GAST cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAST
Entrez Gene ID	2520
Full Name	gastrin
Synonyms	GAS
General protein information	Preferred Names gastrin Names gastrin preprogastrin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008].
Disorder MIM:	137250

mRNA and Protein(s)

mRNA	Protein	Name
NM_000805.5	NP_000796.1	gastrin preproprotein
NM_000805.4	NP_000796.1	gastrin preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gast	NP_034387.3
Bos taurus (cattle)	GAST	NP_776340.1
Homo sapiens (human)	GAST	NP_000796.1
Canis lupus familiaris (dog)	GAST	XP_003435286.1
Rattus norvegicus (Norway rat)	Gast	NP_036981.1
Pan troglodytes (chimpanzee)	GAST	XP_523651.2
	GAST	XP_001107333.1

Gene Ontology
Bibliography

Related articles in PubMed

[Expressions of gastrin and apoptosis-associated proteins involved in mitochondrial pathway in gastric cancer tissues and the clinical significance].
Feng L, Zhu J, Sun W, Zhao J, Liu Y
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(11)1557-1561(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis.
Title: Gastrin induces parathyroid hormone-like hormone expression in gastric parietal cells.

Gastrin is expressed in both insulin(+) and somatostatin(+) cells in adults with type 2 diabetes.
Title: Pancreatic ?-Cells Express the Fetal Islet Hormone Gastrin in Rodent and Human Diabetes.

Results demonstrate that gastrin increases the expression of the autophagy markers MAP1LC3B-II and SQSTM1 in gastric adenocarcinoma cells. Gastrin induces autophagy via activation of the STK11-PRKAA2-ULK1 and that this signaling pathway is involved in increased migration and cell survival.
Title: Gastrin activates autophagy and increases migration and survival of gastric adenocarcinoma cells.

Gastrin induced ABCG2 expression by activating NF-kappaB and thereby modulated the side population proportion, tumor cell metastatic potential and invasion activity in pancreatic cancer.
Title: Gastrin regulates ABCG2 to promote the migration, invasion and side populations in pancreatic cancer cells via activation of NF-?B signaling.

CTGF is overexpressed in gastric cancer and adjacent tissue compared to normal gastric tissue. Gastrin induces expression of CTGF in gastric epithelial cells.
Title: Connective tissue growth factor is activated by gastrin and involved in gastrin-induced migration and invasion.

The following GAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu55892	NM_000805.4 Latest version!	Homo sapiens gastrin (GAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107224	NM_000805.5 Latest version!	Homo sapiens gastrin (GAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAST ( NM_000805.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000796.1 Homo sapiens gastrin (GAST), mRNA.

Price & Availability↑

CloneID	OHu55892
Clone ID Related Accession (Same CDS sequence)	NM_000805.4
Accession Version	NM_000805.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	gastrin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM768762.1, BC069724.1 and BM768483.1. On Oct 15, 2011 this sequence version replaced NM_000805.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM854691.1, BM768762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCGAC TATGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000796.1
CDS	68..373
Translation	MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN

Target ORF information:

RefSeq Version	NM_000805.4
Organism	Homo sapiens(human)
Definition	Homo sapiens gastrin (GAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000805.4

ORF Insert Sequence:

ATGCAGCGAC TATGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAST ( NM_000805.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000796.1 Homo sapiens gastrin (GAST), mRNA.

Price & Availability↑

CloneID	OHu107224
Clone ID Related Accession (Same CDS sequence)	NM_000805.5
Accession Version	NM_000805.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	gastrin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130686.15 and AC109319.17. On Nov 22, 2018 this sequence version replaced NM_000805.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM768289.1, BM768748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329402.4/ ENSP00000331358.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCGAC TGTGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000796.1
CDS	63..368
Translation	MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN

Target ORF information:

RefSeq Version	NM_000805.5
Organism	Homo sapiens(human)
Definition	Homo sapiens gastrin (GAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000805.5

ORF Insert Sequence:

ATGCAGCGAC TGTGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Expressions of gastrin and apoptosis-associated proteins involved in mitochondrial pathway in gastric cancer tissues and the clinical significance].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(11)1557-1561(2017 Nov)
Feng L,Zhu J,Sun W,Zhao J,Liu Y

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GAST cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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