GAPDHS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAPDHS
Entrez Gene ID	26330
Full Name	glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Synonyms	GAPD2,GAPDH-2,GAPDS,HEL-S-278,HSD-35
General protein information	Preferred Names glyceraldehyde-3-phosphate dehydrogenase, spermatogenic Names glyceraldehyde-3-phosphate dehydrogenase, testis-specific epididymis secretory protein Li 278 spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008].
Disorder MIM:	609169

mRNA and Protein(s)

mRNA	Protein	Name
XM_017026579.1	XP_016882068.1	glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform X1
NM_014364.4	NP_055179.1	glyceraldehyde-3-phosphate dehydrogenase, testis-specific
NM_014364.5	NP_055179.1	glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Pathways from BioSystems

Homologies

	GAPDHS	XP_001095332.1
Bos taurus (cattle)	GAPDHS	NP_001035642.1
Mus musculus (house mouse)	Gapdhs	NP_032111.1
Danio rerio (zebrafish)	gapdhs	NP_998259.1
Homo sapiens (human)	GAPDHS	NP_055179.1
Canis lupus familiaris (dog)	GAPDHS	XP_533693.2
Rattus norvegicus (Norway rat)	Gapdhs	NP_076454.1
Arabidopsis thaliana (thale cress)	GAPC2	NP_172801.1
Pan troglodytes (chimpanzee)	GAPDHS	XP_512590.2
Kluyveromyces lactis	KLLA0A11858g	XP_451516.1
Eremothecium gossypii	AGOS_AER031C	NP_984891.2
Oryza sativa (rice)	Os04g0486600	NP_001053139.1
Arabidopsis thaliana (thale cress)	GAPC1	NP_187062.1
Oryza sativa (rice)	Os02g0601300	NP_001047348.1

Gene Ontology
Bibliography

Related articles in PubMed

Detection of a mutation in the intron of Sperm-specific glyceraldehyde-3-phosphate dehydrogenase gene in patients with fibrous sheath dysplasia of the sperm flagellum.
Elkina YL, Kuravsky ML, Bragina EE, Kurilo LF, Khayat SS, Sukhomlinova MY, Schmalhausen EV
Andrologia49(2)(2017 Mar)

The proline-rich region of glyceraldehyde-3-phosphate dehydrogenase from human sperm may bind SH3 domains, as revealed by a bioinformatic study of low-complexity protein segments.
Tatjewski M, Gruca A, Plewczynski D, Grynberg M
Molecular reproduction and development83(2)144-8(2016 Feb)

Structural basis for the NAD binding cooperativity and catalytic characteristics of sperm-specific glyceraldehyde-3-phosphate dehydrogenase.
Kuravsky ML, Barinova KV, Asryants RA, Schmalhausen EV, Muronetz VI
Biochimie11528-34(2015 Aug)

Expression analysis of MND1/GAJ, SPATA22, GAPDHS and ACR genes in testicular biopsies from non-obstructive azoospermia (NOA) patients.
Dorosh A, Tepla O, Zatecka E, Ded L, Koci K, Peknicova J
Reproductive biology and endocrinology : RB&E1142(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is assumed that the deficiency in GAPDS observed in most dysplasia of the fibrous sheath sperm samples is ascribable to a disorder in the regulation of GAPDS expression caused by the mutation in the intron region of GAPDS gene.
Title: Detection of a mutation in the intron of Sperm-specific glyceraldehyde-3-phosphate dehydrogenase gene in patients with fibrous sheath dysplasia of the sperm flagellum.

This result implies that GAPDHS participates in specific signal-transduction pathways.
Title: The proline-rich region of glyceraldehyde-3-phosphate dehydrogenase from human sperm may bind SH3 domains, as revealed by a bioinformatic study of low-complexity protein segments.

Structural basis for the NAD binding cooperativity and catalytic characteristics of sperm-specific glyceraldehyde-3-phosphate dehydrogenase
Title: Structural basis for the NAD binding cooperativity and catalytic characteristics of sperm-specific glyceraldehyde-3-phosphate dehydrogenase.

Data indicate that the positive expression of studied genes fertilization rate for GAPDHS positive subset was 66%, ACR - 71%, SPATA22 - 68%, MND1 - 70%, pregnancy rates were 8%, 6%, 18% and 36% respectively.
Title: Expression analysis of MND1/GAJ, SPATA22, GAPDHS and ACR genes in testicular biopsies from non-obstructive azoospermia (NOA) patients.

GAPDS protein was expressed in both Sertoli cells and elongated sperms. The expression of GAPDS gradually increased with age in the epididymis.
Title: Location and characterization of GAPDS in male reproduction.

The following GAPDHS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPDHS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30484	NM_014364.5 Latest version!	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu88359	XM_017026579.1 Latest version!	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu30484	NM_014364.4	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAPDHS ( NM_014364.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055179.1 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.

Price & Availability↑

CloneID	OHu30484
Clone ID Related Accession (Same CDS sequence)	NM_014364.4 , NM_014364.5
Accession Version	NM_014364.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	glyceraldehyde-3-phosphate dehydrogenase, testis-specific
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP370184.1, BC036373.2 and AC002389.1. This sequence is a reference standard in the RefSeqGene project. On Feb 27, 2007 this sequence version replaced NM_014364.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036373.2, GQ472225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAGC GCGACATCGT CCTCACCAAT GTCACCGTTG TCCAGTTGCT GCGACAGCCG 
TGCCCGGTGA CCAGAGCACC GCCCCCACCT GAGCCTAAGG CTGAAGTAGA GCCCCAGCCA 
CAACCAGAGC CCACACCAGT CAGGGAGGAA ATAAAGCCAC CACCGCCACC ACTGCCTCCT 
CACCCCGCTA CTCCTCCTCC TAAGATGGTG TCTGTGGCCC GGGAGCTGAC TGTGGGCATC 
AATGGATTTG GACGCATCGG TCGCCTGGTC CTGCGCGCCT GCATGGAGAA GGGTGTTAAG 
GTGGTGGCTG TGAATGATCC ATTCATTGAC CCGGAATACA TGGTGTACAT GTTTAAGTAT 
GACTCCACCC ACGGCCGATA CAAGGGAAGT GTGGAATTCA GGAATGGACA ACTGGTCGTG 
GACAACCATG AGATCTCTGT CTACCAGTGC AAAGAGCCCA AACAGATCCC CTGGAGGGCT 
GTCGGGAGCC CCTACGTGGT GGAGTCCACA GGCGTGTACC TCTCCATACA GGCAGCTTCG 
GACCACATCT CTGCAGGTGC TCAACGTGTG GTCATCTCCG CGCCCTCACC GGATGCACCA 
ATGTTCGTCA TGGGTGTCAA TGAAAATGAC TATAACCCTG GCTCCATGAA CATTGTGAGC 
AACGCGTCCT GCACCACCAA CTGTTTGGCT CCCCTCGCCA AAGTCATCCA CGAGCGATTT 
GGGATCGTGG AAGGGTTGAT GACCACAGTC CATTCCTACA CGGCCACCCA GAAGACAGTG 
GACGGGCCAT CAAGGAAGGC CTGGCGAGAT GGGCGGGGTG CCCACCAGAA CATCATCCCA 
GCCTCCACTG GGGCTGCGAA AGCTGTGACC AAAGTCATCC CAGAGCTCAA AGGGAAGCTG 
ACAGGGATGG CGTTCCGGGT ACCAACCCCG GATGTGTCTG TCGTGGACCT GACCTGCCGC 
CTCGCCCAGC CTGCCCCCTA CTCAGCCATC AAGGAGGCTG TAAAAGCAGC AGCCAAGGGG 
CCCATGGCTG GCATCCTTGC CTACACCGAG GATGAGGTCG TCTCTACGGA CTTCCTCGGT 
GATACCCACT CGTCCATCTT CGATGCTAAG GCCGGCATTG CGCTCAATGA CAATTTCGTG 
AAGCTCATTT CATGGTACGA CAACGAATAT GGCTACAGTC ACCGGGTGGT CGACCTCCTC 
CGCTACATGT TCAGCCGAGA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055179.1
CDS	117..1343
Translation	MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Target ORF information:

RefSeq Version	NM_014364.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014364.4

ORF Insert Sequence:

ATGTCGAAGC GCGACATCGT CCTCACCAAT GTCACCGTTG TCCAGTTGCT GCGACAGCCG 
TGCCCGGTGA CCAGAGCACC GCCCCCACCT GAGCCTAAGG CTGAAGTAGA GCCCCAGCCA 
CAACCAGAGC CCACACCAGT CAGGGAGGAA ATAAAGCCAC CACCGCCACC ACTGCCTCCT 
CACCCCGCTA CTCCTCCTCC TAAGATGGTG TCTGTGGCCC GGGAGCTGAC TGTGGGCATC 
AATGGATTTG GACGCATCGG TCGCCTGGTC CTGCGCGCCT GCATGGAGAA GGGTGTTAAG 
GTGGTGGCTG TGAATGATCC ATTCATTGAC CCGGAATACA TGGTGTACAT GTTTAAGTAT 
GACTCCACCC ACGGCCGATA CAAGGGAAGT GTGGAATTCA GGAATGGACA ACTGGTCGTG 
GACAACCATG AGATCTCTGT CTACCAGTGC AAAGAGCCCA AACAGATCCC CTGGAGGGCT 
GTCGGGAGCC CCTACGTGGT GGAGTCCACA GGCGTGTACC TCTCCATACA GGCAGCTTCG 
GACCACATCT CTGCAGGTGC TCAACGTGTG GTCATCTCCG CGCCCTCACC GGATGCACCA 
ATGTTCGTCA TGGGTGTCAA TGAAAATGAC TATAACCCTG GCTCCATGAA CATTGTGAGC 
AACGCGTCCT GCACCACCAA CTGTTTGGCT CCCCTCGCCA AAGTCATCCA CGAGCGATTT 
GGGATCGTGG AAGGGTTGAT GACCACAGTC CATTCCTACA CGGCCACCCA GAAGACAGTG 
GACGGGCCAT CAAGGAAGGC CTGGCGAGAT GGGCGGGGTG CCCACCAGAA CATCATCCCA 
GCCTCCACTG GGGCTGCGAA AGCTGTGACC AAAGTCATCC CAGAGCTCAA AGGGAAGCTG 
ACAGGGATGG CGTTCCGGGT ACCAACCCCG GATGTGTCTG TCGTGGACCT GACCTGCCGC 
CTCGCCCAGC CTGCCCCCTA CTCAGCCATC AAGGAGGCTG TAAAAGCAGC AGCCAAGGGG 
CCCATGGCTG GCATCCTTGC CTACACCGAG GATGAGGTCG TCTCTACGGA CTTCCTCGGT 
GATACCCACT CGTCCATCTT CGATGCTAAG GCCGGCATTG CGCTCAATGA CAATTTCGTG 
AAGCTCATTT CATGGTACGA CAACGAATAT GGCTACAGTC ACCGGGTGGT CGACCTCCTC 
CGCTACATGT TCAGCCGAGA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPDHS ( NM_014364.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055179.1 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.

Price & Availability↑

CloneID	OHu30484
Clone ID Related Accession (Same CDS sequence)	NM_014364.4 , NM_014364.5
Accession Version	NM_014364.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	glyceraldehyde-3-phosphate dehydrogenase, testis-specific
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP370184.1, BC036373.2 and AC002389.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014364.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.262758.1, SRR5189667.118236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000222286.9/ ENSP00000222286.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGAAGC GCGACATCGT CCTCACCAAT GTCACCGTTG TCCAGTTGCT GCGACAGCCG 
TGCCCGGTGA CCAGAGCACC GCCCCCACCT GAGCCTAAGG CTGAAGTAGA GCCCCAGCCA 
CAACCAGAGC CCACACCAGT CAGGGAGGAA ATAAAGCCAC CACCGCCACC ACTGCCTCCT 
CACCCCGCTA CTCCTCCTCC TAAGATGGTG TCTGTGGCCC GGGAGCTGAC TGTGGGCATC 
AATGGATTTG GACGCATCGG TCGCCTGGTC CTGCGCGCCT GCATGGAGAA GGGTGTTAAG 
GTGGTGGCTG TGAATGATCC ATTCATTGAC CCGGAATACA TGGTGTACAT GTTTAAGTAT 
GACTCCACCC ACGGCCGATA CAAGGGAAGT GTGGAATTCA GGAATGGACA ACTGGTCGTG 
GACAACCATG AGATCTCTGT CTACCAGTGC AAAGAGCCCA AACAGATCCC CTGGAGGGCT 
GTCGGGAGCC CCTACGTGGT GGAGTCCACA GGCGTGTACC TCTCCATACA GGCAGCTTCG 
GACCACATCT CTGCAGGTGC TCAACGTGTG GTCATCTCCG CGCCCTCACC GGATGCACCA 
ATGTTCGTCA TGGGTGTCAA TGAAAATGAC TATAACCCTG GCTCCATGAA CATTGTGAGC 
AACGCGTCCT GCACCACCAA CTGTTTGGCT CCCCTCGCCA AAGTCATCCA CGAGCGATTT 
GGGATCGTGG AAGGGTTGAT GACCACAGTC CATTCCTACA CGGCCACCCA GAAGACAGTG 
GACGGGCCAT CAAGGAAGGC CTGGCGAGAT GGGCGGGGTG CCCACCAGAA CATCATCCCA 
GCCTCCACTG GGGCTGCGAA AGCTGTGACC AAAGTCATCC CAGAGCTCAA AGGGAAGCTG 
ACAGGGATGG CGTTCCGGGT ACCAACCCCG GATGTGTCTG TCGTGGACCT GACCTGCCGC 
CTCGCCCAGC CTGCCCCCTA CTCAGCCATC AAGGAGGCTG TAAAAGCAGC AGCCAAGGGG 
CCCATGGCTG GCATCCTTGC CTACACCGAG GATGAGGTCG TCTCTACGGA CTTCCTCGGT 
GATACCCACT CGTCCATCTT CGATGCTAAG GCCGGCATTG CGCTCAATGA CAATTTCGTG 
AAGCTCATTT CATGGTACGA CAACGAATAT GGCTACAGTC ACCGGGTGGT CGACCTCCTC 
CGCTACATGT TCAGCCGAGA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055179.1
CDS	73..1299
Translation	MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Target ORF information:

RefSeq Version	NM_014364.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014364.5

ORF Insert Sequence:

ATGTCGAAGC GCGACATCGT CCTCACCAAT GTCACCGTTG TCCAGTTGCT GCGACAGCCG 
TGCCCGGTGA CCAGAGCACC GCCCCCACCT GAGCCTAAGG CTGAAGTAGA GCCCCAGCCA 
CAACCAGAGC CCACACCAGT CAGGGAGGAA ATAAAGCCAC CACCGCCACC ACTGCCTCCT 
CACCCCGCTA CTCCTCCTCC TAAGATGGTG TCTGTGGCCC GGGAGCTGAC TGTGGGCATC 
AATGGATTTG GACGCATCGG TCGCCTGGTC CTGCGCGCCT GCATGGAGAA GGGTGTTAAG 
GTGGTGGCTG TGAATGATCC ATTCATTGAC CCGGAATACA TGGTGTACAT GTTTAAGTAT 
GACTCCACCC ACGGCCGATA CAAGGGAAGT GTGGAATTCA GGAATGGACA ACTGGTCGTG 
GACAACCATG AGATCTCTGT CTACCAGTGC AAAGAGCCCA AACAGATCCC CTGGAGGGCT 
GTCGGGAGCC CCTACGTGGT GGAGTCCACA GGCGTGTACC TCTCCATACA GGCAGCTTCG 
GACCACATCT CTGCAGGTGC TCAACGTGTG GTCATCTCCG CGCCCTCACC GGATGCACCA 
ATGTTCGTCA TGGGTGTCAA TGAAAATGAC TATAACCCTG GCTCCATGAA CATTGTGAGC 
AACGCGTCCT GCACCACCAA CTGTTTGGCT CCCCTCGCCA AAGTCATCCA CGAGCGATTT 
GGGATCGTGG AAGGGTTGAT GACCACAGTC CATTCCTACA CGGCCACCCA GAAGACAGTG 
GACGGGCCAT CAAGGAAGGC CTGGCGAGAT GGGCGGGGTG CCCACCAGAA CATCATCCCA 
GCCTCCACTG GGGCTGCGAA AGCTGTGACC AAAGTCATCC CAGAGCTCAA AGGGAAGCTG 
ACAGGGATGG CGTTCCGGGT ACCAACCCCG GATGTGTCTG TCGTGGACCT GACCTGCCGC 
CTCGCCCAGC CTGCCCCCTA CTCAGCCATC AAGGAGGCTG TAAAAGCAGC AGCCAAGGGG 
CCCATGGCTG GCATCCTTGC CTACACCGAG GATGAGGTCG TCTCTACGGA CTTCCTCGGT 
GATACCCACT CGTCCATCTT CGATGCTAAG GCCGGCATTG CGCTCAATGA CAATTTCGTG 
AAGCTCATTT CATGGTACGA CAACGAATAT GGCTACAGTC ACCGGGTGGT CGACCTCCTC 
CGCTACATGT TCAGCCGAGA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPDHS ( XM_017026579.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882068.1 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88359
Clone ID Related Accession (Same CDS sequence)	XM_017026579.1
Accession Version	XM_017026579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCAG GCCTCTGGCT TGGGCGCCGC CCCTCGGCGA CGTCACTGGG TACTGTGACG 
TCACTGGGTG CTGTGACATC AGGGCAATTA GCCCAGGACC CACAGCCCTG GCGCTCCGCA 
CGCACCTCGG TAACATCACA GCAGGTCCAG GCCAATGATA ACCTTATAAG AGGCCATGTC 
GAAGCGCGAC ATCGTCCTCA CCAATGTCAC CGTTGTCCAG TTGCTGCGAC AGCCGTGCCC 
GGATTTGGAC GCATCGGTCG CCTGGTCCTG CGCGCCTGCA TGGAGAAGGG TGTTAAGGTG 
GTGGCTGTGA ATGATCCATT CATTGACCCG GAATACATGG TGTACATGTT TAAGTATGAC 
TCCACCCACG GCCGATACAA GGGAAGTGTG GAATTCAGGA ATGGACAACT GGTCGTGGAC 
AACCATGAGA TCTCTGTCTA CCAGTGCAAA GAGCCCAAAC AGATCCCCTG GAGGGCTGTC 
GGGAGCCCCT ACGTGGTGGA GTCCACAGGC GTGTACCTCT CCATACAGGC AGCTTCGGAC 
CACATCTCTG CAGGTGCTCA ACGTGTGGTC ATCTCCGCGC CCTCACCGGA TGCACCAATG 
TTCGTCATGG GTGTCAATGA AAATGACTAT AACCCTGGCT CCATGAACAT TGTGAGCAAC 
GCGTCCTGCA CCACCAACTG TTTGGCTCCC CTCGCCAAAG TCATCCACGA GCGATTTGGG 
ATCGTGGAAG GGTTGATGAC CACAGTCCAT TCCTACACGG CCACCCAGAA GACAGTGGAC 
GGGCCATCAA GGAAGGCCTG GCGAGATGGG CGGGGTGCCC ACCAGAACAT CATCCCAGCC 
TCCACTGGGG CTGCGAAAGC TGTGACCAAA GTCATCCCAG AGCTCAAAGG GTATGAGGAC 
AAGAAGCTGC AACCAGGGTG GGGGCATACG CCAGGAGGAC TGGACTGGCC CGGCCCTCAG 
TCCTTAAGAG GAAAGCAGGG GCCTGGCCCA GCCACAGGGA AAGGGGGAAT GGAGGGCAAC 
GTCCCTAAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882068.1
CDS	16..1050
Translation	MVSGLWLGRRPSATSLGTVTSLGAVTSGQLAQDPQPWRSARTSVTSQQVQANDNLIRGHVEARHRPHQCHRCPVAATAVPGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGYEDKKLQPGWGHTPGGLDWPGPQSLRGKQGPGPATGKGGMEGNVPKF

Target ORF information:

RefSeq Version	XM_017026579.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026579.1

ORF Insert Sequence:

ATGGTGTCAG GCCTCTGGCT TGGGCGCCGC CCCTCGGCGA CGTCACTGGG TACTGTGACG 
TCACTGGGTG CTGTGACATC AGGGCAATTA GCCCAGGACC CACAGCCCTG GCGCTCCGCA 
CGCACCTCGG TAACATCACA GCAGGTCCAG GCCAATGATA ACCTTATAAG AGGCCATGTC 
GAAGCGCGAC ATCGTCCTCA CCAATGTCAC CGTTGTCCAG TTGCTGCGAC AGCCGTGCCC 
GGATTTGGAC GCATCGGTCG CCTGGTCCTG CGCGCCTGCA TGGAGAAGGG TGTTAAGGTG 
GTGGCTGTGA ATGATCCATT CATTGACCCG GAATACATGG TGTACATGTT TAAGTATGAC 
TCCACCCACG GCCGATACAA GGGAAGTGTG GAATTCAGGA ATGGACAACT GGTCGTGGAC 
AACCATGAGA TCTCTGTCTA CCAGTGCAAA GAGCCCAAAC AGATCCCCTG GAGGGCTGTC 
GGGAGCCCCT ACGTGGTGGA GTCCACAGGC GTGTACCTCT CCATACAGGC AGCTTCGGAC 
CACATCTCTG CAGGTGCTCA ACGTGTGGTC ATCTCCGCGC CCTCACCGGA TGCACCAATG 
TTCGTCATGG GTGTCAATGA AAATGACTAT AACCCTGGCT CCATGAACAT TGTGAGCAAC 
GCGTCCTGCA CCACCAACTG TTTGGCTCCC CTCGCCAAAG TCATCCACGA GCGATTTGGG 
ATCGTGGAAG GGTTGATGAC CACAGTCCAT TCCTACACGG CCACCCAGAA GACAGTGGAC 
GGGCCATCAA GGAAGGCCTG GCGAGATGGG CGGGGTGCCC ACCAGAACAT CATCCCAGCC 
TCCACTGGGG CTGCGAAAGC TGTGACCAAA GTCATCCCAG AGCTCAAAGG GTATGAGGAC 
AAGAAGCTGC AACCAGGGTG GGGGCATACG CCAGGAGGAC TGGACTGGCC CGGCCCTCAG 
TCCTTAAGAG GAAAGCAGGG GCCTGGCCCA GCCACAGGGA AAGGGGGAAT GGAGGGCAAC 
GTCCCTAAGT TCTGA