GLRA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLRA1
Entrez Gene ID	2741
Full Name	glycine receptor alpha 1
Synonyms	HKPX1,STHE
General protein information	Preferred Names glycine receptor alpha 1 Names glycine receptor subunit alpha-1 glycine receptor 48 kDa subunit glycine receptor strychnine-binding subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q33.1
Summary	The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015].
Disorder MIM:	138491
Disorder Html:	Hyperekplexia, hereditary 1, autosomal dominant or recessive, 149400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001292000.1	NP_001278929.1	glycine receptor subunit alpha-1 isoform 3
NM_001146040.1	NP_001139512.1	glycine receptor subunit alpha-1 isoform 1 precursor
NM_001292000.2	NP_001278929.1	glycine receptor subunit alpha-1 isoform 3
NM_001146040.2	NP_001139512.1	glycine receptor subunit alpha-1 isoform 1 precursor
NM_000171.4	NP_000162.2	glycine receptor subunit alpha-1 isoform 2 precursor
NM_000171.3	NP_000162.2	glycine receptor subunit alpha-1 isoform 2 precursor
XM_017009349.1	XP_016864838.1	glycine receptor subunit alpha-1 isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	avr-15	NP_001024077.1
Xenopus tropicalis (tropical clawed frog)	glra1	XP_004912784.1
	GLRA1	XP_002804635.1
Drosophila melanogaster (fruit fly)	GluClalpha	NP_001262740.1
Caenorhabditis elegans (roundworm)	avr-15	NP_001024076.1
Homo sapiens (human)	GLRA1	NP_001139512.1
Pan troglodytes (chimpanzee)	GLRA1	XP_001169235.1
Bos taurus (cattle)	GLRA1	NP_776746.1
Mus musculus (house mouse)	Glra1	NP_065238.2
Rattus norvegicus (Norway rat)	Glra1	NP_037265.1
Caenorhabditis elegans (roundworm)	avr-14	NP_001020963.1
Caenorhabditis elegans (roundworm)	glc-3	NP_504441.1
Gallus gallus (chicken)	GLRA1	XP_425210.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001434	XP_003435829.1
Caenorhabditis elegans (roundworm)	glc-1	NP_507090.1
Canis lupus familiaris (dog)	GLRA1	XP_003434572.1
Danio rerio (zebrafish)	glra1	NP_571477.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations affecting glycinergic neurotransmission in hyperekplexia increase pain sensitivity.
Vuilleumier PH, Fritsche R, Schliessbach J, Schmitt B, Arendt-Nielsen L, Zeilhofer HU, Curatolo M
Brain : a journal of neurology141(1)63-71(2018 Jan)

Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers.
Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG
Scientific reports7(1)10899(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The E103K startle mutation reduces the sensitivity of glycine receptor alpha 1 to both glycine and sarcosine and impairs channel gating.
Title: The Startle Disease Mutation E103K Impairs Activation of Human Homomeric ?1 Glycine Receptors by Disrupting an Intersubunit Salt Bridge across the Agonist Binding Site.

Results support the notion that the efficacies of ligands for the alpha1 GlyR are determined by conformational changes that occur within and near the ligand-binding domain: disruption of the D97-R119 interaction may be an important element in receptor activation and that agonists may break this putative intersubunit bond in order to destabilize the initial shut state and facilitate transitions towards the open state.
Title: Disruption of a putative intersubunit electrostatic bond enhances agonist efficacy at the human ?1 glycine receptor.

gain-of-function GLRA1 mutations also cause hyperekplexia, although the mechanism is unknown. Here we identify two new gain-of-function mutations (I43F and W170S) and characterize these along with known gain-of-function mutations (Q226E, V280M, and R414H) to identify how they cause hyperekplexia.
Title: Investigating the Mechanism by Which Gain-of-function Mutations to the ?1 Glycine Receptor Cause Hyperekplexia.

the TM3-4 loop length is critical for glycine receptor alpha1 desensitization and a direct neighborhood of both basic stretches changes receptor properties from non-desensitizing to desensitizing.
Title: Length of the TM3-4 loop of the glycine receptor modulates receptor desensitization.

GLRA1 and GLRB mutations are responsible for abnormal startled reactions in humans. (Review)
Title: [GLYCINE RECEPTOR: MOLECULAR ORGANIZATION AND PATHOLOGY].

The following GLRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26270	NM_001146040.2 Latest version!	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26374	NM_000171.4 Latest version!	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu24622	NM_001292000.2 Latest version!	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu95827	XM_017009349.1 Latest version!	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu24622	NM_001292000.1	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu26270	NM_001146040.1	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26374	NM_000171.3	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLRA1 ( NM_001146040.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139512.1 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26270
Clone ID Related Accession (Same CDS sequence)	NM_001146040.1 , NM_001146040.2
Accession Version	NM_001146040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, BC114947.1 and AK226046.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114947.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC 
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC 
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC 
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC 
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG 
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139512.1
CDS	288..1661
Translation	MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_001146040.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146040.1

ORF Insert Sequence:

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC 
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC 
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC 
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC 
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG 
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( NM_001146040.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139512.1 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26270
Clone ID Related Accession (Same CDS sequence)	NM_001146040.1 , NM_001146040.2
Accession Version	NM_001146040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010312.5, X52009.1, BC114947.1 and AK226046.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001146040.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114947.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC 
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC 
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC 
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC 
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG 
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139512.1
CDS	303..1676
Translation	MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_001146040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146040.2

ORF Insert Sequence:

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC 
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC 
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC 
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC 
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG 
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( NM_000171.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000162.2 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26374
Clone ID Related Accession (Same CDS sequence)	NM_000171.4 , NM_000171.3
Accession Version	NM_000171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010312.5, X52009.1, AK312702.1, BC074980.2 and AK226046.1. On Jun 2, 2019 this sequence version replaced NM_000171.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312702.1, BC074980.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC 
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC 
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC 
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC 
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT 
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000162.2
CDS	303..1652
Translation	MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_000171.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000171.4

ORF Insert Sequence:

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC 
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC 
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC 
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC 
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT 
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( NM_000171.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000162.2 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26374
Clone ID Related Accession (Same CDS sequence)	NM_000171.4 , NM_000171.3
Accession Version	NM_000171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, AK312702.1, BC074980.2 and AK226046.1. On Mar 26, 2009 this sequence version replaced NM_000171.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52009.1, AK312702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC 
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC 
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC 
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC 
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT 
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000162.2
CDS	288..1637
Translation	MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_000171.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000171.3

ORF Insert Sequence:

ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT 
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT 
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT 
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC 
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC 
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC 
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC 
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT 
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA 
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG 
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC 
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG 
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT 
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA 
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC 
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG 
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC 
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC 
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC 
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC 
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT 
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( NM_001292000.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278929.1 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.

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CloneID	OHu24622
Clone ID Related Accession (Same CDS sequence)	NM_001292000.1 , NM_001292000.2
Accession Version	NM_001292000.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC114967.1, AK308539.1 and AK226046.1. On May 15, 2014 this sequence version replaced XM_005268411.1. Transcript Variant: This variant (3) lacks an internal exon in the 5' region, which results in translation initiation at a downstream AUG start codon, and contains an alternate splice site at the 5' end of the last exon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226046.1, BC114967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA 
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC 
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA 
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA 
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC 
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG 
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC 
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC 
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA 
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC 
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC 
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC 
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC 
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG 
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG 
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC 
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA 
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278929.1
CDS	409..1509
Translation	MDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_001292000.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292000.1

ORF Insert Sequence:

ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA 
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC 
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA 
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA 
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC 
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG 
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC 
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC 
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA 
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC 
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC 
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC 
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC 
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG 
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG 
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC 
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA 
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( NM_001292000.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278929.1 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24622
Clone ID Related Accession (Same CDS sequence)	NM_001292000.1 , NM_001292000.2
Accession Version	NM_001292000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010312.5, BC114967.1, AK308539.1 and AK226046.1. On Jun 2, 2019 this sequence version replaced NM_001292000.1. Transcript Variant: This variant (3) lacks an internal exon in the 5' region, which results in translation initiation at a downstream AUG start codon, and contains an alternate splice site at the 5' end of the last exon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226046.1, BC114967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA 
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC 
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA 
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA 
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC 
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG 
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC 
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC 
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA 
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC 
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC 
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC 
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC 
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG 
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG 
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC 
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA 
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278929.1
CDS	424..1524
Translation	MDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	NM_001292000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292000.2

ORF Insert Sequence:

ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA 
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC 
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA 
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA 
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC 
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG 
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC 
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC 
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA 
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC 
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC 
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC 
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC 
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG 
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG 
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC 
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA 
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLRA1 ( XM_017009349.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864838.1 Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95827
Clone ID Related Accession (Same CDS sequence)	XM_017009349.1
Accession Version	XM_017009349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glycine receptor subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGGCATTC AACCAAACTT AGAGCCCACC TTAATAAAAG GCAACCTGTA CCAGCATGAG 
AAAGAGCCAT CATTAACCTT TTGCTCTCCT CTCTTCCTCC TTAGCCTTGC TGCTTCTAAG 
GAGGCTGAAG CTGCTCGCTC CGCACCCAAG CCTATGTCAC CCTCGGATTT CCTGGATAAG 
CTAATGGGGA GAACCTCCGG ATATGATGCC AGGATCAGGC CCAATTTTAA AGGTCCCCCA 
GTGAACGTGA GCTGCAACAT TTTCATCAAC AGCTTTGGTT CCATTGCTGA GACAACCATG 
GACTATAGGG TCAACATCTT CCTGCGGCAG CAATGGAACG ACCCCCGCCT GGCCTATAAT 
GAATACCCTG ACGACTCTCT GGACCTGGAC CCATCCATGC TGGACTCCAT CTGGAAACCT 
GACCTGTTCT TTGCCAACGA GAAGGGGGCC CACTTCCATG AGATCACCAC AGACAACAAA 
TTGCTAAGGA TCTCCCGGAA TGGGAATGTC CTCTACAGCA TCAGAATCAC CCTGACACTG 
GCCTGCCCCA TGGACTTGAA GAATTTCCCC ATGGATGTCC AGACATGTAT CATGCAACTG 
GAAAGCTTTG GATATACGAT GAATGACCTC ATCTTTGAGT GGCAGGAACA GGGAGCCGTG 
CAGGTAGCAG ATGGACTAAC TCTGCCCCAG TTTATCTTGA AGGAAGAGAA GGACTTGAGA 
TACTGCACCA AGCACTACAA CACAGGTAAA TTCACCTGCA TTGAGGCCCG GTTCCACCTG 
GAGCGGCAGA TGGGTTACTA CCTGATTCAG ATGTATATTC CCAGCCTGCT CATTGTCATC 
CTCTCATGGA TCTCCTTCTG GATCAACATG GATGCTGCAC CTGCTCGTGT GGGCCTAGGC 
ATCACCACTG TGCTCACCAT GACCACCCAG AGCTCCGGCT CTCGAGCATC TCTGCCCAAG 
GTGTCCTATG TGAAAGCCAT TGACATTTGG ATGGCAGTTT GCCTGCTCTT TGTGTTCTCA 
GCCCTATTAG AATATGCTGC CGTTAACTTT GTGTCTCGGC AACATAAGGA GCTGCTCCGA 
TTCAGGAGGA AGCGGAGACA TCACAAGGAG GATGAAGCTG GAGAAGGCCG CTTTAACTTC 
TCTGCCTATG GGATGGGCCC AGCCTGTCTA CAGGCCAAGG ATGGCATCTC AGTCAAGGGC 
GCCAACAACA GTAACACCAC CAACCCCCCT CCTGCACCAT CTAAGTCCCC AGAGGAGATG 
CGAAAACTCT TCATCCAGAG GGCCAAGAAG ATCGACAAAA TATCCCGCAT TGGCTTCCCC 
ATGGCCTTCC TCATTTTCAA CATGTTCTAC TGGATCATCT ACAAGATTGT CCGTAGAGAG 
GACGTCCACA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864838.1
CDS	1..1398
Translation	MGIQPNLEPTLIKGNLYQHEKEPSLTFCSPLFLLSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ

Target ORF information:

RefSeq Version	XM_017009349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009349.1

ORF Insert Sequence:

ATGGGCATTC AACCAAACTT AGAGCCCACC TTAATAAAAG GCAACCTGTA CCAGCATGAG 
AAAGAGCCAT CATTAACCTT TTGCTCTCCT CTCTTCCTCC TTAGCCTTGC TGCTTCTAAG 
GAGGCTGAAG CTGCTCGCTC CGCACCCAAG CCTATGTCAC CCTCGGATTT CCTGGATAAG 
CTAATGGGGA GAACCTCCGG ATATGATGCC AGGATCAGGC CCAATTTTAA AGGTCCCCCA 
GTGAACGTGA GCTGCAACAT TTTCATCAAC AGCTTTGGTT CCATTGCTGA GACAACCATG 
GACTATAGGG TCAACATCTT CCTGCGGCAG CAATGGAACG ACCCCCGCCT GGCCTATAAT 
GAATACCCTG ACGACTCTCT GGACCTGGAC CCATCCATGC TGGACTCCAT CTGGAAACCT 
GACCTGTTCT TTGCCAACGA GAAGGGGGCC CACTTCCATG AGATCACCAC AGACAACAAA 
TTGCTAAGGA TCTCCCGGAA TGGGAATGTC CTCTACAGCA TCAGAATCAC CCTGACACTG 
GCCTGCCCCA TGGACTTGAA GAATTTCCCC ATGGATGTCC AGACATGTAT CATGCAACTG 
GAAAGCTTTG GATATACGAT GAATGACCTC ATCTTTGAGT GGCAGGAACA GGGAGCCGTG 
CAGGTAGCAG ATGGACTAAC TCTGCCCCAG TTTATCTTGA AGGAAGAGAA GGACTTGAGA 
TACTGCACCA AGCACTACAA CACAGGTAAA TTCACCTGCA TTGAGGCCCG GTTCCACCTG 
GAGCGGCAGA TGGGTTACTA CCTGATTCAG ATGTATATTC CCAGCCTGCT CATTGTCATC 
CTCTCATGGA TCTCCTTCTG GATCAACATG GATGCTGCAC CTGCTCGTGT GGGCCTAGGC 
ATCACCACTG TGCTCACCAT GACCACCCAG AGCTCCGGCT CTCGAGCATC TCTGCCCAAG 
GTGTCCTATG TGAAAGCCAT TGACATTTGG ATGGCAGTTT GCCTGCTCTT TGTGTTCTCA 
GCCCTATTAG AATATGCTGC CGTTAACTTT GTGTCTCGGC AACATAAGGA GCTGCTCCGA 
TTCAGGAGGA AGCGGAGACA TCACAAGGAG GATGAAGCTG GAGAAGGCCG CTTTAACTTC 
TCTGCCTATG GGATGGGCCC AGCCTGTCTA CAGGCCAAGG ATGGCATCTC AGTCAAGGGC 
GCCAACAACA GTAACACCAC CAACCCCCCT CCTGCACCAT CTAAGTCCCC AGAGGAGATG 
CGAAAACTCT TCATCCAGAG GGCCAAGAAG ATCGACAAAA TATCCCGCAT TGGCTTCCCC 
ATGGCCTTCC TCATTTTCAA CATGTTCTAC TGGATCATCT ACAAGATTGT CCGTAGAGAG 
GACGTCCACA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations affecting glycinergic neurotransmission in hyperekplexia increase pain sensitivity.
Brain : a journal of neurology141(1)63-71(2018 Jan)
Vuilleumier PH,Fritsche R,Schliessbach J,Schmitt B,Arendt-Nielsen L,Zeilhofer HU,Curatolo M

Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers.
Scientific reports7(1)10899(2017 Sep)
Patrizio A,Renner M,Pizzarelli R,Triller A,Specht CG

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GLRA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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