GLUD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLUD2
Entrez Gene ID	2747
Full Name	glutamate dehydrogenase 2
Synonyms	GDH2,GLUDP1
General protein information	Preferred Names glutamate dehydrogenase 2 Names glutamate dehydrogenase 2, mitochondrial GDH 2 glutamate dehydrogenase pseudogene 1 testicular secretory protein Li 14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq24
Summary	The protein encoded by this gene is localized to the mitochondrion and acts as a homohexamer to recycle glutamate during neurotransmission. The encoded enzyme catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate. This gene is intronless.[provided by RefSeq, Jan 2010].
Disorder MIM:	300144
Disorder Html:	{Parkinson disease, age of onset, modifier}, 168600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_012084.4	NP_036216.2	glutamate dehydrogenase 2, mitochondrial precursor
NM_012084.3	NP_036216.2	glutamate dehydrogenase 2, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLUD2	NP_036216.2
Pan troglodytes (chimpanzee)	GLUD2	NP_001009004.1
	LOC722094	XP_002805925.1

Gene Ontology
Bibliography

Related articles in PubMed

Glutamate production from ammonia via glutamate dehydrogenase 2 activity supports cancer cell proliferation under glutamine depletion.
Takeuchi Y, Nakayama Y, Fukusaki E, Irino Y
Biochemical and biophysical research communications495(1)761-767(2018 01)

Expression of the human isoform of glutamate dehydrogenase, hGDH2, augments TCA cycle capacity and oxidative metabolism of glutamate during glucose deprivation in astrocytes.
Nissen JD, Lykke K, Bryk J, Stridh MH, Zaganas I, Skytt DM, Schousboe A, Bak LK, Enard W, P??bo S, Waagepetersen HS
Glia65(3)474-488(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This proliferation requires glutamate dehydrogenase 2, which synthesizes glutamate from ammonia and alpha-ketoglutarate and is expressed in MCF7 and T47D cells. Our findings provide insight into how cancer cells survive under glutamine deprivation conditions and thus contribute to elucidating the mechanisms of tumor growth.
Title: Glutamate production from ammonia via glutamate dehydrogenase 2 activity supports cancer cell proliferation under glutamine depletion.

This study demonstrated that hGDH2 expression increases capacity for uptake and oxidative metabolism of glutamate, particularly during increased workload and hypoglycemia.
Title: Expression of the human isoform of glutamate dehydrogenase, hGDH2, augments TCA cycle capacity and oxidative metabolism of glutamate during glucose deprivation in astrocytes.

The expression of GLUD2 was identified in the cellular and subcellular compartments of numerous tissues.
Title: Widening Spectrum of Cellular and Subcellular Expression of Human GLUD1 and GLUD2 Glutamate Dehydrogenases Suggests Novel Functions.

Notably, the introduction of GLUD2 did not affect glutamate levels in mice, consistent with observations in the primates. Instead, the metabolic effects of GLUD2 center on the tricarboxylic acid cycle, suggesting that GLUD2 affects carbon flux during early brain development, possibly supporting lipid biosynthesis.
Title: Mice carrying a human GLUD2 gene recapitulate aspects of human transcriptome and metabolome development.

Study of the expression of the GDH1/2 in human steroidogenic organs revealed that, while GDH2 was expressed specifically in steroid-synthesizing cells, GDH1 was expressed both in the cells that produce steroids and in those that lack endocrine function.
Title: Expression of human GLUD1 and GLUD2 glutamate dehydrogenases in steroid producing tissues.

The following GLUD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLUD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18663	NM_012084.4 Latest version!	Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu18663	NM_012084.3	Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLUD2 ( NM_012084.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036216.2 Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.

Price & Availability↑

CloneID	OHu18663
Clone ID Related Accession (Same CDS sequence)	NM_012084.4 , NM_012084.3
Accession Version	NM_012084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	glutamate dehydrogenase 2, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC050732.2 and AC006144.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_012084.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: HM005554.1, BC050732.2 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000328078.3/ ENSP00000327589.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCGCT ACCTGGCCAA AGCGCTGCTG CCGTCCCGGG CCGGGCCCGC TGCCCTGGGC 
TCCGCGGCCA ACCACTCGGC CGCGTTGCTG GGCCGGGGCC GCGGACAGCC CGCCGCCGCC 
TCGCAGCCGG GGCTCGCATT GGCCGCCCGG CGCCACTACA GCGAGTTGGT GGCCGACCGC 
GAGGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGATCGCGG CGCCAGCATC 
GTGGAGGACA AGTTGGTGAA GGACCTGAGG ACCCAGGAAA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGCG GATCATCAAG CCCTGCAACC ATGTGCTGAG TCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGATGTGAG TGTAGATGAA 
GTAAAAGCTT TGGCTTCTCT GATGACATAC AAGTGTGCAG TGGTTGATGT GCCGTTTGGG 
GGTGCTAAAG CTGGTGTTAA GATCAATCCC AAGAACTATA CCGAAAATGA ATTGGAAAAG 
ATCACAAGGA GGTTCACCAT GGAGCTAGCA AAGAAGGGCT TTATTGGTCC TGGCGTTGAT 
GTGCCTGCTC CAGACATGAA CACAGGTGAG CGGGAGATGT CCTGGATTGC TGATACCTAT 
GCCAGCACCA TAGGGCACTA TGATATTAAT GCACACGCCT GTGTTACTGG TAAACCCATC 
AGCCAAGGGG GAATCCATGG ACGCATCTCT GCTACTGGCC GTGGTGTCTT CCATGGGATT 
GAAAACTTCA TCAATGAAGC TTCTTACATG AGCATTTTAG GAATGACACC AGGGTTTAGA 
GATAAAACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TACACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TATTGCTGTT GGTGAGTCTG ATGGGAGTAT ATGGAATCCA 
GATGGTATTG ACCCAAAGGA ACTGGAAGAC TTCAAATTGC AACATGGGTC CATTCTGGGC 
TTCCCCAAGG CAAAGCCCTA TGAAGGAAGC ATCTTGGAGG TCGACTGTGA CATACTGATC 
CCAGCTGCCA CTGAGAAGCA GTTGACCAAA TCCAACGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACAACT CCAGAAGCTG ATAAGATCTT CCTGGAGAGA 
AACATTTTGG TTATTCCAGA TCTCTACTTG AATGCTGGAG GAGTGACAGT ATCTTACTTT 
GAGTGGCTGA AGAATCTAAA TCATGTCAGC TATGGCCGTT TGACCTTCAA ATATGAAAGG 
GATTCTAACT ACCACTTGCT CCTGTCTGTT CAAGAGAGTT TAGAAAGAAA ATTTGGAAAG 
CATGGTGGAA CTATTCCCAT TGTACCCACG GCAGAGTTCC AAGACAGTAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGCCTTG GCATACACAA TGGAGCGTTC TGCCAGGCAA 
ATTATGCACA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCCATTG AAAAAGTCTT CAAAGTGTAC AGTGAAGCTG GTGTGACCTT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036216.2
CDS	76..1752
Translation	MYRYLAKALLPSRAGPAALGSAANHSAALLGRGRGQPAAASQPGLALAARRHYSELVADREDDPNFFKMVEGFFDRGASIVEDKLVKDLRTQESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTENELEKITRRFTMELAKKGFIGPGVDVPAPDMNTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFRDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEVDCDILIPAATEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNILVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLLSVQESLERKFGKHGGTIPIVPTAEFQDSISGASEKDIVHSALAYTMERSARQIMHTAMKYNLGLDLRTAAYVNAIEKVFKVYSEAGVTFT

Target ORF information:

RefSeq Version	NM_012084.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012084.4

ORF Insert Sequence:

ATGTACCGCT ACCTGGCCAA AGCGCTGCTG CCGTCCCGGG CCGGGCCCGC TGCCCTGGGC 
TCCGCGGCCA ACCACTCGGC CGCGTTGCTG GGCCGGGGCC GCGGACAGCC CGCCGCCGCC 
TCGCAGCCGG GGCTCGCATT GGCCGCCCGG CGCCACTACA GCGAGTTGGT GGCCGACCGC 
GAGGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGATCGCGG CGCCAGCATC 
GTGGAGGACA AGTTGGTGAA GGACCTGAGG ACCCAGGAAA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGCG GATCATCAAG CCCTGCAACC ATGTGCTGAG TCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGATGTGAG TGTAGATGAA 
GTAAAAGCTT TGGCTTCTCT GATGACATAC AAGTGTGCAG TGGTTGATGT GCCGTTTGGG 
GGTGCTAAAG CTGGTGTTAA GATCAATCCC AAGAACTATA CCGAAAATGA ATTGGAAAAG 
ATCACAAGGA GGTTCACCAT GGAGCTAGCA AAGAAGGGCT TTATTGGTCC TGGCGTTGAT 
GTGCCTGCTC CAGACATGAA CACAGGTGAG CGGGAGATGT CCTGGATTGC TGATACCTAT 
GCCAGCACCA TAGGGCACTA TGATATTAAT GCACACGCCT GTGTTACTGG TAAACCCATC 
AGCCAAGGGG GAATCCATGG ACGCATCTCT GCTACTGGCC GTGGTGTCTT CCATGGGATT 
GAAAACTTCA TCAATGAAGC TTCTTACATG AGCATTTTAG GAATGACACC AGGGTTTAGA 
GATAAAACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TACACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TATTGCTGTT GGTGAGTCTG ATGGGAGTAT ATGGAATCCA 
GATGGTATTG ACCCAAAGGA ACTGGAAGAC TTCAAATTGC AACATGGGTC CATTCTGGGC 
TTCCCCAAGG CAAAGCCCTA TGAAGGAAGC ATCTTGGAGG TCGACTGTGA CATACTGATC 
CCAGCTGCCA CTGAGAAGCA GTTGACCAAA TCCAACGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACAACT CCAGAAGCTG ATAAGATCTT CCTGGAGAGA 
AACATTTTGG TTATTCCAGA TCTCTACTTG AATGCTGGAG GAGTGACAGT ATCTTACTTT 
GAGTGGCTGA AGAATCTAAA TCATGTCAGC TATGGCCGTT TGACCTTCAA ATATGAAAGG 
GATTCTAACT ACCACTTGCT CCTGTCTGTT CAAGAGAGTT TAGAAAGAAA ATTTGGAAAG 
CATGGTGGAA CTATTCCCAT TGTACCCACG GCAGAGTTCC AAGACAGTAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGCCTTG GCATACACAA TGGAGCGTTC TGCCAGGCAA 
ATTATGCACA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCCATTG AAAAAGTCTT CAAAGTGTAC AGTGAAGCTG GTGTGACCTT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLUD2 ( NM_012084.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036216.2 Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.

Price & Availability↑

CloneID	OHu18663
Clone ID Related Accession (Same CDS sequence)	NM_012084.4 , NM_012084.3
Accession Version	NM_012084.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	glutamate dehydrogenase 2, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC050732.2. This sequence is a reference standard in the RefSeqGene project. On Aug 26, 2003 this sequence version replaced NM_012084.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC050732.2 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCGCT ACCTGGCCAA AGCGCTGCTG CCGTCCCGGG CCGGGCCCGC TGCCCTGGGC 
TCCGCGGCCA ACCACTCGGC CGCGTTGCTG GGCCGGGGCC GCGGACAGCC CGCCGCCGCC 
TCGCAGCCGG GGCTCGCATT GGCCGCCCGG CGCCACTACA GCGAGTTGGT GGCCGACCGC 
GAGGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGATCGCGG CGCCAGCATC 
GTGGAGGACA AGTTGGTGAA GGACCTGAGG ACCCAGGAAA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGCG GATCATCAAG CCCTGCAACC ATGTGCTGAG TCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGATGTGAG TGTAGATGAA 
GTAAAAGCTT TGGCTTCTCT GATGACATAC AAGTGTGCAG TGGTTGATGT GCCGTTTGGG 
GGTGCTAAAG CTGGTGTTAA GATCAATCCC AAGAACTATA CCGAAAATGA ATTGGAAAAG 
ATCACAAGGA GGTTCACCAT GGAGCTAGCA AAGAAGGGCT TTATTGGTCC TGGCGTTGAT 
GTGCCTGCTC CAGACATGAA CACAGGTGAG CGGGAGATGT CCTGGATTGC TGATACCTAT 
GCCAGCACCA TAGGGCACTA TGATATTAAT GCACACGCCT GTGTTACTGG TAAACCCATC 
AGCCAAGGGG GAATCCATGG ACGCATCTCT GCTACTGGCC GTGGTGTCTT CCATGGGATT 
GAAAACTTCA TCAATGAAGC TTCTTACATG AGCATTTTAG GAATGACACC AGGGTTTAGA 
GATAAAACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TACACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TATTGCTGTT GGTGAGTCTG ATGGGAGTAT ATGGAATCCA 
GATGGTATTG ACCCAAAGGA ACTGGAAGAC TTCAAATTGC AACATGGGTC CATTCTGGGC 
TTCCCCAAGG CAAAGCCCTA TGAAGGAAGC ATCTTGGAGG TCGACTGTGA CATACTGATC 
CCAGCTGCCA CTGAGAAGCA GTTGACCAAA TCCAACGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACAACT CCAGAAGCTG ATAAGATCTT CCTGGAGAGA 
AACATTTTGG TTATTCCAGA TCTCTACTTG AATGCTGGAG GAGTGACAGT ATCTTACTTT 
GAGTGGCTGA AGAATCTAAA TCATGTCAGC TATGGCCGTT TGACCTTCAA ATATGAAAGG 
GATTCTAACT ACCACTTGCT CCTGTCTGTT CAAGAGAGTT TAGAAAGAAA ATTTGGAAAG 
CATGGTGGAA CTATTCCCAT TGTACCCACG GCAGAGTTCC AAGACAGTAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGCCTTG GCATACACAA TGGAGCGTTC TGCCAGGCAA 
ATTATGCACA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCCATTG AAAAAGTCTT CAAAGTGTAC AGTGAAGCTG GTGTGACCTT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036216.2
CDS	78..1754
Translation	MYRYLAKALLPSRAGPAALGSAANHSAALLGRGRGQPAAASQPGLALAARRHYSELVADREDDPNFFKMVEGFFDRGASIVEDKLVKDLRTQESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTENELEKITRRFTMELAKKGFIGPGVDVPAPDMNTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFRDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEVDCDILIPAATEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNILVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLLSVQESLERKFGKHGGTIPIVPTAEFQDSISGASEKDIVHSALAYTMERSARQIMHTAMKYNLGLDLRTAAYVNAIEKVFKVYSEAGVTFT

Target ORF information:

RefSeq Version	NM_012084.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate dehydrogenase 2 (GLUD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012084.3

ORF Insert Sequence:

ATGTACCGCT ACCTGGCCAA AGCGCTGCTG CCGTCCCGGG CCGGGCCCGC TGCCCTGGGC 
TCCGCGGCCA ACCACTCGGC CGCGTTGCTG GGCCGGGGCC GCGGACAGCC CGCCGCCGCC 
TCGCAGCCGG GGCTCGCATT GGCCGCCCGG CGCCACTACA GCGAGTTGGT GGCCGACCGC 
GAGGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGATCGCGG CGCCAGCATC 
GTGGAGGACA AGTTGGTGAA GGACCTGAGG ACCCAGGAAA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGCG GATCATCAAG CCCTGCAACC ATGTGCTGAG TCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGATGTGAG TGTAGATGAA 
GTAAAAGCTT TGGCTTCTCT GATGACATAC AAGTGTGCAG TGGTTGATGT GCCGTTTGGG 
GGTGCTAAAG CTGGTGTTAA GATCAATCCC AAGAACTATA CCGAAAATGA ATTGGAAAAG 
ATCACAAGGA GGTTCACCAT GGAGCTAGCA AAGAAGGGCT TTATTGGTCC TGGCGTTGAT 
GTGCCTGCTC CAGACATGAA CACAGGTGAG CGGGAGATGT CCTGGATTGC TGATACCTAT 
GCCAGCACCA TAGGGCACTA TGATATTAAT GCACACGCCT GTGTTACTGG TAAACCCATC 
AGCCAAGGGG GAATCCATGG ACGCATCTCT GCTACTGGCC GTGGTGTCTT CCATGGGATT 
GAAAACTTCA TCAATGAAGC TTCTTACATG AGCATTTTAG GAATGACACC AGGGTTTAGA 
GATAAAACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TACACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TATTGCTGTT GGTGAGTCTG ATGGGAGTAT ATGGAATCCA 
GATGGTATTG ACCCAAAGGA ACTGGAAGAC TTCAAATTGC AACATGGGTC CATTCTGGGC 
TTCCCCAAGG CAAAGCCCTA TGAAGGAAGC ATCTTGGAGG TCGACTGTGA CATACTGATC 
CCAGCTGCCA CTGAGAAGCA GTTGACCAAA TCCAACGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACAACT CCAGAAGCTG ATAAGATCTT CCTGGAGAGA 
AACATTTTGG TTATTCCAGA TCTCTACTTG AATGCTGGAG GAGTGACAGT ATCTTACTTT 
GAGTGGCTGA AGAATCTAAA TCATGTCAGC TATGGCCGTT TGACCTTCAA ATATGAAAGG 
GATTCTAACT ACCACTTGCT CCTGTCTGTT CAAGAGAGTT TAGAAAGAAA ATTTGGAAAG 
CATGGTGGAA CTATTCCCAT TGTACCCACG GCAGAGTTCC AAGACAGTAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGCCTTG GCATACACAA TGGAGCGTTC TGCCAGGCAA 
ATTATGCACA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCCATTG AAAAAGTCTT CAAAGTGTAC AGTGAAGCTG GTGTGACCTT CACATAG