AMT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMT
Entrez Gene ID	275
Full Name	aminomethyltransferase
Synonyms	GCE,GCST,GCVT,NKH
General protein information	Preferred Names aminomethyltransferase Names aminomethyltransferase, mitochondrial glycine cleavage system T protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Disorder MIM:	238310
Disorder Html:	Glycine encephalopathy, 605899 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164712.1	NP_001158184.1	aminomethyltransferase, mitochondrial isoform 4 precursor
NM_001164712.2	NP_001158184.1	aminomethyltransferase, mitochondrial isoform 4 precursor
NM_001164711.1	NP_001158183.1	aminomethyltransferase, mitochondrial isoform 3 precursor
NM_001164710.2	NP_001158182.1	aminomethyltransferase, mitochondrial isoform 2 precursor
NM_000481.3	NP_000472.2	aminomethyltransferase, mitochondrial isoform 1 precursor
NM_001164711.2	NP_001158183.1	aminomethyltransferase, mitochondrial isoform 3 precursor
NM_000481.4	NP_000472.2	aminomethyltransferase, mitochondrial isoform 1 precursor
NM_001164710.1	NP_001158182.1	aminomethyltransferase, mitochondrial isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Amt	NP_001013836.1
Gallus gallus (chicken)	AMT	NP_990119.1
Pan troglodytes (chimpanzee)	AMT	XP_517018.2
Caenorhabditis elegans (roundworm)	CELE_F25B4.1	NP_504502.1
Saccharomyces cerevisiae (baker's yeast)	GCV1	NP_010302.1
Magnaporthe oryzae (rice blast fungus)	MGG_04826	XP_003713755.1
Xenopus tropicalis (tropical clawed frog)	amt	XP_002936519.1
	AMT	XP_001108852.1
Drosophila melanogaster (fruit fly)	CG6415	NP_609441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001124	XP_322034.3
Schizosaccharomyces pombe (fission yeast)	gcv1	NP_594015.1
Neurospora crassa	NCU02727	XP_965112.1
Canis lupus familiaris (dog)	AMT	NP_001029165.1
Bos taurus (cattle)	AMT	NP_803451.1
Rattus norvegicus (Norway rat)	Amt	NP_001014026.1
Arabidopsis thaliana (thale cress)	AT1G11860	NP_001184969.1
Oryza sativa (rice)	Os04g0623800	NP_001053928.1
Homo sapiens (human)	AMT	NP_000472.2
Danio rerio (zebrafish)	amt	NP_001006021.1
Kluyveromyces lactis	KLLA0B06754g	XP_451833.1

Gene Ontology
Bibliography

Related articles in PubMed

The genetic basis of classic nonketotic hyperglycinemia due to mutations in GLDC and AMT.
Coughlin CR 2nd, Swanson MA, Kronquist K, Acquaviva C, Hutchin T, Rodr?guez-Pombo P, V?is?nen ML, Spector E, Creadon-Swindell G, Br?s-Goldberg AM, Rahikkala E, Moilanen JS, Mahieu V, Matthijs G, Bravo-Alonso I, P?rez-Cerd? C, Ugarte M, Vianey-Saban C, Scharer GH, Van Hove JL
Genetics in medicine : official journal of the American College of Medical Genetics19(1)104-111(2017 Jan)

Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.
Azize NA, Ngah WZ, Othman Z, Md Desa N, Chin CB, Md Yunus Z, Mohan A, Hean TS, Syed Zakaria SZ, Lock-Hock N
Journal of human genetics59(11)593-7(2014 Nov)

Mutations in genes encoding the glycine cleavage system predispose to neural tube defects in mice and humans.
Narisawa A, Komatsuzaki S, Kikuchi A, Niihori T, Aoki Y, Fujiwara K, Tanemura M, Hata A, Suzuki Y, Relton CL, Grinham J, Leung KY, Partridge D, Robinson A, Stone V, Gustavsson P, Stanier P, Copp AJ, Greene ND, Tominaga T, Matsubara Y, Kure S
Human molecular genetics21(7)1496-503(2012 Apr)

A missense mutation (His42Arg) in the T-protein gene from a large Israeli-Arab kindred with nonketotic hyperglycinemia.
Kure S, Mandel H, Rolland MO, Sakata Y, Shinka T, Drugan A, Boneh A, Tada K, Matsubara Y, Narisawa K
Human genetics102(4)430-4(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The position and frequency of the breakpoint for CNVs correlated with intron size and presence of Alu elements. Missense mutations, most often recurring, were the most common type of disease-causing mutation in AMT
Title: The genetic basis of classic nonketotic hyperglycinemia due to mutations in GLDC and AMT.

Data indicate no mutation was found in glycine cleavage system protein-H (GCSH) and suggest that mutations in both glycine decarboxylase (GLDC) and aminomethyltransferase (AMT) are the main cause of glycine encephalopathy in Malaysian population.
Title: Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.

Two unique non-synonymous changes were identified in the AMT gene in patients with neural tube defects.
Title: Mutations in genes encoding the glycine cleavage system predispose to neural tube defects in mice and humans.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia.

The following AMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16475	NM_001164710.2 Latest version!	Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
OHu16563	NM_001164712.2 Latest version!	Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu21803	NM_001164711.2 Latest version!	Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
OHu19608	NM_000481.4 Latest version!	Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu16563	NM_001164712.1	Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu21803	NM_001164711.1	Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
OHu19608	NM_000481.3	Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$237.30 $339.00
OHu16475	NM_001164710.1	Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMT ( NM_001164710.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158182.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16475
Clone ID Related Accession (Same CDS sequence)	NM_001164710.2 , NM_001164710.1
Accession Version	NM_001164710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1, AK296177.1 and GD148179.1. On Jun 2, 2019 this sequence version replaced NM_001164710.1. Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296177.1, SRR1803617.83102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG ACAAGGTCAG GGAGCTTCAG 
AACCAGGGCA GAGATGTGGG CCTGGAGGTG TTGGATAATG CCCTGCTAGC TCTGCAAGGC 
CCCACTGCAG CCCAGGTACT ACAGGCCGGC GTGGCAGATG ACCTGAGGAA ACTGCCCTTC 
ATGACCAGTG CTGTGATGGA GGTGTTTGGC GTGTCTGGCT GCCGCGTGAC CCGCTGTGGC 
TACACAGGAG AGGATGGTGT GGAGATCTCG GTGCCGGTAG CGGGGGCAGT TCACCTGGCA 
ACAGCTATTC TGAAAAACCC AGAGGTGAAG CTGGCAGGGC TGGCAGCCAG GGACAGCCTG 
CGCCTGGAGG CAGGCCTCTG CCTGTATGGG AATGACATTG ATGAACACAC TACACCTGTG 
GAGGGCAGCC TCAGTTGGAC ACTGGGGAAG CGCCGCCGAG CTGCTATGGA CTTCCCTGGA 
GCCAAGGTCA TTGTTCCCCA GCTGAAGGGC AGGGTGCAGC GGAGGCGTGT GGGGTTGATG 
TGTGAGGGGG CCCCCATGCG GGCACACAGT CCCATCCTGA ACATGGAGGG TACCAAGATT 
GGTACTGTGA CTAGTGGCTG CCCCTCCCCC TCTCTGAAGA AGAATGTGGC GATGGGTTAT 
GTGCCCTGCG AGTACAGTCG TCCAGGGACA ATGCTGCTGG TAGAGGTGCG GCGGAAGCAG 
CAGATGGCTG TAGTCAGCAA GATGCCCTTT GTGCCCACAA ACTACTATAC CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158182.1
CDS	24..1103
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_001164710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164710.2

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG ACAAGGTCAG GGAGCTTCAG 
AACCAGGGCA GAGATGTGGG CCTGGAGGTG TTGGATAATG CCCTGCTAGC TCTGCAAGGC 
CCCACTGCAG CCCAGGTACT ACAGGCCGGC GTGGCAGATG ACCTGAGGAA ACTGCCCTTC 
ATGACCAGTG CTGTGATGGA GGTGTTTGGC GTGTCTGGCT GCCGCGTGAC CCGCTGTGGC 
TACACAGGAG AGGATGGTGT GGAGATCTCG GTGCCGGTAG CGGGGGCAGT TCACCTGGCA 
ACAGCTATTC TGAAAAACCC AGAGGTGAAG CTGGCAGGGC TGGCAGCCAG GGACAGCCTG 
CGCCTGGAGG CAGGCCTCTG CCTGTATGGG AATGACATTG ATGAACACAC TACACCTGTG 
GAGGGCAGCC TCAGTTGGAC ACTGGGGAAG CGCCGCCGAG CTGCTATGGA CTTCCCTGGA 
GCCAAGGTCA TTGTTCCCCA GCTGAAGGGC AGGGTGCAGC GGAGGCGTGT GGGGTTGATG 
TGTGAGGGGG CCCCCATGCG GGCACACAGT CCCATCCTGA ACATGGAGGG TACCAAGATT 
GGTACTGTGA CTAGTGGCTG CCCCTCCCCC TCTCTGAAGA AGAATGTGGC GATGGGTTAT 
GTGCCCTGCG AGTACAGTCG TCCAGGGACA ATGCTGCTGG TAGAGGTGCG GCGGAAGCAG 
CAGATGGCTG TAGTCAGCAA GATGCCCTTT GTGCCCACAA ACTACTATAC CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_001164710.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158182.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16475
Clone ID Related Accession (Same CDS sequence)	NM_001164710.2 , NM_001164710.1
Accession Version	NM_001164710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1, AK296177.1 and GD148179.1. Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296177.1, SRR1803617.83102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG ACAAGGTCAG GGAGCTTCAG 
AACCAGGGCA GAGATGTGGG CCTGGAGGTG TTGGATAATG CCCTGCTAGC TCTGCAAGGC 
CCCACTGCAG CCCAGGTACT ACAGGCCGGC GTGGCAGATG ACCTGAGGAA ACTGCCCTTC 
ATGACCAGTG CTGTGATGGA GGTGTTTGGC GTGTCTGGCT GCCGCGTGAC CCGCTGTGGC 
TACACAGGAG AGGATGGTGT GGAGATCTCG GTGCCGGTAG CGGGGGCAGT TCACCTGGCA 
ACAGCTATTC TGAAAAACCC AGAGGTGAAG CTGGCAGGGC TGGCAGCCAG GGACAGCCTG 
CGCCTGGAGG CAGGCCTCTG CCTGTATGGG AATGACATTG ATGAACACAC TACACCTGTG 
GAGGGCAGCC TCAGTTGGAC ACTGGGGAAG CGCCGCCGAG CTGCTATGGA CTTCCCTGGA 
GCCAAGGTCA TTGTTCCCCA GCTGAAGGGC AGGGTGCAGC GGAGGCGTGT GGGGTTGATG 
TGTGAGGGGG CCCCCATGCG GGCACACAGT CCCATCCTGA ACATGGAGGG TACCAAGATT 
GGTACTGTGA CTAGTGGCTG CCCCTCCCCC TCTCTGAAGA AGAATGTGGC GATGGGTTAT 
GTGCCCTGCG AGTACAGTCG TCCAGGGACA ATGCTGCTGG TAGAGGTGCG GCGGAAGCAG 
CAGATGGCTG TAGTCAGCAA GATGCCCTTT GTGCCCACAA ACTACTATAC CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158182.1
CDS	229..1308
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_001164710.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164710.1

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG ACAAGGTCAG GGAGCTTCAG 
AACCAGGGCA GAGATGTGGG CCTGGAGGTG TTGGATAATG CCCTGCTAGC TCTGCAAGGC 
CCCACTGCAG CCCAGGTACT ACAGGCCGGC GTGGCAGATG ACCTGAGGAA ACTGCCCTTC 
ATGACCAGTG CTGTGATGGA GGTGTTTGGC GTGTCTGGCT GCCGCGTGAC CCGCTGTGGC 
TACACAGGAG AGGATGGTGT GGAGATCTCG GTGCCGGTAG CGGGGGCAGT TCACCTGGCA 
ACAGCTATTC TGAAAAACCC AGAGGTGAAG CTGGCAGGGC TGGCAGCCAG GGACAGCCTG 
CGCCTGGAGG CAGGCCTCTG CCTGTATGGG AATGACATTG ATGAACACAC TACACCTGTG 
GAGGGCAGCC TCAGTTGGAC ACTGGGGAAG CGCCGCCGAG CTGCTATGGA CTTCCCTGGA 
GCCAAGGTCA TTGTTCCCCA GCTGAAGGGC AGGGTGCAGC GGAGGCGTGT GGGGTTGATG 
TGTGAGGGGG CCCCCATGCG GGCACACAGT CCCATCCTGA ACATGGAGGG TACCAAGATT 
GGTACTGTGA CTAGTGGCTG CCCCTCCCCC TCTCTGAAGA AGAATGTGGC GATGGGTTAT 
GTGCCCTGCG AGTACAGTCG TCCAGGGACA ATGCTGCTGG TAGAGGTGCG GCGGAAGCAG 
CAGATGGCTG TAGTCAGCAA GATGCCCTTT GTGCCCACAA ACTACTATAC CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_001164712.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158184.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu16563
Clone ID Related Accession (Same CDS sequence)	NM_001164712.1 , NM_001164712.2
Accession Version	NM_001164712.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1 and BC007546.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region that results in a frameshift compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007546.1, SRR1803614.44341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGCTT 
CCTTCAGGAC CCTGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158184.1
CDS	229..1389
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVELPSGPCF

Target ORF information:

RefSeq Version	NM_001164712.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164712.1

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGCTT 
CCTTCAGGAC CCTGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_001164712.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158184.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.

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CloneID	OHu16563
Clone ID Related Accession (Same CDS sequence)	NM_001164712.1 , NM_001164712.2
Accession Version	NM_001164712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1 and BC007546.1. On Jun 2, 2019 this sequence version replaced NM_001164712.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region that results in a frameshift compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1046195.1, BC007546.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGCTT 
CCTTCAGGAC CCTGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158184.1
CDS	24..1184
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVELPSGPCF

Target ORF information:

RefSeq Version	NM_001164712.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164712.2

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGCTT 
CCTTCAGGAC CCTGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_000481.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000472.2 Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.

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CloneID	OHu19608
Clone ID Related Accession (Same CDS sequence)	NM_000481.3 , NM_000481.4
Accession Version	NM_000481.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1, D13811.1 and GD148179.1. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2009 this sequence version replaced NM_000481.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13811.1, SRR1803615.243402.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGGTG 
CGGCGGAAGC AGCAGATGGC TGTAGTCAGC AAGATGCCCT TTGTGCCCAC AAACTACTAT 
ACCCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000472.2
CDS	229..1440
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_000481.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000481.3

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGGTG 
CGGCGGAAGC AGCAGATGGC TGTAGTCAGC AAGATGCCCT TTGTGCCCAC AAACTACTAT 
ACCCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_001164711.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158183.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21803
Clone ID Related Accession (Same CDS sequence)	NM_001164711.1 , NM_001164711.2
Accession Version	NM_001164711.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1, AK293481.1, AC104452.2 and GD148179.1. Transcript Variant: This variant (3) lacks an in-frame exon compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.162656.1, AK293481.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG ACCAAGATAC TTGGTAGTGA CCGGGTGAAG 
CTGATGGAGA GTCTAGTGGT TGGAGACATT GCAGAGCTAA GACCAAACCA GGGGACACTG 
TCGCTGTTTA CCAACGAGGC TGGAGGCATC TTAGATGACT TGATTGTAAC CAATACTTCT 
GAGGGCCACC TGTATGTGGT GTCCAACGCT GGCTGCTGGG AGAAAGATTT GGCCCTCATG 
CAGGACAAGG TCAGGGAGCT TCAGAACCAG GGCAGAGATG TGGGCCTGGA GGTGTTGGAT 
AATGCCCTGC TAGCTCTGCA AGGCCCCACT GCAGCCCAGG TACTACAGGC CGGCGTGGCA 
GATGACCTGA GGAAACTGCC CTTCATGACC AGTGCTGTGA TGGAGGTGTT TGGCGTGTCT 
GGCTGCCGCG TGACCCGCTG TGGCTACACA GGAGAGGATG GTGTGGAGAT CTCGGTGCCG 
GTAGCGGGGG CAGTTCACCT GGCAACAGCT ATTCTGAAAA ACCCAGAGGT GAAGCTGGCA 
GGGCTGGCAG CCAGGGACAG CCTGCGCCTG GAGGCAGGCC TCTGCCTGTA TGGGAATGAC 
ATTGATGAAC ACACTACACC TGTGGAGGGC AGCCTCAGTT GGACACTGGG GAAGCGCCGC 
CGAGCTGCTA TGGACTTCCC TGGAGCCAAG GTCATTGTTC CCCAGCTGAA GGGCAGGGTG 
CAGCGGAGGC GTGTGGGGTT GATGTGTGAG GGGGCCCCCA TGCGGGCACA CAGTCCCATC 
CTGAACATGG AGGGTACCAA GATTGGTACT GTGACTAGTG GCTGCCCCTC CCCCTCTCTG 
AAGAAGAATG TGGCGATGGG TTATGTGCCC TGCGAGTACA GTCGTCCAGG GACAATGCTG 
CTGGTAGAGG TGCGGCGGAA GCAGCAGATG GCTGTAGTCA GCAAGATGCC CTTTGTGCCC 
ACAAACTACT ATACCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158183.1
CDS	229..1272
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_001164711.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164711.1

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG ACCAAGATAC TTGGTAGTGA CCGGGTGAAG 
CTGATGGAGA GTCTAGTGGT TGGAGACATT GCAGAGCTAA GACCAAACCA GGGGACACTG 
TCGCTGTTTA CCAACGAGGC TGGAGGCATC TTAGATGACT TGATTGTAAC CAATACTTCT 
GAGGGCCACC TGTATGTGGT GTCCAACGCT GGCTGCTGGG AGAAAGATTT GGCCCTCATG 
CAGGACAAGG TCAGGGAGCT TCAGAACCAG GGCAGAGATG TGGGCCTGGA GGTGTTGGAT 
AATGCCCTGC TAGCTCTGCA AGGCCCCACT GCAGCCCAGG TACTACAGGC CGGCGTGGCA 
GATGACCTGA GGAAACTGCC CTTCATGACC AGTGCTGTGA TGGAGGTGTT TGGCGTGTCT 
GGCTGCCGCG TGACCCGCTG TGGCTACACA GGAGAGGATG GTGTGGAGAT CTCGGTGCCG 
GTAGCGGGGG CAGTTCACCT GGCAACAGCT ATTCTGAAAA ACCCAGAGGT GAAGCTGGCA 
GGGCTGGCAG CCAGGGACAG CCTGCGCCTG GAGGCAGGCC TCTGCCTGTA TGGGAATGAC 
ATTGATGAAC ACACTACACC TGTGGAGGGC AGCCTCAGTT GGACACTGGG GAAGCGCCGC 
CGAGCTGCTA TGGACTTCCC TGGAGCCAAG GTCATTGTTC CCCAGCTGAA GGGCAGGGTG 
CAGCGGAGGC GTGTGGGGTT GATGTGTGAG GGGGCCCCCA TGCGGGCACA CAGTCCCATC 
CTGAACATGG AGGGTACCAA GATTGGTACT GTGACTAGTG GCTGCCCCTC CCCCTCTCTG 
AAGAAGAATG TGGCGATGGG TTATGTGCCC TGCGAGTACA GTCGTCCAGG GACAATGCTG 
CTGGTAGAGG TGCGGCGGAA GCAGCAGATG GCTGTAGTCA GCAAGATGCC CTTTGTGCCC 
ACAAACTACT ATACCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_001164711.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158183.1 Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21803
Clone ID Related Accession (Same CDS sequence)	NM_001164711.1 , NM_001164711.2
Accession Version	NM_001164711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC387800.1, AK293481.1, AC104452.2 and GD148179.1. On Jun 2, 2019 this sequence version replaced NM_001164711.1. Transcript Variant: This variant (3) lacks an in-frame exon compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.162656.1, AK293481.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG ACCAAGATAC TTGGTAGTGA CCGGGTGAAG 
CTGATGGAGA GTCTAGTGGT TGGAGACATT GCAGAGCTAA GACCAAACCA GGGGACACTG 
TCGCTGTTTA CCAACGAGGC TGGAGGCATC TTAGATGACT TGATTGTAAC CAATACTTCT 
GAGGGCCACC TGTATGTGGT GTCCAACGCT GGCTGCTGGG AGAAAGATTT GGCCCTCATG 
CAGGACAAGG TCAGGGAGCT TCAGAACCAG GGCAGAGATG TGGGCCTGGA GGTGTTGGAT 
AATGCCCTGC TAGCTCTGCA AGGCCCCACT GCAGCCCAGG TACTACAGGC CGGCGTGGCA 
GATGACCTGA GGAAACTGCC CTTCATGACC AGTGCTGTGA TGGAGGTGTT TGGCGTGTCT 
GGCTGCCGCG TGACCCGCTG TGGCTACACA GGAGAGGATG GTGTGGAGAT CTCGGTGCCG 
GTAGCGGGGG CAGTTCACCT GGCAACAGCT ATTCTGAAAA ACCCAGAGGT GAAGCTGGCA 
GGGCTGGCAG CCAGGGACAG CCTGCGCCTG GAGGCAGGCC TCTGCCTGTA TGGGAATGAC 
ATTGATGAAC ACACTACACC TGTGGAGGGC AGCCTCAGTT GGACACTGGG GAAGCGCCGC 
CGAGCTGCTA TGGACTTCCC TGGAGCCAAG GTCATTGTTC CCCAGCTGAA GGGCAGGGTG 
CAGCGGAGGC GTGTGGGGTT GATGTGTGAG GGGGCCCCCA TGCGGGCACA CAGTCCCATC 
CTGAACATGG AGGGTACCAA GATTGGTACT GTGACTAGTG GCTGCCCCTC CCCCTCTCTG 
AAGAAGAATG TGGCGATGGG TTATGTGCCC TGCGAGTACA GTCGTCCAGG GACAATGCTG 
CTGGTAGAGG TGCGGCGGAA GCAGCAGATG GCTGTAGTCA GCAAGATGCC CTTTGTGCCC 
ACAAACTACT ATACCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158183.1
CDS	24..1067
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_001164711.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164711.2

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG ACCAAGATAC TTGGTAGTGA CCGGGTGAAG 
CTGATGGAGA GTCTAGTGGT TGGAGACATT GCAGAGCTAA GACCAAACCA GGGGACACTG 
TCGCTGTTTA CCAACGAGGC TGGAGGCATC TTAGATGACT TGATTGTAAC CAATACTTCT 
GAGGGCCACC TGTATGTGGT GTCCAACGCT GGCTGCTGGG AGAAAGATTT GGCCCTCATG 
CAGGACAAGG TCAGGGAGCT TCAGAACCAG GGCAGAGATG TGGGCCTGGA GGTGTTGGAT 
AATGCCCTGC TAGCTCTGCA AGGCCCCACT GCAGCCCAGG TACTACAGGC CGGCGTGGCA 
GATGACCTGA GGAAACTGCC CTTCATGACC AGTGCTGTGA TGGAGGTGTT TGGCGTGTCT 
GGCTGCCGCG TGACCCGCTG TGGCTACACA GGAGAGGATG GTGTGGAGAT CTCGGTGCCG 
GTAGCGGGGG CAGTTCACCT GGCAACAGCT ATTCTGAAAA ACCCAGAGGT GAAGCTGGCA 
GGGCTGGCAG CCAGGGACAG CCTGCGCCTG GAGGCAGGCC TCTGCCTGTA TGGGAATGAC 
ATTGATGAAC ACACTACACC TGTGGAGGGC AGCCTCAGTT GGACACTGGG GAAGCGCCGC 
CGAGCTGCTA TGGACTTCCC TGGAGCCAAG GTCATTGTTC CCCAGCTGAA GGGCAGGGTG 
CAGCGGAGGC GTGTGGGGTT GATGTGTGAG GGGGCCCCCA TGCGGGCACA CAGTCCCATC 
CTGAACATGG AGGGTACCAA GATTGGTACT GTGACTAGTG GCTGCCCCTC CCCCTCTCTG 
AAGAAGAATG TGGCGATGGG TTATGTGCCC TGCGAGTACA GTCGTCCAGG GACAATGCTG 
CTGGTAGAGG TGCGGCGGAA GCAGCAGATG GCTGTAGTCA GCAAGATGCC CTTTGTGCCC 
ACAAACTACT ATACCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMT ( NM_000481.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000472.2 Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.

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CloneID	OHu19608
Clone ID Related Accession (Same CDS sequence)	NM_000481.3 , NM_000481.4
Accession Version	NM_000481.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aminomethyltransferase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D13811.1 and GD148179.1. On Aug 31, 2019 this sequence version replaced NM_000481.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13811.1, BC094771.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGGTG 
CGGCGGAAGC AGCAGATGGC TGTAGTCAGC AAGATGCCCT TTGTGCCCAC AAACTACTAT 
ACCCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000472.2
CDS	24..1235
Translation	MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK

Target ORF information:

RefSeq Version	NM_000481.4
Organism	Homo sapiens(human)
Definition	Homo sapiens aminomethyltransferase (AMT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000481.4

ORF Insert Sequence:

ATGCAGAGGG CTGTAAGTGT GGTGGCCCGT CTGGGCTTTC GCCTGCAGGC ATTCCCCCCG 
GCCTTGTGTC GTCCACTTAG TTGCGCACAG GAGGTGCTCC GCAGGACACC GCTCTATGAC 
TTCCACCTGG CCCACGGCGG GAAAATGGTG GCGTTTGCGG GTTGGAGTCT GCCAGTGCAG 
TACCGGGACA GTCACACTGA CTCGCACCTG CACACACGCC AGCACTGCTC GCTCTTTGAC 
GTGTCTCATA TGCTGCAGAC CAAGATACTT GGTAGTGACC GGGTGAAGCT GATGGAGAGT 
CTAGTGGTTG GAGACATTGC AGAGCTAAGA CCAAACCAGG GGACACTGTC GCTGTTTACC 
AACGAGGCTG GAGGCATCTT AGATGACTTG ATTGTAACCA ATACTTCTGA GGGCCACCTG 
TATGTGGTGT CCAACGCTGG CTGCTGGGAG AAAGATTTGG CCCTCATGCA GGACAAGGTC 
AGGGAGCTTC AGAACCAGGG CAGAGATGTG GGCCTGGAGG TGTTGGATAA TGCCCTGCTA 
GCTCTGCAAG GCCCCACTGC AGCCCAGGTA CTACAGGCCG GCGTGGCAGA TGACCTGAGG 
AAACTGCCCT TCATGACCAG TGCTGTGATG GAGGTGTTTG GCGTGTCTGG CTGCCGCGTG 
ACCCGCTGTG GCTACACAGG AGAGGATGGT GTGGAGATCT CGGTGCCGGT AGCGGGGGCA 
GTTCACCTGG CAACAGCTAT TCTGAAAAAC CCAGAGGTGA AGCTGGCAGG GCTGGCAGCC 
AGGGACAGCC TGCGCCTGGA GGCAGGCCTC TGCCTGTATG GGAATGACAT TGATGAACAC 
ACTACACCTG TGGAGGGCAG CCTCAGTTGG ACACTGGGGA AGCGCCGCCG AGCTGCTATG 
GACTTCCCTG GAGCCAAGGT CATTGTTCCC CAGCTGAAGG GCAGGGTGCA GCGGAGGCGT 
GTGGGGTTGA TGTGTGAGGG GGCCCCCATG CGGGCACACA GTCCCATCCT GAACATGGAG 
GGTACCAAGA TTGGTACTGT GACTAGTGGC TGCCCCTCCC CCTCTCTGAA GAAGAATGTG 
GCGATGGGTT ATGTGCCCTG CGAGTACAGT CGTCCAGGGA CAATGCTGCT GGTAGAGGTG 
CGGCGGAAGC AGCAGATGGC TGTAGTCAGC AAGATGCCCT TTGTGCCCAC AAACTACTAT 
ACCCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genetic basis of classic nonketotic hyperglycinemia due to mutations in GLDC and AMT.
Genetics in medicine : official journal of the American College of Medical Genetics19(1)104-111(2017 Jan)
Coughlin CR 2nd,Swanson MA,Kronquist K,Acquaviva C,Hutchin T,Rodr?guez-Pombo P,V?is?nen ML,Spector E,Creadon-Swindell G,Br?s-Goldberg AM,Rahikkala E,Moilanen JS,Mahieu V,Matthijs G,Bravo-Alonso I,P?rez-Cerd? C,Ugarte M,Vianey-Saban C,Scharer GH,Van Hove JL

Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.
Journal of human genetics59(11)593-7(2014 Nov)
Azize NA,Ngah WZ,Othman Z,Md Desa N,Chin CB,Md Yunus Z,Mohan A,Hean TS,Syed Zakaria SZ,Lock-Hock N

Mutations in genes encoding the glycine cleavage system predispose to neural tube defects in mice and humans.
Human molecular genetics21(7)1496-503(2012 Apr)
Narisawa A,Komatsuzaki S,Kikuchi A,Niihori T,Aoki Y,Fujiwara K,Tanemura M,Hata A,Suzuki Y,Relton CL,Grinham J,Leung KY,Partridge D,Robinson A,Stone V,Gustavsson P,Stanier P,Copp AJ,Greene ND,Tominaga T,Matsubara Y,Kure S

A missense mutation (His42Arg) in the T-protein gene from a large Israeli-Arab kindred with nonketotic hyperglycinemia.
Human genetics102(4)430-4(1998 Apr)
Kure S,Mandel H,Rolland MO,Sakata Y,Shinka T,Drugan A,Boneh A,Tada K,Matsubara Y,Narisawa K

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AMT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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