APPL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APPL1
Entrez Gene ID	26060
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
Synonyms	APPL,DIP13alpha,MODY14
General protein information	Preferred Names adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 Names DCC-interacting protein 13-alpha AKT2 interactor adapter protein containing PH domain, PTB domain and leucine zipper motif 1 adaptor protein containing pH domain, PTB domain and leucine zipper motif 1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 dip13-alpha signaling adaptor protein DIP13alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p14.3
Summary	The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008].
Disorder MIM:	604299
Disorder Html:	{Maturity-onset diabetes of the young, type 14}, 616511 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011533583.3	XP_011531885.1	DCC-interacting protein 13-alpha isoform X1
NM_012096.3	NP_036228.1	DCC-interacting protein 13-alpha
NM_012096.2	NP_036228.1	DCC-interacting protein 13-alpha
XM_011533583.2	XP_011531885.1	DCC-interacting protein 13-alpha isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPL1	XP_001173707.1
Canis lupus familiaris (dog)	APPL1	XP_849075.1
Gallus gallus (chicken)	APPL1	XP_414344.4
Bos taurus (cattle)	APPL1	NP_001092473.1
Mus musculus (house mouse)	Appl1	NP_660256.1
Homo sapiens (human)	APPL1	NP_036228.1
Rattus norvegicus (Norway rat)	Appl1	XP_006252792.1
Danio rerio (zebrafish)	appl1	NP_001121546.1
Xenopus tropicalis (tropical clawed frog)	appl1	XP_004914231.1

Gene Ontology
Bibliography

Related articles in PubMed

APPL1 promotes the migration of gastric cancer cells by regulating Akt2 phosphorylation.
Liu Y, Zhang C, Zhao L, Du N, Hou N, Song T, Huang C
International journal of oncology51(4)1343-1351(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased expression of APPL1 is associated with polycystic ovary syndrome.
Title: Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients.

APPL1 positively mediated leptin signaling.
Title: APPL1-Mediating Leptin Signaling Contributes to Proliferation and Migration of Cancer Cells.

Data show that signal transducing adaptor proteins APPL1 and APPL2 are required for TGFbeta-induced nuclear translocation of TGFbeta type I receptor (TbetaRI)-ICD and for cancer cell invasiveness of prostate and breast cancer cell lines.
Title: APPL proteins promote TGF?-induced nuclear transport of the TGF? type I receptor intracellular domain.

Low expression of APPL1 is associated with metastasis in prostate cancer.
Title: Endosomal gene expression: a new indicator for prostate cancer patient prognosis?

article provides evidence that GG genotype and G carrier (CG+GG) genotypes of the rs4640525 polymorphism in the APPL1 gene may be suitable susceptibility biomarkers for NAFLD
Title: Association of APPL1 Gene Polymorphism with Non-Alcoholic Fatty Liver Disease Susceptibility in a Chinese Han Population.

The following APPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15816	NM_012096.3 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu66778	XM_011533583.3 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu15816	NM_012096.2	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu66778	XM_011533583.2	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL1 ( NM_012096.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036228.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Price & Availability↑

CloneID	OHu15816
Clone ID Related Accession (Same CDS sequence)	NM_012096.3 , NM_012096.2
Accession Version	NM_012096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093928.2, BC028599.2 and AF169797.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_012096.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.53167.1, SRR1660805.136161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000288266.8/ ENSP00000288266.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTAG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACCTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGTTTTC CATTGGGAGG TGATGATGAA 
GTTATGAGCT CTACATTGCA ACAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTGCTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATACT AACATTAAAG GAAGTATTTC AGATTGCAAG TAATGATCAT 
GATGCTGCGA TTAATAGATA TAGCCGTTTA TCAAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT GTACACATCC AGAAAGAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAACTGGAAG AATTTTTAGC TAATATTGGA ACAAGCGTTC AGAATGTTCG CAGGGAAATG 
GACAGTGATA TAGAGACCAT GCAACAGACA ATAGAGGATT TGGAAGTAGC CAGTGATCCC 
TTATATGTGC CTGACCCAGA CCCCACCAAA TTTCCTGTTA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAATTTAATG AGTCAGGCCC GTGGGGATGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAT 
TGTTTTCAGA TCACCTCTTT CGATGGAAAA AAATCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGATCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATACTTAAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTA AATCAATCAG CTCTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGCGGCCAG CAGCAGGACA ATCTCGGCCA 
CCGACAGCTC GAACCAGCAG TTCAGGATCC TTAGGATCTG AGTCTACAAA TTTGGCTGCC 
CTCTCTCTAG ATTCTCTTGT TGCCCCAGAC ACCCCAATAC AGTTTGACAT AATTTCTCCT 
GTGTGTGAAG ATCAGCCTGG CCAGGCAAAA GCCTTTGGCC AGGGAGGCAG GCGTACAAAT 
CCATTTGGAG AATCTGGAGG AAGTACAAAA TCTGAAACTG AAGATTCTAT TCTTCATCAG 
TTATTTATTG TCCGATTCCT TGGTTCAATG GAGGTGAAAT CAGATGACCA TCCAGATGTT 
GTTTATGAAA CTATGCGCCA AATCTTAGCT GCCCGGGCCA TCCATAACAT CTTTCGTATG 
ACAGAATCGC ATTTATTAGT CACTTGTGAC TGTTTAAAGT TAATTGATCC ACAGACACAA 
GTTACAAGGC TCACGTTTCC ATTACCTTGT GTAGTTTTGT ATGCTACACA CCAGGAAAAT 
AAGCGCCTTT TTGGATTTGT TCTTCGGACA TCAAGCGGGA GAAGTGAAAG TAATCTGTCA 
TCAGTCTGCT ATATATTTGA GTCAAACAAT GAGGGGGAAA AGATATGTGA TTCTGTTGGA 
CTGGCAAAAC AGATAGCTTT GCATGCTGAA CTGGATCGTA GGGCATCAGA AAAACAAAAA 
GAAATAGAGA GAGTAAAAGA GAAGCAACAG AAAGAACTCA ATAAACAAAA ACAGATTGAA 
AAGGACTTGG AAGAACAAAG TCGGTTGATA GCTGCTTCCA GTAGACCAAA CCAAGCCAGT 
AGTGAGGGGC AGTTTGTTGT CCTTAGCAGT AGCCAGTCAG AAGAGAGTGA TTTGGGAGAA 
GGAGGAAAGA AGAGAGAATC AGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036228.1
CDS	156..2285
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

Target ORF information:

RefSeq Version	NM_012096.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012096.3

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTAG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACCTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGTTTTC CATTGGGAGG TGATGATGAA 
GTTATGAGCT CTACATTGCA ACAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTGCTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATACT AACATTAAAG GAAGTATTTC AGATTGCAAG TAATGATCAT 
GATGCTGCGA TTAATAGATA TAGCCGTTTA TCAAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT GTACACATCC AGAAAGAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAACTGGAAG AATTTTTAGC TAATATTGGA ACAAGCGTTC AGAATGTTCG CAGGGAAATG 
GACAGTGATA TAGAGACCAT GCAACAGACA ATAGAGGATT TGGAAGTAGC CAGTGATCCC 
TTATATGTGC CTGACCCAGA CCCCACCAAA TTTCCTGTTA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAATTTAATG AGTCAGGCCC GTGGGGATGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAT 
TGTTTTCAGA TCACCTCTTT CGATGGAAAA AAATCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGATCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATACTTAAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTA AATCAATCAG CTCTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGCGGCCAG CAGCAGGACA ATCTCGGCCA 
CCGACAGCTC GAACCAGCAG TTCAGGATCC TTAGGATCTG AGTCTACAAA TTTGGCTGCC 
CTCTCTCTAG ATTCTCTTGT TGCCCCAGAC ACCCCAATAC AGTTTGACAT AATTTCTCCT 
GTGTGTGAAG ATCAGCCTGG CCAGGCAAAA GCCTTTGGCC AGGGAGGCAG GCGTACAAAT 
CCATTTGGAG AATCTGGAGG AAGTACAAAA TCTGAAACTG AAGATTCTAT TCTTCATCAG 
TTATTTATTG TCCGATTCCT TGGTTCAATG GAGGTGAAAT CAGATGACCA TCCAGATGTT 
GTTTATGAAA CTATGCGCCA AATCTTAGCT GCCCGGGCCA TCCATAACAT CTTTCGTATG 
ACAGAATCGC ATTTATTAGT CACTTGTGAC TGTTTAAAGT TAATTGATCC ACAGACACAA 
GTTACAAGGC TCACGTTTCC ATTACCTTGT GTAGTTTTGT ATGCTACACA CCAGGAAAAT 
AAGCGCCTTT TTGGATTTGT TCTTCGGACA TCAAGCGGGA GAAGTGAAAG TAATCTGTCA 
TCAGTCTGCT ATATATTTGA GTCAAACAAT GAGGGGGAAA AGATATGTGA TTCTGTTGGA 
CTGGCAAAAC AGATAGCTTT GCATGCTGAA CTGGATCGTA GGGCATCAGA AAAACAAAAA 
GAAATAGAGA GAGTAAAAGA GAAGCAACAG AAAGAACTCA ATAAACAAAA ACAGATTGAA 
AAGGACTTGG AAGAACAAAG TCGGTTGATA GCTGCTTCCA GTAGACCAAA CCAAGCCAGT 
AGTGAGGGGC AGTTTGTTGT CCTTAGCAGT AGCCAGTCAG AAGAGAGTGA TTTGGGAGAA 
GGAGGAAAGA AGAGAGAATC AGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( NM_012096.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036228.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Price & Availability↑

CloneID	OHu15816
Clone ID Related Accession (Same CDS sequence)	NM_012096.3 , NM_012096.2
Accession Version	NM_012096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028599.2, AF169797.1 and AC093928.2. This sequence is a reference standard in the RefSeqGene project. On Feb 6, 2007 this sequence version replaced NM_012096.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028599.2, SRR1660803.53167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTAG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACCTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGTTTTC CATTGGGAGG TGATGATGAA 
GTTATGAGCT CTACATTGCA ACAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTGCTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATACT AACATTAAAG GAAGTATTTC AGATTGCAAG TAATGATCAT 
GATGCTGCGA TTAATAGATA TAGCCGTTTA TCAAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT GTACACATCC AGAAAGAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAACTGGAAG AATTTTTAGC TAATATTGGA ACAAGCGTTC AGAATGTTCG CAGGGAAATG 
GACAGTGATA TAGAGACCAT GCAACAGACA ATAGAGGATT TGGAAGTAGC CAGTGATCCC 
TTATATGTGC CTGACCCAGA CCCCACCAAA TTTCCTGTTA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAATTTAATG AGTCAGGCCC GTGGGGATGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAT 
TGTTTTCAGA TCACCTCTTT CGATGGAAAA AAATCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGATCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATACTTAAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTA AATCAATCAG CTCTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGCGGCCAG CAGCAGGACA ATCTCGGCCA 
CCGACAGCTC GAACCAGCAG TTCAGGATCC TTAGGATCTG AGTCTACAAA TTTGGCTGCC 
CTCTCTCTAG ATTCTCTTGT TGCCCCAGAC ACCCCAATAC AGTTTGACAT AATTTCTCCT 
GTGTGTGAAG ATCAGCCTGG CCAGGCAAAA GCCTTTGGCC AGGGAGGCAG GCGTACAAAT 
CCATTTGGAG AATCTGGAGG AAGTACAAAA TCTGAAACTG AAGATTCTAT TCTTCATCAG 
TTATTTATTG TCCGATTCCT TGGTTCAATG GAGGTGAAAT CAGATGACCA TCCAGATGTT 
GTTTATGAAA CTATGCGCCA AATCTTAGCT GCCCGGGCCA TCCATAACAT CTTTCGTATG 
ACAGAATCGC ATTTATTAGT CACTTGTGAC TGTTTAAAGT TAATTGATCC ACAGACACAA 
GTTACAAGGC TCACGTTTCC ATTACCTTGT GTAGTTTTGT ATGCTACACA CCAGGAAAAT 
AAGCGCCTTT TTGGATTTGT TCTTCGGACA TCAAGCGGGA GAAGTGAAAG TAATCTGTCA 
TCAGTCTGCT ATATATTTGA GTCAAACAAT GAGGGGGAAA AGATATGTGA TTCTGTTGGA 
CTGGCAAAAC AGATAGCTTT GCATGCTGAA CTGGATCGTA GGGCATCAGA AAAACAAAAA 
GAAATAGAGA GAGTAAAAGA GAAGCAACAG AAAGAACTCA ATAAACAAAA ACAGATTGAA 
AAGGACTTGG AAGAACAAAG TCGGTTGATA GCTGCTTCCA GTAGACCAAA CCAAGCCAGT 
AGTGAGGGGC AGTTTGTTGT CCTTAGCAGT AGCCAGTCAG AAGAGAGTGA TTTGGGAGAA 
GGAGGAAAGA AGAGAGAATC AGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036228.1
CDS	148..2277
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

Target ORF information:

RefSeq Version	NM_012096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012096.2

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTAG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACCTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGTTTTC CATTGGGAGG TGATGATGAA 
GTTATGAGCT CTACATTGCA ACAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTGCTTT CAACTCAACT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATACT AACATTAAAG GAAGTATTTC AGATTGCAAG TAATGATCAT 
GATGCTGCGA TTAATAGATA TAGCCGTTTA TCAAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT GTACACATCC AGAAAGAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAATAC TCTTCAGTAC AAGAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAACTGGAAG AATTTTTAGC TAATATTGGA ACAAGCGTTC AGAATGTTCG CAGGGAAATG 
GACAGTGATA TAGAGACCAT GCAACAGACA ATAGAGGATT TGGAAGTAGC CAGTGATCCC 
TTATATGTGC CTGACCCAGA CCCCACCAAA TTTCCTGTTA ATCGAAATTT AACCCGAAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAATTTAATG AGTCAGGCCC GTGGGGATGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGTTCAGTG ATGGCTGTGG ACTGTGAAGA CAGACGATAT 
TGTTTTCAGA TCACCTCTTT CGATGGAAAA AAATCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGATCATG AAGAGTGGAT CTGTACAATA AACAACATAT CTAAACAAAT ATACTTAAGT 
GAAAATCCAG AGGAAACTGC TGCACGAGTA AATCAATCAG CTCTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGCGGCCAG CAGCAGGACA ATCTCGGCCA 
CCGACAGCTC GAACCAGCAG TTCAGGATCC TTAGGATCTG AGTCTACAAA TTTGGCTGCC 
CTCTCTCTAG ATTCTCTTGT TGCCCCAGAC ACCCCAATAC AGTTTGACAT AATTTCTCCT 
GTGTGTGAAG ATCAGCCTGG CCAGGCAAAA GCCTTTGGCC AGGGAGGCAG GCGTACAAAT 
CCATTTGGAG AATCTGGAGG AAGTACAAAA TCTGAAACTG AAGATTCTAT TCTTCATCAG 
TTATTTATTG TCCGATTCCT TGGTTCAATG GAGGTGAAAT CAGATGACCA TCCAGATGTT 
GTTTATGAAA CTATGCGCCA AATCTTAGCT GCCCGGGCCA TCCATAACAT CTTTCGTATG 
ACAGAATCGC ATTTATTAGT CACTTGTGAC TGTTTAAAGT TAATTGATCC ACAGACACAA 
GTTACAAGGC TCACGTTTCC ATTACCTTGT GTAGTTTTGT ATGCTACACA CCAGGAAAAT 
AAGCGCCTTT TTGGATTTGT TCTTCGGACA TCAAGCGGGA GAAGTGAAAG TAATCTGTCA 
TCAGTCTGCT ATATATTTGA GTCAAACAAT GAGGGGGAAA AGATATGTGA TTCTGTTGGA 
CTGGCAAAAC AGATAGCTTT GCATGCTGAA CTGGATCGTA GGGCATCAGA AAAACAAAAA 
GAAATAGAGA GAGTAAAAGA GAAGCAACAG AAAGAACTCA ATAAACAAAA ACAGATTGAA 
AAGGACTTGG AAGAACAAAG TCGGTTGATA GCTGCTTCCA GTAGACCAAA CCAAGCCAGT 
AGTGAGGGGC AGTTTGTTGT CCTTAGCAGT AGCCAGTCAG AAGAGAGTGA TTTGGGAGAA 
GGAGGAAAGA AGAGAGAATC AGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_011533583.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531885.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

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CloneID	OHu66778
Clone ID Related Accession (Same CDS sequence)	XM_011533583.3 , XM_011533583.2
Accession Version	XM_011533583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGT CTTTACTAGG TGTATTTGAA GAAGATGCCA CAGCTATTTC CAACTATATG 
AACCAGTTGT ATCAAGCTAT GCATCGGATT TATGATGCAC AGAATGAATT AAGTGCAGCA 
ACACACCTGA CCTCAAAACT TTTAAAAGAA TATGAAAAAC AGCGTTTTCC ATTGGGAGGT 
GATGATGAAG TTATGAGCTC TACATTGCAA CAGTTTTCAA AAGTTATAGA TGAGCTTAGC 
TCTTGTCATG CAGTGCTTTC AACTCAACTT GCTGATGCCA TGATGTTCCC CATTACCCAG 
TTTAAAGAAA GAGATCTGAA AGAAATACTA ACATTAAAGG AAGTATTTCA GATTGCAAGT 
AATGATCATG ATGCTGCGAT TAATAGATAT AGCCGTTTAT CAAAAAAAAG AGAAAATGAC 
AAGGTGAAGT ATGAAGTAAC AGAAGATGTG TACACATCCA GAAAGAAACA ACACCAGACC 
ATGATGCATT ATTTTTGTGC ATTAAATACT CTTCAGTACA AGAAGAAAAT AGCATTGTTA 
GAACCTCTAC TTGGGTACAT GCAAGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTTAATGAAC AACTGGAAGA ATTTTTAGCT AATATTGGAA CAAGCGTTCA GAATGTTCGC 
AGGGAAATGG ACAGTGATAT AGAGACCATG CAACAGACAA TAGAGGATTT GGAAGTAGCC 
AGTGATCCCT TATATGTGCC TGACCCAGAC CCCACCAAAT TTCCTGTTAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT TAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GACAGACAGT TTTACTTCAC GCAGGGTGGA AATTTAATGA GTCAGGCCCG TGGGGATGTA 
GCAGGAGGCC TGGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATATT GTTTTCAGAT CACCTCTTTC GATGGAAAAA AATCTTCAAT TTTGCAAGCA 
GAGAGTAAAA AAGATCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TACTTAAGTG AAAATCCAGA GGAAACTGCT GCACGAGTAA ATCAATCAGC TCTGGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGG CACGAGAGCC TGCGGCCAGC AGCAGGACAA 
TCTCGGCCAC CGACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCTACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGACA CCCCAATACA GTTTGACATA 
ATTTCTCCTG TGTGTGAAGA TCAGCCTGGC CAGGCAAAAG CCTTTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA AGTACAAAAT CTGAAACTGA AGATTCTATT 
CTTCATCAGT TATTTATTGT CCGATTCCTT GGTTCAATGG AGGTGAAATC AGATGACCAT 
CCAGATGTTG TTTATGAAAC TATGCGCCAA ATCTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCGCA TTTATTAGTC ACTTGTGACT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCTTGTG TAGTTTTGTA TGCTACACAC 
CAGGAAAATA AGCGCCTTTT TGGATTTGTT CTTCGGACAT CAAGCGGGAG AAGTGAAAGT 
AATCTGTCAT CAGTCTGCTA TATATTTGAG TCAAACAATG AGGGGGAAAA GATATGTGAT 
TCTGTTGGAC TGGCAAAACA GATAGCTTTG CATGCTGAAC TGGATCGTAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAA TAAACAAAAA 
CAGATTGAAA AGGACTTGGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GTGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG GAGGAAAGAA GAGAGAATCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531885.1
CDS	252..2330
Translation	MTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

Target ORF information:

RefSeq Version	XM_011533583.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533583.3

ORF Insert Sequence:

ATGACAAGGT CTTTACTAGG TGTATTTGAA GAAGATGCCA CAGCTATTTC CAACTATATG 
AACCAGTTGT ATCAAGCTAT GCATCGGATT TATGATGCAC AGAATGAATT AAGTGCAGCA 
ACACACCTGA CCTCAAAACT TTTAAAAGAA TATGAAAAAC AGCGTTTTCC ATTGGGAGGT 
GATGATGAAG TTATGAGCTC TACATTGCAA CAGTTTTCAA AAGTTATAGA TGAGCTTAGC 
TCTTGTCATG CAGTGCTTTC AACTCAACTT GCTGATGCCA TGATGTTCCC CATTACCCAG 
TTTAAAGAAA GAGATCTGAA AGAAATACTA ACATTAAAGG AAGTATTTCA GATTGCAAGT 
AATGATCATG ATGCTGCGAT TAATAGATAT AGCCGTTTAT CAAAAAAAAG AGAAAATGAC 
AAGGTGAAGT ATGAAGTAAC AGAAGATGTG TACACATCCA GAAAGAAACA ACACCAGACC 
ATGATGCATT ATTTTTGTGC ATTAAATACT CTTCAGTACA AGAAGAAAAT AGCATTGTTA 
GAACCTCTAC TTGGGTACAT GCAAGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTTAATGAAC AACTGGAAGA ATTTTTAGCT AATATTGGAA CAAGCGTTCA GAATGTTCGC 
AGGGAAATGG ACAGTGATAT AGAGACCATG CAACAGACAA TAGAGGATTT GGAAGTAGCC 
AGTGATCCCT TATATGTGCC TGACCCAGAC CCCACCAAAT TTCCTGTTAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT TAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GACAGACAGT TTTACTTCAC GCAGGGTGGA AATTTAATGA GTCAGGCCCG TGGGGATGTA 
GCAGGAGGCC TGGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATATT GTTTTCAGAT CACCTCTTTC GATGGAAAAA AATCTTCAAT TTTGCAAGCA 
GAGAGTAAAA AAGATCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TACTTAAGTG AAAATCCAGA GGAAACTGCT GCACGAGTAA ATCAATCAGC TCTGGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGG CACGAGAGCC TGCGGCCAGC AGCAGGACAA 
TCTCGGCCAC CGACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCTACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGACA CCCCAATACA GTTTGACATA 
ATTTCTCCTG TGTGTGAAGA TCAGCCTGGC CAGGCAAAAG CCTTTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA AGTACAAAAT CTGAAACTGA AGATTCTATT 
CTTCATCAGT TATTTATTGT CCGATTCCTT GGTTCAATGG AGGTGAAATC AGATGACCAT 
CCAGATGTTG TTTATGAAAC TATGCGCCAA ATCTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCGCA TTTATTAGTC ACTTGTGACT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCTTGTG TAGTTTTGTA TGCTACACAC 
CAGGAAAATA AGCGCCTTTT TGGATTTGTT CTTCGGACAT CAAGCGGGAG AAGTGAAAGT 
AATCTGTCAT CAGTCTGCTA TATATTTGAG TCAAACAATG AGGGGGAAAA GATATGTGAT 
TCTGTTGGAC TGGCAAAACA GATAGCTTTG CATGCTGAAC TGGATCGTAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAA TAAACAAAAA 
CAGATTGAAA AGGACTTGGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GTGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG GAGGAAAGAA GAGAGAATCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_011533583.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531885.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66778
Clone ID Related Accession (Same CDS sequence)	XM_011533583.3 , XM_011533583.2
Accession Version	XM_011533583.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGT CTTTACTAGG TGTATTTGAA GAAGATGCCA CAGCTATTTC CAACTATATG 
AACCAGTTGT ATCAAGCTAT GCATCGGATT TATGATGCAC AGAATGAATT AAGTGCAGCA 
ACACACCTGA CCTCAAAACT TTTAAAAGAA TATGAAAAAC AGCGTTTTCC ATTGGGAGGT 
GATGATGAAG TTATGAGCTC TACATTGCAA CAGTTTTCAA AAGTTATAGA TGAGCTTAGC 
TCTTGTCATG CAGTGCTTTC AACTCAACTT GCTGATGCCA TGATGTTCCC CATTACCCAG 
TTTAAAGAAA GAGATCTGAA AGAAATACTA ACATTAAAGG AAGTATTTCA GATTGCAAGT 
AATGATCATG ATGCTGCGAT TAATAGATAT AGCCGTTTAT CAAAAAAAAG AGAAAATGAC 
AAGGTGAAGT ATGAAGTAAC AGAAGATGTG TACACATCCA GAAAGAAACA ACACCAGACC 
ATGATGCATT ATTTTTGTGC ATTAAATACT CTTCAGTACA AGAAGAAAAT AGCATTGTTA 
GAACCTCTAC TTGGGTACAT GCAAGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTTAATGAAC AACTGGAAGA ATTTTTAGCT AATATTGGAA CAAGCGTTCA GAATGTTCGC 
AGGGAAATGG ACAGTGATAT AGAGACCATG CAACAGACAA TAGAGGATTT GGAAGTAGCC 
AGTGATCCCT TATATGTGCC TGACCCAGAC CCCACCAAAT TTCCTGTTAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT TAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GACAGACAGT TTTACTTCAC GCAGGGTGGA AATTTAATGA GTCAGGCCCG TGGGGATGTA 
GCAGGAGGCC TGGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATATT GTTTTCAGAT CACCTCTTTC GATGGAAAAA AATCTTCAAT TTTGCAAGCA 
GAGAGTAAAA AAGATCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TACTTAAGTG AAAATCCAGA GGAAACTGCT GCACGAGTAA ATCAATCAGC TCTGGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGG CACGAGAGCC TGCGGCCAGC AGCAGGACAA 
TCTCGGCCAC CGACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCTACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGACA CCCCAATACA GTTTGACATA 
ATTTCTCCTG TGTGTGAAGA TCAGCCTGGC CAGGCAAAAG CCTTTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA AGTACAAAAT CTGAAACTGA AGATTCTATT 
CTTCATCAGT TATTTATTGT CCGATTCCTT GGTTCAATGG AGGTGAAATC AGATGACCAT 
CCAGATGTTG TTTATGAAAC TATGCGCCAA ATCTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCGCA TTTATTAGTC ACTTGTGACT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCTTGTG TAGTTTTGTA TGCTACACAC 
CAGGAAAATA AGCGCCTTTT TGGATTTGTT CTTCGGACAT CAAGCGGGAG AAGTGAAAGT 
AATCTGTCAT CAGTCTGCTA TATATTTGAG TCAAACAATG AGGGGGAAAA GATATGTGAT 
TCTGTTGGAC TGGCAAAACA GATAGCTTTG CATGCTGAAC TGGATCGTAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAA TAAACAAAAA 
CAGATTGAAA AGGACTTGGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GTGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG GAGGAAAGAA GAGAGAATCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531885.1
CDS	252..2330
Translation	MTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

Target ORF information:

RefSeq Version	XM_011533583.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533583.2

ORF Insert Sequence:

ATGACAAGGT CTTTACTAGG TGTATTTGAA GAAGATGCCA CAGCTATTTC CAACTATATG 
AACCAGTTGT ATCAAGCTAT GCATCGGATT TATGATGCAC AGAATGAATT AAGTGCAGCA 
ACACACCTGA CCTCAAAACT TTTAAAAGAA TATGAAAAAC AGCGTTTTCC ATTGGGAGGT 
GATGATGAAG TTATGAGCTC TACATTGCAA CAGTTTTCAA AAGTTATAGA TGAGCTTAGC 
TCTTGTCATG CAGTGCTTTC AACTCAACTT GCTGATGCCA TGATGTTCCC CATTACCCAG 
TTTAAAGAAA GAGATCTGAA AGAAATACTA ACATTAAAGG AAGTATTTCA GATTGCAAGT 
AATGATCATG ATGCTGCGAT TAATAGATAT AGCCGTTTAT CAAAAAAAAG AGAAAATGAC 
AAGGTGAAGT ATGAAGTAAC AGAAGATGTG TACACATCCA GAAAGAAACA ACACCAGACC 
ATGATGCATT ATTTTTGTGC ATTAAATACT CTTCAGTACA AGAAGAAAAT AGCATTGTTA 
GAACCTCTAC TTGGGTACAT GCAAGCTCAG ATAAGTTTCT TTAAGATGGG TTCTGAAAAT 
CTTAATGAAC AACTGGAAGA ATTTTTAGCT AATATTGGAA CAAGCGTTCA GAATGTTCGC 
AGGGAAATGG ACAGTGATAT AGAGACCATG CAACAGACAA TAGAGGATTT GGAAGTAGCC 
AGTGATCCCT TATATGTGCC TGACCCAGAC CCCACCAAAT TTCCTGTTAA TCGAAATTTA 
ACCCGAAAGG CTGGATACCT TAATGCTAGG AATAAAACAG GCTTGGTGTC ATCTACCTGG 
GACAGACAGT TTTACTTCAC GCAGGGTGGA AATTTAATGA GTCAGGCCCG TGGGGATGTA 
GCAGGAGGCC TGGCCATGGA CATAGACAAC TGTTCAGTGA TGGCTGTGGA CTGTGAAGAC 
AGACGATATT GTTTTCAGAT CACCTCTTTC GATGGAAAAA AATCTTCAAT TTTGCAAGCA 
GAGAGTAAAA AAGATCATGA AGAGTGGATC TGTACAATAA ACAACATATC TAAACAAATA 
TACTTAAGTG AAAATCCAGA GGAAACTGCT GCACGAGTAA ATCAATCAGC TCTGGAAGCT 
GTCACTCCTT CCCCATCTTT CCAGCAGAGG CACGAGAGCC TGCGGCCAGC AGCAGGACAA 
TCTCGGCCAC CGACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCTACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGACA CCCCAATACA GTTTGACATA 
ATTTCTCCTG TGTGTGAAGA TCAGCCTGGC CAGGCAAAAG CCTTTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA AGTACAAAAT CTGAAACTGA AGATTCTATT 
CTTCATCAGT TATTTATTGT CCGATTCCTT GGTTCAATGG AGGTGAAATC AGATGACCAT 
CCAGATGTTG TTTATGAAAC TATGCGCCAA ATCTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCGCA TTTATTAGTC ACTTGTGACT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCTTGTG TAGTTTTGTA TGCTACACAC 
CAGGAAAATA AGCGCCTTTT TGGATTTGTT CTTCGGACAT CAAGCGGGAG AAGTGAAAGT 
AATCTGTCAT CAGTCTGCTA TATATTTGAG TCAAACAATG AGGGGGAAAA GATATGTGAT 
TCTGTTGGAC TGGCAAAACA GATAGCTTTG CATGCTGAAC TGGATCGTAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAA TAAACAAAAA 
CAGATTGAAA AGGACTTGGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GTGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG GAGGAAAGAA GAGAGAATCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APPL1 promotes the migration of gastric cancer cells by regulating Akt2 phosphorylation.
International journal of oncology51(4)1343-1351(2017 Oct)
Liu Y,Zhang C,Zhao L,Du N,Hou N,Song T,Huang C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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APPL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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