HIST1H2BA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIST1H2BA
Entrez Gene ID	255626
Full Name	histone cluster 1 H2B family member a
Synonyms	H2BFU,STBP,TH2B,TSH2B,TSH2B.1,bA317E16.3,hTSH2B
General protein information	Preferred Names histone cluster 1 H2B family member a Names histone H2B type 1-A H2B histone family, member U, (testis-specific) histone 1, H2ba histone H2B, testis histone cluster 1, H2ba testis-specific histone H2B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015].
Disorder MIM:	609904

mRNA and Protein(s)

mRNA	Protein	Name
NM_170610.3	NP_733759.1	histone H2B type 1-A
NM_170610.2	NP_733759.1	histone H2B type 1-A

Pathways from BioSystems

Homologies

	HIST1H2BA	XP_001086992.1
Rattus norvegicus (Norway rat)	Hist1h2ba	NP_072169.1
Bos taurus (cattle)	HIST1H2BA	XP_005223866.1
Mus musculus (house mouse)	Hist1h2ba	NP_783594.1
Homo sapiens (human)	HIST1H2BA	NP_733759.1
Pan troglodytes (chimpanzee)	HIST1H2BA	XP_527247.1
Canis lupus familiaris (dog)	HIST1H2BA	XP_545374.2
Canis lupus familiaris (dog)	LOC488253	XP_005640164.1

Gene Ontology
Bibliography

Related articles in PubMed

The inhibition of lactate dehydrogenase A hinders the transcription of histone 2B gene independently from the block of aerobic glycolysis.
Brighenti E, Carnicelli D, Brigotti M, Fiume L
Biochemical and biophysical research communications485(4)742-745(2017 Apr)

Structural analyses of the nucleosome complexes with human testis-specific histone variants, hTh2a and hTh2b.
Padavattan S, Thiruselvam V, Shinagawa T, Hasegawa K, Kumasaka T, Ishii S, Kumarevel T
Biophysical chemistry22141-48(2017 Feb)

Nick Your DNA, Mark Your Chromatin.
Nardozza AP, Ladurner AG
Molecular cell64(1)7-9(2016 Oct)

Two Histone Variants TH2A and TH2B Enhance Human Induced Pluripotent Stem Cell Generation.
Huynh LM, Shinagawa T, Ishii S
Stem cells and development25(3)251-8(2016 Feb)

Hypoacetylation, hypomethylation, and dephosphorylation of H2B histones and excessive histone deacetylase activity in DU-145 prostate cancer cells.
Cang S, Xu X, Ma Y, Liu D, Chiao JW
Journal of hematology & oncology93(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that lactate dehydrogenase A (LDHA) is involved in the transcription of histone 2B gene.
Title: The inhibition of lactate dehydrogenase A hinders the transcription of histone 2B gene independently from the block of aerobic glycolysis.

In a process of single-strand DNA repair, PARP3 mono-ADP-ribosylates nucleosomal histone H2B.
Title: Nick Your DNA, Mark Your Chromatin.

Data show that histone H2B of prostate cancer cell line DU-145 have hypoacetylation, hypomethylation, and dephosphorylation, suggesting there was an excessive histone deacetylase activity in the cells.
Title: Hypoacetylation, hypomethylation, and dephosphorylation of H2B histones and excessive histone deacetylase activity in DU-145 prostate cancer cells.

Reconstituted TSH2B containing octamers are able to form nucleosome core particles which are structurally and dynamically indistinguishable from those reconstituted with octamers consisting of only native histones.
Title: Characterization of nucleosomes consisting of the human testis/sperm-specific histone H2B variant (hTSH2B).

The following HIST1H2BA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H2BA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24747	NM_170610.3 Latest version!	Homo sapiens H2B clustered histone 1 (H2BC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu24747	NM_170610.2	Homo sapiens histone cluster 1 H2B family member a (HIST1H2BA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIST1H2BA ( NM_170610.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_733759.1 Homo sapiens histone cluster 1 H2B family member a (HIST1H2BA), mRNA.

Price & Availability↑

CloneID	OHu24747
Clone ID Related Accession (Same CDS sequence)	NM_170610.3 , NM_170610.2
Accession Version	NM_170610.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	histone H2B type 1-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF397301.1. On Feb 25, 2003 this sequence version replaced NM_170610.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAGG TGTCATCTAA AGGTGCTACC ATTTCCAAGA AGGGCTTTAA GAAAGCTGTC 
GTTAAGACCC AGAAAAAGGA AGGCAAAAAG CGCAAGAGGA CCCGTAAGGA GAGTTATTCT 
ATTTACATCT ACAAAGTGCT AAAGCAGGTC CATCCGGACA CTGGCATCTC TTCGAAAGCT 
ATGAGCATTA TGAATTCCTT CGTCACTGAT ATCTTTGAGC GTATAGCGAG CGAGGCATCA 
CGTTTGGCTC ACTACAGCAA GCGCTCCACC ATTTCTTCCA GAGAGATTCA GACAGCAGTG 
CGCTTGCTAC TGCCGGGAGA GCTGGCTAAA CATGCTGTGT CTGAGGGCAC CAAGGCTGTC 
ACTAAGTACA CCAGCTCCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733759.1
CDS	1..384
Translation	MPEVSSKGATISKKGFKKAVVKTQKKEGKKRKRTRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYSKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

Target ORF information:

RefSeq Version	NM_170610.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 1 H2B family member a (HIST1H2BA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170610.2

ORF Insert Sequence:

ATGCCGGAGG TGTCATCTAA AGGTGCTACC ATTTCCAAGA AGGGCTTTAA GAAAGCTGTC 
GTTAAGACCC AGAAAAAGGA AGGCAAAAAG CGCAAGAGGA CCCGTAAGGA GAGTTATTCT 
ATTTACATCT ACAAAGTGCT AAAGCAGGTC CATCCGGACA CTGGCATCTC TTCGAAAGCT 
ATGAGCATTA TGAATTCCTT CGTCACTGAT ATCTTTGAGC GTATAGCGAG CGAGGCATCA 
CGTTTGGCTC ACTACAGCAA GCGCTCCACC ATTTCTTCCA GAGAGATTCA GACAGCAGTG 
CGCTTGCTAC TGCCGGGAGA GCTGGCTAAA CATGCTGTGT CTGAGGGCAC CAAGGCTGTC 
ACTAAGTACA CCAGCTCCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIST1H2BA ( NM_170610.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_733759.1 Homo sapiens H2B clustered histone 1 (H2BC1), mRNA.

Price & Availability↑

CloneID	OHu24747
Clone ID Related Accession (Same CDS sequence)	NM_170610.3 , NM_170610.2
Accession Version	NM_170610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	histone H2B type 1-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512384.9. On Dec 3, 2019 this sequence version replaced NM_170610.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: DB084768.1, AK312055.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAGG TGTCATCTAA AGGTGCTACC ATTTCCAAGA AGGGCTTTAA GAAAGCTGTC 
GTTAAGACCC AGAAAAAGGA AGGCAAAAAG CGCAAGAGGA CCCGTAAGGA GAGTTATTCT 
ATTTACATCT ACAAAGTGCT AAAGCAGGTC CATCCGGACA CTGGCATCTC TTCGAAAGCT 
ATGAGCATTA TGAATTCCTT CGTCACTGAT ATCTTTGAGC GTATAGCGAG CGAGGCATCA 
CGTTTGGCTC ACTACAGCAA GCGCTCCACC ATTTCTTCCA GAGAGATTCA GACAGCAGTG 
CGCTTGCTAC TGCCGGGAGA GCTGGCTAAA CATGCTGTGT CTGAGGGCAC CAAGGCTGTC 
ACTAAGTACA CCAGCTCCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733759.1
CDS	133..516
Translation	MPEVSSKGATISKKGFKKAVVKTQKKEGKKRKRTRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYSKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

Target ORF information:

RefSeq Version	NM_170610.3
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B clustered histone 1 (H2BC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170610.3

ORF Insert Sequence:

ATGCCGGAGG TGTCATCTAA AGGTGCTACC ATTTCCAAGA AGGGCTTTAA GAAAGCTGTC 
GTTAAGACCC AGAAAAAGGA AGGCAAAAAG CGCAAGAGGA CCCGTAAGGA GAGTTATTCT 
ATTTACATCT ACAAAGTGCT AAAGCAGGTC CATCCGGACA CTGGCATCTC TTCGAAAGCT 
ATGAGCATTA TGAATTCCTT CGTCACTGAT ATCTTTGAGC GTATAGCGAG CGAGGCATCA 
CGTTTGGCTC ACTACAGCAA GCGCTCCACC ATTTCTTCCA GAGAGATTCA GACAGCAGTG 
CGCTTGCTAC TGCCGGGAGA GCTGGCTAAA CATGCTGTGT CTGAGGGCAC CAAGGCTGTC 
ACTAAGTACA CCAGCTCCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The inhibition of lactate dehydrogenase A hinders the transcription of histone 2B gene independently from the block of aerobic glycolysis.
Biochemical and biophysical research communications485(4)742-745(2017 Apr)
Brighenti E,Carnicelli D,Brigotti M,Fiume L

Structural analyses of the nucleosome complexes with human testis-specific histone variants, hTh2a and hTh2b.
Biophysical chemistry22141-48(2017 Feb)
Padavattan S,Thiruselvam V,Shinagawa T,Hasegawa K,Kumasaka T,Ishii S,Kumarevel T

Nick Your DNA, Mark Your Chromatin.
Molecular cell64(1)7-9(2016 Oct)
Nardozza AP,Ladurner AG

Two Histone Variants TH2A and TH2B Enhance Human Induced Pluripotent Stem Cell Generation.
Stem cells and development25(3)251-8(2016 Feb)
Huynh LM,Shinagawa T,Ishii S

Hypoacetylation, hypomethylation, and dephosphorylation of H2B histones and excessive histone deacetylase activity in DU-145 prostate cancer cells.
Journal of hematology & oncology93(2016 Jan)
Cang S,Xu X,Ma Y,Liu D,Chiao JW

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HIST1H2BA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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