PYCR2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYCR2
Entrez Gene ID	29920
Full Name	pyrroline-5-carboxylate reductase 2
Synonyms	HLD10,P5CR2
General protein information	Preferred Names pyrroline-5-carboxylate reductase 2 Names pyrroline-5-carboxylate reductase 2 P5C reductase 2 pyrroline 5-carboxylate reductase isoform pyrroline-5-carboxylate reductase family member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.12
Summary	This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	616406
Disorder Html:	Leukodystrophy, hypomyelinating, 10, 616420 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_013328.3	NP_037460.2	pyrroline-5-carboxylate reductase 2 isoform 1
NM_001271681.1	NP_001258610.1	pyrroline-5-carboxylate reductase 2 isoform 2
NM_013328.4	NP_037460.2	pyrroline-5-carboxylate reductase 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pycr2	NP_001012208.1
Pan troglodytes (chimpanzee)	PYCR2	XP_514237.2
Canis lupus familiaris (dog)	PYCR2	XP_537234.3
Danio rerio (zebrafish)	pycr1a	NP_957120.1
Xenopus tropicalis (tropical clawed frog)	pycr1	NP_001015942.1
Homo sapiens (human)	PYCR2	NP_037460.2
	PYCR2	XP_001092867.1
Bos taurus (cattle)	PYCR2	NP_001068649.1
Mus musculus (house mouse)	Pycr2	NP_598466.1
Gallus gallus (chicken)	PYCR2	XP_001232432.2
Danio rerio (zebrafish)	pycr1b	NP_001018454.1

Gene Ontology
Bibliography

Related articles in PubMed

PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Zaki MS, Bhat G, Sultan T, Issa M, Jung HJ, Dikoglu E, Selim L, G Mahmoud I, Abdel-Hamid MS, Abdel-Salam G, Marin-Valencia I, Gleeson JG
Annals of neurology80(1)59-70(2016 Jul)

Downregulation of pyrroline-5-carboxylate reductase-2 induces the autophagy of melanoma cells via AMPK/mTOR pathway.
Ou R, Zhang X, Cai J, Shao X, Lv M, Qiu W, Xuan X, Liu J, Li Z, Xu Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(5)6485-91(2016 May)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress.
Kuo ML, Lee MB, Tang M, den Besten W, Hu S, Sweredoski MJ, Hess S, Chou CM, Changou CA, Su M, Jia W, Su L, Yen Y
Scientific reports618846(2016 Jan)

Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.
Nakayama T, Al-Maawali A, El-Quessny M, Rajab A, Khalil S, Stoler JM, Tan WH, Nasir R, Schmitz-Abe K, Hill RS, Partlow JN, Al-Saffar M, Servattalab S, LaCoursiere CM, Tambunan DE, Coulter ME, Elhosary PC, Gorski G, Barkovich AJ, Markianos K, Poduri A, Mochida GH
American journal of human genetics96(5)709-19(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PYCR2-related syndrome represents a clinically recognizable condition in which PYCR2 mutations lead to protein dysfunction.
Title: PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.

It was found that silence of PYCR2 resulted in the decrease of proliferative ability and activation of AMPK/mTOR-induced autophagy of A375 cells. PYCR2 silencing also activated AMPK/mTOR pathway in another melanoma cell line, CHL-1.
Title: Downregulation of pyrroline-5-carboxylate reductase-2 induces the autophagy of melanoma cells via AMPK/mTOR pathway.

Silencing of both PYCR1 and PYCR2 completely abolished anti-oxidation activity of RRM2B, demonstrating a functional collaboration of these metabolic enzymes in response to oxidative stress.
Title: PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress.

Hypomyelination and the absence of lax caused by previously reported mutations in the gene encoding PYCR2's isozyme, PYCR1, suggesting a unique and indispensable role for PYCR2 in the human CNS during development.
Title: Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.

The following PYCR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYCR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06505	NM_013328.4 Latest version!	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu03864	NM_001271681.1 Latest version!	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu06505	NM_013328.3	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYCR2 ( NM_013328.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037460.2 Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06505
Clone ID Related Accession (Same CDS sequence)	NM_013328.3 , NM_013328.4
Accession Version	NM_013328.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	pyrroline-5-carboxylate reductase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY044355.1, BC020553.1 and AW166558.1. This sequence is a reference standard in the RefSeqGene project. On Nov 7, 2012 this sequence version replaced NM_013328.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.37865.1, SRR1163658.307249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGAA GCTGATGGCA TTCCAGCCAG CCCCCAAAGT GATTCGCTGC 
ATGACCAACA CACCTGTGGT AGTGCAGGAA GGCGCTACAG TGTACGCCAC GGGCACCCAT 
GCCCTGGTGG AGGATGGGCA GCTCCTGGAG CAGCTCATGA GCAGCGTGGG CTTCTGCACT 
GAGGTGGAAG AGGACCTCAT CGATGCCGTC ACGGGGCTCA GTGGCAGCGG GCCTGCCTAT 
GCATTCATGG CTCTGGACGC ATTGGCTGAT GGTGGGGTGA AGATGGGTTT GCCACGGCGC 
CTGGCAATCC AACTCGGGGC CCAGGCTTTG CTGGGAGCTG CCAAGATGCT GCTGGACTCG 
GAGCAGCATC CATGCCAGCT TAAGGACAAT GTCTGCTCCC CTGGGGGAGC CACCATCCAC 
GCCCTGCACT TTCTAGAGAG TGGGGGCTTC CGCTCTCTGC TCATCAATGC AGTTGAGGCC 
TCCTGTATCC GAACACGAGA GCTACAGTCC ATGGCCGACC AAGAAAAGAT CTCCCCAGCT 
GCCCTTAAGA AGACCCTCTT AGACAGAGTG AAGCTGGAAT CCCCCACAGT CTCCACACTG 
ACCCCCTCCA GCCCAGGGAA GCTCCTCACA AGAAGCCTGG CCCTGGGAGG CAAGAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037460.2
CDS	231..1193
Translation	MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

Target ORF information:

RefSeq Version	NM_013328.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013328.3

ORF Insert Sequence:

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGAA GCTGATGGCA TTCCAGCCAG CCCCCAAAGT GATTCGCTGC 
ATGACCAACA CACCTGTGGT AGTGCAGGAA GGCGCTACAG TGTACGCCAC GGGCACCCAT 
GCCCTGGTGG AGGATGGGCA GCTCCTGGAG CAGCTCATGA GCAGCGTGGG CTTCTGCACT 
GAGGTGGAAG AGGACCTCAT CGATGCCGTC ACGGGGCTCA GTGGCAGCGG GCCTGCCTAT 
GCATTCATGG CTCTGGACGC ATTGGCTGAT GGTGGGGTGA AGATGGGTTT GCCACGGCGC 
CTGGCAATCC AACTCGGGGC CCAGGCTTTG CTGGGAGCTG CCAAGATGCT GCTGGACTCG 
GAGCAGCATC CATGCCAGCT TAAGGACAAT GTCTGCTCCC CTGGGGGAGC CACCATCCAC 
GCCCTGCACT TTCTAGAGAG TGGGGGCTTC CGCTCTCTGC TCATCAATGC AGTTGAGGCC 
TCCTGTATCC GAACACGAGA GCTACAGTCC ATGGCCGACC AAGAAAAGAT CTCCCCAGCT 
GCCCTTAAGA AGACCCTCTT AGACAGAGTG AAGCTGGAAT CCCCCACAGT CTCCACACTG 
ACCCCCTCCA GCCCAGGGAA GCTCCTCACA AGAAGCCTGG CCCTGGGAGG CAAGAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYCR2 ( NM_013328.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037460.2 Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06505
Clone ID Related Accession (Same CDS sequence)	NM_013328.3 , NM_013328.4
Accession Version	NM_013328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	pyrroline-5-carboxylate reductase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC020553.1 and AW166558.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_013328.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.273299.1, SRR3476690.534714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000343818.11/ ENSP00000342502.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGAA GCTGATGGCA TTCCAGCCAG CCCCCAAAGT GATTCGCTGC 
ATGACCAACA CACCTGTGGT AGTGCAGGAA GGCGCTACAG TGTACGCCAC GGGCACCCAT 
GCCCTGGTGG AGGATGGGCA GCTCCTGGAG CAGCTCATGA GCAGCGTGGG CTTCTGCACT 
GAGGTGGAAG AGGACCTCAT CGATGCCGTC ACGGGGCTCA GTGGCAGCGG GCCTGCCTAT 
GCATTCATGG CTCTGGACGC ATTGGCTGAT GGTGGGGTGA AGATGGGTTT GCCACGGCGC 
CTGGCAATCC AACTCGGGGC CCAGGCTTTG CTGGGAGCTG CCAAGATGCT GCTGGACTCG 
GAGCAGCATC CATGCCAGCT TAAGGACAAT GTCTGCTCCC CTGGGGGAGC CACCATCCAC 
GCCCTGCACT TTCTAGAGAG TGGGGGCTTC CGCTCTCTGC TCATCAATGC AGTTGAGGCC 
TCCTGTATCC GAACACGAGA GCTACAGTCC ATGGCCGACC AAGAAAAGAT CTCCCCAGCT 
GCCCTTAAGA AGACCCTCTT AGACAGAGTG AAGCTGGAAT CCCCCACAGT CTCCACACTG 
ACCCCCTCCA GCCCAGGGAA GCTCCTCACA AGAAGCCTGG CCCTGGGAGG CAAGAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037460.2
CDS	141..1103
Translation	MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

Target ORF information:

RefSeq Version	NM_013328.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013328.4

ORF Insert Sequence:

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGAA GCTGATGGCA TTCCAGCCAG CCCCCAAAGT GATTCGCTGC 
ATGACCAACA CACCTGTGGT AGTGCAGGAA GGCGCTACAG TGTACGCCAC GGGCACCCAT 
GCCCTGGTGG AGGATGGGCA GCTCCTGGAG CAGCTCATGA GCAGCGTGGG CTTCTGCACT 
GAGGTGGAAG AGGACCTCAT CGATGCCGTC ACGGGGCTCA GTGGCAGCGG GCCTGCCTAT 
GCATTCATGG CTCTGGACGC ATTGGCTGAT GGTGGGGTGA AGATGGGTTT GCCACGGCGC 
CTGGCAATCC AACTCGGGGC CCAGGCTTTG CTGGGAGCTG CCAAGATGCT GCTGGACTCG 
GAGCAGCATC CATGCCAGCT TAAGGACAAT GTCTGCTCCC CTGGGGGAGC CACCATCCAC 
GCCCTGCACT TTCTAGAGAG TGGGGGCTTC CGCTCTCTGC TCATCAATGC AGTTGAGGCC 
TCCTGTATCC GAACACGAGA GCTACAGTCC ATGGCCGACC AAGAAAAGAT CTCCCCAGCT 
GCCCTTAAGA AGACCCTCTT AGACAGAGTG AAGCTGGAAT CCCCCACAGT CTCCACACTG 
ACCCCCTCCA GCCCAGGGAA GCTCCTCACA AGAAGCCTGG CCCTGGGAGG CAAGAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYCR2 ( NM_001271681.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258610.1 Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03864
Clone ID Related Accession (Same CDS sequence)	NM_001271681.1
Accession Version	NM_001271681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-06
Organism	Homo sapiens(human)
Product	pyrroline-5-carboxylate reductase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY044355.1, BU538765.1, BC014868.1 and HY264354.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BU538765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGGC ATTCATGGCT CTGGACGCAT TGGCTGATGG TGGGGTGAAG 
ATGGGTTTGC CACGGCGCCT GGCAATCCAA CTCGGGGCCC AGGCTTTGCT GGGAGCTGCC 
AAGATGCTGC TGGACTCGGA GCAGCATCCA TGCCAGCTTA AGGACAATGT CTGCTCCCCT 
GGGGGAGCCA CCATCCACGC CCTGCACTTT CTAGAGAGTG GGGGCTTCCG CTCTCTGCTC 
ATCAATGCAG TTGAGGCCTC CTGTATCCGA ACACGAGAGC TACAGTCCAT GGCCGACCAA 
GAAAAGATCT CCCCAGCTGC CCTTAAGAAG ACCCTCTTAG ACAGAGTGAA GCTGGAATCC 
CCCACAGTCT CCACACTGAC CCCCTCCAGC CCAGGGAAGC TCCTCACAAG AAGCCTGGCC 
CTGGGAGGCA AGAAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258610.1
CDS	231..971
Translation	MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

Target ORF information:

RefSeq Version	NM_001271681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyrroline-5-carboxylate reductase 2 (PYCR2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271681.1

ORF Insert Sequence:

ATGAGCGTGG GCTTCATCGG GGCCGGCCAG CTGGCCTATG CTCTGGCGCG GGGCTTCACG 
GCCGCAGGCA TCCTGTCGGC TCACAAGATA ATAGCCAGCT CCCCAGAAAT GAACCTGCCC 
ACGGTGTCCG CGCTCAGGAA GATGGGTGTG AACCTGACAC GCAGCAACAA GGAGACGGTG 
AAGCACAGCG ACGTCCTGTT TCTGGCTGTG AAGCCACATA TCATCCCCTT CATCCTGGAT 
GAGATTGGGG CCGACGTGCA AGCCAGACAC ATCGTGGTCT CCTGTGCGGC TGGTGTCACC 
ATCAGCTCTG TGGAGAAGGC ATTCATGGCT CTGGACGCAT TGGCTGATGG TGGGGTGAAG 
ATGGGTTTGC CACGGCGCCT GGCAATCCAA CTCGGGGCCC AGGCTTTGCT GGGAGCTGCC 
AAGATGCTGC TGGACTCGGA GCAGCATCCA TGCCAGCTTA AGGACAATGT CTGCTCCCCT 
GGGGGAGCCA CCATCCACGC CCTGCACTTT CTAGAGAGTG GGGGCTTCCG CTCTCTGCTC 
ATCAATGCAG TTGAGGCCTC CTGTATCCGA ACACGAGAGC TACAGTCCAT GGCCGACCAA 
GAAAAGATCT CCCCAGCTGC CCTTAAGAAG ACCCTCTTAG ACAGAGTGAA GCTGGAATCC 
CCCACAGTCT CCACACTGAC CCCCTCCAGC CCAGGGAAGC TCCTCACAAG AAGCCTGGCC 
CTGGGAGGCA AGAAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Annals of neurology80(1)59-70(2016 Jul)
Zaki MS,Bhat G,Sultan T,Issa M,Jung HJ,Dikoglu E,Selim L,G Mahmoud I,Abdel-Hamid MS,Abdel-Salam G,Marin-Valencia I,Gleeson JG

Downregulation of pyrroline-5-carboxylate reductase-2 induces the autophagy of melanoma cells via AMPK/mTOR pathway.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(5)6485-91(2016 May)
Ou R,Zhang X,Cai J,Shao X,Lv M,Qiu W,Xuan X,Liu J,Li Z,Xu Y

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress.
Scientific reports618846(2016 Jan)
Kuo ML,Lee MB,Tang M,den Besten W,Hu S,Sweredoski MJ,Hess S,Chou CM,Changou CA,Su M,Jia W,Su L,Yen Y

Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.
American journal of human genetics96(5)709-19(2015 May)
Nakayama T,Al-Maawali A,El-Quessny M,Rajab A,Khalil S,Stoler JM,Tan WH,Nasir R,Schmitz-Abe K,Hill RS,Partlow JN,Al-Saffar M,Servattalab S,LaCoursiere CM,Tambunan DE,Coulter ME,Elhosary PC,Gorski G,Barkovich AJ,Markianos K,Poduri A,Mochida GH

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PYCR2 cDNA ORF clone, Homo sapiens(human)

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