GIT1 cDNA ORF clone, Homo sapiens(human)

The following GIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16468 NM_001085454.2
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$389.50-$545.30
$779.00
OHu24250 NM_014030.4
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$384.50-$538.30
$769.00
OHu67278 XM_011524684.2
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu67279 XM_011524685.2
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu104392 XM_024450731.1
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu104393 XM_024450732.1
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu104394 XM_024450733.1
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu104395 XM_024450734.1
Latest version!
Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu16468 NM_001085454.1 Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$389.50-$545.30
$779.00
OHu67279 XM_011524685.1 Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu24250 NM_014030.3 Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$384.50-$538.30
$769.00
OHu67278 XM_011524684.1 Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16468
    Clone ID Related Accession (Same CDS sequence) NM_001085454.2 , NM_001085454.1
    Accession Version NM_001085454.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104564.11, CF994106.1, AB209537.1 and BC006227.2. On Jun 3, 2019 this sequence version replaced NM_001085454.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.164997.1, SRR1803617.95462.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGTG AGTGCCGGAA GACAGTGCCC CCAGAGCCCG GCGCCCCAGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG
    GTCACCATCA CCACCCGAGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001078923.1
    CDS271..2583
    Translation

    Target ORF information:

    RefSeq Version NM_001085454.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001085454.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGTG AGTGCCGGAA GACAGTGCCC CCAGAGCCCG GCGCCCCAGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG
    GTCACCATCA CCACCCGAGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16468
    Clone ID Related Accession (Same CDS sequence) NM_001085454.2 , NM_001085454.1
    Accession Version NM_001085454.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF994106.1, AB209537.1, BC006227.2 and BM984210.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.164997.1, SRR1803617.95462.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGTG AGTGCCGGAA GACAGTGCCC CCAGAGCCCG GCGCCCCAGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG
    GTCACCATCA CCACCCGAGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001078923.1
    CDS215..2527
    Translation

    Target ORF information:

    RefSeq Version NM_001085454.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001085454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGTG AGTGCCGGAA GACAGTGCCC CCAGAGCCCG GCGCCCCAGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG
    GTCACCATCA CCACCCGAGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24250
    Clone ID Related Accession (Same CDS sequence) NM_014030.4 , NM_014030.3
    Accession Version NM_014030.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104564.11, CF994106.1, AF124490.1, DR001908.1, AK024826.1 and BM984210.1. On Jun 2, 2019 this sequence version replaced NM_014030.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124490.1, SRR7346977.1702777.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGAGGCCAG CCCTGGAGCC AGTGCGGAGC
    TCACTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GTGAGTGCCG GAAGACAGTG
    CCCCCAGAGC CCGGCGCCCC AGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC TGCCAAGCAG CTGGTCACCA TCACCACCCG AGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_054749.2
    CDS271..2556
    Translation

    Target ORF information:

    RefSeq Version NM_014030.4
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014030.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGAGGCCAG CCCTGGAGCC AGTGCGGAGC
    TCACTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GTGAGTGCCG GAAGACAGTG
    CCCCCAGAGC CCGGCGCCCC AGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC TGCCAAGCAG CTGGTCACCA TCACCACCCG AGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24250
    Clone ID Related Accession (Same CDS sequence) NM_014030.4 , NM_014030.3
    Accession Version NM_014030.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF994106.1, AF124490.1, DR001908.1, AK024826.1 and BM984210.1. On May 4, 2007 this sequence version replaced NM_014030.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGAGGCCAG CCCTGGAGCC AGTGCGGAGC
    TCACTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GTGAGTGCCG GAAGACAGTG
    CCCCCAGAGC CCGGCGCCCC AGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC TGCCAAGCAG CTGGTCACCA TCACCACCCG AGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_054749.2
    CDS215..2500
    Translation

    Target ORF information:

    RefSeq Version NM_014030.3
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014030.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGAGGCCAG CCCTGGAGCC AGTGCGGAGC
    TCACTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GTGAGTGCCG GAAGACAGTG
    CCCCCAGAGC CCGGCGCCCC AGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC TGCCAAGCAG CTGGTCACCA TCACCACCCG AGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67278
    Clone ID Related Accession (Same CDS sequence) XM_011524684.1 , XM_011524684.2
    Accession Version XM_011524684.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    CCTCACTCCT GCCCCCTCCA GAGGCCAGCC CTGGAGCCAG TGCGGAGCTC ACTGCGGCTG
    CTCAACGCCA GCGCCTACCG GCTGCAGAGT GAGTGCCGGA AGACAGTGCC CCCAGAGCCC
    GGCGCCCCAG TGGACTTCCA GCTGCTGACT CAGCAGGTGA TCCAGTGCGC CTATGACATC
    GCCAAGGCTG CCAAGCAGCT GGTCACCATC ACCACCCGAG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522986.1
    CDS56..2389
    Translation

    Target ORF information:

    RefSeq Version XM_011524684.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524684.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    CCTCACTCCT GCCCCCTCCA GAGGCCAGCC CTGGAGCCAG TGCGGAGCTC ACTGCGGCTG
    CTCAACGCCA GCGCCTACCG GCTGCAGAGT GAGTGCCGGA AGACAGTGCC CCCAGAGCCC
    GGCGCCCCAG TGGACTTCCA GCTGCTGACT CAGCAGGTGA TCCAGTGCGC CTATGACATC
    GCCAAGGCTG CCAAGCAGCT GGTCACCATC ACCACCCGAG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67278
    Clone ID Related Accession (Same CDS sequence) XM_011524684.1 , XM_011524684.2
    Accession Version XM_011524684.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011524684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    CCTCACTCCT GCCCCCTCCA GAGGCCAGCC CTGGAGCCAG TGCGGAGCTC ACTGCGGCTG
    CTCAACGCCA GCGCCTACCG GCTGCAGAGT GAGTGCCGGA AGACAGTGCC CCCAGAGCCC
    GGCGCCCCAG TGGACTTCCA GCTGCTGACT CAGCAGGTGA TCCAGTGCGC CTATGACATC
    GCCAAGGCTG CCAAGCAGCT GGTCACCATC ACCACCCGAG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522986.1
    CDS5..2338
    Translation

    Target ORF information:

    RefSeq Version XM_011524684.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524684.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TTGACTTATC CGAATTGGCC AAAGCTGCTA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTCGCCATG GACGTGTATG ACGAGGTGGA TCGAAGAGAA
    AATGATGCAG TGTGGCTGGC TACCCAAAAC CACAGCACTC TGGTGACAGA GCGCAGTGCC
    GTGCCCTTCC TGCCTGTTAA CCCGGAATAC TCAGCCACGC GGAATCAGGG GCGACAAAAG
    CTGGCCCGCT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATTCT CAGTGAGGCC
    AAGCGGAGAC AGCAGGGCAA GAGCCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTCTG
    CGGAGCCAGA GTGACCTCGA CGACCAACAC GACTACGACA GCGTGGCCTC TGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGCACCGGC GCCACTCGGA GCAACCGGGC CCGGAGCATG
    GACTCCTCGG ACTTGTCTGA CGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG
    GCCCTGGCTA CATCGGAGGC AAAGGTGCAG CAGCTCATGA AGGTCAACAG TAGCCTGAGC
    GACGAGCTCC GGAGGCTGCA GCGAGAGATC CACAAGCTGC AGGCGGAGAA CCTGCAGCTC
    CGGCAGCCTC CAGGGCCGGT GCCCACACCT CCACTCCCCA GTGAACGGGC GGAACACACA
    CCCATGGCGC CAGGCGGGAG CACACACCGC AGGGATCGCC AGGCCTTTTC CATGTATGAA
    CCTGGCTCTG CCCTGAAGCC CTTTGGGGGC CCCCCTGGGG ACGAGCTCAC TACGCGGCTG
    CAGCCTTTCC ACAGCACTGA GCTAGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCT
    GGCCTTTACC GGATCCGGAA AGGGGTGTCT GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCGCTGCTGT CCTGCTCCCA GGAGGGAAGC CGCCACACGA GCAAGCTTTC CCGCCACGGC
    AGTGGAGCCG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCACTGCT GGGGCTGGAA
    GGGAAGAGGT TTCTAGAGCT GGGCAAAGAG GAAGACTTCC ACCCAGAGCT GGAAAGCCTG
    GATGGAGACC TAGATCCTGG GCTTCCCAGC ACAGAGGATG TCATCTTGAA GACAGAGCAG
    GTCACCAAGA ACATTCAGGA ACTGTTGCGG GCAGCCCAGG AGTTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCT GTGACCGAGA TGGCCTCCCT CTTCCCAAAG
    CCTCACTCCT GCCCCCTCCA GAGGCCAGCC CTGGAGCCAG TGCGGAGCTC ACTGCGGCTG
    CTCAACGCCA GCGCCTACCG GCTGCAGAGT GAGTGCCGGA AGACAGTGCC CCCAGAGCCC
    GGCGCCCCAG TGGACTTCCA GCTGCTGACT CAGCAGGTGA TCCAGTGCGC CTATGACATC
    GCCAAGGCTG CCAAGCAGCT GGTCACCATC ACCACCCGAG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67279
    Clone ID Related Accession (Same CDS sequence) XM_011524685.2 , XM_011524685.1
    Accession Version XM_011524685.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011524685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGCCTCACT CCTGCCCCCT CCAGAGGCCA
    GCCCTGGAGC CAGTGCGGAG CTCACTGCGG CTGCTCAACG CCAGCGCCTA CCGGCTGCAG
    AGTGAGTGCC GGAAGACAGT GCCCCCAGAG CCCGGCGCCC CAGTGGACTT CCAGCTGCTG
    ACTCAGCAGG TGATCCAGTG CGCCTATGAC ATCGCCAAGG CTGCCAAGCA GCTGGTCACC
    ATCACCACCC GAGAGAAGAA GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522987.1
    CDS5..2311
    Translation

    Target ORF information:

    RefSeq Version XM_011524685.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524685.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGCCTCACT CCTGCCCCCT CCAGAGGCCA
    GCCCTGGAGC CAGTGCGGAG CTCACTGCGG CTGCTCAACG CCAGCGCCTA CCGGCTGCAG
    AGTGAGTGCC GGAAGACAGT GCCCCCAGAG CCCGGCGCCC CAGTGGACTT CCAGCTGCTG
    ACTCAGCAGG TGATCCAGTG CGCCTATGAC ATCGCCAAGG CTGCCAAGCA GCTGGTCACC
    ATCACCACCC GAGAGAAGAA GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67279
    Clone ID Related Accession (Same CDS sequence) XM_011524685.2 , XM_011524685.1
    Accession Version XM_011524685.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGCCTCACT CCTGCCCCCT CCAGAGGCCA
    GCCCTGGAGC CAGTGCGGAG CTCACTGCGG CTGCTCAACG CCAGCGCCTA CCGGCTGCAG
    AGTGAGTGCC GGAAGACAGT GCCCCCAGAG CCCGGCGCCC CAGTGGACTT CCAGCTGCTG
    ACTCAGCAGG TGATCCAGTG CGCCTATGAC ATCGCCAAGG CTGCCAAGCA GCTGGTCACC
    ATCACCACCC GAGAGAAGAA GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522987.1
    CDS56..2362
    Translation

    Target ORF information:

    RefSeq Version XM_011524685.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524685.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGAA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC GGACCCTGGC 
    TGGGCATCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGC
    CTGGGACGCC ACATCTCCAT TGTCAAGCAC CTTCGCCACA GCGCCTGGCC TCCCACGCTG
    CTGCAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CACAAGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CAGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGCTT
    CCCTGCCGGG ACGATGATGG AGTCACCGCC AAAGACCTCA GCAAGCAACT ACACTCGAGC
    GTGCGGACAG GCAACCTGGA GACATGTCTG CGCCTGCTCT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CAGAGAAGGG CACCACACCT CTGCACGTGG CTGCCAAGGC AGGACAGACA
    CTGCAGGCCG AGCTGCTTGT AGTGTATGGG GCTGACCCTG GCTCCCCTGA TGTTAATGGC
    CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AGCTGGCGGA AAGGCTGGTT
    GAGTGCCAAT ATGAGCTCAC TGACCGGCTG GCCTTCTACC TCTGTGGACG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCTGACA GCCTTGACTT ATCCGAATTG
    GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA
    AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA
    TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT
    GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG
    AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA
    CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC
    GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT
    GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG
    CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG
    ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA
    CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC
    CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG
    GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG
    TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA
    AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA
    GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC
    AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG
    CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGCCTCACT CCTGCCCCCT CCAGAGGCCA
    GCCCTGGAGC CAGTGCGGAG CTCACTGCGG CTGCTCAACG CCAGCGCCTA CCGGCTGCAG
    AGTGAGTGCC GGAAGACAGT GCCCCCAGAG CCCGGCGCCC CAGTGGACTT CCAGCTGCTG
    ACTCAGCAGG TGATCCAGTG CGCCTATGAC ATCGCCAAGG CTGCCAAGCA GCTGGTCACC
    ATCACCACCC GAGAGAAGAA GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104392
    Clone ID Related Accession (Same CDS sequence) XM_024450731.1
    Accession Version XM_024450731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGATC TCGGCAAAAG TGCATGTCTC AGAGCCTTGA CTTATCCGAA
    TTGGCCAAAG CTGCTAAGAA GAAGCTGCAG GCGCTCAGCA ACCGGCTTTT TGAGGAACTC
    GCCATGGACG TGTATGACGA GGTGGATCGA AGAGAAAATG ATGCAGTGTG GCTGGCTACC
    CAAAACCACA GCACTCTGGT GACAGAGCGC AGTGCCGTGC CCTTCCTGCC TGTTAACCCG
    GAATACTCAG CCACGCGGAA TCAGGGGCGA CAAAAGCTGG CCCGCTTTAA TGCCCGAGAG
    TTTGCCACCT TGATCATCGA CATTCTCAGT GAGGCCAAGC GGAGACAGCA GGGCAAGAGC
    CTGAGCAGCC CCACAGACAA CCTCGAGCTG TCTCTGCGGA GCCAGAGTGA CCTCGACGAC
    CAACACGACT ACGACAGCGT GGCCTCTGAC GAGGACACAG ACCAGGAGCC CCTGCGCAGC
    ACCGGCGCCA CTCGGAGCAA CCGGGCCCGG AGCATGGACT CCTCGGACTT GTCTGACGGG
    GCTGTGACGC TGCAGGAGTA CCTGGAGCTG AAGAAGGCCC TGGCTACATC GGAGGCAAAG
    GTGCAGCAGC TCATGAAGGT CAACAGTAGC CTGAGCGACG AGCTCCGGAG GCTGCAGCGA
    GAGATCCACA AGCTGCAGGC GGAGAACCTG CAGCTCCGGC AGCCTCCAGG GCCGGTGCCC
    ACACCTCCAC TCCCCAGTGA ACGGGCGGAA CACACACCCA TGGCGCCAGG CGGGAGCACA
    CACCGCAGGG ATCGCCAGGC CTTTTCCATG TATGAACCTG GCTCTGCCCT GAAGCCCTTT
    GGGGGCCCCC CTGGGGACGA GCTCACTACG CGGCTGCAGC CTTTCCACAG CACTGAGCTA
    GAGGACGACG CCATCTATTC AGTGCACGTC CCTGCTGGCC TTTACCGGAT CCGGAAAGGG
    GTGTCTGCCT CAGCTGTGCC CTTCACTCCC TCCTCCCCGC TGCTGTCCTG CTCCCAGGAG
    GGAAGCCGCC ACACGAGCAA GCTTTCCCGC CACGGCAGTG GAGCCGACAG TGACTATGAG
    AACACGCAAA GTGGGGACCC ACTGCTGGGG CTGGAAGGGA AGAGGTTTCT AGAGCTGGGC
    AAAGAGGAAG ACTTCCACCC AGAGCTGGAA AGCCTGGATG GAGACCTAGA TCCTGGGCTT
    CCCAGCACAG AGGATGTCAT CTTGAAGACA GAGCAGGTCA CCAAGAACAT TCAGGAACTG
    TTGCGGGCAG CCCAGGAGTT CAAGCATGAC AGCTTCGTGC CCTGCTCAGA GAAGATCCAT
    TTGGCTGTGA CCGAGATGGC CTCCCTCTTC CCAAAGCCTC ACTCCTGCCC CCTCCAGAGG
    CCAGCCCTGG AGCCAGTGCG GAGCTCACTG CGGCTGCTCA ACGCCAGCGC CTACCGGCTG
    CAGAGTGAGT GCCGGAAGAC AGTGCCCCCA GAGCCCGGCG CCCCAGTGGA CTTCCAGCTG
    CTGACTCAGC AGGTGATCCA GTGCGCCTAT GACATCGCCA AGGCTGCCAA GCAGCTGGTC
    ACCATCACCA CCCGAGAGAA GAAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306499.1
    CDS16..2445
    Translation

    Target ORF information:

    RefSeq Version XM_024450731.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGATC TCGGCAAAAG TGCATGTCTC AGAGCCTTGA CTTATCCGAA
    TTGGCCAAAG CTGCTAAGAA GAAGCTGCAG GCGCTCAGCA ACCGGCTTTT TGAGGAACTC
    GCCATGGACG TGTATGACGA GGTGGATCGA AGAGAAAATG ATGCAGTGTG GCTGGCTACC
    CAAAACCACA GCACTCTGGT GACAGAGCGC AGTGCCGTGC CCTTCCTGCC TGTTAACCCG
    GAATACTCAG CCACGCGGAA TCAGGGGCGA CAAAAGCTGG CCCGCTTTAA TGCCCGAGAG
    TTTGCCACCT TGATCATCGA CATTCTCAGT GAGGCCAAGC GGAGACAGCA GGGCAAGAGC
    CTGAGCAGCC CCACAGACAA CCTCGAGCTG TCTCTGCGGA GCCAGAGTGA CCTCGACGAC
    CAACACGACT ACGACAGCGT GGCCTCTGAC GAGGACACAG ACCAGGAGCC CCTGCGCAGC
    ACCGGCGCCA CTCGGAGCAA CCGGGCCCGG AGCATGGACT CCTCGGACTT GTCTGACGGG
    GCTGTGACGC TGCAGGAGTA CCTGGAGCTG AAGAAGGCCC TGGCTACATC GGAGGCAAAG
    GTGCAGCAGC TCATGAAGGT CAACAGTAGC CTGAGCGACG AGCTCCGGAG GCTGCAGCGA
    GAGATCCACA AGCTGCAGGC GGAGAACCTG CAGCTCCGGC AGCCTCCAGG GCCGGTGCCC
    ACACCTCCAC TCCCCAGTGA ACGGGCGGAA CACACACCCA TGGCGCCAGG CGGGAGCACA
    CACCGCAGGG ATCGCCAGGC CTTTTCCATG TATGAACCTG GCTCTGCCCT GAAGCCCTTT
    GGGGGCCCCC CTGGGGACGA GCTCACTACG CGGCTGCAGC CTTTCCACAG CACTGAGCTA
    GAGGACGACG CCATCTATTC AGTGCACGTC CCTGCTGGCC TTTACCGGAT CCGGAAAGGG
    GTGTCTGCCT CAGCTGTGCC CTTCACTCCC TCCTCCCCGC TGCTGTCCTG CTCCCAGGAG
    GGAAGCCGCC ACACGAGCAA GCTTTCCCGC CACGGCAGTG GAGCCGACAG TGACTATGAG
    AACACGCAAA GTGGGGACCC ACTGCTGGGG CTGGAAGGGA AGAGGTTTCT AGAGCTGGGC
    AAAGAGGAAG ACTTCCACCC AGAGCTGGAA AGCCTGGATG GAGACCTAGA TCCTGGGCTT
    CCCAGCACAG AGGATGTCAT CTTGAAGACA GAGCAGGTCA CCAAGAACAT TCAGGAACTG
    TTGCGGGCAG CCCAGGAGTT CAAGCATGAC AGCTTCGTGC CCTGCTCAGA GAAGATCCAT
    TTGGCTGTGA CCGAGATGGC CTCCCTCTTC CCAAAGCCTC ACTCCTGCCC CCTCCAGAGG
    CCAGCCCTGG AGCCAGTGCG GAGCTCACTG CGGCTGCTCA ACGCCAGCGC CTACCGGCTG
    CAGAGTGAGT GCCGGAAGAC AGTGCCCCCA GAGCCCGGCG CCCCAGTGGA CTTCCAGCTG
    CTGACTCAGC AGGTGATCCA GTGCGCCTAT GACATCGCCA AGGCTGCCAA GCAGCTGGTC
    ACCATCACCA CCCGAGAGAA GAAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104393
    Clone ID Related Accession (Same CDS sequence) XM_024450732.1
    Accession Version XM_024450732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGATC TCGGCAAAAG TGCATGTCTC AGAGCCTTGA CTTATCCGAA
    TTGGCCAAAG CTGCTAAGAA GAAGCTGCAG GCGCTCAGCA ACCGGCTTTT TGAGGAACTC
    GCCATGGACG TGTATGACGA GGTGGATCGA AGAGAAAATG ATGCAGTGTG GCTGGCTACC
    CAAAACCACA GCACTCTGGT GACAGAGCGC AGTGCCGTGC CCTTCCTGCC TGTTAACCCG
    GAATACTCAG CCACGCGGAA TCAGGGGCGA CAAAAGCTGG CCCGCTTTAA TGCCCGAGAG
    TTTGCCACCT TGATCATCGA CATTCTCAGT GAGGCCAAGC GGAGACAGCA GGGCAAGAGC
    CTGAGCAGCC CCACAGACAA CCTCGAGCTG TCTCTGCGGA GCCAGAGTGA CCTCGACGAC
    CAACACGACT ACGACAGCGT GGCCTCTGAC GAGGACACAG ACCAGGAGCC CCTGCGCAGC
    ACCGGCGCCA CTCGGAGCAA CCGGGCCCGG AGCATGGACT CCTCGGACTT GTCTGACGGG
    GCTGTGACGC TGCAGGAGTA CCTGGAGCTG AAGAAGGCCC TGGCTACATC GGAGGCAAAG
    GTGCAGCAGC TCATGAAGGT CAACAGTAGC CTGAGCGACG AGCTCCGGAG GCTGCAGCGA
    GAGATCCACA AGCTGCAGGC GGAGAACCTG CAGCTCCGGC AGCCTCCAGG GCCGGTGCCC
    ACACCTCCAC TCCCCAGTGA ACGGGCGGAA CACACACCCA TGGCGCCAGG CGGGAGCACA
    CACCGCAGGG ATCGCCAGGC CTTTTCCATG TATGAACCTG GCTCTGCCCT GAAGCCCTTT
    GGGGGCCCCC CTGGGGACGA GCTCACTACG CGGCTGCAGC CTTTCCACAG CACTGAGCTA
    GAGGACGACG CCATCTATTC AGTGCACGTC CCTGCTGGCC TTTACCGGAT CCGGAAAGGG
    GTGTCTGCCT CAGCTGTGCC CTTCACTCCC TCCTCCCCGC TGCTGTCCTG CTCCCAGGAG
    GGAAGCCGCC ACACGAGCAA GCTTTCCCGC CACGGCAGTG GAGCCGACAG TGACTATGAG
    AACACGCAAA GTGGGGACCC ACTGCTGGGG CTGGAAGGGA AGAGGTTTCT AGAGCTGGGC
    AAAGAGGAAG ACTTCCACCC AGAGCTGGAA AGCCTGGATG GAGACCTAGA TCCTGGGCTT
    CCCAGCACAG AGGATGTCAT CTTGAAGACA GAGCAGGTCA CCAAGAACAT TCAGGAACTG
    TTGCGGGCAG CCCAGGAGTT CAAGCATGAC AGCTTCGTGC CCTGCTCAGA GAAGATCCAT
    TTGGCTGTGA CCGAGATGGC CTCCCTCTTC CCAAAGAGGC CAGCCCTGGA GCCAGTGCGG
    AGCTCACTGC GGCTGCTCAA CGCCAGCGCC TACCGGCTGC AGAGTGAGTG CCGGAAGACA
    GTGCCCCCAG AGCCCGGCGC CCCAGTGGAC TTCCAGCTGC TGACTCAGCA GGTGATCCAG
    TGCGCCTATG ACATCGCCAA GGCTGCCAAG CAGCTGGTCA CCATCACCAC CCGAGAGAAG
    AAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306500.1
    CDS16..2424
    Translation

    Target ORF information:

    RefSeq Version XM_024450732.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGATC TCGGCAAAAG TGCATGTCTC AGAGCCTTGA CTTATCCGAA
    TTGGCCAAAG CTGCTAAGAA GAAGCTGCAG GCGCTCAGCA ACCGGCTTTT TGAGGAACTC
    GCCATGGACG TGTATGACGA GGTGGATCGA AGAGAAAATG ATGCAGTGTG GCTGGCTACC
    CAAAACCACA GCACTCTGGT GACAGAGCGC AGTGCCGTGC CCTTCCTGCC TGTTAACCCG
    GAATACTCAG CCACGCGGAA TCAGGGGCGA CAAAAGCTGG CCCGCTTTAA TGCCCGAGAG
    TTTGCCACCT TGATCATCGA CATTCTCAGT GAGGCCAAGC GGAGACAGCA GGGCAAGAGC
    CTGAGCAGCC CCACAGACAA CCTCGAGCTG TCTCTGCGGA GCCAGAGTGA CCTCGACGAC
    CAACACGACT ACGACAGCGT GGCCTCTGAC GAGGACACAG ACCAGGAGCC CCTGCGCAGC
    ACCGGCGCCA CTCGGAGCAA CCGGGCCCGG AGCATGGACT CCTCGGACTT GTCTGACGGG
    GCTGTGACGC TGCAGGAGTA CCTGGAGCTG AAGAAGGCCC TGGCTACATC GGAGGCAAAG
    GTGCAGCAGC TCATGAAGGT CAACAGTAGC CTGAGCGACG AGCTCCGGAG GCTGCAGCGA
    GAGATCCACA AGCTGCAGGC GGAGAACCTG CAGCTCCGGC AGCCTCCAGG GCCGGTGCCC
    ACACCTCCAC TCCCCAGTGA ACGGGCGGAA CACACACCCA TGGCGCCAGG CGGGAGCACA
    CACCGCAGGG ATCGCCAGGC CTTTTCCATG TATGAACCTG GCTCTGCCCT GAAGCCCTTT
    GGGGGCCCCC CTGGGGACGA GCTCACTACG CGGCTGCAGC CTTTCCACAG CACTGAGCTA
    GAGGACGACG CCATCTATTC AGTGCACGTC CCTGCTGGCC TTTACCGGAT CCGGAAAGGG
    GTGTCTGCCT CAGCTGTGCC CTTCACTCCC TCCTCCCCGC TGCTGTCCTG CTCCCAGGAG
    GGAAGCCGCC ACACGAGCAA GCTTTCCCGC CACGGCAGTG GAGCCGACAG TGACTATGAG
    AACACGCAAA GTGGGGACCC ACTGCTGGGG CTGGAAGGGA AGAGGTTTCT AGAGCTGGGC
    AAAGAGGAAG ACTTCCACCC AGAGCTGGAA AGCCTGGATG GAGACCTAGA TCCTGGGCTT
    CCCAGCACAG AGGATGTCAT CTTGAAGACA GAGCAGGTCA CCAAGAACAT TCAGGAACTG
    TTGCGGGCAG CCCAGGAGTT CAAGCATGAC AGCTTCGTGC CCTGCTCAGA GAAGATCCAT
    TTGGCTGTGA CCGAGATGGC CTCCCTCTTC CCAAAGAGGC CAGCCCTGGA GCCAGTGCGG
    AGCTCACTGC GGCTGCTCAA CGCCAGCGCC TACCGGCTGC AGAGTGAGTG CCGGAAGACA
    GTGCCCCCAG AGCCCGGCGC CCCAGTGGAC TTCCAGCTGC TGACTCAGCA GGTGATCCAG
    TGCGCCTATG ACATCGCCAA GGCTGCCAAG CAGCTGGTCA CCATCACCAC CCGAGAGAAG
    AAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104394
    Clone ID Related Accession (Same CDS sequence) XM_024450733.1
    Accession Version XM_024450733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGCCT TGACTTATCC GAATTGGCCA AAGCTGCTAA GAAGAAGCTG
    CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTCGCCATGG ACGTGTATGA CGAGGTGGAT
    CGAAGAGAAA ATGATGCAGT GTGGCTGGCT ACCCAAAACC ACAGCACTCT GGTGACAGAG
    CGCAGTGCCG TGCCCTTCCT GCCTGTTAAC CCGGAATACT CAGCCACGCG GAATCAGGGG
    CGACAAAAGC TGGCCCGCTT TAATGCCCGA GAGTTTGCCA CCTTGATCAT CGACATTCTC
    AGTGAGGCCA AGCGGAGACA GCAGGGCAAG AGCCTGAGCA GCCCCACAGA CAACCTCGAG
    CTGTCTCTGC GGAGCCAGAG TGACCTCGAC GACCAACACG ACTACGACAG CGTGGCCTCT
    GACGAGGACA CAGACCAGGA GCCCCTGCGC AGCACCGGCG CCACTCGGAG CAACCGGGCC
    CGGAGCATGG ACTCCTCGGA CTTGTCTGAC GGGGCTGTGA CGCTGCAGGA GTACCTGGAG
    CTGAAGAAGG CCCTGGCTAC ATCGGAGGCA AAGGTGCAGC AGCTCATGAA GGTCAACAGT
    AGCCTGAGCG ACGAGCTCCG GAGGCTGCAG CGAGAGATCC ACAAGCTGCA GGCGGAGAAC
    CTGCAGCTCC GGCAGCCTCC AGGGCCGGTG CCCACACCTC CACTCCCCAG TGAACGGGCG
    GAACACACAC CCATGGCGCC AGGCGGGAGC ACACACCGCA GGGATCGCCA GGCCTTTTCC
    ATGTATGAAC CTGGCTCTGC CCTGAAGCCC TTTGGGGGCC CCCCTGGGGA CGAGCTCACT
    ACGCGGCTGC AGCCTTTCCA CAGCACTGAG CTAGAGGACG ACGCCATCTA TTCAGTGCAC
    GTCCCTGCTG GCCTTTACCG GATCCGGAAA GGGGTGTCTG CCTCAGCTGT GCCCTTCACT
    CCCTCCTCCC CGCTGCTGTC CTGCTCCCAG GAGGGAAGCC GCCACACGAG CAAGCTTTCC
    CGCCACGGCA GTGGAGCCGA CAGTGACTAT GAGAACACGC AAAGTGGGGA CCCACTGCTG
    GGGCTGGAAG GGAAGAGGTT TCTAGAGCTG GGCAAAGAGG AAGACTTCCA CCCAGAGCTG
    GAAAGCCTGG ATGGAGACCT AGATCCTGGG CTTCCCAGCA CAGAGGATGT CATCTTGAAG
    ACAGAGCAGG TCACCAAGAA CATTCAGGAA CTGTTGCGGG CAGCCCAGGA GTTCAAGCAT
    GACAGCTTCG TGCCCTGCTC AGAGAAGATC CATTTGGCTG TGACCGAGAT GGCCTCCCTC
    TTCCCAAAGC CTCACTCCTG CCCCCTCCAG AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA
    CTGCGGCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGTG AGTGCCGGAA GACAGTGCCC
    CCAGAGCCCG GCGCCCCAGT GGACTTCCAG CTGCTGACTC AGCAGGTGAT CCAGTGCGCC
    TATGACATCG CCAAGGCTGC CAAGCAGCTG GTCACCATCA CCACCCGAGA GAAGAAGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306501.1
    CDS16..2418
    Translation

    Target ORF information:

    RefSeq Version XM_024450733.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGCCT TGACTTATCC GAATTGGCCA AAGCTGCTAA GAAGAAGCTG
    CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTCGCCATGG ACGTGTATGA CGAGGTGGAT
    CGAAGAGAAA ATGATGCAGT GTGGCTGGCT ACCCAAAACC ACAGCACTCT GGTGACAGAG
    CGCAGTGCCG TGCCCTTCCT GCCTGTTAAC CCGGAATACT CAGCCACGCG GAATCAGGGG
    CGACAAAAGC TGGCCCGCTT TAATGCCCGA GAGTTTGCCA CCTTGATCAT CGACATTCTC
    AGTGAGGCCA AGCGGAGACA GCAGGGCAAG AGCCTGAGCA GCCCCACAGA CAACCTCGAG
    CTGTCTCTGC GGAGCCAGAG TGACCTCGAC GACCAACACG ACTACGACAG CGTGGCCTCT
    GACGAGGACA CAGACCAGGA GCCCCTGCGC AGCACCGGCG CCACTCGGAG CAACCGGGCC
    CGGAGCATGG ACTCCTCGGA CTTGTCTGAC GGGGCTGTGA CGCTGCAGGA GTACCTGGAG
    CTGAAGAAGG CCCTGGCTAC ATCGGAGGCA AAGGTGCAGC AGCTCATGAA GGTCAACAGT
    AGCCTGAGCG ACGAGCTCCG GAGGCTGCAG CGAGAGATCC ACAAGCTGCA GGCGGAGAAC
    CTGCAGCTCC GGCAGCCTCC AGGGCCGGTG CCCACACCTC CACTCCCCAG TGAACGGGCG
    GAACACACAC CCATGGCGCC AGGCGGGAGC ACACACCGCA GGGATCGCCA GGCCTTTTCC
    ATGTATGAAC CTGGCTCTGC CCTGAAGCCC TTTGGGGGCC CCCCTGGGGA CGAGCTCACT
    ACGCGGCTGC AGCCTTTCCA CAGCACTGAG CTAGAGGACG ACGCCATCTA TTCAGTGCAC
    GTCCCTGCTG GCCTTTACCG GATCCGGAAA GGGGTGTCTG CCTCAGCTGT GCCCTTCACT
    CCCTCCTCCC CGCTGCTGTC CTGCTCCCAG GAGGGAAGCC GCCACACGAG CAAGCTTTCC
    CGCCACGGCA GTGGAGCCGA CAGTGACTAT GAGAACACGC AAAGTGGGGA CCCACTGCTG
    GGGCTGGAAG GGAAGAGGTT TCTAGAGCTG GGCAAAGAGG AAGACTTCCA CCCAGAGCTG
    GAAAGCCTGG ATGGAGACCT AGATCCTGGG CTTCCCAGCA CAGAGGATGT CATCTTGAAG
    ACAGAGCAGG TCACCAAGAA CATTCAGGAA CTGTTGCGGG CAGCCCAGGA GTTCAAGCAT
    GACAGCTTCG TGCCCTGCTC AGAGAAGATC CATTTGGCTG TGACCGAGAT GGCCTCCCTC
    TTCCCAAAGC CTCACTCCTG CCCCCTCCAG AGGCCAGCCC TGGAGCCAGT GCGGAGCTCA
    CTGCGGCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGTG AGTGCCGGAA GACAGTGCCC
    CCAGAGCCCG GCGCCCCAGT GGACTTCCAG CTGCTGACTC AGCAGGTGAT CCAGTGCGCC
    TATGACATCG CCAAGGCTGC CAAGCAGCTG GTCACCATCA CCACCCGAGA GAAGAAGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104395
    Clone ID Related Accession (Same CDS sequence) XM_024450734.1
    Accession Version XM_024450734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ARF GTPase-activating protein GIT1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGCCT TGACTTATCC GAATTGGCCA AAGCTGCTAA GAAGAAGCTG
    CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTCGCCATGG ACGTGTATGA CGAGGTGGAT
    CGAAGAGAAA ATGATGCAGT GTGGCTGGCT ACCCAAAACC ACAGCACTCT GGTGACAGAG
    CGCAGTGCCG TGCCCTTCCT GCCTGTTAAC CCGGAATACT CAGCCACGCG GAATCAGGGG
    CGACAAAAGC TGGCCCGCTT TAATGCCCGA GAGTTTGCCA CCTTGATCAT CGACATTCTC
    AGTGAGGCCA AGCGGAGACA GCAGGGCAAG AGCCTGAGCA GCCCCACAGA CAACCTCGAG
    CTGTCTCTGC GGAGCCAGAG TGACCTCGAC GACCAACACG ACTACGACAG CGTGGCCTCT
    GACGAGGACA CAGACCAGGA GCCCCTGCGC AGCACCGGCG CCACTCGGAG CAACCGGGCC
    CGGAGCATGG ACTCCTCGGA CTTGTCTGAC GGGGCTGTGA CGCTGCAGGA GTACCTGGAG
    CTGAAGAAGG CCCTGGCTAC ATCGGAGGCA AAGGTGCAGC AGCTCATGAA GGTCAACAGT
    AGCCTGAGCG ACGAGCTCCG GAGGCTGCAG CGAGAGATCC ACAAGCTGCA GGCGGAGAAC
    CTGCAGCTCC GGCAGCCTCC AGGGCCGGTG CCCACACCTC CACTCCCCAG TGAACGGGCG
    GAACACACAC CCATGGCGCC AGGCGGGAGC ACACACCGCA GGGATCGCCA GGCCTTTTCC
    ATGTATGAAC CTGGCTCTGC CCTGAAGCCC TTTGGGGGCC CCCCTGGGGA CGAGCTCACT
    ACGCGGCTGC AGCCTTTCCA CAGCACTGAG CTAGAGGACG ACGCCATCTA TTCAGTGCAC
    GTCCCTGCTG GCCTTTACCG GATCCGGAAA GGGGTGTCTG CCTCAGCTGT GCCCTTCACT
    CCCTCCTCCC CGCTGCTGTC CTGCTCCCAG GAGGGAAGCC GCCACACGAG CAAGCTTTCC
    CGCCACGGCA GTGGAGCCGA CAGTGACTAT GAGAACACGC AAAGTGGGGA CCCACTGCTG
    GGGCTGGAAG GGAAGAGGTT TCTAGAGCTG GGCAAAGAGG AAGACTTCCA CCCAGAGCTG
    GAAAGCCTGG ATGGAGACCT AGATCCTGGG CTTCCCAGCA CAGAGGATGT CATCTTGAAG
    ACAGAGCAGG TCACCAAGAA CATTCAGGAA CTGTTGCGGG CAGCCCAGGA GTTCAAGCAT
    GACAGCTTCG TGCCCTGCTC AGAGAAGATC CATTTGGCTG TGACCGAGAT GGCCTCCCTC
    TTCCCAAAGA GGCCAGCCCT GGAGCCAGTG CGGAGCTCAC TGCGGCTGCT CAACGCCAGC
    GCCTACCGGC TGCAGAGTGA GTGCCGGAAG ACAGTGCCCC CAGAGCCCGG CGCCCCAGTG
    GACTTCCAGC TGCTGACTCA GCAGGTGATC CAGTGCGCCT ATGACATCGC CAAGGCTGCC
    AAGCAGCTGG TCACCATCAC CACCCGAGAG AAGAAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306502.1
    CDS16..2397
    Translation

    Target ORF information:

    RefSeq Version XM_024450734.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GIT ArfGAP 1 (GIT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCGAA AGGGGCCGCG AGCGGAGACC CTGGCTGGGC ATCCATCAGC AGGGGTGTGC 
    TGGTGTGTGA CGAGTGCTGC AGCGTGCACC GGAGCCTGGG ACGCCACATC TCCATTGTCA
    AGCACCTTCG CCACAGCGCC TGGCCTCCCA CGCTGCTGCA GATGGTGCAC ACGCTTGCCA
    GCAACGGGGC CAACTCCATC TGGGAGCACT CCCTGCTGGA CCCCGCACAA GTGCAGAGCG
    GCCGGCGTAA AGCCAACCCC CAAGACAAAG TCCAGTGAGT GGGGCTGGAG GGGTGGTGGG
    CAGAGACTGC GCAGTCCGGG ACAGGGCCCT GGGAGGTGGC AAGGTGACCA CCCTCTCCCA
    CCATGCTCCC CATTCCTCCT GTGTGCCCCA TGCAGCCCCA TCAAGTCAGA GTTCATCAGG
    GCCAAGTACC AGATGCTGGC ATTTGTGCAC AAGCTTCCCT GCCGGGACGA TGATGGAGTC
    ACCGCCAAAG ACCTCAGCAA GCAACTACAC TCGAGCGTGC GGACAGGCAA CCTGGAGACA
    TGTCTGCGCC TGCTCTCCCT GGGTGCCCAG GCCAACTTCT TCCACCCAGA GAAGGGCACC
    ACACCTCTGC ACGTGGCTGC CAAGGCAGGA CAGACACTGC AGGCCGAGCT GCTTGTAGTG
    TATGGGGCTG ACCCTGGCTC CCCTGATGTT AATGGCCGCA CACCCATTGA CTATGCCAGG
    CAGGCGGGGC ACCATGAGCT GGCGGAAAGG CTGGTTGAGT GCCAATATGA GCTCACTGAC
    CGGCTGGCCT TCTACCTCTG TGGACGCAAG CCGGATCACA AGAATGGGCA TTACATCATC
    CCACAGATGG CTGACAGCCT TGACTTATCC GAATTGGCCA AAGCTGCTAA GAAGAAGCTG
    CAGGCGCTCA GCAACCGGCT TTTTGAGGAA CTCGCCATGG ACGTGTATGA CGAGGTGGAT
    CGAAGAGAAA ATGATGCAGT GTGGCTGGCT ACCCAAAACC ACAGCACTCT GGTGACAGAG
    CGCAGTGCCG TGCCCTTCCT GCCTGTTAAC CCGGAATACT CAGCCACGCG GAATCAGGGG
    CGACAAAAGC TGGCCCGCTT TAATGCCCGA GAGTTTGCCA CCTTGATCAT CGACATTCTC
    AGTGAGGCCA AGCGGAGACA GCAGGGCAAG AGCCTGAGCA GCCCCACAGA CAACCTCGAG
    CTGTCTCTGC GGAGCCAGAG TGACCTCGAC GACCAACACG ACTACGACAG CGTGGCCTCT
    GACGAGGACA CAGACCAGGA GCCCCTGCGC AGCACCGGCG CCACTCGGAG CAACCGGGCC
    CGGAGCATGG ACTCCTCGGA CTTGTCTGAC GGGGCTGTGA CGCTGCAGGA GTACCTGGAG
    CTGAAGAAGG CCCTGGCTAC ATCGGAGGCA AAGGTGCAGC AGCTCATGAA GGTCAACAGT
    AGCCTGAGCG ACGAGCTCCG GAGGCTGCAG CGAGAGATCC ACAAGCTGCA GGCGGAGAAC
    CTGCAGCTCC GGCAGCCTCC AGGGCCGGTG CCCACACCTC CACTCCCCAG TGAACGGGCG
    GAACACACAC CCATGGCGCC AGGCGGGAGC ACACACCGCA GGGATCGCCA GGCCTTTTCC
    ATGTATGAAC CTGGCTCTGC CCTGAAGCCC TTTGGGGGCC CCCCTGGGGA CGAGCTCACT
    ACGCGGCTGC AGCCTTTCCA CAGCACTGAG CTAGAGGACG ACGCCATCTA TTCAGTGCAC
    GTCCCTGCTG GCCTTTACCG GATCCGGAAA GGGGTGTCTG CCTCAGCTGT GCCCTTCACT
    CCCTCCTCCC CGCTGCTGTC CTGCTCCCAG GAGGGAAGCC GCCACACGAG CAAGCTTTCC
    CGCCACGGCA GTGGAGCCGA CAGTGACTAT GAGAACACGC AAAGTGGGGA CCCACTGCTG
    GGGCTGGAAG GGAAGAGGTT TCTAGAGCTG GGCAAAGAGG AAGACTTCCA CCCAGAGCTG
    GAAAGCCTGG ATGGAGACCT AGATCCTGGG CTTCCCAGCA CAGAGGATGT CATCTTGAAG
    ACAGAGCAGG TCACCAAGAA CATTCAGGAA CTGTTGCGGG CAGCCCAGGA GTTCAAGCAT
    GACAGCTTCG TGCCCTGCTC AGAGAAGATC CATTTGGCTG TGACCGAGAT GGCCTCCCTC
    TTCCCAAAGA GGCCAGCCCT GGAGCCAGTG CGGAGCTCAC TGCGGCTGCT CAACGCCAGC
    GCCTACCGGC TGCAGAGTGA GTGCCGGAAG ACAGTGCCCC CAGAGCCCGG CGCCCCAGTG
    GACTTCCAGC TGCTGACTCA GCAGGTGATC CAGTGCGCCT ATGACATCGC CAAGGCTGCC
    AAGCAGCTGG TCACCATCAC CACCCGAGAG AAGAAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.