GSPT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GSPT1
Entrez Gene ID	2935
Full Name	G1 to S phase transition 1
Synonyms	551G9.2,ETF3A,GST1,eRF3a
General protein information	Preferred Names G1 to S phase transition 1 Names eukaryotic peptide chain release factor GTP-binding subunit ERF3A G1 to S phase transition protein 1 homolog eukaryotic peptide chain release factor subunit 3a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.13
Disorder MIM:	139259

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130006.2	NP_001123478.2	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
NM_001130007.2	NP_001123479.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3
NM_001130006.1	NP_001123478.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
NM_002094.4	NP_002085.3	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
NM_002094.3	NP_002085.2	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
NM_001130007.1	NP_001123479.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GSPT1	XP_001140542.3
Rattus norvegicus (Norway rat)	Gspt1	NP_001003978.1
Gallus gallus (chicken)	GSPT1	NP_001129149.1
Saccharomyces cerevisiae (baker's yeast)	SUP35	NP_010457.3
Homo sapiens (human)	GSPT1	NP_001123478.1
Danio rerio (zebrafish)	gspt1	NP_001003992.1
Kluyveromyces lactis	KLLA0D17424g	XP_453831.1
Oryza sativa (rice)	Os04g0270100	NP_001052341.1
	LOC716860	XP_001107851.2
Bos taurus (cattle)	GSPT1	NP_001091540.2
Mus musculus (house mouse)	Gspt1	NP_001123480.1
Rattus norvegicus (Norway rat)	LOC100911685	XP_006245802.1
Eremothecium gossypii	AGOS_AGL145W	NP_986522.2
Danio rerio (zebrafish)	gspt1l	NP_942101.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009310	XP_320105.3
Schizosaccharomyces pombe (fission yeast)	sup35	NP_588225.1
Neurospora crassa	NCU04790	XP_960416.1
Arabidopsis thaliana (thale cress)	AT1G18070	NP_001185026.1
Canis lupus familiaris (dog)	GSPT1	XP_005621589.1
Drosophila melanogaster (fruit fly)	Elf	NP_001260415.1
Magnaporthe oryzae (rice blast fungus)	MGG_00449	XP_003718618.1
Xenopus tropicalis (tropical clawed frog)	gspt1	NP_001015805.2

Gene Ontology
Bibliography

Related articles in PubMed

PABP enhances release factor recruitment and stop codon recognition during translation termination.
Ivanov A, Mikhailova T, Eliseev B, Yeramala L, Sokolova E, Susorov D, Shuvalov A, Schaffitzel C, Alkalaeva E
Nucleic acids research44(16)7766-76(2016 Sep)

Studies on human eRF3-PABP interaction reveal the influence of eRF3a N-terminal glycin repeat on eRF3-PABP binding affinity and the lower affinity of eRF3a 12-GGC allele involved in cancer susceptibility.
Jerbi S, Jolles B, Bouceba T, Jean-Jean O
RNA biology13(3)306-15(2016)

Identification of four new susceptibility loci for testicular germ cell tumour.
Litchfield K, Holroyd A, Lloyd A, Broderick P, Nsengimana J, Eeles R, Easton DF, Dudakia D, Bishop DT, Reid A, Huddart RA, Grotmol T, Wiklund F, Shipley J, Houlston RS, Turnbull C
Nature communications68690(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PABP enhances the productive binding of the eRF1-eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination.
Title: PABP enhances release factor recruitment and stop codon recognition during translation termination.

Data found that the N-terminal glycine repeat of eRF3a influences eRF3a-PABP interaction and that eRF3a 12-GGC allele has a decreased binding affinity for PABP.
Title: Studies on human eRF3-PABP interaction reveal the influence of eRF3a N-terminal glycin repeat on eRF3-PABP binding affinity and the lower affinity of eRF3a 12-GGC allele involved in cancer susceptibility.

GAB2, GSPT1, TFDP2 and ZFPM1 are four new susceptibility loci for testicular germ cell tumor.
Title: Identification of four new susceptibility loci for testicular germ cell tumour.

Overexpression of ID1 in two different cell lines induced STMN3 and GSPT1 at the transcriptional level, while depletion of ID1 reduced their expression.
Title: Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner.

The proteolytic cleavage of eRF3a and eRF3b into p-eRF3 leads to release an amino-terminal fragment containing nuclear export signal to allow the relocalization of eRF3 into the nucleus to interact with the p14ARF.
Title: The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor.

The following GSPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24371	NM_001130007.2 Latest version!	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24372	NM_002094.3 Latest version!	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107280	NM_002094.4 Latest version!	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu108240	NM_001130006.2 Latest version!	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu23466	NM_001130006.1 Latest version!	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu24371	NM_001130007.1	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GSPT1 ( NM_001130007.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123479.1 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24371
Clone ID Related Accession (Same CDS sequence)	NM_001130007.2 , NM_001130007.1
Accession Version	NM_001130007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2, AK315267.1, BT006722.1 and BM666324.1. On May 31, 2019 this sequence version replaced NM_001130007.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminal, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.203571.1, SRR1660805.140418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA 
AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT 
AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT 
GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG 
GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT 
TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA 
GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT 
GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG 
CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA 
AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT 
AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA 
CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC 
TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT 
GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA 
CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG 
CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC 
GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA 
AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT 
TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA 
GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT 
GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT 
AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA 
GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA 
GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123479.1
CDS	676..2175
Translation	MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001130007.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130007.2

ORF Insert Sequence:

ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA 
AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT 
AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT 
GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG 
GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT 
TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA 
GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT 
GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG 
CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA 
AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT 
AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA 
CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC 
TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT 
GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA 
CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG 
CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC 
GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA 
AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT 
TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA 
GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT 
GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT 
AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA 
GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA 
GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT1 ( NM_001130007.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123479.1 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24371
Clone ID Related Accession (Same CDS sequence)	NM_001130007.2 , NM_001130007.1
Accession Version	NM_001130007.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2, AK315267.1, BT006722.1 and BM666324.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminal, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.203571.1, SRR1660805.140418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA 
AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT 
AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT 
GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG 
GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT 
TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA 
GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT 
GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG 
CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA 
AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT 
AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA 
CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC 
TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT 
GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA 
CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG 
CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC 
GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA 
AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT 
TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA 
GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT 
GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT 
AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA 
GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA 
GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123479.1
CDS	684..2183
Translation	MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001130007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130007.1

ORF Insert Sequence:

ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA 
AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT 
AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT 
GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG 
GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT 
TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA 
GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT 
GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG 
CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA 
AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT 
AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA 
CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC 
TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT 
GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA 
CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG 
CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC 
GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA 
AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT 
TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA 
GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT 
GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT 
AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA 
GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA 
GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT1 ( NM_002094.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002085.2 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24372
Clone ID Related Accession (Same CDS sequence)	NM_002094.3
Accession Version	NM_002094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132091.1, DC384910.1, BC008391.1, BT006722.1, AC007216.2 and BM666324.1. On Jul 10, 2008 this sequence version replaced NM_002094.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.43615.1, SRR1660805.27369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG 
CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG 
CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT 
GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC 
GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC 
AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC 
TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG 
GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC 
AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA 
ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA 
CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG 
GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG 
CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA 
GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA 
CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC 
AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA 
TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA 
GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC 
AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT 
CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC 
AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG 
GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC 
CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC 
ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG 
GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002085.2
CDS	249..2162
Translation	MDPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPAGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_002094.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002094.3

ORF Insert Sequence:

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG 
CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG 
CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT 
GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC 
GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC 
AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC 
TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG 
GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC 
AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA 
ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA 
CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG 
GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG 
CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA 
GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA 
CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC 
AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA 
TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA 
GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC 
AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT 
CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC 
AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG 
GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC 
CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC 
ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG 
GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT1 ( NM_002094.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002085.3 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

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CloneID	OHu107280
Clone ID Related Accession (Same CDS sequence)	NM_002094.4
Accession Version	NM_002094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2. On Nov 23, 2018 this sequence version replaced NM_002094.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.43615.1, SRR1660805.27369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000434724.7/ ENSP00000398131.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG 
CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG 
CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT 
GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC 
GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC 
AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC 
TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG 
GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC 
AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA 
ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA 
CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG 
GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG 
CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA 
GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA 
CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC 
AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA 
TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA 
GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC 
AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT 
CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC 
AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG 
GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC 
CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC 
ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG 
GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002085.3
CDS	237..2150
Translation	MDPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPVGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_002094.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002094.4

ORF Insert Sequence:

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG 
CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG 
CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT 
GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC 
GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC 
AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC 
TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG 
GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC 
AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA 
ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA 
CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG 
GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG 
CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA 
GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA 
CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC 
AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA 
TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA 
GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC 
AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT 
CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC 
AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG 
GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC 
CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC 
ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG 
GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT1 ( NM_001130006.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123478.2 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

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CloneID	OHu108240
Clone ID Related Accession (Same CDS sequence)	NM_001130006.2
Accession Version	NM_001130006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2. On May 31, 2019 this sequence version replaced NM_001130006.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009503.2, SRR1803611.160983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA 
GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG 
GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA 
AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT 
CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT 
GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA 
CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG 
TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA 
GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA 
TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA 
CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC 
TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123478.2
CDS	237..2147
Translation	MDPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPVGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001130006.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130006.2

ORF Insert Sequence:

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA 
GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG 
GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA 
AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT 
CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT 
GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA 
CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG 
TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA 
GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA 
TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA 
CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC 
TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT1 ( NM_001130006.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123478.1 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

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CloneID	OHu23466
Clone ID Related Accession (Same CDS sequence)	NM_001130006.1
Accession Version	NM_001130006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132091.1, DC384910.1, BC008391.1, BC009503.2, AC007216.2 and BM666324.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.310946.1, SRR1163658.413921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA 
GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG 
GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA 
AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT 
CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT 
GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA 
CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG 
TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA 
GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA 
TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA 
CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC 
TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123478.1
CDS	249..2159
Translation	MDPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPAGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001130006.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130006.1

ORF Insert Sequence:

ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG 
CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC 
CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC 
CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT 
GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG 
GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA 
CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA 
GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG 
GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA 
AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT 
CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT 
GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA 
CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG 
TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA 
GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA 
TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA 
CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC 
TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PABP enhances release factor recruitment and stop codon recognition during translation termination.
Nucleic acids research44(16)7766-76(2016 Sep)
Ivanov A,Mikhailova T,Eliseev B,Yeramala L,Sokolova E,Susorov D,Shuvalov A,Schaffitzel C,Alkalaeva E

Studies on human eRF3-PABP interaction reveal the influence of eRF3a N-terminal glycin repeat on eRF3-PABP binding affinity and the lower affinity of eRF3a 12-GGC allele involved in cancer susceptibility.
RNA biology13(3)306-15(2016)
Jerbi S,Jolles B,Bouceba T,Jean-Jean O

Identification of four new susceptibility loci for testicular germ cell tumour.
Nature communications68690(2015 Oct)
Litchfield K,Holroyd A,Lloyd A,Broderick P,Nsengimana J,Eeles R,Easton DF,Dudakia D,Bishop DT,Reid A,Huddart RA,Grotmol T,Wiklund F,Shipley J,Houlston RS,Turnbull C

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GSPT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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