ERAL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERAL1
Entrez Gene ID	26284
Full Name	Era like 12S mitochondrial rRNA chaperone 1
Synonyms	CEGA,ERA,ERA-W,ERAL1A,ERAL1B,H-ERA,HERA-A,HERA-B,PRLTS6
General protein information	Preferred Names Era like 12S mitochondrial rRNA chaperone 1 Names GTPase Era, mitochondrial ERA-like protein 1 Era G-protein-like 1 GTP-binding protein era homolog GTPase, human homolog of E. coli essential cell cycle protein Era conserved ERA-like GTPase era (E. coli G-protein homolog)-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015].
Disorder MIM:	607435
Disorder Html:	Perrault syndrome 6, 617565 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005702.2	NP_005693.1	GTPase Era, mitochondrial
NM_001317985.1	NP_001304914.1	GTPase Era, mitochondrial isoform 2
NM_001317986.2	NP_001304915.1	GTPase Era, mitochondrial isoform 3
NM_001317986.1	NP_001304915.1	GTPase Era, mitochondrial isoform 3
NM_005702.4	NP_005693.1	GTPase Era, mitochondrial isoform 1
NM_001317985.2	NP_001304914.1	GTPase Era, mitochondrial isoform 2
NM_005702.3	NP_005693.1	GTPase Era, mitochondrial isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ERAL1	NP_001092492.1
Mus musculus (house mouse)	Eral1	NP_071708.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010622	XP_310115.3
Caenorhabditis elegans (roundworm)	E02H1.2	NP_496060.1
Pan troglodytes (chimpanzee)	ERAL1	XP_511365.3
	LOC716483	XP_001107234.2
Canis lupus familiaris (dog)	ERAL1	XP_537749.3
Drosophila melanogaster (fruit fly)	CG7488	NP_650248.1
Gallus gallus (chicken)	ERAL1	NP_989570.1
Danio rerio (zebrafish)	eral1	NP_001122219.1
Homo sapiens (human)	ERAL1	NP_005693.1
Rattus norvegicus (Norway rat)	Eral1	NP_001013247.1
Xenopus tropicalis (tropical clawed frog)	LOC100495069	XP_002938122.2

Gene Ontology
Bibliography

Related articles in PubMed

A homozygous missense mutation in ERAL1, encoding a mitochondrial rRNA chaperone, causes Perrault syndrome.
Chatzispyrou IA, Alders M, Guerrero-Castillo S, Zapata Perez R, Haagmans MA, Mouchiroud L, Koster J, Ofman R, Baas F, Waterham HR, Spelbrink JN, Auwerx J, Mannens MM, Houtkooper RH, Plomp AS
Human molecular genetics26(13)2541-2550(2017 07)

Autophagy is induced through the ROS-TP53-DRAM1 pathway in response to mitochondrial protein synthesis inhibition.
Xie X, Le L, Fan Y, Lv L, Zhang J
Autophagy8(7)1071-84(2012 Jul)

The role of TFAM-associated proteins in mitochondrial RNA metabolism.
Uchiumi T, Kang D
Biochimica et biophysica acta1820(5)565-70(2012 May)

Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.
Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN
The Biochemical journal430(3)551-8(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Three women from three different families presented with signs of PS, including deafness and ovarian dysgenesis. We employed exome sequencing and identified a homozygous missense mutation in the ERAL1 gene, c.707A > T. The mutation leads to a damaging p.Asn236Ile substitution that is likely to interfere with the GTP binding capacity of the ERAL1 protein.
Title: A homozygous missense mutation in ERAL1, encoding a mitochondrial rRNA chaperone, causes Perrault syndrome.

Data show that loss of ERAL1 leads to rapid decay of nascent 12S mt-rRNA, consistent with a role as a mitochondrial RNA chaperone.
Title: Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.

The following ERAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30909	NM_005702.4 Latest version!	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu100236	NM_001317985.2 Latest version!	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu100242	NM_001317986.2 Latest version!	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu100242	NM_001317986.1	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu30909	NM_005702.2	Homo sapiens Era-like 12S mitochondrial rRNA chaperone 1 (ERAL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu100236	NM_001317985.1	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu30909	NM_005702.3	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERAL1 ( NM_005702.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005693.1 Homo sapiens Era-like 12S mitochondrial rRNA chaperone 1 (ERAL1), mRNA.

Price & Availability↑

CloneID	OHu30909
Clone ID Related Accession (Same CDS sequence)	NM_005702.2 , NM_005702.4 , NM_005702.3
Accession Version	NM_005702.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	GTPase Era, mitochondrial
Comment

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_005693.1

Translation

MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_005702.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens Era-like 12S mitochondrial rRNA chaperone 1 (ERAL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005702.2

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_005702.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005693.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30909
Clone ID Related Accession (Same CDS sequence)	NM_005702.2 , NM_005702.4 , NM_005702.3
Accession Version	NM_005702.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, BC019094.2 and BM683046.1. On Nov 23, 2018 this sequence version replaced NM_005702.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK023342.1, SRR3476690.251267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000254928.10/ ENSP00000254928.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005693.1
CDS	20..1333
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_005702.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005702.4

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_005702.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005693.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30909
Clone ID Related Accession (Same CDS sequence)	NM_005702.2 , NM_005702.4 , NM_005702.3
Accession Version	NM_005702.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, BC019094.2 and BM683046.1. On Dec 18, 2015 this sequence version replaced NM_005702.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.409952.1, SRR1803616.219604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005693.1
CDS	79..1392
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_005702.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005702.3

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GGTAGATTGT 
TTGAAGCAGA AGTCAGTTCT CCTGGAGCTC ACGGCAGCCC TCACTGAAGG TGTGGTCAAT 
GGCAAAAAGC TCAAGATGAG GCAGGCCTTC CACTCACACC CTGGCACCCA TTGCCCCAGC 
CCAGCAGTTA AGGACCCAAA CACACAATCT GTGGGAAATC CTCAGAGGAT TGGCTGGCCC 
CACTTCAAGG AGATCTTCAT GTTGTCAGCC CTAAGCCAGG AGGACGTGAA AACACTAAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGG CCCTGGGAGT ACCACAGTGC AGTCCTCACT 
AGCCAGACAC CAGAAGAGAT CTGTGCCAAC ATTATCCGAG AGAAGCTCCT AGAACACCTG 
CCCCAGGAGG TGCCTTACAA TGTACAGCAG AAGACAGCAG TGTGGGAGGA AGGACCAGGT 
GGGGAGCTGG TTATCCAACA GAAGCTTCTG GTGCCCAAAG AATCTTATGT GAAACTCCTG 
ATTGGTCCGA AGGGCCACGT GATCTCCCAG ATAGCACAGG AGGCAGGCCA TGACCTCATG 
GACATCTTCC TCTGCGATGT TGACATCCGC CTCTCTGTGA AGCTCCTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_001317985.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304914.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100236
Clone ID Related Accession (Same CDS sequence)	NM_001317985.1 , NM_001317985.2
Accession Version	NM_001317985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, AK023342.1, BX384612.2, BC019094.2 and BM683046.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.3746.1, SRR1803613.62330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGATTC TACTTGACAC ACCTGGCATT ATCAGTCCTG GTAAACAGAA GAGGCATCAC 
CTGGAGCTCT CTTTGTTGGA AGATCCATGG AAGAGCATGG AATCTGCTGA TCTTGTTGTG 
GTTCTTGTGG ATGTCTCAGA CAAGTGGACA CGGAACCAGC TCAGCCCCCA GTTGCTCAGG 
TGCTTGACCA AGTACTCCCA GATCCCTAGT GTCCTGGTCA TGAACAAGGT AGATTGTTTG 
AAGCAGAAGT CAGTTCTCCT GGAGCTCACG GCAGCCCTCA CTGAAGGTGT GGTCAATGGC 
AAAAAGCTCA AGATGAGGCA GGCCTTCCAC TCACACCCTG GCACCCATTG CCCCAGCCCA 
GCAGTTAAGG ACCCAAACAC ACAATCTGTG GGAAATCCTC AGAGGATTGG CTGGCCCCAC 
TTCAAGGAGA TCTTCATGTT GTCAGCCCTA AGCCAGGAGG ACGTGAAAAC ACTAAAGCAA 
TACCTTCTGA CACAGGCCCA GCCAGGGCCC TGGGAGTACC ACAGTGCAGT CCTCACTAGC 
CAGACACCAG AAGAGATCTG TGCCAACATT ATCCGAGAGA AGCTCCTAGA ACACCTGCCC 
CAGGAGGTGC CTTACAATGT ACAGCAGAAG ACAGCAGTGT GGGAGGAAGG ACCAGGTGGG 
GAGCTGGTTA TCCAACAGAA GCTTCTGGTG CCCAAAGAAT CTTATGTGAA ACTCCTGATT 
GGTCCGAAGG GCCACGTGAT CTCCCAGATA GCACAGGAGG CAGGCCATGA CCTCATGGAC 
ATCTTCCTCT GCGATGTTGA CATCCGCCTC TCTGTGAAGC TCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304914.1
CDS	79..1389
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_001317985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317985.1

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGATTC TACTTGACAC ACCTGGCATT ATCAGTCCTG GTAAACAGAA GAGGCATCAC 
CTGGAGCTCT CTTTGTTGGA AGATCCATGG AAGAGCATGG AATCTGCTGA TCTTGTTGTG 
GTTCTTGTGG ATGTCTCAGA CAAGTGGACA CGGAACCAGC TCAGCCCCCA GTTGCTCAGG 
TGCTTGACCA AGTACTCCCA GATCCCTAGT GTCCTGGTCA TGAACAAGGT AGATTGTTTG 
AAGCAGAAGT CAGTTCTCCT GGAGCTCACG GCAGCCCTCA CTGAAGGTGT GGTCAATGGC 
AAAAAGCTCA AGATGAGGCA GGCCTTCCAC TCACACCCTG GCACCCATTG CCCCAGCCCA 
GCAGTTAAGG ACCCAAACAC ACAATCTGTG GGAAATCCTC AGAGGATTGG CTGGCCCCAC 
TTCAAGGAGA TCTTCATGTT GTCAGCCCTA AGCCAGGAGG ACGTGAAAAC ACTAAAGCAA 
TACCTTCTGA CACAGGCCCA GCCAGGGCCC TGGGAGTACC ACAGTGCAGT CCTCACTAGC 
CAGACACCAG AAGAGATCTG TGCCAACATT ATCCGAGAGA AGCTCCTAGA ACACCTGCCC 
CAGGAGGTGC CTTACAATGT ACAGCAGAAG ACAGCAGTGT GGGAGGAAGG ACCAGGTGGG 
GAGCTGGTTA TCCAACAGAA GCTTCTGGTG CCCAAAGAAT CTTATGTGAA ACTCCTGATT 
GGTCCGAAGG GCCACGTGAT CTCCCAGATA GCACAGGAGG CAGGCCATGA CCTCATGGAC 
ATCTTCCTCT GCGATGTTGA CATCCGCCTC TCTGTGAAGC TCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_001317985.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304914.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100236
Clone ID Related Accession (Same CDS sequence)	NM_001317985.1 , NM_001317985.2
Accession Version	NM_001317985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, AK023342.1, BX384612.2, BC019094.2 and BM683046.1. On May 31, 2019 this sequence version replaced NM_001317985.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.3746.1, SRR1803612.99015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGATTC TACTTGACAC ACCTGGCATT ATCAGTCCTG GTAAACAGAA GAGGCATCAC 
CTGGAGCTCT CTTTGTTGGA AGATCCATGG AAGAGCATGG AATCTGCTGA TCTTGTTGTG 
GTTCTTGTGG ATGTCTCAGA CAAGTGGACA CGGAACCAGC TCAGCCCCCA GTTGCTCAGG 
TGCTTGACCA AGTACTCCCA GATCCCTAGT GTCCTGGTCA TGAACAAGGT AGATTGTTTG 
AAGCAGAAGT CAGTTCTCCT GGAGCTCACG GCAGCCCTCA CTGAAGGTGT GGTCAATGGC 
AAAAAGCTCA AGATGAGGCA GGCCTTCCAC TCACACCCTG GCACCCATTG CCCCAGCCCA 
GCAGTTAAGG ACCCAAACAC ACAATCTGTG GGAAATCCTC AGAGGATTGG CTGGCCCCAC 
TTCAAGGAGA TCTTCATGTT GTCAGCCCTA AGCCAGGAGG ACGTGAAAAC ACTAAAGCAA 
TACCTTCTGA CACAGGCCCA GCCAGGGCCC TGGGAGTACC ACAGTGCAGT CCTCACTAGC 
CAGACACCAG AAGAGATCTG TGCCAACATT ATCCGAGAGA AGCTCCTAGA ACACCTGCCC 
CAGGAGGTGC CTTACAATGT ACAGCAGAAG ACAGCAGTGT GGGAGGAAGG ACCAGGTGGG 
GAGCTGGTTA TCCAACAGAA GCTTCTGGTG CCCAAAGAAT CTTATGTGAA ACTCCTGATT 
GGTCCGAAGG GCCACGTGAT CTCCCAGATA GCACAGGAGG CAGGCCATGA CCTCATGGAC 
ATCTTCCTCT GCGATGTTGA CATCCGCCTC TCTGTGAAGC TCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304914.1
CDS	20..1330
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_001317985.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317985.2

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGATTC TACTTGACAC ACCTGGCATT ATCAGTCCTG GTAAACAGAA GAGGCATCAC 
CTGGAGCTCT CTTTGTTGGA AGATCCATGG AAGAGCATGG AATCTGCTGA TCTTGTTGTG 
GTTCTTGTGG ATGTCTCAGA CAAGTGGACA CGGAACCAGC TCAGCCCCCA GTTGCTCAGG 
TGCTTGACCA AGTACTCCCA GATCCCTAGT GTCCTGGTCA TGAACAAGGT AGATTGTTTG 
AAGCAGAAGT CAGTTCTCCT GGAGCTCACG GCAGCCCTCA CTGAAGGTGT GGTCAATGGC 
AAAAAGCTCA AGATGAGGCA GGCCTTCCAC TCACACCCTG GCACCCATTG CCCCAGCCCA 
GCAGTTAAGG ACCCAAACAC ACAATCTGTG GGAAATCCTC AGAGGATTGG CTGGCCCCAC 
TTCAAGGAGA TCTTCATGTT GTCAGCCCTA AGCCAGGAGG ACGTGAAAAC ACTAAAGCAA 
TACCTTCTGA CACAGGCCCA GCCAGGGCCC TGGGAGTACC ACAGTGCAGT CCTCACTAGC 
CAGACACCAG AAGAGATCTG TGCCAACATT ATCCGAGAGA AGCTCCTAGA ACACCTGCCC 
CAGGAGGTGC CTTACAATGT ACAGCAGAAG ACAGCAGTGT GGGAGGAAGG ACCAGGTGGG 
GAGCTGGTTA TCCAACAGAA GCTTCTGGTG CCCAAAGAAT CTTATGTGAA ACTCCTGATT 
GGTCCGAAGG GCCACGTGAT CTCCCAGATA GCACAGGAGG CAGGCCATGA CCTCATGGAC 
ATCTTCCTCT GCGATGTTGA CATCCGCCTC TCTGTGAAGC TCCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_001317986.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304915.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100242
Clone ID Related Accession (Same CDS sequence)	NM_001317986.2 , NM_001317986.1
Accession Version	NM_001317986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, AK023342.1, BI758329.1, AC024267.18, BC019094.2 and BM683046.1. On May 31, 2019 this sequence version replaced NM_001317986.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.228972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GCAATACCTT 
CTGACACAGG CCCAGCCAGG GCCCTGGGAG TACCACAGTG CAGTCCTCAC TAGCCAGACA 
CCAGAAGAGA TCTGTGCCAA CATTATCCGA GAGAAGCTCC TAGAACACCT GCCCCAGGAG 
GTGCCTTACA ATGTACAGCA GAAGACAGCA GTGTGGGAGG AAGGACCAGG TGGGGAGCTG 
GTTATCCAAC AGAAGCTTCT GGTGCCCAAA GAATCTTATG TGAAACTCCT GATTGGTCCG 
AAGGGCCACG TGATCTCCCA GATAGCACAG GAGGCAGGCC ATGACCTCAT GGACATCTTC 
CTCTGCGATG TTGACATCCG CCTCTCTGTG AAGCTCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304915.1
CDS	20..1084
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_001317986.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317986.2

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GCAATACCTT 
CTGACACAGG CCCAGCCAGG GCCCTGGGAG TACCACAGTG CAGTCCTCAC TAGCCAGACA 
CCAGAAGAGA TCTGTGCCAA CATTATCCGA GAGAAGCTCC TAGAACACCT GCCCCAGGAG 
GTGCCTTACA ATGTACAGCA GAAGACAGCA GTGTGGGAGG AAGGACCAGG TGGGGAGCTG 
GTTATCCAAC AGAAGCTTCT GGTGCCCAAA GAATCTTATG TGAAACTCCT GATTGGTCCG 
AAGGGCCACG TGATCTCCCA GATAGCACAG GAGGCAGGCC ATGACCTCAT GGACATCTTC 
CTCTGCGATG TTGACATCCG CCTCTCTGTG AAGCTCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERAL1 ( NM_001317986.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304915.1 Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100242
Clone ID Related Accession (Same CDS sequence)	NM_001317986.2 , NM_001317986.1
Accession Version	NM_001317986.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	GTPase Era, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA559976.1, AK023342.1, BI758329.1, AC024267.18, BC019094.2 and BM683046.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.228972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GCAATACCTT 
CTGACACAGG CCCAGCCAGG GCCCTGGGAG TACCACAGTG CAGTCCTCAC TAGCCAGACA 
CCAGAAGAGA TCTGTGCCAA CATTATCCGA GAGAAGCTCC TAGAACACCT GCCCCAGGAG 
GTGCCTTACA ATGTACAGCA GAAGACAGCA GTGTGGGAGG AAGGACCAGG TGGGGAGCTG 
GTTATCCAAC AGAAGCTTCT GGTGCCCAAA GAATCTTATG TGAAACTCCT GATTGGTCCG 
AAGGGCCACG TGATCTCCCA GATAGCACAG GAGGCAGGCC ATGACCTCAT GGACATCTTC 
CTCTGCGATG TTGACATCCG CCTCTCTGTG AAGCTCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304915.1
CDS	79..1143
Translation	MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK

Target ORF information:

RefSeq Version	NM_001317986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Era like 12S mitochondrial rRNA chaperone 1 (ERAL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317986.1

ORF Insert Sequence:

ATGGCTGCCC CCAGCTGGCG CGGGGCTAGG CTTGTTCAAT CGGTGTTAAG AGTCTGGCAG 
GTGGGCCCTC ATGTCGCGAG GGAGCGGGTG ATCCCTTTTT CCTCACTCTT AGGCTTCCAA 
CGGAGGTGCG TGTCCTGCGT CGCGGGGTCC GCTTTCTCTG GTCCCCGCTT GGCCTCGGCT 
TCTCGCAGTA ATGGCCAGGG CTCTGCCCTG GACCACTTCC TCGGATTCTC TCAGCCCGAC 
AGTTCGGTGA CTCCTTGCGT CCCCGCGGTG TCCATGAACA GAGATGAGCA GGATGTCCTC 
TTGGTCCATC ACCCTGATAT GCCTGAGAAT TCCCGGGTCC TACGAGTGGT CCTCCTGGGA 
GCCCCGAATG CAGGGAAGTC AACACTCTCC AACCAGCTAC TGGGCCGAAA GGTGTTCCCT 
GTTTCCAGGA AGGTGCATAC TACTCGCTGC CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAGGTGA TTCTACTTGA CACACCTGGC ATTATCAGTC CTGGTAAACA GAAGAGGCAT 
CACCTGGAGC TCTCTTTGTT GGAAGATCCA TGGAAGAGCA TGGAATCTGC TGATCTTGTT 
GTGGTTCTTG TGGATGTCTC AGACAAGTGG ACACGGAACC AGCTCAGCCC CCAGTTGCTC 
AGGTGCTTGA CCAAGTACTC CCAGATCCCT AGTGTCCTGG TCATGAACAA GCAATACCTT 
CTGACACAGG CCCAGCCAGG GCCCTGGGAG TACCACAGTG CAGTCCTCAC TAGCCAGACA 
CCAGAAGAGA TCTGTGCCAA CATTATCCGA GAGAAGCTCC TAGAACACCT GCCCCAGGAG 
GTGCCTTACA ATGTACAGCA GAAGACAGCA GTGTGGGAGG AAGGACCAGG TGGGGAGCTG 
GTTATCCAAC AGAAGCTTCT GGTGCCCAAA GAATCTTATG TGAAACTCCT GATTGGTCCG 
AAGGGCCACG TGATCTCCCA GATAGCACAG GAGGCAGGCC ATGACCTCAT GGACATCTTC 
CTCTGCGATG TTGACATCCG CCTCTCTGTG AAGCTCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A homozygous missense mutation in ERAL1, encoding a mitochondrial rRNA chaperone, causes Perrault syndrome.
Human molecular genetics26(13)2541-2550(2017 07)
Chatzispyrou IA,Alders M,Guerrero-Castillo S,Zapata Perez R,Haagmans MA,Mouchiroud L,Koster J,Ofman R,Baas F,Waterham HR,Spelbrink JN,Auwerx J,Mannens MM,Houtkooper RH,Plomp AS

Autophagy is induced through the ROS-TP53-DRAM1 pathway in response to mitochondrial protein synthesis inhibition.
Autophagy8(7)1071-84(2012 Jul)
Xie X,Le L,Fan Y,Lv L,Zhang J

The role of TFAM-associated proteins in mitochondrial RNA metabolism.
Biochimica et biophysica acta1820(5)565-70(2012 May)
Uchiumi T,Kang D

Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.
The Biochemical journal430(3)551-8(2010 Sep)
Dennerlein S,Rozanska A,Wydro M,Chrzanowska-Lightowlers ZM,Lightowlers RN

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ERAL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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