GSPT1 cDNA ORF clone, Homo sapiens(human)

The following GSPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24371 NM_001130007.2
Latest version!
Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24372 NM_002094.3
Latest version!
Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu107280 NM_002094.4
Latest version!
Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu108240 NM_001130006.2
Latest version!
Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu23466 NM_001130006.1
Latest version!
Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00
View Old Accession Versions >>
OHu24371 NM_001130007.1 Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24371
    Clone ID Related Accession (Same CDS sequence) NM_001130007.2 , NM_001130007.1
    Accession Version NM_001130007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2, AK315267.1, BT006722.1 and BM666324.1. On May 31, 2019 this sequence version replaced NM_001130007.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminal, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.203571.1, SRR1660805.140418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
    TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA
    AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT
    AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT
    GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG
    GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT
    TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA
    GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT
    GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG
    CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA
    AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT
    AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA
    CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC
    TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT
    GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA
    CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG
    CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC
    GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA
    AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT
    TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA
    GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT
    GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT
    AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA
    GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA
    GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123479.1
    CDS676..2175
    Translation

    Target ORF information:

    RefSeq Version NM_001130007.2
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
    TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA
    AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT
    AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT
    GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG
    GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT
    TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA
    GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT
    GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG
    CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA
    AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT
    AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA
    CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC
    TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT
    GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA
    CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG
    CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC
    GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA
    AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT
    TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA
    GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT
    GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT
    AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA
    GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA
    GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24371
    Clone ID Related Accession (Same CDS sequence) NM_001130007.2 , NM_001130007.1
    Accession Version NM_001130007.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2, AK315267.1, BT006722.1 and BM666324.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminal, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.203571.1, SRR1660805.140418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
    TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA
    AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT
    AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT
    GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG
    GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT
    TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA
    GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT
    GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG
    CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA
    AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT
    AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA
    CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC
    TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT
    GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA
    CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG
    CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC
    GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA
    AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT
    TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA
    GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT
    GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT
    AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA
    GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA
    GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123479.1
    CDS684..2183
    Translation

    Target ORF information:

    RefSeq Version NM_001130007.1
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAACTTT CAGAACCTAT TGTAGAAAAT GGAGAGACAG AAATGTCTCC AGAAGAATCA 
    TGGGAGCACA AAGAAGAAAT AAGTGAAGCA GAGCCAGGGG GTGGTTCCTT GGGAGATGGA
    AGGCCGCCAG AGGAAAGTGC CCATGAAATG ATGGAGGAGG AAGAGGAAAT CCCAAAACCT
    AAGTCTGTGG TTGCACCGCC AGGTGCTCCT AAGAAAGAGC ATGTAAATGT AGTATTCATT
    GGGCACGTAG ATGCTGGCAA GTCAACCATT GGAGGACAAA TAATGTATTT GACTGGAATG
    GTTGACAAAA GGACGCTTGA AAAGTATGAA AGAGAAGCTA AAGAGAAAAA CAGAGAAACT
    TGGTACTTGT CTTGGGCCTT AGACACAAAT CAGGAAGAAC GAGACAAGGG TAAAACAGTA
    GAAGTGGGTC GTGCCTATTT TGAAACCGAA AAGAAGCATT TCACAATTCT AGATGCCCCT
    GGCCACAAGA GTTTTGTCCC AAATATGATT GGTGGTGCCT CTCAAGCTGA TTTGGCTGTG
    CTGGTAATCT CAGCCAGGAA AGGAGAGTTT GAAACTGGAT TTGAAAAAGG AGGACAGACA
    AGAGAACATG CAATGTTGGC AAAGACAGCA GGTGTAAAAC ACCTAATTGT GCTAATTAAT
    AAGATGGATG ATCCAACAGT AAATTGGAGC AATGAGAGAT ATGAAGAATG TAAGGAGAAA
    CTAGTGCCAT TTTTGAAAAA AGTTGGCTTC AATCCCAAAA AGGACATTCA CTTTATGCCC
    TGCTCAGGAC TTACTGGAGC AAATCTCAAA GAGCAGTCGG ATTTCTGTCC TTGGTACATT
    GGATTACCGT TTATTCCATA TCTGGATAAT TTGCCGAACT TCAATAGATC AGTTGATGGA
    CCAATCAGGC TGCCAATTGT GGATAAGTAC AAGGATATGG GCACTGTGGT CCTGGGAAAG
    CTGGAATCAG GATCTATTTG TAAAGGCCAG CAGCTTGTGA TGATGCCAAA CAAGCACAAC
    GTGGAAGTTC TTGGAATACT TTCCGATGAT GTAGAGACTG ATACCGTAGC CCCAGGTGAA
    AACCTCAAAA TCAGACTGAA AGGAATTGAA GAAGAGGAGA TTCTTCCAGG GTTTATACTT
    TGTGATCCTA ATAATCTTTG TCATTCTGGA CGCACATTTG ATGCCCAGAT AGTGATTATA
    GAGCACAAAT CCATCATCTG CCCAGGCTAT AATGCGGTGC TGCATATTCA TACCTGTATT
    GAGGAGGTGG AAATAACAGC CTTAATCTGC TTGGTAGACA AAAAATCAGG AGAAAAAAGT
    AAGACCCGAC CCCGTTTTGT CAAACAAGAT CAAGTATGCA TTGCTCGCTT AAGGACAGCA
    GGAACCATCT GCCTTGAGAC CTTTAAAGAC TTCCCTCAGA TGGGTCGTTT CACCTTAAGA
    GATGAGGGTA AGACCATTGC AATTGGAAAA GTTCTGAAAC TGGTTCCAGA GAAAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24372
    Clone ID Related Accession (Same CDS sequence) NM_002094.3
    Accession Version NM_002094.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132091.1, DC384910.1, BC008391.1, BT006722.1, AC007216.2 and BM666324.1. On Jul 10, 2008 this sequence version replaced NM_002094.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.43615.1, SRR1660805.27369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG
    CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG
    CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT
    GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC
    GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC
    AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC
    TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG
    GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC
    AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA
    ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA
    CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG
    GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG
    CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA
    GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA
    CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC
    AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA
    TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA
    GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC
    AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT
    CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC
    AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG
    GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC
    CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC
    ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG
    GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002085.2
    CDS249..2162
    Translation

    Target ORF information:

    RefSeq Version NM_002094.3
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002094.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG
    CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG
    CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT
    GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC
    GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC
    AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC
    TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG
    GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC
    AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA
    ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA
    CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG
    GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG
    CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA
    GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA
    CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC
    AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA
    TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA
    GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC
    AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT
    CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC
    AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG
    GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC
    CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC
    ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG
    GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107280
    Clone ID Related Accession (Same CDS sequence) NM_002094.4
    Accession Version NM_002094.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2. On Nov 23, 2018 this sequence version replaced NM_002094.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.43615.1, SRR1660805.27369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000434724.7/ ENSP00000398131.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG
    CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG
    CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT
    GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC
    GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC
    AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC
    TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG
    GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC
    AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA
    ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA
    CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG
    GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG
    CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA
    GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA
    CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC
    AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA
    TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA
    GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC
    AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT
    CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC
    AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG
    GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC
    CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC
    ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG
    GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002085.3
    CDS237..2150
    Translation

    Target ORF information:

    RefSeq Version NM_002094.4
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002094.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGTAGA AAATGGAGAG ACAGAAATGT CTCCAGAAGA ATCATGGGAG
    CACAAAGAAG AAATAAGTGA AGCAGAGCCA GGGGGTGGTT CCTTGGGAGA TGGAAGGCCG
    CCAGAGGAAA GTGCCCATGA AATGATGGAG GAGGAAGAGG AAATCCCAAA ACCTAAGTCT
    GTGGTTGCAC CGCCAGGTGC TCCTAAGAAA GAGCATGTAA ATGTAGTATT CATTGGGCAC
    GTAGATGCTG GCAAGTCAAC CATTGGAGGA CAAATAATGT ATTTGACTGG AATGGTTGAC
    AAAAGGACGC TTGAAAAGTA TGAAAGAGAA GCTAAAGAGA AAAACAGAGA AACTTGGTAC
    TTGTCTTGGG CCTTAGACAC AAATCAGGAA GAACGAGACA AGGGTAAAAC AGTAGAAGTG
    GGTCGTGCCT ATTTTGAAAC CGAAAAGAAG CATTTCACAA TTCTAGATGC CCCTGGCCAC
    AAGAGTTTTG TCCCAAATAT GATTGGTGGT GCCTCTCAAG CTGATTTGGC TGTGCTGGTA
    ATCTCAGCCA GGAAAGGAGA GTTTGAAACT GGATTTGAAA AAGGAGGACA GACAAGAGAA
    CATGCAATGT TGGCAAAGAC AGCAGGTGTA AAACACCTAA TTGTGCTAAT TAATAAGATG
    GATGATCCAA CAGTAAATTG GAGCAATGAG AGATATGAAG AATGTAAGGA GAAACTAGTG
    CCATTTTTGA AAAAAGTTGG CTTCAATCCC AAAAAGGACA TTCACTTTAT GCCCTGCTCA
    GGACTTACTG GAGCAAATCT CAAAGAGCAG TCGGATTTCT GTCCTTGGTA CATTGGATTA
    CCGTTTATTC CATATCTGGA TAATTTGCCG AACTTCAATA GATCAGTTGA TGGACCAATC
    AGGCTGCCAA TTGTGGATAA GTACAAGGAT ATGGGCACTG TGGTCCTGGG AAAGCTGGAA
    TCAGGATCTA TTTGTAAAGG CCAGCAGCTT GTGATGATGC CAAACAAGCA CAACGTGGAA
    GTTCTTGGAA TACTTTCCGA TGATGTAGAG ACTGATACCG TAGCCCCAGG TGAAAACCTC
    AAAATCAGAC TGAAAGGAAT TGAAGAAGAG GAGATTCTTC CAGGGTTTAT ACTTTGTGAT
    CCTAATAATC TTTGTCATTC TGGACGCACA TTTGATGCCC AGATAGTGAT TATAGAGCAC
    AAATCCATCA TCTGCCCAGG CTATAATGCG GTGCTGCATA TTCATACCTG TATTGAGGAG
    GTGGAAATAA CAGCCTTAAT CTGCTTGGTA GACAAAAAAT CAGGAGAAAA AAGTAAGACC
    CGACCCCGTT TTGTCAAACA AGATCAAGTA TGCATTGCTC GCTTAAGGAC AGCAGGAACC
    ATCTGCCTTG AGACCTTTAA AGACTTCCCT CAGATGGGTC GTTTCACCTT AAGAGATGAG
    GGTAAGACCA TTGCAATTGG AAAAGTTCTG AAACTGGTTC CAGAGAAAGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108240
    Clone ID Related Accession (Same CDS sequence) NM_001130006.2
    Accession Version NM_001130006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007216.2. On May 31, 2019 this sequence version replaced NM_001130006.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009503.2, SRR1803611.160983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC
    AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA
    GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG
    GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA
    GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA
    AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG
    TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT
    CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG
    AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC
    TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT
    GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT
    GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA
    TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA
    CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG
    TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG
    CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA
    GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT
    CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA
    ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT
    AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA
    TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG
    GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA
    CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC
    TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT
    AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123478.2
    CDS237..2147
    Translation

    Target ORF information:

    RefSeq Version NM_001130006.2
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGTT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC
    AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA
    GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG
    GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA
    GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA
    AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG
    TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT
    CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG
    AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC
    TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT
    GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT
    GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA
    TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA
    CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG
    TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG
    CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA
    GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT
    CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA
    ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT
    AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA
    TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG
    GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA
    CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC
    TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT
    AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23466
    Clone ID Related Accession (Same CDS sequence) NM_001130006.1
    Accession Version NM_001130006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132091.1, DC384910.1, BC008391.1, BC009503.2, AC007216.2 and BM666324.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.310946.1, SRR1163658.413921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC
    AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA
    GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG
    GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA
    GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA
    AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG
    TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT
    CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG
    AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC
    TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT
    GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT
    GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA
    TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA
    CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG
    TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG
    CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA
    GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT
    CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA
    ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT
    AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA
    TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG
    GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA
    CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC
    TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT
    AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123478.1
    CDS249..2159
    Translation

    Target ORF information:

    RefSeq Version NM_001130006.1
    Organism Homo sapiens(human)
    Definition Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATCCGG GCAGTGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGGAG CAGCAGCGGC 
    AGCAGCAGCA GCGACTCGGC GCCTGACTGC TGGGACCAGG CGGACATGGA AGCCCCCGGG
    CCGGGCCCTT GCGGCGGCGG CGGCTCCCTG GCGGCGGCGG CCGAGGCCCA GCGGGAGAAC
    CTCAGCGCGG CCTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCCTTCGT GCCCAACGTC
    CACGCCGCCG AGTTCGTGCC GTCCTTCCTG CGGGGCCCGG CAGCGCCGCC ACCCCCAGCT
    GGCGGCGCCG CCAATAACCA CGGAGCCGGC AGCGGCGCGG GAGGCCGTGC GGCACCTGTG
    GAATCCTCTC AAGAGGAACA GTCATTGTGT GAAGGTTCAA ATTCAGCTGT TAGCATGGAA
    CTTTCAGAAC CTATTGAAAA TGGAGAGACA GAAATGTCTC CAGAAGAATC ATGGGAGCAC
    AAAGAAGAAA TAAGTGAAGC AGAGCCAGGG GGTGGTTCCT TGGGAGATGG AAGGCCGCCA
    GAGGAAAGTG CCCATGAAAT GATGGAGGAG GAAGAGGAAA TCCCAAAACC TAAGTCTGTG
    GTTGCACCGC CAGGTGCTCC TAAGAAAGAG CATGTAAATG TAGTATTCAT TGGGCACGTA
    GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTTGACAAA
    AGGACGCTTG AAAAGTATGA AAGAGAAGCT AAAGAGAAAA ACAGAGAAAC TTGGTACTTG
    TCTTGGGCCT TAGACACAAA TCAGGAAGAA CGAGACAAGG GTAAAACAGT AGAAGTGGGT
    CGTGCCTATT TTGAAACCGA AAAGAAGCAT TTCACAATTC TAGATGCCCC TGGCCACAAG
    AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ATTTGGCTGT GCTGGTAATC
    TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT
    GCAATGTTGG CAAAGACAGC AGGTGTAAAA CACCTAATTG TGCTAATTAA TAAGATGGAT
    GATCCAACAG TAAATTGGAG CAATGAGAGA TATGAAGAAT GTAAGGAGAA ACTAGTGCCA
    TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ACTTTATGCC CTGCTCAGGA
    CTTACTGGAG CAAATCTCAA AGAGCAGTCG GATTTCTGTC CTTGGTACAT TGGATTACCG
    TTTATTCCAT ATCTGGATAA TTTGCCGAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG
    CTGCCAATTG TGGATAAGTA CAAGGATATG GGCACTGTGG TCCTGGGAAA GCTGGAATCA
    GGATCTATTT GTAAAGGCCA GCAGCTTGTG ATGATGCCAA ACAAGCACAA CGTGGAAGTT
    CTTGGAATAC TTTCCGATGA TGTAGAGACT GATACCGTAG CCCCAGGTGA AAACCTCAAA
    ATCAGACTGA AAGGAATTGA AGAAGAGGAG ATTCTTCCAG GGTTTATACT TTGTGATCCT
    AATAATCTTT GTCATTCTGG ACGCACATTT GATGCCCAGA TAGTGATTAT AGAGCACAAA
    TCCATCATCT GCCCAGGCTA TAATGCGGTG CTGCATATTC ATACCTGTAT TGAGGAGGTG
    GAAATAACAG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACCCGA
    CCCCGTTTTG TCAAACAAGA TCAAGTATGC ATTGCTCGCT TAAGGACAGC AGGAACCATC
    TGCCTTGAGA CCTTTAAAGA CTTCCCTCAG ATGGGTCGTT TCACCTTAAG AGATGAGGGT
    AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.