HBG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBG1
Entrez Gene ID	3047
Full Name	hemoglobin subunit gamma 1
Synonyms	HBG-T2,HBGA,HBGR,HSGGL1,PRO2979
General protein information	Preferred Names hemoglobin subunit gamma 1 Names hemoglobin subunit gamma-1 A-gamma globin gamma A hemoglobin gamma globin gamma-1-globin hb F Agamma hemoglobin gamma-1 chain hemoglobin gamma-a chain hemoglobin, gamma A hemoglobin, gamma, regulator of
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008].
Disorder MIM:	142200
Disorder Html:	Fetal hemoglobin quantitative trait locus 1, 141749 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000559.2	NP_000550.2	hemoglobin subunit gamma-1
NM_000559.3	NP_000550.2	hemoglobin subunit gamma-1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hbe2	NP_001019976.1
Homo sapiens (human)	HBG1	NP_000550.2

Gene Ontology
Bibliography

Related articles in PubMed

An A?-globin G->A gene polymorphism associated with ?
Breveglieri G, Bianchi N, Cosenza LC, Gamberini MR, Chiavilli F, Zuccato C, Montagner G, Borgatti M, Lampronti I, Finotti A, Gambari R
BMC medical genetics18(1)93(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study describes the characterization of the rs368698783 (+25 G->A) polymorphism of the Agamma-globin gene associated in beta(0)39 thalassemia patients with high HbF in erythroid precursor cells
Title: An A?-globin G->A gene polymorphism associated with ?

Introduction of the British HPFH mutation into the fetal globin promoter in a human cell model causes elevated fetal globin expression. The British HPFH mutation creates a de novo binding site both in vitro and in vivo for the potent erythroid activator KLF1.
Title: KLF1 drives the expression of fetal hemoglobin in British HPFH.

A Ly1 antibody reactive (LYAR)-binding motif disruptive regulatory single-nucleotide polymorphisms rs368698783 (G/A) from LD block 5 in the proximal promoter of hemoglobin subunit gamma 1 (HBG1) was found to be a significant predictor for beta-thalassemia clinical severity.
Title: A Genetic Variant Ameliorates ?-Thalassemia Severity by Epigenetic-Mediated Elevation of Human Fetal Hemoglobin Expression.

Study found a novel polymorphism of the A-gamma-globin gene in four families with b0-thalassemia and high levels of HbF expression. Additionally, evidence suggesting that the Ac-globin gene +25(G-A) polymorphism decreases the efficiency of the interaction between this sequence and specific DNA binding protein complexes.
Title: Structural and Functional Insights on an Uncharacterized A?-Globin-Gene Polymorphism Present in Four ?0-Thalassemia Families with High Fetal Hemoglobin Levels.

results suggest that KLF1 directly regulates the beta-globin gene, but probably has less direct impact on expression of the gamma-globin gene in fetal erythroblasts
Title: Kr?ppel-Like Transcription Factor KLF1 Is Required for Optimal ?- and ?-Globin Expression in Human Fetal Erythroblasts.

The following HBG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25692	NM_000559.3 Latest version!	Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25692	NM_000559.2	Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBG1 ( NM_000559.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000550.2 Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.

Price & Availability↑

CloneID	OHu25692
Clone ID Related Accession (Same CDS sequence)	NM_000559.2 , NM_000559.3
Accession Version	NM_000559.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-13
Organism	Homo sapiens(human)
Product	hemoglobin subunit gamma-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M91036.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2003 this sequence version replaced NM_000559.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD238302.1, BG573862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCATT TCACAGAGGA GGACAAGGCT ACTATCACAA GCCTGTGGGG CAAGGTGAAT 
GTGGAAGATG CTGGAGGAGA AACCCTGGGA AGGCTCCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTTG ACAGCTTTGG CAACCTGTCC TCTGCCTCTG CCATCATGGG CAACCCCAAA 
GTCAAGGCAC ATGGCAAGAA GGTGCTGACT TCCTTGGGAG ATGCCACAAA GCACCTGGAT 
GATCTCAAGG GCACCTTTGC CCAGCTGAGT GAACTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGAAATGTG CTGGTGACCG TTTTGGCAAT CCATTTCGGC 
AAAGAATTCA CCCCTGAGGT GCAGGCTTCC TGGCAGAAGA TGGTGACTGC AGTGGCCAGT 
GCCCTGTCCT CCAGATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000550.2
CDS	54..497
Translation	MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDATKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTAVASALSSRYH

Target ORF information:

RefSeq Version	NM_000559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000559.2

ORF Insert Sequence:

ATGGGTCATT TCACAGAGGA GGACAAGGCT ACTATCACAA GCCTGTGGGG CAAGGTGAAT 
GTGGAAGATG CTGGAGGAGA AACCCTGGGA AGGCTCCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTTG ACAGCTTTGG CAACCTGTCC TCTGCCTCTG CCATCATGGG CAACCCCAAA 
GTCAAGGCAC ATGGCAAGAA GGTGCTGACT TCCTTGGGAG ATGCCACAAA GCACCTGGAT 
GATCTCAAGG GCACCTTTGC CCAGCTGAGT GAACTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGAAATGTG CTGGTGACCG TTTTGGCAAT CCATTTCGGC 
AAAGAATTCA CCCCTGAGGT GCAGGCTTCC TGGCAGAAGA TGGTGACTGC AGTGGCCAGT 
GCCCTGTCCT CCAGATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBG1 ( NM_000559.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000550.2 Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.

Price & Availability↑

CloneID	OHu25692
Clone ID Related Accession (Same CDS sequence)	NM_000559.2 , NM_000559.3
Accession Version	NM_000559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	hemoglobin subunit gamma-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104389.8. On Dec 4, 2019 this sequence version replaced NM_000559.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCATT TCACAGAGGA GGACAAGGCT ACTATCACAA GCCTGTGGGG CAAGGTGAAT 
GTGGAAGATG CTGGAGGAGA AACCCTGGGA AGGCTCCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTTG ACAGCTTTGG CAACCTGTCC TCTGCCTCTG CCATCATGGG CAACCCCAAA 
GTCAAGGCAC ATGGCAAGAA GGTGCTGACT TCCTTGGGAG ATGCCACAAA GCACCTGGAT 
GATCTCAAGG GCACCTTTGC CCAGCTGAGT GAACTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGAAATGTG CTGGTGACCG TTTTGGCAAT CCATTTCGGC 
AAAGAATTCA CCCCTGAGGT GCAGGCTTCC TGGCAGAAGA TGGTGACTGC AGTGGCCAGT 
GCCCTGTCCT CCAGATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000550.2
CDS	54..497
Translation	MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDATKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTAVASALSSRYH

Target ORF information:

RefSeq Version	NM_000559.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hemoglobin subunit gamma 1 (HBG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000559.3

ORF Insert Sequence:

ATGGGTCATT TCACAGAGGA GGACAAGGCT ACTATCACAA GCCTGTGGGG CAAGGTGAAT 
GTGGAAGATG CTGGAGGAGA AACCCTGGGA AGGCTCCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTTG ACAGCTTTGG CAACCTGTCC TCTGCCTCTG CCATCATGGG CAACCCCAAA 
GTCAAGGCAC ATGGCAAGAA GGTGCTGACT TCCTTGGGAG ATGCCACAAA GCACCTGGAT 
GATCTCAAGG GCACCTTTGC CCAGCTGAGT GAACTGCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAAGCTCCT GGGAAATGTG CTGGTGACCG TTTTGGCAAT CCATTTCGGC 
AAAGAATTCA CCCCTGAGGT GCAGGCTTCC TGGCAGAAGA TGGTGACTGC AGTGGCCAGT 
GCCCTGTCCT CCAGATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An A?-globin G->A gene polymorphism associated with ?
BMC medical genetics18(1)93(2017 08)
Breveglieri G,Bianchi N,Cosenza LC,Gamberini MR,Chiavilli F,Zuccato C,Montagner G,Borgatti M,Lampronti I,Finotti A,Gambari R

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HBG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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