TIMM8B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMM8B
Entrez Gene ID	26521
Full Name	translocase of inner mitochondrial membrane 8 homolog B
Synonyms	DDP2,TIM8B
General protein information	Preferred Names translocase of inner mitochondrial membrane 8 homolog B Names mitochondrial import inner membrane translocase subunit Tim8 B DDP-like protein deafness dystonia protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.1
Summary	This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.[provided by RefSeq, Aug 2009].
Disorder MIM:	606659

mRNA and Protein(s)

mRNA	Protein	Name
NM_012459.4	NP_036591.3	mitochondrial import inner membrane translocase subunit Tim8 B
NM_012459.3	NP_036591.3	mitochondrial import inner membrane translocase subunit Tim8 B
NM_012459.2	NP_036591.2	mitochondrial import inner membrane translocase subunit Tim8 B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TIMM8B	XP_854099.3
Rattus norvegicus (Norway rat)	Timm8b	NP_071986.1
Xenopus tropicalis (tropical clawed frog)	timm8b	NP_001017099.1
Drosophila melanogaster (fruit fly)	Tim8	NP_572713.1
Pan troglodytes (chimpanzee)	TIMM8B	XP_001146364.2
Danio rerio (zebrafish)	timm8b	NP_001122251.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010606	XP_314568.3
Bos taurus (cattle)	TIMM8B	NP_001032718.1
Homo sapiens (human)	TIMM8B	NP_036591.2
	TIMM8B	XP_001107083.2
Mus musculus (house mouse)	Timm8b	NP_038925.1

Gene Ontology
Bibliography

Related articles in PubMed

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller.
Webb CT, Gorman MA, Lazarou M, Ryan MT, Gulbis JM
Molecular cell21(1)123-33(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following TIMM8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMM8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22424	NM_012459.2 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107542	NM_012459.4 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu107542	NM_012459.3	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMM8B ( NM_012459.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036591.2 Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22424
Clone ID Related Accession (Same CDS sequence)	NM_012459.2
Accession Version	NM_012459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit Tim8 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002007.4, BC105986.1 and CF541334.1. This sequence is a reference standard in the RefSeqGene project. On Aug 28, 2009 this sequence version replaced NM_012459.1. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.379001.1, SRR1803616.22042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCGCAAAC ACAGCTGTCG GAAGGTGGCG AGCCTGAGGC GAACAATGGC GGAGCTGGGC 
GAAGCCGATG AAGCGGAGTT GCAGCGCCTG GTGGCCGCCG AGCAGCAGAA GGCGCAGTTT 
ACTGCACAGG TGCATCACTT CATGGAGTTA TGTTGGGATA AATGTGTGGA GAAGCCAGGG 
AATCGCCTAG ACTCTCGCAC TGAAAATTGT CTCTCCAGCT GTGTAGACCG CTTCATTGAC 
ACCACTCTTG CCATCACCAG TCGGTTTGCC CAGATTGTAC AGAAAGGAGG GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036591.2
CDS	31..327
Translation	MRKHSCRKVASLRRTMAELGEADEAELQRLVAAEQQKAQFTAQVHHFMELCWDKCVEKPGNRLDSRTENCLSSCVDRFIDTTLAITSRFAQIVQKGGQ

Target ORF information:

RefSeq Version	NM_012459.2
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012459.2

ORF Insert Sequence:

ATGCGCAAAC ACAGCTGTCG GAAGGTGGCG AGCCTGAGGC GAACAATGGC GGAGCTGGGC 
GAAGCCGATG AAGCGGAGTT GCAGCGCCTG GTGGCCGCCG AGCAGCAGAA GGCGCAGTTT 
ACTGCACAGG TGCATCACTT CATGGAGTTA TGTTGGGATA AATGTGTGGA GAAGCCAGGG 
AATCGCCTAG ACTCTCGCAC TGAAAATTGT CTCTCCAGCT GTGTAGACCG CTTCATTGAC 
ACCACTCTTG CCATCACCAG TCGGTTTGCC CAGATTGTAC AGAAAGGAGG GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM8B ( NM_012459.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036591.3 Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107542
Clone ID Related Accession (Same CDS sequence)	NM_012459.4 , NM_012459.3
Accession Version	NM_012459.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit Tim8 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002007.4. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_012459.3. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is supported by conservation data. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.806844.1, SRR1803613.60468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000504148.3/ ENSP00000422122.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGC TGGGCGAAGC CGATGAAGCG GAGTTGCAGC GCCTGGTGGC CGCCGAGCAG 
CAGAAGGCGC AGTTTACTGC ACAGGTGCAT CACTTCATGG AGTTATGTTG GGATAAATGT 
GTGGAGAAGC CAGGGAATCG CCTAGACTCT CGCACTGAAA ATTGTCTCTC CAGCTGTGTA 
GACCGCTTCA TTGACACCAC TCTTGCCATC ACCAGTCGGT TTGCCCAGAT TGTACAGAAA 
GGAGGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036591.3
CDS	34..285
Translation	MAELGEADEAELQRLVAAEQQKAQFTAQVHHFMELCWDKCVEKPGNRLDSRTENCLSSCVDRFIDTTLAITSRFAQIVQKGGQ

Target ORF information:

RefSeq Version	NM_012459.4
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012459.4

ORF Insert Sequence:

ATGGCGGAGC TGGGCGAAGC CGATGAAGCG GAGTTGCAGC GCCTGGTGGC CGCCGAGCAG 
CAGAAGGCGC AGTTTACTGC ACAGGTGCAT CACTTCATGG AGTTATGTTG GGATAAATGT 
GTGGAGAAGC CAGGGAATCG CCTAGACTCT CGCACTGAAA ATTGTCTCTC CAGCTGTGTA 
GACCGCTTCA TTGACACCAC TCTTGCCATC ACCAGTCGGT TTGCCCAGAT TGTACAGAAA 
GGAGGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM8B ( NM_012459.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036591.3 Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107542
Clone ID Related Accession (Same CDS sequence)	NM_012459.4 , NM_012459.3
Accession Version	NM_012459.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit Tim8 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002007.4. On Dec 22, 2018 this sequence version replaced NM_012459.2. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DA784947.1, SRR1660803.379001.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGC TGGGCGAAGC CGATGAAGCG GAGTTGCAGC GCCTGGTGGC CGCCGAGCAG 
CAGAAGGCGC AGTTTACTGC ACAGGTGCAT CACTTCATGG AGTTATGTTG GGATAAATGT 
GTGGAGAAGC CAGGGAATCG CCTAGACTCT CGCACTGAAA ATTGTCTCTC CAGCTGTGTA 
GACCGCTTCA TTGACACCAC TCTTGCCATC ACCAGTCGGT TTGCCCAGAT TGTACAGAAA 
GGAGGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036591.3
CDS	34..285
Translation	MAELGEADEAELQRLVAAEQQKAQFTAQVHHFMELCWDKCVEKPGNRLDSRTENCLSSCVDRFIDTTLAITSRFAQIVQKGGQ

Target ORF information:

RefSeq Version	NM_012459.3
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 8 homolog B (TIMM8B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012459.3

ORF Insert Sequence:

ATGGCGGAGC TGGGCGAAGC CGATGAAGCG GAGTTGCAGC GCCTGGTGGC CGCCGAGCAG 
CAGAAGGCGC AGTTTACTGC ACAGGTGCAT CACTTCATGG AGTTATGTTG GGATAAATGT 
GTGGAGAAGC CAGGGAATCG CCTAGACTCT CGCACTGAAA ATTGTCTCTC CAGCTGTGTA 
GACCGCTTCA TTGACACCAC TCTTGCCATC ACCAGTCGGT TTGCCCAGAT TGTACAGAAA 
GGAGGGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Molecular bioSystems9(6)1108-16(2013 Jun)
Pieragostino D,Agnifili L,Fasanella V,D'Aguanno S,Mastropasqua R,Di Ilio C,Sacchetta P,Urbani A,Del Boccio P

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller.
Molecular cell21(1)123-33(2006 Jan)
Webb CT,Gorman MA,Lazarou M,Ryan MT,Gulbis JM

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TIMM8B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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