UBE2K cDNA ORF clone, Homo sapiens(human)

The following UBE2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu08897 NM_005339.5
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu58211 NM_001312647.2
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $69.30
$99.00
OHu58211 NM_001312648.2
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $69.30
$99.00
OHu08907 NM_001111113.2
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu08919 NM_001111112.2
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu58210 NM_001312646.2
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu58211 NM_001312647.1 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $69.30
$99.00
OHu58210 NM_001312646.1 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu08907 NM_001111113.1 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu08897 NM_005339.4 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu08919 NM_001111112.1 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu58211 NM_001312648.1 Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu08897
    Clone ID Related Accession (Same CDS sequence) NM_005339.5 , NM_005339.4
    Accession Version NM_005339.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, BC022804.1, AC105287.5 and BU622273.1. On Nov 22, 2018 this sequence version replaced NM_005339.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.57829.1, SRR1660803.204950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261427.10/ ENSP00000261427.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGT ACAAACAAAA TCCCGAAATG
    TTCAAACAGA CAGCTCGACT TTGGGCACAT GTGTATGCTG GAGCACCAGT TTCTAGTCCA
    GAATACACCA AAAAAATAGA AAACCTATGT GCTATGGGCT TTGATAGGAA TGCAGTAATA
    GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAATTGCT TCTGAGTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005330.1
    CDS193..795
    Translation

    Target ORF information:

    RefSeq Version NM_005339.5
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005339.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGT ACAAACAAAA TCCCGAAATG
    TTCAAACAGA CAGCTCGACT TTGGGCACAT GTGTATGCTG GAGCACCAGT TTCTAGTCCA
    GAATACACCA AAAAAATAGA AAACCTATGT GCTATGGGCT TTGATAGGAA TGCAGTAATA
    GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAATTGCT TCTGAGTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08897
    Clone ID Related Accession (Same CDS sequence) NM_005339.5 , NM_005339.4
    Accession Version NM_005339.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-09
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, BC022804.1, AC105287.5 and BU622273.1. On Dec 20, 2007 this sequence version replaced NM_005339.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.204950.1, SRR1660807.34585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGT ACAAACAAAA TCCCGAAATG
    TTCAAACAGA CAGCTCGACT TTGGGCACAT GTGTATGCTG GAGCACCAGT TTCTAGTCCA
    GAATACACCA AAAAAATAGA AAACCTATGT GCTATGGGCT TTGATAGGAA TGCAGTAATA
    GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAATTGCT TCTGAGTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005330.1
    CDS285..887
    Translation

    Target ORF information:

    RefSeq Version NM_005339.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005339.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGT ACAAACAAAA TCCCGAAATG
    TTCAAACAGA CAGCTCGACT TTGGGCACAT GTGTATGCTG GAGCACCAGT TTCTAGTCCA
    GAATACACCA AAAAAATAGA AAACCTATGT GCTATGGGCT TTGATAGGAA TGCAGTAATA
    GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAATTGCT TCTGAGTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58211
    Clone ID Related Accession (Same CDS sequence) NM_001312647.1 , NM_001312647.2 , NM_001312648.1 , NM_001312648.2
    Accession Version NM_001312647.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK093533.1, AC105287.5 and BU622273.1. Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon and initiates translation at an alternate downstream start codon compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Variants 5 and 6 both encode the same isoform (5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093533.1, SRR1163655.559194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299576.1
    CDS506..799
    Translation

    Target ORF information:

    RefSeq Version NM_001312647.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58211
    Clone ID Related Accession (Same CDS sequence) NM_001312647.1 , NM_001312647.2 , NM_001312648.1 , NM_001312648.2
    Accession Version NM_001312647.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK093533.1, AC105287.5 and BU622273.1. On May 31, 2019 this sequence version replaced NM_001312647.1. Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon and initiates translation at an alternate downstream start codon compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Variants 5 and 6 both encode the same isoform (5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093533.1, SRR1163655.559194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299576.1
    CDS414..707
    Translation

    Target ORF information:

    RefSeq Version NM_001312647.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312647.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58211
    Clone ID Related Accession (Same CDS sequence) NM_001312647.1 , NM_001312647.2 , NM_001312648.1 , NM_001312648.2
    Accession Version NM_001312648.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK307130.1, AC105287.5 and BU622273.1. Transcript Variant: This variant (6) lacks an alternate exon and initiates translation at an alternate downstream start codon compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Variants 5 and 6 both encode the same isoform (5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.493107.1, AK307130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299577.1
    CDS500..793
    Translation

    Target ORF information:

    RefSeq Version NM_001312648.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58211
    Clone ID Related Accession (Same CDS sequence) NM_001312647.1 , NM_001312647.2 , NM_001312648.1 , NM_001312648.2
    Accession Version NM_001312648.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK307130.1, AC105287.5 and BU622273.1. On May 31, 2019 this sequence version replaced NM_001312648.1. Transcript Variant: This variant (6) lacks an alternate exon and initiates translation at an alternate downstream start codon compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Variants 5 and 6 both encode the same isoform (5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307130.1, SRR1163657.493107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299577.1
    CDS408..701
    Translation

    Target ORF information:

    RefSeq Version NM_001312648.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312648.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGACTCTCC GCACGGTATT ATTGTCATTG CAAGCACTAT TGGCAGCTGC AGAGCCAGAT 
    GATCCACAGG ATGCTGTAGT AGCAAATCAG TACAAACAAA ATCCCGAAAT GTTCAAACAG
    ACAGCTCGAC TTTGGGCACA TGTGTATGCT GGAGCACCAG TTTCTAGTCC AGAATACACC
    AAAAAAATAG AAAACCTATG TGCTATGGGC TTTGATAGGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08907
    Clone ID Related Accession (Same CDS sequence) NM_001111113.2 , NM_001111113.1
    Accession Version NM_001111113.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK291454.1, AC105287.5 and BU622273.1. On May 31, 2019 this sequence version replaced NM_001111113.1. Transcript Variant: This variant (3) lacks an alternate in-frame segment in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.8647.1, SRR1803612.139096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGGTCCGGT TTATCACTAA AATATGGCAT CCTAATATTA GTTCCGTCAC AGGGGCTATT
    TGTTTGGATA TCCTGAAAGA TCAATGGGCA GCTGCAATGA CTCTCCGCAC GGTATTATTG
    TCATTGCAAG CACTATTGGC AGCTGCAGAG CCAGATGATC CACAGGATGC TGTAGTAGCA
    AATCAGTACA AACAAAATCC CGAAATGTTC AAACAGACAG CTCGACTTTG GGCACATGTG
    TATGCTGGAG CACCAGTTTC TAGTCCAGAA TACACCAAAA AAATAGAAAA CCTATGTGCT
    ATGGGCTTTG ATAGGAATGC AGTAATAGTG GCCTTGTCTT CAAAATCATG GGATGTAGAG
    ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104583.1
    CDS193..642
    Translation

    Target ORF information:

    RefSeq Version NM_001111113.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGGTCCGGT TTATCACTAA AATATGGCAT CCTAATATTA GTTCCGTCAC AGGGGCTATT
    TGTTTGGATA TCCTGAAAGA TCAATGGGCA GCTGCAATGA CTCTCCGCAC GGTATTATTG
    TCATTGCAAG CACTATTGGC AGCTGCAGAG CCAGATGATC CACAGGATGC TGTAGTAGCA
    AATCAGTACA AACAAAATCC CGAAATGTTC AAACAGACAG CTCGACTTTG GGCACATGTG
    TATGCTGGAG CACCAGTTTC TAGTCCAGAA TACACCAAAA AAATAGAAAA CCTATGTGCT
    ATGGGCTTTG ATAGGAATGC AGTAATAGTG GCCTTGTCTT CAAAATCATG GGATGTAGAG
    ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08907
    Clone ID Related Accession (Same CDS sequence) NM_001111113.2 , NM_001111113.1
    Accession Version NM_001111113.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-09
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK291454.1, AC105287.5 and BU622273.1. Transcript Variant: This variant (3) lacks an alternate in-frame segment in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.8647.1, SRR1803612.139096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGGTCCGGT TTATCACTAA AATATGGCAT CCTAATATTA GTTCCGTCAC AGGGGCTATT
    TGTTTGGATA TCCTGAAAGA TCAATGGGCA GCTGCAATGA CTCTCCGCAC GGTATTATTG
    TCATTGCAAG CACTATTGGC AGCTGCAGAG CCAGATGATC CACAGGATGC TGTAGTAGCA
    AATCAGTACA AACAAAATCC CGAAATGTTC AAACAGACAG CTCGACTTTG GGCACATGTG
    TATGCTGGAG CACCAGTTTC TAGTCCAGAA TACACCAAAA AAATAGAAAA CCTATGTGCT
    ATGGGCTTTG ATAGGAATGC AGTAATAGTG GCCTTGTCTT CAAAATCATG GGATGTAGAG
    ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104583.1
    CDS285..734
    Translation

    Target ORF information:

    RefSeq Version NM_001111113.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGGTCCGGT TTATCACTAA AATATGGCAT CCTAATATTA GTTCCGTCAC AGGGGCTATT
    TGTTTGGATA TCCTGAAAGA TCAATGGGCA GCTGCAATGA CTCTCCGCAC GGTATTATTG
    TCATTGCAAG CACTATTGGC AGCTGCAGAG CCAGATGATC CACAGGATGC TGTAGTAGCA
    AATCAGTACA AACAAAATCC CGAAATGTTC AAACAGACAG CTCGACTTTG GGCACATGTG
    TATGCTGGAG CACCAGTTTC TAGTCCAGAA TACACCAAAA AAATAGAAAA CCTATGTGCT
    ATGGGCTTTG ATAGGAATGC AGTAATAGTG GCCTTGTCTT CAAAATCATG GGATGTAGAG
    ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08919
    Clone ID Related Accession (Same CDS sequence) NM_001111112.1 , NM_001111112.2
    Accession Version NM_001111112.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-09
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, CX786829.1, AC105287.5 and BU622273.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.224379.1, SRR1660803.206683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104582.1
    CDS285..758
    Translation

    Target ORF information:

    RefSeq Version NM_001111112.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111112.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08919
    Clone ID Related Accession (Same CDS sequence) NM_001111112.1 , NM_001111112.2
    Accession Version NM_001111112.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, CX786829.1, AC105287.5 and BU622273.1. On May 31, 2019 this sequence version replaced NM_001111112.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.224379.1, SRR1660803.206683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104582.1
    CDS193..666
    Translation

    Target ORF information:

    RefSeq Version NM_001111112.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG
    CATCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG
    GCAGCTGCAA TGACTCTCCG CACGGTATTA TTGTCATTGC AAGCACTATT GGCAGCTGCA
    GAGCCAGATG ATCCACAGGA TGCTGTAGTA GCAAATCAGA ATGCAGTAAT AGTGGCCTTG
    TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAATTGC TTCTGAGTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58210
    Clone ID Related Accession (Same CDS sequence) NM_001312646.1 , NM_001312646.2
    Accession Version NM_001312646.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA526283.1, AK295846.1, AC105287.5 and BU622273.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.188789.1, SRR1803612.96842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGTACA AACAAAATCC CGAAATGTTC
    AAACAGACAG CTCGACTTTG GGCACATGTG TATGCTGGAG CACCAGTTTC TAGTCCAGAA
    TACACCAAAA AAATAGAAAA CCTATGTGCT ATGGGCTTTG ATAGGAATGC AGTAATAGTG
    GCCTTGTCTT CAAAATCATG GGATGTAGAG ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299575.1
    CDS285..704
    Translation

    Target ORF information:

    RefSeq Version NM_001312646.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGTACA AACAAAATCC CGAAATGTTC
    AAACAGACAG CTCGACTTTG GGCACATGTG TATGCTGGAG CACCAGTTTC TAGTCCAGAA
    TACACCAAAA AAATAGAAAA CCTATGTGCT ATGGGCTTTG ATAGGAATGC AGTAATAGTG
    GCCTTGTCTT CAAAATCATG GGATGTAGAG ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58210
    Clone ID Related Accession (Same CDS sequence) NM_001312646.1 , NM_001312646.2
    Accession Version NM_001312646.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product ubiquitin-conjugating enzyme E2 K isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295846.1, AC105287.5 and BU622273.1. On May 31, 2019 this sequence version replaced NM_001312646.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.188789.1, SRR1803612.96842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGTACA AACAAAATCC CGAAATGTTC
    AAACAGACAG CTCGACTTTG GGCACATGTG TATGCTGGAG CACCAGTTTC TAGTCCAGAA
    TACACCAAAA AAATAGAAAA CCTATGTGCT ATGGGCTTTG ATAGGAATGC AGTAATAGTG
    GCCTTGTCTT CAAAATCATG GGATGTAGAG ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001299575.1
    CDS193..612
    Translation

    Target ORF information:

    RefSeq Version NM_001312646.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001312646.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
    GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA
    GGAGAAATAG CAGGACCTCC AGACACACCA TATGAAGGAG GAAGATACCA ACTAGAGATA
    AAAATACCAG AAACATACCC ATTTAATCCC CCTAAGTACA AACAAAATCC CGAAATGTTC
    AAACAGACAG CTCGACTTTG GGCACATGTG TATGCTGGAG CACCAGTTTC TAGTCCAGAA
    TACACCAAAA AAATAGAAAA CCTATGTGCT ATGGGCTTTG ATAGGAATGC AGTAATAGTG
    GCCTTGTCTT CAAAATCATG GGATGTAGAG ACTGCAACAG AATTGCTTCT GAGTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
    Nature communications711491(2016 May)
    Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,


    Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
    Molecular systems biology12(4)863(2016 Apr)
    Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP