DDX58 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX58
Entrez Gene ID	23586
Full Name	DExD/H-box helicase 58
Synonyms	RIG-I,RIGI,RLR-1,SGMRT2
General protein information	Preferred Names DExD/H-box helicase 58 Names probable ATP-dependent RNA helicase DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 DEAD box protein 58 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RNA helicase RIG-I retinoic acid-inducible gene 1 protein retinoic acid-inducible gene I protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p21.1
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008].
Disorder MIM:	609631
Disorder Html:	Singleton-Merten syndrome 2, 616298 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014314.4	NP_055129.2	probable ATP-dependent RNA helicase DDX58
NM_014314.3	NP_055129.2	probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx58	NP_766277.3
Pan troglodytes (chimpanzee)	DDX58	XP_001156662.1
Caenorhabditis elegans (roundworm)	drh-1	NP_501018.1
Homo sapiens (human)	DDX58	NP_055129.2
Canis lupus familiaris (dog)	DDX58	XP_003639433.1
Bos taurus (cattle)	DDX58	XP_002689526.2
Xenopus tropicalis (tropical clawed frog)	ddx58	XP_002935717.2
	DDX58	NP_001036133.1

Gene Ontology

The following DDX58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25414	NM_014314.4 Latest version!	Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu25414	NM_014314.3	Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX58 ( NM_014314.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055129.2 Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.

Price & Availability↑

CloneID	OHu25414
Clone ID Related Accession (Same CDS sequence)	NM_014314.4 , NM_014314.3
Accession Version	NM_014314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN998145.1, AF038963.1, BC070029.1, AL161783.20 and AA862613.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014314.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.175842.1, SRR1660803.37851.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379883.3/ ENSP00000369213.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCACCG AGCAGCGACG CAGCCTGCAA GCCTTCCAGG ATTATATCCG GAAGACCCTG 
GACCCTACCT ACATCCTGAG CTACATGGCC CCCTGGTTTA GGGAGGAAGA GGTGCAGTAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCACACTTTT TCTCAAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGTGGCTTTT TGGATGCCCT AGACCATGCA 
GGTTATTCTG GACTTTATGA AGCCATTGAA AGTTGGGATT TCAAAAAAAT TGAAAAGTTG 
GAGGAGTATA GATTACTTTT AAAACGTTTA CAACCAGAAT TTAAAACCAG AATTATCCCA 
ACCGATATCA TTTCTGATCT GTCTGAATGT TTAATTAATC AGGAATGTGA AGAAATTCTA 
CAGATTTGCT CTACTAAGGG GATGATGGCA GGTGCAGAGA AATTGGTGGA ATGCCTTCTC 
AGATCAGACA AGGAAAACTG GCCCAAAACT TTGAAACTTG CTTTGGAGAA AGAAAGGAAC 
AAGTTCAGTG AACTGTGGAT TGTAGAGAAA GGTATAAAAG ATGTTGAAAC AGAAGATCTT 
GAGGATAAGA TGGAAACTTC TGACATACAG ATTTTCTACC AAGAAGATCC AGAATGCCAG 
AATCTTAGTG AGAATTCATG TCCACCTTCA GAAGTGTCTG ATACAAACTT GTACAGCCCA 
TTTAAACCAA GAAATTACCA ATTAGAGCTT GCTTTGCCTG CTATGAAAGG AAAAAACACA 
ATAATATGTG CTCCTACAGG TTGTGGAAAA ACCTTTGTTT CACTGCTTAT ATGTGAACAT 
CATCTTAAAA AATTCCCACA AGGACAAAAG GGGAAAGTTG TCTTTTTTGC GAATCAGATC 
CCAGTGTATG AACAGCAGAA ATCTGTATTC TCAAAATACT TTGAAAGACA TGGGTATAGA 
GTTACAGGCA TTTCTGGAGC AACAGCTGAG AATGTCCCAG TGGAACAGAT TGTTGAGAAC 
AATGACATCA TCATTTTAAC TCCACAGATT CTTGTGAACA ACCTTAAAAA GGGAACGATT 
CCATCACTAT CCATCTTTAC TTTGATGATA TTTGATGAAT GCCACAACAC TAGTAAACAA 
CACCCGTACA ATATGATCAT GTTTAATTAT CTAGATCAGA AACTTGGAGG ATCTTCAGGC 
CCACTGCCCC AGGTCATTGG GCTGACTGCC TCGGTTGGTG TTGGGGATGC CAAAAACACA 
GATGAAGCCT TGGATTATAT CTGCAAGCTG TGTGCTTCTC TTGATGCGTC AGTGATAGCA 
ACAGTCAAAC ACAATCTGGA GGAACTGGAG CAAGTTGTTT ATAAGCCCCA GAAGTTTTTC 
AGGAAAGTGG AATCACGGAT TAGCGACAAA TTTAAATACA TCATAGCTCA GCTGATGAGG 
GACACAGAGA GTCTGGCAAA GAGAATCTGC AAAGACCTCG AAAACTTATC TCAAATTCAA 
AATAGGGAAT TTGGAACACA GAAATATGAA CAATGGATTG TTACAGTTCA GAAAGCATGC 
ATGGTGTTCC AGATGCCAGA CAAAGATGAA GAGAGCAGGA TTTGTAAAGC CCTGTTTTTA 
TACACTTCAC ATTTGCGGAA ATATAATGAT GCCCTCATTA TCAGTGAGCA TGCACGAATG 
AAAGATGCTC TGGATTACTT GAAAGACTTC TTCAGCAATG TCCGAGCAGC AGGATTCGAT 
GAGATTGAGC AAGATCTTAC TCAGAGATTT GAAGAAAAGC TGCAGGAACT AGAAAGTGTT 
TCCAGGGATC CCAGCAATGA GAATCCTAAA CTTGAAGACC TCTGCTTCAT CTTACAAGAA 
GAGTACCACT TAAACCCAGA GACAATAACA ATTCTCTTTG TGAAAACCAG AGCACTTGTG 
GACGCTTTAA AAAATTGGAT TGAAGGAAAT CCTAAACTCA GTTTTCTAAA ACCTGGCATA 
TTGACTGGAC GTGGCAAAAC AAATCAGAAC ACAGGAATGA CCCTCCCGGC ACAGAAGTGT 
ATATTGGATG CATTCAAAGC CAGTGGAGAT CACAATATTC TGATTGCCAC CTCAGTTGCT 
GATGAAGGCA TTGACATTGC ACAGTGCAAT CTTGTCATCC TTTATGAGTA TGTGGGCAAT 
GTCATCAAAA TGATCCAAAC CAGAGGCAGA GGAAGAGCAA GAGGTAGCAA GTGCTTCCTT 
CTGACTAGTA ATGCTGGTGT AATTGAAAAA GAACAAATAA ACATGTACAA AGAAAAAATG 
ATGAATGACT CTATTTTACG CCTTCAGACA TGGGACGAAG CAGTATTTAG GGAAAAGATT 
CTGCATATAC AGACTCATGA AAAATTCATC AGAGATAGTC AAGAAAAACC AAAACCTGTA 
CCTGATAAGG AAAATAAAAA ACTGCTCTGC AGAAAGTGCA AAGCCTTGGC ATGTTACACA 
GCTGACGTAA GAGTGATAGA GGAATGCCAT TACACTGTGC TTGGAGATGC TTTTAAGGAA 
TGCTTTGTGA GTAGACCACA TCCCAAGCCA AAGCAGTTTT CAAGTTTTGA AAAAAGAGCA 
AAGATATTCT GTGCCCGACA GAACTGCAGC CATGACTGGG GAATCCATGT GAAGTACAAG 
ACATTTGAGA TTCCAGTTAT AAAAATTGAA AGTTTTGTGG TGGAGGATAT TGCAACTGGA 
GTTCAGACAC TGTACTCGAA GTGGAAGGAC TTTCATTTTG AGAAGATACC ATTTGATCCA 
GCAGAAATGT CCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055129.2
CDS	31..2808
Translation	MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK

Target ORF information:

RefSeq Version	NM_014314.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014314.4

ORF Insert Sequence:

ATGACCACCG AGCAGCGACG CAGCCTGCAA GCCTTCCAGG ATTATATCCG GAAGACCCTG 
GACCCTACCT ACATCCTGAG CTACATGGCC CCCTGGTTTA GGGAGGAAGA GGTGCAGTAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCACACTTTT TCTCAAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGTGGCTTTT TGGATGCCCT AGACCATGCA 
GGTTATTCTG GACTTTATGA AGCCATTGAA AGTTGGGATT TCAAAAAAAT TGAAAAGTTG 
GAGGAGTATA GATTACTTTT AAAACGTTTA CAACCAGAAT TTAAAACCAG AATTATCCCA 
ACCGATATCA TTTCTGATCT GTCTGAATGT TTAATTAATC AGGAATGTGA AGAAATTCTA 
CAGATTTGCT CTACTAAGGG GATGATGGCA GGTGCAGAGA AATTGGTGGA ATGCCTTCTC 
AGATCAGACA AGGAAAACTG GCCCAAAACT TTGAAACTTG CTTTGGAGAA AGAAAGGAAC 
AAGTTCAGTG AACTGTGGAT TGTAGAGAAA GGTATAAAAG ATGTTGAAAC AGAAGATCTT 
GAGGATAAGA TGGAAACTTC TGACATACAG ATTTTCTACC AAGAAGATCC AGAATGCCAG 
AATCTTAGTG AGAATTCATG TCCACCTTCA GAAGTGTCTG ATACAAACTT GTACAGCCCA 
TTTAAACCAA GAAATTACCA ATTAGAGCTT GCTTTGCCTG CTATGAAAGG AAAAAACACA 
ATAATATGTG CTCCTACAGG TTGTGGAAAA ACCTTTGTTT CACTGCTTAT ATGTGAACAT 
CATCTTAAAA AATTCCCACA AGGACAAAAG GGGAAAGTTG TCTTTTTTGC GAATCAGATC 
CCAGTGTATG AACAGCAGAA ATCTGTATTC TCAAAATACT TTGAAAGACA TGGGTATAGA 
GTTACAGGCA TTTCTGGAGC AACAGCTGAG AATGTCCCAG TGGAACAGAT TGTTGAGAAC 
AATGACATCA TCATTTTAAC TCCACAGATT CTTGTGAACA ACCTTAAAAA GGGAACGATT 
CCATCACTAT CCATCTTTAC TTTGATGATA TTTGATGAAT GCCACAACAC TAGTAAACAA 
CACCCGTACA ATATGATCAT GTTTAATTAT CTAGATCAGA AACTTGGAGG ATCTTCAGGC 
CCACTGCCCC AGGTCATTGG GCTGACTGCC TCGGTTGGTG TTGGGGATGC CAAAAACACA 
GATGAAGCCT TGGATTATAT CTGCAAGCTG TGTGCTTCTC TTGATGCGTC AGTGATAGCA 
ACAGTCAAAC ACAATCTGGA GGAACTGGAG CAAGTTGTTT ATAAGCCCCA GAAGTTTTTC 
AGGAAAGTGG AATCACGGAT TAGCGACAAA TTTAAATACA TCATAGCTCA GCTGATGAGG 
GACACAGAGA GTCTGGCAAA GAGAATCTGC AAAGACCTCG AAAACTTATC TCAAATTCAA 
AATAGGGAAT TTGGAACACA GAAATATGAA CAATGGATTG TTACAGTTCA GAAAGCATGC 
ATGGTGTTCC AGATGCCAGA CAAAGATGAA GAGAGCAGGA TTTGTAAAGC CCTGTTTTTA 
TACACTTCAC ATTTGCGGAA ATATAATGAT GCCCTCATTA TCAGTGAGCA TGCACGAATG 
AAAGATGCTC TGGATTACTT GAAAGACTTC TTCAGCAATG TCCGAGCAGC AGGATTCGAT 
GAGATTGAGC AAGATCTTAC TCAGAGATTT GAAGAAAAGC TGCAGGAACT AGAAAGTGTT 
TCCAGGGATC CCAGCAATGA GAATCCTAAA CTTGAAGACC TCTGCTTCAT CTTACAAGAA 
GAGTACCACT TAAACCCAGA GACAATAACA ATTCTCTTTG TGAAAACCAG AGCACTTGTG 
GACGCTTTAA AAAATTGGAT TGAAGGAAAT CCTAAACTCA GTTTTCTAAA ACCTGGCATA 
TTGACTGGAC GTGGCAAAAC AAATCAGAAC ACAGGAATGA CCCTCCCGGC ACAGAAGTGT 
ATATTGGATG CATTCAAAGC CAGTGGAGAT CACAATATTC TGATTGCCAC CTCAGTTGCT 
GATGAAGGCA TTGACATTGC ACAGTGCAAT CTTGTCATCC TTTATGAGTA TGTGGGCAAT 
GTCATCAAAA TGATCCAAAC CAGAGGCAGA GGAAGAGCAA GAGGTAGCAA GTGCTTCCTT 
CTGACTAGTA ATGCTGGTGT AATTGAAAAA GAACAAATAA ACATGTACAA AGAAAAAATG 
ATGAATGACT CTATTTTACG CCTTCAGACA TGGGACGAAG CAGTATTTAG GGAAAAGATT 
CTGCATATAC AGACTCATGA AAAATTCATC AGAGATAGTC AAGAAAAACC AAAACCTGTA 
CCTGATAAGG AAAATAAAAA ACTGCTCTGC AGAAAGTGCA AAGCCTTGGC ATGTTACACA 
GCTGACGTAA GAGTGATAGA GGAATGCCAT TACACTGTGC TTGGAGATGC TTTTAAGGAA 
TGCTTTGTGA GTAGACCACA TCCCAAGCCA AAGCAGTTTT CAAGTTTTGA AAAAAGAGCA 
AAGATATTCT GTGCCCGACA GAACTGCAGC CATGACTGGG GAATCCATGT GAAGTACAAG 
ACATTTGAGA TTCCAGTTAT AAAAATTGAA AGTTTTGTGG TGGAGGATAT TGCAACTGGA 
GTTCAGACAC TGTACTCGAA GTGGAAGGAC TTTCATTTTG AGAAGATACC ATTTGATCCA 
GCAGAAATGT CCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( NM_014314.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055129.2 Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.

Price & Availability↑

CloneID	OHu25414
Clone ID Related Accession (Same CDS sequence)	NM_014314.4 , NM_014314.3
Accession Version	NM_014314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF038963.1, DN998145.1, BC070029.1, AL161783.20 and AA862613.1. This sequence is a reference standard in the RefSeqGene project. On Oct 14, 2005 this sequence version replaced NM_014314.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF038963.1, SRR1660807.175842.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACCG AGCAGCGACG CAGCCTGCAA GCCTTCCAGG ATTATATCCG GAAGACCCTG 
GACCCTACCT ACATCCTGAG CTACATGGCC CCCTGGTTTA GGGAGGAAGA GGTGCAGTAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCACACTTTT TCTCAAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGTGGCTTTT TGGATGCCCT AGACCATGCA 
GGTTATTCTG GACTTTATGA AGCCATTGAA AGTTGGGATT TCAAAAAAAT TGAAAAGTTG 
GAGGAGTATA GATTACTTTT AAAACGTTTA CAACCAGAAT TTAAAACCAG AATTATCCCA 
ACCGATATCA TTTCTGATCT GTCTGAATGT TTAATTAATC AGGAATGTGA AGAAATTCTA 
CAGATTTGCT CTACTAAGGG GATGATGGCA GGTGCAGAGA AATTGGTGGA ATGCCTTCTC 
AGATCAGACA AGGAAAACTG GCCCAAAACT TTGAAACTTG CTTTGGAGAA AGAAAGGAAC 
AAGTTCAGTG AACTGTGGAT TGTAGAGAAA GGTATAAAAG ATGTTGAAAC AGAAGATCTT 
GAGGATAAGA TGGAAACTTC TGACATACAG ATTTTCTACC AAGAAGATCC AGAATGCCAG 
AATCTTAGTG AGAATTCATG TCCACCTTCA GAAGTGTCTG ATACAAACTT GTACAGCCCA 
TTTAAACCAA GAAATTACCA ATTAGAGCTT GCTTTGCCTG CTATGAAAGG AAAAAACACA 
ATAATATGTG CTCCTACAGG TTGTGGAAAA ACCTTTGTTT CACTGCTTAT ATGTGAACAT 
CATCTTAAAA AATTCCCACA AGGACAAAAG GGGAAAGTTG TCTTTTTTGC GAATCAGATC 
CCAGTGTATG AACAGCAGAA ATCTGTATTC TCAAAATACT TTGAAAGACA TGGGTATAGA 
GTTACAGGCA TTTCTGGAGC AACAGCTGAG AATGTCCCAG TGGAACAGAT TGTTGAGAAC 
AATGACATCA TCATTTTAAC TCCACAGATT CTTGTGAACA ACCTTAAAAA GGGAACGATT 
CCATCACTAT CCATCTTTAC TTTGATGATA TTTGATGAAT GCCACAACAC TAGTAAACAA 
CACCCGTACA ATATGATCAT GTTTAATTAT CTAGATCAGA AACTTGGAGG ATCTTCAGGC 
CCACTGCCCC AGGTCATTGG GCTGACTGCC TCGGTTGGTG TTGGGGATGC CAAAAACACA 
GATGAAGCCT TGGATTATAT CTGCAAGCTG TGTGCTTCTC TTGATGCGTC AGTGATAGCA 
ACAGTCAAAC ACAATCTGGA GGAACTGGAG CAAGTTGTTT ATAAGCCCCA GAAGTTTTTC 
AGGAAAGTGG AATCACGGAT TAGCGACAAA TTTAAATACA TCATAGCTCA GCTGATGAGG 
GACACAGAGA GTCTGGCAAA GAGAATCTGC AAAGACCTCG AAAACTTATC TCAAATTCAA 
AATAGGGAAT TTGGAACACA GAAATATGAA CAATGGATTG TTACAGTTCA GAAAGCATGC 
ATGGTGTTCC AGATGCCAGA CAAAGATGAA GAGAGCAGGA TTTGTAAAGC CCTGTTTTTA 
TACACTTCAC ATTTGCGGAA ATATAATGAT GCCCTCATTA TCAGTGAGCA TGCACGAATG 
AAAGATGCTC TGGATTACTT GAAAGACTTC TTCAGCAATG TCCGAGCAGC AGGATTCGAT 
GAGATTGAGC AAGATCTTAC TCAGAGATTT GAAGAAAAGC TGCAGGAACT AGAAAGTGTT 
TCCAGGGATC CCAGCAATGA GAATCCTAAA CTTGAAGACC TCTGCTTCAT CTTACAAGAA 
GAGTACCACT TAAACCCAGA GACAATAACA ATTCTCTTTG TGAAAACCAG AGCACTTGTG 
GACGCTTTAA AAAATTGGAT TGAAGGAAAT CCTAAACTCA GTTTTCTAAA ACCTGGCATA 
TTGACTGGAC GTGGCAAAAC AAATCAGAAC ACAGGAATGA CCCTCCCGGC ACAGAAGTGT 
ATATTGGATG CATTCAAAGC CAGTGGAGAT CACAATATTC TGATTGCCAC CTCAGTTGCT 
GATGAAGGCA TTGACATTGC ACAGTGCAAT CTTGTCATCC TTTATGAGTA TGTGGGCAAT 
GTCATCAAAA TGATCCAAAC CAGAGGCAGA GGAAGAGCAA GAGGTAGCAA GTGCTTCCTT 
CTGACTAGTA ATGCTGGTGT AATTGAAAAA GAACAAATAA ACATGTACAA AGAAAAAATG 
ATGAATGACT CTATTTTACG CCTTCAGACA TGGGACGAAG CAGTATTTAG GGAAAAGATT 
CTGCATATAC AGACTCATGA AAAATTCATC AGAGATAGTC AAGAAAAACC AAAACCTGTA 
CCTGATAAGG AAAATAAAAA ACTGCTCTGC AGAAAGTGCA AAGCCTTGGC ATGTTACACA 
GCTGACGTAA GAGTGATAGA GGAATGCCAT TACACTGTGC TTGGAGATGC TTTTAAGGAA 
TGCTTTGTGA GTAGACCACA TCCCAAGCCA AAGCAGTTTT CAAGTTTTGA AAAAAGAGCA 
AAGATATTCT GTGCCCGACA GAACTGCAGC CATGACTGGG GAATCCATGT GAAGTACAAG 
ACATTTGAGA TTCCAGTTAT AAAAATTGAA AGTTTTGTGG TGGAGGATAT TGCAACTGGA 
GTTCAGACAC TGTACTCGAA GTGGAAGGAC TTTCATTTTG AGAAGATACC ATTTGATCCA 
GCAGAAATGT CCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055129.2
CDS	159..2936
Translation	MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK

Target ORF information:

RefSeq Version	NM_014314.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD/H-box helicase 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014314.3

ORF Insert Sequence:

ATGACCACCG AGCAGCGACG CAGCCTGCAA GCCTTCCAGG ATTATATCCG GAAGACCCTG 
GACCCTACCT ACATCCTGAG CTACATGGCC CCCTGGTTTA GGGAGGAAGA GGTGCAGTAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCACACTTTT TCTCAAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGTGGCTTTT TGGATGCCCT AGACCATGCA 
GGTTATTCTG GACTTTATGA AGCCATTGAA AGTTGGGATT TCAAAAAAAT TGAAAAGTTG 
GAGGAGTATA GATTACTTTT AAAACGTTTA CAACCAGAAT TTAAAACCAG AATTATCCCA 
ACCGATATCA TTTCTGATCT GTCTGAATGT TTAATTAATC AGGAATGTGA AGAAATTCTA 
CAGATTTGCT CTACTAAGGG GATGATGGCA GGTGCAGAGA AATTGGTGGA ATGCCTTCTC 
AGATCAGACA AGGAAAACTG GCCCAAAACT TTGAAACTTG CTTTGGAGAA AGAAAGGAAC 
AAGTTCAGTG AACTGTGGAT TGTAGAGAAA GGTATAAAAG ATGTTGAAAC AGAAGATCTT 
GAGGATAAGA TGGAAACTTC TGACATACAG ATTTTCTACC AAGAAGATCC AGAATGCCAG 
AATCTTAGTG AGAATTCATG TCCACCTTCA GAAGTGTCTG ATACAAACTT GTACAGCCCA 
TTTAAACCAA GAAATTACCA ATTAGAGCTT GCTTTGCCTG CTATGAAAGG AAAAAACACA 
ATAATATGTG CTCCTACAGG TTGTGGAAAA ACCTTTGTTT CACTGCTTAT ATGTGAACAT 
CATCTTAAAA AATTCCCACA AGGACAAAAG GGGAAAGTTG TCTTTTTTGC GAATCAGATC 
CCAGTGTATG AACAGCAGAA ATCTGTATTC TCAAAATACT TTGAAAGACA TGGGTATAGA 
GTTACAGGCA TTTCTGGAGC AACAGCTGAG AATGTCCCAG TGGAACAGAT TGTTGAGAAC 
AATGACATCA TCATTTTAAC TCCACAGATT CTTGTGAACA ACCTTAAAAA GGGAACGATT 
CCATCACTAT CCATCTTTAC TTTGATGATA TTTGATGAAT GCCACAACAC TAGTAAACAA 
CACCCGTACA ATATGATCAT GTTTAATTAT CTAGATCAGA AACTTGGAGG ATCTTCAGGC 
CCACTGCCCC AGGTCATTGG GCTGACTGCC TCGGTTGGTG TTGGGGATGC CAAAAACACA 
GATGAAGCCT TGGATTATAT CTGCAAGCTG TGTGCTTCTC TTGATGCGTC AGTGATAGCA 
ACAGTCAAAC ACAATCTGGA GGAACTGGAG CAAGTTGTTT ATAAGCCCCA GAAGTTTTTC 
AGGAAAGTGG AATCACGGAT TAGCGACAAA TTTAAATACA TCATAGCTCA GCTGATGAGG 
GACACAGAGA GTCTGGCAAA GAGAATCTGC AAAGACCTCG AAAACTTATC TCAAATTCAA 
AATAGGGAAT TTGGAACACA GAAATATGAA CAATGGATTG TTACAGTTCA GAAAGCATGC 
ATGGTGTTCC AGATGCCAGA CAAAGATGAA GAGAGCAGGA TTTGTAAAGC CCTGTTTTTA 
TACACTTCAC ATTTGCGGAA ATATAATGAT GCCCTCATTA TCAGTGAGCA TGCACGAATG 
AAAGATGCTC TGGATTACTT GAAAGACTTC TTCAGCAATG TCCGAGCAGC AGGATTCGAT 
GAGATTGAGC AAGATCTTAC TCAGAGATTT GAAGAAAAGC TGCAGGAACT AGAAAGTGTT 
TCCAGGGATC CCAGCAATGA GAATCCTAAA CTTGAAGACC TCTGCTTCAT CTTACAAGAA 
GAGTACCACT TAAACCCAGA GACAATAACA ATTCTCTTTG TGAAAACCAG AGCACTTGTG 
GACGCTTTAA AAAATTGGAT TGAAGGAAAT CCTAAACTCA GTTTTCTAAA ACCTGGCATA 
TTGACTGGAC GTGGCAAAAC AAATCAGAAC ACAGGAATGA CCCTCCCGGC ACAGAAGTGT 
ATATTGGATG CATTCAAAGC CAGTGGAGAT CACAATATTC TGATTGCCAC CTCAGTTGCT 
GATGAAGGCA TTGACATTGC ACAGTGCAAT CTTGTCATCC TTTATGAGTA TGTGGGCAAT 
GTCATCAAAA TGATCCAAAC CAGAGGCAGA GGAAGAGCAA GAGGTAGCAA GTGCTTCCTT 
CTGACTAGTA ATGCTGGTGT AATTGAAAAA GAACAAATAA ACATGTACAA AGAAAAAATG 
ATGAATGACT CTATTTTACG CCTTCAGACA TGGGACGAAG CAGTATTTAG GGAAAAGATT 
CTGCATATAC AGACTCATGA AAAATTCATC AGAGATAGTC AAGAAAAACC AAAACCTGTA 
CCTGATAAGG AAAATAAAAA ACTGCTCTGC AGAAAGTGCA AAGCCTTGGC ATGTTACACA 
GCTGACGTAA GAGTGATAGA GGAATGCCAT TACACTGTGC TTGGAGATGC TTTTAAGGAA 
TGCTTTGTGA GTAGACCACA TCCCAAGCCA AAGCAGTTTT CAAGTTTTGA AAAAAGAGCA 
AAGATATTCT GTGCCCGACA GAACTGCAGC CATGACTGGG GAATCCATGT GAAGTACAAG 
ACATTTGAGA TTCCAGTTAT AAAAATTGAA AGTTTTGTGG TGGAGGATAT TGCAACTGGA 
GTTCAGACAC TGTACTCGAA GTGGAAGGAC TTTCATTTTG AGAAGATACC ATTTGATCCA 
GCAGAAATGT CCAAATGA