GUCY1A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY1A2
Entrez Gene ID	2977
Full Name	guanylate cyclase 1 soluble subunit alpha 2
Synonyms	GC-SA2,GUC1A2
General protein information	Preferred Names guanylate cyclase 1 soluble subunit alpha 2 Names guanylate cyclase soluble subunit alpha-2 GCS-alpha-2 guanylate cyclase 1, soluble, alpha 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q22.3
Summary	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	601244

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256424.1	NP_001243353.1	guanylate cyclase soluble subunit alpha-2 isoform 1
NM_000855.3	NP_000846.1	guanylate cyclase soluble subunit alpha-2 isoform 2
NM_001256424.2	NP_001243353.1	guanylate cyclase soluble subunit alpha-2 isoform 1
NM_000855.2	NP_000846.1	guanylate cyclase soluble subunit alpha-2 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GUCY1A2	XP_536590.2
Rattus norvegicus (Norway rat)	Gucy1a2	NP_076446.1
	GUCY1A2	XP_002799832.1
Gallus gallus (chicken)	GUCY1A2	XP_004938891.1
Pan troglodytes (chimpanzee)	GUCY1A2	XP_001136693.1
Bos taurus (cattle)	GUCY1A2	NP_001178270.1
Mus musculus (house mouse)	Gucy1a2	NP_001028494.1
Drosophila melanogaster (fruit fly)	Gycalpha99B	NP_477088.2
Homo sapiens (human)	GUCY1A2	NP_001243353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000280	XP_310840.5
Xenopus tropicalis (tropical clawed frog)	gucy1a2	NP_001120379.1

Gene Ontology
Bibliography

Related articles in PubMed

Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity.
Seeger F, Quintyn R, Tanimoto A, Williams GJ, Tainer JA, Wysocki VH, Garcin ED
Biochemistry53(13)2153-65(2014 Apr)

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes.
Ivarsson Y, Arnold R, McLaughlin M, Nim S, Joshi R, Ray D, Liu B, Teyra J, Pawson T, Moffat J, Li SS, Sidhu SS, Kim PM
Proceedings of the National Academy of Sciences of the United States of America111(7)2542-7(2014 Feb)

Genome-wide and gene-based association studies of anxiety disorders in European and African American samples.
Otowa T, Maher BS, Aggen SH, McClay JL, van den Oord EJ, Hettema JM
PloS one9(11)e112559(2014)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data support a novel regulatory mechanism whereby sGC activity is tuned by distinct domain interactions that either promote or inhibit catalytic activity.
Title: Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity.

The CO binding affinity of soluble guanylate cyclase alpha 2 subunit is threefold greater than that of human soluble guanylate cyclase alpha 1 subunit.
Title: Structural and functional insights into the heme-binding domain of the human soluble guanylate cyclase ?2 subunit and heterodimeric ?2?1.

Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
Title: Gene-environment interaction in the etiology of mathematical ability using SNP sets.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The sGC alpha 1observed in OVCAR-3 and MDA-MB-468 cancer cells which correlated well with the sGC activity and a marked increase in cGMP levels upon exposure to the combination of a NO donor and a sGC activator.
Title: Role of soluble guanylyl cyclase-cyclic GMP signaling in tumor cell proliferation.

The following GUCY1A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY1A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10941	NM_001256424.2 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu10685	NM_000855.3 Latest version!	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
View Old Accession Versions >>
OHu10941	NM_001256424.1	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu10685	NM_000855.2	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY1A2 ( NM_001256424.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243353.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10941
Clone ID Related Accession (Same CDS sequence)	NM_001256424.1 , NM_001256424.2
Accession Version	NM_001256424.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z50053.1, AP005014.3 and CA774108.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z50053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGCCCC AGAGGAGTGA ACTCCTTTTC 
TCCTTTCCTG TTTCCATCCA GCTGGTTCCA GATCAGCATC AAAGTGAAAC GGATTTGGGT 
ACTGAAAAGA TGAGGATAGG AATTCACTCA GGCTCCGTGC TGGCTGGAGT TGTTGGGGTG 
CGAATGCCAC GTTATTGCCT GTTTGGAAAT AATGTCACAC TGGCAAGCAA ATTCGAGTCG 
GGAAGTCACC CTCGGCGCAT CAATGTCAGC CCAACCACTT ACCAATTATT AAAACGAGAA 
GAAAGTTTCA CATTCATTCC GCGGTCTCGT GAAGAGCTTC CAGACAACTT TCCAAAGGAA 
ATTCCTGGGA TCTGCTATTT CCTGGAGGTA AGGACTGGTC CAAAGCCACC AAAGCCTTCT 
CTTTCTTCGT CGAGAATAAA AAAGGTTTCC TACAACATCG GCACCATGTT CCTCCGGGAG 
ACAAGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243353.1
CDS	391..2682
Translation	MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPGPLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSPQTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTANILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQATLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKLCSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFDPSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKPEASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQPQRSELLFSFPVSIQLVPDQHQSETDLGTEKMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL

Target ORF information:

RefSeq Version	NM_001256424.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256424.1

ORF Insert Sequence:

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGCCCC AGAGGAGTGA ACTCCTTTTC 
TCCTTTCCTG TTTCCATCCA GCTGGTTCCA GATCAGCATC AAAGTGAAAC GGATTTGGGT 
ACTGAAAAGA TGAGGATAGG AATTCACTCA GGCTCCGTGC TGGCTGGAGT TGTTGGGGTG 
CGAATGCCAC GTTATTGCCT GTTTGGAAAT AATGTCACAC TGGCAAGCAA ATTCGAGTCG 
GGAAGTCACC CTCGGCGCAT CAATGTCAGC CCAACCACTT ACCAATTATT AAAACGAGAA 
GAAAGTTTCA CATTCATTCC GCGGTCTCGT GAAGAGCTTC CAGACAACTT TCCAAAGGAA 
ATTCCTGGGA TCTGCTATTT CCTGGAGGTA AGGACTGGTC CAAAGCCACC AAAGCCTTCT 
CTTTCTTCGT CGAGAATAAA AAAGGTTTCC TACAACATCG GCACCATGTT CCTCCGGGAG 
ACAAGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A2 ( NM_001256424.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243353.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10941
Clone ID Related Accession (Same CDS sequence)	NM_001256424.1 , NM_001256424.2
Accession Version	NM_001256424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003078.2, Z50053.1, AP005014.3 and CA774108.1. On Jun 2, 2019 this sequence version replaced NM_001256424.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z50053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGCCCC AGAGGAGTGA ACTCCTTTTC 
TCCTTTCCTG TTTCCATCCA GCTGGTTCCA GATCAGCATC AAAGTGAAAC GGATTTGGGT 
ACTGAAAAGA TGAGGATAGG AATTCACTCA GGCTCCGTGC TGGCTGGAGT TGTTGGGGTG 
CGAATGCCAC GTTATTGCCT GTTTGGAAAT AATGTCACAC TGGCAAGCAA ATTCGAGTCG 
GGAAGTCACC CTCGGCGCAT CAATGTCAGC CCAACCACTT ACCAATTATT AAAACGAGAA 
GAAAGTTTCA CATTCATTCC GCGGTCTCGT GAAGAGCTTC CAGACAACTT TCCAAAGGAA 
ATTCCTGGGA TCTGCTATTT CCTGGAGGTA AGGACTGGTC CAAAGCCACC AAAGCCTTCT 
CTTTCTTCGT CGAGAATAAA AAAGGTTTCC TACAACATCG GCACCATGTT CCTCCGGGAG 
ACAAGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243353.1
CDS	422..2713
Translation	MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPGPLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSPQTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTANILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQATLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKLCSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFDPSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKPEASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQPQRSELLFSFPVSIQLVPDQHQSETDLGTEKMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL

Target ORF information:

RefSeq Version	NM_001256424.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256424.2

ORF Insert Sequence:

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGCCCC AGAGGAGTGA ACTCCTTTTC 
TCCTTTCCTG TTTCCATCCA GCTGGTTCCA GATCAGCATC AAAGTGAAAC GGATTTGGGT 
ACTGAAAAGA TGAGGATAGG AATTCACTCA GGCTCCGTGC TGGCTGGAGT TGTTGGGGTG 
CGAATGCCAC GTTATTGCCT GTTTGGAAAT AATGTCACAC TGGCAAGCAA ATTCGAGTCG 
GGAAGTCACC CTCGGCGCAT CAATGTCAGC CCAACCACTT ACCAATTATT AAAACGAGAA 
GAAAGTTTCA CATTCATTCC GCGGTCTCGT GAAGAGCTTC CAGACAACTT TCCAAAGGAA 
ATTCCTGGGA TCTGCTATTT CCTGGAGGTA AGGACTGGTC CAAAGCCACC AAAGCCTTCT 
CTTTCTTCGT CGAGAATAAA AAAGGTTTCC TACAACATCG GCACCATGTT CCTCCGGGAG 
ACAAGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A2 ( NM_000855.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000846.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10685
Clone ID Related Accession (Same CDS sequence)	NM_000855.3 , NM_000855.2
Accession Version	NM_000855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003078.2, X63282.1, AP005014.3 and CA774108.1. On Jun 2, 2019 this sequence version replaced NM_000855.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138518.269321.1, X63282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGATGA GGATAGGAAT TCACTCAGGC 
TCCGTGCTGG CTGGAGTTGT TGGGGTGCGA ATGCCACGTT ATTGCCTGTT TGGAAATAAT 
GTCACACTGG CAAGCAAATT CGAGTCGGGA AGTCACCCTC GGCGCATCAA TGTCAGCCCA 
ACCACTTACC AATTATTAAA ACGAGAAGAA AGTTTCACAT TCATTCCGCG GTCTCGTGAA 
GAGCTTCCAG ACAACTTTCC AAAGGAAATT CCTGGGATCT GCTATTTCCT GGAGGTAAGG 
ACTGGTCCAA AGCCACCAAA GCCTTCTCTT TCTTCGTCGA GAATAAAAAA GGTTTCCTAC 
AACATCGGCA CCATGTTCCT CCGGGAGACA AGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000846.1
CDS	422..2620
Translation	MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPGPLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSPQTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTANILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQATLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKLCSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFDPSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKPEASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL

Target ORF information:

RefSeq Version	NM_000855.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000855.3

ORF Insert Sequence:

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGATGA GGATAGGAAT TCACTCAGGC 
TCCGTGCTGG CTGGAGTTGT TGGGGTGCGA ATGCCACGTT ATTGCCTGTT TGGAAATAAT 
GTCACACTGG CAAGCAAATT CGAGTCGGGA AGTCACCCTC GGCGCATCAA TGTCAGCCCA 
ACCACTTACC AATTATTAAA ACGAGAAGAA AGTTTCACAT TCATTCCGCG GTCTCGTGAA 
GAGCTTCCAG ACAACTTTCC AAAGGAAATT CCTGGGATCT GCTATTTCCT GGAGGTAAGG 
ACTGGTCCAA AGCCACCAAA GCCTTCTCTT TCTTCGTCGA GAATAAAAAA GGTTTCCTAC 
AACATCGGCA CCATGTTCCT CCGGGAGACA AGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A2 ( NM_000855.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000846.1 Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10685
Clone ID Related Accession (Same CDS sequence)	NM_000855.3 , NM_000855.2
Accession Version	NM_000855.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Homo sapiens(human)
Product	guanylate cyclase soluble subunit alpha-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X63282.1, AP005014.3 and CA774108.1. On Jan 27, 2012 this sequence version replaced NM_000855.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63282.1, BC130484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGATGA GGATAGGAAT TCACTCAGGC 
TCCGTGCTGG CTGGAGTTGT TGGGGTGCGA ATGCCACGTT ATTGCCTGTT TGGAAATAAT 
GTCACACTGG CAAGCAAATT CGAGTCGGGA AGTCACCCTC GGCGCATCAA TGTCAGCCCA 
ACCACTTACC AATTATTAAA ACGAGAAGAA AGTTTCACAT TCATTCCGCG GTCTCGTGAA 
GAGCTTCCAG ACAACTTTCC AAAGGAAATT CCTGGGATCT GCTATTTCCT GGAGGTAAGG 
ACTGGTCCAA AGCCACCAAA GCCTTCTCTT TCTTCGTCGA GAATAAAAAA GGTTTCCTAC 
AACATCGGCA CCATGTTCCT CCGGGAGACA AGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000846.1
CDS	391..2589
Translation	MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPGPLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSPQTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTANILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQATLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKLCSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFDPSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKPEASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL

Target ORF information:

RefSeq Version	NM_000855.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000855.2

ORF Insert Sequence:

ATGTCTCGAA GGAAGATTTC GTCCGAGTCC TTCAGCTCCC TGGGCTCCGA CTACCTGGAG 
ACCAGCCCGG AGGAGGAGGG GGAGTGCCCC CTGTCTAGGC TCTGCTGGAA TGGCAGCCGG 
AGCCCGCCCG GGCCGCTGGA GCCCAGCCCG GCCGCAGCTG CCGCTGCCGC CGCCCCGGCC 
CCGACCCCGG CTGCTTCTGC CGCCGCCGCC GCTGCCACTG CCGGGGCCAG GAGGGTGCAG 
CGCCGGAGGC GGGTCAACCT GGACTCGCTG GGCGAGAGCA TCAGCCGCCT GACGGCGCCC 
TCGCCTCAGA CGATACAGCA GACTCTCAAG AGGACACTGC AGTATTATGA ACATCAAGTT 
ATTGGTTACA GGGATGCAGA AAAGAATTTC CACAATATCT CTAACAGATG CTCCTATGCA 
GACCACTCCA ACAAAGAAGA AATTGAAGAT GTCTCAGGAA TTCTTCAGTG TACTGCTAAT 
ATACTCGGTT TGAAGTTTGA GGAAATTCAA AAAAGATTTG GTGAAGAGTT CTTTAATATA 
TGCTTTCATG AGAATGAGAG AGTCCTTCGA GCTGTAGGTG GCACTTTGCA GGACTTTTTT 
AACGGCTTTG ATGCTTTGTT GGAACACATT AGAACTTCTT TTGGAAAACA GGCCACTCTG 
GAGTCACCAT CTTTCCTATG CAAAGAGCTC CCTGAAGGTA CTCTCATGCT CCACTACTTC 
CACCCTCACC ATATTGTGGG GTTTGCAATG CTGGGGATGA TTAAGGCTGC AGGAAAGAAG 
ATCTATCGGC TGGATGTGGA AGTGGAACAG GTTGCAAATG AGAAGCTATG CTCTGATGTT 
TCAAACCCAG GCAATTGTAG CTGTCTTACT TTCCTTATCA AAGAATGTGA AAATACTAAT 
ATCATGAAGA ACCTTCCACA GGGAACCTCC CAAGTTCCTG CGGACCTCAG AATTAGCATC 
AACACCTTCT GTAGAGCCTT CCCTTTCCAC TTGATGTTTG ATCCCAGCAT GTCAGTCCTT 
CAGTTGGGGG AAGGTCTAAG GAAGCAGCTT CGATGTGACA CTCACAAAGT GCTCAAGTTT 
GAGGACTGCT TCGAGATTGT ATCTCCAAAG GTTAATGCCA CCTTTGAAAG GGTCCTGCTG 
CGACTGTCTA CCCCGTTTGT GATTAGAACC AAGCCTGAGG CTTCTGGCTC TGAAAATAAA 
GACAAGGTGA TGGAAGTCAA AGGACAAATG ATCCATGTTC CAGAATCAAA TTCCATTTTA 
TTTTTGGGCT CTCCATGTGT GGACAAGTTG GATGAACTCA TGGGCCGAGG GCTACATCTC 
TCAGACATCC CTATCCATGA TGCCACCCGA GATGTCATTT TGGTTGGTGA GCAGGCAAAG 
GCCCAAGATG GGTTGAAGAA AAGGATGGAT AAATTAAAGG CAACTTTAGA AAGAACTCAC 
CAGGCCCTGG AAGAAGAGAA AAAGAAGACA GTGGATCTTC TATATTCTAT TTTCCCTGGT 
GATGTAGCCC AGCAATTATG GCAAGGGCAG CAAGTACAGG CCAGAAAGTT TGATGATGTC 
ACCATGCTCT TTTCAGACAT TGTTGGCTTC ACAGCCATAT GTGCCCAGTG TACTCCCATG 
CAAGTAATCA GCATGCTGAA TGAACTGTAC ACCAGATTTG ACCACCAGTG TGGATTTTTG 
GATATTTATA AGGTGGAAAC AATAGGTGAT GCCTACTGTG TTGCAGCAGG GCTCCACAGA 
AAAAGCCTCT GCCATGCTAA ACCCATTGCT CTGATGGCCT TGAAGATGAT GGAACTTTCA 
GAAGAGGTGC TGACACCTGA TGGAAGACCG ATTCAGATGA GGATAGGAAT TCACTCAGGC 
TCCGTGCTGG CTGGAGTTGT TGGGGTGCGA ATGCCACGTT ATTGCCTGTT TGGAAATAAT 
GTCACACTGG CAAGCAAATT CGAGTCGGGA AGTCACCCTC GGCGCATCAA TGTCAGCCCA 
ACCACTTACC AATTATTAAA ACGAGAAGAA AGTTTCACAT TCATTCCGCG GTCTCGTGAA 
GAGCTTCCAG ACAACTTTCC AAAGGAAATT CCTGGGATCT GCTATTTCCT GGAGGTAAGG 
ACTGGTCCAA AGCCACCAAA GCCTTCTCTT TCTTCGTCGA GAATAAAAAA GGTTTCCTAC 
AACATCGGCA CCATGTTCCT CCGGGAGACA AGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity.
Biochemistry53(13)2153-65(2014 Apr)
Seeger F,Quintyn R,Tanimoto A,Williams GJ,Tainer JA,Wysocki VH,Garcin ED

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes.
Proceedings of the National Academy of Sciences of the United States of America111(7)2542-7(2014 Feb)
Ivarsson Y,Arnold R,McLaughlin M,Nim S,Joshi R,Ray D,Liu B,Teyra J,Pawson T,Moffat J,Li SS,Sidhu SS,Kim PM

Genome-wide and gene-based association studies of anxiety disorders in European and African American samples.
PloS one9(11)e112559(2014)
Otowa T,Maher BS,Aggen SH,McClay JL,van den Oord EJ,Hettema JM

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

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GUCY1A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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