GARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GARS1
Entrez Gene ID	2617
Full Name	glycyl-tRNA synthetase
Synonyms	CMT2D,DSMAV,GlyRS,HMN5,SMAD1
General protein information	Preferred Names glycyl-tRNA synthetase Names glycine--tRNA ligase AP-4-A synthetase Charcot-Marie-Tooth neuropathy 2D Charcot-Marie-Tooth neuropathy, neuronal type, D diadenosine tetraphosphate synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p14.3
Summary	This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	600287
Disorder Html:	Charcot-Marie-Tooth disease, type 2D, 601472 (3); Neuropathy, distal hereditary motor, type VA, 600794 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002047.3	NP_002038.2	glycine--tRNA ligase isoform 1 precursor
NM_002047.4	NP_002038.2	glycine--tRNA ligase isoform 1 precursor
NM_001316772.1	NP_001303701.1	glycine--tRNA ligase isoform 2
XM_006715686.2	XP_006715749.1	glycine--tRNA ligase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GARS	NP_002038.2
Drosophila melanogaster (fruit fly)	Aats-gly	NP_001261867.1
Saccharomyces cerevisiae (baker's yeast)	GRS1	NP_009679.2
Magnaporthe oryzae (rice blast fungus)	MGG_06321	XP_003717264.1
Neurospora crassa	NCU00405	XP_957291.1
Xenopus tropicalis (tropical clawed frog)	gars	NP_001007492.1
Pan troglodytes (chimpanzee)	GARS	XP_519025.3
Bos taurus (cattle)	GARS	NP_001091035.1
Rattus norvegicus (Norway rat)	Gars	NP_001258068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008604	XP_557743.2
Caenorhabditis elegans (roundworm)	gars-1	NP_498093.1
Gallus gallus (chicken)	GARS	NP_001026681.1
Oryza sativa (rice)	Os08g0538000	NP_001062369.1
Kluyveromyces lactis	KLLA0B08932g	XP_451926.1
Eremothecium gossypii	AGOS_ADL373W	NP_983723.1
Schizosaccharomyces pombe (fission yeast)	grs1	NP_593935.1
Canis lupus familiaris (dog)	GARS	XP_003639538.1
Mus musculus (house mouse)	Gars	NP_851009.2
Danio rerio (zebrafish)	gars	XP_692410.4
Arabidopsis thaliana (thale cress)	AT1G29880	NP_564337.1

Gene Ontology
Bibliography

Related articles in PubMed

Compound heterozygosity for loss-of-function GARS variants results in a multisystem developmental syndrome that includes severe growth retardation.
Oprescu SN, Chepa-Lotrea X, Takase R, Golas G, Markello TC, Adams DR, Toro C, Gropman AL, Hou YM, Malicdan MCV, Gahl WA, Tifft CJ, Antonellis A
Human mutation38(10)1412-1420(2017 Oct)

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Sleigh JN, G?mez-Mart?n A, Wei N, Bai G, Yang XL, Schiavo G
Scientific reports7(1)9216(2017 Aug)

Crystal Structure of the Wild-Type Human GlyRS Bound with tRNA(Gly) in a Productive Conformation.
Qin X, Deng X, Chen L, Xie W
Journal of molecular biology428(18)3603-14(2016 Sep)

Neddylation requires glycyl-tRNA synthetase to protect activated E2.
Mo Z, Zhang Q, Liu Z, Lauer J, Shi Y, Sun L, Griffin PR, Yang XL
Nature structural & molecular biology23(8)730-7(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

At the active site, a glycyl-AMP molecule is synthesized and is waiting for the transfer of the glycyl moiety to occur.
Title: Crystal Structure of the Wild-Type Human GlyRS Bound with tRNA(Gly) in a Productive Conformation.

GlyRS functions as a chaperone that critically supports neddylation.
Title: Neddylation requires glycyl-tRNA synthetase to protect activated E2.

Data indicate that dimerization is required for the dominant neurotoxicity of disease-associated GARS mutations and provide a rapid, tractable model for studying newly identified GARS variants for a role in human disease.
Title: Dimerization is required for GARS-mediated neurotoxicity in dominant CMT disease.

This study reports two crystal structures of human GlyRS variants, in the free form and in complex with tRNA(Gly) respectively, and reveal new aspects of the glycylation mechanism.
Title: Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA Synthetase (hGlyRS) during Catalysis.

one of the mRNAs isoforms tightly controls expression and localization of human GARS.
Title: Elaborate uORF/IRES features control expression and localization of human glycyl-tRNA synthetase.

The following GARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu41141	XM_006715686.2 Latest version!	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu101883	NM_001316772.1 Latest version!	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu25860	NM_002047.4 Latest version!	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu25860	NM_002047.3	Homo sapiens glycyl-tRNA synthetase (GARS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GARS1 ( XM_006715686.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715749.1 Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu41141
Clone ID Related Accession (Same CDS sequence)	XM_006715686.2
Accession Version	XM_006715686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA CCCTGAAGAG GAGGTTTTTC TATGATCAAG CTTTTGCTAT TTATGGAGGT 
GTTAGTGGTC TGTATGACTT TGGGCCAGTT GGCTGTGCTT TGAAGAACAA TATTATTCAG 
ACCTGGAGGC AGCACTTTAT CCAAGAGGAA CAGATCCTGG AGATCGATTG CACCATGCTC 
ACCCCTGAGC CAGTTTTAAA GACCTCTGGC CATGTAGACA AATTTGCTGA CTTCATGGTG 
AAAGACGTAA AAAATGGAGA ATGTTTTCGT GCTGACCATC TATTAAAAGC TCATTTACAG 
AAATTGATGT CTGATAAGAA GTGTTCTGTC GAAAAGAAAT CAGAAATGGA AAGTGTTTTG 
GCCCAGCTTG ATAACTATGG ACAGCAAGAA CTTGCGGATC TTTTTGTGAA CTATAATGTA 
AAATCTCCCA TTACTGGAAA TGATCTATCC CCTCCAGTGT CTTTTAACTT AATGTTCAAG 
ACTTTCATTG GGCCTGGAGG AAACATGCCT GGGTACTTGA GACCAGAAAC TGCACAGGGG 
ATTTTCTTGA ATTTCAAACG ACTTTTGGAG TTCAACCAAG GAAAGTTGCC TTTTGCTGCT 
GCCCAGATTG GAAATTCTTT TAGAAATGAG ATCTCCCCTC GATCTGGACT GATCAGAGTC 
AGAGAATTCA CAATGGCAGA AATTGAGCAC TTTGTAGATC CCAGTGAGAA AGACCACCCC 
AAGTTCCAGA ATGTGGCAGA CCTTCACCTT TATTTGTATT CAGCAAAAGC CCAGGTCAGC 
GGACAGTCCG CTCGGAAAAT GCGCCTGGGA GATGCTGTTG AACAGGGTGT GATTAATAAC 
ACAGTATTAG GCTATTTCAT TGGCCGCATC TACCTCTACC TCACGAAGGT TGGAATATCT 
CCAGATAAAC TCCGCTTCCG GCAGCACATG GAGAATGAGA TGGCCCATTA TGCCTGTGAC 
TGTTGGGATG CAGAATCCAA AACATCCTAC GGTTGGATTG AGATTGTTGG ATGTGCTGAT 
CGTTCCTGTT ATGACCTCTC CTGTCATGCA CGAGCCACCA AAGTCCCACT TGTAGCTGAG 
AAACCTCTGA AAGAACCCAA AACAGTCAAT GTTGTTCAGT TTGAACCCAG TAAGGGAGCA 
ATTGGTAAGG CATATAAGAA GGATGCAAAA CTGGTGATGG AGTATCTTGC CATTTGTGAT 
GAGTGCTACA TTACAGAAAT GGAGATGCTG CTGAATGAGA AAGGGGAATT CACAATTGAA 
ACTGAAGGGA AAACATTTCA GTTAACAAAA GACATGATCA ATGTGAAGAG ATTCCAGAAA 
ACACTATATG TGGAAGAAGT TGTTCCGAAT GTAATTGAAC CTTCCTTCGG CCTGGGTAGG 
ATCATGTATA CGGTATTTGA ACATACATTC CATGTACGAG AAGGAGATGA ACAGAGAACA 
TTCTTCAGTT TCCCTGCTGT AGTTGCTCCA TTCAAATGTT CCGTCCTCCC ACTGAGCCAA 
AACCAGGAGT TCATGCCATT TGTCAAGGAA TTATCGGAAG CCCTGACCAG GCATGGAGTA 
TCTCACAAAG TAGACGATTC CTCTGGGTCA ATCGGAAGGC GCTATGCCAG GACTGATGAG 
ATTGGCGTGG CTTTTGGTGT CACCATTGAC TTTGACACAG TGAACAAGAC CCCCCACACT 
GCAACTCTGA GGGACCGTGA CTCAATGCGG CAGATAAGAG CAGAGATCTC TGAGCTGCCC 
AGCATAGTCC AAGACCTAGC CAATGGCAAC ATCACATGGG CTGATGTGGA GGCCAGGTAT 
CCTCTGTTTG AAGGGCAAGA GACTGGTAAA AAAGAGACAA TCGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715749.1
CDS	559..2409
Translation	MEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE

Target ORF information:

RefSeq Version	XM_006715686.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715686.2

ORF Insert Sequence:

ATGGAAGATA CCCTGAAGAG GAGGTTTTTC TATGATCAAG CTTTTGCTAT TTATGGAGGT 
GTTAGTGGTC TGTATGACTT TGGGCCAGTT GGCTGTGCTT TGAAGAACAA TATTATTCAG 
ACCTGGAGGC AGCACTTTAT CCAAGAGGAA CAGATCCTGG AGATCGATTG CACCATGCTC 
ACCCCTGAGC CAGTTTTAAA GACCTCTGGC CATGTAGACA AATTTGCTGA CTTCATGGTG 
AAAGACGTAA AAAATGGAGA ATGTTTTCGT GCTGACCATC TATTAAAAGC TCATTTACAG 
AAATTGATGT CTGATAAGAA GTGTTCTGTC GAAAAGAAAT CAGAAATGGA AAGTGTTTTG 
GCCCAGCTTG ATAACTATGG ACAGCAAGAA CTTGCGGATC TTTTTGTGAA CTATAATGTA 
AAATCTCCCA TTACTGGAAA TGATCTATCC CCTCCAGTGT CTTTTAACTT AATGTTCAAG 
ACTTTCATTG GGCCTGGAGG AAACATGCCT GGGTACTTGA GACCAGAAAC TGCACAGGGG 
ATTTTCTTGA ATTTCAAACG ACTTTTGGAG TTCAACCAAG GAAAGTTGCC TTTTGCTGCT 
GCCCAGATTG GAAATTCTTT TAGAAATGAG ATCTCCCCTC GATCTGGACT GATCAGAGTC 
AGAGAATTCA CAATGGCAGA AATTGAGCAC TTTGTAGATC CCAGTGAGAA AGACCACCCC 
AAGTTCCAGA ATGTGGCAGA CCTTCACCTT TATTTGTATT CAGCAAAAGC CCAGGTCAGC 
GGACAGTCCG CTCGGAAAAT GCGCCTGGGA GATGCTGTTG AACAGGGTGT GATTAATAAC 
ACAGTATTAG GCTATTTCAT TGGCCGCATC TACCTCTACC TCACGAAGGT TGGAATATCT 
CCAGATAAAC TCCGCTTCCG GCAGCACATG GAGAATGAGA TGGCCCATTA TGCCTGTGAC 
TGTTGGGATG CAGAATCCAA AACATCCTAC GGTTGGATTG AGATTGTTGG ATGTGCTGAT 
CGTTCCTGTT ATGACCTCTC CTGTCATGCA CGAGCCACCA AAGTCCCACT TGTAGCTGAG 
AAACCTCTGA AAGAACCCAA AACAGTCAAT GTTGTTCAGT TTGAACCCAG TAAGGGAGCA 
ATTGGTAAGG CATATAAGAA GGATGCAAAA CTGGTGATGG AGTATCTTGC CATTTGTGAT 
GAGTGCTACA TTACAGAAAT GGAGATGCTG CTGAATGAGA AAGGGGAATT CACAATTGAA 
ACTGAAGGGA AAACATTTCA GTTAACAAAA GACATGATCA ATGTGAAGAG ATTCCAGAAA 
ACACTATATG TGGAAGAAGT TGTTCCGAAT GTAATTGAAC CTTCCTTCGG CCTGGGTAGG 
ATCATGTATA CGGTATTTGA ACATACATTC CATGTACGAG AAGGAGATGA ACAGAGAACA 
TTCTTCAGTT TCCCTGCTGT AGTTGCTCCA TTCAAATGTT CCGTCCTCCC ACTGAGCCAA 
AACCAGGAGT TCATGCCATT TGTCAAGGAA TTATCGGAAG CCCTGACCAG GCATGGAGTA 
TCTCACAAAG TAGACGATTC CTCTGGGTCA ATCGGAAGGC GCTATGCCAG GACTGATGAG 
ATTGGCGTGG CTTTTGGTGT CACCATTGAC TTTGACACAG TGAACAAGAC CCCCCACACT 
GCAACTCTGA GGGACCGTGA CTCAATGCGG CAGATAAGAG CAGAGATCTC TGAGCTGCCC 
AGCATAGTCC AAGACCTAGC CAATGGCAAC ATCACATGGG CTGATGTGGA GGCCAGGTAT 
CCTCTGTTTG AAGGGCAAGA GACTGGTAAA AAAGAGACAA TCGAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GARS1 ( NM_001316772.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303701.1 Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101883
Clone ID Related Accession (Same CDS sequence)	NM_001316772.1
Accession Version	NM_001316772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	glycine--tRNA ligase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA386636.1, BC007755.2, AC005154.1 and AA464978.1. Transcript Variant: This variant (2) uses an upstream transcription start site compared to variant 1. This variant contains a uORF that was shown in PMID: 26327585 to block translation from the same start codon used in variant 1. Therefore, this variant uses a downstream translation initiation site compared to variant 1 and encodes an isoform (2) that is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.13028.1, AK074524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:26327585) regulatory uORF :: PMID: 26327585 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGGCG CGGGGGCTGA GGAGGTGCTG GCACCTCTGA GGCTAGCAGT GCGCCAGCAG 
GGAGATCTTG TGCGAAAACT CAAAGAAGAT AAAGCACCCC AAGTAGACGT AGACAAAGCA 
GTGGCTGAGC TCAAAGCCCG CAAGAGGGTT CTGGAAGCAA AGGAGCTGGC GTTACAGCCC 
AAAGATGATA TTGTAGACCG AGCAAAAATG GAAGATACCC TGAAGAGGAG GTTTTTCTAT 
GATCAAGCTT TTGCTATTTA TGGAGGTGTT AGTGGTCTGT ATGACTTTGG GCCAGTTGGC 
TGTGCTTTGA AGAACAATAT TATTCAGACC TGGAGGCAGC ACTTTATCCA AGAGGAACAG 
ATCCTGGAGA TCGATTGCAC CATGCTCACC CCTGAGCCAG TTTTAAAGAC CTCTGGCCAT 
GTAGACAAAT TTGCTGACTT CATGGTGAAA GACGTAAAAA ATGGAGAATG TTTTCGTGCT 
GACCATCTAT TAAAAGCTCA TTTACAGAAA TTGATGTCTG ATAAGAAGTG TTCTGTCGAA 
AAGAAATCAG AAATGGAAAG TGTTTTGGCC CAGCTTGATA ACTATGGACA GCAAGAACTT 
GCGGATCTTT TTGTGAACTA TAATGTAAAA TCTCCCATTA CTGGAAATGA TCTATCCCCT 
CCAGTGTCTT TTAACTTAAT GTTCAAGACT TTCATTGGGC CTGGAGGAAA CATGCCTGGG 
TACTTGAGAC CAGAAACTGC ACAGGGGATT TTCTTGAATT TCAAACGACT TTTGGAGTTC 
AACCAAGGAA AGTTGCCTTT TGCTGCTGCC CAGATTGGAA ATTCTTTTAG AAATGAGATC 
TCCCCTCGAT CTGGACTGAT CAGAGTCAGA GAATTCACAA TGGCAGAAAT TGAGCACTTT 
GTAGATCCCA GTGAGAAAGA CCACCCCAAG TTCCAGAATG TGGCAGACCT TCACCTTTAT 
TTGTATTCAG CAAAAGCCCA GGTCAGCGGA CAGTCCGCTC GGAAAATGCG CCTGGGAGAT 
GCTGTTGAAC AGGGTGTGAT TAATAACACA GTATTAGGCT ATTTCATTGG CCGCATCTAC 
CTCTACCTCA CGAAGGTTGG AATATCTCCA GATAAACTCC GCTTCCGGCA GCACATGGAG 
AATGAGATGG CCCATTATGC CTGTGACTGT TGGGATGCAG AATCCAAAAC ATCCTACGGT 
TGGATTGAGA TTGTTGGATG TGCTGATCGT TCCTGTTATG ACCTCTCCTG TCATGCACGA 
GCCACCAAAG TCCCACTTGT AGCTGAGAAA CCTCTGAAAG AACCCAAAAC AGTCAATGTT 
GTTCAGTTTG AACCCAGTAA GGGAGCAATT GGTAAGGCAT ATAAGAAGGA TGCAAAACTG 
GTGATGGAGT ATCTTGCCAT TTGTGATGAG TGCTACATTA CAGAAATGGA GATGCTGCTG 
AATGAGAAAG GGGAATTCAC AATTGAAACT GAAGGGAAAA CATTTCAGTT AACAAAAGAC 
ATGATCAATG TGAAGAGATT CCAGAAAACA CTATATGTGG AAGAAGTTGT TCCGAATGTA 
ATTGAACCTT CCTTCGGCCT GGGTAGGATC ATGTATACGG TATTTGAACA TACATTCCAT 
GTACGAGAAG GAGATGAACA GAGAACATTC TTCAGTTTCC CTGCTGTAGT TGCTCCATTC 
AAATGTTCCG TCCTCCCACT GAGCCAAAAC CAGGAGTTCA TGCCATTTGT CAAGGAATTA 
TCGGAAGCCC TGACCAGGCA TGGAGTATCT CACAAAGTAG ACGATTCCTC TGGGTCAATC 
GGAAGGCGCT ATGCCAGGAC TGATGAGATT GGCGTGGCTT TTGGTGTCAC CATTGACTTT 
GACACAGTGA ACAAGACCCC CCACACTGCA ACTCTGAGGG ACCGTGACTC AATGCGGCAG 
ATAAGAGCAG AGATCTCTGA GCTGCCCAGC ATAGTCCAAG ACCTAGCCAA TGGCAACATC 
ACATGGGCTG ATGTGGAGGC CAGGTATCCT CTGTTTGAAG GGCAAGAGAC TGGTAAAAAA 
GAGACAATCG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303701.1
CDS	350..2407
Translation	MDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE

Target ORF information:

RefSeq Version	NM_001316772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316772.1

ORF Insert Sequence:

ATGGACGGCG CGGGGGCTGA GGAGGTGCTG GCACCTCTGA GGCTAGCAGT GCGCCAGCAG 
GGAGATCTTG TGCGAAAACT CAAAGAAGAT AAAGCACCCC AAGTAGACGT AGACAAAGCA 
GTGGCTGAGC TCAAAGCCCG CAAGAGGGTT CTGGAAGCAA AGGAGCTGGC GTTACAGCCC 
AAAGATGATA TTGTAGACCG AGCAAAAATG GAAGATACCC TGAAGAGGAG GTTTTTCTAT 
GATCAAGCTT TTGCTATTTA TGGAGGTGTT AGTGGTCTGT ATGACTTTGG GCCAGTTGGC 
TGTGCTTTGA AGAACAATAT TATTCAGACC TGGAGGCAGC ACTTTATCCA AGAGGAACAG 
ATCCTGGAGA TCGATTGCAC CATGCTCACC CCTGAGCCAG TTTTAAAGAC CTCTGGCCAT 
GTAGACAAAT TTGCTGACTT CATGGTGAAA GACGTAAAAA ATGGAGAATG TTTTCGTGCT 
GACCATCTAT TAAAAGCTCA TTTACAGAAA TTGATGTCTG ATAAGAAGTG TTCTGTCGAA 
AAGAAATCAG AAATGGAAAG TGTTTTGGCC CAGCTTGATA ACTATGGACA GCAAGAACTT 
GCGGATCTTT TTGTGAACTA TAATGTAAAA TCTCCCATTA CTGGAAATGA TCTATCCCCT 
CCAGTGTCTT TTAACTTAAT GTTCAAGACT TTCATTGGGC CTGGAGGAAA CATGCCTGGG 
TACTTGAGAC CAGAAACTGC ACAGGGGATT TTCTTGAATT TCAAACGACT TTTGGAGTTC 
AACCAAGGAA AGTTGCCTTT TGCTGCTGCC CAGATTGGAA ATTCTTTTAG AAATGAGATC 
TCCCCTCGAT CTGGACTGAT CAGAGTCAGA GAATTCACAA TGGCAGAAAT TGAGCACTTT 
GTAGATCCCA GTGAGAAAGA CCACCCCAAG TTCCAGAATG TGGCAGACCT TCACCTTTAT 
TTGTATTCAG CAAAAGCCCA GGTCAGCGGA CAGTCCGCTC GGAAAATGCG CCTGGGAGAT 
GCTGTTGAAC AGGGTGTGAT TAATAACACA GTATTAGGCT ATTTCATTGG CCGCATCTAC 
CTCTACCTCA CGAAGGTTGG AATATCTCCA GATAAACTCC GCTTCCGGCA GCACATGGAG 
AATGAGATGG CCCATTATGC CTGTGACTGT TGGGATGCAG AATCCAAAAC ATCCTACGGT 
TGGATTGAGA TTGTTGGATG TGCTGATCGT TCCTGTTATG ACCTCTCCTG TCATGCACGA 
GCCACCAAAG TCCCACTTGT AGCTGAGAAA CCTCTGAAAG AACCCAAAAC AGTCAATGTT 
GTTCAGTTTG AACCCAGTAA GGGAGCAATT GGTAAGGCAT ATAAGAAGGA TGCAAAACTG 
GTGATGGAGT ATCTTGCCAT TTGTGATGAG TGCTACATTA CAGAAATGGA GATGCTGCTG 
AATGAGAAAG GGGAATTCAC AATTGAAACT GAAGGGAAAA CATTTCAGTT AACAAAAGAC 
ATGATCAATG TGAAGAGATT CCAGAAAACA CTATATGTGG AAGAAGTTGT TCCGAATGTA 
ATTGAACCTT CCTTCGGCCT GGGTAGGATC ATGTATACGG TATTTGAACA TACATTCCAT 
GTACGAGAAG GAGATGAACA GAGAACATTC TTCAGTTTCC CTGCTGTAGT TGCTCCATTC 
AAATGTTCCG TCCTCCCACT GAGCCAAAAC CAGGAGTTCA TGCCATTTGT CAAGGAATTA 
TCGGAAGCCC TGACCAGGCA TGGAGTATCT CACAAAGTAG ACGATTCCTC TGGGTCAATC 
GGAAGGCGCT ATGCCAGGAC TGATGAGATT GGCGTGGCTT TTGGTGTCAC CATTGACTTT 
GACACAGTGA ACAAGACCCC CCACACTGCA ACTCTGAGGG ACCGTGACTC AATGCGGCAG 
ATAAGAGCAG AGATCTCTGA GCTGCCCAGC ATAGTCCAAG ACCTAGCCAA TGGCAACATC 
ACATGGGCTG ATGTGGAGGC CAGGTATCCT CTGTTTGAAG GGCAAGAGAC TGGTAAAAAA 
GAGACAATCG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GARS1 ( NM_002047.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002038.2 Homo sapiens glycyl-tRNA synthetase (GARS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25860
Clone ID Related Accession (Same CDS sequence)	NM_002047.3 , NM_002047.4
Accession Version	NM_002047.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	glycine--tRNA ligase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA386636.1, AC005154.1, BC007755.2 and AA464978.1. On Oct 28, 2015 this sequence version replaced NM_002047.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.7191.1, AK074524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCTC CGCGTCCAGT GCTGCTTAGA GGTGCTCGCG CCGCTCTGCT GCTGCTGCTG 
CCGCCCCGGC TCTTAGCCCG ACCCTCGCTC CTGCTCCGCC GGTCCCTCAG CGCGGCCTCC 
TGCCCCCCGA TCTCCTTGCC CGCCGCCGCC TCCCGGAGCA GCATGGACGG CGCGGGGGCT 
GAGGAGGTGC TGGCACCTCT GAGGCTAGCA GTGCGCCAGC AGGGAGATCT TGTGCGAAAA 
CTCAAAGAAG ATAAAGCACC CCAAGTAGAC GTAGACAAAG CAGTGGCTGA GCTCAAAGCC 
CGCAAGAGGG TTCTGGAAGC AAAGGAGCTG GCGTTACAGC CCAAAGATGA TATTGTAGAC 
CGAGCAAAAA TGGAAGATAC CCTGAAGAGG AGGTTTTTCT ATGATCAAGC TTTTGCTATT 
TATGGAGGTG TTAGTGGTCT GTATGACTTT GGGCCAGTTG GCTGTGCTTT GAAGAACAAT 
ATTATTCAGA CCTGGAGGCA GCACTTTATC CAAGAGGAAC AGATCCTGGA GATCGATTGC 
ACCATGCTCA CCCCTGAGCC AGTTTTAAAG ACCTCTGGCC ATGTAGACAA ATTTGCTGAC 
TTCATGGTGA AAGACGTAAA AAATGGAGAA TGTTTTCGTG CTGACCATCT ATTAAAAGCT 
CATTTACAGA AATTGATGTC TGATAAGAAG TGTTCTGTCG AAAAGAAATC AGAAATGGAA 
AGTGTTTTGG CCCAGCTTGA TAACTATGGA CAGCAAGAAC TTGCGGATCT TTTTGTGAAC 
TATAATGTAA AATCTCCCAT TACTGGAAAT GATCTATCCC CTCCAGTGTC TTTTAACTTA 
ATGTTCAAGA CTTTCATTGG GCCTGGAGGA AACATGCCTG GGTACTTGAG ACCAGAAACT 
GCACAGGGGA TTTTCTTGAA TTTCAAACGA CTTTTGGAGT TCAACCAAGG AAAGTTGCCT 
TTTGCTGCTG CCCAGATTGG AAATTCTTTT AGAAATGAGA TCTCCCCTCG ATCTGGACTG 
ATCAGAGTCA GAGAATTCAC AATGGCAGAA ATTGAGCACT TTGTAGATCC CAGTGAGAAA 
GACCACCCCA AGTTCCAGAA TGTGGCAGAC CTTCACCTTT ATTTGTATTC AGCAAAAGCC 
CAGGTCAGCG GACAGTCCGC TCGGAAAATG CGCCTGGGAG ATGCTGTTGA ACAGGGTGTG 
ATTAATAACA CAGTATTAGG CTATTTCATT GGCCGCATCT ACCTCTACCT CACGAAGGTT 
GGAATATCTC CAGATAAACT CCGCTTCCGG CAGCACATGG AGAATGAGAT GGCCCATTAT 
GCCTGTGACT GTTGGGATGC AGAATCCAAA ACATCCTACG GTTGGATTGA GATTGTTGGA 
TGTGCTGATC GTTCCTGTTA TGACCTCTCC TGTCATGCAC GAGCCACCAA AGTCCCACTT 
GTAGCTGAGA AACCTCTGAA AGAACCCAAA ACAGTCAATG TTGTTCAGTT TGAACCCAGT 
AAGGGAGCAA TTGGTAAGGC ATATAAGAAG GATGCAAAAC TGGTGATGGA GTATCTTGCC 
ATTTGTGATG AGTGCTACAT TACAGAAATG GAGATGCTGC TGAATGAGAA AGGGGAATTC 
ACAATTGAAA CTGAAGGGAA AACATTTCAG TTAACAAAAG ACATGATCAA TGTGAAGAGA 
TTCCAGAAAA CACTATATGT GGAAGAAGTT GTTCCGAATG TAATTGAACC TTCCTTCGGC 
CTGGGTAGGA TCATGTATAC GGTATTTGAA CATACATTCC ATGTACGAGA AGGAGATGAA 
CAGAGAACAT TCTTCAGTTT CCCTGCTGTA GTTGCTCCAT TCAAATGTTC CGTCCTCCCA 
CTGAGCCAAA ACCAGGAGTT CATGCCATTT GTCAAGGAAT TATCGGAAGC CCTGACCAGG 
CATGGAGTAT CTCACAAAGT AGACGATTCC TCTGGGTCAA TCGGAAGGCG CTATGCCAGG 
ACTGATGAGA TTGGCGTGGC TTTTGGTGTC ACCATTGACT TTGACACAGT GAACAAGACC 
CCCCACACTG CAACTCTGAG GGACCGTGAC TCAATGCGGC AGATAAGAGC AGAGATCTCT 
GAGCTGCCCA GCATAGTCCA AGACCTAGCC AATGGCAACA TCACATGGGC TGATGTGGAG 
GCCAGGTATC CTCTGTTTGA AGGGCAAGAG ACTGGTAAAA AAGAGACAAT CGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002038.2
CDS	30..2249
Translation	MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE

Target ORF information:

RefSeq Version	NM_002047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycyl-tRNA synthetase (GARS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002047.3

ORF Insert Sequence:

ATGCCCTCTC CGCGTCCAGT GCTGCTTAGA GGTGCTCGCG CCGCTCTGCT GCTGCTGCTG 
CCGCCCCGGC TCTTAGCCCG ACCCTCGCTC CTGCTCCGCC GGTCCCTCAG CGCGGCCTCC 
TGCCCCCCGA TCTCCTTGCC CGCCGCCGCC TCCCGGAGCA GCATGGACGG CGCGGGGGCT 
GAGGAGGTGC TGGCACCTCT GAGGCTAGCA GTGCGCCAGC AGGGAGATCT TGTGCGAAAA 
CTCAAAGAAG ATAAAGCACC CCAAGTAGAC GTAGACAAAG CAGTGGCTGA GCTCAAAGCC 
CGCAAGAGGG TTCTGGAAGC AAAGGAGCTG GCGTTACAGC CCAAAGATGA TATTGTAGAC 
CGAGCAAAAA TGGAAGATAC CCTGAAGAGG AGGTTTTTCT ATGATCAAGC TTTTGCTATT 
TATGGAGGTG TTAGTGGTCT GTATGACTTT GGGCCAGTTG GCTGTGCTTT GAAGAACAAT 
ATTATTCAGA CCTGGAGGCA GCACTTTATC CAAGAGGAAC AGATCCTGGA GATCGATTGC 
ACCATGCTCA CCCCTGAGCC AGTTTTAAAG ACCTCTGGCC ATGTAGACAA ATTTGCTGAC 
TTCATGGTGA AAGACGTAAA AAATGGAGAA TGTTTTCGTG CTGACCATCT ATTAAAAGCT 
CATTTACAGA AATTGATGTC TGATAAGAAG TGTTCTGTCG AAAAGAAATC AGAAATGGAA 
AGTGTTTTGG CCCAGCTTGA TAACTATGGA CAGCAAGAAC TTGCGGATCT TTTTGTGAAC 
TATAATGTAA AATCTCCCAT TACTGGAAAT GATCTATCCC CTCCAGTGTC TTTTAACTTA 
ATGTTCAAGA CTTTCATTGG GCCTGGAGGA AACATGCCTG GGTACTTGAG ACCAGAAACT 
GCACAGGGGA TTTTCTTGAA TTTCAAACGA CTTTTGGAGT TCAACCAAGG AAAGTTGCCT 
TTTGCTGCTG CCCAGATTGG AAATTCTTTT AGAAATGAGA TCTCCCCTCG ATCTGGACTG 
ATCAGAGTCA GAGAATTCAC AATGGCAGAA ATTGAGCACT TTGTAGATCC CAGTGAGAAA 
GACCACCCCA AGTTCCAGAA TGTGGCAGAC CTTCACCTTT ATTTGTATTC AGCAAAAGCC 
CAGGTCAGCG GACAGTCCGC TCGGAAAATG CGCCTGGGAG ATGCTGTTGA ACAGGGTGTG 
ATTAATAACA CAGTATTAGG CTATTTCATT GGCCGCATCT ACCTCTACCT CACGAAGGTT 
GGAATATCTC CAGATAAACT CCGCTTCCGG CAGCACATGG AGAATGAGAT GGCCCATTAT 
GCCTGTGACT GTTGGGATGC AGAATCCAAA ACATCCTACG GTTGGATTGA GATTGTTGGA 
TGTGCTGATC GTTCCTGTTA TGACCTCTCC TGTCATGCAC GAGCCACCAA AGTCCCACTT 
GTAGCTGAGA AACCTCTGAA AGAACCCAAA ACAGTCAATG TTGTTCAGTT TGAACCCAGT 
AAGGGAGCAA TTGGTAAGGC ATATAAGAAG GATGCAAAAC TGGTGATGGA GTATCTTGCC 
ATTTGTGATG AGTGCTACAT TACAGAAATG GAGATGCTGC TGAATGAGAA AGGGGAATTC 
ACAATTGAAA CTGAAGGGAA AACATTTCAG TTAACAAAAG ACATGATCAA TGTGAAGAGA 
TTCCAGAAAA CACTATATGT GGAAGAAGTT GTTCCGAATG TAATTGAACC TTCCTTCGGC 
CTGGGTAGGA TCATGTATAC GGTATTTGAA CATACATTCC ATGTACGAGA AGGAGATGAA 
CAGAGAACAT TCTTCAGTTT CCCTGCTGTA GTTGCTCCAT TCAAATGTTC CGTCCTCCCA 
CTGAGCCAAA ACCAGGAGTT CATGCCATTT GTCAAGGAAT TATCGGAAGC CCTGACCAGG 
CATGGAGTAT CTCACAAAGT AGACGATTCC TCTGGGTCAA TCGGAAGGCG CTATGCCAGG 
ACTGATGAGA TTGGCGTGGC TTTTGGTGTC ACCATTGACT TTGACACAGT GAACAAGACC 
CCCCACACTG CAACTCTGAG GGACCGTGAC TCAATGCGGC AGATAAGAGC AGAGATCTCT 
GAGCTGCCCA GCATAGTCCA AGACCTAGCC AATGGCAACA TCACATGGGC TGATGTGGAG 
GCCAGGTATC CTCTGTTTGA AGGGCAAGAG ACTGGTAAAA AAGAGACAAT CGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GARS1 ( NM_002047.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002038.2 Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25860
Clone ID Related Accession (Same CDS sequence)	NM_002047.3 , NM_002047.4
Accession Version	NM_002047.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	glycine--tRNA ligase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005154.1, DA386636.1, BC007755.2 and AA464978.1. On Nov 23, 2018 this sequence version replaced NM_002047.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.394868.1, AK074524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389266.8/ ENSP00000373918.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCTCTC CGCGTCCAGT GCTGCTTAGA GGTGCTCGCG CCGCTCTGCT GCTGCTGCTG 
CCGCCCCGGC TCTTAGCCCG ACCCTCGCTC CTGCTCCGCC GGTCCCTCAG CGCGGCCTCC 
TGCCCCCCGA TCTCCTTGCC CGCCGCCGCC TCCCGGAGCA GCATGGACGG CGCGGGGGCT 
GAGGAGGTGC TGGCACCTCT GAGGCTAGCA GTGCGCCAGC AGGGAGATCT TGTGCGAAAA 
CTCAAAGAAG ATAAAGCACC CCAAGTAGAC GTAGACAAAG CAGTGGCTGA GCTCAAAGCC 
CGCAAGAGGG TTCTGGAAGC AAAGGAGCTG GCGTTACAGC CCAAAGATGA TATTGTAGAC 
CGAGCAAAAA TGGAAGATAC CCTGAAGAGG AGGTTTTTCT ATGATCAAGC TTTTGCTATT 
TATGGAGGTG TTAGTGGTCT GTATGACTTT GGGCCAGTTG GCTGTGCTTT GAAGAACAAT 
ATTATTCAGA CCTGGAGGCA GCACTTTATC CAAGAGGAAC AGATCCTGGA GATCGATTGC 
ACCATGCTCA CCCCTGAGCC AGTTTTAAAG ACCTCTGGCC ATGTAGACAA ATTTGCTGAC 
TTCATGGTGA AAGACGTAAA AAATGGAGAA TGTTTTCGTG CTGACCATCT ATTAAAAGCT 
CATTTACAGA AATTGATGTC TGATAAGAAG TGTTCTGTCG AAAAGAAATC AGAAATGGAA 
AGTGTTTTGG CCCAGCTTGA TAACTATGGA CAGCAAGAAC TTGCGGATCT TTTTGTGAAC 
TATAATGTAA AATCTCCCAT TACTGGAAAT GATCTATCCC CTCCAGTGTC TTTTAACTTA 
ATGTTCAAGA CTTTCATTGG GCCTGGAGGA AACATGCCTG GGTACTTGAG ACCAGAAACT 
GCACAGGGGA TTTTCTTGAA TTTCAAACGA CTTTTGGAGT TCAACCAAGG AAAGTTGCCT 
TTTGCTGCTG CCCAGATTGG AAATTCTTTT AGAAATGAGA TCTCCCCTCG ATCTGGACTG 
ATCAGAGTCA GAGAATTCAC AATGGCAGAA ATTGAGCACT TTGTAGATCC CAGTGAGAAA 
GACCACCCCA AGTTCCAGAA TGTGGCAGAC CTTCACCTTT ATTTGTATTC AGCAAAAGCC 
CAGGTCAGCG GACAGTCCGC TCGGAAAATG CGCCTGGGAG ATGCTGTTGA ACAGGGTGTG 
ATTAATAACA CAGTATTAGG CTATTTCATT GGCCGCATCT ACCTCTACCT CACGAAGGTT 
GGAATATCTC CAGATAAACT CCGCTTCCGG CAGCACATGG AGAATGAGAT GGCCCATTAT 
GCCTGTGACT GTTGGGATGC AGAATCCAAA ACATCCTACG GTTGGATTGA GATTGTTGGA 
TGTGCTGATC GTTCCTGTTA TGACCTCTCC TGTCATGCAC GAGCCACCAA AGTCCCACTT 
GTAGCTGAGA AACCTCTGAA AGAACCCAAA ACAGTCAATG TTGTTCAGTT TGAACCCAGT 
AAGGGAGCAA TTGGTAAGGC ATATAAGAAG GATGCAAAAC TGGTGATGGA GTATCTTGCC 
ATTTGTGATG AGTGCTACAT TACAGAAATG GAGATGCTGC TGAATGAGAA AGGGGAATTC 
ACAATTGAAA CTGAAGGGAA AACATTTCAG TTAACAAAAG ACATGATCAA TGTGAAGAGA 
TTCCAGAAAA CACTATATGT GGAAGAAGTT GTTCCGAATG TAATTGAACC TTCCTTCGGC 
CTGGGTAGGA TCATGTATAC GGTATTTGAA CATACATTCC ATGTACGAGA AGGAGATGAA 
CAGAGAACAT TCTTCAGTTT CCCTGCTGTA GTTGCTCCAT TCAAATGTTC CGTCCTCCCA 
CTGAGCCAAA ACCAGGAGTT CATGCCATTT GTCAAGGAAT TATCGGAAGC CCTGACCAGG 
CATGGAGTAT CTCACAAAGT AGACGATTCC TCTGGGTCAA TCGGAAGGCG CTATGCCAGG 
ACTGATGAGA TTGGCGTGGC TTTTGGTGTC ACCATTGACT TTGACACAGT GAACAAGACC 
CCCCACACTG CAACTCTGAG GGACCGTGAC TCAATGCGGC AGATAAGAGC AGAGATCTCT 
GAGCTGCCCA GCATAGTCCA AGACCTAGCC AATGGCAACA TCACATGGGC TGATGTGGAG 
GCCAGGTATC CTCTGTTTGA AGGGCAAGAG ACTGGTAAAA AAGAGACAAT CGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002038.2
CDS	45..2264
Translation	MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE

Target ORF information:

RefSeq Version	NM_002047.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002047.4

ORF Insert Sequence:

ATGCCCTCTC CGCGTCCAGT GCTGCTTAGA GGTGCTCGCG CCGCTCTGCT GCTGCTGCTG 
CCGCCCCGGC TCTTAGCCCG ACCCTCGCTC CTGCTCCGCC GGTCCCTCAG CGCGGCCTCC 
TGCCCCCCGA TCTCCTTGCC CGCCGCCGCC TCCCGGAGCA GCATGGACGG CGCGGGGGCT 
GAGGAGGTGC TGGCACCTCT GAGGCTAGCA GTGCGCCAGC AGGGAGATCT TGTGCGAAAA 
CTCAAAGAAG ATAAAGCACC CCAAGTAGAC GTAGACAAAG CAGTGGCTGA GCTCAAAGCC 
CGCAAGAGGG TTCTGGAAGC AAAGGAGCTG GCGTTACAGC CCAAAGATGA TATTGTAGAC 
CGAGCAAAAA TGGAAGATAC CCTGAAGAGG AGGTTTTTCT ATGATCAAGC TTTTGCTATT 
TATGGAGGTG TTAGTGGTCT GTATGACTTT GGGCCAGTTG GCTGTGCTTT GAAGAACAAT 
ATTATTCAGA CCTGGAGGCA GCACTTTATC CAAGAGGAAC AGATCCTGGA GATCGATTGC 
ACCATGCTCA CCCCTGAGCC AGTTTTAAAG ACCTCTGGCC ATGTAGACAA ATTTGCTGAC 
TTCATGGTGA AAGACGTAAA AAATGGAGAA TGTTTTCGTG CTGACCATCT ATTAAAAGCT 
CATTTACAGA AATTGATGTC TGATAAGAAG TGTTCTGTCG AAAAGAAATC AGAAATGGAA 
AGTGTTTTGG CCCAGCTTGA TAACTATGGA CAGCAAGAAC TTGCGGATCT TTTTGTGAAC 
TATAATGTAA AATCTCCCAT TACTGGAAAT GATCTATCCC CTCCAGTGTC TTTTAACTTA 
ATGTTCAAGA CTTTCATTGG GCCTGGAGGA AACATGCCTG GGTACTTGAG ACCAGAAACT 
GCACAGGGGA TTTTCTTGAA TTTCAAACGA CTTTTGGAGT TCAACCAAGG AAAGTTGCCT 
TTTGCTGCTG CCCAGATTGG AAATTCTTTT AGAAATGAGA TCTCCCCTCG ATCTGGACTG 
ATCAGAGTCA GAGAATTCAC AATGGCAGAA ATTGAGCACT TTGTAGATCC CAGTGAGAAA 
GACCACCCCA AGTTCCAGAA TGTGGCAGAC CTTCACCTTT ATTTGTATTC AGCAAAAGCC 
CAGGTCAGCG GACAGTCCGC TCGGAAAATG CGCCTGGGAG ATGCTGTTGA ACAGGGTGTG 
ATTAATAACA CAGTATTAGG CTATTTCATT GGCCGCATCT ACCTCTACCT CACGAAGGTT 
GGAATATCTC CAGATAAACT CCGCTTCCGG CAGCACATGG AGAATGAGAT GGCCCATTAT 
GCCTGTGACT GTTGGGATGC AGAATCCAAA ACATCCTACG GTTGGATTGA GATTGTTGGA 
TGTGCTGATC GTTCCTGTTA TGACCTCTCC TGTCATGCAC GAGCCACCAA AGTCCCACTT 
GTAGCTGAGA AACCTCTGAA AGAACCCAAA ACAGTCAATG TTGTTCAGTT TGAACCCAGT 
AAGGGAGCAA TTGGTAAGGC ATATAAGAAG GATGCAAAAC TGGTGATGGA GTATCTTGCC 
ATTTGTGATG AGTGCTACAT TACAGAAATG GAGATGCTGC TGAATGAGAA AGGGGAATTC 
ACAATTGAAA CTGAAGGGAA AACATTTCAG TTAACAAAAG ACATGATCAA TGTGAAGAGA 
TTCCAGAAAA CACTATATGT GGAAGAAGTT GTTCCGAATG TAATTGAACC TTCCTTCGGC 
CTGGGTAGGA TCATGTATAC GGTATTTGAA CATACATTCC ATGTACGAGA AGGAGATGAA 
CAGAGAACAT TCTTCAGTTT CCCTGCTGTA GTTGCTCCAT TCAAATGTTC CGTCCTCCCA 
CTGAGCCAAA ACCAGGAGTT CATGCCATTT GTCAAGGAAT TATCGGAAGC CCTGACCAGG 
CATGGAGTAT CTCACAAAGT AGACGATTCC TCTGGGTCAA TCGGAAGGCG CTATGCCAGG 
ACTGATGAGA TTGGCGTGGC TTTTGGTGTC ACCATTGACT TTGACACAGT GAACAAGACC 
CCCCACACTG CAACTCTGAG GGACCGTGAC TCAATGCGGC AGATAAGAGC AGAGATCTCT 
GAGCTGCCCA GCATAGTCCA AGACCTAGCC AATGGCAACA TCACATGGGC TGATGTGGAG 
GCCAGGTATC CTCTGTTTGA AGGGCAAGAG ACTGGTAAAA AAGAGACAAT CGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compound heterozygosity for loss-of-function GARS variants results in a multisystem developmental syndrome that includes severe growth retardation.
Human mutation38(10)1412-1420(2017 Oct)
Oprescu SN,Chepa-Lotrea X,Takase R,Golas G,Markello TC,Adams DR,Toro C,Gropman AL,Hou YM,Malicdan MCV,Gahl WA,Tifft CJ,Antonellis A

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Scientific reports7(1)9216(2017 Aug)
Sleigh JN,G?mez-Mart?n A,Wei N,Bai G,Yang XL,Schiavo G

Crystal Structure of the Wild-Type Human GlyRS Bound with tRNA(Gly) in a Productive Conformation.
Journal of molecular biology428(18)3603-14(2016 Sep)
Qin X,Deng X,Chen L,Xie W

Neddylation requires glycyl-tRNA synthetase to protect activated E2.
Nature structural & molecular biology23(8)730-7(2016 08)
Mo Z,Zhang Q,Liu Z,Lauer J,Shi Y,Sun L,Griffin PR,Yang XL

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GARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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