ARHGAP35 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP35
Entrez Gene ID	2909
Full Name	Rho GTPase activating protein 35
Synonyms	GRF-1,GRLF1,P190-A,P190A,p190ARhoGAP,p190RhoGAP
General protein information	Preferred Names Rho GTPase activating protein 35 Names rho GTPase-activating protein 35 glucocorticoid receptor DNA-binding factor 1 glucocorticoid receptor repression factor 1 rho GAP p190A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Summary	The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008].
Disorder MIM:	605277

mRNA and Protein(s)

mRNA	Protein	Name
XM_011526873.2	XP_011525175.1	rho GTPase-activating protein 35 isoform X2
XM_024451473.1	XP_024307241.1	rho GTPase-activating protein 35 isoform X1
XM_017026714.1	XP_016882203.1	rho GTPase-activating protein 35 isoform X1
NM_004491.5	NP_004482.4	rho GTPase-activating protein 35
NM_004491.4	NP_004482.4	rho GTPase-activating protein 35

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Mus musculus (house mouse)	Grlf1	NP_766327.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Gene Ontology
Bibliography

Related articles in PubMed

Low Frequent Mutation of ARHGAP35, a Candidate Tumor Suppressor Gene, in Gastric and Colorectal Cancers.
Choi EJ, Kim MS, Song SY, Yoo NJ, Lee SH
Pathology oncology research : POR24(1)175-176(2018 01)

Gene-based association studies report genetic links for clinical subtypes of frontotemporal dementia.
Mishra A, Ferrari R, Heutink P, Hardy J, Pijnenburg Y, Posthuma D,
Brain : a journal of neurology140(5)1437-1446(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

report association of APOE and TOMM40 with behavioural variant frontotemporal dementia, and ARHGAP35 and SERPINA1 with progressive non-fluent aphasia.
Title: Gene-based association studies report genetic links for clinical subtypes of frontotemporal dementia.

interaction involves the first FF motif of p190A and the winged helix/PCI domain of eIF3A, is enhanced by serum stimulation and reduced by phosphatase treatment
Title: Interaction of p190A RhoGAP with eIF3A and Other Translation Preinitiation Factors Suggests a Role in Protein Biosynthesis.

these data demonstrate that a complex of p190RhoGAP-A and anillin modulates RhoA-GTP levels in the cytokinetic furrow to ensure progression of cytokinesis.
Title: A complex of p190RhoGAP-A and anillin modulates RhoA-GTP and the cytokinetic furrow in human cells.

GRF-1 expression may modify osteosarcoma prognosis and may be a potential tumor therapeutic target.
Title: Glucocorticoid receptor DNA binding factor 1 expression and osteosarcoma prognosis.

ARHGAP35 rs1052667 polymorphism was an independent prognostic factor influencing the survival of osteosarcoma.
Title: Rho GTPase-activating protein 35 rs1052667 polymorphism and osteosarcoma risk and prognosis.

The following ARHGAP35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27217	NM_004491.5 Latest version!	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu27217	XM_017026714.1 Latest version!	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu58112	XM_011526873.2 Latest version!	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu58112	XM_024451473.1 Latest version!	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu27217	NM_004491.4	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP35 ( NM_004491.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004482.4 Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.

Price & Availability↑

CloneID	OHu27217
Clone ID Related Accession (Same CDS sequence)	XM_017026714.1 , NM_004491.5 , NM_004491.4
Accession Version	NM_004491.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008895.7, AB051509.2, BE871213.1, CX163309.1, AC008755.8 and CA429690.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2019 this sequence version replaced NM_004491.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2616084.1, SRR1803612.13661.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000672722.1/ ENSP00000500409.1 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004482.4
CDS	402..4901
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRPITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQAEHTL

Target ORF information:

RefSeq Version	NM_004491.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004491.5

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP35 ( NM_004491.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004482.4 Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.

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CloneID	OHu27217
Clone ID Related Accession (Same CDS sequence)	XM_017026714.1 , NM_004491.5 , NM_004491.4
Accession Version	NM_004491.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB051509.2, BE871213.1, CX163309.1, AC008755.8 and CA429690.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2007 this sequence version replaced NM_004491.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051509.2, BC150257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004482.4
CDS	1..4500
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRPITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQAEHTL

Target ORF information:

RefSeq Version	NM_004491.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004491.4

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP35 ( XM_017026714.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882203.1 Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27217
Clone ID Related Accession (Same CDS sequence)	XM_017026714.1 , NM_004491.5 , NM_004491.4
Accession Version	XM_017026714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882203.1
CDS	397..4896
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRPITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQAEHTL

Target ORF information:

RefSeq Version	XM_017026714.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026714.1

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AAGGTCAGCC ACAACAACAA GGTGAATCTC ATGACCAGCG AGAACCTCTC CATCTGCTTC 
TGGCCCACCT TGATGAGACC TGATTTCAGC ACTATGGACG CCCTCACAGC CACGCGCACC 
TACCAGACAA TCATTGAACT CTTTATCCAG CAGTGCCCCT TCTTCTTCTA CAATCGGCCC 
ATCACCGAGC CCCCCGGCGC CAGGCCCAGC TCCCCCTCTG CCGTGGCTTC CACCGTCCCC 
TTCCTCACTT CCACGCCTGT CACAAGTCAG CCGTCGCCCC CACAGTCGCC TCCACCCACC 
CCCCAGTCCC CAATGCAGCC ACTGCTTCCC TCCCAGCTTC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP35 ( XM_011526873.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525175.1 Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X2, mRNA.

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CloneID	OHu58112
Clone ID Related Accession (Same CDS sequence)	XM_011526873.2 , XM_024451473.1
Accession Version	XM_011526873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AACTGGGGAG AACCGGGACA CTGCACCTCG AGAGATGTGC TGCAGGAGAC AGCCAGCCTC 
CGGCATGGAG CACAGCCGCA GGCGGGAGGC CGTATGGTTG TTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525175.1
CDS	397..4644
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNNWGEPGHCTSRDVLQETASLRHGAQPQAGGRMVVP

Target ORF information:

RefSeq Version	XM_011526873.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526873.2

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AACTGGGGAG AACCGGGACA CTGCACCTCG AGAGATGTGC TGCAGGAGAC AGCCAGCCTC 
CGGCATGGAG CACAGCCGCA GGCGGGAGGC CGTATGGTTG TTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP35 ( XM_024451473.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307241.1 Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.

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CloneID	OHu58112
Clone ID Related Accession (Same CDS sequence)	XM_011526873.2 , XM_024451473.1
Accession Version	XM_024451473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AACTGGGGAG AACCGGGACA CTGCACCTCG AGAGATGTGC TGCAGGAGAC AGCCAGCCTC 
CGGCATGGAG CACAGCCGCA GGCGGGAGGC CGTATGGTTG TTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307241.1
CDS	110..4357
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNNWGEPGHCTSRDVLQETASLRHGAQPQAGGRMVVP

Target ORF information:

RefSeq Version	XM_024451473.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451473.1

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATTCCCACCT ACAACATCAG TGTGGTGGGA 
TTATCTGGGA CCGAGAAGGA AAAGGGCCAG TGTGGGATTG GAAAGTCTTG TTTGTGCAAC 
CGCTTCGTGC GCCCGAGTGC TGACGAGTTT CACTTGGACC ATACCTCCGT CCTCAGCACC 
AGTGACTTTG GAGGGCGAGT GGTCAATAAT GACCACTTTC TCTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAATTTATT 
GATGATCAGA CTTTTCAACC TCATCGAAGC ACGGCCCTGC AGCCCTATAT CAAGAGAGCT 
GCTGCGACCA AGCTTGCATC AGCTGAAAAA CTCATGTACT TTTGCACTGA CCAGCTGGGG 
CTGGAGCAGG ACTTTGAGCA GAAACAAATG CCAGACGGAA AGCTGCTGGT TGATGGTTTT 
CTTCTTGGTA TTGATGTTAG CAGGGGCATG AATAGGAACT TTGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT GGTCCTGACT 
AAGTGTGACG AAGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTGGA GACCTCAGCG AGATCCAATG TAAACGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAAAGT CGGGGAAAGA CAAAAATCAT TCCTTATTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AAGACAAGTA TGAGTGGCTG 
GTGAGTCGCA TTGTGAAAAA CCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCTCCAG AATACCAGGA CTATGTCTAC CTGGAAGGGA CTCAGAAAGC CAAGAAGCTG 
TTTCTACAGC ACATCCACCG CCTCAAGCAT GAGCATATCG AGCGTAGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTAGC TTTTGAAGCT CTTATACCTA ATCTAGATGA AATAGACCAC 
CTAAGCTGCA TAAAAGCCAA AAAGCTCTTA GAAACCAAGC CAGAATTCTT GAAGTGGTTT 
GTTGTGCTTG AAGAGACCCC ATGGGATGCC ACCAGTCACA TTGACAACAT GGAAAACGAA 
CGGATTCCCT TTGATTTAAT GGATACCGTC CCTGCAGAGC AGCTATACGA GGCCCACTTA 
GAGAAGCTGA GGAACGAAAG GAAAAGAGTT GAGATGCGAA GGGCGTTTAA AGAAAACCTG 
GAGACTTCTC CTTTCATAAC TCCCGGAAAG CCTTGGGAAG AGGCCCGTAG TTTTATTATG 
AATGAGGATT TCTACCAGTG GCTGGAGGAA TCTGTATACA TGGATATTTA TGGCAAACAC 
CAAAAGCAAA TTATAGATAA AGCAAAGGAA GAATTTCAGG AGTTGCTTTT GGAATATTCA 
GAATTGTTTT ATGAACTGGA GCTGGATGCT AAGCCCAGCA AGGAGAAGAT GGGTGTTATT 
CAGGATGTTC TGGGAGAGGA ACAGCGATTT AAAGCATTAC AAAAGCTCCA AGCAGAGCGT 
GATGCCCTTA TTCTGAAACA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCC 
AGCTGCCCAG CTTGTGTGGA CGCTAAGATT GAGCACTTGA TTAGTTCTCG GTTTATCCGG 
CCGTCTGACC GGAATCAGAA AAATTCACTC TCTGACCCTA ACATTGATAG AATCAACTTG 
GTTATATTGG GCAAAGACGG CCTTGCCCGA GAGTTGGCCA ATGAGATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT GATAGATGGT AAAATGTATG AGCTTTCCCT GAGGCCAATA 
GAGGGGAATG TCAGGCTTCC TGTGAACTCT TTCCAGACGC CAACATTTCA GCCCCACGGC 
TGTCTCTGCC TTTACAATTC AAAGGAATCG CTATCCTATG TAGTGGAAAG TATAGAGAAG 
AGTAGAGAGT CCACGCTGGG CCGGCGGGAT AATCATTTAG TCCATCTCCC CCTTACATTA 
ATTTTGGTTA ACAAGAGAGG AGACACCAGT GGAGAGACTC TGCATAGCTT AATACAGCAA 
GGTCAACAAA TTGCTAGCAA ACTTCAGTGT GTCTTTCTCG ACCCTGCTTC TGCTGGCATT 
GGTTACGGAC GCAACATTAA TGAAAAGCAA ATCAGTCAAG TTTTGAAGGG ACTCCTGGAC 
TCTAAGCGTA ACTTAAACCT GGTCAGTTCT ACTGCTAGCA TCAAAGATTT GGCTGATGTT 
GATCTGCGAA TTGTTATGTG TCTGATGTGT GGAGATCCTT TTAGTGCAGA TGACATACTT 
TTTCCTGTCC TTCAGTCCCA AACCTGTAAA TCTTCCCATT GTGGAAGCAA CAACTCTGTT 
TTACTTGAAC TACCAATCGG ACTGCACAAG AAGCGGATTG AACTGTCTGT TCTTTCATAC 
CATTCCTCCT TTAGCATCAG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCTTT GGCTATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCTATTC AGCTTGTAGC ACTCACTGAT GGCGCTGTAG ATGTCCTGGA CAATGACTTA 
AGTAGGGAAC AGCTAACTGA GGGGGAGGAG ATTGCTCAAG AAATTGACGG AAGGTTCACA 
AGCATCCCCT GTAGCCAACC CCAGCATAAA CTTGAGATCT TTCACCCATT TTTTAAAGAT 
GTGGTGGAAA AAAAGAACAT AATCGAGGCT ACTCATATGT ACGATAATGC TGCCGAGGCC 
TGTAGCACCA CCGAAGAGGT GTTTAACTCC CCCCGGGCAG GATCACCGCT CTGCAACTCA 
AACCTGCAGG ATTCAGAAGA AGATATCGAG CCATCTTACA GCCTGTTTCG AGAAGACACA 
TCACTGCCTT CTCTGTCCAA AGACCATTCT AAGCTCTCTA TGGAACTGGA GGGAAATGAT 
GGGCTGTCTT TCATTATGAG CAATTTTGAG AGTAAACTGA ACAACAAAGT ACCTCCGCCA 
GTCAAACCAA AGCCTCCTGT CCATTTTGAA ATTACAAAGG GGGATCTATC TTATTTAGAC 
CAAGGCCATA GGGATGGACA GAGGAAGTCT GTGTCTTCTA GCCCCTGGCT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA TGCTGAACCC ATGGATGCTG TGGTGAAGCC AAGGAATGAA 
GAAGAAAACA TATACTCCGT GCCCCATGAC AGCACCCAAG GCAAAATCAT CACCATTCGG 
AATATCAACA AAGCCCAGTC CAACGGCAGC GGGAATGGTT CTGACAGTGA AATGGACACC 
AGCTCTCTAG AGCGAGGGCG CAAGGTTTCC ATCGTGAGCA AGCCAGTGCT GTACAGGACG 
AGATGCACCC GGCTGGGGCG GTTTGCTAGT TACCGGACCA GCTTCAGCGT GGGGAGTGAT 
GATGAGCTGG GGCCCATCCG GAAGAAAGAG GAGGATCAGG CATCCCAGGG TTATAAAGGG 
GACAATGCTG TCATTCCATA CGAAACAGAC GAAGACCCGC GGAGGAGGAA TATTCTTCGC 
AGCCTAAGGA GGAACACTAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACTG TCGTGACTCC AGAGAAGCCG 
ATCCCCATTT TTATTGAAAG ATGTATTGAG TACATTGAAG CCACAGGACT GAGCACGGAA 
GGCATCTACC GGGTCAGCGG GAACAAGTCT GAGATGGAGA GTCTGCAGAG ACAGTTTGAT 
CAAGACCACA ACCTGGACCT GGCAGAGAAA GACTTTACGG TGAATACCGT GGCTGGTGCC 
ATGAAGAGCT TTTTCTCAGA ACTGCCTGAC CCCCTGGTCC CGTATAACAT GCAGATCGAC 
TTGGTGGAAG CACACAAAAT CAACGACCGG GAGCAGAAGT TGCATGCCCT TAAGGAGGTA 
TTAAAGAAAT TTCCAAAGGA AAACCACGAA GTCTTCAAGT ATGTCATCTC TCACCTAAAC 
AACTGGGGAG AACCGGGACA CTGCACCTCG AGAGATGTGC TGCAGGAGAC AGCCAGCCTC 
CGGCATGGAG CACAGCCGCA GGCGGGAGGC CGTATGGTTG TTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Low Frequent Mutation of ARHGAP35, a Candidate Tumor Suppressor Gene, in Gastric and Colorectal Cancers.
Pathology oncology research : POR24(1)175-176(2018 01)
Choi EJ,Kim MS,Song SY,Yoo NJ,Lee SH

Gene-based association studies report genetic links for clinical subtypes of frontotemporal dementia.
Brain : a journal of neurology140(5)1437-1446(2017 May)
Mishra A,Ferrari R,Heutink P,Hardy J,Pijnenburg Y,Posthuma D,

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ARHGAP35 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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