HAUS5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HAUS5
Entrez Gene ID	23354
Full Name	HAUS augmin like complex subunit 5
Synonyms	KIAA0841,dgt5
General protein information	Preferred Names HAUS augmin like complex subunit 5 Names HAUS augmin-like complex subunit 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	HAUS5 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010].
Disorder MIM:	613432

mRNA and Protein(s)

mRNA	Protein	Name
NM_015302.1	NP_056117.1	HAUS augmin-like complex subunit 5
XM_011526684.2	XP_011524986.1	HAUS augmin-like complex subunit 5 isoform X1
NM_015302.2	NP_056117.1	HAUS augmin-like complex subunit 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	HAUS5	NP_056117.1
Mus musculus (house mouse)	Haus5	NP_082275.1
Rattus norvegicus (Norway rat)	Haus5	XP_002728777.1
Xenopus tropicalis (tropical clawed frog)	LOC100145714	NP_001120560.1
Pan troglodytes (chimpanzee)	HAUS5	XP_003316331.1
Danio rerio (zebrafish)	haus5	NP_001007439.1
Canis lupus familiaris (dog)	HAUS5	XP_533691.2
Bos taurus (cattle)	HAUS5	NP_001095401.1

Gene Ontology
Bibliography

Related articles in PubMed

ZNF131 suppresses centrosome fragmentation in glioblastoma stem-like cells through regulation of HAUS5.
Ding Y, Herman JA, Toledo CM, Lang JM, Corrin P, Girard EJ, Basom R, Delrow JJ, Olson JM, Paddison PJ
Oncotarget8(30)48545-48562(2017 Jul)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, H?rich? JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM
Science (New York, N.Y.)328(5978)593-9(2010 Apr)

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L
Current biology : CB19(10)816-26(2009 May)

The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.
Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G
Proceedings of the National Academy of Sciences of the United States of America106(17)6998-7003(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HAUS5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAUS5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06927	NM_015302.2 Latest version!	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu66048	XM_011526684.2 Latest version!	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OHu06927	NM_015302.1	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAUS5 ( NM_015302.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_056117.1 Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.

Price & Availability↑

CloneID	OHu06927
Clone ID Related Accession (Same CDS sequence)	NM_015302.1 , NM_015302.2
Accession Version	NM_015302.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	HAUS augmin-like complex subunit 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC064390.1 and AB020648.1. On or before Jun 29, 2007 this sequence version replaced XM_932194.2, XM_049237.8, XM_932173.2, XM_942304.2, XM_945898.2, XM_945890.2. ##Evidence-Data-START## Transcript exon combination :: AB020648.1, SRR1660809.133429.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTAG CGCAGGAAGC GCGGGAACTG GGTTGCTGGG CGGTCGAAGA GATGGGGGTG 
CCCGTGGCGG CCCGGGCCCC GGAATCGACG CTGCGCAGGC TGTGTCTGGG CCAGGGGGCT 
GACATCTGGG CCTACATCTT GCAGCATGTG CACAGTCAGA GGACTGTCAA GAAGATCCGG 
GGAAACCTAC TCTGGTATGG CCACCAGGAC AGTCCACAGG TCCGTCGGAA GTTAGAGCTG 
GAAGCTGCTG TGACCCGCCT GCGGGCAGAA ATCCAGGAAC TCGACCAGAG CCTGGAGCTG 
ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056117.1
CDS	52..1953
Translation	MELAQEARELGCWAVEEMGVPVAARAPESTLRRLCLGQGADIWAYILQHVHSQRTVKKIRGNLLWYGHQDSPQVRRKLELEAAVTRLRAEIQELDQSLELMERDTEAQDTAMEQARQHTQDTQRRALLLRAQAGAMRRQQHTLRDPMQRLQNQLRRLQDMERKAKVDVTFGSLTSAALGLEPVVLRDVRTACTLRAQFLQNLLLPQAKRGSLPTPHDDHFGTSYQQWLSSVETLLTNHPPGHVLAALEHLAAEREAEIRSLCSGDGLGDTEISRPQAPDQSDSSQTLPSMVHLIQEGWRTVGVLVSQRSTLLKERQVLTQRLQGLVEEVERRVLGSSERQVLILGLRRCCLWTELKALHDQSQELQDAAGHRQLLLRELQAKQQRILHWRQLVEETQEQVRLLIKGNSASKTRLCRSPGEVLALVQRKVVPTFEAVAPQSRELLRCLEEEVRHLPHILLGTLLRHRPGELKPLPTVLPSIHQLHPASPRGSSFIALSHKLGLPPGKASELLLPAAASLRQDLLLLQDQRSLWCWDLLHMKTSLPPGLPTQELLQIQASQEKQQKENLGQALKRLEKLLKQALERIPELQGIVGDWWEQPGQAALSEELCQGLSLPQWRLRWVQAQGALQKLCS

Target ORF information:

RefSeq Version	NM_015302.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015302.1

ORF Insert Sequence:

ATGGAGCTAG CGCAGGAAGC GCGGGAACTG GGTTGCTGGG CGGTCGAAGA GATGGGGGTG 
CCCGTGGCGG CCCGGGCCCC GGAATCGACG CTGCGCAGGC TGTGTCTGGG CCAGGGGGCT 
GACATCTGGG CCTACATCTT GCAGCATGTG CACAGTCAGA GGACTGTCAA GAAGATCCGG 
GGAAACCTAC TCTGGTATGG CCACCAGGAC AGTCCACAGG TCCGTCGGAA GTTAGAGCTG 
GAAGCTGCTG TGACCCGCCT GCGGGCAGAA ATCCAGGAAC TCGACCAGAG CCTGGAGCTG 
ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HAUS5 ( NM_015302.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056117.1 Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.

Price & Availability↑

CloneID	OHu06927
Clone ID Related Accession (Same CDS sequence)	NM_015302.1 , NM_015302.2
Accession Version	NM_015302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	HAUS augmin-like complex subunit 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC002115.1, BC064390.1 and AB020648.1. On Nov 23, 2018 this sequence version replaced NM_015302.1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.78718.1, AB020648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000203166.10/ ENSP00000439056.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTAG CGCAGGAAGC GCGGGAACTG GGTTGCTGGG CGGTCGAAGA GATGGGGGTG 
CCCGTGGCGG CCCGGGCCCC GGAATCGACG CTGCGCAGGC TGTGTCTGGG CCAGGGGGCT 
GACATCTGGG CCTACATCTT GCAGCATGTG CACAGTCAGA GGACTGTCAA GAAGATCCGG 
GGAAACCTAC TCTGGTATGG CCACCAGGAC AGTCCACAGG TCCGTCGGAA GTTAGAGCTG 
GAAGCTGCTG TGACCCGCCT GCGGGCAGAA ATCCAGGAAC TCGACCAGAG CCTGGAGCTG 
ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056117.1
CDS	61..1962
Translation	MELAQEARELGCWAVEEMGVPVAARAPESTLRRLCLGQGADIWAYILQHVHSQRTVKKIRGNLLWYGHQDSPQVRRKLELEAAVTRLRAEIQELDQSLELMERDTEAQDTAMEQARQHTQDTQRRALLLRAQAGAMRRQQHTLRDPMQRLQNQLRRLQDMERKAKVDVTFGSLTSAALGLEPVVLRDVRTACTLRAQFLQNLLLPQAKRGSLPTPHDDHFGTSYQQWLSSVETLLTNHPPGHVLAALEHLAAEREAEIRSLCSGDGLGDTEISRPQAPDQSDSSQTLPSMVHLIQEGWRTVGVLVSQRSTLLKERQVLTQRLQGLVEEVERRVLGSSERQVLILGLRRCCLWTELKALHDQSQELQDAAGHRQLLLRELQAKQQRILHWRQLVEETQEQVRLLIKGNSASKTRLCRSPGEVLALVQRKVVPTFEAVAPQSRELLRCLEEEVRHLPHILLGTLLRHRPGELKPLPTVLPSIHQLHPASPRGSSFIALSHKLGLPPGKASELLLPAAASLRQDLLLLQDQRSLWCWDLLHMKTSLPPGLPTQELLQIQASQEKQQKENLGQALKRLEKLLKQALERIPELQGIVGDWWEQPGQAALSEELCQGLSLPQWRLRWVQAQGALQKLCS

Target ORF information:

RefSeq Version	NM_015302.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015302.2

ORF Insert Sequence:

ATGGAGCTAG CGCAGGAAGC GCGGGAACTG GGTTGCTGGG CGGTCGAAGA GATGGGGGTG 
CCCGTGGCGG CCCGGGCCCC GGAATCGACG CTGCGCAGGC TGTGTCTGGG CCAGGGGGCT 
GACATCTGGG CCTACATCTT GCAGCATGTG CACAGTCAGA GGACTGTCAA GAAGATCCGG 
GGAAACCTAC TCTGGTATGG CCACCAGGAC AGTCCACAGG TCCGTCGGAA GTTAGAGCTG 
GAAGCTGCTG TGACCCGCCT GCGGGCAGAA ATCCAGGAAC TCGACCAGAG CCTGGAGCTG 
ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HAUS5 ( XM_011526684.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524986.1 Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66048
Clone ID Related Accession (Same CDS sequence)	XM_011526684.2
Accession Version	XM_011526684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	HAUS augmin-like complex subunit 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524986.1
CDS	239..1840
Translation	MERDTEAQDTAMEQARQHTQDTQRRALLLRAQAGAMRRQQHTLRDPMQRLQNQLRRLQDMERKAKVDVTFGSLTSAALGLEPVVLRDVRTACTLRAQFLQNLLLPQAKRGSLPTPHDDHFGTSYQQWLSSVETLLTNHPPGHVLAALEHLAAEREAEIRSLCSGDGLGDTEISRPQAPDQSDSSQTLPSMVHLIQEGWRTVGVLVSQRSTLLKERQVLTQRLQGLVEEVERRVLGSSERQVLILGLRRCCLWTELKALHDQSQELQDAAGHRQLLLRELQAKQQRILHWRQLVEETQEQVRLLIKGNSASKTRLCRSPGEVLALVQRKVVPTFEAVAPQSRELLRCLEEEVRHLPHILLGTLLRHRPGELKPLPTVLPSIHQLHPASPRGSSFIALSHKLGLPPGKASELLLPAAASLRQDLLLLQDQRSLWCWDLLHMKTSLPPGLPTQELLQIQASQEKQQKENLGQALKRLEKLLKQALERIPELQGIVGDWWEQPGQAALSEELCQGLSLPQWRLRWVQAQGALQKLCS

Target ORF information:

RefSeq Version	XM_011526684.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HAUS augmin like complex subunit 5 (HAUS5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526684.2

ORF Insert Sequence:

ATGGAGCGAG ACACTGAGGC TCAGGACACG GCCATGGAGC AGGCACGTCA GCACACTCAA 
GACACCCAGC GTCGAGCTCT CCTCCTCCGG GCCCAAGCTG GGGCCATGCG AAGACAGCAG 
CATACGCTCC GAGATCCCAT GCAGCGGCTG CAGAATCAAC TGAGGCGCCT GCAGGACATG 
GAGAGGAAAG CCAAAGTAGA TGTGACCTTT GGATCCCTCA CGTCGGCAGC TCTGGGCCTG 
GAGCCCGTGG TCCTGCGTGA TGTCCGAACA GCCTGCACCC TCCGGGCCCA GTTCCTGCAG 
AACCTCCTGC TTCCCCAGGC CAAGAGGGGC AGCCTCCCAA CCCCTCATGA TGACCACTTT 
GGCACTTCGT ACCAGCAGTG GCTGAGCTCA GTGGAGACGC TGCTGACAAA CCACCCCCCA 
GGCCACGTCC TGGCTGCCTT GGAGCACCTG GCTGCAGAGC GGGAGGCAGA GATTCGGTCC 
CTGTGCAGTG GGGATGGGCT TGGCGACACA GAGATATCCA GACCCCAGGC CCCGGACCAG 
TCAGACTCCA GCCAGACCCT GCCGTCCATG GTTCATCTCA TCCAGGAGGG CTGGCGGACT 
GTGGGTGTGC TGGTCTCCCA GCGGAGCACC CTCCTGAAGG AGCGGCAAGT CTTGACCCAG 
CGCCTCCAGG GCCTGGTGGA GGAGGTGGAG AGACGCGTCC TGGGATCCAG TGAGAGGCAG 
GTGCTGATAC TGGGGCTTCG GCGCTGTTGC CTGTGGACGG AGCTCAAGGC CCTGCACGAT 
CAGAGCCAGG AGCTGCAGGA TGCAGCTGGG CATCGGCAGC TCCTGCTGAG GGAGCTACAG 
GCCAAACAGC AGCGGATCCT GCACTGGCGC CAGCTGGTGG AGGAGACCCA GGAACAGGTC 
CGCCTGCTCA TCAAGGGAAA CTCGGCCAGC AAGACCCGCC TGTGCCGGAG CCCGGGGGAG 
GTGCTAGCTC TGGTCCAGCG AAAGGTGGTC CCTACATTTG AGGCAGTGGC ACCACAGAGC 
CGGGAGCTGC TGCGCTGTCT GGAGGAGGAA GTCCGGCATT TGCCCCACAT TCTGTTGGGC 
ACGCTGCTGC GGCACAGGCC GGGAGAGTTG AAGCCCCTGC CCACGGTCCT CCCATCCATC 
CACCAGCTGC ACCCCGCGTC CCCAAGGGGC TCCAGCTTCA TAGCGCTGAG CCACAAGCTG 
GGGCTGCCTC CAGGGAAGGC CTCGGAGCTG CTCCTGCCGG CGGCTGCCTC TCTTCGCCAG 
GACCTTCTGC TCCTGCAGGA CCAGCGGAGC CTCTGGTGCT GGGATCTACT CCACATGAAG 
ACCAGCCTGC CGCCAGGCCT TCCCACCCAG GAGCTGCTGC AGATCCAGGC ATCCCAGGAA 
AAACAGCAGA AAGAGAACCT GGGGCAGGCT CTGAAGAGGC TGGAGAAGCT ACTGAAACAG 
GCACTGGAGC GAATCCCTGA GCTGCAGGGG ATCGTGGGGG ACTGGTGGGA GCAGCCAGGC 
CAGGCCGCCC TCTCTGAGGA GCTCTGCCAG GGCCTGTCCC TGCCCCAGTG GCGGCTGCGC 
TGGGTTCAGG CCCAGGGGGC CCTGCAGAAG CTGTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZNF131 suppresses centrosome fragmentation in glioblastoma stem-like cells through regulation of HAUS5.
Oncotarget8(30)48545-48562(2017 Jul)
Ding Y,Herman JA,Toledo CM,Lang JM,Corrin P,Girard EJ,Basom R,Delrow JJ,Olson JM,Paddison PJ

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science (New York, N.Y.)328(5978)593-9(2010 Apr)
Hutchins JR,Toyoda Y,Hegemann B,Poser I,H?rich? JK,Sykora MM,Augsburg M,Hudecz O,Buschhorn BA,Bulkescher J,Conrad C,Comartin D,Schleiffer A,Sarov M,Pozniakovsky A,Slabicki MM,Schloissnig S,Steinmacher I,Leuschner M,Ssykor A,Lawo S,Pelletier L,Stark H,Nasmyth K,Ellenberg J,Durbin R,Buchholz F,Mechtler K,Hyman AA,Peters JM

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Current biology : CB19(10)816-26(2009 May)
Lawo S,Bashkurov M,Mullin M,Ferreria MG,Kittler R,Habermann B,Tagliaferro A,Poser I,Hutchins JR,Hegemann B,Pinchev D,Buchholz F,Peters JM,Hyman AA,Gingras AC,Pelletier L

The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.
Proceedings of the National Academy of Sciences of the United States of America106(17)6998-7003(2009 Apr)
Uehara R,Nozawa RS,Tomioka A,Petry S,Vale RD,Obuse C,Goshima G

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HAUS5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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