RTTN gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011525908.2	XP_011524210.1	rotatin isoform X13
XM_011525902.2	XP_011524204.1	rotatin isoform X2
XM_024451139.1	XP_024306907.1	rotatin isoform X8
XM_011525906.2	XP_011524208.1	rotatin isoform X11
XM_011525903.2	XP_011524205.1	rotatin isoform X4
XM_017025694.1	XP_016881183.1	rotatin isoform X6
XM_024451140.1	XP_024306908.1	rotatin isoform X8
XM_017025696.1	XP_016881185.1	rotatin isoform X13
NM_001318520.1	NP_001305449.1	rotatin isoform 2
XM_011525904.2	XP_011524206.1	rotatin isoform X7
XM_006722434.3	XP_006722497.1	rotatin isoform X1
XM_006722435.3	XP_006722498.1	rotatin isoform X5
XM_011525907.2	XP_011524209.1	rotatin isoform X12
XM_011525904.3	XP_011524206.1	rotatin isoform X7
XM_017025695.1	XP_016881184.1	rotatin isoform X9
XM_011525908.3	XP_011524210.1	rotatin isoform X14
NM_173630.4	NP_775901.3	rotatin isoform 1
NM_173630.3	NP_775901.3	rotatin isoform 1
XM_017025693.1	XP_016881182.1	rotatin isoform X3
XM_011525905.2	XP_011524207.1	rotatin isoform X10
NM_001318520.2	NP_001305449.1	rotatin isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu87844	XM_017025696.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$811.30 $1159.00
OHu51155	NM_001318520.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu104535	XM_024451139.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104535	XM_024451140.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87843	XM_017025695.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu66632	XM_011525904.3 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66633	XM_011525905.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66634	XM_011525906.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66635	XM_011525907.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu66636	XM_011525908.3 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu87841	XM_017025693.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu87842	XM_017025694.1 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu19540	NM_173630.4 Latest version!	Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu51153	XM_006722434.3 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu51154	XM_006722435.3 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu66630	XM_011525902.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu66631	XM_011525903.2 Latest version!	Homo sapiens rotatin (RTTN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu66632	XM_011525904.2	Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66636	XM_011525908.2	Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu19540	NM_173630.3	Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu51155	NM_001318520.1	Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

RTTN ( XM_017025696.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881185.1 Homo sapiens rotatin (RTTN), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu87844
Clone ID Related Accession (Same CDS sequence)	XM_017025696.1
Accession Version	XM_017025696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAG CATTGACCTG GAACAAGTTT ATTGAATCTC TCTGTCCTGT CATCCCAATA 
CTACAGGGCT ATGCCGACAC AGAAGATCCT CTGGGTAACT GCATTCTCCT TCTAAGCAAA 
GCAAGTTCTG ACACAGAAGA GATGCTTCCG TGTACTACCC GATTAAAGTC CATGCTGCGA 
CTTTTGCTAG TGAAAAAGCC ATCGGTCCGT TCGCTAGCAT TAAAGCTTTT AGCATTCCAT 
CTTACCAGTG AAGAAGGGGC TGATACAAAG CGTCCTCTAA TAGACGCCAG AGTTCTCTCC 
AGAGTTACTG ATTTATTCAT TGGGAAGAAA CCTATTGAAC TCAGACTGGA TGACAGAAGA 
GAGCTGGTTA TTAAGTTGGA GACTGTTGAA AAGGTATATG AAATCTTCAC CTCAGATGAT 
GTTGATCTCG TTTTGAGAAA GTCAGCTGCT GAACAGTTAG CTGTGATTAT GCAAGATATT 
AAAATGCATG CTGTGGTGAA AAAGTTATGC TTAATTGACA AAATAATTGA GTATTTAAAT 
GAATGTGTGA GTCAAGATGG CAAGGTTGTA GAATGTTTGG TACAACCATG CCTCACACTC 
TTGAGGAAGG TTTTATGTGG TGATCCAGTC ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT 
CTTTTGACCG TGTTATTCAG AGTTTCCTTA ATATTTCATG AAGATTGTAG TGTCGTGACT 
GAAGTTGGAG CACTATTTTG TCTTCTATTA TTTGATGAAG TATCGAGAAT GGATATGTGG 
TCTGTTAATC CTTCCAATAA ACCTTCTTTG CCATCGGTCT TCAGTTTGCC TGTTTCCGTT 
TTTAGAAGGT ACCATCTACC TGTTCATGTA ATTGGACACC ATGCTGTGAG TCCTTACTCC 
ATAGTTTTGC CCTTATCTGC TGATTGTTTG GCCTTGAAGC CGGTGTCAGA TATGCTGAGA 
ATAGCTTGGA ACCTGTCATG GTATCATGGG AGTGATAATC TGTTGAAGCA AATGAACTCT 
GAAACTAAAA CGCAAGAAAT TTTAGATGCA TTGAAGTTAT CTACAGAGGA CATCCTCACT 
CTGAAGATAA CACACATGGC TAGTGGGCTG CAGGACTGCC TCCATTCCAT TGTTCAGGCT 
GCAACCCACA GGGAAGTTAG GGCTGCTGTC ACCAGGATGA GTTTTTATCT TCTGAATGAC 
AGATTGTCTT TAAAGGGTTG CCCTGGTCCA TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG 
CACACCGCAC TAAACAGGTT TTTACAGGTG CTTCCTGCTT GCACTGAAGA TGAGAAACTG 
CTAATAGATA TCATACATTT TTTAAATAAA TTAATAAAGG AACAAAGAAA AAATTCTTCA 
CTAGAACTTC TAAACTGGAT TCTCGAATTA CTTCTTAGAC ATAGTGCAAA CCCACTGTTA 
GACCTCTTGG TTCTGACAGA GTCACAGGCA CGAGAAGAAA CAGATGATAT CCGGACTGCT 
GTCAGGCAAC AACTTCAGAA AGAACTGATT GCTCTTTTTG ATACCTTGCT GCTCAATTTC 
ATGGAAGTTA CTGACAGGAA ATGCTCGGAA CTTCTTTACG TTTTTCAAAC GCAGCTGGCT 
CTGAAACTGC TCCAGTGTCT GAAAGTGACG GATGCGCCTC ATTTCTATGG CCTGCCGTCC 
CTTGAGCGGA CCTTACGAGG GATGGCTAAC CTCACTGCGT TTCCGGGATG GAGCTCACAC 
TCTCCTCTCA CAAAGCCTCT AGATATCTGT GTGAAGTACT TGTCAGGTCT CCTTGAGGTC 
ATTACTTCTT TTTATGTGGA GCGTGGAGGA AATGCTATGT CCTTCATGGG AAAAGGTGTT 
ACAAAGAGCA CAATTCTTTG CTTGCTTCAC TTATCCCATG AGATGATGGC CCAGGCTGGG 
AGCTTGGAGT GGATGTCACT TTGGTTCTTG CCTTTGGGTA GTCATAGTGA AGAACATATT 
CCTACTCAAC AAGGATTGGC TTGGTTGATT CCATTATGGG TTGATCGGGA CCCAGAGGTG 
AGATTCACTT CACTGGGATT AGGATCAGCA CTGACCACCC TTGAAACGGG CTGTGTGGCC 
TTAGCAAACA GTTGTCAGAA CATTTCCGGT GGGCTCTGGG GAACTGTGGT GAACATTCTT 
CTGGACCAGT CAGAATGTAG TATGGTGCGC CGGGAGGCGG CATTTATTCT TCAGAATCTC 
CTTGTAATTC CAATGCCTAC AGAAATTATA AAGGATTATA CTTGGCAGGG TCCCTGTGTT 
CATGATGAGG ACTCTGGCCT ATCGCTCATT GGAAAACCTG CCCTTCAGGC TCTTTTATAT 
CACTGCCATT TTTATGAACA TTTGAATCAG ATGGTAAAGC ATTGTTACCT AGGACGGTGT 
ATGTTTGATT TGAATTTTTC TGCTTTTGAT AGAAATTCAG AAAGCAATGA TTTAAATGGT 
TTAGATGACT CATTCAAGTT TTGGAGGGCT CCATCTAGGA CAAGTCAGGA TCGAGATCCA 
AGTTCTCTCT CCACCTCAGA AACAACGGTG GCACCTTCAT TGGGGAGTAC TGAATTTCAG 
CCACTTGTGC AGTCAACAAC ACTTCTACCT GAAGCCTCCC ATGACCAGTT TGTGGCTCAA 
GGTCACCAGG AAAGTACATC ACCACGGCCA CCTCATGATT CATCTCTTTC TGCTCCCCTG 
CCCAAACTGT GTGTTTTTGT TACTCCATCT CTTCTTTCAG CAATGTGCAG CCTCTTGGAC 
AACCTCTTGA CGATTGCTCC CAGAGACACT GCAAAGGCTT TTCGACAAGC TCATCTCATA 
GAACTTCTCT GTAGCATTGC AGATGCTACC CTCATACAGA CATGTGTCCA GGAACTCAGA 
GCCCTGCTGC CTTCATCACC TCCAGCTGAA CACACTCAGG CTCAGGTTTC CTTTCTCCTG 
GAATACCTAT CCTCTTTGTC CAGGCTTCTG CAGTCATGTT TATTGGTGGA GCCTGACCTT 
GTGATTCAGG ATGAGCTTGT GAAACCTCTT ATCACCAATA TCATTGGAAT TCTCACCATA 
TGTACCAAAG ATGTATTAGA TAAAGAGTTA ATATCAGCTT TTTATCACAC ATGGACACAT 
TTATTTAATC TTCTGGCCAT GCTCCTGAGG AAAGCTGGTG CCATCACACT CCCGTTTGTT 
ACCGTGGCCC TGGCCAAGCA CTGGACAGCG GCGATTGATA TGTTCTGCAC ATGTGCAGGC 
TTGTCTGCCA CGTGTCCTGC CCTGTATACT GCCAGCTTGC AATTCCTTTC TGTTCTCTTG 
ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT AAGAGCAAAA CACATTTATG CTGTAGTCCA 
ACAGTGGCTT CACTTCTTGA TGACTCTCAG GAAAATCAGA AATCTCTAGA ACAACTTAGT 
GATGTAATCC TTCAGTGCTA TGAAGGGAAA TCCTCCAAAG ATATCCTGAA AAGAGTAGCT 
GCAAATGCAT TGATGTCACT GCTGGCTGTC AGTAGAAGAG CACAGAAACA TGCTTTGAAA 
GCCAATCTTA TAGACAATTG CATGGAGCAG ATGAAACACA TAAATGCACA ACTGAACCTA 
GATTCTCTGA GGCCTGGGAA AGCAGCATTG AAAAAAAAGG AGGATGGTGT TATTAAAGAG 
TTAAGCATTG CCATGCAGCT CCTAAGAAAC TGTCTTTATC AAAATGAGGA ATGTAAAGAA 
GCAGCTCTTG AAGCTCACCT TGTCCCTGTC TTGCACTCTC TCTGGCCTTG GATTTTGATG 
GATGATTCAT TGATGCAAAT TTCTCTGCAG CTCCTTTGTG TCTATACTGC AAATTTTCCA 
AATGGTTGCA GTTCTCTTTG TTGGTCAAGT TGTGGACAAC ACCCTGTTCA AGCTACACAT 
AGAGGAGCCG TGAGCAACTC TCTGATGCTG TGTATCCTAA AGTTGGCTTC CCAGATGCCA 
CTGGAGAACA CCACGGTTCA GCAGATGGTT TTTATGCTTC TTTCAAACCT GGCCTTGTCG 
CATGACTGTA AAGGAGTAAT TCAGAAGAGT AACTTCTTAC AGAACTTCCT CTCTCTAGCA 
TTGCCAAAAG GAGGAAATAA ACATCTAAGT AATCTGACTA TTCTTTGGTT GAAGTTACTC 
CTGAATATAT CATCTGGAGA AGATGGGCAA CAAATGATTC TGAGGCTTGA TGGCTGTCTA 
GACTTACTAA CAGAGATGAG CAAATACAAG CACAAGAGCA GCCCTTTATT GCCTCTTCTT 
ATCTTTCATA ATGTTTGCTT CAGTCCTGCA AATAAACCCA AGATCCTGGC TAATGAAAAA 
GTCATTACTG TGCTTGCTGC CTGTCTGGAA AGTGAGAATC AAAATGCTCA GAGGATTGGA 
GCAGCTGCCC TTTGGGCTCT GATTTACAAT TATCAGAAGG CAAAAACAGC TTTGAAAAGC 
CCATCAGTAA AAAGAAGAGT GGATGAAGCA TACTCCTTAG CAAAGAAAAC TTTCCCAAAC 
TCAGAAGCAA ACCCTCTAAA TGCCTATTAT TTGAAATGTC TTGAAAACCT CGTGCAGCTC 
CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881185.1
CDS	2175..6749
Translation	MMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_017025696.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025696.1

ORF Insert Sequence:

ATGATGACAG CATTGACCTG GAACAAGTTT ATTGAATCTC TCTGTCCTGT CATCCCAATA 
CTACAGGGCT ATGCCGACAC AGAAGATCCT CTGGGTAACT GCATTCTCCT TCTAAGCAAA 
GCAAGTTCTG ACACAGAAGA GATGCTTCCG TGTACTACCC GATTAAAGTC CATGCTGCGA 
CTTTTGCTAG TGAAAAAGCC ATCGGTCCGT TCGCTAGCAT TAAAGCTTTT AGCATTCCAT 
CTTACCAGTG AAGAAGGGGC TGATACAAAG CGTCCTCTAA TAGACGCCAG AGTTCTCTCC 
AGAGTTACTG ATTTATTCAT TGGGAAGAAA CCTATTGAAC TCAGACTGGA TGACAGAAGA 
GAGCTGGTTA TTAAGTTGGA GACTGTTGAA AAGGTATATG AAATCTTCAC CTCAGATGAT 
GTTGATCTCG TTTTGAGAAA GTCAGCTGCT GAACAGTTAG CTGTGATTAT GCAAGATATT 
AAAATGCATG CTGTGGTGAA AAAGTTATGC TTAATTGACA AAATAATTGA GTATTTAAAT 
GAATGTGTGA GTCAAGATGG CAAGGTTGTA GAATGTTTGG TACAACCATG CCTCACACTC 
TTGAGGAAGG TTTTATGTGG TGATCCAGTC ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT 
CTTTTGACCG TGTTATTCAG AGTTTCCTTA ATATTTCATG AAGATTGTAG TGTCGTGACT 
GAAGTTGGAG CACTATTTTG TCTTCTATTA TTTGATGAAG TATCGAGAAT GGATATGTGG 
TCTGTTAATC CTTCCAATAA ACCTTCTTTG CCATCGGTCT TCAGTTTGCC TGTTTCCGTT 
TTTAGAAGGT ACCATCTACC TGTTCATGTA ATTGGACACC ATGCTGTGAG TCCTTACTCC 
ATAGTTTTGC CCTTATCTGC TGATTGTTTG GCCTTGAAGC CGGTGTCAGA TATGCTGAGA 
ATAGCTTGGA ACCTGTCATG GTATCATGGG AGTGATAATC TGTTGAAGCA AATGAACTCT 
GAAACTAAAA CGCAAGAAAT TTTAGATGCA TTGAAGTTAT CTACAGAGGA CATCCTCACT 
CTGAAGATAA CACACATGGC TAGTGGGCTG CAGGACTGCC TCCATTCCAT TGTTCAGGCT 
GCAACCCACA GGGAAGTTAG GGCTGCTGTC ACCAGGATGA GTTTTTATCT TCTGAATGAC 
AGATTGTCTT TAAAGGGTTG CCCTGGTCCA TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG 
CACACCGCAC TAAACAGGTT TTTACAGGTG CTTCCTGCTT GCACTGAAGA TGAGAAACTG 
CTAATAGATA TCATACATTT TTTAAATAAA TTAATAAAGG AACAAAGAAA AAATTCTTCA 
CTAGAACTTC TAAACTGGAT TCTCGAATTA CTTCTTAGAC ATAGTGCAAA CCCACTGTTA 
GACCTCTTGG TTCTGACAGA GTCACAGGCA CGAGAAGAAA CAGATGATAT CCGGACTGCT 
GTCAGGCAAC AACTTCAGAA AGAACTGATT GCTCTTTTTG ATACCTTGCT GCTCAATTTC 
ATGGAAGTTA CTGACAGGAA ATGCTCGGAA CTTCTTTACG TTTTTCAAAC GCAGCTGGCT 
CTGAAACTGC TCCAGTGTCT GAAAGTGACG GATGCGCCTC ATTTCTATGG CCTGCCGTCC 
CTTGAGCGGA CCTTACGAGG GATGGCTAAC CTCACTGCGT TTCCGGGATG GAGCTCACAC 
TCTCCTCTCA CAAAGCCTCT AGATATCTGT GTGAAGTACT TGTCAGGTCT CCTTGAGGTC 
ATTACTTCTT TTTATGTGGA GCGTGGAGGA AATGCTATGT CCTTCATGGG AAAAGGTGTT 
ACAAAGAGCA CAATTCTTTG CTTGCTTCAC TTATCCCATG AGATGATGGC CCAGGCTGGG 
AGCTTGGAGT GGATGTCACT TTGGTTCTTG CCTTTGGGTA GTCATAGTGA AGAACATATT 
CCTACTCAAC AAGGATTGGC TTGGTTGATT CCATTATGGG TTGATCGGGA CCCAGAGGTG 
AGATTCACTT CACTGGGATT AGGATCAGCA CTGACCACCC TTGAAACGGG CTGTGTGGCC 
TTAGCAAACA GTTGTCAGAA CATTTCCGGT GGGCTCTGGG GAACTGTGGT GAACATTCTT 
CTGGACCAGT CAGAATGTAG TATGGTGCGC CGGGAGGCGG CATTTATTCT TCAGAATCTC 
CTTGTAATTC CAATGCCTAC AGAAATTATA AAGGATTATA CTTGGCAGGG TCCCTGTGTT 
CATGATGAGG ACTCTGGCCT ATCGCTCATT GGAAAACCTG CCCTTCAGGC TCTTTTATAT 
CACTGCCATT TTTATGAACA TTTGAATCAG ATGGTAAAGC ATTGTTACCT AGGACGGTGT 
ATGTTTGATT TGAATTTTTC TGCTTTTGAT AGAAATTCAG AAAGCAATGA TTTAAATGGT 
TTAGATGACT CATTCAAGTT TTGGAGGGCT CCATCTAGGA CAAGTCAGGA TCGAGATCCA 
AGTTCTCTCT CCACCTCAGA AACAACGGTG GCACCTTCAT TGGGGAGTAC TGAATTTCAG 
CCACTTGTGC AGTCAACAAC ACTTCTACCT GAAGCCTCCC ATGACCAGTT TGTGGCTCAA 
GGTCACCAGG AAAGTACATC ACCACGGCCA CCTCATGATT CATCTCTTTC TGCTCCCCTG 
CCCAAACTGT GTGTTTTTGT TACTCCATCT CTTCTTTCAG CAATGTGCAG CCTCTTGGAC 
AACCTCTTGA CGATTGCTCC CAGAGACACT GCAAAGGCTT TTCGACAAGC TCATCTCATA 
GAACTTCTCT GTAGCATTGC AGATGCTACC CTCATACAGA CATGTGTCCA GGAACTCAGA 
GCCCTGCTGC CTTCATCACC TCCAGCTGAA CACACTCAGG CTCAGGTTTC CTTTCTCCTG 
GAATACCTAT CCTCTTTGTC CAGGCTTCTG CAGTCATGTT TATTGGTGGA GCCTGACCTT 
GTGATTCAGG ATGAGCTTGT GAAACCTCTT ATCACCAATA TCATTGGAAT TCTCACCATA 
TGTACCAAAG ATGTATTAGA TAAAGAGTTA ATATCAGCTT TTTATCACAC ATGGACACAT 
TTATTTAATC TTCTGGCCAT GCTCCTGAGG AAAGCTGGTG CCATCACACT CCCGTTTGTT 
ACCGTGGCCC TGGCCAAGCA CTGGACAGCG GCGATTGATA TGTTCTGCAC ATGTGCAGGC 
TTGTCTGCCA CGTGTCCTGC CCTGTATACT GCCAGCTTGC AATTCCTTTC TGTTCTCTTG 
ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT AAGAGCAAAA CACATTTATG CTGTAGTCCA 
ACAGTGGCTT CACTTCTTGA TGACTCTCAG GAAAATCAGA AATCTCTAGA ACAACTTAGT 
GATGTAATCC TTCAGTGCTA TGAAGGGAAA TCCTCCAAAG ATATCCTGAA AAGAGTAGCT 
GCAAATGCAT TGATGTCACT GCTGGCTGTC AGTAGAAGAG CACAGAAACA TGCTTTGAAA 
GCCAATCTTA TAGACAATTG CATGGAGCAG ATGAAACACA TAAATGCACA ACTGAACCTA 
GATTCTCTGA GGCCTGGGAA AGCAGCATTG AAAAAAAAGG AGGATGGTGT TATTAAAGAG 
TTAAGCATTG CCATGCAGCT CCTAAGAAAC TGTCTTTATC AAAATGAGGA ATGTAAAGAA 
GCAGCTCTTG AAGCTCACCT TGTCCCTGTC TTGCACTCTC TCTGGCCTTG GATTTTGATG 
GATGATTCAT TGATGCAAAT TTCTCTGCAG CTCCTTTGTG TCTATACTGC AAATTTTCCA 
AATGGTTGCA GTTCTCTTTG TTGGTCAAGT TGTGGACAAC ACCCTGTTCA AGCTACACAT 
AGAGGAGCCG TGAGCAACTC TCTGATGCTG TGTATCCTAA AGTTGGCTTC CCAGATGCCA 
CTGGAGAACA CCACGGTTCA GCAGATGGTT TTTATGCTTC TTTCAAACCT GGCCTTGTCG 
CATGACTGTA AAGGAGTAAT TCAGAAGAGT AACTTCTTAC AGAACTTCCT CTCTCTAGCA 
TTGCCAAAAG GAGGAAATAA ACATCTAAGT AATCTGACTA TTCTTTGGTT GAAGTTACTC 
CTGAATATAT CATCTGGAGA AGATGGGCAA CAAATGATTC TGAGGCTTGA TGGCTGTCTA 
GACTTACTAA CAGAGATGAG CAAATACAAG CACAAGAGCA GCCCTTTATT GCCTCTTCTT 
ATCTTTCATA ATGTTTGCTT CAGTCCTGCA AATAAACCCA AGATCCTGGC TAATGAAAAA 
GTCATTACTG TGCTTGCTGC CTGTCTGGAA AGTGAGAATC AAAATGCTCA GAGGATTGGA 
GCAGCTGCCC TTTGGGCTCT GATTTACAAT TATCAGAAGG CAAAAACAGC TTTGAAAAGC 
CCATCAGTAA AAAGAAGAGT GGATGAAGCA TACTCCTTAG CAAAGAAAAC TTTCCCAAAC 
TCAGAAGCAA ACCCTCTAAA TGCCTATTAT TTGAAATGTC TTGAAAACCT CGTGCAGCTC 
CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( NM_001318520.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305449.1 Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu51155
Clone ID Related Accession (Same CDS sequence)	NM_001318520.1 , NM_001318520.2
Accession Version	NM_001318520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	rotatin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096404.1, AC021701.8, BC143723.1, BC046222.2 and AL117635.1. On Jan 8, 2016 this sequence version replaced XM_005266679.1. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in its 5' UTR, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC143723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT CTTTTGACCG TGTTATTCAG AGTTTCCTTA 
ATATTTCATG AAGATTGTAG TGTCGTGACT GAAGTTGGAG CACTATTTTG TCTTCTATTA 
TTTGATGAAG TATCGAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTTG 
CCATCGGTCT TCAGTTTGCC TGTTTCCGTT TTTAGAAGGT ACCATCTACC TGTTCATGTA 
ATTGGACACC ATGCTGTGAG TCCTTACTCC ATAGTTTTGC CCTTATCTGC TGATTGTTTG 
GCCTTGAAGC CGGTGTCAGA TATGCTGAGA ATAGCTTGGA ACCTGTCATG GTATCATGGG 
AGTGATAATC TGTTGAAGCA AATGAACTCT GAAACTAAAA CGCAAGAAAT TTTAGATGCA 
TTGAAGTTAT CTACAGAGGA CATCCTCACT CTGAAGATAA CACACATGGC TAGTGGGCTG 
CAGGACTGCC TCCATTCCAT TGTTCAGGCT GCAACCCACA GGGAAGTTAG GGCTGCTGTC 
ACCAGGATGA GTTTTTATCT TCTGAATGAC AGATTGTCTT TAAAGGGTTG CCCTGGTCCA 
TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG CACACCGCAC TAAACAGGTT TTTACAGGTG 
CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAA 
TTAATAAAGG AACAAAGAAA AAATTCTTCA CTAGAACTTC TAAACTGGAT TCTCGAATTA 
CTTCTTAGAC ATAGTGCAAA CCCACTGTTA GACCTCTTGG TTCTGACAGA GTCACAGGCA 
CGAGAAGAAA CAGATGATAT CCGGACTGCT GTCAGGCAAC AACTTCAGAA AGAACTGATT 
GCTCTTTTTG ATACCTTGCT GCTCAATTTC ATGGAAGTTA CTGACAGGAA ATGCTCGGAA 
CTTCTTTACG TTTTTCAAAC GCAGCTGGCT CTGAAACTGC TCCAGTGTCT GAAAGTGACG 
GATGCGCCTC ATTTCTATGG CCTGCCGTCC CTTGAGCGGA CCTTACGAGG GATGGCTAAC 
CTCACTGCGT TTCCGGGATG GAGCTCACAC TCTCCTCTCA CAAAGCCTCT AGATATCTGT 
GTGAAGTACT TGTCAGGTCT CCTTGAGGTC ATTACTTCTT TTTATGTGGA GCGTGGAGGA 
AATGCTATGT CCTTCATGGG AAAAGGTGTT ACAAAGAGCA CAATTCTTTG CTTGCTTCAC 
TTATCCCATG AGATGATGGC CCAGGCTGGG AGCTTGGAGT GGATGTCACT TTGGTTCTTG 
CCTTTGGGTA GTCATAGTGA AGAACATATT CCTACTCAAC AAGGATTGGC TTGGTTGATT 
CCATTATGGG TTGATCGGGA CCCAGAGGTG AGATTCACTT CACTGGGATT AGGATCAGCA 
CTGACCACCC TTGAAACGGG CTGTGTGGCC TTAGCAAACA GTTGTCAGAA CATTTCCGGT 
GGGCTCTGGG GAACTGTGGT GAACATTCTT CTGGACCAGT CAGAATGTAG TATGGTGCGC 
CGGGAGGCGG CATTTATTCT TCAGAATCTC CTTGTAATTC CAATGCCTAC AGAAATTATA 
AAGGATTATA CTTGGCAGGG TCCCTGTGTT CATGATGAGG ACTCTGGCCT ATCGCTCATT 
GGAAAACCTG CCCTTCAGGC TCTTTTATAT CACTGCCATT TTTATGAACA TTTGAATCAG 
ATGGTAAAGC ATTGTTACCT AGGACGGTGT ATGTTTGATT TGAATTTTTC TGCTTTTGAT 
AGAAATTCAG AAAGCAATGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGGGCT 
CCATCTAGGA CAAGTCAGGA TCGAGATCCA AGTTCTCTCT CCACCTCAGA AACAACGGTG 
GCACCTTCAT TGGGGAGTAC TGAATTTCAG CCACTTGTGC AGTCAACAAC ACTTCTACCT 
GAAGCCTCCC ATGACCAGTT TGTGGCTCAA GGTCACCAGG AAAGTACATC ACCACGGCCA 
CCTCATGATT CATCTCTTTC TGCTCCCCTG CCCAAACTGT GTGTTTTTGT TACTCCATCT 
CTTCTTTCAG CAATGTGCAG CCTCTTGGAC AACCTCTTGA CGATTGCTCC CAGAGACACT 
GCAAAGGCTT TTCGACAAGC TCATCTCATA GAACTTCTCT GTAGCATTGC AGATGCTACC 
CTCATACAGA CATGTGTCCA GGAACTCAGA GCCCTGCTGC CTTCATCACC TCCAGCTGAA 
CACACTCAGG CTCAGGTTTC CTTTCTCCTG GAATACCTAT CCTCTTTGTC CAGGCTTCTG 
CAGTCATGTT TATTGGTGGA GCCTGACCTT GTGATTCAGG ATGAGCTTGT GAAACCTCTT 
ATCACCAATA TCATTGGAAT TCTCACCATA TGTACCAAAG ATGTATTAGA TAAAGAGTTA 
ATATCAGCTT TTTATCACAC ATGGACACAT TTATTTAATC TTCTGGCCAT GCTCCTGAGG 
AAAGCTGGTG CCATCACACT CCCGTTTGTT ACCGTGGCCC TGGCCAAGCA CTGGACAGCG 
GCGATTGATA TGTTCTGCAC ATGTGCAGGC TTGTCTGCCA CGTGTCCTGC CCTGTATACT 
GCCAGCTTGC AATTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT 
AAGAGCAAAA CACATTTATG CTGTAGTCCA ACAGTGGCTT CACTTCTTGA TGACTCTCAG 
GAAAATCAGA AATCTCTAGA ACAACTTAGT GATGTAATCC TTCAGTGCTA TGAAGGGAAA 
TCCTCCAAAG ATATCCTGAA AAGAGTAGCT GCAAATGCAT TGATGTCACT GCTGGCTGTC 
AGTAGAAGAG CACAGAAACA TGCTTTGAAA GCCAATCTTA TAGACAATTG CATGGAGCAG 
ATGAAACACA TAAATGCACA ACTGAACCTA GATTCTCTGA GGCCTGGGAA AGCAGCATTG 
AAAAAAAAGG AGGATGGTGT TATTAAAGAG TTAAGCATTG CCATGCAGCT CCTAAGAAAC 
TGTCTTTATC AAAATGAGGA ATGTAAAGAA GCAGCTCTTG AAGCTCACCT TGTCCCTGTC 
TTGCACTCTC TCTGGCCTTG GATTTTGATG GATGATTCAT TGATGCAAAT TTCTCTGCAG 
CTCCTTTGTG TCTATACTGC AAATTTTCCA AATGGTTGCA GTTCTCTTTG TTGGTCAAGT 
TGTGGACAAC ACCCTGTTCA AGCTACACAT AGAGGAGCCG TGAGCAACTC TCTGATGCTG 
TGTATCCTAA AGTTGGCTTC CCAGATGCCA CTGGAGAACA CCACGGTTCA GCAGATGGTT 
TTTATGCTTC TTTCAAACCT GGCCTTGTCG CATGACTGTA AAGGAGTAAT TCAGAAGAGT 
AACTTCTTAC AGAACTTCCT CTCTCTAGCA TTGCCAAAAG GAGGAAATAA ACATCTAAGT 
AATCTGACTA TTCTTTGGTT GAAGTTACTC CTGAATATAT CATCTGGAGA AGATGGGCAA 
CAAATGATTC TGAGGCTTGA TGGCTGTCTA GACTTACTAA CAGAGATGAG CAAATACAAG 
CACAAGAGCA GCCCTTTATT GCCTCTTCTT ATCTTTCATA ATGTTTGCTT CAGTCCTGCA 
AATAAACCCA AGATCCTGGC TAATGAAAAA GTCATTACTG TGCTTGCTGC CTGTCTGGAA 
AGTGAGAATC AAAATGCTCA GAGGATTGGA GCAGCTGCCC TTTGGGCTCT GATTTACAAT 
TATCAGAAGG CAAAAACAGC TTTGAAAAGC CCATCAGTAA AAAGAAGAGT GGATGAAGCA 
TACTCCTTAG CAAAGAAAAC TTTCCCAAAC TCAGAAGCAA ACCCTCTAAA TGCCTATTAT 
TTGAAATGTC TTGAAAACCT CGTGCAGCTC CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305449.1
CDS	2622..6566
Translation	MRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	NM_001318520.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318520.1

ORF Insert Sequence:

ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT CTTTTGACCG TGTTATTCAG AGTTTCCTTA 
ATATTTCATG AAGATTGTAG TGTCGTGACT GAAGTTGGAG CACTATTTTG TCTTCTATTA 
TTTGATGAAG TATCGAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTTG 
CCATCGGTCT TCAGTTTGCC TGTTTCCGTT TTTAGAAGGT ACCATCTACC TGTTCATGTA 
ATTGGACACC ATGCTGTGAG TCCTTACTCC ATAGTTTTGC CCTTATCTGC TGATTGTTTG 
GCCTTGAAGC CGGTGTCAGA TATGCTGAGA ATAGCTTGGA ACCTGTCATG GTATCATGGG 
AGTGATAATC TGTTGAAGCA AATGAACTCT GAAACTAAAA CGCAAGAAAT TTTAGATGCA 
TTGAAGTTAT CTACAGAGGA CATCCTCACT CTGAAGATAA CACACATGGC TAGTGGGCTG 
CAGGACTGCC TCCATTCCAT TGTTCAGGCT GCAACCCACA GGGAAGTTAG GGCTGCTGTC 
ACCAGGATGA GTTTTTATCT TCTGAATGAC AGATTGTCTT TAAAGGGTTG CCCTGGTCCA 
TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG CACACCGCAC TAAACAGGTT TTTACAGGTG 
CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAA 
TTAATAAAGG AACAAAGAAA AAATTCTTCA CTAGAACTTC TAAACTGGAT TCTCGAATTA 
CTTCTTAGAC ATAGTGCAAA CCCACTGTTA GACCTCTTGG TTCTGACAGA GTCACAGGCA 
CGAGAAGAAA CAGATGATAT CCGGACTGCT GTCAGGCAAC AACTTCAGAA AGAACTGATT 
GCTCTTTTTG ATACCTTGCT GCTCAATTTC ATGGAAGTTA CTGACAGGAA ATGCTCGGAA 
CTTCTTTACG TTTTTCAAAC GCAGCTGGCT CTGAAACTGC TCCAGTGTCT GAAAGTGACG 
GATGCGCCTC ATTTCTATGG CCTGCCGTCC CTTGAGCGGA CCTTACGAGG GATGGCTAAC 
CTCACTGCGT TTCCGGGATG GAGCTCACAC TCTCCTCTCA CAAAGCCTCT AGATATCTGT 
GTGAAGTACT TGTCAGGTCT CCTTGAGGTC ATTACTTCTT TTTATGTGGA GCGTGGAGGA 
AATGCTATGT CCTTCATGGG AAAAGGTGTT ACAAAGAGCA CAATTCTTTG CTTGCTTCAC 
TTATCCCATG AGATGATGGC CCAGGCTGGG AGCTTGGAGT GGATGTCACT TTGGTTCTTG 
CCTTTGGGTA GTCATAGTGA AGAACATATT CCTACTCAAC AAGGATTGGC TTGGTTGATT 
CCATTATGGG TTGATCGGGA CCCAGAGGTG AGATTCACTT CACTGGGATT AGGATCAGCA 
CTGACCACCC TTGAAACGGG CTGTGTGGCC TTAGCAAACA GTTGTCAGAA CATTTCCGGT 
GGGCTCTGGG GAACTGTGGT GAACATTCTT CTGGACCAGT CAGAATGTAG TATGGTGCGC 
CGGGAGGCGG CATTTATTCT TCAGAATCTC CTTGTAATTC CAATGCCTAC AGAAATTATA 
AAGGATTATA CTTGGCAGGG TCCCTGTGTT CATGATGAGG ACTCTGGCCT ATCGCTCATT 
GGAAAACCTG CCCTTCAGGC TCTTTTATAT CACTGCCATT TTTATGAACA TTTGAATCAG 
ATGGTAAAGC ATTGTTACCT AGGACGGTGT ATGTTTGATT TGAATTTTTC TGCTTTTGAT 
AGAAATTCAG AAAGCAATGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGGGCT 
CCATCTAGGA CAAGTCAGGA TCGAGATCCA AGTTCTCTCT CCACCTCAGA AACAACGGTG 
GCACCTTCAT TGGGGAGTAC TGAATTTCAG CCACTTGTGC AGTCAACAAC ACTTCTACCT 
GAAGCCTCCC ATGACCAGTT TGTGGCTCAA GGTCACCAGG AAAGTACATC ACCACGGCCA 
CCTCATGATT CATCTCTTTC TGCTCCCCTG CCCAAACTGT GTGTTTTTGT TACTCCATCT 
CTTCTTTCAG CAATGTGCAG CCTCTTGGAC AACCTCTTGA CGATTGCTCC CAGAGACACT 
GCAAAGGCTT TTCGACAAGC TCATCTCATA GAACTTCTCT GTAGCATTGC AGATGCTACC 
CTCATACAGA CATGTGTCCA GGAACTCAGA GCCCTGCTGC CTTCATCACC TCCAGCTGAA 
CACACTCAGG CTCAGGTTTC CTTTCTCCTG GAATACCTAT CCTCTTTGTC CAGGCTTCTG 
CAGTCATGTT TATTGGTGGA GCCTGACCTT GTGATTCAGG ATGAGCTTGT GAAACCTCTT 
ATCACCAATA TCATTGGAAT TCTCACCATA TGTACCAAAG ATGTATTAGA TAAAGAGTTA 
ATATCAGCTT TTTATCACAC ATGGACACAT TTATTTAATC TTCTGGCCAT GCTCCTGAGG 
AAAGCTGGTG CCATCACACT CCCGTTTGTT ACCGTGGCCC TGGCCAAGCA CTGGACAGCG 
GCGATTGATA TGTTCTGCAC ATGTGCAGGC TTGTCTGCCA CGTGTCCTGC CCTGTATACT 
GCCAGCTTGC AATTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT 
AAGAGCAAAA CACATTTATG CTGTAGTCCA ACAGTGGCTT CACTTCTTGA TGACTCTCAG 
GAAAATCAGA AATCTCTAGA ACAACTTAGT GATGTAATCC TTCAGTGCTA TGAAGGGAAA 
TCCTCCAAAG ATATCCTGAA AAGAGTAGCT GCAAATGCAT TGATGTCACT GCTGGCTGTC 
AGTAGAAGAG CACAGAAACA TGCTTTGAAA GCCAATCTTA TAGACAATTG CATGGAGCAG 
ATGAAACACA TAAATGCACA ACTGAACCTA GATTCTCTGA GGCCTGGGAA AGCAGCATTG 
AAAAAAAAGG AGGATGGTGT TATTAAAGAG TTAAGCATTG CCATGCAGCT CCTAAGAAAC 
TGTCTTTATC AAAATGAGGA ATGTAAAGAA GCAGCTCTTG AAGCTCACCT TGTCCCTGTC 
TTGCACTCTC TCTGGCCTTG GATTTTGATG GATGATTCAT TGATGCAAAT TTCTCTGCAG 
CTCCTTTGTG TCTATACTGC AAATTTTCCA AATGGTTGCA GTTCTCTTTG TTGGTCAAGT 
TGTGGACAAC ACCCTGTTCA AGCTACACAT AGAGGAGCCG TGAGCAACTC TCTGATGCTG 
TGTATCCTAA AGTTGGCTTC CCAGATGCCA CTGGAGAACA CCACGGTTCA GCAGATGGTT 
TTTATGCTTC TTTCAAACCT GGCCTTGTCG CATGACTGTA AAGGAGTAAT TCAGAAGAGT 
AACTTCTTAC AGAACTTCCT CTCTCTAGCA TTGCCAAAAG GAGGAAATAA ACATCTAAGT 
AATCTGACTA TTCTTTGGTT GAAGTTACTC CTGAATATAT CATCTGGAGA AGATGGGCAA 
CAAATGATTC TGAGGCTTGA TGGCTGTCTA GACTTACTAA CAGAGATGAG CAAATACAAG 
CACAAGAGCA GCCCTTTATT GCCTCTTCTT ATCTTTCATA ATGTTTGCTT CAGTCCTGCA 
AATAAACCCA AGATCCTGGC TAATGAAAAA GTCATTACTG TGCTTGCTGC CTGTCTGGAA 
AGTGAGAATC AAAATGCTCA GAGGATTGGA GCAGCTGCCC TTTGGGCTCT GATTTACAAT 
TATCAGAAGG CAAAAACAGC TTTGAAAAGC CCATCAGTAA AAAGAAGAGT GGATGAAGCA 
TACTCCTTAG CAAAGAAAAC TTTCCCAAAC TCAGAAGCAA ACCCTCTAAA TGCCTATTAT 
TTGAAATGTC TTGAAAACCT CGTGCAGCTC CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( NM_001318520.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305449.1 Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu51155
Clone ID Related Accession (Same CDS sequence)	NM_001318520.1 , NM_001318520.2
Accession Version	NM_001318520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	rotatin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096404.1, AC021701.8, BC143723.1, BC046222.2, AL117635.1 and AC011930.5. On May 31, 2019 this sequence version replaced NM_001318520.1. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in its 5' UTR, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC143723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT CTTTTGACCG TGTTATTCAG AGTTTCCTTA 
ATATTTCATG AAGATTGTAG TGTCGTGACT GAAGTTGGAG CACTATTTTG TCTTCTATTA 
TTTGATGAAG TATCGAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTTG 
CCATCGGTCT TCAGTTTGCC TGTTTCCGTT TTTAGAAGGT ACCATCTACC TGTTCATGTA 
ATTGGACACC ATGCTGTGAG TCCTTACTCC ATAGTTTTGC CCTTATCTGC TGATTGTTTG 
GCCTTGAAGC CGGTGTCAGA TATGCTGAGA ATAGCTTGGA ACCTGTCATG GTATCATGGG 
AGTGATAATC TGTTGAAGCA AATGAACTCT GAAACTAAAA CGCAAGAAAT TTTAGATGCA 
TTGAAGTTAT CTACAGAGGA CATCCTCACT CTGAAGATAA CACACATGGC TAGTGGGCTG 
CAGGACTGCC TCCATTCCAT TGTTCAGGCT GCAACCCACA GGGAAGTTAG GGCTGCTGTC 
ACCAGGATGA GTTTTTATCT TCTGAATGAC AGATTGTCTT TAAAGGGTTG CCCTGGTCCA 
TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG CACACCGCAC TAAACAGGTT TTTACAGGTG 
CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAA 
TTAATAAAGG AACAAAGAAA AAATTCTTCA CTAGAACTTC TAAACTGGAT TCTCGAATTA 
CTTCTTAGAC ATAGTGCAAA CCCACTGTTA GACCTCTTGG TTCTGACAGA GTCACAGGCA 
CGAGAAGAAA CAGATGATAT CCGGACTGCT GTCAGGCAAC AACTTCAGAA AGAACTGATT 
GCTCTTTTTG ATACCTTGCT GCTCAATTTC ATGGAAGTTA CTGACAGGAA ATGCTCGGAA 
CTTCTTTACG TTTTTCAAAC GCAGCTGGCT CTGAAACTGC TCCAGTGTCT GAAAGTGACG 
GATGCGCCTC ATTTCTATGG CCTGCCGTCC CTTGAGCGGA CCTTACGAGG GATGGCTAAC 
CTCACTGCGT TTCCGGGATG GAGCTCACAC TCTCCTCTCA CAAAGCCTCT AGATATCTGT 
GTGAAGTACT TGTCAGGTCT CCTTGAGGTC ATTACTTCTT TTTATGTGGA GCGTGGAGGA 
AATGCTATGT CCTTCATGGG AAAAGGTGTT ACAAAGAGCA CAATTCTTTG CTTGCTTCAC 
TTATCCCATG AGATGATGGC CCAGGCTGGG AGCTTGGAGT GGATGTCACT TTGGTTCTTG 
CCTTTGGGTA GTCATAGTGA AGAACATATT CCTACTCAAC AAGGATTGGC TTGGTTGATT 
CCATTATGGG TTGATCGGGA CCCAGAGGTG AGATTCACTT CACTGGGATT AGGATCAGCA 
CTGACCACCC TTGAAACGGG CTGTGTGGCC TTAGCAAACA GTTGTCAGAA CATTTCCGGT 
GGGCTCTGGG GAACTGTGGT GAACATTCTT CTGGACCAGT CAGAATGTAG TATGGTGCGC 
CGGGAGGCGG CATTTATTCT TCAGAATCTC CTTGTAATTC CAATGCCTAC AGAAATTATA 
AAGGATTATA CTTGGCAGGG TCCCTGTGTT CATGATGAGG ACTCTGGCCT ATCGCTCATT 
GGAAAACCTG CCCTTCAGGC TCTTTTATAT CACTGCCATT TTTATGAACA TTTGAATCAG 
ATGGTAAAGC ATTGTTACCT AGGACGGTGT ATGTTTGATT TGAATTTTTC TGCTTTTGAT 
AGAAATTCAG AAAGCAATGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGGGCT 
CCATCTAGGA CAAGTCAGGA TCGAGATCCA AGTTCTCTCT CCACCTCAGA AACAACGGTG 
GCACCTTCAT TGGGGAGTAC TGAATTTCAG CCACTTGTGC AGTCAACAAC ACTTCTACCT 
GAAGCCTCCC ATGACCAGTT TGTGGCTCAA GGTCACCAGG AAAGTACATC ACCACGGCCA 
CCTCATGATT CATCTCTTTC TGCTCCCCTG CCCAAACTGT GTGTTTTTGT TACTCCATCT 
CTTCTTTCAG CAATGTGCAG CCTCTTGGAC AACCTCTTGA CGATTGCTCC CAGAGACACT 
GCAAAGGCTT TTCGACAAGC TCATCTCATA GAACTTCTCT GTAGCATTGC AGATGCTACC 
CTCATACAGA CATGTGTCCA GGAACTCAGA GCCCTGCTGC CTTCATCACC TCCAGCTGAA 
CACACTCAGG CTCAGGTTTC CTTTCTCCTG GAATACCTAT CCTCTTTGTC CAGGCTTCTG 
CAGTCATGTT TATTGGTGGA GCCTGACCTT GTGATTCAGG ATGAGCTTGT GAAACCTCTT 
ATCACCAATA TCATTGGAAT TCTCACCATA TGTACCAAAG ATGTATTAGA TAAAGAGTTA 
ATATCAGCTT TTTATCACAC ATGGACACAT TTATTTAATC TTCTGGCCAT GCTCCTGAGG 
AAAGCTGGTG CCATCACACT CCCGTTTGTT ACCGTGGCCC TGGCCAAGCA CTGGACAGCG 
GCGATTGATA TGTTCTGCAC ATGTGCAGGC TTGTCTGCCA CGTGTCCTGC CCTGTATACT 
GCCAGCTTGC AATTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT 
AAGAGCAAAA CACATTTATG CTGTAGTCCA ACAGTGGCTT CACTTCTTGA TGACTCTCAG 
GAAAATCAGA AATCTCTAGA ACAACTTAGT GATGTAATCC TTCAGTGCTA TGAAGGGAAA 
TCCTCCAAAG ATATCCTGAA AAGAGTAGCT GCAAATGCAT TGATGTCACT GCTGGCTGTC 
AGTAGAAGAG CACAGAAACA TGCTTTGAAA GCCAATCTTA TAGACAATTG CATGGAGCAG 
ATGAAACACA TAAATGCACA ACTGAACCTA GATTCTCTGA GGCCTGGGAA AGCAGCATTG 
AAAAAAAAGG AGGATGGTGT TATTAAAGAG TTAAGCATTG CCATGCAGCT CCTAAGAAAC 
TGTCTTTATC AAAATGAGGA ATGTAAAGAA GCAGCTCTTG AAGCTCACCT TGTCCCTGTC 
TTGCACTCTC TCTGGCCTTG GATTTTGATG GATGATTCAT TGATGCAAAT TTCTCTGCAG 
CTCCTTTGTG TCTATACTGC AAATTTTCCA AATGGTTGCA GTTCTCTTTG TTGGTCAAGT 
TGTGGACAAC ACCCTGTTCA AGCTACACAT AGAGGAGCCG TGAGCAACTC TCTGATGCTG 
TGTATCCTAA AGTTGGCTTC CCAGATGCCA CTGGAGAACA CCACGGTTCA GCAGATGGTT 
TTTATGCTTC TTTCAAACCT GGCCTTGTCG CATGACTGTA AAGGAGTAAT TCAGAAGAGT 
AACTTCTTAC AGAACTTCCT CTCTCTAGCA TTGCCAAAAG GAGGAAATAA ACATCTAAGT 
AATCTGACTA TTCTTTGGTT GAAGTTACTC CTGAATATAT CATCTGGAGA AGATGGGCAA 
CAAATGATTC TGAGGCTTGA TGGCTGTCTA GACTTACTAA CAGAGATGAG CAAATACAAG 
CACAAGAGCA GCCCTTTATT GCCTCTTCTT ATCTTTCATA ATGTTTGCTT CAGTCCTGCA 
AATAAACCCA AGATCCTGGC TAATGAAAAA GTCATTACTG TGCTTGCTGC CTGTCTGGAA 
AGTGAGAATC AAAATGCTCA GAGGATTGGA GCAGCTGCCC TTTGGGCTCT GATTTACAAT 
TATCAGAAGG CAAAAACAGC TTTGAAAAGC CCATCAGTAA AAAGAAGAGT GGATGAAGCA 
TACTCCTTAG CAAAGAAAAC TTTCCCAAAC TCAGAAGCAA ACCCTCTAAA TGCCTATTAT 
TTGAAATGTC TTGAAAACCT CGTGCAGCTC CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305449.1
CDS	2583..6527
Translation	MRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	NM_001318520.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318520.2

ORF Insert Sequence:

ATGCGTGTTT CGCTCTCGCA ACAGTCTTCT CTTTTGACCG TGTTATTCAG AGTTTCCTTA 
ATATTTCATG AAGATTGTAG TGTCGTGACT GAAGTTGGAG CACTATTTTG TCTTCTATTA 
TTTGATGAAG TATCGAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTTG 
CCATCGGTCT TCAGTTTGCC TGTTTCCGTT TTTAGAAGGT ACCATCTACC TGTTCATGTA 
ATTGGACACC ATGCTGTGAG TCCTTACTCC ATAGTTTTGC CCTTATCTGC TGATTGTTTG 
GCCTTGAAGC CGGTGTCAGA TATGCTGAGA ATAGCTTGGA ACCTGTCATG GTATCATGGG 
AGTGATAATC TGTTGAAGCA AATGAACTCT GAAACTAAAA CGCAAGAAAT TTTAGATGCA 
TTGAAGTTAT CTACAGAGGA CATCCTCACT CTGAAGATAA CACACATGGC TAGTGGGCTG 
CAGGACTGCC TCCATTCCAT TGTTCAGGCT GCAACCCACA GGGAAGTTAG GGCTGCTGTC 
ACCAGGATGA GTTTTTATCT TCTGAATGAC AGATTGTCTT TAAAGGGTTG CCCTGGTCCA 
TGTGGGGTTA CCTTGAAGTC CTTGGCTTGG CACACCGCAC TAAACAGGTT TTTACAGGTG 
CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAA 
TTAATAAAGG AACAAAGAAA AAATTCTTCA CTAGAACTTC TAAACTGGAT TCTCGAATTA 
CTTCTTAGAC ATAGTGCAAA CCCACTGTTA GACCTCTTGG TTCTGACAGA GTCACAGGCA 
CGAGAAGAAA CAGATGATAT CCGGACTGCT GTCAGGCAAC AACTTCAGAA AGAACTGATT 
GCTCTTTTTG ATACCTTGCT GCTCAATTTC ATGGAAGTTA CTGACAGGAA ATGCTCGGAA 
CTTCTTTACG TTTTTCAAAC GCAGCTGGCT CTGAAACTGC TCCAGTGTCT GAAAGTGACG 
GATGCGCCTC ATTTCTATGG CCTGCCGTCC CTTGAGCGGA CCTTACGAGG GATGGCTAAC 
CTCACTGCGT TTCCGGGATG GAGCTCACAC TCTCCTCTCA CAAAGCCTCT AGATATCTGT 
GTGAAGTACT TGTCAGGTCT CCTTGAGGTC ATTACTTCTT TTTATGTGGA GCGTGGAGGA 
AATGCTATGT CCTTCATGGG AAAAGGTGTT ACAAAGAGCA CAATTCTTTG CTTGCTTCAC 
TTATCCCATG AGATGATGGC CCAGGCTGGG AGCTTGGAGT GGATGTCACT TTGGTTCTTG 
CCTTTGGGTA GTCATAGTGA AGAACATATT CCTACTCAAC AAGGATTGGC TTGGTTGATT 
CCATTATGGG TTGATCGGGA CCCAGAGGTG AGATTCACTT CACTGGGATT AGGATCAGCA 
CTGACCACCC TTGAAACGGG CTGTGTGGCC TTAGCAAACA GTTGTCAGAA CATTTCCGGT 
GGGCTCTGGG GAACTGTGGT GAACATTCTT CTGGACCAGT CAGAATGTAG TATGGTGCGC 
CGGGAGGCGG CATTTATTCT TCAGAATCTC CTTGTAATTC CAATGCCTAC AGAAATTATA 
AAGGATTATA CTTGGCAGGG TCCCTGTGTT CATGATGAGG ACTCTGGCCT ATCGCTCATT 
GGAAAACCTG CCCTTCAGGC TCTTTTATAT CACTGCCATT TTTATGAACA TTTGAATCAG 
ATGGTAAAGC ATTGTTACCT AGGACGGTGT ATGTTTGATT TGAATTTTTC TGCTTTTGAT 
AGAAATTCAG AAAGCAATGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGGGCT 
CCATCTAGGA CAAGTCAGGA TCGAGATCCA AGTTCTCTCT CCACCTCAGA AACAACGGTG 
GCACCTTCAT TGGGGAGTAC TGAATTTCAG CCACTTGTGC AGTCAACAAC ACTTCTACCT 
GAAGCCTCCC ATGACCAGTT TGTGGCTCAA GGTCACCAGG AAAGTACATC ACCACGGCCA 
CCTCATGATT CATCTCTTTC TGCTCCCCTG CCCAAACTGT GTGTTTTTGT TACTCCATCT 
CTTCTTTCAG CAATGTGCAG CCTCTTGGAC AACCTCTTGA CGATTGCTCC CAGAGACACT 
GCAAAGGCTT TTCGACAAGC TCATCTCATA GAACTTCTCT GTAGCATTGC AGATGCTACC 
CTCATACAGA CATGTGTCCA GGAACTCAGA GCCCTGCTGC CTTCATCACC TCCAGCTGAA 
CACACTCAGG CTCAGGTTTC CTTTCTCCTG GAATACCTAT CCTCTTTGTC CAGGCTTCTG 
CAGTCATGTT TATTGGTGGA GCCTGACCTT GTGATTCAGG ATGAGCTTGT GAAACCTCTT 
ATCACCAATA TCATTGGAAT TCTCACCATA TGTACCAAAG ATGTATTAGA TAAAGAGTTA 
ATATCAGCTT TTTATCACAC ATGGACACAT TTATTTAATC TTCTGGCCAT GCTCCTGAGG 
AAAGCTGGTG CCATCACACT CCCGTTTGTT ACCGTGGCCC TGGCCAAGCA CTGGACAGCG 
GCGATTGATA TGTTCTGCAC ATGTGCAGGC TTGTCTGCCA CGTGTCCTGC CCTGTATACT 
GCCAGCTTGC AATTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT 
AAGAGCAAAA CACATTTATG CTGTAGTCCA ACAGTGGCTT CACTTCTTGA TGACTCTCAG 
GAAAATCAGA AATCTCTAGA ACAACTTAGT GATGTAATCC TTCAGTGCTA TGAAGGGAAA 
TCCTCCAAAG ATATCCTGAA AAGAGTAGCT GCAAATGCAT TGATGTCACT GCTGGCTGTC 
AGTAGAAGAG CACAGAAACA TGCTTTGAAA GCCAATCTTA TAGACAATTG CATGGAGCAG 
ATGAAACACA TAAATGCACA ACTGAACCTA GATTCTCTGA GGCCTGGGAA AGCAGCATTG 
AAAAAAAAGG AGGATGGTGT TATTAAAGAG TTAAGCATTG CCATGCAGCT CCTAAGAAAC 
TGTCTTTATC AAAATGAGGA ATGTAAAGAA GCAGCTCTTG AAGCTCACCT TGTCCCTGTC 
TTGCACTCTC TCTGGCCTTG GATTTTGATG GATGATTCAT TGATGCAAAT TTCTCTGCAG 
CTCCTTTGTG TCTATACTGC AAATTTTCCA AATGGTTGCA GTTCTCTTTG TTGGTCAAGT 
TGTGGACAAC ACCCTGTTCA AGCTACACAT AGAGGAGCCG TGAGCAACTC TCTGATGCTG 
TGTATCCTAA AGTTGGCTTC CCAGATGCCA CTGGAGAACA CCACGGTTCA GCAGATGGTT 
TTTATGCTTC TTTCAAACCT GGCCTTGTCG CATGACTGTA AAGGAGTAAT TCAGAAGAGT 
AACTTCTTAC AGAACTTCCT CTCTCTAGCA TTGCCAAAAG GAGGAAATAA ACATCTAAGT 
AATCTGACTA TTCTTTGGTT GAAGTTACTC CTGAATATAT CATCTGGAGA AGATGGGCAA 
CAAATGATTC TGAGGCTTGA TGGCTGTCTA GACTTACTAA CAGAGATGAG CAAATACAAG 
CACAAGAGCA GCCCTTTATT GCCTCTTCTT ATCTTTCATA ATGTTTGCTT CAGTCCTGCA 
AATAAACCCA AGATCCTGGC TAATGAAAAA GTCATTACTG TGCTTGCTGC CTGTCTGGAA 
AGTGAGAATC AAAATGCTCA GAGGATTGGA GCAGCTGCCC TTTGGGCTCT GATTTACAAT 
TATCAGAAGG CAAAAACAGC TTTGAAAAGC CCATCAGTAA AAAGAAGAGT GGATGAAGCA 
TACTCCTTAG CAAAGAAAAC TTTCCCAAAC TCAGAAGCAA ACCCTCTAAA TGCCTATTAT 
TTGAAATGTC TTGAAAACCT CGTGCAGCTC CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_024451139.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306907.1 Homo sapiens rotatin (RTTN), transcript variant X8, mRNA.

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CloneID	OHu104535
Clone ID Related Accession (Same CDS sequence)	XM_024451139.1 , XM_024451140.1
Accession Version	XM_024451139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306907.1
CDS	289..6192
Translation	MYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_024451139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451139.1

ORF Insert Sequence:

ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_024451140.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306908.1 Homo sapiens rotatin (RTTN), transcript variant X9, mRNA.

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CloneID	OHu104535
Clone ID Related Accession (Same CDS sequence)	XM_024451139.1 , XM_024451140.1
Accession Version	XM_024451140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306908.1
CDS	373..6276
Translation	MYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_024451140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451140.1

ORF Insert Sequence:

ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_017025695.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881184.1 Homo sapiens rotatin (RTTN), transcript variant X11, mRNA.

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CloneID	OHu87843
Clone ID Related Accession (Same CDS sequence)	XM_017025695.1
Accession Version	XM_017025695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA TAGATCTGCC AGAGCTGGAA ACTGAAGACA CATTGGAACT ACAATTCCAG 
CAGCTCAGTC TTCCCCAGTT TTGTGTCTCC ATTCTGGAAT CAGCTGTTCC TCTCTTAAGA 
ACAGGTAGCA GACAAGTGAT AATAAGAGTT CTGGAATTGC TTACAGAGGA TATGACACTT 
ATTGGTGAAG CAATTTCAAC AGATATCTGG GATGACAGCA GCCTTTTTGG TATAGATATG 
AAGGAGAAAC TACTCCTGGT GTTGGGAGCC CTTGGAGAAA CCATGTGCTA CCATAAAAGT 
AGTATCAGTT TGGAGCAGCC AGAGGTGATG CTTGTGCACC ACAGAATGGC CTTTATCAGC 
ATTTCCCTGT TTGCAGTTCG ACTGCTACAA ACGCTTCTCC CTGTTGAAAA GGCAAGCGAG 
TTTTTATCAG AGCCTATGTC AACAGCATTA TTTCTCCTTT CTTTGGACAT GCCTATTTCT 
TTGGAATATC CAAATATTCA TGAAGCTGTT GTGGCCTATT TGGAACAGCT GAATTCTGAA 
AACTACAGTA TTTATAAACG AACTGCAGAG GCCGTTTATT CAATTGAATG CACCTGTAAC 
TTCCTGTCTG ATATTGGGAA AGAGGGAGAG AAGAATCTCT TAGAATTAGT GGAGCTGGCA 
GACCAAGCCT TGCGTAGCTT TTCCTATCAT CAGCATTTCC CGCTAATAAA GGAAATCATC 
AGCATTTGTT CAAAGATCTG GAAATCTGCT CAGGCCAGTC CATTACTACA AGGAGAAAGT 
CAGAAGGTGC TTCTCCATAT GTTGTCTCAC CCATTGCCAC GAGTGAAAGC TGAAACTTAC 
CACTGCTGTC TGGAAATCAC GAAGGAATGT TTAGGTGTCC ATAATGTCAC TAAACCCGTG 
TCTTCACTTT GCAATGGAAT TCATTTTCTA CTTCATCCAA AAGTTCTGTA TGAAATCTCT 
GTATTTGGCA TTCAAGAGCC CGAAAGTGAG CAGGTGAACA CTGCTGCCAA GGCCATTCTG 
TTATACCTGC TTCAGGGACG ATTGATGATG ACAGCATTGA CCTGGAACAA GTTTATTGAA 
TCTCTCTGTC CTGTCATCCC AATACTACAG GGCTATGCCG ACACAGAAGA TCCTCTGGGT 
AACTGCATTC TCCTTCTAAG CAAAGCAAGT TCTGACACAG AAGAGATGCT TCCGTGTACT 
ACCCGATTAA AGTCCATGCT GCGACTTTTG CTAGTGAAAA AGCCATCGGT CCGTTCGCTA 
GCATTAAAGC TTTTAGCATT CCATCTTACC AGTGAAGAAG GGGCTGATAC AAAGCGTCCT 
CTAATAGACG CCAGAGTTCT CTCCAGAGTT ACTGATTTAT TCATTGGGAA GAAACCTATT 
GAACTCAGAC TGGATGACAG AAGAGAGCTG GTTATTAAGT TGGAGACTGT TGAAAAGGTA 
TATGAAATCT TCACCTCAGA TGATGTTGAT CTCGTTTTGA GAAAGTCAGC TGCTGAACAG 
TTAGCTGTGA TTATGCAAGA TATTAAAATG CATGCTGTGG TGAAAAAGTT ATGCTTAATT 
GACAAAATAA TTGAGTATTT AAATGAATGT GTGAGTCAAG ATGGCAAGGT TGTAGAATGT 
TTGGTACAAC CATGCCTCAC ACTCTTGAGG AAGGTTTTAT GTGGTGATCC AGTCATGCGT 
GTTTCGCTCT CGCAACAGTC TTCTCTTTTG ACCGTGTTAT TCAGAGTTTC CTTAATATTT 
CATGAAGATT GTAGTGTCGT GACTGAAGTT GGAGCACTAT TTTGTCTTCT ATTATTTGAT 
GAAGTATCGA GAATGGATAT GTGGTCTGTT AATCCTTCCA ATAAACCTTC TTTGCCATCG 
GTCTTCAGTT TGCCTGTTTC CGTTTTTAGA AGGTACCATC TACCTGTTCA TGTAATTGGA 
CACCATGCTG TGAGTCCTTA CTCCATAGTT TTGCCCTTAT CTGCTGATTG TTTGGCCTTG 
AAGCCGGTGT CAGATATGCT GAGAATAGCT TGGAACCTGT CATGGTATCA TGGGAGTGAT 
AATCTGTTGA AGCAAATGAA CTCTGAAACT AAAACGCAAG AAATTTTAGA TGCATTGAAG 
TTATCTACAG AGGACATCCT CACTCTGAAG ATAACACACA TGGCTAGTGG GCTGCAGGAC 
TGCCTCCATT CCATTGTTCA GGCTGCAACC CACAGGGAAG TTAGGGCTGC TGTCACCAGG 
ATGAGTTTTT ATCTTCTGAA TGACAGATTG TCTTTAAAGG GTTGCCCTGG TCCATGTGGG 
GTTACCTTGA AGTCCTTGGC TTGGCACACC GCACTAAACA GGTTTTTACA GGTGCTTCCT 
GCTTGCACTG AAGATGAGAA ACTGCTAATA GATATCATAC ATTTTTTAAA TAAATTAATA 
AAGGAACAAA GAAAAAATTC TTCACTAGAA CTTCTAAACT GGATTCTCGA ATTACTTCTT 
AGACATAGTG CAAACCCACT GTTAGACCTC TTGGTTCTGA CAGAGTCACA GGCACGAGAA 
GAAACAGATG ATATCCGGAC TGCTGTCAGG CAACAACTTC AGAAAGAACT GATTGCTCTT 
TTTGATACCT TGCTGCTCAA TTTCATGGAA GTTACTGACA GGAAATGCTC GGAACTTCTT 
TACGTTTTTC AAACGCAGCT GGCTCTGAAA CTGCTCCAGT GTCTGAAAGT GACGGATGCG 
CCTCATTTCT ATGGCCTGCC GTCCCTTGAG CGGACCTTAC GAGGGATGGC TAACCTCACT 
GCGTTTCCGG GATGGAGCTC ACACTCTCCT CTCACAAAGC CTCTAGATAT CTGTGTGAAG 
TACTTGTCAG GTCTCCTTGA GGTCATTACT TCTTTTTATG TGGAGCGTGG AGGAAATGCT 
ATGTCCTTCA TGGGAAAAGG TGTTACAAAG AGCACAATTC TTTGCTTGCT TCACTTATCC 
CATGAGATGA TGGCCCAGGC TGGGAGCTTG GAGTGGATGT CACTTTGGTT CTTGCCTTTG 
GGTAGTCATA GTGAAGAACA TATTCCTACT CAACAAGGAT TGGCTTGGTT GATTCCATTA 
TGGGTTGATC GGGACCCAGA GGTGAGATTC ACTTCACTGG GATTAGGATC AGCACTGACC 
ACCCTTGAAA CGGGCTGTGT GGCCTTAGCA AACAGTTGTC AGAACATTTC CGGTGGGCTC 
TGGGGAACTG TGGTGAACAT TCTTCTGGAC CAGTCAGAAT GTAGTATGGT GCGCCGGGAG 
GCGGCATTTA TTCTTCAGAA TCTCCTTGTA ATTCCAATGC CTACAGAAAT TATAAAGGAT 
TATACTTGGC AGGGTCCCTG TGTTCATGAT GAGGACTCTG GCCTATCGCT CATTGGAAAA 
CCTGCCCTTC AGGCTCTTTT ATATCACTGC CATTTTTATG AACATTTGAA TCAGATGGTA 
AAGCATTGTT ACCTAGGACG GTGTATGTTT GATTTGAATT TTTCTGCTTT TGATAGAAAT 
TCAGAAAGCA ATGATTTAAA TGGTTTAGAT GACTCATTCA AGTTTTGGAG GGCTCCATCT 
AGGACAAGTC AGGATCGAGA TCCAAGTTCT CTCTCCACCT CAGAAACAAC GGTGGCACCT 
TCATTGGGGA GTACTGAATT TCAGCCACTT GTGCAGTCAA CAACACTTCT ACCTGAAGCC 
TCCCATGACC AGTTTGTGGC TCAAGGTCAC CAGGAAAGTA CATCACCACG GCCACCTCAT 
GATTCATCTC TTTCTGCTCC CCTGCCCAAA CTGTGTGTTT TTGTTACTCC ATCTCTTCTT 
TCAGCAATGT GCAGCCTCTT GGACAACCTC TTGACGATTG CTCCCAGAGA CACTGCAAAG 
GCTTTTCGAC AAGCTCATCT CATAGAACTT CTCTGTAGCA TTGCAGATGC TACCCTCATA 
CAGACATGTG TCCAGGAACT CAGAGCCCTG CTGCCTTCAT CACCTCCAGC TGAACACACT 
CAGGCTCAGG TTTCCTTTCT CCTGGAATAC CTATCCTCTT TGTCCAGGCT TCTGCAGTCA 
TGTTTATTGG TGGAGCCTGA CCTTGTGATT CAGGATGAGC TTGTGAAACC TCTTATCACC 
AATATCATTG GAATTCTCAC CATATGTACC AAAGATGTAT TAGATAAAGA GTTAATATCA 
GCTTTTTATC ACACATGGAC ACATTTATTT AATCTTCTGG CCATGCTCCT GAGGAAAGCT 
GGTGCCATCA CACTCCCGTT TGTTACCGTG GCCCTGGCCA AGCACTGGAC AGCGGCGATT 
GATATGTTCT GCACATGTGC AGGCTTGTCT GCCACGTGTC CTGCCCTGTA TACTGCCAGC 
TTGCAATTCC TTTCTGTTCT CTTGACCGAA GAAGCAAAAG GGCATCTCCA GGCTAAGAGC 
AAAACACATT TATGCTGTAG TCCAACAGTG GCTTCACTTC TTGATGACTC TCAGGAAAAT 
CAGAAATCTC TAGAACAACT TAGTGATGTA ATCCTTCAGT GCTATGAAGG GAAATCCTCC 
AAAGATATCC TGAAAAGAGT AGCTGCAAAT GCATTGATGT CACTGCTGGC TGTCAGTAGA 
AGAGCACAGA AACATGCTTT GAAAGCCAAT CTTATAGACA ATTGCATGGA GCAGATGAAA 
CACATAAATG CACAACTGAA CCTAGATTCT CTGAGGCCTG GGAAAGCAGC ATTGAAAAAA 
AAGGAGGATG GTGTTATTAA AGAGTTAAGC ATTGCCATGC AGCTCCTAAG AAACTGTCTT 
TATCAAAATG AGGAATGTAA AGAAGCAGCT CTTGAAGCTC ACCTTGTCCC TGTCTTGCAC 
TCTCTCTGGC CTTGGATTTT GATGGATGAT TCATTGATGC AAATTTCTCT GCAGCTCCTT 
TGTGTCTATA CTGCAAATTT TCCAAATGGT TGCAGTTCTC TTTGTTGGTC AAGTTGTGGA 
CAACACCCTG TTCAAGCTAC ACATAGAGGA GCCGTGAGCA ACTCTCTGAT GCTGTGTATC 
CTAAAGTTGG CTTCCCAGAT GCCACTGGAG AACACCACGG TTCAGCAGAT GGTTTTTATG 
CTTCTTTCAA ACCTGGCCTT GTCGCATGAC TGTAAAGGAG TAATTCAGAA GAGTAACTTC 
TTACAGAACT TCCTCTCTCT AGCATTGCCA AAAGGAGGAA ATAAACATCT AAGTAATCTG 
ACTATTCTTT GGTTGAAGTT ACTCCTGAAT ATATCATCTG GAGAAGATGG GCAACAAATG 
ATTCTGAGGC TTGATGGCTG TCTAGACTTA CTAACAGAGA TGAGCAAATA CAAGCACAAG 
AGCAGCCCTT TATTGCCTCT TCTTATCTTT CATAATGTTT GCTTCAGTCC TGCAAATAAA 
CCCAAGATCC TGGCTAATGA AAAAGTCATT ACTGTGCTTG CTGCCTGTCT GGAAAGTGAG 
AATCAAAATG CTCAGAGGAT TGGAGCAGCT GCCCTTTGGG CTCTGATTTA CAATTATCAG 
AAGGCAAAAA CAGCTTTGAA AAGCCCATCA GTAAAAAGAA GAGTGGATGA AGCATACTCC 
TTAGCAAAGA AAACTTTCCC AAACTCAGAA GCAAACCCTC TAAATGCCTA TTATTTGAAA 
TGTCTTGAAA ACCTCGTGCA GCTCCTTAAT TCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881184.1
CDS	191..5809
Translation	MGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_017025695.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025695.1

ORF Insert Sequence:

ATGGGACACA TAGATCTGCC AGAGCTGGAA ACTGAAGACA CATTGGAACT ACAATTCCAG 
CAGCTCAGTC TTCCCCAGTT TTGTGTCTCC ATTCTGGAAT CAGCTGTTCC TCTCTTAAGA 
ACAGGTAGCA GACAAGTGAT AATAAGAGTT CTGGAATTGC TTACAGAGGA TATGACACTT 
ATTGGTGAAG CAATTTCAAC AGATATCTGG GATGACAGCA GCCTTTTTGG TATAGATATG 
AAGGAGAAAC TACTCCTGGT GTTGGGAGCC CTTGGAGAAA CCATGTGCTA CCATAAAAGT 
AGTATCAGTT TGGAGCAGCC AGAGGTGATG CTTGTGCACC ACAGAATGGC CTTTATCAGC 
ATTTCCCTGT TTGCAGTTCG ACTGCTACAA ACGCTTCTCC CTGTTGAAAA GGCAAGCGAG 
TTTTTATCAG AGCCTATGTC AACAGCATTA TTTCTCCTTT CTTTGGACAT GCCTATTTCT 
TTGGAATATC CAAATATTCA TGAAGCTGTT GTGGCCTATT TGGAACAGCT GAATTCTGAA 
AACTACAGTA TTTATAAACG AACTGCAGAG GCCGTTTATT CAATTGAATG CACCTGTAAC 
TTCCTGTCTG ATATTGGGAA AGAGGGAGAG AAGAATCTCT TAGAATTAGT GGAGCTGGCA 
GACCAAGCCT TGCGTAGCTT TTCCTATCAT CAGCATTTCC CGCTAATAAA GGAAATCATC 
AGCATTTGTT CAAAGATCTG GAAATCTGCT CAGGCCAGTC CATTACTACA AGGAGAAAGT 
CAGAAGGTGC TTCTCCATAT GTTGTCTCAC CCATTGCCAC GAGTGAAAGC TGAAACTTAC 
CACTGCTGTC TGGAAATCAC GAAGGAATGT TTAGGTGTCC ATAATGTCAC TAAACCCGTG 
TCTTCACTTT GCAATGGAAT TCATTTTCTA CTTCATCCAA AAGTTCTGTA TGAAATCTCT 
GTATTTGGCA TTCAAGAGCC CGAAAGTGAG CAGGTGAACA CTGCTGCCAA GGCCATTCTG 
TTATACCTGC TTCAGGGACG ATTGATGATG ACAGCATTGA CCTGGAACAA GTTTATTGAA 
TCTCTCTGTC CTGTCATCCC AATACTACAG GGCTATGCCG ACACAGAAGA TCCTCTGGGT 
AACTGCATTC TCCTTCTAAG CAAAGCAAGT TCTGACACAG AAGAGATGCT TCCGTGTACT 
ACCCGATTAA AGTCCATGCT GCGACTTTTG CTAGTGAAAA AGCCATCGGT CCGTTCGCTA 
GCATTAAAGC TTTTAGCATT CCATCTTACC AGTGAAGAAG GGGCTGATAC AAAGCGTCCT 
CTAATAGACG CCAGAGTTCT CTCCAGAGTT ACTGATTTAT TCATTGGGAA GAAACCTATT 
GAACTCAGAC TGGATGACAG AAGAGAGCTG GTTATTAAGT TGGAGACTGT TGAAAAGGTA 
TATGAAATCT TCACCTCAGA TGATGTTGAT CTCGTTTTGA GAAAGTCAGC TGCTGAACAG 
TTAGCTGTGA TTATGCAAGA TATTAAAATG CATGCTGTGG TGAAAAAGTT ATGCTTAATT 
GACAAAATAA TTGAGTATTT AAATGAATGT GTGAGTCAAG ATGGCAAGGT TGTAGAATGT 
TTGGTACAAC CATGCCTCAC ACTCTTGAGG AAGGTTTTAT GTGGTGATCC AGTCATGCGT 
GTTTCGCTCT CGCAACAGTC TTCTCTTTTG ACCGTGTTAT TCAGAGTTTC CTTAATATTT 
CATGAAGATT GTAGTGTCGT GACTGAAGTT GGAGCACTAT TTTGTCTTCT ATTATTTGAT 
GAAGTATCGA GAATGGATAT GTGGTCTGTT AATCCTTCCA ATAAACCTTC TTTGCCATCG 
GTCTTCAGTT TGCCTGTTTC CGTTTTTAGA AGGTACCATC TACCTGTTCA TGTAATTGGA 
CACCATGCTG TGAGTCCTTA CTCCATAGTT TTGCCCTTAT CTGCTGATTG TTTGGCCTTG 
AAGCCGGTGT CAGATATGCT GAGAATAGCT TGGAACCTGT CATGGTATCA TGGGAGTGAT 
AATCTGTTGA AGCAAATGAA CTCTGAAACT AAAACGCAAG AAATTTTAGA TGCATTGAAG 
TTATCTACAG AGGACATCCT CACTCTGAAG ATAACACACA TGGCTAGTGG GCTGCAGGAC 
TGCCTCCATT CCATTGTTCA GGCTGCAACC CACAGGGAAG TTAGGGCTGC TGTCACCAGG 
ATGAGTTTTT ATCTTCTGAA TGACAGATTG TCTTTAAAGG GTTGCCCTGG TCCATGTGGG 
GTTACCTTGA AGTCCTTGGC TTGGCACACC GCACTAAACA GGTTTTTACA GGTGCTTCCT 
GCTTGCACTG AAGATGAGAA ACTGCTAATA GATATCATAC ATTTTTTAAA TAAATTAATA 
AAGGAACAAA GAAAAAATTC TTCACTAGAA CTTCTAAACT GGATTCTCGA ATTACTTCTT 
AGACATAGTG CAAACCCACT GTTAGACCTC TTGGTTCTGA CAGAGTCACA GGCACGAGAA 
GAAACAGATG ATATCCGGAC TGCTGTCAGG CAACAACTTC AGAAAGAACT GATTGCTCTT 
TTTGATACCT TGCTGCTCAA TTTCATGGAA GTTACTGACA GGAAATGCTC GGAACTTCTT 
TACGTTTTTC AAACGCAGCT GGCTCTGAAA CTGCTCCAGT GTCTGAAAGT GACGGATGCG 
CCTCATTTCT ATGGCCTGCC GTCCCTTGAG CGGACCTTAC GAGGGATGGC TAACCTCACT 
GCGTTTCCGG GATGGAGCTC ACACTCTCCT CTCACAAAGC CTCTAGATAT CTGTGTGAAG 
TACTTGTCAG GTCTCCTTGA GGTCATTACT TCTTTTTATG TGGAGCGTGG AGGAAATGCT 
ATGTCCTTCA TGGGAAAAGG TGTTACAAAG AGCACAATTC TTTGCTTGCT TCACTTATCC 
CATGAGATGA TGGCCCAGGC TGGGAGCTTG GAGTGGATGT CACTTTGGTT CTTGCCTTTG 
GGTAGTCATA GTGAAGAACA TATTCCTACT CAACAAGGAT TGGCTTGGTT GATTCCATTA 
TGGGTTGATC GGGACCCAGA GGTGAGATTC ACTTCACTGG GATTAGGATC AGCACTGACC 
ACCCTTGAAA CGGGCTGTGT GGCCTTAGCA AACAGTTGTC AGAACATTTC CGGTGGGCTC 
TGGGGAACTG TGGTGAACAT TCTTCTGGAC CAGTCAGAAT GTAGTATGGT GCGCCGGGAG 
GCGGCATTTA TTCTTCAGAA TCTCCTTGTA ATTCCAATGC CTACAGAAAT TATAAAGGAT 
TATACTTGGC AGGGTCCCTG TGTTCATGAT GAGGACTCTG GCCTATCGCT CATTGGAAAA 
CCTGCCCTTC AGGCTCTTTT ATATCACTGC CATTTTTATG AACATTTGAA TCAGATGGTA 
AAGCATTGTT ACCTAGGACG GTGTATGTTT GATTTGAATT TTTCTGCTTT TGATAGAAAT 
TCAGAAAGCA ATGATTTAAA TGGTTTAGAT GACTCATTCA AGTTTTGGAG GGCTCCATCT 
AGGACAAGTC AGGATCGAGA TCCAAGTTCT CTCTCCACCT CAGAAACAAC GGTGGCACCT 
TCATTGGGGA GTACTGAATT TCAGCCACTT GTGCAGTCAA CAACACTTCT ACCTGAAGCC 
TCCCATGACC AGTTTGTGGC TCAAGGTCAC CAGGAAAGTA CATCACCACG GCCACCTCAT 
GATTCATCTC TTTCTGCTCC CCTGCCCAAA CTGTGTGTTT TTGTTACTCC ATCTCTTCTT 
TCAGCAATGT GCAGCCTCTT GGACAACCTC TTGACGATTG CTCCCAGAGA CACTGCAAAG 
GCTTTTCGAC AAGCTCATCT CATAGAACTT CTCTGTAGCA TTGCAGATGC TACCCTCATA 
CAGACATGTG TCCAGGAACT CAGAGCCCTG CTGCCTTCAT CACCTCCAGC TGAACACACT 
CAGGCTCAGG TTTCCTTTCT CCTGGAATAC CTATCCTCTT TGTCCAGGCT TCTGCAGTCA 
TGTTTATTGG TGGAGCCTGA CCTTGTGATT CAGGATGAGC TTGTGAAACC TCTTATCACC 
AATATCATTG GAATTCTCAC CATATGTACC AAAGATGTAT TAGATAAAGA GTTAATATCA 
GCTTTTTATC ACACATGGAC ACATTTATTT AATCTTCTGG CCATGCTCCT GAGGAAAGCT 
GGTGCCATCA CACTCCCGTT TGTTACCGTG GCCCTGGCCA AGCACTGGAC AGCGGCGATT 
GATATGTTCT GCACATGTGC AGGCTTGTCT GCCACGTGTC CTGCCCTGTA TACTGCCAGC 
TTGCAATTCC TTTCTGTTCT CTTGACCGAA GAAGCAAAAG GGCATCTCCA GGCTAAGAGC 
AAAACACATT TATGCTGTAG TCCAACAGTG GCTTCACTTC TTGATGACTC TCAGGAAAAT 
CAGAAATCTC TAGAACAACT TAGTGATGTA ATCCTTCAGT GCTATGAAGG GAAATCCTCC 
AAAGATATCC TGAAAAGAGT AGCTGCAAAT GCATTGATGT CACTGCTGGC TGTCAGTAGA 
AGAGCACAGA AACATGCTTT GAAAGCCAAT CTTATAGACA ATTGCATGGA GCAGATGAAA 
CACATAAATG CACAACTGAA CCTAGATTCT CTGAGGCCTG GGAAAGCAGC ATTGAAAAAA 
AAGGAGGATG GTGTTATTAA AGAGTTAAGC ATTGCCATGC AGCTCCTAAG AAACTGTCTT 
TATCAAAATG AGGAATGTAA AGAAGCAGCT CTTGAAGCTC ACCTTGTCCC TGTCTTGCAC 
TCTCTCTGGC CTTGGATTTT GATGGATGAT TCATTGATGC AAATTTCTCT GCAGCTCCTT 
TGTGTCTATA CTGCAAATTT TCCAAATGGT TGCAGTTCTC TTTGTTGGTC AAGTTGTGGA 
CAACACCCTG TTCAAGCTAC ACATAGAGGA GCCGTGAGCA ACTCTCTGAT GCTGTGTATC 
CTAAAGTTGG CTTCCCAGAT GCCACTGGAG AACACCACGG TTCAGCAGAT GGTTTTTATG 
CTTCTTTCAA ACCTGGCCTT GTCGCATGAC TGTAAAGGAG TAATTCAGAA GAGTAACTTC 
TTACAGAACT TCCTCTCTCT AGCATTGCCA AAAGGAGGAA ATAAACATCT AAGTAATCTG 
ACTATTCTTT GGTTGAAGTT ACTCCTGAAT ATATCATCTG GAGAAGATGG GCAACAAATG 
ATTCTGAGGC TTGATGGCTG TCTAGACTTA CTAACAGAGA TGAGCAAATA CAAGCACAAG 
AGCAGCCCTT TATTGCCTCT TCTTATCTTT CATAATGTTT GCTTCAGTCC TGCAAATAAA 
CCCAAGATCC TGGCTAATGA AAAAGTCATT ACTGTGCTTG CTGCCTGTCT GGAAAGTGAG 
AATCAAAATG CTCAGAGGAT TGGAGCAGCT GCCCTTTGGG CTCTGATTTA CAATTATCAG 
AAGGCAAAAA CAGCTTTGAA AAGCCCATCA GTAAAAAGAA GAGTGGATGA AGCATACTCC 
TTAGCAAAGA AAACTTTCCC AAACTCAGAA GCAAACCCTC TAAATGCCTA TTATTTGAAA 
TGTCTTGAAA ACCTCGTGCA GCTCCTTAAT TCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525904.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524206.1 Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.

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CloneID	OHu66632
Clone ID Related Accession (Same CDS sequence)	XM_011525904.2 , XM_011525904.3
Accession Version	XM_011525904.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rotatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGAGAGAA CACTGACCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524206.1
CDS	69..6041
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGENTDH

Target ORF information:

RefSeq Version	XM_011525904.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525904.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGAGAGAA CACTGACCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525904.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524206.1 Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.

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CloneID	OHu66632
Clone ID Related Accession (Same CDS sequence)	XM_011525904.2 , XM_011525904.3
Accession Version	XM_011525904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGAGAGAA CACTGACCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524206.1
CDS	69..6041
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGENTDH

Target ORF information:

RefSeq Version	XM_011525904.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525904.3

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGAGAGAA CACTGACCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525905.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524207.1 Homo sapiens rotatin (RTTN), transcript variant X12, mRNA.

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CloneID	OHu66633
Clone ID Related Accession (Same CDS sequence)	XM_011525905.2
Accession Version	XM_011525905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGTGCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524207.1
CDS	69..5402
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIVL

Target ORF information:

RefSeq Version	XM_011525905.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525905.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGTGCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525906.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524208.1 Homo sapiens rotatin (RTTN), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu66634
Clone ID Related Accession (Same CDS sequence)	XM_011525906.2
Accession Version	XM_011525906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524208.1
CDS	850..6033
Translation	MSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_011525906.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525906.2

ORF Insert Sequence:

ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525907.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524209.1 Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu66635
Clone ID Related Accession (Same CDS sequence)	XM_011525907.2
Accession Version	XM_011525907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTATCCTTTT AGTGAAGACA TTCTACCTTC AAAAAGAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524209.1
CDS	69..4862
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGILLVKTFYLQKEK

Target ORF information:

RefSeq Version	XM_011525907.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525907.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTATCCTTTT AGTGAAGACA TTCTACCTTC AAAAAGAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525908.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524210.1 Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.

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CloneID	OHu66636
Clone ID Related Accession (Same CDS sequence)	XM_011525908.2 , XM_011525908.3
Accession Version	XM_011525908.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rotatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGTT 
GCTTCTAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524210.1
CDS	69..4463
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQVASKS

Target ORF information:

RefSeq Version	XM_011525908.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525908.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGTT 
GCTTCTAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525908.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524210.1 Homo sapiens rotatin (RTTN), transcript variant X16, mRNA.

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CloneID	OHu66636
Clone ID Related Accession (Same CDS sequence)	XM_011525908.2 , XM_011525908.3
Accession Version	XM_011525908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGTT 
GCTTCTAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524210.1
CDS	69..4463
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQVASKS

Target ORF information:

RefSeq Version	XM_011525908.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525908.3

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGTT 
GCTTCTAAAA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_017025693.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881182.1 Homo sapiens rotatin (RTTN), transcript variant X3, mRNA.

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CloneID	OHu87841
Clone ID Related Accession (Same CDS sequence)	XM_017025693.1
Accession Version	XM_017025693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881182.1
CDS	69..6509
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_017025693.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025693.1

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_017025694.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881183.1 Homo sapiens rotatin (RTTN), transcript variant X6, mRNA.

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CloneID	OHu87842
Clone ID Related Accession (Same CDS sequence)	XM_017025694.1
Accession Version	XM_017025694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGATT TTCCTGCTGA GATTTTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
TCTCTGTTGA AACTGGCCTT TGGAGATGGA AAGCATCGCC TGGCATTACA GTCGGTGTCC 
TGCCTGCAGC AGCTGTGCAT GTATTTAAGA AACAGACTTA ACTTTCACCG AGATCCAGGT 
TTTTTCTCCA ATAAACACGA CACAGTTTCC CAAAATTCTT CTTTGTCTTA TTGTCATGAA 
GCAAGAGGTA CTCATCATTC CCAGAATCCT TCCCCAGGAA GCAGCAGCCC TCGGCCTTCT 
GTGGTTGGGC GCACAGGCCA GCGACCCAGA GGAGATGGCC AGGACTGGGA TGCAGCGTCC 
TCTAGTGGAA GTAGTAGTCA TGCTCATGTA AACTCCAGGA TATCCGTTCA TTCACCTTTG 
GATATGGGAC ACATAGATCT GCCAGAGCTG GAAACTGAAG ACACATTGGA ACTACAATTC 
CAGCAGCTCA GTCTTCCCCA GTTTTGTGTC TCCATTCTGG AATCAGCTGT TCCTCTCTTA 
AGAACAGGTA GCAGACAAGT GATAATAAGA GTTCTGGAAT TGCTTACAGA GGATATGACA 
CTTATTGGTG AAGCAATTTC AACAGATATC TGGGATGACA GCAGCCTTTT TGGTATAGAT 
ATGAAGGAGA AACTACTCCT GGTGTTGGGA GCCCTTGGAG AAACCATGTG CTACCATAAA 
AGTAGTATCA GTTTGGAGCA GCCAGAGGTG ATGCTTGTGC ACCACAGAAT GGCCTTTATC 
AGCATTTCCC TGTTTGCAGT TCGACTGCTA CAAACGCTTC TCCCTGTTGA AAAGGCAAGC 
GAGTTTTTAT CAGAGCCTAT GTCAACAGCA TTATTTCTCC TTTCTTTGGA CATGCCTATT 
TCTTTGGAAT ATCCAAATAT TCATGAAGCT GTTGTGGCCT ATTTGGAACA GCTGAATTCT 
GAAAACTACA GTATTTATAA ACGAACTGCA GAGGCCGTTT ATTCAATTGA ATGCACCTGT 
AACTTCCTGT CTGATATTGG GAAAGAGGGA GAGAAGAATC TCTTAGAATT AGTGGAGCTG 
GCAGACCAAG CCTTGCGTAG CTTTTCCTAT CATCAGCATT TCCCGCTAAT AAAGGAAATC 
ATCAGCATTT GTTCAAAGAT CTGGAAATCT GCTCAGGCCA GTCCATTACT ACAAGGAGAA 
AGTCAGAAGG TGCTTCTCCA TATGTTGTCT CACCCATTGC CACGAGTGAA AGCTGAAACT 
TACCACTGCT GTCTGGAAAT CACGAAGGAA TGTTTAGGTG TCCATAATGT CACTAAACCC 
GTGTCTTCAC TTTGCAATGG AATTCATTTT CTACTTCATC CAAAAGTTCT GTATGAAATC 
TCTGTATTTG GCATTCAAGA GCCCGAAAGT GAGCAGGTGA ACACTGCTGC CAAGGCCATT 
CTGTTATACC TGCTTCAGGG ACGATTGATG ATGACAGCAT TGACCTGGAA CAAGTTTATT 
GAATCTCTCT GTCCTGTCAT CCCAATACTA CAGGGCTATG CCGACACAGA AGATCCTCTG 
GGTAACTGCA TTCTCCTTCT AAGCAAAGCA AGTTCTGACA CAGAAGAGAT GCTTCCGTGT 
ACTACCCGAT TAAAGTCCAT GCTGCGACTT TTGCTAGTGA AAAAGCCATC GGTCCGTTCG 
CTAGCATTAA AGCTTTTAGC ATTCCATCTT ACCAGTGAAG AAGGGGCTGA TACAAAGCGT 
CCTCTAATAG ACGCCAGAGT TCTCTCCAGA GTTACTGATT TATTCATTGG GAAGAAACCT 
ATTGAACTCA GACTGGATGA CAGAAGAGAG CTGGTTATTA AGTTGGAGAC TGTTGAAAAG 
GTATATGAAA TCTTCACCTC AGATGATGTT GATCTCGTTT TGAGAAAGTC AGCTGCTGAA 
CAGTTAGCTG TGATTATGCA AGATATTAAA ATGCATGCTG TGGTGAAAAA GTTATGCTTA 
ATTGACAAAA TAATTGAGTA TTTAAATGAA TGTGTGAGTC AAGATGGCAA GGTTGTAGAA 
TGTTTGGTAC AACCATGCCT CACACTCTTG AGGAAGGTTT TATGTGGTGA TCCAGTCATG 
CGTGTTTCGC TCTCGCAACA GTCTTCTCTT TTGACCGTGT TATTCAGAGT TTCCTTAATA 
TTTCATGAAG ATTGTAGTGT CGTGACTGAA GTTGGAGCAC TATTTTGTCT TCTATTATTT 
GATGAAGTAT CGAGAATGGA TATGTGGTCT GTTAATCCTT CCAATAAACC TTCTTTGCCA 
TCGGTCTTCA GTTTGCCTGT TTCCGTTTTT AGAAGGTACC ATCTACCTGT TCATGTAATT 
GGACACCATG CTGTGAGTCC TTACTCCATA GTTTTGCCCT TATCTGCTGA TTGTTTGGCC 
TTGAAGCCGG TGTCAGATAT GCTGAGAATA GCTTGGAACC TGTCATGGTA TCATGGGAGT 
GATAATCTGT TGAAGCAAAT GAACTCTGAA ACTAAAACGC AAGAAATTTT AGATGCATTG 
AAGTTATCTA CAGAGGACAT CCTCACTCTG AAGATAACAC ACATGGCTAG TGGGCTGCAG 
GACTGCCTCC ATTCCATTGT TCAGGCTGCA ACCCACAGGG AAGTTAGGGC TGCTGTCACC 
AGGATGAGTT TTTATCTTCT GAATGACAGA TTGTCTTTAA AGGGTTGCCC TGGTCCATGT 
GGGGTTACCT TGAAGTCCTT GGCTTGGCAC ACCGCACTAA ACAGGTTTTT ACAGGTGCTT 
CCTGCTTGCA CTGAAGATGA GAAACTGCTA ATAGATATCA TACATTTTTT AAATAAATTA 
ATAAAGGAAC AAAGAAAAAA TTCTTCACTA GAACTTCTAA ACTGGATTCT CGAATTACTT 
CTTAGACATA GTGCAAACCC ACTGTTAGAC CTCTTGGTTC TGACAGAGTC ACAGGCACGA 
GAAGAAACAG ATGATATCCG GACTGCTGTC AGGCAACAAC TTCAGAAAGA ACTGATTGCT 
CTTTTTGATA CCTTGCTGCT CAATTTCATG GAAGTTACTG ACAGGAAATG CTCGGAACTT 
CTTTACGTTT TTCAAACGCA GCTGGCTCTG AAACTGCTCC AGTGTCTGAA AGTGACGGAT 
GCGCCTCATT TCTATGGCCT GCCGTCCCTT GAGCGGACCT TACGAGGGAT GGCTAACCTC 
ACTGCGTTTC CGGGATGGAG CTCACACTCT CCTCTCACAA AGCCTCTAGA TATCTGTGTG 
AAGTACTTGT CAGGTCTCCT TGAGGTCATT ACTTCTTTTT ATGTGGAGCG TGGAGGAAAT 
GCTATGTCCT TCATGGGAAA AGGTGTTACA AAGAGCACAA TTCTTTGCTT GCTTCACTTA 
TCCCATGAGA TGATGGCCCA GGCTGGGAGC TTGGAGTGGA TGTCACTTTG GTTCTTGCCT 
TTGGGTAGTC ATAGTGAAGA ACATATTCCT ACTCAACAAG GATTGGCTTG GTTGATTCCA 
TTATGGGTTG ATCGGGACCC AGAGGTGAGA TTCACTTCAC TGGGATTAGG ATCAGCACTG 
ACCACCCTTG AAACGGGCTG TGTGGCCTTA GCAAACAGTT GTCAGAACAT TTCCGGTGGG 
CTCTGGGGAA CTGTGGTGAA CATTCTTCTG GACCAGTCAG AATGTAGTAT GGTGCGCCGG 
GAGGCGGCAT TTATTCTTCA GAATCTCCTT GTAATTCCAA TGCCTACAGA AATTATAAAG 
GATTATACTT GGCAGGGTCC CTGTGTTCAT GATGAGGACT CTGGCCTATC GCTCATTGGA 
AAACCTGCCC TTCAGGCTCT TTTATATCAC TGCCATTTTT ATGAACATTT GAATCAGATG 
GTAAAGCATT GTTACCTAGG ACGGTGTATG TTTGATTTGA ATTTTTCTGC TTTTGATAGA 
AATTCAGAAA GCAATGATTT AAATGGTTTA GATGACTCAT TCAAGTTTTG GAGGGCTCCA 
TCTAGGACAA GTCAGGATCG AGATCCAAGT TCTCTCTCCA CCTCAGAAAC AACGGTGGCA 
CCTTCATTGG GGAGTACTGA ATTTCAGCCA CTTGTGCAGT CAACAACACT TCTACCTGAA 
GCCTCCCATG ACCAGTTTGT GGCTCAAGGT CACCAGGAAA GTACATCACC ACGGCCACCT 
CATGATTCAT CTCTTTCTGC TCCCCTGCCC AAACTGTGTG TTTTTGTTAC TCCATCTCTT 
CTTTCAGCAA TGTGCAGCCT CTTGGACAAC CTCTTGACGA TTGCTCCCAG AGACACTGCA 
AAGGCTTTTC GACAAGCTCA TCTCATAGAA CTTCTCTGTA GCATTGCAGA TGCTACCCTC 
ATACAGACAT GTGTCCAGGA ACTCAGAGCC CTGCTGCCTT CATCACCTCC AGCTGAACAC 
ACTCAGGCTC AGGTTTCCTT TCTCCTGGAA TACCTATCCT CTTTGTCCAG GCTTCTGCAG 
TCATGTTTAT TGGTGGAGCC TGACCTTGTG ATTCAGGATG AGCTTGTGAA ACCTCTTATC 
ACCAATATCA TTGGAATTCT CACCATATGT ACCAAAGATG TATTAGATAA AGAGTTAATA 
TCAGCTTTTT ATCACACATG GACACATTTA TTTAATCTTC TGGCCATGCT CCTGAGGAAA 
GCTGGTGCCA TCACACTCCC GTTTGTTACC GTGGCCCTGG CCAAGCACTG GACAGCGGCG 
ATTGATATGT TCTGCACATG TGCAGGCTTG TCTGCCACGT GTCCTGCCCT GTATACTGCC 
AGCTTGCAAT TCCTTTCTGT TCTCTTGACC GAAGAAGCAA AAGGGCATCT CCAGGCTAAG 
AGCAAAACAC ATTTATGCTG TAGTCCAACA GTGGCTTCAC TTCTTGATGA CTCTCAGGAA 
AATCAGAAAT CTCTAGAACA ACTTAGTGAT GTAATCCTTC AGTGCTATGA AGGGAAATCC 
TCCAAAGATA TCCTGAAAAG AGTAGCTGCA AATGCATTGA TGTCACTGCT GGCTGTCAGT 
AGAAGAGCAC AGAAACATGC TTTGAAAGCC AATCTTATAG ACAATTGCAT GGAGCAGATG 
AAACACATAA ATGCACAACT GAACCTAGAT TCTCTGAGGC CTGGGAAAGC AGCATTGAAA 
AAAAAGGAGG ATGGTGTTAT TAAAGAGTTA AGCATTGCCA TGCAGCTCCT AAGAAACTGT 
CTTTATCAAA ATGAGGAATG TAAAGAAGCA GCTCTTGAAG CTCACCTTGT CCCTGTCTTG 
CACTCTCTCT GGCCTTGGAT TTTGATGGAT GATTCATTGA TGCAAATTTC TCTGCAGCTC 
CTTTGTGTCT ATACTGCAAA TTTTCCAAAT GGTTGCAGTT CTCTTTGTTG GTCAAGTTGT 
GGACAACACC CTGTTCAAGC TACACATAGA GGAGCCGTGA GCAACTCTCT GATGCTGTGT 
ATCCTAAAGT TGGCTTCCCA GATGCCACTG GAGAACACCA CGGTTCAGCA GATGGTTTTT 
ATGCTTCTTT CAAACCTGGC CTTGTCGCAT GACTGTAAAG GAGTAATTCA GAAGAGTAAC 
TTCTTACAGA ACTTCCTCTC TCTAGCATTG CCAAAAGGAG GAAATAAACA TCTAAGTAAT 
CTGACTATTC TTTGGTTGAA GTTACTCCTG AATATATCAT CTGGAGAAGA TGGGCAACAA 
ATGATTCTGA GGCTTGATGG CTGTCTAGAC TTACTAACAG AGATGAGCAA ATACAAGCAC 
AAGAGCAGCC CTTTATTGCC TCTTCTTATC TTTCATAATG TTTGCTTCAG TCCTGCAAAT 
AAACCCAAGA TCCTGGCTAA TGAAAAAGTC ATTACTGTGC TTGCTGCCTG TCTGGAAAGT 
GAGAATCAAA ATGCTCAGAG GATTGGAGCA GCTGCCCTTT GGGCTCTGAT TTACAATTAT 
CAGAAGGCAA AAACAGCTTT GAAAAGCCCA TCAGTAAAAA GAAGAGTGGA TGAAGCATAC 
TCCTTAGCAA AGAAAACTTT CCCAAACTCA GAAGCAAACC CTCTAAATGC CTATTATTTG 
AAATGTCTTG AAAACCTCGT GCAGCTCCTT AATTCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881183.1
CDS	266..6307
Translation	MQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_017025694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025694.1

ORF Insert Sequence:

ATGCAAGATT TTCCTGCTGA GATTTTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
TCTCTGTTGA AACTGGCCTT TGGAGATGGA AAGCATCGCC TGGCATTACA GTCGGTGTCC 
TGCCTGCAGC AGCTGTGCAT GTATTTAAGA AACAGACTTA ACTTTCACCG AGATCCAGGT 
TTTTTCTCCA ATAAACACGA CACAGTTTCC CAAAATTCTT CTTTGTCTTA TTGTCATGAA 
GCAAGAGGTA CTCATCATTC CCAGAATCCT TCCCCAGGAA GCAGCAGCCC TCGGCCTTCT 
GTGGTTGGGC GCACAGGCCA GCGACCCAGA GGAGATGGCC AGGACTGGGA TGCAGCGTCC 
TCTAGTGGAA GTAGTAGTCA TGCTCATGTA AACTCCAGGA TATCCGTTCA TTCACCTTTG 
GATATGGGAC ACATAGATCT GCCAGAGCTG GAAACTGAAG ACACATTGGA ACTACAATTC 
CAGCAGCTCA GTCTTCCCCA GTTTTGTGTC TCCATTCTGG AATCAGCTGT TCCTCTCTTA 
AGAACAGGTA GCAGACAAGT GATAATAAGA GTTCTGGAAT TGCTTACAGA GGATATGACA 
CTTATTGGTG AAGCAATTTC AACAGATATC TGGGATGACA GCAGCCTTTT TGGTATAGAT 
ATGAAGGAGA AACTACTCCT GGTGTTGGGA GCCCTTGGAG AAACCATGTG CTACCATAAA 
AGTAGTATCA GTTTGGAGCA GCCAGAGGTG ATGCTTGTGC ACCACAGAAT GGCCTTTATC 
AGCATTTCCC TGTTTGCAGT TCGACTGCTA CAAACGCTTC TCCCTGTTGA AAAGGCAAGC 
GAGTTTTTAT CAGAGCCTAT GTCAACAGCA TTATTTCTCC TTTCTTTGGA CATGCCTATT 
TCTTTGGAAT ATCCAAATAT TCATGAAGCT GTTGTGGCCT ATTTGGAACA GCTGAATTCT 
GAAAACTACA GTATTTATAA ACGAACTGCA GAGGCCGTTT ATTCAATTGA ATGCACCTGT 
AACTTCCTGT CTGATATTGG GAAAGAGGGA GAGAAGAATC TCTTAGAATT AGTGGAGCTG 
GCAGACCAAG CCTTGCGTAG CTTTTCCTAT CATCAGCATT TCCCGCTAAT AAAGGAAATC 
ATCAGCATTT GTTCAAAGAT CTGGAAATCT GCTCAGGCCA GTCCATTACT ACAAGGAGAA 
AGTCAGAAGG TGCTTCTCCA TATGTTGTCT CACCCATTGC CACGAGTGAA AGCTGAAACT 
TACCACTGCT GTCTGGAAAT CACGAAGGAA TGTTTAGGTG TCCATAATGT CACTAAACCC 
GTGTCTTCAC TTTGCAATGG AATTCATTTT CTACTTCATC CAAAAGTTCT GTATGAAATC 
TCTGTATTTG GCATTCAAGA GCCCGAAAGT GAGCAGGTGA ACACTGCTGC CAAGGCCATT 
CTGTTATACC TGCTTCAGGG ACGATTGATG ATGACAGCAT TGACCTGGAA CAAGTTTATT 
GAATCTCTCT GTCCTGTCAT CCCAATACTA CAGGGCTATG CCGACACAGA AGATCCTCTG 
GGTAACTGCA TTCTCCTTCT AAGCAAAGCA AGTTCTGACA CAGAAGAGAT GCTTCCGTGT 
ACTACCCGAT TAAAGTCCAT GCTGCGACTT TTGCTAGTGA AAAAGCCATC GGTCCGTTCG 
CTAGCATTAA AGCTTTTAGC ATTCCATCTT ACCAGTGAAG AAGGGGCTGA TACAAAGCGT 
CCTCTAATAG ACGCCAGAGT TCTCTCCAGA GTTACTGATT TATTCATTGG GAAGAAACCT 
ATTGAACTCA GACTGGATGA CAGAAGAGAG CTGGTTATTA AGTTGGAGAC TGTTGAAAAG 
GTATATGAAA TCTTCACCTC AGATGATGTT GATCTCGTTT TGAGAAAGTC AGCTGCTGAA 
CAGTTAGCTG TGATTATGCA AGATATTAAA ATGCATGCTG TGGTGAAAAA GTTATGCTTA 
ATTGACAAAA TAATTGAGTA TTTAAATGAA TGTGTGAGTC AAGATGGCAA GGTTGTAGAA 
TGTTTGGTAC AACCATGCCT CACACTCTTG AGGAAGGTTT TATGTGGTGA TCCAGTCATG 
CGTGTTTCGC TCTCGCAACA GTCTTCTCTT TTGACCGTGT TATTCAGAGT TTCCTTAATA 
TTTCATGAAG ATTGTAGTGT CGTGACTGAA GTTGGAGCAC TATTTTGTCT TCTATTATTT 
GATGAAGTAT CGAGAATGGA TATGTGGTCT GTTAATCCTT CCAATAAACC TTCTTTGCCA 
TCGGTCTTCA GTTTGCCTGT TTCCGTTTTT AGAAGGTACC ATCTACCTGT TCATGTAATT 
GGACACCATG CTGTGAGTCC TTACTCCATA GTTTTGCCCT TATCTGCTGA TTGTTTGGCC 
TTGAAGCCGG TGTCAGATAT GCTGAGAATA GCTTGGAACC TGTCATGGTA TCATGGGAGT 
GATAATCTGT TGAAGCAAAT GAACTCTGAA ACTAAAACGC AAGAAATTTT AGATGCATTG 
AAGTTATCTA CAGAGGACAT CCTCACTCTG AAGATAACAC ACATGGCTAG TGGGCTGCAG 
GACTGCCTCC ATTCCATTGT TCAGGCTGCA ACCCACAGGG AAGTTAGGGC TGCTGTCACC 
AGGATGAGTT TTTATCTTCT GAATGACAGA TTGTCTTTAA AGGGTTGCCC TGGTCCATGT 
GGGGTTACCT TGAAGTCCTT GGCTTGGCAC ACCGCACTAA ACAGGTTTTT ACAGGTGCTT 
CCTGCTTGCA CTGAAGATGA GAAACTGCTA ATAGATATCA TACATTTTTT AAATAAATTA 
ATAAAGGAAC AAAGAAAAAA TTCTTCACTA GAACTTCTAA ACTGGATTCT CGAATTACTT 
CTTAGACATA GTGCAAACCC ACTGTTAGAC CTCTTGGTTC TGACAGAGTC ACAGGCACGA 
GAAGAAACAG ATGATATCCG GACTGCTGTC AGGCAACAAC TTCAGAAAGA ACTGATTGCT 
CTTTTTGATA CCTTGCTGCT CAATTTCATG GAAGTTACTG ACAGGAAATG CTCGGAACTT 
CTTTACGTTT TTCAAACGCA GCTGGCTCTG AAACTGCTCC AGTGTCTGAA AGTGACGGAT 
GCGCCTCATT TCTATGGCCT GCCGTCCCTT GAGCGGACCT TACGAGGGAT GGCTAACCTC 
ACTGCGTTTC CGGGATGGAG CTCACACTCT CCTCTCACAA AGCCTCTAGA TATCTGTGTG 
AAGTACTTGT CAGGTCTCCT TGAGGTCATT ACTTCTTTTT ATGTGGAGCG TGGAGGAAAT 
GCTATGTCCT TCATGGGAAA AGGTGTTACA AAGAGCACAA TTCTTTGCTT GCTTCACTTA 
TCCCATGAGA TGATGGCCCA GGCTGGGAGC TTGGAGTGGA TGTCACTTTG GTTCTTGCCT 
TTGGGTAGTC ATAGTGAAGA ACATATTCCT ACTCAACAAG GATTGGCTTG GTTGATTCCA 
TTATGGGTTG ATCGGGACCC AGAGGTGAGA TTCACTTCAC TGGGATTAGG ATCAGCACTG 
ACCACCCTTG AAACGGGCTG TGTGGCCTTA GCAAACAGTT GTCAGAACAT TTCCGGTGGG 
CTCTGGGGAA CTGTGGTGAA CATTCTTCTG GACCAGTCAG AATGTAGTAT GGTGCGCCGG 
GAGGCGGCAT TTATTCTTCA GAATCTCCTT GTAATTCCAA TGCCTACAGA AATTATAAAG 
GATTATACTT GGCAGGGTCC CTGTGTTCAT GATGAGGACT CTGGCCTATC GCTCATTGGA 
AAACCTGCCC TTCAGGCTCT TTTATATCAC TGCCATTTTT ATGAACATTT GAATCAGATG 
GTAAAGCATT GTTACCTAGG ACGGTGTATG TTTGATTTGA ATTTTTCTGC TTTTGATAGA 
AATTCAGAAA GCAATGATTT AAATGGTTTA GATGACTCAT TCAAGTTTTG GAGGGCTCCA 
TCTAGGACAA GTCAGGATCG AGATCCAAGT TCTCTCTCCA CCTCAGAAAC AACGGTGGCA 
CCTTCATTGG GGAGTACTGA ATTTCAGCCA CTTGTGCAGT CAACAACACT TCTACCTGAA 
GCCTCCCATG ACCAGTTTGT GGCTCAAGGT CACCAGGAAA GTACATCACC ACGGCCACCT 
CATGATTCAT CTCTTTCTGC TCCCCTGCCC AAACTGTGTG TTTTTGTTAC TCCATCTCTT 
CTTTCAGCAA TGTGCAGCCT CTTGGACAAC CTCTTGACGA TTGCTCCCAG AGACACTGCA 
AAGGCTTTTC GACAAGCTCA TCTCATAGAA CTTCTCTGTA GCATTGCAGA TGCTACCCTC 
ATACAGACAT GTGTCCAGGA ACTCAGAGCC CTGCTGCCTT CATCACCTCC AGCTGAACAC 
ACTCAGGCTC AGGTTTCCTT TCTCCTGGAA TACCTATCCT CTTTGTCCAG GCTTCTGCAG 
TCATGTTTAT TGGTGGAGCC TGACCTTGTG ATTCAGGATG AGCTTGTGAA ACCTCTTATC 
ACCAATATCA TTGGAATTCT CACCATATGT ACCAAAGATG TATTAGATAA AGAGTTAATA 
TCAGCTTTTT ATCACACATG GACACATTTA TTTAATCTTC TGGCCATGCT CCTGAGGAAA 
GCTGGTGCCA TCACACTCCC GTTTGTTACC GTGGCCCTGG CCAAGCACTG GACAGCGGCG 
ATTGATATGT TCTGCACATG TGCAGGCTTG TCTGCCACGT GTCCTGCCCT GTATACTGCC 
AGCTTGCAAT TCCTTTCTGT TCTCTTGACC GAAGAAGCAA AAGGGCATCT CCAGGCTAAG 
AGCAAAACAC ATTTATGCTG TAGTCCAACA GTGGCTTCAC TTCTTGATGA CTCTCAGGAA 
AATCAGAAAT CTCTAGAACA ACTTAGTGAT GTAATCCTTC AGTGCTATGA AGGGAAATCC 
TCCAAAGATA TCCTGAAAAG AGTAGCTGCA AATGCATTGA TGTCACTGCT GGCTGTCAGT 
AGAAGAGCAC AGAAACATGC TTTGAAAGCC AATCTTATAG ACAATTGCAT GGAGCAGATG 
AAACACATAA ATGCACAACT GAACCTAGAT TCTCTGAGGC CTGGGAAAGC AGCATTGAAA 
AAAAAGGAGG ATGGTGTTAT TAAAGAGTTA AGCATTGCCA TGCAGCTCCT AAGAAACTGT 
CTTTATCAAA ATGAGGAATG TAAAGAAGCA GCTCTTGAAG CTCACCTTGT CCCTGTCTTG 
CACTCTCTCT GGCCTTGGAT TTTGATGGAT GATTCATTGA TGCAAATTTC TCTGCAGCTC 
CTTTGTGTCT ATACTGCAAA TTTTCCAAAT GGTTGCAGTT CTCTTTGTTG GTCAAGTTGT 
GGACAACACC CTGTTCAAGC TACACATAGA GGAGCCGTGA GCAACTCTCT GATGCTGTGT 
ATCCTAAAGT TGGCTTCCCA GATGCCACTG GAGAACACCA CGGTTCAGCA GATGGTTTTT 
ATGCTTCTTT CAAACCTGGC CTTGTCGCAT GACTGTAAAG GAGTAATTCA GAAGAGTAAC 
TTCTTACAGA ACTTCCTCTC TCTAGCATTG CCAAAAGGAG GAAATAAACA TCTAAGTAAT 
CTGACTATTC TTTGGTTGAA GTTACTCCTG AATATATCAT CTGGAGAAGA TGGGCAACAA 
ATGATTCTGA GGCTTGATGG CTGTCTAGAC TTACTAACAG AGATGAGCAA ATACAAGCAC 
AAGAGCAGCC CTTTATTGCC TCTTCTTATC TTTCATAATG TTTGCTTCAG TCCTGCAAAT 
AAACCCAAGA TCCTGGCTAA TGAAAAAGTC ATTACTGTGC TTGCTGCCTG TCTGGAAAGT 
GAGAATCAAA ATGCTCAGAG GATTGGAGCA GCTGCCCTTT GGGCTCTGAT TTACAATTAT 
CAGAAGGCAA AAACAGCTTT GAAAAGCCCA TCAGTAAAAA GAAGAGTGGA TGAAGCATAC 
TCCTTAGCAA AGAAAACTTT CCCAAACTCA GAAGCAAACC CTCTAAATGC CTATTATTTG 
AAATGTCTTG AAAACCTCGT GCAGCTCCTT AATTCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( NM_173630.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_775901.3 Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.

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CloneID	OHu19540
Clone ID Related Accession (Same CDS sequence)	NM_173630.4 , NM_173630.3
Accession Version	NM_173630.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	rotatin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096404.1, BF507420.1, BC047602.1, BC046222.2 and AC011930.5. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_173630.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000640769.2/ ENSP00000491507.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAAATTTTA GATGCATTGA AGTTATCTAC AGAGGACATC 
CTCACTCTGA AGATAACACA CATGGCTAGT GGGCTGCAGG ACTGCCTCCA TTCCATTGTT 
CAGGCTGCAA CCCACAGGGA AGTTAGGGCT GCTGTCACCA GGATGAGTTT TTATCTTCTG 
AATGACAGAT TGTCTTTAAA GGGTTGCCCT GGTCCATGTG GGGTTACCTT GAAGTCCTTG 
GCTTGGCACA CCGCACTAAA CAGGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775901.3
CDS	30..6710
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	NM_173630.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173630.4

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAAATTTTA GATGCATTGA AGTTATCTAC AGAGGACATC 
CTCACTCTGA AGATAACACA CATGGCTAGT GGGCTGCAGG ACTGCCTCCA TTCCATTGTT 
CAGGCTGCAA CCCACAGGGA AGTTAGGGCT GCTGTCACCA GGATGAGTTT TTATCTTCTG 
AATGACAGAT TGTCTTTAAA GGGTTGCCCT GGTCCATGTG GGGTTACCTT GAAGTCCTTG 
GCTTGGCACA CCGCACTAAA CAGGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( NM_173630.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775901.3 Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.

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CloneID	OHu19540
Clone ID Related Accession (Same CDS sequence)	NM_173630.4 , NM_173630.3
Accession Version	NM_173630.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	rotatin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096404.1, BF507420.1, BC047602.1 and BC046222.2. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2007 this sequence version replaced NM_173630.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAAATTTTA GATGCATTGA AGTTATCTAC AGAGGACATC 
CTCACTCTGA AGATAACACA CATGGCTAGT GGGCTGCAGG ACTGCCTCCA TTCCATTGTT 
CAGGCTGCAA CCCACAGGGA AGTTAGGGCT GCTGTCACCA GGATGAGTTT TTATCTTCTG 
AATGACAGAT TGTCTTTAAA GGGTTGCCCT GGTCCATGTG GGGTTACCTT GAAGTCCTTG 
GCTTGGCACA CCGCACTAAA CAGGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775901.3
CDS	69..6749
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	NM_173630.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173630.3

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGGTGAA CACTGCTGCC AAGGCCATTC TGTTATACCT GCTTCAGGGA 
CGATTGATGA TGACAGCATT GACCTGGAAC AAGTTTATTG AATCTCTCTG TCCTGTCATC 
CCAATACTAC AGGGCTATGC CGACACAGAA GATCCTCTGG GTAACTGCAT TCTCCTTCTA 
AGCAAAGCAA GTTCTGACAC AGAAGAGATG CTTCCGTGTA CTACCCGATT AAAGTCCATG 
CTGCGACTTT TGCTAGTGAA AAAGCCATCG GTCCGTTCGC TAGCATTAAA GCTTTTAGCA 
TTCCATCTTA CCAGTGAAGA AGGGGCTGAT ACAAAGCGTC CTCTAATAGA CGCCAGAGTT 
CTCTCCAGAG TTACTGATTT ATTCATTGGG AAGAAACCTA TTGAACTCAG ACTGGATGAC 
AGAAGAGAGC TGGTTATTAA GTTGGAGACT GTTGAAAAGG TATATGAAAT CTTCACCTCA 
GATGATGTTG ATCTCGTTTT GAGAAAGTCA GCTGCTGAAC AGTTAGCTGT GATTATGCAA 
GATATTAAAA TGCATGCTGT GGTGAAAAAG TTATGCTTAA TTGACAAAAT AATTGAGTAT 
TTAAATGAAT GTGTGAGTCA AGATGGCAAG GTTGTAGAAT GTTTGGTACA ACCATGCCTC 
ACACTCTTGA GGAAGGTTTT ATGTGGTGAT CCAGTCATGC GTGTTTCGCT CTCGCAACAG 
TCTTCTCTTT TGACCGTGTT ATTCAGAGTT TCCTTAATAT TTCATGAAGA TTGTAGTGTC 
GTGACTGAAG TTGGAGCACT ATTTTGTCTT CTATTATTTG ATGAAGTATC GAGAATGGAT 
ATGTGGTCTG TTAATCCTTC CAATAAACCT TCTTTGCCAT CGGTCTTCAG TTTGCCTGTT 
TCCGTTTTTA GAAGGTACCA TCTACCTGTT CATGTAATTG GACACCATGC TGTGAGTCCT 
TACTCCATAG TTTTGCCCTT ATCTGCTGAT TGTTTGGCCT TGAAGCCGGT GTCAGATATG 
CTGAGAATAG CTTGGAACCT GTCATGGTAT CATGGGAGTG ATAATCTGTT GAAGCAAATG 
AACTCTGAAA CTAAAACGCA AGAAATTTTA GATGCATTGA AGTTATCTAC AGAGGACATC 
CTCACTCTGA AGATAACACA CATGGCTAGT GGGCTGCAGG ACTGCCTCCA TTCCATTGTT 
CAGGCTGCAA CCCACAGGGA AGTTAGGGCT GCTGTCACCA GGATGAGTTT TTATCTTCTG 
AATGACAGAT TGTCTTTAAA GGGTTGCCCT GGTCCATGTG GGGTTACCTT GAAGTCCTTG 
GCTTGGCACA CCGCACTAAA CAGGTTTTTA CAGGTGCTTC CTGCTTGCAC TGAAGATGAG 
AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAATTAA TAAAGGAACA AAGAAAAAAT 
TCTTCACTAG AACTTCTAAA CTGGATTCTC GAATTACTTC TTAGACATAG TGCAAACCCA 
CTGTTAGACC TCTTGGTTCT GACAGAGTCA CAGGCACGAG AAGAAACAGA TGATATCCGG 
ACTGCTGTCA GGCAACAACT TCAGAAAGAA CTGATTGCTC TTTTTGATAC CTTGCTGCTC 
AATTTCATGG AAGTTACTGA CAGGAAATGC TCGGAACTTC TTTACGTTTT TCAAACGCAG 
CTGGCTCTGA AACTGCTCCA GTGTCTGAAA GTGACGGATG CGCCTCATTT CTATGGCCTG 
CCGTCCCTTG AGCGGACCTT ACGAGGGATG GCTAACCTCA CTGCGTTTCC GGGATGGAGC 
TCACACTCTC CTCTCACAAA GCCTCTAGAT ATCTGTGTGA AGTACTTGTC AGGTCTCCTT 
GAGGTCATTA CTTCTTTTTA TGTGGAGCGT GGAGGAAATG CTATGTCCTT CATGGGAAAA 
GGTGTTACAA AGAGCACAAT TCTTTGCTTG CTTCACTTAT CCCATGAGAT GATGGCCCAG 
GCTGGGAGCT TGGAGTGGAT GTCACTTTGG TTCTTGCCTT TGGGTAGTCA TAGTGAAGAA 
CATATTCCTA CTCAACAAGG ATTGGCTTGG TTGATTCCAT TATGGGTTGA TCGGGACCCA 
GAGGTGAGAT TCACTTCACT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACGGGCTGT 
GTGGCCTTAG CAAACAGTTG TCAGAACATT TCCGGTGGGC TCTGGGGAAC TGTGGTGAAC 
ATTCTTCTGG ACCAGTCAGA ATGTAGTATG GTGCGCCGGG AGGCGGCATT TATTCTTCAG 
AATCTCCTTG TAATTCCAAT GCCTACAGAA ATTATAAAGG ATTATACTTG GCAGGGTCCC 
TGTGTTCATG ATGAGGACTC TGGCCTATCG CTCATTGGAA AACCTGCCCT TCAGGCTCTT 
TTATATCACT GCCATTTTTA TGAACATTTG AATCAGATGG TAAAGCATTG TTACCTAGGA 
CGGTGTATGT TTGATTTGAA TTTTTCTGCT TTTGATAGAA ATTCAGAAAG CAATGATTTA 
AATGGTTTAG ATGACTCATT CAAGTTTTGG AGGGCTCCAT CTAGGACAAG TCAGGATCGA 
GATCCAAGTT CTCTCTCCAC CTCAGAAACA ACGGTGGCAC CTTCATTGGG GAGTACTGAA 
TTTCAGCCAC TTGTGCAGTC AACAACACTT CTACCTGAAG CCTCCCATGA CCAGTTTGTG 
GCTCAAGGTC ACCAGGAAAG TACATCACCA CGGCCACCTC ATGATTCATC TCTTTCTGCT 
CCCCTGCCCA AACTGTGTGT TTTTGTTACT CCATCTCTTC TTTCAGCAAT GTGCAGCCTC 
TTGGACAACC TCTTGACGAT TGCTCCCAGA GACACTGCAA AGGCTTTTCG ACAAGCTCAT 
CTCATAGAAC TTCTCTGTAG CATTGCAGAT GCTACCCTCA TACAGACATG TGTCCAGGAA 
CTCAGAGCCC TGCTGCCTTC ATCACCTCCA GCTGAACACA CTCAGGCTCA GGTTTCCTTT 
CTCCTGGAAT ACCTATCCTC TTTGTCCAGG CTTCTGCAGT CATGTTTATT GGTGGAGCCT 
GACCTTGTGA TTCAGGATGA GCTTGTGAAA CCTCTTATCA CCAATATCAT TGGAATTCTC 
ACCATATGTA CCAAAGATGT ATTAGATAAA GAGTTAATAT CAGCTTTTTA TCACACATGG 
ACACATTTAT TTAATCTTCT GGCCATGCTC CTGAGGAAAG CTGGTGCCAT CACACTCCCG 
TTTGTTACCG TGGCCCTGGC CAAGCACTGG ACAGCGGCGA TTGATATGTT CTGCACATGT 
GCAGGCTTGT CTGCCACGTG TCCTGCCCTG TATACTGCCA GCTTGCAATT CCTTTCTGTT 
CTCTTGACCG AAGAAGCAAA AGGGCATCTC CAGGCTAAGA GCAAAACACA TTTATGCTGT 
AGTCCAACAG TGGCTTCACT TCTTGATGAC TCTCAGGAAA ATCAGAAATC TCTAGAACAA 
CTTAGTGATG TAATCCTTCA GTGCTATGAA GGGAAATCCT CCAAAGATAT CCTGAAAAGA 
GTAGCTGCAA ATGCATTGAT GTCACTGCTG GCTGTCAGTA GAAGAGCACA GAAACATGCT 
TTGAAAGCCA ATCTTATAGA CAATTGCATG GAGCAGATGA AACACATAAA TGCACAACTG 
AACCTAGATT CTCTGAGGCC TGGGAAAGCA GCATTGAAAA AAAAGGAGGA TGGTGTTATT 
AAAGAGTTAA GCATTGCCAT GCAGCTCCTA AGAAACTGTC TTTATCAAAA TGAGGAATGT 
AAAGAAGCAG CTCTTGAAGC TCACCTTGTC CCTGTCTTGC ACTCTCTCTG GCCTTGGATT 
TTGATGGATG ATTCATTGAT GCAAATTTCT CTGCAGCTCC TTTGTGTCTA TACTGCAAAT 
TTTCCAAATG GTTGCAGTTC TCTTTGTTGG TCAAGTTGTG GACAACACCC TGTTCAAGCT 
ACACATAGAG GAGCCGTGAG CAACTCTCTG ATGCTGTGTA TCCTAAAGTT GGCTTCCCAG 
ATGCCACTGG AGAACACCAC GGTTCAGCAG ATGGTTTTTA TGCTTCTTTC AAACCTGGCC 
TTGTCGCATG ACTGTAAAGG AGTAATTCAG AAGAGTAACT TCTTACAGAA CTTCCTCTCT 
CTAGCATTGC CAAAAGGAGG AAATAAACAT CTAAGTAATC TGACTATTCT TTGGTTGAAG 
TTACTCCTGA ATATATCATC TGGAGAAGAT GGGCAACAAA TGATTCTGAG GCTTGATGGC 
TGTCTAGACT TACTAACAGA GATGAGCAAA TACAAGCACA AGAGCAGCCC TTTATTGCCT 
CTTCTTATCT TTCATAATGT TTGCTTCAGT CCTGCAAATA AACCCAAGAT CCTGGCTAAT 
GAAAAAGTCA TTACTGTGCT TGCTGCCTGT CTGGAAAGTG AGAATCAAAA TGCTCAGAGG 
ATTGGAGCAG CTGCCCTTTG GGCTCTGATT TACAATTATC AGAAGGCAAA AACAGCTTTG 
AAAAGCCCAT CAGTAAAAAG AAGAGTGGAT GAAGCATACT CCTTAGCAAA GAAAACTTTC 
CCAAACTCAG AAGCAAACCC TCTAAATGCC TATTATTTGA AATGTCTTGA AAACCTCGTG 
CAGCTCCTTA ATTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_006722434.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722497.1 Homo sapiens rotatin (RTTN), transcript variant X1, mRNA.

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CloneID	OHu51153
Clone ID Related Accession (Same CDS sequence)	XM_006722434.3
Accession Version	XM_006722434.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722434.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722497.1
CDS	69..6752
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_006722434.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722434.3

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_006722435.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722498.1 Homo sapiens rotatin (RTTN), transcript variant X5, mRNA.

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CloneID	OHu51154
Clone ID Related Accession (Same CDS sequence)	XM_006722435.3
Accession Version	XM_006722435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGGCCA AGATAAATTG CAGAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722498.1
CDS	69..6248
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKAKINCRS

Target ORF information:

RefSeq Version	XM_006722435.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722435.3

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAAATT TTAGATGCAT TGAAGTTATC TACAGAGGAC 
ATCCTCACTC TGAAGATAAC ACACATGGCT AGTGGGCTGC AGGACTGCCT CCATTCCATT 
GTTCAGGCTG CAACCCACAG GGAAGTTAGG GCTGCTGTCA CCAGGATGAG TTTTTATCTT 
CTGAATGACA GATTGTCTTT AAAGGGTTGC CCTGGTCCAT GTGGGGTTAC CTTGAAGTCC 
TTGGCTTGGC ACACCGCACT AAACAGGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGGCCA AGATAAATTG CAGAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525902.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524204.1 Homo sapiens rotatin (RTTN), transcript variant X2, mRNA.

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CloneID	OHu66630
Clone ID Related Accession (Same CDS sequence)	XM_011525902.2
Accession Version	XM_011525902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524204.1
CDS	69..6512
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_011525902.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525902.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTA CCATCTACCT GTTCATGTAA TTGGACACCA TGCTGTGAGT 
CCTTACTCCA TAGTTTTGCC CTTATCTGCT GATTGTTTGG CCTTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGTCATGG TATCATGGGA GTGATAATCT GTTGAAGCAA 
ATGAACTCTG AAACTAAAAC GCAAGAGTTT TTACAGGTGC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAAT TAATAAAGGA ACAAAGAAAA 
AATTCTTCAC TAGAACTTCT AAACTGGATT CTCGAATTAC TTCTTAGACA TAGTGCAAAC 
CCACTGTTAG ACCTCTTGGT TCTGACAGAG TCACAGGCAC GAGAAGAAAC AGATGATATC 
CGGACTGCTG TCAGGCAACA ACTTCAGAAA GAACTGATTG CTCTTTTTGA TACCTTGCTG 
CTCAATTTCA TGGAAGTTAC TGACAGGAAA TGCTCGGAAC TTCTTTACGT TTTTCAAACG 
CAGCTGGCTC TGAAACTGCT CCAGTGTCTG AAAGTGACGG ATGCGCCTCA TTTCTATGGC 
CTGCCGTCCC TTGAGCGGAC CTTACGAGGG ATGGCTAACC TCACTGCGTT TCCGGGATGG 
AGCTCACACT CTCCTCTCAC AAAGCCTCTA GATATCTGTG TGAAGTACTT GTCAGGTCTC 
CTTGAGGTCA TTACTTCTTT TTATGTGGAG CGTGGAGGAA ATGCTATGTC CTTCATGGGA 
AAAGGTGTTA CAAAGAGCAC AATTCTTTGC TTGCTTCACT TATCCCATGA GATGATGGCC 
CAGGCTGGGA GCTTGGAGTG GATGTCACTT TGGTTCTTGC CTTTGGGTAG TCATAGTGAA 
GAACATATTC CTACTCAACA AGGATTGGCT TGGTTGATTC CATTATGGGT TGATCGGGAC 
CCAGAGGTGA GATTCACTTC ACTGGGATTA GGATCAGCAC TGACCACCCT TGAAACGGGC 
TGTGTGGCCT TAGCAAACAG TTGTCAGAAC ATTTCCGGTG GGCTCTGGGG AACTGTGGTG 
AACATTCTTC TGGACCAGTC AGAATGTAGT ATGGTGCGCC GGGAGGCGGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTACA GAAATTATAA AGGATTATAC TTGGCAGGGT 
CCCTGTGTTC ATGATGAGGA CTCTGGCCTA TCGCTCATTG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAGCA TTGTTACCTA 
GGACGGTGTA TGTTTGATTT GAATTTTTCT GCTTTTGATA GAAATTCAGA AAGCAATGAT 
TTAAATGGTT TAGATGACTC ATTCAAGTTT TGGAGGGCTC CATCTAGGAC AAGTCAGGAT 
CGAGATCCAA GTTCTCTCTC CACCTCAGAA ACAACGGTGG CACCTTCATT GGGGAGTACT 
GAATTTCAGC CACTTGTGCA GTCAACAACA CTTCTACCTG AAGCCTCCCA TGACCAGTTT 
GTGGCTCAAG GTCACCAGGA AAGTACATCA CCACGGCCAC CTCATGATTC ATCTCTTTCT 
GCTCCCCTGC CCAAACTGTG TGTTTTTGTT ACTCCATCTC TTCTTTCAGC AATGTGCAGC 
CTCTTGGACA ACCTCTTGAC GATTGCTCCC AGAGACACTG CAAAGGCTTT TCGACAAGCT 
CATCTCATAG AACTTCTCTG TAGCATTGCA GATGCTACCC TCATACAGAC ATGTGTCCAG 
GAACTCAGAG CCCTGCTGCC TTCATCACCT CCAGCTGAAC ACACTCAGGC TCAGGTTTCC 
TTTCTCCTGG AATACCTATC CTCTTTGTCC AGGCTTCTGC AGTCATGTTT ATTGGTGGAG 
CCTGACCTTG TGATTCAGGA TGAGCTTGTG AAACCTCTTA TCACCAATAT CATTGGAATT 
CTCACCATAT GTACCAAAGA TGTATTAGAT AAAGAGTTAA TATCAGCTTT TTATCACACA 
TGGACACATT TATTTAATCT TCTGGCCATG CTCCTGAGGA AAGCTGGTGC CATCACACTC 
CCGTTTGTTA CCGTGGCCCT GGCCAAGCAC TGGACAGCGG CGATTGATAT GTTCTGCACA 
TGTGCAGGCT TGTCTGCCAC GTGTCCTGCC CTGTATACTG CCAGCTTGCA ATTCCTTTCT 
GTTCTCTTGA CCGAAGAAGC AAAAGGGCAT CTCCAGGCTA AGAGCAAAAC ACATTTATGC 
TGTAGTCCAA CAGTGGCTTC ACTTCTTGAT GACTCTCAGG AAAATCAGAA ATCTCTAGAA 
CAACTTAGTG ATGTAATCCT TCAGTGCTAT GAAGGGAAAT CCTCCAAAGA TATCCTGAAA 
AGAGTAGCTG CAAATGCATT GATGTCACTG CTGGCTGTCA GTAGAAGAGC ACAGAAACAT 
GCTTTGAAAG CCAATCTTAT AGACAATTGC ATGGAGCAGA TGAAACACAT AAATGCACAA 
CTGAACCTAG ATTCTCTGAG GCCTGGGAAA GCAGCATTGA AAAAAAAGGA GGATGGTGTT 
ATTAAAGAGT TAAGCATTGC CATGCAGCTC CTAAGAAACT GTCTTTATCA AAATGAGGAA 
TGTAAAGAAG CAGCTCTTGA AGCTCACCTT GTCCCTGTCT TGCACTCTCT CTGGCCTTGG 
ATTTTGATGG ATGATTCATT GATGCAAATT TCTCTGCAGC TCCTTTGTGT CTATACTGCA 
AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT GTGGACAACA CCCTGTTCAA 
GCTACACATA GAGGAGCCGT GAGCAACTCT CTGATGCTGT GTATCCTAAA GTTGGCTTCC 
CAGATGCCAC TGGAGAACAC CACGGTTCAG CAGATGGTTT TTATGCTTCT TTCAAACCTG 
GCCTTGTCGC ATGACTGTAA AGGAGTAATT CAGAAGAGTA ACTTCTTACA GAACTTCCTC 
TCTCTAGCAT TGCCAAAAGG AGGAAATAAA CATCTAAGTA ATCTGACTAT TCTTTGGTTG 
AAGTTACTCC TGAATATATC ATCTGGAGAA GATGGGCAAC AAATGATTCT GAGGCTTGAT 
GGCTGTCTAG ACTTACTAAC AGAGATGAGC AAATACAAGC ACAAGAGCAG CCCTTTATTG 
CCTCTTCTTA TCTTTCATAA TGTTTGCTTC AGTCCTGCAA ATAAACCCAA GATCCTGGCT 
AATGAAAAAG TCATTACTGT GCTTGCTGCC TGTCTGGAAA GTGAGAATCA AAATGCTCAG 
AGGATTGGAG CAGCTGCCCT TTGGGCTCTG ATTTACAATT ATCAGAAGGC AAAAACAGCT 
TTGAAAAGCC CATCAGTAAA AAGAAGAGTG GATGAAGCAT ACTCCTTAGC AAAGAAAACT 
TTCCCAAACT CAGAAGCAAA CCCTCTAAAT GCCTATTATT TGAAATGTCT TGAAAACCTC 
GTGCAGCTCC TTAATTCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RTTN ( XM_011525903.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524205.1 Homo sapiens rotatin (RTTN), transcript variant X4, mRNA.

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CloneID	OHu66631
Clone ID Related Accession (Same CDS sequence)	XM_011525903.2
Accession Version	XM_011525903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rotatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTT TTTACAGGTG CTTCCTGCTT GCACTGAAGA TGAGAAACTG 
CTAATAGATA TCATACATTT TTTAAATAAA TTAATAAAGG AACAAAGAAA AAATTCTTCA 
CTAGAACTTC TAAACTGGAT TCTCGAATTA CTTCTTAGAC ATAGTGCAAA CCCACTGTTA 
GACCTCTTGG TTCTGACAGA GTCACAGGCA CGAGAAGAAA CAGATGATAT CCGGACTGCT 
GTCAGGCAAC AACTTCAGAA AGAACTGATT GCTCTTTTTG ATACCTTGCT GCTCAATTTC 
ATGGAAGTTA CTGACAGGAA ATGCTCGGAA CTTCTTTACG TTTTTCAAAC GCAGCTGGCT 
CTGAAACTGC TCCAGTGTCT GAAAGTGACG GATGCGCCTC ATTTCTATGG CCTGCCGTCC 
CTTGAGCGGA CCTTACGAGG GATGGCTAAC CTCACTGCGT TTCCGGGATG GAGCTCACAC 
TCTCCTCTCA CAAAGCCTCT AGATATCTGT GTGAAGTACT TGTCAGGTCT CCTTGAGGTC 
ATTACTTCTT TTTATGTGGA GCGTGGAGGA AATGCTATGT CCTTCATGGG AAAAGGTGTT 
ACAAAGAGCA CAATTCTTTG CTTGCTTCAC TTATCCCATG AGATGATGGC CCAGGCTGGG 
AGCTTGGAGT GGATGTCACT TTGGTTCTTG CCTTTGGGTA GTCATAGTGA AGAACATATT 
CCTACTCAAC AAGGATTGGC TTGGTTGATT CCATTATGGG TTGATCGGGA CCCAGAGGTG 
AGATTCACTT CACTGGGATT AGGATCAGCA CTGACCACCC TTGAAACGGG CTGTGTGGCC 
TTAGCAAACA GTTGTCAGAA CATTTCCGGT GGGCTCTGGG GAACTGTGGT GAACATTCTT 
CTGGACCAGT CAGAATGTAG TATGGTGCGC CGGGAGGCGG CATTTATTCT TCAGAATCTC 
CTTGTAATTC CAATGCCTAC AGAAATTATA AAGGATTATA CTTGGCAGGG TCCCTGTGTT 
CATGATGAGG ACTCTGGCCT ATCGCTCATT GGAAAACCTG CCCTTCAGGC TCTTTTATAT 
CACTGCCATT TTTATGAACA TTTGAATCAG ATGGTAAAGC ATTGTTACCT AGGACGGTGT 
ATGTTTGATT TGAATTTTTC TGCTTTTGAT AGAAATTCAG AAAGCAATGA TTTAAATGGT 
TTAGATGACT CATTCAAGTT TTGGAGGGCT CCATCTAGGA CAAGTCAGGA TCGAGATCCA 
AGTTCTCTCT CCACCTCAGA AACAACGGTG GCACCTTCAT TGGGGAGTAC TGAATTTCAG 
CCACTTGTGC AGTCAACAAC ACTTCTACCT GAAGCCTCCC ATGACCAGTT TGTGGCTCAA 
GGTCACCAGG AAAGTACATC ACCACGGCCA CCTCATGATT CATCTCTTTC TGCTCCCCTG 
CCCAAACTGT GTGTTTTTGT TACTCCATCT CTTCTTTCAG CAATGTGCAG CCTCTTGGAC 
AACCTCTTGA CGATTGCTCC CAGAGACACT GCAAAGGCTT TTCGACAAGC TCATCTCATA 
GAACTTCTCT GTAGCATTGC AGATGCTACC CTCATACAGA CATGTGTCCA GGAACTCAGA 
GCCCTGCTGC CTTCATCACC TCCAGCTGAA CACACTCAGG CTCAGGTTTC CTTTCTCCTG 
GAATACCTAT CCTCTTTGTC CAGGCTTCTG CAGTCATGTT TATTGGTGGA GCCTGACCTT 
GTGATTCAGG ATGAGCTTGT GAAACCTCTT ATCACCAATA TCATTGGAAT TCTCACCATA 
TGTACCAAAG ATGTATTAGA TAAAGAGTTA ATATCAGCTT TTTATCACAC ATGGACACAT 
TTATTTAATC TTCTGGCCAT GCTCCTGAGG AAAGCTGGTG CCATCACACT CCCGTTTGTT 
ACCGTGGCCC TGGCCAAGCA CTGGACAGCG GCGATTGATA TGTTCTGCAC ATGTGCAGGC 
TTGTCTGCCA CGTGTCCTGC CCTGTATACT GCCAGCTTGC AATTCCTTTC TGTTCTCTTG 
ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT AAGAGCAAAA CACATTTATG CTGTAGTCCA 
ACAGTGGCTT CACTTCTTGA TGACTCTCAG GAAAATCAGA AATCTCTAGA ACAACTTAGT 
GATGTAATCC TTCAGTGCTA TGAAGGGAAA TCCTCCAAAG ATATCCTGAA AAGAGTAGCT 
GCAAATGCAT TGATGTCACT GCTGGCTGTC AGTAGAAGAG CACAGAAACA TGCTTTGAAA 
GCCAATCTTA TAGACAATTG CATGGAGCAG ATGAAACACA TAAATGCACA ACTGAACCTA 
GATTCTCTGA GGCCTGGGAA AGCAGCATTG AAAAAAAAGG AGGATGGTGT TATTAAAGAG 
TTAAGCATTG CCATGCAGCT CCTAAGAAAC TGTCTTTATC AAAATGAGGA ATGTAAAGAA 
GCAGCTCTTG AAGCTCACCT TGTCCCTGTC TTGCACTCTC TCTGGCCTTG GATTTTGATG 
GATGATTCAT TGATGCAAAT TTCTCTGCAG CTCCTTTGTG TCTATACTGC AAATTTTCCA 
AATGGTTGCA GTTCTCTTTG TTGGTCAAGT TGTGGACAAC ACCCTGTTCA AGCTACACAT 
AGAGGAGCCG TGAGCAACTC TCTGATGCTG TGTATCCTAA AGTTGGCTTC CCAGATGCCA 
CTGGAGAACA CCACGGTTCA GCAGATGGTT TTTATGCTTC TTTCAAACCT GGCCTTGTCG 
CATGACTGTA AAGGAGTAAT TCAGAAGAGT AACTTCTTAC AGAACTTCCT CTCTCTAGCA 
TTGCCAAAAG GAGGAAATAA ACATCTAAGT AATCTGACTA TTCTTTGGTT GAAGTTACTC 
CTGAATATAT CATCTGGAGA AGATGGGCAA CAAATGATTC TGAGGCTTGA TGGCTGTCTA 
GACTTACTAA CAGAGATGAG CAAATACAAG CACAAGAGCA GCCCTTTATT GCCTCTTCTT 
ATCTTTCATA ATGTTTGCTT CAGTCCTGCA AATAAACCCA AGATCCTGGC TAATGAAAAA 
GTCATTACTG TGCTTGCTGC CTGTCTGGAA AGTGAGAATC AAAATGCTCA GAGGATTGGA 
GCAGCTGCCC TTTGGGCTCT GATTTACAAT TATCAGAAGG CAAAAACAGC TTTGAAAAGC 
CCATCAGTAA AAAGAAGAGT GGATGAAGCA TACTCCTTAG CAAAGAAAAC TTTCCCAAAC 
TCAGAAGCAA ACCCTCTAAA TGCCTATTAT TTGAAATGTC TTGAAAACCT CGTGCAGCTC 
CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524205.1
CDS	69..6323
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQAEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPRPVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAASSSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVPLLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETMCYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEQVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDARVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS

Target ORF information:

RefSeq Version	XM_011525903.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rotatin (RTTN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525903.2

ORF Insert Sequence:

ATGGTCCTGG CAGGGCTCAT CAGGAAACTC GGTCATCAGC TGGCCGAGAT CAGGGAGCGC 
GCTCTCAAGA GTATTCTCTG CAAGATTGAG CACAACTTAA TCTGCTACGC TGATCTCATT 
CAGGAGAGGC AACTTTTTCT TCATTTGCTG GAATGGTTCA ATTTCCCGTC CGTTCCGATG 
AAGGAAGAGG TTCTGAACCT GTTAAGCAGA TTGGTTAAGT ATCCCCCAGC AGTCCAACAT 
TTGGTTGACG TTGGTGCAGT AGAGTTCTTA TCTAAGCTTC GGTCTAATGT GGAGCCAAAT 
CTGCAGGCTG AAATTGATGG CATTCTGGAT GGACTTTTTC TTCTTCCTTC GGAAGTTCCT 
GCACTATCTT CTGCCTCATA CCAAACCAAT CAAACTGAAT TGTCAAAAAA CCCTGAAATC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT GCCGCCACGA 
CCAGTGGTAA ATCAGACTGT GAAGTGCTTG AAGTTTTCTA CATTTCCTTG GCTACCCCTG 
ACCACCACAG ACAGACATGT CCTCTCCTCT AATGAAAGCT CTTTAAGAAG TAGTAACCAC 
ACTTTAATCT GGAACACCTG TGAACTATTG AAGGATGTTA TCATGCAAGA TTTTCCTGCT 
GAGATTTTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC 
TTTGGAGATG GAAAGCATCG CCTGGCATTA CAGTCGGTGT CCTGCCTGCA GCAGCTGTGC 
ATGTATTTAA GAAACAGACT TAACTTTCAC CGAGATCCAG GTTTTTTCTC CAATAAACAC 
GACACAGTTT CCCAAAATTC TTCTTTGTCT TATTGTCATG AAGCAAGAGG TACTCATCAT 
TCCCAGAATC CTTCCCCAGG AAGCAGCAGC CCTCGGCCTT CTGTGGTTGG GCGCACAGGC 
CAGCGACCCA GAGGAGATGG CCAGGACTGG GATGCAGCGT CCTCTAGTGG AAGTAGTAGT 
CATGCTCATG TAAACTCCAG GATATCCGTT CATTCACCTT TGGATATGGG ACACATAGAT 
CTGCCAGAGC TGGAAACTGA AGACACATTG GAACTACAAT TCCAGCAGCT CAGTCTTCCC 
CAGTTTTGTG TCTCCATTCT GGAATCAGCT GTTCCTCTCT TAAGAACAGG TAGCAGACAA 
GTGATAATAA GAGTTCTGGA ATTGCTTACA GAGGATATGA CACTTATTGG TGAAGCAATT 
TCAACAGATA TCTGGGATGA CAGCAGCCTT TTTGGTATAG ATATGAAGGA GAAACTACTC 
CTGGTGTTGG GAGCCCTTGG AGAAACCATG TGCTACCATA AAAGTAGTAT CAGTTTGGAG 
CAGCCAGAGG TGATGCTTGT GCACCACAGA ATGGCCTTTA TCAGCATTTC CCTGTTTGCA 
GTTCGACTGC TACAAACGCT TCTCCCTGTT GAAAAGGCAA GCGAGTTTTT ATCAGAGCCT 
ATGTCAACAG CATTATTTCT CCTTTCTTTG GACATGCCTA TTTCTTTGGA ATATCCAAAT 
ATTCATGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT 
AAACGAACTG CAGAGGCCGT TTATTCAATT GAATGCACCT GTAACTTCCT GTCTGATATT 
GGGAAAGAGG GAGAGAAGAA TCTCTTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTTCCCGCTA ATAAAGGAAA TCATCAGCAT TTGTTCAAAG 
ATCTGGAAAT CTGCTCAGGC CAGTCCATTA CTACAAGGAG AAAGTCAGAA GGTGCTTCTC 
CATATGTTGT CTCACCCATT GCCACGAGTG AAAGCTGAAA CTTACCACTG CTGTCTGGAA 
ATCACGAAGG AATGTTTAGG TGTCCATAAT GTCACTAAAC CCGTGTCTTC ACTTTGCAAT 
GGAATTCATT TTCTACTTCA TCCAAAAGTT CTGTATGAAA TCTCTGTATT TGGCATTCAA 
GAGCCCGAAA GTGAGCAGGT GAACACTGCT GCCAAGGCCA TTCTGTTATA CCTGCTTCAG 
GGACGATTGA TGATGACAGC ATTGACCTGG AACAAGTTTA TTGAATCTCT CTGTCCTGTC 
ATCCCAATAC TACAGGGCTA TGCCGACACA GAAGATCCTC TGGGTAACTG CATTCTCCTT 
CTAAGCAAAG CAAGTTCTGA CACAGAAGAG ATGCTTCCGT GTACTACCCG ATTAAAGTCC 
ATGCTGCGAC TTTTGCTAGT GAAAAAGCCA TCGGTCCGTT CGCTAGCATT AAAGCTTTTA 
GCATTCCATC TTACCAGTGA AGAAGGGGCT GATACAAAGC GTCCTCTAAT AGACGCCAGA 
GTTCTCTCCA GAGTTACTGA TTTATTCATT GGGAAGAAAC CTATTGAACT CAGACTGGAT 
GACAGAAGAG AGCTGGTTAT TAAGTTGGAG ACTGTTGAAA AGGTATATGA AATCTTCACC 
TCAGATGATG TTGATCTCGT TTTGAGAAAG TCAGCTGCTG AACAGTTAGC TGTGATTATG 
CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTATGCT TAATTGACAA AATAATTGAG 
TATTTAAATG AATGTGTGAG TCAAGATGGC AAGGTTGTAG AATGTTTGGT ACAACCATGC 
CTCACACTCT TGAGGAAGGT TTTATGTGGT GATCCAGTCA TGCGTGTTTC GCTCTCGCAA 
CAGTCTTCTC TTTTGACCGT GTTATTCAGA GTTTCCTTAA TATTTCATGA AGATTGTAGT 
GTCGTGACTG AAGTTGGAGC ACTATTTTGT CTTCTATTAT TTGATGAAGT ATCGAGAATG 
GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTTGC CATCGGTCTT CAGTTTGCCT 
GTTTCCGTTT TTAGAAGGTT TTTACAGGTG CTTCCTGCTT GCACTGAAGA TGAGAAACTG 
CTAATAGATA TCATACATTT TTTAAATAAA TTAATAAAGG AACAAAGAAA AAATTCTTCA 
CTAGAACTTC TAAACTGGAT TCTCGAATTA CTTCTTAGAC ATAGTGCAAA CCCACTGTTA 
GACCTCTTGG TTCTGACAGA GTCACAGGCA CGAGAAGAAA CAGATGATAT CCGGACTGCT 
GTCAGGCAAC AACTTCAGAA AGAACTGATT GCTCTTTTTG ATACCTTGCT GCTCAATTTC 
ATGGAAGTTA CTGACAGGAA ATGCTCGGAA CTTCTTTACG TTTTTCAAAC GCAGCTGGCT 
CTGAAACTGC TCCAGTGTCT GAAAGTGACG GATGCGCCTC ATTTCTATGG CCTGCCGTCC 
CTTGAGCGGA CCTTACGAGG GATGGCTAAC CTCACTGCGT TTCCGGGATG GAGCTCACAC 
TCTCCTCTCA CAAAGCCTCT AGATATCTGT GTGAAGTACT TGTCAGGTCT CCTTGAGGTC 
ATTACTTCTT TTTATGTGGA GCGTGGAGGA AATGCTATGT CCTTCATGGG AAAAGGTGTT 
ACAAAGAGCA CAATTCTTTG CTTGCTTCAC TTATCCCATG AGATGATGGC CCAGGCTGGG 
AGCTTGGAGT GGATGTCACT TTGGTTCTTG CCTTTGGGTA GTCATAGTGA AGAACATATT 
CCTACTCAAC AAGGATTGGC TTGGTTGATT CCATTATGGG TTGATCGGGA CCCAGAGGTG 
AGATTCACTT CACTGGGATT AGGATCAGCA CTGACCACCC TTGAAACGGG CTGTGTGGCC 
TTAGCAAACA GTTGTCAGAA CATTTCCGGT GGGCTCTGGG GAACTGTGGT GAACATTCTT 
CTGGACCAGT CAGAATGTAG TATGGTGCGC CGGGAGGCGG CATTTATTCT TCAGAATCTC 
CTTGTAATTC CAATGCCTAC AGAAATTATA AAGGATTATA CTTGGCAGGG TCCCTGTGTT 
CATGATGAGG ACTCTGGCCT ATCGCTCATT GGAAAACCTG CCCTTCAGGC TCTTTTATAT 
CACTGCCATT TTTATGAACA TTTGAATCAG ATGGTAAAGC ATTGTTACCT AGGACGGTGT 
ATGTTTGATT TGAATTTTTC TGCTTTTGAT AGAAATTCAG AAAGCAATGA TTTAAATGGT 
TTAGATGACT CATTCAAGTT TTGGAGGGCT CCATCTAGGA CAAGTCAGGA TCGAGATCCA 
AGTTCTCTCT CCACCTCAGA AACAACGGTG GCACCTTCAT TGGGGAGTAC TGAATTTCAG 
CCACTTGTGC AGTCAACAAC ACTTCTACCT GAAGCCTCCC ATGACCAGTT TGTGGCTCAA 
GGTCACCAGG AAAGTACATC ACCACGGCCA CCTCATGATT CATCTCTTTC TGCTCCCCTG 
CCCAAACTGT GTGTTTTTGT TACTCCATCT CTTCTTTCAG CAATGTGCAG CCTCTTGGAC 
AACCTCTTGA CGATTGCTCC CAGAGACACT GCAAAGGCTT TTCGACAAGC TCATCTCATA 
GAACTTCTCT GTAGCATTGC AGATGCTACC CTCATACAGA CATGTGTCCA GGAACTCAGA 
GCCCTGCTGC CTTCATCACC TCCAGCTGAA CACACTCAGG CTCAGGTTTC CTTTCTCCTG 
GAATACCTAT CCTCTTTGTC CAGGCTTCTG CAGTCATGTT TATTGGTGGA GCCTGACCTT 
GTGATTCAGG ATGAGCTTGT GAAACCTCTT ATCACCAATA TCATTGGAAT TCTCACCATA 
TGTACCAAAG ATGTATTAGA TAAAGAGTTA ATATCAGCTT TTTATCACAC ATGGACACAT 
TTATTTAATC TTCTGGCCAT GCTCCTGAGG AAAGCTGGTG CCATCACACT CCCGTTTGTT 
ACCGTGGCCC TGGCCAAGCA CTGGACAGCG GCGATTGATA TGTTCTGCAC ATGTGCAGGC 
TTGTCTGCCA CGTGTCCTGC CCTGTATACT GCCAGCTTGC AATTCCTTTC TGTTCTCTTG 
ACCGAAGAAG CAAAAGGGCA TCTCCAGGCT AAGAGCAAAA CACATTTATG CTGTAGTCCA 
ACAGTGGCTT CACTTCTTGA TGACTCTCAG GAAAATCAGA AATCTCTAGA ACAACTTAGT 
GATGTAATCC TTCAGTGCTA TGAAGGGAAA TCCTCCAAAG ATATCCTGAA AAGAGTAGCT 
GCAAATGCAT TGATGTCACT GCTGGCTGTC AGTAGAAGAG CACAGAAACA TGCTTTGAAA 
GCCAATCTTA TAGACAATTG CATGGAGCAG ATGAAACACA TAAATGCACA ACTGAACCTA 
GATTCTCTGA GGCCTGGGAA AGCAGCATTG AAAAAAAAGG AGGATGGTGT TATTAAAGAG 
TTAAGCATTG CCATGCAGCT CCTAAGAAAC TGTCTTTATC AAAATGAGGA ATGTAAAGAA 
GCAGCTCTTG AAGCTCACCT TGTCCCTGTC TTGCACTCTC TCTGGCCTTG GATTTTGATG 
GATGATTCAT TGATGCAAAT TTCTCTGCAG CTCCTTTGTG TCTATACTGC AAATTTTCCA 
AATGGTTGCA GTTCTCTTTG TTGGTCAAGT TGTGGACAAC ACCCTGTTCA AGCTACACAT 
AGAGGAGCCG TGAGCAACTC TCTGATGCTG TGTATCCTAA AGTTGGCTTC CCAGATGCCA 
CTGGAGAACA CCACGGTTCA GCAGATGGTT TTTATGCTTC TTTCAAACCT GGCCTTGTCG 
CATGACTGTA AAGGAGTAAT TCAGAAGAGT AACTTCTTAC AGAACTTCCT CTCTCTAGCA 
TTGCCAAAAG GAGGAAATAA ACATCTAAGT AATCTGACTA TTCTTTGGTT GAAGTTACTC 
CTGAATATAT CATCTGGAGA AGATGGGCAA CAAATGATTC TGAGGCTTGA TGGCTGTCTA 
GACTTACTAA CAGAGATGAG CAAATACAAG CACAAGAGCA GCCCTTTATT GCCTCTTCTT 
ATCTTTCATA ATGTTTGCTT CAGTCCTGCA AATAAACCCA AGATCCTGGC TAATGAAAAA 
GTCATTACTG TGCTTGCTGC CTGTCTGGAA AGTGAGAATC AAAATGCTCA GAGGATTGGA 
GCAGCTGCCC TTTGGGCTCT GATTTACAAT TATCAGAAGG CAAAAACAGC TTTGAAAAGC 
CCATCAGTAA AAAGAAGAGT GGATGAAGCA TACTCCTTAG CAAAGAAAAC TTTCCCAAAC 
TCAGAAGCAA ACCCTCTAAA TGCCTATTAT TTGAAATGTC TTGAAAACCT CGTGCAGCTC 
CTTAATTCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RTTN
Entrez Gene ID	25914
Full Name	rotatin
Synonyms	MSSP
General protein information	Preferred Names rotatin Names rotatin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q22.2
Summary	This gene encodes a large protein whose specific function is unknown. Absence of the orthologous protein in mouse results in embryonic lethality with deficient axial rotation, abnormal differentiation of the neural tube, and randomized looping of the heart tube during development. In human, mutations in this gene are associated with polymicrogyria with seizures. In human fibroblasts this protein localizes at the ciliary basal bodies. Given the intracellular localization of this protein and the phenotypic effects of mutations, this gene is suspected of playing a role in the maintenance of normal ciliary structure which in turn effects the developmental process of left-right organ specification, axial rotation, and perhaps notochord development. [provided by RefSeq, Jan 2013].
Disorder MIM:	610436
Disorder Html:	Microcephaly, short stature, and polymicrogyria with seizures, 614833 (3)

	RTTN	XP_001095442.1
Canis lupus familiaris (dog)	RTTN	XP_541060.2
Bos taurus (cattle)	RTTN	NP_001193136.1
Mus musculus (house mouse)	Rttn	NP_780751.2
Homo sapiens (human)	RTTN	NP_775901.3
Danio rerio (zebrafish)	rttn	XP_001920443.3
Xenopus tropicalis (tropical clawed frog)	rttn	XP_002939666.2
Gallus gallus (chicken)	RTTN	NP_001243472.1
Pan troglodytes (chimpanzee)	RTTN	XP_512171.3
Rattus norvegicus (Norway rat)	Rttn	NP_001163907.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RTTN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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