GNGT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNGT1
Entrez Gene ID	2792
Full Name	G protein subunit gamma transducin 1
Synonyms	GNG1
General protein information	Preferred Names G protein subunit gamma transducin 1 Names guanine nucleotide-binding protein G(T) subunit gamma-T1 guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 transducin gamma chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.3
Summary	This gene encodes the gamma subunit of transducin, a guanine nucleotide-binding protein (G protein) that is found in rod outer segments. Transducin, also known as GMPase, mediates the activation of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase by rhodopsin. [provided by RefSeq, Jul 2016].
Disorder MIM:	189970

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329426.1	NP_001316355.1	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
NM_021955.5	NP_068774.1	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
NM_001329426.2	NP_001316355.1	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
NM_021955.4	NP_068774.1	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gngt1	NP_001129249.1
Homo sapiens (human)	GNGT1	NP_068774.1
Bos taurus (cattle)	GNGT1	NP_776752.1
Mus musculus (house mouse)	Gngt1	NP_034444.1
	GNGT1	XP_001098610.2
Xenopus tropicalis (tropical clawed frog)	gngt1	NP_001165112.1
Danio rerio (zebrafish)	gngt1	NP_956261.1
Pan troglodytes (chimpanzee)	GNGT1	XP_001168330.1
Canis lupus familiaris (dog)	GNGT1	NP_001003225.1

Gene Ontology
Bibliography

Related articles in PubMed

G?4?1 as a modulator of M3 muscarinic receptor signalling and novel roles of G?1 subunits in the modulation of cellular signalling.
Khan SM, Min A, Gora S, Houranieh GM, Campden R, Robitaille M, Trieu P, P?trin D, Jacobi AM, Behlke MA, Angers S, H?bert TE
Cellular signalling27(8)1597-608(2015 Aug)

Structures of the G?-CCT and PhLP1-G?-CCT complexes reveal a mechanism for G-protein ?-subunit folding and G?? dimer assembly.
Plimpton RL, Cu?llar J, Lai CW, Aoba T, Makaju A, Franklin S, Mathis AD, Prince JT, Carrascosa JL, Valpuesta JM, Willardson BM
Proceedings of the National Academy of Sciences of the United States of America112(8)2413-8(2015 Feb)

IGF-1 activates a cilium-localized noncanonical G?? signaling pathway that regulates cell-cycle progression.
Yeh C, Li A, Chuang JZ, Saito M, C?ceres A, Sung CH
Developmental cell26(4)358-68(2013 Aug)

Genome-wide association study of lung function decline in adults with and without asthma.
Imboden M, Bouzigon E, Curjuric I, Ramasamy A, Kumar A, Hancock DB, Wilk JB, Vonk JM, Thun GA, Siroux V, Nadif R, Monier F, Gonzalez JR, Wjst M, Heinrich J, Loehr LR, Franceschini N, North KE, Altm?ller J, Koppelman GH, Guerra S, Kronenberg F, Lathrop M, Moffatt MF, O'Connor GT, Strachan DP, Postma DS, London SJ, Schindler C, Kogevinas M, Kauffmann F, Jarvis DL, Demenais F, Probst-Hensch NM
The Journal of allergy and clinical immunology129(5)1218-28(2012 May)

The WD40 repeat protein WDR26 binds G?? and promotes G??-dependent signal transduction and leukocyte migration.
Sun Z, Tang X, Lin F, Chen S
The Journal of biological chemistry286(51)43902-12(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest Gbeta4gamma1 as a modulator of M3 muscarinic receptor signaling.
Title: G?4?1 as a modulator of M3 muscarinic receptor signalling and novel roles of G?1 subunits in the modulation of cellular signalling.

PhLP1 binding stabilizes the Gbeta fold, disrupting interactions with CCT and releasing a PhLP1-Gbeta dimer for assembly with Ggamma.
Title: Structures of the G?-CCT and PhLP1-G?-CCT complexes reveal a mechanism for G-protein ?-subunit folding and G?? dimer assembly.

During corticogenesis, a cilium-transduced, noncanonical IGF-1R-Gbetagamma-phospho(T94)Tctex-1 signaling pathway promotes the proliferation of neural progenitors through modulation of ciliary resorption and G1 length.
Title: IGF-1 activates a cilium-localized noncanonical G?? signaling pathway that regulates cell-cycle progression.

WDR26 is a novel Gbetagamma-binding protein that is required for the efficacy of Gbetagamma signaling and leukocyte migration
Title: The WD40 repeat protein WDR26 binds G?? and promotes G??-dependent signal transduction and leukocyte migration.

Gbetagamma subunits enter in a protein complex with activated Rap1a and its effector Radil; this complex is required downstream of receptor stimulation for the activation of integrins and the positive modulation of cell-matrix adhesiveness.
Title: G protein betagamma subunits regulate cell adhesion through Rap1a and its effector Radil.

The following GNGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04853	NM_001329426.2 Latest version!	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu04853	NM_021955.5 Latest version!	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu04853	NM_001329426.1	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu04853	NM_021955.4	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNGT1 ( NM_001329426.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316355.1 Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04853
Clone ID Related Accession (Same CDS sequence)	NM_001329426.1 , NM_021955.5 , NM_001329426.2 , NM_021955.4
Accession Version	NM_001329426.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG435040.1, EL950965.1 and BQ637754.1. On Jul 12, 2016 this sequence version replaced XM_017012009.1. Transcript Variant: This variant (2) encodes the same protein as variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EL950965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316355.1
CDS	146..370
Translation	MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS

Target ORF information:

RefSeq Version	NM_001329426.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329426.1

ORF Insert Sequence:

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNGT1 ( NM_021955.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_068774.1 Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04853
Clone ID Related Accession (Same CDS sequence)	NM_001329426.1 , NM_021955.5 , NM_001329426.2 , NM_021955.4
Accession Version	NM_021955.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC029367.1 and BQ637754.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_021955.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ637754.1, BX509917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248572.10/ ENSP00000248572.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068774.1
CDS	90..314
Translation	MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS

Target ORF information:

RefSeq Version	NM_021955.5
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021955.5

ORF Insert Sequence:

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNGT1 ( NM_001329426.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316355.1 Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04853
Clone ID Related Accession (Same CDS sequence)	NM_001329426.1 , NM_021955.5 , NM_001329426.2 , NM_021955.4
Accession Version	NM_001329426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG435040.1, EL950965.1 and BQ637754.1. On May 31, 2019 this sequence version replaced NM_001329426.1. Transcript Variant: This variant (2) encodes the same protein as variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EL950965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316355.1
CDS	87..311
Translation	MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS

Target ORF information:

RefSeq Version	NM_001329426.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329426.2

ORF Insert Sequence:

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNGT1 ( NM_021955.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068774.1 Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04853
Clone ID Related Accession (Same CDS sequence)	NM_001329426.1 , NM_021955.5 , NM_001329426.2 , NM_021955.4
Accession Version	NM_021955.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC029367.1 and BQ637754.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2016 this sequence version replaced NM_021955.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ637754.1, EL946418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068774.1
CDS	149..373
Translation	MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS

Target ORF information:

RefSeq Version	NM_021955.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma transducin 1 (GNGT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021955.4

ORF Insert Sequence:

ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA 
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC 
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

G?4?1 as a modulator of M3 muscarinic receptor signalling and novel roles of G?1 subunits in the modulation of cellular signalling.
Cellular signalling27(8)1597-608(2015 Aug)
Khan SM,Min A,Gora S,Houranieh GM,Campden R,Robitaille M,Trieu P,P?trin D,Jacobi AM,Behlke MA,Angers S,H?bert TE

Structures of the G?-CCT and PhLP1-G?-CCT complexes reveal a mechanism for G-protein ?-subunit folding and G?? dimer assembly.
Proceedings of the National Academy of Sciences of the United States of America112(8)2413-8(2015 Feb)
Plimpton RL,Cu?llar J,Lai CW,Aoba T,Makaju A,Franklin S,Mathis AD,Prince JT,Carrascosa JL,Valpuesta JM,Willardson BM

IGF-1 activates a cilium-localized noncanonical G?? signaling pathway that regulates cell-cycle progression.
Developmental cell26(4)358-68(2013 Aug)
Yeh C,Li A,Chuang JZ,Saito M,C?ceres A,Sung CH

Genome-wide association study of lung function decline in adults with and without asthma.
The Journal of allergy and clinical immunology129(5)1218-28(2012 May)
Imboden M,Bouzigon E,Curjuric I,Ramasamy A,Kumar A,Hancock DB,Wilk JB,Vonk JM,Thun GA,Siroux V,Nadif R,Monier F,Gonzalez JR,Wjst M,Heinrich J,Loehr LR,Franceschini N,North KE,Altm?ller J,Koppelman GH,Guerra S,Kronenberg F,Lathrop M,Moffatt MF,O'Connor GT,Strachan DP,Postma DS,London SJ,Schindler C,Kogevinas M,Kauffmann F,Jarvis DL,Demenais F,Probst-Hensch NM

The WD40 repeat protein WDR26 binds G?? and promotes G??-dependent signal transduction and leukocyte migration.
The Journal of biological chemistry286(51)43902-12(2011 Dec)
Sun Z,Tang X,Lin F,Chen S

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GNGT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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