GPD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPD1
Entrez Gene ID	2819
Full Name	glycerol-3-phosphate dehydrogenase 1
Synonyms	GPD-C,GPDH-C,HTGTI
General protein information	Preferred Names glycerol-3-phosphate dehydrogenase 1 Names glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic glycerol-3-phosphate dehydrogenase 1 (soluble) glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic glycerophosphate dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:	138420
Disorder Html:	Hypertriglyceridemia, transient infantile, 614480 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001257199.2	NP_001244128.1	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 2
NM_005276.4	NP_005267.2	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 1
NM_005276.3	NP_005267.2	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 1
NM_001257199.1	NP_001244128.1	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPD1	NP_005267.2
Canis lupus familiaris (dog)	GPD1	XP_850380.1
Rattus norvegicus (Norway rat)	Gpd1	NP_071551.2
Danio rerio (zebrafish)	gpd1b	NP_956000.1
Mus musculus (house mouse)	Gpd1	NP_034401.1
Saccharomyces cerevisiae (baker's yeast)	GPD1	NP_010262.1
Saccharomyces cerevisiae (baker's yeast)	GPD2	NP_014582.1
Schizosaccharomyces pombe (fission yeast)	gpd2	NP_594542.1
Neurospora crassa	NCU00742	XP_964395.2
Xenopus tropicalis (tropical clawed frog)	gpd1	NP_001006808.1
Pan troglodytes (chimpanzee)	GPD1	XP_001154939.1
Bos taurus (cattle)	GPD1	NP_001030431.1
Gallus gallus (chicken)	LOC426881	XP_003643165.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007593	XP_001687881.1
Magnaporthe oryzae (rice blast fungus)	MGG_00067	XP_003719075.1
Danio rerio (zebrafish)	gpd1a	NP_001017709.1
Drosophila melanogaster (fruit fly)	Gpdh	NP_476566.1
Caenorhabditis elegans (roundworm)	gpdh-2	NP_499188.2
	GPD1	XP_001102726.2
Kluyveromyces lactis	KLLA0C04004g	XP_452375.1
Eremothecium gossypii	AGOS_ADR311C	NP_984407.1
Schizosaccharomyces pombe (fission yeast)	gpd1	NP_596682.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycerol-3-phosphate dehydrogenase 1 deficiency induces compensatory amino acid metabolism during fasting in mice.
Sato T, Yoshida Y, Morita A, Mori N, Miura S
Metabolism: clinical and experimental65(11)1646-1656(2016 Nov)

Expanding the molecular diversity and phenotypic spectrum of glycerol 3-phosphate dehydrogenase 1 deficiency.
Dionisi-Vici C, Shteyer E, Niceta M, Rizzo C, Pode-Shakked B, Chillemi G, Bruselles A, Semeraro M, Barel O, Eyal E, Kol N, Haberman Y, Lahad A, Diomedi-Camassei F, Marek-Yagel D, Rechavi G, Tartaglia M, Anikster Y
Journal of inherited metabolic disease39(5)689-695(2016 Sep)

Enzyme architecture: optimization of transition state stabilization from a cation-phosphodianion pair.
Reyes AC, Koudelka AP, Amyes TL, Richard JP
Journal of the American Chemical Society137(16)5312-5(2015 Apr)

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, Eagan J, Desai NK, Newton SA, Towne MC, Marinakis NS, Esteves KM, De Ferranti S, Bennett MJ, McIntyre A, Beggs AH, Berry GT, Agrawal PB
European journal of human genetics : EJHG22(10)1229-32(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although these data indicate that a lack of GPD1 inhibits gluconeogenesis from glycerol, chronic GPD1 deficiency may induce an adaptation that enhances gluconeogenesis from glycogenic amino acids
Title: Glycerol-3-phosphate dehydrogenase 1 deficiency induces compensatory amino acid metabolism during fasting in mice.

To recognize this entity by considering GPD1 mutations in appropriate clinical settings.
Title: Expanding the molecular diversity and phenotypic spectrum of glycerol 3-phosphate dehydrogenase 1 deficiency.

The R269A mutation of GPDH1 results in a 110-fold increase in K(m) (2.8 kcal/mol effect) and a 41,000-fold decrease in k(cat) (6.3 kcal/mol effect), which corresponds to a 9.1 kcal/mol destabilization of the transition state.
Title: Enzyme architecture: optimization of transition state stabilization from a cation-phosphodianion pair.

massive hepatomegaly, fatty liver and severe hypertriglyceridemia carrying a compound heterozygous mutation in GPD1
Title: A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.

Mutation analysis revealed a homozygous splicing mutation, c.361-1G>C, which resulted in an aberrantly spliced mRNA in the ten affected individuals.
Title: Transient infantile hypertriglyceridemia, fatty liver, and hepatic fibrosis caused by mutated GPD1, encoding glycerol-3-phosphate dehydrogenase 1.

The following GPD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20325	NM_005276.4 Latest version!	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09605	NM_001257199.2 Latest version!	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu20325	NM_005276.3	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09605	NM_001257199.1	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPD1 ( NM_005276.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005267.2 Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20325
Clone ID Related Accession (Same CDS sequence)	NM_005276.4 , NM_005276.3
Accession Version	NM_005276.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC305085.1, BC032234.1, AK130162.1, CD244782.1, CA307120.1 and AI245302.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005276.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032234.1, SRR1803617.182291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301149.8/ ENSP00000301149.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGG TGGCTGTCCC AGATGTGGTC 
CAGGCTGCAG AGGATGCTGA CATCCTGATC TTTGTGGTGC CCCATCAGTT CATCGGCAAG 
ATCTGTGACC AGCTCAAGGG CCATCTGAAG GCAAACGCCA CTGGCATATC TCTTATTAAG 
GGGGTAGACG AGGGCCCCAA TGGGCTGAAG CTCATCTCGG AAGTGATTGG GGAGCGCCTC 
GGCATCCCCA TGAGTGTGCT GATGGGGGCC AACATTGCCA GCGAGGTGGC TGATGAGAAG 
TTCTGTGAGA CAACCATTGG CTGCAAGGAC CCGGCCCAGG GACAACTCCT GAAAGAGCTG 
ATGCAGACAC CAAACTTCCG TATCACAGTG GTGCAAGAGG TGGACACAGT AGAGATCTGT 
GGAGCCTTAA AGAATGTAGT GGCCGTGGGG GCTGGCTTCT GTGATGGCCT GGGCTTTGGC 
GACAACACCA AGGCGGCAGT GATCCGGCTG GGACTCATGG AGATGATAGC CTTCGCCAAG 
CTCTTCTGCA GTGGCCCTGT GTCCTCTGCC ACCTTCTTGG AGAGCTGTGG TGTTGCTGAC 
CTGATCACTA CCTGCTATGG AGGGCGGAAC CGGAAAGTGG CTGAGGCCTT TGCGCGTACA 
GGAAAGTCCA TTGAGCAGCT GGAGAAAGAG TTGCTGAATG GGCAGAAACT GCAGGGGCCC 
GAGACAGCCC GGGAGCTATA CAGCATCCTC CAGCACAAGG GCCTGGTAGA CAAGTTTCCC 
TTGTTCATGG CTGTGTACAA GGTGTGCTAC GAGGGCCAGC CAGTGGGTGA ATTCATCCAC 
TGCCTGCAGA ATCATCCAGA ACATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005267.2
CDS	44..1093
Translation	MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

Target ORF information:

RefSeq Version	NM_005276.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005276.4

ORF Insert Sequence:

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGG TGGCTGTCCC AGATGTGGTC 
CAGGCTGCAG AGGATGCTGA CATCCTGATC TTTGTGGTGC CCCATCAGTT CATCGGCAAG 
ATCTGTGACC AGCTCAAGGG CCATCTGAAG GCAAACGCCA CTGGCATATC TCTTATTAAG 
GGGGTAGACG AGGGCCCCAA TGGGCTGAAG CTCATCTCGG AAGTGATTGG GGAGCGCCTC 
GGCATCCCCA TGAGTGTGCT GATGGGGGCC AACATTGCCA GCGAGGTGGC TGATGAGAAG 
TTCTGTGAGA CAACCATTGG CTGCAAGGAC CCGGCCCAGG GACAACTCCT GAAAGAGCTG 
ATGCAGACAC CAAACTTCCG TATCACAGTG GTGCAAGAGG TGGACACAGT AGAGATCTGT 
GGAGCCTTAA AGAATGTAGT GGCCGTGGGG GCTGGCTTCT GTGATGGCCT GGGCTTTGGC 
GACAACACCA AGGCGGCAGT GATCCGGCTG GGACTCATGG AGATGATAGC CTTCGCCAAG 
CTCTTCTGCA GTGGCCCTGT GTCCTCTGCC ACCTTCTTGG AGAGCTGTGG TGTTGCTGAC 
CTGATCACTA CCTGCTATGG AGGGCGGAAC CGGAAAGTGG CTGAGGCCTT TGCGCGTACA 
GGAAAGTCCA TTGAGCAGCT GGAGAAAGAG TTGCTGAATG GGCAGAAACT GCAGGGGCCC 
GAGACAGCCC GGGAGCTATA CAGCATCCTC CAGCACAAGG GCCTGGTAGA CAAGTTTCCC 
TTGTTCATGG CTGTGTACAA GGTGTGCTAC GAGGGCCAGC CAGTGGGTGA ATTCATCCAC 
TGCCTGCAGA ATCATCCAGA ACATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPD1 ( NM_005276.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005267.2 Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20325
Clone ID Related Accession (Same CDS sequence)	NM_005276.4 , NM_005276.3
Accession Version	NM_005276.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC310521.1, DC305085.1, BC032234.1, AK130162.1, CD244782.1, CA307120.1 and AI245302.1. This sequence is a reference standard in the RefSeqGene project. On Mar 20, 2012 this sequence version replaced NM_005276.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032234.1, SRR1803617.182291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGG TGGCTGTCCC AGATGTGGTC 
CAGGCTGCAG AGGATGCTGA CATCCTGATC TTTGTGGTGC CCCATCAGTT CATCGGCAAG 
ATCTGTGACC AGCTCAAGGG CCATCTGAAG GCAAACGCCA CTGGCATATC TCTTATTAAG 
GGGGTAGACG AGGGCCCCAA TGGGCTGAAG CTCATCTCGG AAGTGATTGG GGAGCGCCTC 
GGCATCCCCA TGAGTGTGCT GATGGGGGCC AACATTGCCA GCGAGGTGGC TGATGAGAAG 
TTCTGTGAGA CAACCATTGG CTGCAAGGAC CCGGCCCAGG GACAACTCCT GAAAGAGCTG 
ATGCAGACAC CAAACTTCCG TATCACAGTG GTGCAAGAGG TGGACACAGT AGAGATCTGT 
GGAGCCTTAA AGAATGTAGT GGCCGTGGGG GCTGGCTTCT GTGATGGCCT GGGCTTTGGC 
GACAACACCA AGGCGGCAGT GATCCGGCTG GGACTCATGG AGATGATAGC CTTCGCCAAG 
CTCTTCTGCA GTGGCCCTGT GTCCTCTGCC ACCTTCTTGG AGAGCTGTGG TGTTGCTGAC 
CTGATCACTA CCTGCTATGG AGGGCGGAAC CGGAAAGTGG CTGAGGCCTT TGCGCGTACA 
GGAAAGTCCA TTGAGCAGCT GGAGAAAGAG TTGCTGAATG GGCAGAAACT GCAGGGGCCC 
GAGACAGCCC GGGAGCTATA CAGCATCCTC CAGCACAAGG GCCTGGTAGA CAAGTTTCCC 
TTGTTCATGG CTGTGTACAA GGTGTGCTAC GAGGGCCAGC CAGTGGGTGA ATTCATCCAC 
TGCCTGCAGA ATCATCCAGA ACATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005267.2
CDS	233..1282
Translation	MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

Target ORF information:

RefSeq Version	NM_005276.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005276.3

ORF Insert Sequence:

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGG TGGCTGTCCC AGATGTGGTC 
CAGGCTGCAG AGGATGCTGA CATCCTGATC TTTGTGGTGC CCCATCAGTT CATCGGCAAG 
ATCTGTGACC AGCTCAAGGG CCATCTGAAG GCAAACGCCA CTGGCATATC TCTTATTAAG 
GGGGTAGACG AGGGCCCCAA TGGGCTGAAG CTCATCTCGG AAGTGATTGG GGAGCGCCTC 
GGCATCCCCA TGAGTGTGCT GATGGGGGCC AACATTGCCA GCGAGGTGGC TGATGAGAAG 
TTCTGTGAGA CAACCATTGG CTGCAAGGAC CCGGCCCAGG GACAACTCCT GAAAGAGCTG 
ATGCAGACAC CAAACTTCCG TATCACAGTG GTGCAAGAGG TGGACACAGT AGAGATCTGT 
GGAGCCTTAA AGAATGTAGT GGCCGTGGGG GCTGGCTTCT GTGATGGCCT GGGCTTTGGC 
GACAACACCA AGGCGGCAGT GATCCGGCTG GGACTCATGG AGATGATAGC CTTCGCCAAG 
CTCTTCTGCA GTGGCCCTGT GTCCTCTGCC ACCTTCTTGG AGAGCTGTGG TGTTGCTGAC 
CTGATCACTA CCTGCTATGG AGGGCGGAAC CGGAAAGTGG CTGAGGCCTT TGCGCGTACA 
GGAAAGTCCA TTGAGCAGCT GGAGAAAGAG TTGCTGAATG GGCAGAAACT GCAGGGGCCC 
GAGACAGCCC GGGAGCTATA CAGCATCCTC CAGCACAAGG GCCTGGTAGA CAAGTTTCCC 
TTGTTCATGG CTGTGTACAA GGTGTGCTAC GAGGGCCAGC CAGTGGGTGA ATTCATCCAC 
TGCCTGCAGA ATCATCCAGA ACATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPD1 ( NM_001257199.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244128.1 Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09605
Clone ID Related Accession (Same CDS sequence)	NM_001257199.2 , NM_001257199.1
Accession Version	NM_001257199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK130162.1, CD244782.1, CA307120.1 and AI245302.1. On May 31, 2019 this sequence version replaced NM_001257199.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGT TCATCGGCAA GATCTGTGAC 
CAGCTCAAGG GCCATCTGAA GGCAAACGCC ACTGGCATAT CTCTTATTAA GGGGGTAGAC 
GAGGGCCCCA ATGGGCTGAA GCTCATCTCG GAAGTGATTG GGGAGCGCCT CGGCATCCCC 
ATGAGTGTGC TGATGGGGGC CAACATTGCC AGCGAGGTGG CTGATGAGAA GTTCTGTGAG 
ACAACCATTG GCTGCAAGGA CCCGGCCCAG GGACAACTCC TGAAAGAGCT GATGCAGACA 
CCAAACTTCC GTATCACAGT GGTGCAAGAG GTGGACACAG TAGAGATCTG TGGAGCCTTA 
AAGAATGTAG TGGCCGTGGG GGCTGGCTTC TGTGATGGCC TGGGCTTTGG CGACAACACC 
AAGGCGGCAG TGATCCGGCT GGGACTCATG GAGATGATAG CCTTCGCCAA GCTCTTCTGC 
AGTGGCCCTG TGTCCTCTGC CACCTTCTTG GAGAGCTGTG GTGTTGCTGA CCTGATCACT 
ACCTGCTATG GAGGGCGGAA CCGGAAAGTG GCTGAGGCCT TTGCGCGTAC AGGAAAGTCC 
ATTGAGCAGC TGGAGAAAGA GTTGCTGAAT GGGCAGAAAC TGCAGGGGCC CGAGACAGCC 
CGGGAGCTAT ACAGCATCCT CCAGCACAAG GGCCTGGTAG ACAAGTTTCC CTTGTTCATG 
GCTGTGTACA AGGTGTGCTA CGAGGGCCAG CCAGTGGGTG AATTCATCCA CTGCCTGCAG 
AATCATCCAG AACATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244128.1
CDS	44..1024
Translation	MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

Target ORF information:

RefSeq Version	NM_001257199.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257199.2

ORF Insert Sequence:

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGT TCATCGGCAA GATCTGTGAC 
CAGCTCAAGG GCCATCTGAA GGCAAACGCC ACTGGCATAT CTCTTATTAA GGGGGTAGAC 
GAGGGCCCCA ATGGGCTGAA GCTCATCTCG GAAGTGATTG GGGAGCGCCT CGGCATCCCC 
ATGAGTGTGC TGATGGGGGC CAACATTGCC AGCGAGGTGG CTGATGAGAA GTTCTGTGAG 
ACAACCATTG GCTGCAAGGA CCCGGCCCAG GGACAACTCC TGAAAGAGCT GATGCAGACA 
CCAAACTTCC GTATCACAGT GGTGCAAGAG GTGGACACAG TAGAGATCTG TGGAGCCTTA 
AAGAATGTAG TGGCCGTGGG GGCTGGCTTC TGTGATGGCC TGGGCTTTGG CGACAACACC 
AAGGCGGCAG TGATCCGGCT GGGACTCATG GAGATGATAG CCTTCGCCAA GCTCTTCTGC 
AGTGGCCCTG TGTCCTCTGC CACCTTCTTG GAGAGCTGTG GTGTTGCTGA CCTGATCACT 
ACCTGCTATG GAGGGCGGAA CCGGAAAGTG GCTGAGGCCT TTGCGCGTAC AGGAAAGTCC 
ATTGAGCAGC TGGAGAAAGA GTTGCTGAAT GGGCAGAAAC TGCAGGGGCC CGAGACAGCC 
CGGGAGCTAT ACAGCATCCT CCAGCACAAG GGCCTGGTAG ACAAGTTTCC CTTGTTCATG 
GCTGTGTACA AGGTGTGCTA CGAGGGCCAG CCAGTGGGTG AATTCATCCA CTGCCTGCAG 
AATCATCCAG AACATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPD1 ( NM_001257199.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244128.1 Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.

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CloneID	OHu09605
Clone ID Related Accession (Same CDS sequence)	NM_001257199.2 , NM_001257199.1
Accession Version	NM_001257199.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC310521.1, DC305085.1, AK130162.1, CD244782.1, CA307120.1 and AI245302.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGT TCATCGGCAA GATCTGTGAC 
CAGCTCAAGG GCCATCTGAA GGCAAACGCC ACTGGCATAT CTCTTATTAA GGGGGTAGAC 
GAGGGCCCCA ATGGGCTGAA GCTCATCTCG GAAGTGATTG GGGAGCGCCT CGGCATCCCC 
ATGAGTGTGC TGATGGGGGC CAACATTGCC AGCGAGGTGG CTGATGAGAA GTTCTGTGAG 
ACAACCATTG GCTGCAAGGA CCCGGCCCAG GGACAACTCC TGAAAGAGCT GATGCAGACA 
CCAAACTTCC GTATCACAGT GGTGCAAGAG GTGGACACAG TAGAGATCTG TGGAGCCTTA 
AAGAATGTAG TGGCCGTGGG GGCTGGCTTC TGTGATGGCC TGGGCTTTGG CGACAACACC 
AAGGCGGCAG TGATCCGGCT GGGACTCATG GAGATGATAG CCTTCGCCAA GCTCTTCTGC 
AGTGGCCCTG TGTCCTCTGC CACCTTCTTG GAGAGCTGTG GTGTTGCTGA CCTGATCACT 
ACCTGCTATG GAGGGCGGAA CCGGAAAGTG GCTGAGGCCT TTGCGCGTAC AGGAAAGTCC 
ATTGAGCAGC TGGAGAAAGA GTTGCTGAAT GGGCAGAAAC TGCAGGGGCC CGAGACAGCC 
CGGGAGCTAT ACAGCATCCT CCAGCACAAG GGCCTGGTAG ACAAGTTTCC CTTGTTCATG 
GCTGTGTACA AGGTGTGCTA CGAGGGCCAG CCAGTGGGTG AATTCATCCA CTGCCTGCAG 
AATCATCCAG AACATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244128.1
CDS	233..1213
Translation	MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

Target ORF information:

RefSeq Version	NM_001257199.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerol-3-phosphate dehydrogenase 1 (GPD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257199.1

ORF Insert Sequence:

ATGGCTAGCA AGAAAGTCTG CATTGTAGGC TCCGGGAACT GGGGCTCAGC CATCGCCAAG 
ATCGTGGGTG GCAATGCAGC CCAGCTGGCA CAGTTTGACC CACGGGTGAC CATGTGGGTA 
TTTGAGGAAG ACATTGGAGG CAAAAAGCTG ACTGAGATCA TCAACACGCA GCATGAGAAT 
GTCAAATACC TGCCAGGGCA CAAGTTGCCC CCAAATGTGT TCATCGGCAA GATCTGTGAC 
CAGCTCAAGG GCCATCTGAA GGCAAACGCC ACTGGCATAT CTCTTATTAA GGGGGTAGAC 
GAGGGCCCCA ATGGGCTGAA GCTCATCTCG GAAGTGATTG GGGAGCGCCT CGGCATCCCC 
ATGAGTGTGC TGATGGGGGC CAACATTGCC AGCGAGGTGG CTGATGAGAA GTTCTGTGAG 
ACAACCATTG GCTGCAAGGA CCCGGCCCAG GGACAACTCC TGAAAGAGCT GATGCAGACA 
CCAAACTTCC GTATCACAGT GGTGCAAGAG GTGGACACAG TAGAGATCTG TGGAGCCTTA 
AAGAATGTAG TGGCCGTGGG GGCTGGCTTC TGTGATGGCC TGGGCTTTGG CGACAACACC 
AAGGCGGCAG TGATCCGGCT GGGACTCATG GAGATGATAG CCTTCGCCAA GCTCTTCTGC 
AGTGGCCCTG TGTCCTCTGC CACCTTCTTG GAGAGCTGTG GTGTTGCTGA CCTGATCACT 
ACCTGCTATG GAGGGCGGAA CCGGAAAGTG GCTGAGGCCT TTGCGCGTAC AGGAAAGTCC 
ATTGAGCAGC TGGAGAAAGA GTTGCTGAAT GGGCAGAAAC TGCAGGGGCC CGAGACAGCC 
CGGGAGCTAT ACAGCATCCT CCAGCACAAG GGCCTGGTAG ACAAGTTTCC CTTGTTCATG 
GCTGTGTACA AGGTGTGCTA CGAGGGCCAG CCAGTGGGTG AATTCATCCA CTGCCTGCAG 
AATCATCCAG AACATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycerol-3-phosphate dehydrogenase 1 deficiency induces compensatory amino acid metabolism during fasting in mice.
Metabolism: clinical and experimental65(11)1646-1656(2016 Nov)
Sato T,Yoshida Y,Morita A,Mori N,Miura S

Expanding the molecular diversity and phenotypic spectrum of glycerol 3-phosphate dehydrogenase 1 deficiency.
Journal of inherited metabolic disease39(5)689-695(2016 Sep)
Dionisi-Vici C,Shteyer E,Niceta M,Rizzo C,Pode-Shakked B,Chillemi G,Bruselles A,Semeraro M,Barel O,Eyal E,Kol N,Haberman Y,Lahad A,Diomedi-Camassei F,Marek-Yagel D,Rechavi G,Tartaglia M,Anikster Y

Enzyme architecture: optimization of transition state stabilization from a cation-phosphodianion pair.
Journal of the American Chemical Society137(16)5312-5(2015 Apr)
Reyes AC,Koudelka AP,Amyes TL,Richard JP

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
European journal of human genetics : EJHG22(10)1229-32(2014 Oct)
Joshi M,Eagan J,Desai NK,Newton SA,Towne MC,Marinakis NS,Esteves KM,De Ferranti S,Bennett MJ,McIntyre A,Beggs AH,Berry GT,Agrawal PB

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GPD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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