HCFC1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006724815.3	XP_006724878.1	host cell factor 1 isoform X1
XM_011531148.3	XP_011529450.1	host cell factor 1 isoform X4
XM_011531148.2	XP_011529450.1	host cell factor 1 isoform X4
XM_006724816.3	XP_006724879.1	host cell factor 1 isoform X2
NM_005334.3	NP_005325.2	host cell factor 1
NM_005334.2	NP_005325.2	host cell factor 1
XM_006724815.2	XP_006724878.1	host cell factor 1 isoform X1
XM_017029471.1	XP_016884960.1	host cell factor 1 isoform X5
XM_011531147.3	XP_011529449.1	host cell factor 1 isoform X3
XM_017029471.2	XP_016884960.1	host cell factor 1 isoform X5
XM_011531147.2	XP_011529449.1	host cell factor 1 isoform X3
XM_006724816.2	XP_006724879.1	host cell factor 1 isoform X2
XM_017029472.1	XP_016884961.1	host cell factor 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28100	NM_005334.3 Latest version!	Homo sapiens host cell factor C1 (HCFC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu54394	XM_006724815.3 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu54395	XM_006724816.3 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu58182	XM_011531147.3 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu58183	XM_011531148.3 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu99282	XM_017029471.2 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu99283	XM_017029472.1 Latest version!	Homo sapiens host cell factor C1 (HCFC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu58183	XM_011531148.2	Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu28100	NM_005334.2	Homo sapiens host cell factor C1 (HCFC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu54394	XM_006724815.2	Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu99282	XM_017029471.1	Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu58182	XM_011531147.2	Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu54395	XM_006724816.2	Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

HCFC1 ( NM_005334.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005325.2 Homo sapiens host cell factor C1 (HCFC1), mRNA.

Price & Availability↑

CloneID	OHu28100
Clone ID Related Accession (Same CDS sequence)	NM_005334.3 , NM_005334.2
Accession Version	NM_005334.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	host cell factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U52112.2, BC063435.1, L20010.1 and BC010606.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005334.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L20010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000310441.12/ ENSP00000309555.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGA AGCCAGACCT GCCGCCCCCA 
CCCAGCAAAG CCCCCATGAA GAAGGAAAAC CAGTGGTTTG ATGTGGGAGT CATTAAGGGC 
ACCAATGTAA TGGTGACACA CTATTTCCTG CCACCAGATG ATGCTGTCCC ATCAGACGAT 
GATTTGGGCA CCGTCCCTGA CTATAACCAG CTGAAGAAGC AGGAGCTGCA GCCAGGCACA 
GCCTATAAGT TTCGTGTTGC CGGAATCAAT GCCTGTGGCC GGGGGCCCTT CAGCGAAATC 
TCAGCCTTTA AGACGTGCCT GCCTGGTTTC CCAGGGGCCC CTTGTGCCAT TAAAATCAGC 
AAAAGTCCGG ATGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATT 
ATCGAGTACT CCGTGTACCT GGCCATCCAG AGCTCACAGG CTGGGGGCGA GCTCAAGAGC 
TCCACCCCGG CCCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTG 
GTGCAGTCCT CCAGCCTTTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC 
TTCCGCATCG CCGCCCGCAA TGAGAAGGGC TATGGCCCGG CCACACAAGT GAGGTGGCTG 
CAGGAAACCA GTAAAGACAG CTCTGGCACC AAGCCAGCCA ACAAGCGGCC CATGTCCTCT 
CCAGAAATGA AATCTGCTCC AAAGAAATCT AAGGCCGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005325.2
CDS	979..7086
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	NM_005334.3
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005334.3

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGA AGCCAGACCT GCCGCCCCCA 
CCCAGCAAAG CCCCCATGAA GAAGGAAAAC CAGTGGTTTG ATGTGGGAGT CATTAAGGGC 
ACCAATGTAA TGGTGACACA CTATTTCCTG CCACCAGATG ATGCTGTCCC ATCAGACGAT 
GATTTGGGCA CCGTCCCTGA CTATAACCAG CTGAAGAAGC AGGAGCTGCA GCCAGGCACA 
GCCTATAAGT TTCGTGTTGC CGGAATCAAT GCCTGTGGCC GGGGGCCCTT CAGCGAAATC 
TCAGCCTTTA AGACGTGCCT GCCTGGTTTC CCAGGGGCCC CTTGTGCCAT TAAAATCAGC 
AAAAGTCCGG ATGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATT 
ATCGAGTACT CCGTGTACCT GGCCATCCAG AGCTCACAGG CTGGGGGCGA GCTCAAGAGC 
TCCACCCCGG CCCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTG 
GTGCAGTCCT CCAGCCTTTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC 
TTCCGCATCG CCGCCCGCAA TGAGAAGGGC TATGGCCCGG CCACACAAGT GAGGTGGCTG 
CAGGAAACCA GTAAAGACAG CTCTGGCACC AAGCCAGCCA ACAAGCGGCC CATGTCCTCT 
CCAGAAATGA AATCTGCTCC AAAGAAATCT AAGGCCGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( NM_005334.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005325.2 Homo sapiens host cell factor C1 (HCFC1), mRNA.

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CloneID	OHu28100
Clone ID Related Accession (Same CDS sequence)	NM_005334.3 , NM_005334.2
Accession Version	NM_005334.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	host cell factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC063435.1, L20010.1 and BC010606.1. This sequence is a reference standard in the RefSeqGene project. On May 19, 2006 this sequence version replaced NM_005334.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L20010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGA AGCCAGACCT GCCGCCCCCA 
CCCAGCAAAG CCCCCATGAA GAAGGAAAAC CAGTGGTTTG ATGTGGGAGT CATTAAGGGC 
ACCAATGTAA TGGTGACACA CTATTTCCTG CCACCAGATG ATGCTGTCCC ATCAGACGAT 
GATTTGGGCA CCGTCCCTGA CTATAACCAG CTGAAGAAGC AGGAGCTGCA GCCAGGCACA 
GCCTATAAGT TTCGTGTTGC CGGAATCAAT GCCTGTGGCC GGGGGCCCTT CAGCGAAATC 
TCAGCCTTTA AGACGTGCCT GCCTGGTTTC CCAGGGGCCC CTTGTGCCAT TAAAATCAGC 
AAAAGTCCGG ATGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATT 
ATCGAGTACT CCGTGTACCT GGCCATCCAG AGCTCACAGG CTGGGGGCGA GCTCAAGAGC 
TCCACCCCGG CCCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTG 
GTGCAGTCCT CCAGCCTTTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC 
TTCCGCATCG CCGCCCGCAA TGAGAAGGGC TATGGCCCGG CCACACAAGT GAGGTGGCTG 
CAGGAAACCA GTAAAGACAG CTCTGGCACC AAGCCAGCCA ACAAGCGGCC CATGTCCTCT 
CCAGAAATGA AATCTGCTCC AAAGAAATCT AAGGCCGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005325.2
CDS	529..6636
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	NM_005334.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005334.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGA AGCCAGACCT GCCGCCCCCA 
CCCAGCAAAG CCCCCATGAA GAAGGAAAAC CAGTGGTTTG ATGTGGGAGT CATTAAGGGC 
ACCAATGTAA TGGTGACACA CTATTTCCTG CCACCAGATG ATGCTGTCCC ATCAGACGAT 
GATTTGGGCA CCGTCCCTGA CTATAACCAG CTGAAGAAGC AGGAGCTGCA GCCAGGCACA 
GCCTATAAGT TTCGTGTTGC CGGAATCAAT GCCTGTGGCC GGGGGCCCTT CAGCGAAATC 
TCAGCCTTTA AGACGTGCCT GCCTGGTTTC CCAGGGGCCC CTTGTGCCAT TAAAATCAGC 
AAAAGTCCGG ATGGTGCTCA CCTCACCTGG GAGCCACCCT CTGTGACCTC CGGCAAGATT 
ATCGAGTACT CCGTGTACCT GGCCATCCAG AGCTCACAGG CTGGGGGCGA GCTCAAGAGC 
TCCACCCCGG CCCAGCTGGC CTTCATGCGG GTGTACTGCG GGCCCAGCCC CTCCTGCCTG 
GTGCAGTCCT CCAGCCTTTC CAACGCCCAC ATCGACTACA CCACCAAGCC CGCCATCATC 
TTCCGCATCG CCGCCCGCAA TGAGAAGGGC TATGGCCCGG CCACACAAGT GAGGTGGCTG 
CAGGAAACCA GTAAAGACAG CTCTGGCACC AAGCCAGCCA ACAAGCGGCC CATGTCCTCT 
CCAGAAATGA AATCTGCTCC AAAGAAATCT AAGGCCGATG GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_006724815.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724878.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.

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CloneID	OHu54394
Clone ID Related Accession (Same CDS sequence)	XM_006724815.3 , XM_006724815.2
Accession Version	XM_006724815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006724815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGCAGGCA CCGGCGAGCC CATGGACACC 
TCCGAGGCAG CAGCAACCGT GACTCAGGCG GAGCTGGGGC ACCTGTCGGC CGAGGGTCAG 
GAGGGCCAGG CCACCACCAT ACCCATTGTG CTGACACAGC AGGAGCTGGC TGCCCTGGTG 
CAGCAGCAGC AGCTGCAGGA GGCCCAGGCC CAGCAGCAGC ATCACCACCT CCCCACTGAG 
GCCCTGGCCC CTGCCGACAG TCTCAACGAC CCAGCCATTG AGAGCAATTG CCTCAATGAG 
CTGGCCGGCA CGGTCCCCAG CACTGTGGCG CTGCTGCCCT CAACGGCCAC TGAGAGCCTG 
GCTCCATCCA ACACATTTGT GGCCCCCCAG CCGGTTGTGG TGGCCAGCCC AGCCAAGCTG 
CAGGCTGCAG CTACCCTGAC CGAAGTGGCC AATGGCATCG AGTCCCTGGG TGTGAAGCCA 
GACCTGCCGC CCCCACCCAG CAAAGCCCCC ATGAAGAAGG AAAACCAGTG GTTTGATGTG 
GGAGTCATTA AGGGCACCAA TGTAATGGTG ACACACTATT TCCTGCCACC AGATGATGCT 
GTCCCATCAG ACGATGATTT GGGCACCGTC CCTGACTATA ACCAGCTGAA GAAGCAGGAG 
CTGCAGCCAG GCACAGCCTA TAAGTTTCGT GTTGCCGGAA TCAATGCCTG TGGCCGGGGG 
CCCTTCAGCG AAATCTCAGC CTTTAAGACG TGCCTGCCTG GTTTCCCAGG GGCCCCTTGT 
GCCATTAAAA TCAGCAAAAG TCCGGATGGT GCTCACCTCA CCTGGGAGCC ACCCTCTGTG 
ACCTCCGGCA AGATTATCGA GTACTCCGTG TACCTGGCCA TCCAGAGCTC ACAGGCTGGG 
GGCGAGCTCA AGAGCTCCAC CCCGGCCCAG CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC 
AGCCCCTCCT GCCTGGTGCA GTCCTCCAGC CTTTCCAACG CCCACATCGA CTACACCACC 
AAGCCCGCCA TCATCTTCCG CATCGCCGCC CGCAATGAGA AGGGCTATGG CCCGGCCACA 
CAAGTGAGGT GGCTGCAGGA AACCAGTAAA GACAGCTCTG GCACCAAGCC AGCCAACAAG 
CGGCCCATGT CCTCTCCAGA AATGAAATCT GCTCCAAAGA AATCTAAGGC CGATGGTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724878.1
CDS	1520..7762
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006724815.3
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724815.3

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGCAGGCA CCGGCGAGCC CATGGACACC 
TCCGAGGCAG CAGCAACCGT GACTCAGGCG GAGCTGGGGC ACCTGTCGGC CGAGGGTCAG 
GAGGGCCAGG CCACCACCAT ACCCATTGTG CTGACACAGC AGGAGCTGGC TGCCCTGGTG 
CAGCAGCAGC AGCTGCAGGA GGCCCAGGCC CAGCAGCAGC ATCACCACCT CCCCACTGAG 
GCCCTGGCCC CTGCCGACAG TCTCAACGAC CCAGCCATTG AGAGCAATTG CCTCAATGAG 
CTGGCCGGCA CGGTCCCCAG CACTGTGGCG CTGCTGCCCT CAACGGCCAC TGAGAGCCTG 
GCTCCATCCA ACACATTTGT GGCCCCCCAG CCGGTTGTGG TGGCCAGCCC AGCCAAGCTG 
CAGGCTGCAG CTACCCTGAC CGAAGTGGCC AATGGCATCG AGTCCCTGGG TGTGAAGCCA 
GACCTGCCGC CCCCACCCAG CAAAGCCCCC ATGAAGAAGG AAAACCAGTG GTTTGATGTG 
GGAGTCATTA AGGGCACCAA TGTAATGGTG ACACACTATT TCCTGCCACC AGATGATGCT 
GTCCCATCAG ACGATGATTT GGGCACCGTC CCTGACTATA ACCAGCTGAA GAAGCAGGAG 
CTGCAGCCAG GCACAGCCTA TAAGTTTCGT GTTGCCGGAA TCAATGCCTG TGGCCGGGGG 
CCCTTCAGCG AAATCTCAGC CTTTAAGACG TGCCTGCCTG GTTTCCCAGG GGCCCCTTGT 
GCCATTAAAA TCAGCAAAAG TCCGGATGGT GCTCACCTCA CCTGGGAGCC ACCCTCTGTG 
ACCTCCGGCA AGATTATCGA GTACTCCGTG TACCTGGCCA TCCAGAGCTC ACAGGCTGGG 
GGCGAGCTCA AGAGCTCCAC CCCGGCCCAG CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC 
AGCCCCTCCT GCCTGGTGCA GTCCTCCAGC CTTTCCAACG CCCACATCGA CTACACCACC 
AAGCCCGCCA TCATCTTCCG CATCGCCGCC CGCAATGAGA AGGGCTATGG CCCGGCCACA 
CAAGTGAGGT GGCTGCAGGA AACCAGTAAA GACAGCTCTG GCACCAAGCC AGCCAACAAG 
CGGCCCATGT CCTCTCCAGA AATGAAATCT GCTCCAAAGA AATCTAAGGC CGATGGTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_006724815.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724878.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.

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CloneID	OHu54394
Clone ID Related Accession (Same CDS sequence)	XM_006724815.3 , XM_006724815.2
Accession Version	XM_006724815.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGCAGGCA CCGGCGAGCC CATGGACACC 
TCCGAGGCAG CAGCAACCGT GACTCAGGCG GAGCTGGGGC ACCTGTCGGC CGAGGGTCAG 
GAGGGCCAGG CCACCACCAT ACCCATTGTG CTGACACAGC AGGAGCTGGC TGCCCTGGTG 
CAGCAGCAGC AGCTGCAGGA GGCCCAGGCC CAGCAGCAGC ATCACCACCT CCCCACTGAG 
GCCCTGGCCC CTGCCGACAG TCTCAACGAC CCAGCCATTG AGAGCAATTG CCTCAATGAG 
CTGGCCGGCA CGGTCCCCAG CACTGTGGCG CTGCTGCCCT CAACGGCCAC TGAGAGCCTG 
GCTCCATCCA ACACATTTGT GGCCCCCCAG CCGGTTGTGG TGGCCAGCCC AGCCAAGCTG 
CAGGCTGCAG CTACCCTGAC CGAAGTGGCC AATGGCATCG AGTCCCTGGG TGTGAAGCCA 
GACCTGCCGC CCCCACCCAG CAAAGCCCCC ATGAAGAAGG AAAACCAGTG GTTTGATGTG 
GGAGTCATTA AGGGCACCAA TGTAATGGTG ACACACTATT TCCTGCCACC AGATGATGCT 
GTCCCATCAG ACGATGATTT GGGCACCGTC CCTGACTATA ACCAGCTGAA GAAGCAGGAG 
CTGCAGCCAG GCACAGCCTA TAAGTTTCGT GTTGCCGGAA TCAATGCCTG TGGCCGGGGG 
CCCTTCAGCG AAATCTCAGC CTTTAAGACG TGCCTGCCTG GTTTCCCAGG GGCCCCTTGT 
GCCATTAAAA TCAGCAAAAG TCCGGATGGT GCTCACCTCA CCTGGGAGCC ACCCTCTGTG 
ACCTCCGGCA AGATTATCGA GTACTCCGTG TACCTGGCCA TCCAGAGCTC ACAGGCTGGG 
GGCGAGCTCA AGAGCTCCAC CCCGGCCCAG CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC 
AGCCCCTCCT GCCTGGTGCA GTCCTCCAGC CTTTCCAACG CCCACATCGA CTACACCACC 
AAGCCCGCCA TCATCTTCCG CATCGCCGCC CGCAATGAGA AGGGCTATGG CCCGGCCACA 
CAAGTGAGGT GGCTGCAGGA AACCAGTAAA GACAGCTCTG GCACCAAGCC AGCCAACAAG 
CGGCCCATGT CCTCTCCAGA AATGAAATCT GCTCCAAAGA AATCTAAGGC CGATGGTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724878.1
CDS	994..7236
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006724815.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724815.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGCAGGCA CCGGCGAGCC CATGGACACC 
TCCGAGGCAG CAGCAACCGT GACTCAGGCG GAGCTGGGGC ACCTGTCGGC CGAGGGTCAG 
GAGGGCCAGG CCACCACCAT ACCCATTGTG CTGACACAGC AGGAGCTGGC TGCCCTGGTG 
CAGCAGCAGC AGCTGCAGGA GGCCCAGGCC CAGCAGCAGC ATCACCACCT CCCCACTGAG 
GCCCTGGCCC CTGCCGACAG TCTCAACGAC CCAGCCATTG AGAGCAATTG CCTCAATGAG 
CTGGCCGGCA CGGTCCCCAG CACTGTGGCG CTGCTGCCCT CAACGGCCAC TGAGAGCCTG 
GCTCCATCCA ACACATTTGT GGCCCCCCAG CCGGTTGTGG TGGCCAGCCC AGCCAAGCTG 
CAGGCTGCAG CTACCCTGAC CGAAGTGGCC AATGGCATCG AGTCCCTGGG TGTGAAGCCA 
GACCTGCCGC CCCCACCCAG CAAAGCCCCC ATGAAGAAGG AAAACCAGTG GTTTGATGTG 
GGAGTCATTA AGGGCACCAA TGTAATGGTG ACACACTATT TCCTGCCACC AGATGATGCT 
GTCCCATCAG ACGATGATTT GGGCACCGTC CCTGACTATA ACCAGCTGAA GAAGCAGGAG 
CTGCAGCCAG GCACAGCCTA TAAGTTTCGT GTTGCCGGAA TCAATGCCTG TGGCCGGGGG 
CCCTTCAGCG AAATCTCAGC CTTTAAGACG TGCCTGCCTG GTTTCCCAGG GGCCCCTTGT 
GCCATTAAAA TCAGCAAAAG TCCGGATGGT GCTCACCTCA CCTGGGAGCC ACCCTCTGTG 
ACCTCCGGCA AGATTATCGA GTACTCCGTG TACCTGGCCA TCCAGAGCTC ACAGGCTGGG 
GGCGAGCTCA AGAGCTCCAC CCCGGCCCAG CTGGCCTTCA TGCGGGTGTA CTGCGGGCCC 
AGCCCCTCCT GCCTGGTGCA GTCCTCCAGC CTTTCCAACG CCCACATCGA CTACACCACC 
AAGCCCGCCA TCATCTTCCG CATCGCCGCC CGCAATGAGA AGGGCTATGG CCCGGCCACA 
CAAGTGAGGT GGCTGCAGGA AACCAGTAAA GACAGCTCTG GCACCAAGCC AGCCAACAAG 
CGGCCCATGT CCTCTCCAGA AATGAAATCT GCTCCAAAGA AATCTAAGGC CGATGGTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_006724816.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724879.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.

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CloneID	OHu54395
Clone ID Related Accession (Same CDS sequence)	XM_006724816.3 , XM_006724816.2
Accession Version	XM_006724816.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006724816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC 
GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG 
GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC 
CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG 
GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT 
CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG 
GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724879.1
CDS	1519..7758
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006724816.3
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724816.3

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC 
GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG 
GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC 
CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG 
GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT 
CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG 
GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_006724816.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724879.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.

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CloneID	OHu54395
Clone ID Related Accession (Same CDS sequence)	XM_006724816.3 , XM_006724816.2
Accession Version	XM_006724816.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC 
GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG 
GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC 
CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG 
GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT 
CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG 
GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724879.1
CDS	994..7233
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_006724816.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724816.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGAAG 
ATCTCATCAA TGACTGAGAC TGCCCCAAGG GCTCTGACTA CTGAAGTCCC CATCCCGGCC 
AAAATAACAG TGACCATAGC CAACACAGAA ACTTCTGACA TGCCCTTCTC TGCTGTTGAC 
ATCCTGCAGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA 
CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA 
GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT 
TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA 
CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA 
GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT 
GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG 
GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC 
GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG 
GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC 
CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG 
GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT 
CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG 
GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_011531147.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529449.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.

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CloneID	OHu58182
Clone ID Related Accession (Same CDS sequence)	XM_011531147.3 , XM_011531147.2
Accession Version	XM_011531147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGCAGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGGTGAGTCG GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529449.1
CDS	1520..7639
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVVSRKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_011531147.3
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531147.3

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGCAGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGGTGAGTCG GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_011531147.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529449.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.

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CloneID	OHu58182
Clone ID Related Accession (Same CDS sequence)	XM_011531147.3 , XM_011531147.2
Accession Version	XM_011531147.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGCAGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGGTGAGTCG GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529449.1
CDS	994..7113
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVVSRKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_011531147.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531147.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGCAGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGGTGAGTCG GAAGCCAGAC 
CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA 
GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC 
CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG 
CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC 
TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC 
ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC 
TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC 
GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC 
CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG 
CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA 
GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG 
CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_011531148.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529450.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.

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CloneID	OHu58183
Clone ID Related Accession (Same CDS sequence)	XM_011531148.3 , XM_011531148.2
Accession Version	XM_011531148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGG TGAGTCGGAA GCCAGACCTG 
CCGCCCCCAC CCAGCAAAGC CCCCATGAAG AAGGAAAACC AGTGGTTTGA TGTGGGAGTC 
ATTAAGGGCA CCAATGTAAT GGTGACACAC TATTTCCTGC CACCAGATGA TGCTGTCCCA 
TCAGACGATG ATTTGGGCAC CGTCCCTGAC TATAACCAGC TGAAGAAGCA GGAGCTGCAG 
CCAGGCACAG CCTATAAGTT TCGTGTTGCC GGAATCAATG CCTGTGGCCG GGGGCCCTTC 
AGCGAAATCT CAGCCTTTAA GACGTGCCTG CCTGGTTTCC CAGGGGCCCC TTGTGCCATT 
AAAATCAGCA AAAGTCCGGA TGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC 
GGCAAGATTA TCGAGTACTC CGTGTACCTG GCCATCCAGA GCTCACAGGC TGGGGGCGAG 
CTCAAGAGCT CCACCCCGGC CCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC 
TCCTGCCTGG TGCAGTCCTC CAGCCTTTCC AACGCCCACA TCGACTACAC CACCAAGCCC 
GCCATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGCT ATGGCCCGGC CACACAAGTG 
AGGTGGCTGC AGGAAACCAG TAAAGACAGC TCTGGCACCA AGCCAGCCAA CAAGCGGCCC 
ATGTCCTCTC CAGAAATGAA ATCTGCTCCA AAGAAATCTA AGGCCGATGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529450.1
CDS	1519..7635
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVVSRKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_011531148.3
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531148.3

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGG TGAGTCGGAA GCCAGACCTG 
CCGCCCCCAC CCAGCAAAGC CCCCATGAAG AAGGAAAACC AGTGGTTTGA TGTGGGAGTC 
ATTAAGGGCA CCAATGTAAT GGTGACACAC TATTTCCTGC CACCAGATGA TGCTGTCCCA 
TCAGACGATG ATTTGGGCAC CGTCCCTGAC TATAACCAGC TGAAGAAGCA GGAGCTGCAG 
CCAGGCACAG CCTATAAGTT TCGTGTTGCC GGAATCAATG CCTGTGGCCG GGGGCCCTTC 
AGCGAAATCT CAGCCTTTAA GACGTGCCTG CCTGGTTTCC CAGGGGCCCC TTGTGCCATT 
AAAATCAGCA AAAGTCCGGA TGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC 
GGCAAGATTA TCGAGTACTC CGTGTACCTG GCCATCCAGA GCTCACAGGC TGGGGGCGAG 
CTCAAGAGCT CCACCCCGGC CCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC 
TCCTGCCTGG TGCAGTCCTC CAGCCTTTCC AACGCCCACA TCGACTACAC CACCAAGCCC 
GCCATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGCT ATGGCCCGGC CACACAAGTG 
AGGTGGCTGC AGGAAACCAG TAAAGACAGC TCTGGCACCA AGCCAGCCAA CAAGCGGCCC 
ATGTCCTCTC CAGAAATGAA ATCTGCTCCA AAGAAATCTA AGGCCGATGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_011531148.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529450.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.

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CloneID	OHu58183
Clone ID Related Accession (Same CDS sequence)	XM_011531148.3 , XM_011531148.2
Accession Version	XM_011531148.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGG TGAGTCGGAA GCCAGACCTG 
CCGCCCCCAC CCAGCAAAGC CCCCATGAAG AAGGAAAACC AGTGGTTTGA TGTGGGAGTC 
ATTAAGGGCA CCAATGTAAT GGTGACACAC TATTTCCTGC CACCAGATGA TGCTGTCCCA 
TCAGACGATG ATTTGGGCAC CGTCCCTGAC TATAACCAGC TGAAGAAGCA GGAGCTGCAG 
CCAGGCACAG CCTATAAGTT TCGTGTTGCC GGAATCAATG CCTGTGGCCG GGGGCCCTTC 
AGCGAAATCT CAGCCTTTAA GACGTGCCTG CCTGGTTTCC CAGGGGCCCC TTGTGCCATT 
AAAATCAGCA AAAGTCCGGA TGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC 
GGCAAGATTA TCGAGTACTC CGTGTACCTG GCCATCCAGA GCTCACAGGC TGGGGGCGAG 
CTCAAGAGCT CCACCCCGGC CCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC 
TCCTGCCTGG TGCAGTCCTC CAGCCTTTCC AACGCCCACA TCGACTACAC CACCAAGCCC 
GCCATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGCT ATGGCCCGGC CACACAAGTG 
AGGTGGCTGC AGGAAACCAG TAAAGACAGC TCTGGCACCA AGCCAGCCAA CAAGCGGCCC 
ATGTCCTCTC CAGAAATGAA ATCTGCTCCA AAGAAATCTA AGGCCGATGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529450.1
CDS	994..7110
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVVSRKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_011531148.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531148.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAT TGGCAGTAGC 
CCACAGATGA GTGGGATGGC CGCACTGGCC GCTGCGGCCG CTGCCACCCA GAAGATCCCC 
CCTTCCTCGG CACCCACGGT GCTGAGTGTC CCAGCGGGTA CCACCATCGT GAAGACCATG 
GCTGTGACAC CTGGCACTAC CACCCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC 
ATGGTGAGCA ACCCTGCCAC TCGCATGCTG AAGACTGCAG CCGCCCAGGT GGGGACATCG 
GTTTCCTCCG CCACCAACAC GTCTACCCGC CCTATCATCA CAGTGCACAA GTCAGGCACT 
GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTTG TGGGCGGGGT CACCAAGACC 
ATCACCCTGG TGAAGAGCCC CATCTCTGTC CCAGGAGGCA GTGCTCTGAT TTCCAATCTG 
GGCAAAGTGA TGTCGGTGGT CCAGACCAAA CCAGTTCAGA CTTCAGCAGT CACAGGCCAG 
GCGTCCACGG GTCCTGTGAC TCAGATCATC CAGACCAAAG GGCCCCTGCC AGCGGGAACA 
ATCCTGAAGC TGGTGACCTC AGCAGATGGC AAGCCCACCA CCATCATCAC TACCACGCAG 
GCCAGTGGGG CGGGGACCAA GCCCACCATC CTGGGCATCA GCAGCGTCTC CCCCAGTACC 
ACCAAGCCCG GCACGACCAC CATCATCAAA ACCATCCCCA TGTCGGCCAT CATCACCCAG 
GCGGGCGCCA CGGGTGTGAC CAGCAGTCCT GGCATCAAGT CCCCCATCAC CATCATCACC 
ACCAAGGTGA TGACTTCAGG AACTGGAGCA CCTGCGAAAA TCATCACTGC TGTCCCCAAA 
ATTGCCACTG GCCACGGGCA GCAGGGAGTG ACCCAGGTGG TGCTTAAGGG GGCCCCGGGA 
CAGCCAGGCA CCATCCTCCG CACTGTGCCC ATGGGGGGTG TTCGCCTGGT CACACCCGTC 
ACCGTCTCCG CCGTCAAGCC AGCCGTCACC ACGTTGGTTG TGAAAGGCAC CACAGGTGTC 
ACGACCCTAG GCACAGTGAC AGGCACCGTC TCCACCAGCC TTGCCGGGGC GGGGGGCCAC 
AGCACTAGTG CTTCCCTGGC CACGCCCATC ACCACCTTGG GCACCATTGC CACCCTCTCA 
AGCCAGGTGA TCAACCCCAC TGCCATCACT GTGTCGGCCG CACAGACCAC GCTGACAGCG 
GCAGGCGGGC TCACAACCCC AACCATCACC ATGCAGCCCG TGTCCCAGCC CACCCAGGTA 
ACTCTGATCA CGGCACCTAG TGGGGTGGAG GCCCAGCCTG TGCATGACCT CCCTGTGTCC 
ATTCTGGCCT CCCCGACTAC AGAACAGCCC ACCGCCACAG TTACCATCGC CGACTCAGGC 
CAGGGTGATG TGCAGCCTGG CACTGTCACC TTGGTGTGCT CCAACCCACC CTGTGAGACC 
CACGAGACTG GCACCACCAA CACGGCCACC ACTACTGTTG TGGCTAACCT TGGGGGACAC 
CCCCAGCCCA CCCAAGTGCA GTTCGTCTGT GACAGACAGG AGGCAGCTGC TTCTCTTGTG 
ACCTCGACTG TGGGCCAGCA GAATGGTAGC GTGGTCCGAG TCTGTTCGAA CCCGCCCTGC 
GAGACCCACG AGACGGGCAC CACCAACACC GCCACCACCG CCACCTCCAA CATGGCCGGG 
CAGCATGGCT GCTCAAACCC ACCCTGCGAG ACCCACGAGA CGGGCACCAC CAACACTGCC 
ACTACAGCCA TGTCGAGCGT CGGCGCCAAC CACCAGCGAG ATGCCCGTCG GGCCTGTGCA 
GCTGGCACCC CTGCCGTGAT CCGGATCAGT GTGGCCACTG GGGCGCTGGA GGCAGCCCAG 
GGCTCTAAGT CCCAGTGCCA AACCCGCCAG ACCAGCGCGA CCAGCACCAC CATGACTGTG 
ATGGCCACCG GGGCCCCGTG CTCGGCCGGC CCACTCCTTG GGCCGAGCAT GGCACGGGAG 
CCCGGGGGCC GCAGCCCTGC TTTTGTGCAG TTGGCCCCTC TGAGCAGCAA AGTCAGGCTG 
AGCAGCCCAA GCATTAAGGA CCTTCCTGCG GGGCGCCACA GCCATGCGGT CAGCACCGCT 
GCCATGACCC GTTCCAGCGT GGGTGCTGGG GAGCCCCGCA TGGCACCTGT GTGCGAGAGC 
CTCCAGGGTG GCTCGCCCAG CACCACAGTG ACTGTGACAG CCCTGGAGGC ACTGCTGTGC 
CCCTCGGCCA CCGTGACCCA AGTCTGCTCC AACCCACCAT GTGAGACCCA CGAGACAGGC 
ACCACCAACA CCGCCACTAC CTCGAATGCA GGCAGCGCCC AGAGGGTGTG CTCCAACCCG 
CCATGCGAGA CCCACGAGAC GGGCACCACC CACACGGCCA CCACCGCTAC TTCAAACGGG 
GGCACGGGCC AGCCCGAGGG TGGGCAGCAG CCCCCTGCTG GTCGCCCCTG TGAGACACAC 
CAGACCACTT CCACTGGCAC CACCATGTCG GTCAGCGTGG GTGCCCTGCT TCCCGACGCC 
ACTTCTTCCC ACAGGACCGT GGAGTCTGGC CTAGAGGTGG CGGCGGCACC CAGCGTCACC 
CCCCAGGCTG GCACCGCGCT GCTGGCTCCT TTCCCAACAC AGAGGGTGTG CTCCAACCCC 
CCCTGTGAGA CCCACGAGAC GGGCACCACT CACACGGCCA CCACTGTCAC TTCCAACATG 
AGTTCAAACC AAGACCCCCC ACCTGCTGCC AGCGATCAGG GAGAGGTGGA GAGCACCCAG 
GGCGACAGCG TGAACATCAC CAGCTCCAGT GCCATCACGA CAACCGTGTC CTCCACACTG 
ACGCGGGCTG TGACCACCGT GACGCAGTCC ACACCGGTCC CGGGCCCCTC TGTGCCGCCC 
CCAGAGGAAC TCCAGGTGTC GCCAGGTCCT CGCCAGCAGC TGCCGCCACG GCAGCTTCTG 
CAGTCGGCTT CCACAGCCCT GATGGGGGAG TCCGCCGAGG TCCTGTCAGC CTCCCAGACC 
CCTGAGCTCC CGGCCGCCGT GGATCTGAGC AGCACAGGGG AGCCATCTTC GGGCCAGGAG 
TCTGCCGGCT CTGCGGTGGT GGCCACTGTG GTGGTCCAGC CACCCCCACC CACACAGTCC 
GAAGTAGACC AGTTATCACT TCCCCAAGAG CTAATGGCCG AGGCCCAAGC TGGCACCACC 
ACCCTCATGG TAACGGGGCT CACCCCCGAG GAGCTGGCAG TGACGGCTGC TGCAGAAGCA 
GCTGCCCAGG CCGCAGCCAC GGAGGAAGCC CAGGCCCTGG CCATCCAGGC GGTGCTCCAG 
GCCGCGCAGC AGGCCGTCAT GGGCACCGGC GAGCCCATGG ACACCTCCGA GGCAGCAGCA 
ACCGTGACTC AGGCGGAGCT GGGGCACCTG TCGGCCGAGG GTCAGGAGGG CCAGGCCACC 
ACCATACCCA TTGTGCTGAC ACAGCAGGAG CTGGCTGCCC TGGTGCAGCA GCAGCAGCTG 
CAGGAGGCCC AGGCCCAGCA GCAGCATCAC CACCTCCCCA CTGAGGCCCT GGCCCCTGCC 
GACAGTCTCA ACGACCCAGC CATTGAGAGC AATTGCCTCA ATGAGCTGGC CGGCACGGTC 
CCCAGCACTG TGGCGCTGCT GCCCTCAACG GCCACTGAGA GCCTGGCTCC ATCCAACACA 
TTTGTGGCCC CCCAGCCGGT TGTGGTGGCC AGCCCAGCCA AGCTGCAGGC TGCAGCTACC 
CTGACCGAAG TGGCCAATGG CATCGAGTCC CTGGGTGTGG TGAGTCGGAA GCCAGACCTG 
CCGCCCCCAC CCAGCAAAGC CCCCATGAAG AAGGAAAACC AGTGGTTTGA TGTGGGAGTC 
ATTAAGGGCA CCAATGTAAT GGTGACACAC TATTTCCTGC CACCAGATGA TGCTGTCCCA 
TCAGACGATG ATTTGGGCAC CGTCCCTGAC TATAACCAGC TGAAGAAGCA GGAGCTGCAG 
CCAGGCACAG CCTATAAGTT TCGTGTTGCC GGAATCAATG CCTGTGGCCG GGGGCCCTTC 
AGCGAAATCT CAGCCTTTAA GACGTGCCTG CCTGGTTTCC CAGGGGCCCC TTGTGCCATT 
AAAATCAGCA AAAGTCCGGA TGGTGCTCAC CTCACCTGGG AGCCACCCTC TGTGACCTCC 
GGCAAGATTA TCGAGTACTC CGTGTACCTG GCCATCCAGA GCTCACAGGC TGGGGGCGAG 
CTCAAGAGCT CCACCCCGGC CCAGCTGGCC TTCATGCGGG TGTACTGCGG GCCCAGCCCC 
TCCTGCCTGG TGCAGTCCTC CAGCCTTTCC AACGCCCACA TCGACTACAC CACCAAGCCC 
GCCATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGCT ATGGCCCGGC CACACAAGTG 
AGGTGGCTGC AGGAAACCAG TAAAGACAGC TCTGGCACCA AGCCAGCCAA CAAGCGGCCC 
ATGTCCTCTC CAGAAATGAA ATCTGCTCCA AAGAAATCTA AGGCCGATGG TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_017029471.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884960.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.

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CloneID	OHu99282
Clone ID Related Accession (Same CDS sequence)	XM_017029471.1 , XM_017029471.2
Accession Version	XM_017029471.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAG CAACCCTGCC 
ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC 
ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA 
GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC 
CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG 
GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG 
ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC 
TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC 
AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC 
ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG 
ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA 
GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG 
CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC 
CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG 
CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG 
ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG 
GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC 
ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC 
CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT 
AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT 
ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT 
GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC 
AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG 
CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG 
CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC 
ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC 
CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC 
GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG 
ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC 
CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG 
TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT 
GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG 
GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC 
GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC 
AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC 
CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT 
ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG 
ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG 
GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC 
ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC 
GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG 
CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG 
ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC 
CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC 
ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC 
GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGA AGATCTCATC AATGACTGAG 
ACTGCCCCAA GGGCTCTGAC TACTGAAGTC CCCATCCCGG CCAAAATAAC AGTGACCATA 
GCCAACACAG AAACTTCTGA CATGCCCTTC TCTGCTGTTG ACATCCTGCA GCCCCCAGAG 
GAACTCCAGG TGTCGCCAGG TCCTCGCCAG CAGCTGCCGC CACGGCAGCT TCTGCAGTCG 
GCTTCCACAG CCCTGATGGG GGAGTCCGCC GAGGTCCTGT CAGCCTCCCA GACCCCTGAG 
CTCCCGGCCG CCGTGGATCT GAGCAGCACA GGGGAGCCAT CTTCGGGCCA GGAGTCTGCC 
GGCTCTGCGG TGGTGGCCAC TGTGGTGGTC CAGCCACCCC CACCCACACA GTCCGAAGTA 
GACCAGTTAT CACTTCCCCA AGAGCTAATG GCCGAGGCCC AAGCTGGCAC CACCACCCTC 
ATGGTAACGG GGCTCACCCC CGAGGAGCTG GCAGTGACGG CTGCTGCAGA AGCAGCTGCC 
CAGGCCGCAG CCACGGAGGA AGCCCAGGCC CTGGCCATCC AGGCGGTGCT CCAGGCCGCG 
CAGCAGGCCG TCATGGGCAC CGGCGAGCCC ATGGACACCT CCGAGGCAGC AGCAACCGTG 
ACTCAGGCGG AGCTGGGGCA CCTGTCGGCC GAGGGTCAGG AGGGCCAGGC CACCACCATA 
CCCATTGTGC TGACACAGCA GGAGCTGGCT GCCCTGGTGC AGCAGCAGCA GCTGCAGGAG 
GCCCAGGCCC AGCAGCAGCA TCACCACCTC CCCACTGAGG CCCTGGCCCC TGCCGACAGT 
CTCAACGACC CAGCCATTGA GAGCAATTGC CTCAATGAGC TGGCCGGCAC GGTCCCCAGC 
ACTGTGGCGC TGCTGCCCTC AACGGCCACT GAGAGCCTGG CTCCATCCAA CACATTTGTG 
GCCCCCCAGC CGGTTGTGGT GGCCAGCCCA GCCAAGCTGC AGGCTGCAGC TACCCTGACC 
GAAGTGGCCA ATGGCATCGA GTCCCTGGGT GTGAAGCCAG ACCTGCCGCC CCCACCCAGC 
AAAGCCCCCA TGAAGAAGGA AAACCAGTGG TTTGATGTGG GAGTCATTAA GGGCACCAAT 
GTAATGGTGA CACACTATTT CCTGCCACCA GATGATGCTG TCCCATCAGA CGATGATTTG 
GGCACCGTCC CTGACTATAA CCAGCTGAAG AAGCAGGAGC TGCAGCCAGG CACAGCCTAT 
AAGTTTCGTG TTGCCGGAAT CAATGCCTGT GGCCGGGGGC CCTTCAGCGA AATCTCAGCC 
TTTAAGACGT GCCTGCCTGG TTTCCCAGGG GCCCCTTGTG CCATTAAAAT CAGCAAAAGT 
CCGGATGGTG CTCACCTCAC CTGGGAGCCA CCCTCTGTGA CCTCCGGCAA GATTATCGAG 
TACTCCGTGT ACCTGGCCAT CCAGAGCTCA CAGGCTGGGG GCGAGCTCAA GAGCTCCACC 
CCGGCCCAGC TGGCCTTCAT GCGGGTGTAC TGCGGGCCCA GCCCCTCCTG CCTGGTGCAG 
TCCTCCAGCC TTTCCAACGC CCACATCGAC TACACCACCA AGCCCGCCAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGCTATGGC CCGGCCACAC AAGTGAGGTG GCTGCAGGAA 
ACCAGTAAAG ACAGCTCTGG CACCAAGCCA GCCAACAAGC GGCCCATGTC CTCTCCAGAA 
ATGAAATCTG CTCCAAAGAA ATCTAAGGCC GATGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884960.1
CDS	994..7035
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_017029471.1
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029471.1

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAG CAACCCTGCC 
ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC 
ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA 
GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC 
CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG 
GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG 
ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC 
TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC 
AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC 
ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG 
ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA 
GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG 
CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC 
CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG 
CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG 
ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG 
GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC 
ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC 
CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT 
AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT 
ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT 
GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC 
AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG 
CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG 
CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC 
ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC 
CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC 
GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG 
ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC 
CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG 
TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT 
GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG 
GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC 
GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC 
AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC 
CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT 
ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG 
ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG 
GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC 
ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC 
GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG 
CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG 
ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC 
CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC 
ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC 
GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGA AGATCTCATC AATGACTGAG 
ACTGCCCCAA GGGCTCTGAC TACTGAAGTC CCCATCCCGG CCAAAATAAC AGTGACCATA 
GCCAACACAG AAACTTCTGA CATGCCCTTC TCTGCTGTTG ACATCCTGCA GCCCCCAGAG 
GAACTCCAGG TGTCGCCAGG TCCTCGCCAG CAGCTGCCGC CACGGCAGCT TCTGCAGTCG 
GCTTCCACAG CCCTGATGGG GGAGTCCGCC GAGGTCCTGT CAGCCTCCCA GACCCCTGAG 
CTCCCGGCCG CCGTGGATCT GAGCAGCACA GGGGAGCCAT CTTCGGGCCA GGAGTCTGCC 
GGCTCTGCGG TGGTGGCCAC TGTGGTGGTC CAGCCACCCC CACCCACACA GTCCGAAGTA 
GACCAGTTAT CACTTCCCCA AGAGCTAATG GCCGAGGCCC AAGCTGGCAC CACCACCCTC 
ATGGTAACGG GGCTCACCCC CGAGGAGCTG GCAGTGACGG CTGCTGCAGA AGCAGCTGCC 
CAGGCCGCAG CCACGGAGGA AGCCCAGGCC CTGGCCATCC AGGCGGTGCT CCAGGCCGCG 
CAGCAGGCCG TCATGGGCAC CGGCGAGCCC ATGGACACCT CCGAGGCAGC AGCAACCGTG 
ACTCAGGCGG AGCTGGGGCA CCTGTCGGCC GAGGGTCAGG AGGGCCAGGC CACCACCATA 
CCCATTGTGC TGACACAGCA GGAGCTGGCT GCCCTGGTGC AGCAGCAGCA GCTGCAGGAG 
GCCCAGGCCC AGCAGCAGCA TCACCACCTC CCCACTGAGG CCCTGGCCCC TGCCGACAGT 
CTCAACGACC CAGCCATTGA GAGCAATTGC CTCAATGAGC TGGCCGGCAC GGTCCCCAGC 
ACTGTGGCGC TGCTGCCCTC AACGGCCACT GAGAGCCTGG CTCCATCCAA CACATTTGTG 
GCCCCCCAGC CGGTTGTGGT GGCCAGCCCA GCCAAGCTGC AGGCTGCAGC TACCCTGACC 
GAAGTGGCCA ATGGCATCGA GTCCCTGGGT GTGAAGCCAG ACCTGCCGCC CCCACCCAGC 
AAAGCCCCCA TGAAGAAGGA AAACCAGTGG TTTGATGTGG GAGTCATTAA GGGCACCAAT 
GTAATGGTGA CACACTATTT CCTGCCACCA GATGATGCTG TCCCATCAGA CGATGATTTG 
GGCACCGTCC CTGACTATAA CCAGCTGAAG AAGCAGGAGC TGCAGCCAGG CACAGCCTAT 
AAGTTTCGTG TTGCCGGAAT CAATGCCTGT GGCCGGGGGC CCTTCAGCGA AATCTCAGCC 
TTTAAGACGT GCCTGCCTGG TTTCCCAGGG GCCCCTTGTG CCATTAAAAT CAGCAAAAGT 
CCGGATGGTG CTCACCTCAC CTGGGAGCCA CCCTCTGTGA CCTCCGGCAA GATTATCGAG 
TACTCCGTGT ACCTGGCCAT CCAGAGCTCA CAGGCTGGGG GCGAGCTCAA GAGCTCCACC 
CCGGCCCAGC TGGCCTTCAT GCGGGTGTAC TGCGGGCCCA GCCCCTCCTG CCTGGTGCAG 
TCCTCCAGCC TTTCCAACGC CCACATCGAC TACACCACCA AGCCCGCCAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGCTATGGC CCGGCCACAC AAGTGAGGTG GCTGCAGGAA 
ACCAGTAAAG ACAGCTCTGG CACCAAGCCA GCCAACAAGC GGCCCATGTC CTCTCCAGAA 
ATGAAATCTG CTCCAAAGAA ATCTAAGGCC GATGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_017029471.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884960.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.

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CloneID	OHu99282
Clone ID Related Accession (Same CDS sequence)	XM_017029471.1 , XM_017029471.2
Accession Version	XM_017029471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAG CAACCCTGCC 
ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC 
ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA 
GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC 
CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG 
GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG 
ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC 
TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC 
AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC 
ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG 
ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA 
GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG 
CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC 
CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG 
CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG 
ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG 
GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC 
ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC 
CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT 
AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT 
ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT 
GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC 
AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG 
CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG 
CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC 
ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC 
CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC 
GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG 
ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC 
CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG 
TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT 
GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG 
GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC 
GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC 
AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC 
CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT 
ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG 
ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG 
GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC 
ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC 
GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG 
CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG 
ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC 
CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC 
ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC 
GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGA AGATCTCATC AATGACTGAG 
ACTGCCCCAA GGGCTCTGAC TACTGAAGTC CCCATCCCGG CCAAAATAAC AGTGACCATA 
GCCAACACAG AAACTTCTGA CATGCCCTTC TCTGCTGTTG ACATCCTGCA GCCCCCAGAG 
GAACTCCAGG TGTCGCCAGG TCCTCGCCAG CAGCTGCCGC CACGGCAGCT TCTGCAGTCG 
GCTTCCACAG CCCTGATGGG GGAGTCCGCC GAGGTCCTGT CAGCCTCCCA GACCCCTGAG 
CTCCCGGCCG CCGTGGATCT GAGCAGCACA GGGGAGCCAT CTTCGGGCCA GGAGTCTGCC 
GGCTCTGCGG TGGTGGCCAC TGTGGTGGTC CAGCCACCCC CACCCACACA GTCCGAAGTA 
GACCAGTTAT CACTTCCCCA AGAGCTAATG GCCGAGGCCC AAGCTGGCAC CACCACCCTC 
ATGGTAACGG GGCTCACCCC CGAGGAGCTG GCAGTGACGG CTGCTGCAGA AGCAGCTGCC 
CAGGCCGCAG CCACGGAGGA AGCCCAGGCC CTGGCCATCC AGGCGGTGCT CCAGGCCGCG 
CAGCAGGCCG TCATGGGCAC CGGCGAGCCC ATGGACACCT CCGAGGCAGC AGCAACCGTG 
ACTCAGGCGG AGCTGGGGCA CCTGTCGGCC GAGGGTCAGG AGGGCCAGGC CACCACCATA 
CCCATTGTGC TGACACAGCA GGAGCTGGCT GCCCTGGTGC AGCAGCAGCA GCTGCAGGAG 
GCCCAGGCCC AGCAGCAGCA TCACCACCTC CCCACTGAGG CCCTGGCCCC TGCCGACAGT 
CTCAACGACC CAGCCATTGA GAGCAATTGC CTCAATGAGC TGGCCGGCAC GGTCCCCAGC 
ACTGTGGCGC TGCTGCCCTC AACGGCCACT GAGAGCCTGG CTCCATCCAA CACATTTGTG 
GCCCCCCAGC CGGTTGTGGT GGCCAGCCCA GCCAAGCTGC AGGCTGCAGC TACCCTGACC 
GAAGTGGCCA ATGGCATCGA GTCCCTGGGT GTGAAGCCAG ACCTGCCGCC CCCACCCAGC 
AAAGCCCCCA TGAAGAAGGA AAACCAGTGG TTTGATGTGG GAGTCATTAA GGGCACCAAT 
GTAATGGTGA CACACTATTT CCTGCCACCA GATGATGCTG TCCCATCAGA CGATGATTTG 
GGCACCGTCC CTGACTATAA CCAGCTGAAG AAGCAGGAGC TGCAGCCAGG CACAGCCTAT 
AAGTTTCGTG TTGCCGGAAT CAATGCCTGT GGCCGGGGGC CCTTCAGCGA AATCTCAGCC 
TTTAAGACGT GCCTGCCTGG TTTCCCAGGG GCCCCTTGTG CCATTAAAAT CAGCAAAAGT 
CCGGATGGTG CTCACCTCAC CTGGGAGCCA CCCTCTGTGA CCTCCGGCAA GATTATCGAG 
TACTCCGTGT ACCTGGCCAT CCAGAGCTCA CAGGCTGGGG GCGAGCTCAA GAGCTCCACC 
CCGGCCCAGC TGGCCTTCAT GCGGGTGTAC TGCGGGCCCA GCCCCTCCTG CCTGGTGCAG 
TCCTCCAGCC TTTCCAACGC CCACATCGAC TACACCACCA AGCCCGCCAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGCTATGGC CCGGCCACAC AAGTGAGGTG GCTGCAGGAA 
ACCAGTAAAG ACAGCTCTGG CACCAAGCCA GCCAACAAGC GGCCCATGTC CTCTCCAGAA 
ATGAAATCTG CTCCAAAGAA ATCTAAGGCC GATGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884960.1
CDS	1521..7562
Translation	MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_017029471.2
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029471.2

ORF Insert Sequence:

ATGGCTTCGG CCGTGTCGCC CGCCAACTTG CCAGCGGTGC TTCTGCAGCC CCGCTGGAAG 
CGAGTGGTGG GCTGGTCGGG TCCGGTGCCA CGGCCCCGCC ACGGCCACCG CGCCGTGGCC 
ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GAATAGTGGA CGAACTGCAC 
GTGTACAACA CGGCAACCAA CCAGTGGTTC ATCCCAGCCG TGAGGGGGGA CATTCCCCCT 
GGGTGTGCAG CCTATGGCTT CGTGTGTGAC GGGACTCGCC TCCTGGTGTT TGGTGGGATG 
GTGGAGTATG GGAAATACAG CAATGACCTC TACGAACTCC AGGCGAGCCG GTGGGAGTGG 
AAGAGACTCA AAGCAAAGAC GCCCAAAAAC GGGCCCCCTC CGTGTCCTCG ACTCGGGCAC 
AGCTTCTCCC TTGTGGGCAA CAAATGCTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG 
GACCCAAAGA ACAACATTCC AAGGTACCTG AATGACTTAT ATATCCTGGA ATTACGGCCA 
GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GGGTCCTACC ACCACCCCGG 
GAGTCACATA CTGCCGTGGT CTACACCGAA AAAGACAATA AGAAGTCCAA GCTGGTGATC 
TACGGCGGGA TGAGTGGCTG CAGGCTGGGG GACCTGTGGA CCCTAGATAT TGACACCCTG 
ACGTGGAATA AGCCCAGTCT CAGCGGGGTG GCGCCTCTTC CTCGCAGTCT CCACTCGGCA 
ACCACCATCG GAAATAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC 
GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACGCTGGC TTGTCTCAAC 
CTGGATACCA TGGCCTGGGA GACCATCCTG ATGGATACAC TGGAGGACAA CATCCCCCGT 
GCTCGGGCTG GCCACTGCGC AGTCGCCATC AACACCCGCC TGTACATTTG GAGTGGGCGT 
GACGGCTACC GCAAGGCCTG GAACAACCAG GTCTGCTGCA AGGACCTCTG GTACCTAGAG 
ACAGAAAAGC CACCACCCCC AGCCCGAGTA CAACTGGTAC GCGCCAACAC CAACTCCCTG 
GAGGTGAGCT GGGGGGCAGT GGCAACAGCC GACAGCTACC TTCTCCAGCT CCAGAAATAT 
GACATTCCTG CCACGGCTGC TACTGCCACC TCCCCTACAC CCAATCCGGT CCCATCTGTG 
CCTGCCAACC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCTGT GCAGCCGCTG 
ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCCCCCG CACCCCCGAC CACCACCACC 
ATCCAGGTCT TGCCAACGGT GCCTGGCAGC TCCATTTCTG TGCCCACCGC AGCCAGGACT 
CAAGGTGTCC CTGCTGTTCT CAAAGTGACC GGTCCTCAGG CTACAACAGG AACTCCATTG 
GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCTG TCACCGTGAC CTCCCTTCCC 
GCCGGAGTGC GGATGGTTGT GCCAACACAG AGTGCCCAGG GAACGGTGAG CAACCCTGCC 
ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC 
ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA 
GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC 
CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG 
GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG 
ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC 
TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC 
AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC 
ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG 
ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA 
GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG 
CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC 
CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG 
CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG 
ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG 
GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC 
ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC 
CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT 
AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT 
ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT 
GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC 
AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG 
CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG 
CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC 
ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC 
CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC 
GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG 
ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC 
CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG 
TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT 
GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG 
GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC 
GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC 
AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC 
CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT 
ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG 
ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG 
GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC 
ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC 
GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG 
CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG 
ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC 
CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC 
ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC 
GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGA AGATCTCATC AATGACTGAG 
ACTGCCCCAA GGGCTCTGAC TACTGAAGTC CCCATCCCGG CCAAAATAAC AGTGACCATA 
GCCAACACAG AAACTTCTGA CATGCCCTTC TCTGCTGTTG ACATCCTGCA GCCCCCAGAG 
GAACTCCAGG TGTCGCCAGG TCCTCGCCAG CAGCTGCCGC CACGGCAGCT TCTGCAGTCG 
GCTTCCACAG CCCTGATGGG GGAGTCCGCC GAGGTCCTGT CAGCCTCCCA GACCCCTGAG 
CTCCCGGCCG CCGTGGATCT GAGCAGCACA GGGGAGCCAT CTTCGGGCCA GGAGTCTGCC 
GGCTCTGCGG TGGTGGCCAC TGTGGTGGTC CAGCCACCCC CACCCACACA GTCCGAAGTA 
GACCAGTTAT CACTTCCCCA AGAGCTAATG GCCGAGGCCC AAGCTGGCAC CACCACCCTC 
ATGGTAACGG GGCTCACCCC CGAGGAGCTG GCAGTGACGG CTGCTGCAGA AGCAGCTGCC 
CAGGCCGCAG CCACGGAGGA AGCCCAGGCC CTGGCCATCC AGGCGGTGCT CCAGGCCGCG 
CAGCAGGCCG TCATGGGCAC CGGCGAGCCC ATGGACACCT CCGAGGCAGC AGCAACCGTG 
ACTCAGGCGG AGCTGGGGCA CCTGTCGGCC GAGGGTCAGG AGGGCCAGGC CACCACCATA 
CCCATTGTGC TGACACAGCA GGAGCTGGCT GCCCTGGTGC AGCAGCAGCA GCTGCAGGAG 
GCCCAGGCCC AGCAGCAGCA TCACCACCTC CCCACTGAGG CCCTGGCCCC TGCCGACAGT 
CTCAACGACC CAGCCATTGA GAGCAATTGC CTCAATGAGC TGGCCGGCAC GGTCCCCAGC 
ACTGTGGCGC TGCTGCCCTC AACGGCCACT GAGAGCCTGG CTCCATCCAA CACATTTGTG 
GCCCCCCAGC CGGTTGTGGT GGCCAGCCCA GCCAAGCTGC AGGCTGCAGC TACCCTGACC 
GAAGTGGCCA ATGGCATCGA GTCCCTGGGT GTGAAGCCAG ACCTGCCGCC CCCACCCAGC 
AAAGCCCCCA TGAAGAAGGA AAACCAGTGG TTTGATGTGG GAGTCATTAA GGGCACCAAT 
GTAATGGTGA CACACTATTT CCTGCCACCA GATGATGCTG TCCCATCAGA CGATGATTTG 
GGCACCGTCC CTGACTATAA CCAGCTGAAG AAGCAGGAGC TGCAGCCAGG CACAGCCTAT 
AAGTTTCGTG TTGCCGGAAT CAATGCCTGT GGCCGGGGGC CCTTCAGCGA AATCTCAGCC 
TTTAAGACGT GCCTGCCTGG TTTCCCAGGG GCCCCTTGTG CCATTAAAAT CAGCAAAAGT 
CCGGATGGTG CTCACCTCAC CTGGGAGCCA CCCTCTGTGA CCTCCGGCAA GATTATCGAG 
TACTCCGTGT ACCTGGCCAT CCAGAGCTCA CAGGCTGGGG GCGAGCTCAA GAGCTCCACC 
CCGGCCCAGC TGGCCTTCAT GCGGGTGTAC TGCGGGCCCA GCCCCTCCTG CCTGGTGCAG 
TCCTCCAGCC TTTCCAACGC CCACATCGAC TACACCACCA AGCCCGCCAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGCTATGGC CCGGCCACAC AAGTGAGGTG GCTGCAGGAA 
ACCAGTAAAG ACAGCTCTGG CACCAAGCCA GCCAACAAGC GGCCCATGTC CTCTCCAGAA 
ATGAAATCTG CTCCAAAGAA ATCTAAGGCC GATGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCFC1 ( XM_017029472.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884961.1 Homo sapiens host cell factor C1 (HCFC1), transcript variant X6, mRNA.

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CloneID	OHu99283
Clone ID Related Accession (Same CDS sequence)	XM_017029472.1
Accession Version	XM_017029472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	host cell factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG AGACCATCCT GATGGATACA CTGGAGGACA ACATCCCCCG TGCTCGGGCT 
GGCCACTGCG CAGTCGCCAT CAACACCCGC CTGTACATTT GGAGTGGGCG TGACGGCTAC 
CGCAAGGCCT GGAACAACCA GGTCTGCTGC AAGGACCTCT GGTACCTAGA GACAGAAAAG 
CCACCACCCC CAGCCCGAGT ACAACTGGTA CGCGCCAACA CCAACTCCCT GGAGGTGAGC 
TGGGGGGCAG TGGCAACAGC CGACAGCTAC CTTCTCCAGC TCCAGAAATA TGACATTCCT 
GCCACGGCTG CTACTGCCAC CTCCCCTACA CCCAATCCGG TCCCATCTGT GCCTGCCAAC 
CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCTG TGCAGCCGCT GACCCAAGTA 
GGCATCACGC TCCTGCCCCA GGCTGCCCCC GCACCCCCGA CCACCACCAC CATCCAGGTC 
TTGCCAACGG TGCCTGGCAG CTCCATTTCT GTGCCCACCG CAGCCAGGAC TCAAGGTGTC 
CCTGCTGTTC TCAAAGTGAC CGGTCCTCAG GCTACAACAG GAACTCCATT GGTCACCATG 
CGACCTGCCA GCCAGGCTGG GAAAGCCCCT GTCACCGTGA CCTCCCTTCC CGCCGGAGTG 
CGGATGGTTG TGCCAACACA GAGTGCCCAG GGAACGGTGA TTGGCAGTAG CCCACAGATG 
AGTGGGATGG CCGCACTGGC CGCTGCGGCC GCTGCCACCC AGAAGATCCC CCCTTCCTCG 
GCACCCACGG TGCTGAGTGT CCCAGCGGGT ACCACCATCG TGAAGACCAT GGCTGTGACA 
CCTGGCACTA CCACCCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC 
AACCCTGCCA CTCGCATGCT GAAGACTGCA GCCGCCCAGG TGGGGACATC GGTTTCCTCC 
GCCACCAACA CGTCTACCCG CCCTATCATC ACAGTGCACA AGTCAGGCAC TGTGACAGTG 
GCCCAGCAAG CCCAGGTGGT GACCACAGTT GTGGGCGGGG TCACCAAGAC CATCACCCTG 
GTGAAGAGCC CCATCTCTGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTG 
ATGTCGGTGG TCCAGACCAA ACCAGTTCAG ACTTCAGCAG TCACAGGCCA GGCGTCCACG 
GGTCCTGTGA CTCAGATCAT CCAGACCAAA GGGCCCCTGC CAGCGGGAAC AATCCTGAAG 
CTGGTGACCT CAGCAGATGG CAAGCCCACC ACCATCATCA CTACCACGCA GGCCAGTGGG 
GCGGGGACCA AGCCCACCAT CCTGGGCATC AGCAGCGTCT CCCCCAGTAC CACCAAGCCC 
GGCACGACCA CCATCATCAA AACCATCCCC ATGTCGGCCA TCATCACCCA GGCGGGCGCC 
ACGGGTGTGA CCAGCAGTCC TGGCATCAAG TCCCCCATCA CCATCATCAC CACCAAGGTG 
ATGACTTCAG GAACTGGAGC ACCTGCGAAA ATCATCACTG CTGTCCCCAA AATTGCCACT 
GGCCACGGGC AGCAGGGAGT GACCCAGGTG GTGCTTAAGG GGGCCCCGGG ACAGCCAGGC 
ACCATCCTCC GCACTGTGCC CATGGGGGGT GTTCGCCTGG TCACACCCGT CACCGTCTCC 
GCCGTCAAGC CAGCCGTCAC CACGTTGGTT GTGAAAGGCA CCACAGGTGT CACGACCCTA 
GGCACAGTGA CAGGCACCGT CTCCACCAGC CTTGCCGGGG CGGGGGGCCA CAGCACTAGT 
GCTTCCCTGG CCACGCCCAT CACCACCTTG GGCACCATTG CCACCCTCTC AAGCCAGGTG 
ATCAACCCCA CTGCCATCAC TGTGTCGGCC GCACAGACCA CGCTGACAGC GGCAGGCGGG 
CTCACAACCC CAACCATCAC CATGCAGCCC GTGTCCCAGC CCACCCAGGT AACTCTGATC 
ACGGCACCTA GTGGGGTGGA GGCCCAGCCT GTGCATGACC TCCCTGTGTC CATTCTGGCC 
TCCCCGACTA CAGAACAGCC CACCGCCACA GTTACCATCG CCGACTCAGG CCAGGGTGAT 
GTGCAGCCTG GCACTGTCAC CTTGGTGTGC TCCAACCCAC CCTGTGAGAC CCACGAGACT 
GGCACCACCA ACACGGCCAC CACTACTGTT GTGGCTAACC TTGGGGGACA CCCCCAGCCC 
ACCCAAGTGC AGTTCGTCTG TGACAGACAG GAGGCAGCTG CTTCTCTTGT GACCTCGACT 
GTGGGCCAGC AGAATGGTAG CGTGGTCCGA GTCTGTTCGA ACCCGCCCTG CGAGACCCAC 
GAGACGGGCA CCACCAACAC CGCCACCACC GCCACCTCCA ACATGGCCGG GCAGCATGGC 
TGCTCAAACC CACCCTGCGA GACCCACGAG ACGGGCACCA CCAACACTGC CACTACAGCC 
ATGTCGAGCG TCGGCGCCAA CCACCAGCGA GATGCCCGTC GGGCCTGTGC AGCTGGCACC 
CCTGCCGTGA TCCGGATCAG TGTGGCCACT GGGGCGCTGG AGGCAGCCCA GGGCTCTAAG 
TCCCAGTGCC AAACCCGCCA GACCAGCGCG ACCAGCACCA CCATGACTGT GATGGCCACC 
GGGGCCCCGT GCTCGGCCGG CCCACTCCTT GGGCCGAGCA TGGCACGGGA GCCCGGGGGC 
CGCAGCCCTG CTTTTGTGCA GTTGGCCCCT CTGAGCAGCA AAGTCAGGCT GAGCAGCCCA 
AGCATTAAGG ACCTTCCTGC GGGGCGCCAC AGCCATGCGG TCAGCACCGC TGCCATGACC 
CGTTCCAGCG TGGGTGCTGG GGAGCCCCGC ATGGCACCTG TGTGCGAGAG CCTCCAGGGT 
GGCTCGCCCA GCACCACAGT GACTGTGACA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AAGTCTGCTC CAACCCACCA TGTGAGACCC ACGAGACAGG CACCACCAAC 
ACCGCCACTA CCTCGAATGC AGGCAGCGCC CAGAGGGTGT GCTCCAACCC GCCATGCGAG 
ACCCACGAGA CGGGCACCAC CCACACGGCC ACCACCGCTA CTTCAAACGG GGGCACGGGC 
CAGCCCGAGG GTGGGCAGCA GCCCCCTGCT GGTCGCCCCT GTGAGACACA CCAGACCACT 
TCCACTGGCA CCACCATGTC GGTCAGCGTG GGTGCCCTGC TTCCCGACGC CACTTCTTCC 
CACAGGACCG TGGAGTCTGG CCTAGAGGTG GCGGCGGCAC CCAGCGTCAC CCCCCAGGCT 
GGCACCGCGC TGCTGGCTCC TTTCCCAACA CAGAGGGTGT GCTCCAACCC CCCCTGTGAG 
ACCCACGAGA CGGGCACCAC TCACACGGCC ACCACTGTCA CTTCCAACAT GAGTTCAAAC 
CAAGACCCCC CACCTGCTGC CAGCGATCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGC 
GTGAACATCA CCAGCTCCAG TGCCATCACG ACAACCGTGT CCTCCACACT GACGCGGGCT 
GTGACCACCG TGACGCAGTC CACACCGGTC CCGGGCCCCT CTGTGCCGAA GATCTCATCA 
ATGACTGAGA CTGCCCCAAG GGCTCTGACT ACTGAAGTCC CCATCCCGGC CAAAATAACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AACTCCAGGT GTCGCCAGGT CCTCGCCAGC AGCTGCCGCC ACGGCAGCTT 
CTGCAGTCGG CTTCCACAGC CCTGATGGGG GAGTCCGCCG AGGTCCTGTC AGCCTCCCAG 
ACCCCTGAGC TCCCGGCCGC CGTGGATCTG AGCAGCACAG GGGAGCCATC TTCGGGCCAG 
GAGTCTGCCG GCTCTGCGGT GGTGGCCACT GTGGTGGTCC AGCCACCCCC ACCCACACAG 
TCCGAAGTAG ACCAGTTATC ACTTCCCCAA GAGCTAATGG CCGAGGCCCA AGCTGGCACC 
ACCACCCTCA TGGTAACGGG GCTCACCCCC GAGGAGCTGG CAGTGACGGC TGCTGCAGAA 
GCAGCTGCCC AGGCCGCAGC CACGGAGGAA GCCCAGGCCC TGGCCATCCA GGCGGTGCTC 
CAGGCCGCGC AGCAGGCCGT CATGGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGAAGCCAGA CCTGCCGCCC 
CCACCCAGCA AAGCCCCCAT GAAGAAGGAA AACCAGTGGT TTGATGTGGG AGTCATTAAG 
GGCACCAATG TAATGGTGAC ACACTATTTC CTGCCACCAG ATGATGCTGT CCCATCAGAC 
GATGATTTGG GCACCGTCCC TGACTATAAC CAGCTGAAGA AGCAGGAGCT GCAGCCAGGC 
ACAGCCTATA AGTTTCGTGT TGCCGGAATC AATGCCTGTG GCCGGGGGCC CTTCAGCGAA 
ATCTCAGCCT TTAAGACGTG CCTGCCTGGT TTCCCAGGGG CCCCTTGTGC CATTAAAATC 
AGCAAAAGTC CGGATGGTGC TCACCTCACC TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG 
ATTATCGAGT ACTCCGTGTA CCTGGCCATC CAGAGCTCAC AGGCTGGGGG CGAGCTCAAG 
AGCTCCACCC CGGCCCAGCT GGCCTTCATG CGGGTGTACT GCGGGCCCAG CCCCTCCTGC 
CTGGTGCAGT CCTCCAGCCT TTCCAACGCC CACATCGACT ACACCACCAA GCCCGCCATC 
ATCTTCCGCA TCGCCGCCCG CAATGAGAAG GGCTATGGCC CGGCCACACA AGTGAGGTGG 
CTGCAGGAAA CCAGTAAAGA CAGCTCTGGC ACCAAGCCAG CCAACAAGCG GCCCATGTCC 
TCTCCAGAAA TGAAATCTGC TCCAAAGAAA TCTAAGGCCG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884961.1
CDS	91..5421
Translation	MAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETAPRALTTEVPIPAKITVTIANTETSDMPFSAVDILQPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ

Target ORF information:

RefSeq Version	XM_017029472.1
Organism	Homo sapiens(human)
Definition	Homo sapiens host cell factor C1 (HCFC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029472.1

ORF Insert Sequence:

ATGGCCTGGG AGACCATCCT GATGGATACA CTGGAGGACA ACATCCCCCG TGCTCGGGCT 
GGCCACTGCG CAGTCGCCAT CAACACCCGC CTGTACATTT GGAGTGGGCG TGACGGCTAC 
CGCAAGGCCT GGAACAACCA GGTCTGCTGC AAGGACCTCT GGTACCTAGA GACAGAAAAG 
CCACCACCCC CAGCCCGAGT ACAACTGGTA CGCGCCAACA CCAACTCCCT GGAGGTGAGC 
TGGGGGGCAG TGGCAACAGC CGACAGCTAC CTTCTCCAGC TCCAGAAATA TGACATTCCT 
GCCACGGCTG CTACTGCCAC CTCCCCTACA CCCAATCCGG TCCCATCTGT GCCTGCCAAC 
CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCTG TGCAGCCGCT GACCCAAGTA 
GGCATCACGC TCCTGCCCCA GGCTGCCCCC GCACCCCCGA CCACCACCAC CATCCAGGTC 
TTGCCAACGG TGCCTGGCAG CTCCATTTCT GTGCCCACCG CAGCCAGGAC TCAAGGTGTC 
CCTGCTGTTC TCAAAGTGAC CGGTCCTCAG GCTACAACAG GAACTCCATT GGTCACCATG 
CGACCTGCCA GCCAGGCTGG GAAAGCCCCT GTCACCGTGA CCTCCCTTCC CGCCGGAGTG 
CGGATGGTTG TGCCAACACA GAGTGCCCAG GGAACGGTGA TTGGCAGTAG CCCACAGATG 
AGTGGGATGG CCGCACTGGC CGCTGCGGCC GCTGCCACCC AGAAGATCCC CCCTTCCTCG 
GCACCCACGG TGCTGAGTGT CCCAGCGGGT ACCACCATCG TGAAGACCAT GGCTGTGACA 
CCTGGCACTA CCACCCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC 
AACCCTGCCA CTCGCATGCT GAAGACTGCA GCCGCCCAGG TGGGGACATC GGTTTCCTCC 
GCCACCAACA CGTCTACCCG CCCTATCATC ACAGTGCACA AGTCAGGCAC TGTGACAGTG 
GCCCAGCAAG CCCAGGTGGT GACCACAGTT GTGGGCGGGG TCACCAAGAC CATCACCCTG 
GTGAAGAGCC CCATCTCTGT CCCAGGAGGC AGTGCTCTGA TTTCCAATCT GGGCAAAGTG 
ATGTCGGTGG TCCAGACCAA ACCAGTTCAG ACTTCAGCAG TCACAGGCCA GGCGTCCACG 
GGTCCTGTGA CTCAGATCAT CCAGACCAAA GGGCCCCTGC CAGCGGGAAC AATCCTGAAG 
CTGGTGACCT CAGCAGATGG CAAGCCCACC ACCATCATCA CTACCACGCA GGCCAGTGGG 
GCGGGGACCA AGCCCACCAT CCTGGGCATC AGCAGCGTCT CCCCCAGTAC CACCAAGCCC 
GGCACGACCA CCATCATCAA AACCATCCCC ATGTCGGCCA TCATCACCCA GGCGGGCGCC 
ACGGGTGTGA CCAGCAGTCC TGGCATCAAG TCCCCCATCA CCATCATCAC CACCAAGGTG 
ATGACTTCAG GAACTGGAGC ACCTGCGAAA ATCATCACTG CTGTCCCCAA AATTGCCACT 
GGCCACGGGC AGCAGGGAGT GACCCAGGTG GTGCTTAAGG GGGCCCCGGG ACAGCCAGGC 
ACCATCCTCC GCACTGTGCC CATGGGGGGT GTTCGCCTGG TCACACCCGT CACCGTCTCC 
GCCGTCAAGC CAGCCGTCAC CACGTTGGTT GTGAAAGGCA CCACAGGTGT CACGACCCTA 
GGCACAGTGA CAGGCACCGT CTCCACCAGC CTTGCCGGGG CGGGGGGCCA CAGCACTAGT 
GCTTCCCTGG CCACGCCCAT CACCACCTTG GGCACCATTG CCACCCTCTC AAGCCAGGTG 
ATCAACCCCA CTGCCATCAC TGTGTCGGCC GCACAGACCA CGCTGACAGC GGCAGGCGGG 
CTCACAACCC CAACCATCAC CATGCAGCCC GTGTCCCAGC CCACCCAGGT AACTCTGATC 
ACGGCACCTA GTGGGGTGGA GGCCCAGCCT GTGCATGACC TCCCTGTGTC CATTCTGGCC 
TCCCCGACTA CAGAACAGCC CACCGCCACA GTTACCATCG CCGACTCAGG CCAGGGTGAT 
GTGCAGCCTG GCACTGTCAC CTTGGTGTGC TCCAACCCAC CCTGTGAGAC CCACGAGACT 
GGCACCACCA ACACGGCCAC CACTACTGTT GTGGCTAACC TTGGGGGACA CCCCCAGCCC 
ACCCAAGTGC AGTTCGTCTG TGACAGACAG GAGGCAGCTG CTTCTCTTGT GACCTCGACT 
GTGGGCCAGC AGAATGGTAG CGTGGTCCGA GTCTGTTCGA ACCCGCCCTG CGAGACCCAC 
GAGACGGGCA CCACCAACAC CGCCACCACC GCCACCTCCA ACATGGCCGG GCAGCATGGC 
TGCTCAAACC CACCCTGCGA GACCCACGAG ACGGGCACCA CCAACACTGC CACTACAGCC 
ATGTCGAGCG TCGGCGCCAA CCACCAGCGA GATGCCCGTC GGGCCTGTGC AGCTGGCACC 
CCTGCCGTGA TCCGGATCAG TGTGGCCACT GGGGCGCTGG AGGCAGCCCA GGGCTCTAAG 
TCCCAGTGCC AAACCCGCCA GACCAGCGCG ACCAGCACCA CCATGACTGT GATGGCCACC 
GGGGCCCCGT GCTCGGCCGG CCCACTCCTT GGGCCGAGCA TGGCACGGGA GCCCGGGGGC 
CGCAGCCCTG CTTTTGTGCA GTTGGCCCCT CTGAGCAGCA AAGTCAGGCT GAGCAGCCCA 
AGCATTAAGG ACCTTCCTGC GGGGCGCCAC AGCCATGCGG TCAGCACCGC TGCCATGACC 
CGTTCCAGCG TGGGTGCTGG GGAGCCCCGC ATGGCACCTG TGTGCGAGAG CCTCCAGGGT 
GGCTCGCCCA GCACCACAGT GACTGTGACA GCCCTGGAGG CACTGCTGTG CCCCTCGGCC 
ACCGTGACCC AAGTCTGCTC CAACCCACCA TGTGAGACCC ACGAGACAGG CACCACCAAC 
ACCGCCACTA CCTCGAATGC AGGCAGCGCC CAGAGGGTGT GCTCCAACCC GCCATGCGAG 
ACCCACGAGA CGGGCACCAC CCACACGGCC ACCACCGCTA CTTCAAACGG GGGCACGGGC 
CAGCCCGAGG GTGGGCAGCA GCCCCCTGCT GGTCGCCCCT GTGAGACACA CCAGACCACT 
TCCACTGGCA CCACCATGTC GGTCAGCGTG GGTGCCCTGC TTCCCGACGC CACTTCTTCC 
CACAGGACCG TGGAGTCTGG CCTAGAGGTG GCGGCGGCAC CCAGCGTCAC CCCCCAGGCT 
GGCACCGCGC TGCTGGCTCC TTTCCCAACA CAGAGGGTGT GCTCCAACCC CCCCTGTGAG 
ACCCACGAGA CGGGCACCAC TCACACGGCC ACCACTGTCA CTTCCAACAT GAGTTCAAAC 
CAAGACCCCC CACCTGCTGC CAGCGATCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGC 
GTGAACATCA CCAGCTCCAG TGCCATCACG ACAACCGTGT CCTCCACACT GACGCGGGCT 
GTGACCACCG TGACGCAGTC CACACCGGTC CCGGGCCCCT CTGTGCCGAA GATCTCATCA 
ATGACTGAGA CTGCCCCAAG GGCTCTGACT ACTGAAGTCC CCATCCCGGC CAAAATAACA 
GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG 
CCCCCAGAGG AACTCCAGGT GTCGCCAGGT CCTCGCCAGC AGCTGCCGCC ACGGCAGCTT 
CTGCAGTCGG CTTCCACAGC CCTGATGGGG GAGTCCGCCG AGGTCCTGTC AGCCTCCCAG 
ACCCCTGAGC TCCCGGCCGC CGTGGATCTG AGCAGCACAG GGGAGCCATC TTCGGGCCAG 
GAGTCTGCCG GCTCTGCGGT GGTGGCCACT GTGGTGGTCC AGCCACCCCC ACCCACACAG 
TCCGAAGTAG ACCAGTTATC ACTTCCCCAA GAGCTAATGG CCGAGGCCCA AGCTGGCACC 
ACCACCCTCA TGGTAACGGG GCTCACCCCC GAGGAGCTGG CAGTGACGGC TGCTGCAGAA 
GCAGCTGCCC AGGCCGCAGC CACGGAGGAA GCCCAGGCCC TGGCCATCCA GGCGGTGCTC 
CAGGCCGCGC AGCAGGCCGT CATGGGCACC GGCGAGCCCA TGGACACCTC CGAGGCAGCA 
GCAACCGTGA CTCAGGCGGA GCTGGGGCAC CTGTCGGCCG AGGGTCAGGA GGGCCAGGCC 
ACCACCATAC CCATTGTGCT GACACAGCAG GAGCTGGCTG CCCTGGTGCA GCAGCAGCAG 
CTGCAGGAGG CCCAGGCCCA GCAGCAGCAT CACCACCTCC CCACTGAGGC CCTGGCCCCT 
GCCGACAGTC TCAACGACCC AGCCATTGAG AGCAATTGCC TCAATGAGCT GGCCGGCACG 
GTCCCCAGCA CTGTGGCGCT GCTGCCCTCA ACGGCCACTG AGAGCCTGGC TCCATCCAAC 
ACATTTGTGG CCCCCCAGCC GGTTGTGGTG GCCAGCCCAG CCAAGCTGCA GGCTGCAGCT 
ACCCTGACCG AAGTGGCCAA TGGCATCGAG TCCCTGGGTG TGAAGCCAGA CCTGCCGCCC 
CCACCCAGCA AAGCCCCCAT GAAGAAGGAA AACCAGTGGT TTGATGTGGG AGTCATTAAG 
GGCACCAATG TAATGGTGAC ACACTATTTC CTGCCACCAG ATGATGCTGT CCCATCAGAC 
GATGATTTGG GCACCGTCCC TGACTATAAC CAGCTGAAGA AGCAGGAGCT GCAGCCAGGC 
ACAGCCTATA AGTTTCGTGT TGCCGGAATC AATGCCTGTG GCCGGGGGCC CTTCAGCGAA 
ATCTCAGCCT TTAAGACGTG CCTGCCTGGT TTCCCAGGGG CCCCTTGTGC CATTAAAATC 
AGCAAAAGTC CGGATGGTGC TCACCTCACC TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG 
ATTATCGAGT ACTCCGTGTA CCTGGCCATC CAGAGCTCAC AGGCTGGGGG CGAGCTCAAG 
AGCTCCACCC CGGCCCAGCT GGCCTTCATG CGGGTGTACT GCGGGCCCAG CCCCTCCTGC 
CTGGTGCAGT CCTCCAGCCT TTCCAACGCC CACATCGACT ACACCACCAA GCCCGCCATC 
ATCTTCCGCA TCGCCGCCCG CAATGAGAAG GGCTATGGCC CGGCCACACA AGTGAGGTGG 
CTGCAGGAAA CCAGTAAAGA CAGCTCTGGC ACCAAGCCAG CCAACAAGCG GCCCATGTCC 
TCTCCAGAAA TGAAATCTGC TCCAAAGAAA TCTAAGGCCG ATGGTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HCFC1
Entrez Gene ID	3054
Full Name	host cell factor C1
Synonyms	CFF,HCF,HCF-1,HCF1,HFC1,MRX3,PPP1R89,VCAF
General protein information	Preferred Names host cell factor C1 Names host cell factor 1 VP16-accessory protein protein phosphatase 1, regulatory subunit 89
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	300019
Disorder Html:	Mental retardation, X-linked 3 (methylmalonic acidemia and homocysteinemia, cblX type ), 309541 (3)

Pan troglodytes (chimpanzee)	HCFC1	XP_521330.3
Rattus norvegicus (Norway rat)	Hcfc1	NP_001132979.1
Mus musculus (house mouse)	Hcfc1	NP_032250.2
Danio rerio (zebrafish)	hcfc1b	NP_001122009.1
Bos taurus (cattle)	HCFC1	XP_002707954.1
Homo sapiens (human)	HCFC1	NP_005325.2
Canis lupus familiaris (dog)	HCFC1	XP_005642062.1
Caenorhabditis elegans (roundworm)	hcf-1	NP_501279.1

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HCFC1 cDNA ORF clone, Homo sapiens(human)

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