	NM_020056.5 Latest version!	Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu10190	NM_020056.4	Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DQA2 ( NM_020056.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_064440.1 Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.

Price & Availability↑

CloneID	OHu10190
Clone ID Related Accession (Same CDS sequence)	NM_020056.5 , NM_020056.4
Accession Version	NM_020056.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DQ alpha 2 chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL672104.4. On May 17, 2019 this sequence version replaced NM_020056.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374940.4/ ENSP00000364076.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCCC TGACTGCCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAT GTTGCCTCCT ATGGTGTGAA CTTCTACCAG 
TCTCACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG 
GACCTGGAGA CGAAAGAGAC TGTCTGGCAG TTGCCTATGT TTAGCAAATT TATAAGTTTT 
GACCCGCAGA GTGCACTGAG AAATATGGCT GTGGGAAAAC ACACCTTGGA ATTCATGATG 
AGACAGTCCA ACTCTACCGC TGCCACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG 
TTTCCTGTGA CGCTGGGTCA GCCCAACACC CTCATCTGTC TTGTGGACAA CATCTTTCCT 
CCTGTGGTCA ACATCACCTG GCTGAGCAAT GGGCACTCAG TCACAGAAGG TGTTTCTGAG 
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC 
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CGAGCCTCTT 
CTGAAACACT GGGAGCCTGA GATTCCAGCC CCTATGTCAG AGCTCACAGA GACTTTGGTC 
TGCGCCCTGG GGTTGTCTGT GGGCCTCATG GGCATTGTGG TGGGCACTGT CTTCATCATC 
CAAGGCCTGC GTTCAGTTGG TGCTTCCAGA CACCAAGGGC TCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064440.1
CDS	54..821
Translation	MILNKALLLGALALTAVMSPCGGEDIVADHVASYGVNFYQSHGPSGQYTHEFDGDEEFYVDLETKETVWQLPMFSKFISFDPQSALRNMAVGKHTLEFMMRQSNSTAATNEVPEVTVFSKFPVTLGQPNTLICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTFLPSADEIYDCKVEHWGLDEPLLKHWEPEIPAPMSELTETLVCALGLSVGLMGIVVGTVFIIQGLRSVGASRHQGLL

Target ORF information:

RefSeq Version	NM_020056.5
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020056.5

ORF Insert Sequence:

ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCCC TGACTGCCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAT GTTGCCTCCT ATGGTGTGAA CTTCTACCAG 
TCTCACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG 
GACCTGGAGA CGAAAGAGAC TGTCTGGCAG TTGCCTATGT TTAGCAAATT TATAAGTTTT 
GACCCGCAGA GTGCACTGAG AAATATGGCT GTGGGAAAAC ACACCTTGGA ATTCATGATG 
AGACAGTCCA ACTCTACCGC TGCCACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG 
TTTCCTGTGA CGCTGGGTCA GCCCAACACC CTCATCTGTC TTGTGGACAA CATCTTTCCT 
CCTGTGGTCA ACATCACCTG GCTGAGCAAT GGGCACTCAG TCACAGAAGG TGTTTCTGAG 
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC 
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CGAGCCTCTT 
CTGAAACACT GGGAGCCTGA GATTCCAGCC CCTATGTCAG AGCTCACAGA GACTTTGGTC 
TGCGCCCTGG GGTTGTCTGT GGGCCTCATG GGCATTGTGG TGGGCACTGT CTTCATCATC 
CAAGGCCTGC GTTCAGTTGG TGCTTCCAGA CACCAAGGGC TCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLA-DQA2 ( NM_020056.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_064440.1 Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.

Price & Availability↑

CloneID	OHu10190
Clone ID Related Accession (Same CDS sequence)	NM_020056.5 , NM_020056.4
Accession Version	NM_020056.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DQ alpha 2 chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL672104.4. On or before Aug 31, 2013 this sequence version replaced XM_005275110.1, XM_005275385.1, XM_005275545.1, XM_005249050.1, XM_005272806.1, XM_005274854.1, NM_020056.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCCC TGACTGCCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAT GTTGCCTCCT ATGGTGTGAA CTTCTACCAG 
TCTCACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG 
GACCTGGAGA CGAAAGAGAC TGTCTGGCAG TTGCCTATGT TTAGCAAATT TATAAGTTTT 
GACCCGCAGA GTGCACTGAG AAATATGGCT GTGGGAAAAC ACACCTTGGA ATTCATGATG 
AGACAGTCCA ACTCTACCGC TGCCACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG 
TTTCCTGTGA CGCTGGGTCA GCCCAACACC CTCATCTGTC TTGTGGACAA CATCTTTCCT 
CCTGTGGTCA ACATCACCTG GCTGAGCAAT GGGCACTCAG TCACAGAAGG TGTTTCTGAG 
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC 
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CGAGCCTCTT 
CTGAAACACT GGGAGCCTGA GATTCCAGCC CCTATGTCAG AGCTCACAGA GACTTTGGTC 
TGCGCCCTGG GGTTGTCTGT GGGCCTCATG GGCATTGTGG TGGGCACTGT CTTCATCATC 
CAAGGCCTGC GTTCAGTTGG TGCTTCCAGA CACCAAGGGC TCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064440.1
CDS	59..826
Translation	MILNKALLLGALALTAVMSPCGGEDIVADHVASYGVNFYQSHGPSGQYTHEFDGDEEFYVDLETKETVWQLPMFSKFISFDPQSALRNMAVGKHTLEFMMRQSNSTAATNEVPEVTVFSKFPVTLGQPNTLICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTFLPSADEIYDCKVEHWGLDEPLLKHWEPEIPAPMSELTETLVCALGLSVGLMGIVVGTVFIIQGLRSVGASRHQGLL

Target ORF information:

RefSeq Version	NM_020056.4
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DQ alpha 2 (HLA-DQA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020056.4

ORF Insert Sequence:

ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCCC TGACTGCCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAT GTTGCCTCCT ATGGTGTGAA CTTCTACCAG 
TCTCACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG 
GACCTGGAGA CGAAAGAGAC TGTCTGGCAG TTGCCTATGT TTAGCAAATT TATAAGTTTT 
GACCCGCAGA GTGCACTGAG AAATATGGCT GTGGGAAAAC ACACCTTGGA ATTCATGATG 
AGACAGTCCA ACTCTACCGC TGCCACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG 
TTTCCTGTGA CGCTGGGTCA GCCCAACACC CTCATCTGTC TTGTGGACAA CATCTTTCCT 
CCTGTGGTCA ACATCACCTG GCTGAGCAAT GGGCACTCAG TCACAGAAGG TGTTTCTGAG 
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC 
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CGAGCCTCTT 
CTGAAACACT GGGAGCCTGA GATTCCAGCC CCTATGTCAG AGCTCACAGA GACTTTGGTC 
TGCGCCCTGG GGTTGTCTGT GGGCCTCATG GGCATTGTGG TGGGCACTGT CTTCATCATC 
CAAGGCCTGC GTTCAGTTGG TGCTTCCAGA CACCAAGGGC TCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GWAS identifies novel SLE susceptibility genes and explains the association of the HLA region.
Genes and immunity15(6)347-54(2014 Sep)
Armstrong DL,Zidovetzki R,Alarc?n-Riquelme ME,Tsao BP,Criswell LA,Kimberly RP,Harley JB,Sivils KL,Vyse TJ,Gaffney PM,Langefeld CD,Jacob CO

Novel risk loci for rheumatoid arthritis in Han Chinese and congruence with risk variants in Europeans.
Arthritis & rheumatology (Hoboken, N.J.)66(5)1121-32(2014 May)
Jiang L,Yin J,Ye L,Yang J,Hemani G,Liu AJ,Zou H,He D,Sun L,Zeng X,Li Z,Zheng Y,Lin Y,Liu Y,Fang Y,Xu J,Li Y,Dai S,Guan J,Jiang L,Wei Q,Wang Y,Li Y,Huang C,Zuo X,Liu Y,Wu X,Zhang L,Zhou L,Zhang Q,Li T,Chen L,Xu Z,Yang X,Qian F,Xie W,Liu W,Guo Q,Huang S,Zhao J,Li M,Jin Y,Gao J,Lv Y,Wang Y,Lin L,Guo A,Danoy P,Willner D,Cremin C,Hadler J,Zhang F,Zhao Y,Li M,Yue T,Fan X,Guo J,Mu R,Li J,Wu C,Zeng M,Wang J,Li S,Jin L,Wang B,Wang J,Ma X,Sun L,Zhang X,Brown MA,Visscher PM,Su DF,Xu H

A genome-wide association study on chronic HBV infection and its clinical progression in male Han-Taiwanese.
PloS one9(6)e99724(2014)
Chang SW,Fann CS,Su WH,Wang YC,Weng CC,Yu CJ,Hsu CL,Hsieh AR,Chien RN,Chu CM,Tai DI

A genome-wide association study reveals ARL15, a novel non-HLA susceptibility gene for rheumatoid arthritis in North Indians.
Arthritis and rheumatism65(12)3026-35(2013 Dec)
Negi S,Juyal G,Senapati S,Prasad P,Gupta A,Singh S,Kashyap S,Kumar A,Kumar U,Gupta R,Kaur S,Agrawal S,Aggarwal A,Ott J,Jain S,Juyal RC,Thelma BK

Oligoclonal band status in Scandinavian multiple sclerosis patients is associated with specific genetic risk alleles.
PloS one8(3)e58352(2013)
Mero IL,Gustavsen MW,S?ther HS,Fl?m ST,Berg-Hansen P,S?ndergaard HB,Jensen PE,Berge T,Bj?lgerud A,Muggerud A,Aarseth JH,,Myhr KM,Celius EG,Sellebjerg F,Hillert J,Alfredsson L,Olsson T,Oturai AB,Kockum I,Lie BA,Andreassen BK,Harbo HF

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HLA-DQA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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