SH3BP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SH3BP1
Entrez Gene ID	23616
Full Name	SH3 domain binding protein 1
Synonyms	ARHGAP43
General protein information	Preferred Names SH3 domain binding protein 1 Names SH3 domain-binding protein 1 3BP-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	This gene encodes a member of the Rho GTPase activating protein (RhoGAP) family. The encoded protein regulates Rac signaling and plays a role in cytoskeletal dynamics, cell motility and epithelial junction formation. This protein's association with the exocyst complex, which tethers secretory vesicles to the plasma membrane, has been demonstrated to be important in cell motility. In a distinct complex, this protein has been shown to regulate epithelial junction formation and morphogenesis. By interacting with the Plexin-D1 cell surface receptor, this protein mediates changes in the cytoskeleton in response to semaphorin binding. This protein may promote metastasis in human liver cancer cells and tissues. [provided by RefSeq, Mar 2017].
Disorder MIM:	617368

mRNA and Protein(s)

mRNA	Protein	Name
NM_018957.4	NP_061830.3	SH3 domain-binding protein 1 isoform 1
NM_001350055.1	NP_001336984.1	SH3 domain-binding protein 1 isoform 2
NM_018957.5	NP_061830.3	SH3 domain-binding protein 1 isoform 1
NM_001350055.2	NP_001336984.1	SH3 domain-binding protein 1 isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SH3BP1	XP_002687966.1
Homo sapiens (human)	SH3BP1	NP_061830.3
Pan troglodytes (chimpanzee)	SH3BP1	XP_515119.2
	SH3BP1	XP_002798436.1
Mus musculus (house mouse)	Sh3bp1	NP_033190.2
Gallus gallus (chicken)	SH3BP1	XP_428489.3
Canis lupus familiaris (dog)	SH3BP1	XP_538386.3
Danio rerio (zebrafish)	sh3bp1	XP_001345357.4
Rattus norvegicus (Norway rat)	Sh3bp1	NP_001165452.1
Xenopus tropicalis (tropical clawed frog)	sh3bp1	XP_004913943.1

Gene Ontology
Bibliography

Related articles in PubMed

SH3BP1-induced Rac-Wave2 pathway activation regulates cervical cancer cell migration, invasion, and chemoresistance to cisplatin.
Wang J, Feng Y, Chen X, Du Z, Jiang S, Ma S, Zou W
Journal of cellular biochemistry119(2)1733-1745(2018 Feb)

SH3-domain binding protein 1 in the tumor microenvironment promotes hepatocellular carcinoma metastasis through WAVE2 pathway.
Tao Y, Hu K, Tan F, Zhang S, Zhou M, Luo J, Wang Z
Oncotarget7(14)18356-70(2016 Apr)

Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.
Schlam D, Bagshaw RD, Freeman SA, Collins RF, Pawson T, Fairn GD, Grinstein S
Nature communications68623(2015 Oct)

An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling.
Tata A, Stoppel DC, Hong S, Ben-Zvi A, Xie T, Gu C
The Journal of cell biology205(4)573-90(2014 May)

A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac1 inactivation.
Huang TY, Michael S, Xu T, Sarkeshik A, Moresco JJ, Yates JR 3rd, Masliah E, Bokoch GM, DerMardirossian C
Molecular biology of the cell24(3)194-209(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SH3BP1 promoted VEGF secretion via Rac1-WAVE2 signaling, so as to exert an augmentation on cell invasion and microvessel formation.
Title: SH3-domain binding protein 1 in the tumor microenvironment promotes hepatocellular carcinoma metastasis through WAVE2 pathway.

The identification and characterization of SH3BP1 as a novel downstream effector of Sema3E-PlexinD1 provides an explanation for how extracellular signals are translated into cytoskeletal changes and unique cell behavior.
Title: An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling.

Splicing of SH3BP1 and CIN gene loci produces the novel brain specific splice variant BGIN.
Title: A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac1 inactivation.

Epithelial junction formation and morphogenesis require a dual activity complex, containing SH3BP1 and CapZ, that is recruited to sites of active membrane remodeling to guide Cdc42 signaling and cytoskeletal dynamics.
Title: Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex.

The following SH3BP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SH3BP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02369	NM_018957.5 Latest version!	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OHu101146	NM_001350055.2 Latest version!	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu02369	NM_018957.4	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OHu101146	NM_001350055.1	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SH3BP1 ( NM_018957.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061830.3 Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02369
Clone ID Related Accession (Same CDS sequence)	NM_018957.4 , NM_018957.5
Accession Version	NM_018957.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	SH3 domain-binding protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126873.1, BX465097.2, CR456576.1, BQ005545.1, AA748769.1 and HY340971.1. This sequence is a reference standard in the RefSeqGene project. On Apr 1, 2017 this sequence version replaced NM_018957.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: CR456576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGACCAAGCG CCCGGCGCCA GCCCGGCCCA CCATGCCGCC CCCCCAGGTC 
TCCGGCTCCC GCTCCTCCCC TCCAGCCCCG CCCTTGCCCC CTGGCTCTGG CAGCCCTGGG 
ACCCCCCAAG CCCTGCCCCG ACGTCTGGTT GGCAGCAGCC TCCGAGCCCC CACAGTGCCA 
CCCCCGTTAC CCCCCACACC CCCTCAGCCT GCCCGGCGCC AAAGCCGGCG TTCACCAGCC 
TCCCCCAGCC CGGCCTCCCC AGGTCCAGCC TCCCCCAGCC CAGTCTCTTT GAGTAACCCT 
GCACAGGTGG ACCTGGGGGC TGCCACAGCA GAGGGAGGAG CCCCTGAGGC TATCAGTGGG 
GTCCCCACTC CCCCAGCTAT CCCCCCTCAG CCCCGCCCCA GGAGCCTTGC CTCAGAGACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061830.3
CDS	314..2419
Translation	MMKRQLHRMRQLAQTGSLGRTPETAEFLGEDLLQVEQRLEPAKRAAHNIHKRLQACLQGQSGADMDKRVKKLPLMALSTTMAESFKELDPDSSMGKALEMSCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVSDWNTLKSRLSQATKNSGSSQGLGGSPGSHSHTTMANKVETLKEEEEELKRKVEQCRDEYLADLYHFVTKEDSYANYFIRLLEIQADYHRRSLSSLDTALAELRENHGQADHSPSMTATHFPRVYGVSLATHLQELGREIALPIEACVMMLLSEGMKEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLMTFDLYDDWMRAASLKEPGARLQALQEVCSRLPPENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPPRPASPKVTRSPPETAAPVEDMARRTKRPAPARPTMPPPQVSGSRSSPPAPPLPPGSGSPGTPQALPRRLVGSSLRAPTVPPPLPPTPPQPARRQSRRSPASPSPASPGPASPSPVSLSNPAQVDLGAATAEGGAPEAISGVPTPPAIPPQPRPRSLASETN

Target ORF information:

RefSeq Version	NM_018957.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018957.4

ORF Insert Sequence:

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGACCAAGCG CCCGGCGCCA GCCCGGCCCA CCATGCCGCC CCCCCAGGTC 
TCCGGCTCCC GCTCCTCCCC TCCAGCCCCG CCCTTGCCCC CTGGCTCTGG CAGCCCTGGG 
ACCCCCCAAG CCCTGCCCCG ACGTCTGGTT GGCAGCAGCC TCCGAGCCCC CACAGTGCCA 
CCCCCGTTAC CCCCCACACC CCCTCAGCCT GCCCGGCGCC AAAGCCGGCG TTCACCAGCC 
TCCCCCAGCC CGGCCTCCCC AGGTCCAGCC TCCCCCAGCC CAGTCTCTTT GAGTAACCCT 
GCACAGGTGG ACCTGGGGGC TGCCACAGCA GAGGGAGGAG CCCCTGAGGC TATCAGTGGG 
GTCCCCACTC CCCCAGCTAT CCCCCCTCAG CCCCGCCCCA GGAGCCTTGC CTCAGAGACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3BP1 ( NM_018957.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061830.3 Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02369
Clone ID Related Accession (Same CDS sequence)	NM_018957.4 , NM_018957.5
Accession Version	NM_018957.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	SH3 domain-binding protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126873.1, BX465097.2, CR456576.1, BQ005545.1, AA748769.1 and HY340971.1. This sequence is a reference standard in the RefSeqGene project. On May 11, 2018 this sequence version replaced NM_018957.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR456576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGACCAAGCG CCCGGCGCCA GCCCGGCCCA CCATGCCGCC CCCCCAGGTC 
TCCGGCTCCC GCTCCTCCCC TCCAGCCCCG CCCTTGCCCC CTGGCTCTGG CAGCCCTGGG 
ACCCCCCAAG CCCTGCCCCG ACGTCTGGTT GGCAGCAGCC TCCGAGCCCC CACAGTGCCA 
CCCCCGTTAC CCCCCACACC CCCTCAGCCT GCCCGGCGCC AAAGCCGGCG TTCACCAGCC 
TCCCCCAGCC CGGCCTCCCC AGGTCCAGCC TCCCCCAGCC CAGTCTCTTT GAGTAACCCT 
GCACAGGTGG ACCTGGGGGC TGCCACAGCA GAGGGAGGAG CCCCTGAGGC TATCAGTGGG 
GTCCCCACTC CCCCAGCTAT CCCCCCTCAG CCCCGCCCCA GGAGCCTTGC CTCAGAGACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061830.3
CDS	120..2225
Translation	MMKRQLHRMRQLAQTGSLGRTPETAEFLGEDLLQVEQRLEPAKRAAHNIHKRLQACLQGQSGADMDKRVKKLPLMALSTTMAESFKELDPDSSMGKALEMSCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVSDWNTLKSRLSQATKNSGSSQGLGGSPGSHSHTTMANKVETLKEEEEELKRKVEQCRDEYLADLYHFVTKEDSYANYFIRLLEIQADYHRRSLSSLDTALAELRENHGQADHSPSMTATHFPRVYGVSLATHLQELGREIALPIEACVMMLLSEGMKEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLMTFDLYDDWMRAASLKEPGARLQALQEVCSRLPPENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPPRPASPKVTRSPPETAAPVEDMARRTKRPAPARPTMPPPQVSGSRSSPPAPPLPPGSGSPGTPQALPRRLVGSSLRAPTVPPPLPPTPPQPARRQSRRSPASPSPASPGPASPSPVSLSNPAQVDLGAATAEGGAPEAISGVPTPPAIPPQPRPRSLASETN

Target ORF information:

RefSeq Version	NM_018957.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018957.5

ORF Insert Sequence:

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGACCAAGCG CCCGGCGCCA GCCCGGCCCA CCATGCCGCC CCCCCAGGTC 
TCCGGCTCCC GCTCCTCCCC TCCAGCCCCG CCCTTGCCCC CTGGCTCTGG CAGCCCTGGG 
ACCCCCCAAG CCCTGCCCCG ACGTCTGGTT GGCAGCAGCC TCCGAGCCCC CACAGTGCCA 
CCCCCGTTAC CCCCCACACC CCCTCAGCCT GCCCGGCGCC AAAGCCGGCG TTCACCAGCC 
TCCCCCAGCC CGGCCTCCCC AGGTCCAGCC TCCCCCAGCC CAGTCTCTTT GAGTAACCCT 
GCACAGGTGG ACCTGGGGGC TGCCACAGCA GAGGGAGGAG CCCCTGAGGC TATCAGTGGG 
GTCCCCACTC CCCCAGCTAT CCCCCCTCAG CCCCGCCCCA GGAGCCTTGC CTCAGAGACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3BP1 ( NM_001350055.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336984.1 Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101146
Clone ID Related Accession (Same CDS sequence)	NM_001350055.1 , NM_001350055.2
Accession Version	NM_001350055.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	SH3 domain-binding protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z83844.5 and HY340971.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region and differs in the 3' UTR compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.585993.1, SRR1163655.40355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGAGTCCTAG GGGAGCCACC GGAAGGAAGG AGAGGTTTGC CTGCTCCTAC 
GGGACTGATT CTTCTCTTGC CGACATGTTT TTTGTAAGGC TGGTAAATAA ATTATTTTGG 
ACAAAACTGG AGCAGCTGCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336984.1
CDS	314..2140
Translation	MMKRQLHRMRQLAQTGSLGRTPETAEFLGEDLLQVEQRLEPAKRAAHNIHKRLQACLQGQSGADMDKRVKKLPLMALSTTMAESFKELDPDSSMGKALEMSCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVSDWNTLKSRLSQATKNSGSSQGLGGSPGSHSHTTMANKVETLKEEEEELKRKVEQCRDEYLADLYHFVTKEDSYANYFIRLLEIQADYHRRSLSSLDTALAELRENHGQADHSPSMTATHFPRVYGVSLATHLQELGREIALPIEACVMMLLSEGMKEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLMTFDLYDDWMRAASLKEPGARLQALQEVCSRLPPENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPPRPASPKVTRSPPETAAPVEDMARRSPRGATGRKERFACSYGTDSSLADMFFVRLVNKLFWTKLEQLPK

Target ORF information:

RefSeq Version	NM_001350055.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350055.1

ORF Insert Sequence:

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGAGTCCTAG GGGAGCCACC GGAAGGAAGG AGAGGTTTGC CTGCTCCTAC 
GGGACTGATT CTTCTCTTGC CGACATGTTT TTTGTAAGGC TGGTAAATAA ATTATTTTGG 
ACAAAACTGG AGCAGCTGCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3BP1 ( NM_001350055.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336984.1 Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101146
Clone ID Related Accession (Same CDS sequence)	NM_001350055.1 , NM_001350055.2
Accession Version	NM_001350055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	SH3 domain-binding protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z83844.5 and HY340971.1. On May 31, 2019 this sequence version replaced NM_001350055.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region and differs in the 3' UTR compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.585993.1, SRR1163655.40355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGAGTCCTAG GGGAGCCACC GGAAGGAAGG AGAGGTTTGC CTGCTCCTAC 
GGGACTGATT CTTCTCTTGC CGACATGTTT TTTGTAAGGC TGGTAAATAA ATTATTTTGG 
ACAAAACTGG AGCAGCTGCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336984.1
CDS	120..1946
Translation	MMKRQLHRMRQLAQTGSLGRTPETAEFLGEDLLQVEQRLEPAKRAAHNIHKRLQACLQGQSGADMDKRVKKLPLMALSTTMAESFKELDPDSSMGKALEMSCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVSDWNTLKSRLSQATKNSGSSQGLGGSPGSHSHTTMANKVETLKEEEEELKRKVEQCRDEYLADLYHFVTKEDSYANYFIRLLEIQADYHRRSLSSLDTALAELRENHGQADHSPSMTATHFPRVYGVSLATHLQELGREIALPIEACVMMLLSEGMKEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLMTFDLYDDWMRAASLKEPGARLQALQEVCSRLPPENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPPRPASPKVTRSPPETAAPVEDMARRSPRGATGRKERFACSYGTDSSLADMFFVRLVNKLFWTKLEQLPK

Target ORF information:

RefSeq Version	NM_001350055.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain binding protein 1 (SH3BP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350055.2

ORF Insert Sequence:

ATGATGAAGA GGCAGCTGCA CCGCATGCGG CAGCTGGCCC AGACGGGCAG CTTGGGACGC 
ACCCCGGAGA CCGCTGAGTT CCTGGGTGAG GACCTGCTGC AGGTAGAACA GCGGCTGGAG 
CCGGCCAAGC GGGCAGCCCA CAACATCCAC AAGCGGCTGC AGGCCTGTCT GCAGGGCCAG 
AGCGGGGCAG ACATGGACAA GCGGGTGAAG AAGCTTCCCC TCATGGCTCT GTCCACCACG 
ATGGCTGAGA GCTTCAAGGA GCTGGACCCT GATTCCAGCA TGGGGAAGGC CTTGGAGATG 
AGCTGTGCCA TCCAGAATCA GCTGGCCCGC ATCCTGGCCG AGTTTGAGAT GACCCTGGAG 
AGGGACGTCC TGCAGCCACT CAGCAGGCTG AGTGAGGAGG AGCTGCCAGC CATCCTCAAA 
CACAAGAAAA GCCTCCAGAA GCTCGTGTCC GACTGGAACA CACTCAAGAG CAGGCTCAGT 
CAGGCAACCA AGAATTCAGG CAGCAGTCAA GGCCTAGGAG GCAGCCCGGG TAGTCACAGC 
CATACGACCA TGGCCAACAA GGTGGAGACG CTGAAGGAGG AGGAGGAGGA GCTGAAGAGG 
AAAGTGGAGC AATGCAGGGA CGAGTACTTG GCTGACCTGT ACCACTTTGT TACCAAGGAG 
GACTCCTATG CCAACTACTT CATTCGTCTC CTGGAGATTC AGGCCGATTA CCATCGCAGG 
TCACTGAGCT CGCTGGACAC AGCCCTGGCT GAGCTGAGGG AGAACCACGG CCAAGCAGAC 
CACTCCCCTT CGATGACAGC CACCCACTTC CCCAGGGTGT ATGGGGTGTC GCTGGCAACC 
CACCTGCAAG AGCTGGGCCG GGAGATTGCC CTGCCCATCG AGGCCTGCGT CATGATGCTG 
CTTTCTGAGG GCATGAAGGA AGAGGGTCTC TTCCGTCTGG CTGCTGGGGC CTCGGTGCTG 
AAGCGTCTCA AGCAGACAAT GGCCTCGGAC CCCCACAGCC TGGAGGAGTT CTGCTCCGAC 
CCGCACGCTG TGGCAGGTGC CCTCAAGTCC TATCTGCGGG AGCTGCCAGA GCCTCTGATG 
ACCTTCGACC TCTATGATGA CTGGATGAGG GCAGCCAGCC TGAAGGAGCC AGGGGCCCGG 
CTGCAGGCCC TCCAAGAGGT GTGCAGCCGC CTACCCCCCG AGAACCTCAG CAACCTCAGG 
TACCTGATGA AGTTCCTGGC ACGGCTGGCC GAGGAGCAGG AGGTGAACAA GATGACACCC 
AGCAACATCG CCATAGTCCT GGGACCCAAC TTGCTGTGGC CACCTGAGAA AGAAGGGGAC 
CAGGCCCAGC TGGATGCAGC CTCCGTGTCT TCCATCCAGG TGGTGGGCGT CGTCGAGGCG 
CTGATCCAGA GCGCAGACAC CCTCTTCCCT GGAGACATCA ACTTCAACGT GTCAGGCCTC 
TTCTCAGCTG TTACCCTCCA GGACACAGTC AGTGACAGGC TGGCCTCTGA GGAACTTCCG 
TCCACTGCCG TGCCCACCCC AGCCACCACC CCGGCTCCGG CTCCGGCTCC AGCTCCAGCT 
CCGGCCCCAG CCTTGGCTTC AGCAGCTACC AAGGAAAGGA CAGAGTCTGA GGTGCCTCCC 
AGACCAGCCT CCCCCAAGGT CACCAGGAGT CCCCCGGAGA CAGCTGCCCC AGTGGAGGAC 
ATGGCTCGGA GGAGTCCTAG GGGAGCCACC GGAAGGAAGG AGAGGTTTGC CTGCTCCTAC 
GGGACTGATT CTTCTCTTGC CGACATGTTT TTTGTAAGGC TGGTAAATAA ATTATTTTGG 
ACAAAACTGG AGCAGCTGCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SH3BP1-induced Rac-Wave2 pathway activation regulates cervical cancer cell migration, invasion, and chemoresistance to cisplatin.
Journal of cellular biochemistry119(2)1733-1745(2018 Feb)
Wang J,Feng Y,Chen X,Du Z,Jiang S,Ma S,Zou W

SH3-domain binding protein 1 in the tumor microenvironment promotes hepatocellular carcinoma metastasis through WAVE2 pathway.
Oncotarget7(14)18356-70(2016 Apr)
Tao Y,Hu K,Tan F,Zhang S,Zhou M,Luo J,Wang Z

Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.
Nature communications68623(2015 Oct)
Schlam D,Bagshaw RD,Freeman SA,Collins RF,Pawson T,Fairn GD,Grinstein S

An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling.
The Journal of cell biology205(4)573-90(2014 May)
Tata A,Stoppel DC,Hong S,Ben-Zvi A,Xie T,Gu C

A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac1 inactivation.
Molecular biology of the cell24(3)194-209(2013 Feb)
Huang TY,Michael S,Xu T,Sarkeshik A,Moresco JJ,Yates JR 3rd,Masliah E,Bokoch GM,DerMardirossian C

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SH3BP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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