HK1 cDNA ORF clone, Homo sapiens(human)

The following HK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09664 NM_033498.2
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu09550 NM_033496.2
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu09664 NM_001358263.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu09664 NM_001322364.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu09664 NM_033497.2
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu10118 NM_000188.2
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $69.30
$99.00
OHu44256 NM_001322366.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu09664 XM_024447969.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu44256 XM_005269737.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu19393 NM_033500.2
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu19393 XM_011539732.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu100346 NM_001322365.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100382 NM_001322367.1
Latest version!
Homo sapiens hexokinase 1 (HK1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09664
    Clone ID Related Accession (Same CDS sequence) XM_024447969.1 , NM_033498.2 , NM_001358263.1 , NM_033497.2 , NM_001322364.1
    Accession Version NM_033498.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-ta/tb
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U38226.1, AF073786.2, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced NM_033498.1. Transcript Variant: Variant 4 (HKI-tb) has four testis-specific exons in the 5' end, one of which includes an additional 54 nt fragment unique to variants 4 and 5. Variants 3, 4, 6 and 10 all encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_277033.1
    CDS359..3124
    Translation

    Target ORF information:

    RefSeq Version NM_033498.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09550
    Clone ID Related Accession (Same CDS sequence) NM_033496.2
    Accession Version NM_033496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-R
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX406321.2, BC008730.2 and AK128226.1. On May 16, 2008 this sequence version replaced NM_033496.1. Transcript Variant: Variant 2 (HKI-R) encodes the erythroid-specific isoform. Isoform HKI-R lacks the porin binding domain (PBD), which mediates association with the mitochondrial membrane, and thus localizes to the cytoplasm. Variant 2 includes an erythroid-specific exon in the 5' end which encodes a distinct N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGTG AGCACAGCCT GAGTTTGCCC TGTCGAGGTG CTGAGGCCTG GGAGATTGGG 
    ATTGACAAGT ATCTCTATGC CATGCGGCTC TCCGATGAAA CTCTCATAGA TATCATGACT
    CGCTTCAGGA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCAAC AGCCACAGTC
    AAGATGTTGC CAACATTCGT AAGGTCCATT CCTGATGGCT CTGAAAAGGG AGATTTCATT
    GCCCTGGATC TTGGTGGGTC TTCCTTTCGA ATTCTGCGGG TGCAAGTGAA TCATGAGAAA
    AACCAGAATG TTCACATGGA GTCCGAGGTT TATGACACCC CAGAGAACAT CGTGCACGGC
    AGTGGAAGCC AGCTTTTTGA TCATGTTGCT GAGTGCCTGG GAGATTTCAT GGAGAAAAGG
    AAGATCAAGG ACAAGAAGTT ACCTGTGGGA TTCACGTTTT CTTTTCCTTG CCAACAATCC
    AAAATAGATG AGGCCATCCT GATCACCTGG ACAAAGCGAT TTAAAGCGAG CGGAGTGGAA
    GGAGCAGATG TGGTCAAACT GCTTAACAAA GCCATCAAAA AGCGAGGGGA CTATGATGCC
    AACATCGTAG CTGTGGTGAA TGACACAGTG GGCACCATGA TGACCTGTGG CTATGACGAC
    CAGCACTGTG AAGTCGGCCT GATCATCGGC ACTGGCACCA ATGCTTGCTA CATGGAGGAA
    CTGAGGCACA TTGATCTGGT GGAAGGAGAC GAGGGGAGGA TGTGTATCAA TACAGAATGG
    GGAGCCTTTG GAGACGATGG ATCATTAGAA GACATCCGGA CAGAGTTTGA CAGGGAGATA
    GACCGGGGAT CCCTCAACCC TGGAAAACAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC
    TTGGGAGAGC TGGTTCGACT GATCCTAGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA
    GGGCGGATCA CCCCGGAGCT GCTCACCCGA GGGAAGTTTA ACACCAGTGA TGTGTCAGCC
    ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG
    GAGCCGTCCG ATGATGACTG TGTCTCAGTC CAGCACGTTT GCACCATTGT CTCATTTCGC
    TCAGCCAACT TGGTGGCTGC CACACTGGGC GCCATCTTGA ACCGCCTGCG TGATAACAAG
    GGCACACCCA GGCTGCGGAC CACGGTTGGT GTCGACGGAT CTCTTTACAA GACGCACCCA
    CAGTATTCCC GGCGTTTCCA CAAGACTCTA AGGCGCTTGG TGCCAGACTC CGATGTGCGC
    TTCCTCCTCT CGGAGAGTGG CAGCGGCAAG GGGGCTGCCA TGGTGACGGC GGTGGCCTAC
    CGCTTGGCCG AGCAGCACCG GCAGATAGAG GAGACCCTGG CTCATTTCCA CCTCACCAAG
    GACATGCTGC TGGAGGTGAA GAAGAGGATG CGGGCCGAGA TGGAGCTGGG GCTGAGGAAG
    CAGACGCACA ACAATGCCGT GGTTAAGATG CTGCCCTCCT TCGTCCGGAG AACTCCCGAC
    GGGACCGAGA ATGGTGACTT CTTGGCCCTG GATCTTGGAG GAACCAATTT CCGTGTGCTG
    CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA ACGGTGGAAA TGCACAACAA GATCTACGCC
    ATTCCTATTG AAATCATGCA GGGCACTGGG GAAGAGCTGT TTGATCACAT TGTCTCCTGC
    ATCTCTGACT TCTTGGACTA CATGGGGATC AAAGGCCCCA GGATGCCTCT GGGCTTCACG
    TTCTCATTTC CCTGCCAGCA GACGAGTCTG GACGCGGGAA TCTTGATCAC GTGGACAAAG
    GGTTTTAAGG CAACAGACTG CGTGGGCCAC GATGTAGTCA CCTTACTAAG GGATGCGATA
    AAAAGGAGAG AGGAATTTGA CCTGGACGTG GTGGCTGTGG TCAACGACAC AGTGGGCACC
    ATGATGACCT GTGCTTATGA GGAGCCCACC TGTGAGGTTG GACTCATTGT TGGGACCGGC
    AGCAATGCCT GCTACATGGA GGAGATGAAG AACGTGGAGA TGGTGGAGGG GGACCAGGGG
    CAGATGTGCA TCAACATGGA GTGGGGGGCC TTTGGGGACA ACGGGTGTCT GGATGATATC
    AGGACACACT ACGACAGACT GGTGGACGAA TATTCCCTAA ATGCTGGGAA ACAAAGGTAT
    GAGAAGATGA TCAGTGGTAT GTACCTGGGT GAAATCGTCC GCAACATCTT AATCGACTTC
    ACCAAGAAGG GATTCCTCTT CCGAGGGCAG ATCTCTGAGA CGCTGAAGAC CCGGGGCATC
    TTTGAGACCA AGTTTCTCTC TCAGATCGAG AGTGACCGAT TAGCACTGCT CCAGGTCCGG
    GCTATCCTCC AGCAGCTAGG TCTGAATAGC ACCTGCGATG ACAGTATCCT CGTCAAGACA
    GTGTGCGGGG TGGTGTCCAG GAGGGCCGCA CAGCTGTGTG GCGCAGGCAT GGCTGCGGTT
    GTGGATAAGA TCCGCGAGAA CAGAGGACTG GACCGTCTGA ATGTGACTGT GGGAGTGGAC
    GGGACACTCT ACAAGCTTCA TCCACACTTC TCCAGAATCA TGCACCAGAC GGTGAAGGAA
    CTGTCACCAA AATGTAACGT GTCCTTCCTC CTGTCTGAGG ATGGCAGCGG CAAGGGGGCC
    GCCCTCATCA CGGCCGTGGG CGTGCGGTTA CGCACAGAGG CAAGCAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_277031.1
    CDS102..2852
    Translation

    Target ORF information:

    RefSeq Version NM_033496.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGTG AGCACAGCCT GAGTTTGCCC TGTCGAGGTG CTGAGGCCTG GGAGATTGGG 
    ATTGACAAGT ATCTCTATGC CATGCGGCTC TCCGATGAAA CTCTCATAGA TATCATGACT
    CGCTTCAGGA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCAAC AGCCACAGTC
    AAGATGTTGC CAACATTCGT AAGGTCCATT CCTGATGGCT CTGAAAAGGG AGATTTCATT
    GCCCTGGATC TTGGTGGGTC TTCCTTTCGA ATTCTGCGGG TGCAAGTGAA TCATGAGAAA
    AACCAGAATG TTCACATGGA GTCCGAGGTT TATGACACCC CAGAGAACAT CGTGCACGGC
    AGTGGAAGCC AGCTTTTTGA TCATGTTGCT GAGTGCCTGG GAGATTTCAT GGAGAAAAGG
    AAGATCAAGG ACAAGAAGTT ACCTGTGGGA TTCACGTTTT CTTTTCCTTG CCAACAATCC
    AAAATAGATG AGGCCATCCT GATCACCTGG ACAAAGCGAT TTAAAGCGAG CGGAGTGGAA
    GGAGCAGATG TGGTCAAACT GCTTAACAAA GCCATCAAAA AGCGAGGGGA CTATGATGCC
    AACATCGTAG CTGTGGTGAA TGACACAGTG GGCACCATGA TGACCTGTGG CTATGACGAC
    CAGCACTGTG AAGTCGGCCT GATCATCGGC ACTGGCACCA ATGCTTGCTA CATGGAGGAA
    CTGAGGCACA TTGATCTGGT GGAAGGAGAC GAGGGGAGGA TGTGTATCAA TACAGAATGG
    GGAGCCTTTG GAGACGATGG ATCATTAGAA GACATCCGGA CAGAGTTTGA CAGGGAGATA
    GACCGGGGAT CCCTCAACCC TGGAAAACAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC
    TTGGGAGAGC TGGTTCGACT GATCCTAGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA
    GGGCGGATCA CCCCGGAGCT GCTCACCCGA GGGAAGTTTA ACACCAGTGA TGTGTCAGCC
    ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG
    GAGCCGTCCG ATGATGACTG TGTCTCAGTC CAGCACGTTT GCACCATTGT CTCATTTCGC
    TCAGCCAACT TGGTGGCTGC CACACTGGGC GCCATCTTGA ACCGCCTGCG TGATAACAAG
    GGCACACCCA GGCTGCGGAC CACGGTTGGT GTCGACGGAT CTCTTTACAA GACGCACCCA
    CAGTATTCCC GGCGTTTCCA CAAGACTCTA AGGCGCTTGG TGCCAGACTC CGATGTGCGC
    TTCCTCCTCT CGGAGAGTGG CAGCGGCAAG GGGGCTGCCA TGGTGACGGC GGTGGCCTAC
    CGCTTGGCCG AGCAGCACCG GCAGATAGAG GAGACCCTGG CTCATTTCCA CCTCACCAAG
    GACATGCTGC TGGAGGTGAA GAAGAGGATG CGGGCCGAGA TGGAGCTGGG GCTGAGGAAG
    CAGACGCACA ACAATGCCGT GGTTAAGATG CTGCCCTCCT TCGTCCGGAG AACTCCCGAC
    GGGACCGAGA ATGGTGACTT CTTGGCCCTG GATCTTGGAG GAACCAATTT CCGTGTGCTG
    CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA ACGGTGGAAA TGCACAACAA GATCTACGCC
    ATTCCTATTG AAATCATGCA GGGCACTGGG GAAGAGCTGT TTGATCACAT TGTCTCCTGC
    ATCTCTGACT TCTTGGACTA CATGGGGATC AAAGGCCCCA GGATGCCTCT GGGCTTCACG
    TTCTCATTTC CCTGCCAGCA GACGAGTCTG GACGCGGGAA TCTTGATCAC GTGGACAAAG
    GGTTTTAAGG CAACAGACTG CGTGGGCCAC GATGTAGTCA CCTTACTAAG GGATGCGATA
    AAAAGGAGAG AGGAATTTGA CCTGGACGTG GTGGCTGTGG TCAACGACAC AGTGGGCACC
    ATGATGACCT GTGCTTATGA GGAGCCCACC TGTGAGGTTG GACTCATTGT TGGGACCGGC
    AGCAATGCCT GCTACATGGA GGAGATGAAG AACGTGGAGA TGGTGGAGGG GGACCAGGGG
    CAGATGTGCA TCAACATGGA GTGGGGGGCC TTTGGGGACA ACGGGTGTCT GGATGATATC
    AGGACACACT ACGACAGACT GGTGGACGAA TATTCCCTAA ATGCTGGGAA ACAAAGGTAT
    GAGAAGATGA TCAGTGGTAT GTACCTGGGT GAAATCGTCC GCAACATCTT AATCGACTTC
    ACCAAGAAGG GATTCCTCTT CCGAGGGCAG ATCTCTGAGA CGCTGAAGAC CCGGGGCATC
    TTTGAGACCA AGTTTCTCTC TCAGATCGAG AGTGACCGAT TAGCACTGCT CCAGGTCCGG
    GCTATCCTCC AGCAGCTAGG TCTGAATAGC ACCTGCGATG ACAGTATCCT CGTCAAGACA
    GTGTGCGGGG TGGTGTCCAG GAGGGCCGCA CAGCTGTGTG GCGCAGGCAT GGCTGCGGTT
    GTGGATAAGA TCCGCGAGAA CAGAGGACTG GACCGTCTGA ATGTGACTGT GGGAGTGGAC
    GGGACACTCT ACAAGCTTCA TCCACACTTC TCCAGAATCA TGCACCAGAC GGTGAAGGAA
    CTGTCACCAA AATGTAACGT GTCCTTCCTC CTGTCTGAGG ATGGCAGCGG CAAGGGGGCC
    GCCCTCATCA CGGCCGTGGG CGTGCGGTTA CGCACAGAGG CAAGCAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09664
    Clone ID Related Accession (Same CDS sequence) XM_024447969.1 , NM_033498.2 , NM_001358263.1 , NM_033497.2 , NM_001322364.1
    Accession Version NM_001358263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-ta/tb
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596223.3, BM686492.1 and AC016821.9. Transcript Variant: Variants 3, 4, 6 and 10 all encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660805.13541.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345192.1
    CDS486..3251
    Translation

    Target ORF information:

    RefSeq Version NM_001358263.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09664
    Clone ID Related Accession (Same CDS sequence) XM_024447969.1 , NM_033498.2 , NM_001358263.1 , NM_033497.2 , NM_001322364.1
    Accession Version NM_033497.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-ta/tb
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB088955.1, AF073786.2, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced NM_033497.1. Transcript Variant: Variant 3 (HKI-ta) includes four testis-specific exons in the 5' end. Variants 3, 4, 6 and 10 all encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_277032.1
    CDS305..3070
    Translation

    Target ORF information:

    RefSeq Version NM_033497.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09664
    Clone ID Related Accession (Same CDS sequence) XM_024447969.1 , NM_033498.2 , NM_001358263.1 , NM_033497.2 , NM_001322364.1
    Accession Version NM_001322364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-ta/tb
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596223.3 and AC016821.9. On Apr 12, 2016 this sequence version replaced XM_005269736.1. Transcript Variant: Variants 3, 4, 6 and 10 all encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309293.1
    CDS183..2948
    Translation

    Target ORF information:

    RefSeq Version NM_001322364.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001322364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10118
    Clone ID Related Accession (Same CDS sequence) NM_000188.2
    Accession Version NM_000188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292012.1, AB209526.1, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced NM_000188.1. Transcript Variant: Variant 1 (HKI) encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.126948.1, SRR1660809.35161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
    AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
    ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
    GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
    ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
    AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
    GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
    AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
    TCCAAAATAG ATGAGGCCAT CCTGATCACC TGGACAAAGC GATTTAAAGC GAGCGGAGTG
    GAAGGAGCAG ATGTGGTCAA ACTGCTTAAC AAAGCCATCA AAAAGCGAGG GGACTATGAT
    GCCAACATCG TAGCTGTGGT GAATGACACA GTGGGCACCA TGATGACCTG TGGCTATGAC
    GACCAGCACT GTGAAGTCGG CCTGATCATC GGCACTGGCA CCAATGCTTG CTACATGGAG
    GAACTGAGGC ACATTGATCT GGTGGAAGGA GACGAGGGGA GGATGTGTAT CAATACAGAA
    TGGGGAGCCT TTGGAGACGA TGGATCATTA GAAGACATCC GGACAGAGTT TGACAGGGAG
    ATAGACCGGG GATCCCTCAA CCCTGGAAAA CAGCTGTTTG AGAAGATGGT CAGTGGCATG
    TACTTGGGAG AGCTGGTTCG ACTGATCCTA GTCAAGATGG CCAAGGAGGG CCTCTTATTT
    GAAGGGCGGA TCACCCCGGA GCTGCTCACC CGAGGGAAGT TTAACACCAG TGATGTGTCA
    GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA
    GTGGAGCCGT CCGATGATGA CTGTGTCTCA GTCCAGCACG TTTGCACCAT TGTCTCATTT
    CGCTCAGCCA ACTTGGTGGC TGCCACACTG GGCGCCATCT TGAACCGCCT GCGTGATAAC
    AAGGGCACAC CCAGGCTGCG GACCACGGTT GGTGTCGACG GATCTCTTTA CAAGACGCAC
    CCACAGTATT CCCGGCGTTT CCACAAGACT CTAAGGCGCT TGGTGCCAGA CTCCGATGTG
    CGCTTCCTCC TCTCGGAGAG TGGCAGCGGC AAGGGGGCTG CCATGGTGAC GGCGGTGGCC
    TACCGCTTGG CCGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCTCATTT CCACCTCACC
    AAGGACATGC TGCTGGAGGT GAAGAAGAGG ATGCGGGCCG AGATGGAGCT GGGGCTGAGG
    AAGCAGACGC ACAACAATGC CGTGGTTAAG ATGCTGCCCT CCTTCGTCCG GAGAACTCCC
    GACGGGACCG AGAATGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACCAA TTTCCGTGTG
    CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGAACGGTGG AAATGCACAA CAAGATCTAC
    GCCATTCCTA TTGAAATCAT GCAGGGCACT GGGGAAGAGC TGTTTGATCA CATTGTCTCC
    TGCATCTCTG ACTTCTTGGA CTACATGGGG ATCAAAGGCC CCAGGATGCC TCTGGGCTTC
    ACGTTCTCAT TTCCCTGCCA GCAGACGAGT CTGGACGCGG GAATCTTGAT CACGTGGACA
    AAGGGTTTTA AGGCAACAGA CTGCGTGGGC CACGATGTAG TCACCTTACT AAGGGATGCG
    ATAAAAAGGA GAGAGGAATT TGACCTGGAC GTGGTGGCTG TGGTCAACGA CACAGTGGGC
    ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGTGAGG TTGGACTCAT TGTTGGGACC
    GGCAGCAATG CCTGCTACAT GGAGGAGATG AAGAACGTGG AGATGGTGGA GGGGGACCAG
    GGGCAGATGT GCATCAACAT GGAGTGGGGG GCCTTTGGGG ACAACGGGTG TCTGGATGAT
    ATCAGGACAC ACTACGACAG ACTGGTGGAC GAATATTCCC TAAATGCTGG GAAACAAAGG
    TATGAGAAGA TGATCAGTGG TATGTACCTG GGTGAAATCG TCCGCAACAT CTTAATCGAC
    TTCACCAAGA AGGGATTCCT CTTCCGAGGG CAGATCTCTG AGACGCTGAA GACCCGGGGC
    ATCTTTGAGA CCAAGTTTCT CTCTCAGATC GAGAGTGACC GATTAGCACT GCTCCAGGTC
    CGGGCTATCC TCCAGCAGCT AGGTCTGAAT AGCACCTGCG ATGACAGTAT CCTCGTCAAG
    ACAGTGTGCG GGGTGGTGTC CAGGAGGGCC GCACAGCTGT GTGGCGCAGG CATGGCTGCG
    GTTGTGGATA AGATCCGCGA GAACAGAGGA CTGGACCGTC TGAATGTGAC TGTGGGAGTG
    GACGGGACAC TCTACAAGCT TCATCCACAC TTCTCCAGAA TCATGCACCA GACGGTGAAG
    GAACTGTCAC CAAAATGTAA CGTGTCCTTC CTCCTGTCTG AGGATGGCAG CGGCAAGGGG
    GCCGCCCTCA TCACGGCCGT GGGCGTGCGG TTACGCACAG AGGCAAGCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000179.2
    CDS102..2855
    Translation

    Target ORF information:

    RefSeq Version NM_000188.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
    AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
    ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
    GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
    ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
    AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
    GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
    AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
    TCCAAAATAG ATGAGGCCAT CCTGATCACC TGGACAAAGC GATTTAAAGC GAGCGGAGTG
    GAAGGAGCAG ATGTGGTCAA ACTGCTTAAC AAAGCCATCA AAAAGCGAGG GGACTATGAT
    GCCAACATCG TAGCTGTGGT GAATGACACA GTGGGCACCA TGATGACCTG TGGCTATGAC
    GACCAGCACT GTGAAGTCGG CCTGATCATC GGCACTGGCA CCAATGCTTG CTACATGGAG
    GAACTGAGGC ACATTGATCT GGTGGAAGGA GACGAGGGGA GGATGTGTAT CAATACAGAA
    TGGGGAGCCT TTGGAGACGA TGGATCATTA GAAGACATCC GGACAGAGTT TGACAGGGAG
    ATAGACCGGG GATCCCTCAA CCCTGGAAAA CAGCTGTTTG AGAAGATGGT CAGTGGCATG
    TACTTGGGAG AGCTGGTTCG ACTGATCCTA GTCAAGATGG CCAAGGAGGG CCTCTTATTT
    GAAGGGCGGA TCACCCCGGA GCTGCTCACC CGAGGGAAGT TTAACACCAG TGATGTGTCA
    GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA
    GTGGAGCCGT CCGATGATGA CTGTGTCTCA GTCCAGCACG TTTGCACCAT TGTCTCATTT
    CGCTCAGCCA ACTTGGTGGC TGCCACACTG GGCGCCATCT TGAACCGCCT GCGTGATAAC
    AAGGGCACAC CCAGGCTGCG GACCACGGTT GGTGTCGACG GATCTCTTTA CAAGACGCAC
    CCACAGTATT CCCGGCGTTT CCACAAGACT CTAAGGCGCT TGGTGCCAGA CTCCGATGTG
    CGCTTCCTCC TCTCGGAGAG TGGCAGCGGC AAGGGGGCTG CCATGGTGAC GGCGGTGGCC
    TACCGCTTGG CCGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCTCATTT CCACCTCACC
    AAGGACATGC TGCTGGAGGT GAAGAAGAGG ATGCGGGCCG AGATGGAGCT GGGGCTGAGG
    AAGCAGACGC ACAACAATGC CGTGGTTAAG ATGCTGCCCT CCTTCGTCCG GAGAACTCCC
    GACGGGACCG AGAATGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACCAA TTTCCGTGTG
    CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGAACGGTGG AAATGCACAA CAAGATCTAC
    GCCATTCCTA TTGAAATCAT GCAGGGCACT GGGGAAGAGC TGTTTGATCA CATTGTCTCC
    TGCATCTCTG ACTTCTTGGA CTACATGGGG ATCAAAGGCC CCAGGATGCC TCTGGGCTTC
    ACGTTCTCAT TTCCCTGCCA GCAGACGAGT CTGGACGCGG GAATCTTGAT CACGTGGACA
    AAGGGTTTTA AGGCAACAGA CTGCGTGGGC CACGATGTAG TCACCTTACT AAGGGATGCG
    ATAAAAAGGA GAGAGGAATT TGACCTGGAC GTGGTGGCTG TGGTCAACGA CACAGTGGGC
    ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGTGAGG TTGGACTCAT TGTTGGGACC
    GGCAGCAATG CCTGCTACAT GGAGGAGATG AAGAACGTGG AGATGGTGGA GGGGGACCAG
    GGGCAGATGT GCATCAACAT GGAGTGGGGG GCCTTTGGGG ACAACGGGTG TCTGGATGAT
    ATCAGGACAC ACTACGACAG ACTGGTGGAC GAATATTCCC TAAATGCTGG GAAACAAAGG
    TATGAGAAGA TGATCAGTGG TATGTACCTG GGTGAAATCG TCCGCAACAT CTTAATCGAC
    TTCACCAAGA AGGGATTCCT CTTCCGAGGG CAGATCTCTG AGACGCTGAA GACCCGGGGC
    ATCTTTGAGA CCAAGTTTCT CTCTCAGATC GAGAGTGACC GATTAGCACT GCTCCAGGTC
    CGGGCTATCC TCCAGCAGCT AGGTCTGAAT AGCACCTGCG ATGACAGTAT CCTCGTCAAG
    ACAGTGTGCG GGGTGGTGTC CAGGAGGGCC GCACAGCTGT GTGGCGCAGG CATGGCTGCG
    GTTGTGGATA AGATCCGCGA GAACAGAGGA CTGGACCGTC TGAATGTGAC TGTGGGAGTG
    GACGGGACAC TCTACAAGCT TCATCCACAC TTCTCCAGAA TCATGCACCA GACGGTGAAG
    GAACTGTCAC CAAAATGTAA CGTGTCCTTC CTCCTGTCTG AGGATGGCAG CGGCAAGGGG
    GCCGCCCTCA TCACGGCCGT GGGCGTGCGG TTACGCACAG AGGCAAGCAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44256
    Clone ID Related Accession (Same CDS sequence) XM_005269737.1 , NM_001322366.1
    Accession Version NM_001322366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596223.3 and AC016821.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.171811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
    AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
    AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
    TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
    TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
    CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
    CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
    ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
    CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
    GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
    ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
    GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
    TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
    GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
    ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
    CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
    CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
    GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
    ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
    ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
    AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
    AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
    CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
    AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
    GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
    TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
    AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
    GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
    ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
    ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
    GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
    CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
    GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
    GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
    TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
    GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
    TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
    CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
    CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
    CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
    AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
    AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
    CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
    TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
    GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309295.1
    CDS77..2746
    Translation

    Target ORF information:

    RefSeq Version NM_001322366.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001322366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
    AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
    AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
    TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
    TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
    CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
    CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
    ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
    CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
    GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
    ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
    GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
    TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
    GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
    ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
    CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
    CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
    GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
    ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
    ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
    AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
    AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
    CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
    AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
    GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
    TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
    AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
    GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
    ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
    ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
    GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
    CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
    GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
    GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
    TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
    GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
    TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
    CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
    CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
    CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
    AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
    AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
    CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
    TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
    GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09664
    Clone ID Related Accession (Same CDS sequence) XM_024447969.1 , NM_033498.2 , NM_001358263.1 , NM_033497.2 , NM_001322364.1
    Accession Version XM_024447969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024303737.1
    CDS549..3314
    Translation

    Target ORF information:

    RefSeq Version XM_024447969.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024447969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
    CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
    ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
    CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
    AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
    GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
    AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
    TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
    CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
    GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
    GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
    TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
    TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
    ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
    TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
    GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
    GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
    AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
    ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
    ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
    CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
    TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
    GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
    ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
    TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
    CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
    CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
    AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
    AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
    CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
    CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
    ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
    CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
    GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
    ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
    GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
    TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
    GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
    ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
    AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
    CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
    ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
    GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
    ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
    CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
    AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44256
    Clone ID Related Accession (Same CDS sequence) XM_005269737.1 , NM_001322366.1
    Accession Version XM_005269737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
    AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
    AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
    TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
    TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
    CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
    CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
    ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
    CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
    GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
    ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
    GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
    TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
    GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
    ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
    CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
    CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
    GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
    ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
    ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
    AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
    AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
    CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
    AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
    GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
    TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
    AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
    GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
    ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
    ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
    GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
    CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
    GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
    GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
    TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
    GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
    TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
    CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
    CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
    CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
    AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
    AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
    CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
    TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
    GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005269794.1
    CDS457..3126
    Translation

    Target ORF information:

    RefSeq Version XM_005269737.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005269737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
    AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
    AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
    TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
    TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
    CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
    CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
    ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
    CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
    GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
    ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
    GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
    TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
    GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
    ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
    CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
    CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
    GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
    ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
    ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
    AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
    AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
    CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
    AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
    GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
    TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
    AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
    GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
    ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
    ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
    GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
    CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
    GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
    GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
    TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
    GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
    TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
    CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
    CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
    CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
    AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
    AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
    CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
    TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
    GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19393
    Clone ID Related Accession (Same CDS sequence) NM_033500.2 , XM_011539732.1
    Accession Version NM_033500.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform HKI-td
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U38227.1, AF073786.2, AK128226.1 and BC008730.2. This sequence is a reference standard in the RefSeqGene project. On May 16, 2008 this sequence version replaced NM_033500.1. Transcript Variant: Variant 5 (HKI-td) has five testis-specific exons in the 5' end, one of which includes an additional 54 nt fragment unique to variants 4 and 5. Isoform HKI-td has a unique N-terminus and lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta inferred exon combination :: PMID: 10978502 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
    GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
    CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
    GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
    CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
    GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
    TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
    ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
    AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
    ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_277035.2
    CDS500..3217
    Translation

    Target ORF information:

    RefSeq Version NM_033500.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
    GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
    CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
    GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
    CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
    GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
    TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
    ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
    AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
    ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19393
    Clone ID Related Accession (Same CDS sequence) NM_033500.2 , XM_011539732.1
    Accession Version XM_011539732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
    GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
    CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
    GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
    CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
    GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
    TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
    ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
    AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
    ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538034.1
    CDS407..3124
    Translation

    Target ORF information:

    RefSeq Version XM_011539732.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011539732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
    GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
    CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
    GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
    CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
    GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
    TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
    ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
    AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
    ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100346
    Clone ID Related Accession (Same CDS sequence) NM_001322365.1
    Accession Version NM_001322365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596223.3 and AC016821.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128226.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGCAGA TCTGCCAGCG AGAATCGAGA CAGGGTCTTG CTATGTTGCC CAGGCTGTTC 
    TTAAATCCCT GGCGTCAAGT GATCCTCCTG CCTCAGCCTC CTAAAGTGCT GGGATTACAG
    GCTACAGCAG CTGAAAAACC AAAACTTCAT CTACTTGCTG AAAGTGAGAT TGACAAGTAT
    CTCTATGCCA TGCGGCTCTC CGATGAAACT CTCATAGATA TCATGACTCG CTTCAGGAAG
    GAGATGAAGA ATGGCCTCTC CCGGGATTTT AATCCAACAG CCACAGTCAA GATGTTGCCA
    ACATTCGTAA GGTCCATTCC TGATGGCTCT GAAAAGGGAG ATTTCATTGC CCTGGATCTT
    GGTGGGTCTT CCTTTCGAAT TCTGCGGGTG CAAGTGAATC ATGAGAAAAA CCAGAATGTT
    CACATGGAGT CCGAGGTTTA TGACACCCCA GAGAACATCG TGCACGGCAG TGGAAGCCAG
    CTTTTTGATC ATGTTGCTGA GTGCCTGGGA GATTTCATGG AGAAAAGGAA GATCAAGGAC
    AAGAAGTTAC CTGTGGGATT CACGTTTTCT TTTCCTTGCC AACAATCCAA AATAGATGAG
    GCCATCCTGA TCACCTGGAC AAAGCGATTT AAAGCGAGCG GAGTGGAAGG AGCAGATGTG
    GTCAAACTGC TTAACAAAGC CATCAAAAAG CGAGGGGACT ATGATGCCAA CATCGTAGCT
    GTGGTGAATG ACACAGTGGG CACCATGATG ACCTGTGGCT ATGACGACCA GCACTGTGAA
    GTCGGCCTGA TCATCGGCAC TGGCACCAAT GCTTGCTACA TGGAGGAACT GAGGCACATT
    GATCTGGTGG AAGGAGACGA GGGGAGGATG TGTATCAATA CAGAATGGGG AGCCTTTGGA
    GACGATGGAT CATTAGAAGA CATCCGGACA GAGTTTGACA GGGAGATAGA CCGGGGATCC
    CTCAACCCTG GAAAACAGCT GTTTGAGAAG ATGGTCAGTG GCATGTACTT GGGAGAGCTG
    GTTCGACTGA TCCTAGTCAA GATGGCCAAG GAGGGCCTCT TATTTGAAGG GCGGATCACC
    CCGGAGCTGC TCACCCGAGG GAAGTTTAAC ACCAGTGATG TGTCAGCCAT CGAAAAGAAT
    AAGGAAGGCC TCCACAATGC CAAAGAAATC CTGACCCGCC TGGGAGTGGA GCCGTCCGAT
    GATGACTGTG TCTCAGTCCA GCACGTTTGC ACCATTGTCT CATTTCGCTC AGCCAACTTG
    GTGGCTGCCA CACTGGGCGC CATCTTGAAC CGCCTGCGTG ATAACAAGGG CACACCCAGG
    CTGCGGACCA CGGTTGGTGT CGACGGATCT CTTTACAAGA CGCACCCACA GTATTCCCGG
    CGTTTCCACA AGACTCTAAG GCGCTTGGTG CCAGACTCCG ATGTGCGCTT CCTCCTCTCG
    GAGAGTGGCA GCGGCAAGGG GGCTGCCATG GTGACGGCGG TGGCCTACCG CTTGGCCGAG
    CAGCACCGGC AGATAGAGGA GACCCTGGCT CATTTCCACC TCACCAAGGA CATGCTGCTG
    GAGGTGAAGA AGAGGATGCG GGCCGAGATG GAGCTGGGGC TGAGGAAGCA GACGCACAAC
    AATGCCGTGG TTAAGATGCT GCCCTCCTTC GTCCGGAGAA CTCCCGACGG GACCGAGAAT
    GGTGACTTCT TGGCCCTGGA TCTTGGAGGA ACCAATTTCC GTGTGCTGCT GGTGAAAATC
    CGTAGTGGGA AAAAGAGAAC GGTGGAAATG CACAACAAGA TCTACGCCAT TCCTATTGAA
    ATCATGCAGG GCACTGGGGA AGAGCTGTTT GATCACATTG TCTCCTGCAT CTCTGACTTC
    TTGGACTACA TGGGGATCAA AGGCCCCAGG ATGCCTCTGG GCTTCACGTT CTCATTTCCC
    TGCCAGCAGA CGAGTCTGGA CGCGGGAATC TTGATCACGT GGACAAAGGG TTTTAAGGCA
    ACAGACTGCG TGGGCCACGA TGTAGTCACC TTACTAAGGG ATGCGATAAA AAGGAGAGAG
    GAATTTGACC TGGACGTGGT GGCTGTGGTC AACGACACAG TGGGCACCAT GATGACCTGT
    GCTTATGAGG AGCCCACCTG TGAGGTTGGA CTCATTGTTG GGACCGGCAG CAATGCCTGC
    TACATGGAGG AGATGAAGAA CGTGGAGATG GTGGAGGGGG ACCAGGGGCA GATGTGCATC
    AACATGGAGT GGGGGGCCTT TGGGGACAAC GGGTGTCTGG ATGATATCAG GACACACTAC
    GACAGACTGG TGGACGAATA TTCCCTAAAT GCTGGGAAAC AAAGGTATGA GAAGATGATC
    AGTGGTATGT ACCTGGGTGA AATCGTCCGC AACATCTTAA TCGACTTCAC CAAGAAGGGA
    TTCCTCTTCC GAGGGCAGAT CTCTGAGACG CTGAAGACCC GGGGCATCTT TGAGACCAAG
    TTTCTCTCTC AGATCGAGAG TGACCGATTA GCACTGCTCC AGGTCCGGGC TATCCTCCAG
    CAGCTAGGTC TGAATAGCAC CTGCGATGAC AGTATCCTCG TCAAGACAGT GTGCGGGGTG
    GTGTCCAGGA GGGCCGCACA GCTGTGTGGC GCAGGCATGG CTGCGGTTGT GGATAAGATC
    CGCGAGAACA GAGGACTGGA CCGTCTGAAT GTGACTGTGG GAGTGGACGG GACACTCTAC
    AAGCTTCATC CACACTTCTC CAGAATCATG CACCAGACGG TGAAGGAACT GTCACCAAAA
    TGTAACGTGT CCTTCCTCCT GTCTGAGGAT GGCAGCGGCA AGGGGGCCGC CCTCATCACG
    GCCGTGGGCG TGCGGTTACG CACAGAGGCA AGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309294.1
    CDS395..3253
    Translation

    Target ORF information:

    RefSeq Version NM_001322365.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001322365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGCAGA TCTGCCAGCG AGAATCGAGA CAGGGTCTTG CTATGTTGCC CAGGCTGTTC 
    TTAAATCCCT GGCGTCAAGT GATCCTCCTG CCTCAGCCTC CTAAAGTGCT GGGATTACAG
    GCTACAGCAG CTGAAAAACC AAAACTTCAT CTACTTGCTG AAAGTGAGAT TGACAAGTAT
    CTCTATGCCA TGCGGCTCTC CGATGAAACT CTCATAGATA TCATGACTCG CTTCAGGAAG
    GAGATGAAGA ATGGCCTCTC CCGGGATTTT AATCCAACAG CCACAGTCAA GATGTTGCCA
    ACATTCGTAA GGTCCATTCC TGATGGCTCT GAAAAGGGAG ATTTCATTGC CCTGGATCTT
    GGTGGGTCTT CCTTTCGAAT TCTGCGGGTG CAAGTGAATC ATGAGAAAAA CCAGAATGTT
    CACATGGAGT CCGAGGTTTA TGACACCCCA GAGAACATCG TGCACGGCAG TGGAAGCCAG
    CTTTTTGATC ATGTTGCTGA GTGCCTGGGA GATTTCATGG AGAAAAGGAA GATCAAGGAC
    AAGAAGTTAC CTGTGGGATT CACGTTTTCT TTTCCTTGCC AACAATCCAA AATAGATGAG
    GCCATCCTGA TCACCTGGAC AAAGCGATTT AAAGCGAGCG GAGTGGAAGG AGCAGATGTG
    GTCAAACTGC TTAACAAAGC CATCAAAAAG CGAGGGGACT ATGATGCCAA CATCGTAGCT
    GTGGTGAATG ACACAGTGGG CACCATGATG ACCTGTGGCT ATGACGACCA GCACTGTGAA
    GTCGGCCTGA TCATCGGCAC TGGCACCAAT GCTTGCTACA TGGAGGAACT GAGGCACATT
    GATCTGGTGG AAGGAGACGA GGGGAGGATG TGTATCAATA CAGAATGGGG AGCCTTTGGA
    GACGATGGAT CATTAGAAGA CATCCGGACA GAGTTTGACA GGGAGATAGA CCGGGGATCC
    CTCAACCCTG GAAAACAGCT GTTTGAGAAG ATGGTCAGTG GCATGTACTT GGGAGAGCTG
    GTTCGACTGA TCCTAGTCAA GATGGCCAAG GAGGGCCTCT TATTTGAAGG GCGGATCACC
    CCGGAGCTGC TCACCCGAGG GAAGTTTAAC ACCAGTGATG TGTCAGCCAT CGAAAAGAAT
    AAGGAAGGCC TCCACAATGC CAAAGAAATC CTGACCCGCC TGGGAGTGGA GCCGTCCGAT
    GATGACTGTG TCTCAGTCCA GCACGTTTGC ACCATTGTCT CATTTCGCTC AGCCAACTTG
    GTGGCTGCCA CACTGGGCGC CATCTTGAAC CGCCTGCGTG ATAACAAGGG CACACCCAGG
    CTGCGGACCA CGGTTGGTGT CGACGGATCT CTTTACAAGA CGCACCCACA GTATTCCCGG
    CGTTTCCACA AGACTCTAAG GCGCTTGGTG CCAGACTCCG ATGTGCGCTT CCTCCTCTCG
    GAGAGTGGCA GCGGCAAGGG GGCTGCCATG GTGACGGCGG TGGCCTACCG CTTGGCCGAG
    CAGCACCGGC AGATAGAGGA GACCCTGGCT CATTTCCACC TCACCAAGGA CATGCTGCTG
    GAGGTGAAGA AGAGGATGCG GGCCGAGATG GAGCTGGGGC TGAGGAAGCA GACGCACAAC
    AATGCCGTGG TTAAGATGCT GCCCTCCTTC GTCCGGAGAA CTCCCGACGG GACCGAGAAT
    GGTGACTTCT TGGCCCTGGA TCTTGGAGGA ACCAATTTCC GTGTGCTGCT GGTGAAAATC
    CGTAGTGGGA AAAAGAGAAC GGTGGAAATG CACAACAAGA TCTACGCCAT TCCTATTGAA
    ATCATGCAGG GCACTGGGGA AGAGCTGTTT GATCACATTG TCTCCTGCAT CTCTGACTTC
    TTGGACTACA TGGGGATCAA AGGCCCCAGG ATGCCTCTGG GCTTCACGTT CTCATTTCCC
    TGCCAGCAGA CGAGTCTGGA CGCGGGAATC TTGATCACGT GGACAAAGGG TTTTAAGGCA
    ACAGACTGCG TGGGCCACGA TGTAGTCACC TTACTAAGGG ATGCGATAAA AAGGAGAGAG
    GAATTTGACC TGGACGTGGT GGCTGTGGTC AACGACACAG TGGGCACCAT GATGACCTGT
    GCTTATGAGG AGCCCACCTG TGAGGTTGGA CTCATTGTTG GGACCGGCAG CAATGCCTGC
    TACATGGAGG AGATGAAGAA CGTGGAGATG GTGGAGGGGG ACCAGGGGCA GATGTGCATC
    AACATGGAGT GGGGGGCCTT TGGGGACAAC GGGTGTCTGG ATGATATCAG GACACACTAC
    GACAGACTGG TGGACGAATA TTCCCTAAAT GCTGGGAAAC AAAGGTATGA GAAGATGATC
    AGTGGTATGT ACCTGGGTGA AATCGTCCGC AACATCTTAA TCGACTTCAC CAAGAAGGGA
    TTCCTCTTCC GAGGGCAGAT CTCTGAGACG CTGAAGACCC GGGGCATCTT TGAGACCAAG
    TTTCTCTCTC AGATCGAGAG TGACCGATTA GCACTGCTCC AGGTCCGGGC TATCCTCCAG
    CAGCTAGGTC TGAATAGCAC CTGCGATGAC AGTATCCTCG TCAAGACAGT GTGCGGGGTG
    GTGTCCAGGA GGGCCGCACA GCTGTGTGGC GCAGGCATGG CTGCGGTTGT GGATAAGATC
    CGCGAGAACA GAGGACTGGA CCGTCTGAAT GTGACTGTGG GAGTGGACGG GACACTCTAC
    AAGCTTCATC CACACTTCTC CAGAATCATG CACCAGACGG TGAAGGAACT GTCACCAAAA
    TGTAACGTGT CCTTCCTCCT GTCTGAGGAT GGCAGCGGCA AGGGGGCCGC CCTCATCACG
    GCCGTGGGCG TGCGGTTACG CACAGAGGCA AGCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100382
    Clone ID Related Accession (Same CDS sequence) NM_001322367.1
    Accession Version NM_001322367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Homo sapiens(human)
    Product hexokinase-1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596223.3 and AC016821.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2152568 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
    AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
    ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
    GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
    ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
    AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
    GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
    AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
    TCCAAAATAG ATGAGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001309296.1
    CDS102..2759
    Translation

    Target ORF information:

    RefSeq Version NM_001322367.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hexokinase 1 (HK1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001322367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
    AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
    ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
    GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
    ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
    AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
    GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
    AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
    TCCAAAATAG ATGAGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
    ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
    GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
    GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
    ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
    TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
    ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
    AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
    AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
    CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
    ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
    GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
    CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
    GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
    ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
    GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
    CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
    CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
    GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
    GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
    GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
    GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
    GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
    GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
    GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
    GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
    GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
    TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
    ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
    TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
    GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
    TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
    CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
    CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
    AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
    TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
    ACAGAGGCAA GCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.