MCF2L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCF2L
Entrez Gene ID	23263
Full Name	MCF.2 cell line derived transforming sequence like
Synonyms	ARHGEF14,DBS,OST
General protein information	Preferred Names MCF.2 cell line derived transforming sequence like Names guanine nucleotide exchange factor DBS DBL's big sister MCF2 transforming sequence-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q34
Summary	This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016].
Disorder MIM:	609499

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320815.1	NP_001307744.1	guanine nucleotide exchange factor DBS isoform c precursor
NM_001366645.1	NP_001353574.1	guanine nucleotide exchange factor DBS isoform g
XM_017020495.1	XP_016875984.1	guanine nucleotide exchange factor DBS isoform X6
XM_011537491.1	XP_011535793.1	guanine nucleotide exchange factor DBS isoform X11
NM_001320815.2	NP_001307744.1	guanine nucleotide exchange factor DBS isoform c precursor
NM_001320816.1	NP_001307745.1	guanine nucleotide exchange factor DBS isoform d
XM_017020499.1	XP_016875988.1	guanine nucleotide exchange factor DBS isoform X15
NM_001366646.1	NP_001353575.1	guanine nucleotide exchange factor DBS isoform h
NM_001320817.2	NP_001307746.1	guanine nucleotide exchange factor DBS isoform e precursor
XM_011537482.1	XP_011535784.1	guanine nucleotide exchange factor DBS isoform X2
XM_011537483.2	XP_011535785.1	guanine nucleotide exchange factor DBS isoform X2
XM_011537487.1	XP_011535789.1	guanine nucleotide exchange factor DBS isoform X8
XM_017020496.1	XP_016875985.1	guanine nucleotide exchange factor DBS isoform X12
XM_017020498.1	XP_016875987.1	guanine nucleotide exchange factor DBS isoform X14
NM_001112732.3	NP_001106203.2	guanine nucleotide exchange factor DBS isoform a
XM_017020494.1	XP_016875983.1	guanine nucleotide exchange factor DBS isoform X4
XM_011537490.1	XP_011535792.1	guanine nucleotide exchange factor DBS isoform X10
NM_024979.4	NP_079255.4	guanine nucleotide exchange factor DBS isoform b precursor
XM_017020497.1	XP_016875986.1	guanine nucleotide exchange factor DBS isoform X13
NM_001366644.2	NP_001353573.1	guanine nucleotide exchange factor DBS isoform f
XM_011537486.2	XP_011535788.2	guanine nucleotide exchange factor DBS isoform X1
XM_017020493.1	XP_016875982.1	guanine nucleotide exchange factor DBS isoform X3
XM_011537484.1	XP_011535786.1	guanine nucleotide exchange factor DBS isoform X5
XM_011537485.1	XP_011535787.1	guanine nucleotide exchange factor DBS isoform X7
XM_011537488.1	XP_011535790.1	guanine nucleotide exchange factor DBS isoform X9
XM_017020499.2	XP_016875988.1	guanine nucleotide exchange factor DBS isoform X15
NM_001366644.1	NP_001353573.1	guanine nucleotide exchange factor DBS isoform f
NM_001320817.1	NP_001307746.1	guanine nucleotide exchange factor DBS isoform e precursor
NM_001112732.2	NP_001106203.2	guanine nucleotide exchange factor DBS isoform a
NM_001366646.2	NP_001353575.1	guanine nucleotide exchange factor DBS isoform h
NM_001320816.2	NP_001307745.1	guanine nucleotide exchange factor DBS isoform d
NM_001366645.2	NP_001353574.1	guanine nucleotide exchange factor DBS isoform g

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497730	XP_002933052.2
Homo sapiens (human)	MCF2L	NP_001106203.2
Pan troglodytes (chimpanzee)	MCF2L	XP_003314263.1
	MCF2L	XP_001103476.2
Mus musculus (house mouse)	Mcf2l	NP_835177.2
Rattus norvegicus (Norway rat)	Mcf2l	NP_446403.2
Canis lupus familiaris (dog)	MCF2L	XP_005634173.1
Gallus gallus (chicken)	MCF2L	NP_001238992.1
Danio rerio (zebrafish)	mcf2la	XP_005171065.1
Bos taurus (cattle)	MCF2L	XP_005214085.1

Gene Ontology
Bibliography

Related articles in PubMed

Establishment of a human primary pancreatic cancer mouse model to examine and investigate gemcitabine resistance.
Zhang YJ, Wen CL, Qin YX, Tang XM, Shi MM, Shen BY, Fang Y
Oncology reports38(6)3335-3346(2017 Dec)

A rare variant in MCF2L identified using exclusion linkage in a pedigree with premature atherosclerosis.
Maiwald S, Motazacker MM, van Capelleveen JC, Sivapalaratnam S, van der Wal AC, van der Loos C, Kastelein JJ, Ouwehand WH, Hovingh GK, Trip MD, van Buul JD, Dallinga-Thie GM
European journal of human genetics : EJHG24(1)86-91(2016 Jan)

Expression analysis of the osteoarthritis genetic susceptibility locus mapping to an intron of the MCF2L gene and marked by the polymorphism rs11842874.
Shepherd C, Skelton AJ, Rushton MD, Reynard LN, Loughlin J
BMC medical genetics16108(2015 Nov)

Regulation of vesicle transport and cell motility by Golgi-localized Dbs.
Fitzpatrick ER, Hu T, Ciccarelli BT, Whitehead IP
Small GTPases5(4)1-12(2014)

Genetic contribution to radiographic severity in osteoarthritis of the knee.
Valdes AM, Doherty S, Muir KR, Zhang W, Maciewicz RA, Wheeler M, Arden N, Cooper C, Doherty M
Annals of the rheumatic diseases71(9)1537-40(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A rare variant in MCF2L has been identified using exclusion linkage in a pedigree with premature atherosclerosis.
Title: A rare variant in MCF2L identified using exclusion linkage in a pedigree with premature atherosclerosis.

MCF2L is subject to a cis-acting eQTL in synovial membrane that correlates with the OA association signal. This signal contains several functional SNPs that could account for the susceptibility and which therefore merit further investigation
Title: Expression analysis of the osteoarthritis genetic susceptibility locus mapping to an intron of the MCF2L gene and marked by the polymorphism rs11842874.

In conclusion, we find that GDF5, but not COG5 or MCF2L, influence the extent of radiographic damage in knee osteoarthritis
Title: Genetic contribution to radiographic severity in osteoarthritis of the knee.

A single nucleotide polymorphism in MCF2L is associated with osteoarthritis.
Title: A variant in MCF2L is associated with osteoarthritis.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following MCF2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu47429	NM_001320817.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu84481	NM_001366644.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu84479	XM_017020495.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu84481	XM_017020497.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu101638	NM_001320816.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu101981	NM_001320815.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84480	NM_001366645.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84482	NM_001366646.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84476	XM_011537486.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu84477	XM_017020493.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu84478	XM_017020494.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu84480	XM_017020496.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84482	XM_017020498.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84483	XM_017020499.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu22048	NM_024979.4 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu22268	NM_001112732.3 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65832	XM_011537482.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65832	XM_011537483.2 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65833	XM_011537484.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65834	XM_011537485.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65836	XM_011537487.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65837	XM_011537488.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65839	XM_011537490.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65840	XM_011537491.1 Latest version!	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu101981	NM_001320815.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84483	XM_017020499.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84481	NM_001366644.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu47429	NM_001320817.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu22268	NM_001112732.2	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu84482	NM_001366646.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu101638	NM_001320816.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu84480	NM_001366645.1	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCF2L ( NM_001320817.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307746.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu47429
Clone ID Related Accession (Same CDS sequence)	NM_001320817.2 , NM_001320817.1
Accession Version	NM_001320817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476653.1, AK295047.1 and BC020208.1. On May 31, 2019 this sequence version replaced NM_001320817.1. Transcript Variant: This variant (5) contains differences in the both UTRs and both the 5' and 3' coding regions, compared to variant 1. The resulting isoform (e) has distinct N- and C-termini and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011853.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END##

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307746.1
CDS	198..3152
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKEP

Target ORF information:

RefSeq Version	NM_001320817.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320817.2

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001320817.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307746.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.

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CloneID	OHu47429
Clone ID Related Accession (Same CDS sequence)	NM_001320817.2 , NM_001320817.1
Accession Version	NM_001320817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-22
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476653.1, AK295047.1 and BC020208.1. On Mar 5, 2016 this sequence version replaced XM_005268316.1. Transcript Variant: This variant (5) contains differences in the both UTRs and both the 5' and 3' coding regions, compared to variant 1. The resulting isoform (e) has distinct N- and C-termini and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011853.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END##

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307746.1
CDS	198..3152
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKEP

Target ORF information:

RefSeq Version	NM_001320817.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320817.1

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366644.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353573.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu84481
Clone ID Related Accession (Same CDS sequence)	XM_017020497.1 , NM_001366644.2 , NM_001366644.1
Accession Version	NM_001366644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356740.19, AL596093.18, AL162454.23 and AL137002.19. On May 31, 2019 this sequence version replaced NM_001366644.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.216612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END##

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353573.1
CDS	650..3940
Translation	MHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366644.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366644.2

ORF Insert Sequence:

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366644.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353573.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.

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CloneID	OHu84481
Clone ID Related Accession (Same CDS sequence)	XM_017020497.1 , NM_001366644.2 , NM_001366644.1
Accession Version	NM_001366644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356740.19, AL596093.18, AL162454.23 and AL137002.19. On Oct 13, 2018 this sequence version replaced XM_017020497.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.216612.1 [ECO:0000332] ##Evidence-Data-END##

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353573.1
CDS	650..3940
Translation	MHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366644.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366644.1

ORF Insert Sequence:

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020495.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875984.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

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CloneID	OHu84479
Clone ID Related Accession (Same CDS sequence)	XM_017020495.1
Accession Version	XM_017020495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGGTTGGA GCAAAACGTC CCACTCACTG GAGGCACCTG AGGACGACGG GGGCTGGTCA 
AGTGCAGAGG AGCAGATTAA CTCGTCCGAC GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG 
AAGCTGGTTC CAGGTAAATA CACGGTCGTG GCGGACCACG AGAAGGGAGG CCCCGATGCG 
CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG CTGGTGCAGG AGGGCGACGA GGGCCTCTGG 
TACGTCAGGG ACCCGACCAC TGGCAAGGAG GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC 
CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG 
GCCGTGCTGA GCAACTCGTC CAGCTGCAGC GAGGGCGGCC AGGCCCCCTT CTCCGACCTG 
CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875984.1
CDS	113..3661
Translation	MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCCGLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020495.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020495.1

ORF Insert Sequence:

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGGTTGGA GCAAAACGTC CCACTCACTG GAGGCACCTG AGGACGACGG GGGCTGGTCA 
AGTGCAGAGG AGCAGATTAA CTCGTCCGAC GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG 
AAGCTGGTTC CAGGTAAATA CACGGTCGTG GCGGACCACG AGAAGGGAGG CCCCGATGCG 
CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG CTGGTGCAGG AGGGCGACGA GGGCCTCTGG 
TACGTCAGGG ACCCGACCAC TGGCAAGGAG GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC 
CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG 
GCCGTGCTGA GCAACTCGTC CAGCTGCAGC GAGGGCGGCC AGGCCCCCTT CTCCGACCTG 
CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020497.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875986.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.

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CloneID	OHu84481
Clone ID Related Accession (Same CDS sequence)	XM_017020497.1 , NM_001366644.2 , NM_001366644.1
Accession Version	XM_017020497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875986.1
CDS	649..3939
Translation	MHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020497.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020497.1

ORF Insert Sequence:

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGGTTG GAGCAAAACG 
TCCCACTCAC TGGAGGCACC TGAGGACGAC GGGGGCTGGT CAAGTGCAGA GGAGCAGATT 
AACTCGTCCG ACGCAGAGGA GGACGGCGGG TTGGGCCCCA AGAAGCTGGT TCCAGGTAAA 
TACACGGTCG TGGCGGACCA CGAGAAGGGA GGCCCCGATG CGCTGCGCGT GAGGAGCGGG 
GACGTGGTGG AGCTGGTGCA GGAGGGCGAC GAGGGCCTCT GGTACGTCAG GGACCCGACC 
ACTGGCAAGG AGGGCTGGGT GCCGGCCAGC AGCCTGTCCG TCCGGCTCGG CCCGTCCGGC 
TCGGCCCAGT GCCTGAGCAG CTCAGAGTCG AGCCCGGGGT CGGCCGTGCT GAGCAACTCG 
TCCAGCTGCA GCGAGGGCGG CCAGGCCCCC TTCTCCGACC TGCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001320816.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307745.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.

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CloneID	OHu101638
Clone ID Related Accession (Same CDS sequence)	NM_001320816.1 , NM_001320816.2
Accession Version	NM_001320816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-22
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356740.19, DB276568.1, AB116074.1, AL137002.19, AK295047.1 and AW241629.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in an isoform (d) with a distinct and shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGGTTGG AGCAAAACGT CCCACTCACT GGAGGCACCT 
GAGGACGACG GGGGCTGGTC AAGTGCAGAG GAGCAGATTA ACTCGTCCGA CGCAGAGGAG 
GACGGCGGGT TGGGCCCCAA GAAGCTGGTT CCAGGTAAAT ACACGGTCGT GGCGGACCAC 
GAGAAGGGAG GCCCCGATGC GCTGCGCGTG AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG 
GAGGGCGACG AGGGCCTCTG GTACGTCAGG GACCCGACCA CTGGCAAGGA GGGCTGGGTG 
CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC 
TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG AGCAACTCGT CCAGCTGCAG CGAGGGCGGC 
CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307745.1
CDS	357..3746
Translation	MAEKGASRGTLRRLWSLPRRRRGTAGRSRPDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001320816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320816.1

ORF Insert Sequence:

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGGTTGG AGCAAAACGT CCCACTCACT GGAGGCACCT 
GAGGACGACG GGGGCTGGTC AAGTGCAGAG GAGCAGATTA ACTCGTCCGA CGCAGAGGAG 
GACGGCGGGT TGGGCCCCAA GAAGCTGGTT CCAGGTAAAT ACACGGTCGT GGCGGACCAC 
GAGAAGGGAG GCCCCGATGC GCTGCGCGTG AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG 
GAGGGCGACG AGGGCCTCTG GTACGTCAGG GACCCGACCA CTGGCAAGGA GGGCTGGGTG 
CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC 
TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG AGCAACTCGT CCAGCTGCAG CGAGGGCGGC 
CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001320816.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307745.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101638
Clone ID Related Accession (Same CDS sequence)	NM_001320816.1 , NM_001320816.2
Accession Version	NM_001320816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB276568.1, AB116074.1, AL137002.19, AK295047.1 and AW241629.1. On Jun 1, 2019 this sequence version replaced NM_001320816.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in an isoform (d) with a distinct and shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGGTTGG AGCAAAACGT CCCACTCACT GGAGGCACCT 
GAGGACGACG GGGGCTGGTC AAGTGCAGAG GAGCAGATTA ACTCGTCCGA CGCAGAGGAG 
GACGGCGGGT TGGGCCCCAA GAAGCTGGTT CCAGGTAAAT ACACGGTCGT GGCGGACCAC 
GAGAAGGGAG GCCCCGATGC GCTGCGCGTG AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG 
GAGGGCGACG AGGGCCTCTG GTACGTCAGG GACCCGACCA CTGGCAAGGA GGGCTGGGTG 
CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC 
TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG AGCAACTCGT CCAGCTGCAG CGAGGGCGGC 
CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307745.1
CDS	303..3692
Translation	MAEKGASRGTLRRLWSLPRRRRGTAGRSRPDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001320816.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320816.2

ORF Insert Sequence:

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGGTTGG AGCAAAACGT CCCACTCACT GGAGGCACCT 
GAGGACGACG GGGGCTGGTC AAGTGCAGAG GAGCAGATTA ACTCGTCCGA CGCAGAGGAG 
GACGGCGGGT TGGGCCCCAA GAAGCTGGTT CCAGGTAAAT ACACGGTCGT GGCGGACCAC 
GAGAAGGGAG GCCCCGATGC GCTGCGCGTG AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG 
GAGGGCGACG AGGGCCTCTG GTACGTCAGG GACCCGACCA CTGGCAAGGA GGGCTGGGTG 
CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC 
TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG AGCAACTCGT CCAGCTGCAG CGAGGGCGGC 
CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001320815.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307744.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.

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CloneID	OHu101981
Clone ID Related Accession (Same CDS sequence)	NM_001320815.1 , NM_001320815.2
Accession Version	NM_001320815.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476653.1, AK295047.1, AL162454.23, AL137002.19, DA348090.1 and AW241629.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (c) has a distinct N-terminus and is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.98250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGCTT CTCCTACCAG TCCTGACAAA AAAGCCAAGC GGCATGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTGTGCG AGGTTGGAGC AAAACGTCCC ACTCACTGGA GGCACCTGAG 
GACGACGGGG GCTGGTCAAG TGCAGAGGAG CAGATTAACT CGTCCGACGC AGAGGAGGAC 
GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA GGTAAATACA CGGTCGTGGC GGACCACGAG 
AAGGGAGGCC CCGATGCGCT GCGCGTGAGG AGCGGGGACG TGGTGGAGCT GGTGCAGGAG 
GGCGACGAGG GCCTCTGGTA CGTCAGGGAC CCGACCACTG GCAAGGAGGG CTGGGTGCCG 
GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA 
GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC AACTCGTCCA GCTGCAGCGA GGGCGGCCAG 
GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307744.1
CDS	198..3644
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001320815.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320815.1

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGCTT CTCCTACCAG TCCTGACAAA AAAGCCAAGC GGCATGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTGTGCG AGGTTGGAGC AAAACGTCCC ACTCACTGGA GGCACCTGAG 
GACGACGGGG GCTGGTCAAG TGCAGAGGAG CAGATTAACT CGTCCGACGC AGAGGAGGAC 
GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA GGTAAATACA CGGTCGTGGC GGACCACGAG 
AAGGGAGGCC CCGATGCGCT GCGCGTGAGG AGCGGGGACG TGGTGGAGCT GGTGCAGGAG 
GGCGACGAGG GCCTCTGGTA CGTCAGGGAC CCGACCACTG GCAAGGAGGG CTGGGTGCCG 
GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA 
GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC AACTCGTCCA GCTGCAGCGA GGGCGGCCAG 
GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001320815.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307744.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.

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CloneID	OHu101981
Clone ID Related Accession (Same CDS sequence)	NM_001320815.1 , NM_001320815.2
Accession Version	NM_001320815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476653.1, AK295047.1, AL162454.23, AL137002.19, DA348090.1 and AW241629.1. On May 31, 2019 this sequence version replaced NM_001320815.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (c) has a distinct N-terminus and is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.98250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGCTT CTCCTACCAG TCCTGACAAA AAAGCCAAGC GGCATGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTGTGCG AGGTTGGAGC AAAACGTCCC ACTCACTGGA GGCACCTGAG 
GACGACGGGG GCTGGTCAAG TGCAGAGGAG CAGATTAACT CGTCCGACGC AGAGGAGGAC 
GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA GGTAAATACA CGGTCGTGGC GGACCACGAG 
AAGGGAGGCC CCGATGCGCT GCGCGTGAGG AGCGGGGACG TGGTGGAGCT GGTGCAGGAG 
GGCGACGAGG GCCTCTGGTA CGTCAGGGAC CCGACCACTG GCAAGGAGGG CTGGGTGCCG 
GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA 
GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC AACTCGTCCA GCTGCAGCGA GGGCGGCCAG 
GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307744.1
CDS	198..3644
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001320815.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320815.2

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGCTT CTCCTACCAG TCCTGACAAA AAAGCCAAGC GGCATGAAGT AAAGAGCGAC 
CCGACTCCCT TTGGTGTGCG AGGTTGGAGC AAAACGTCCC ACTCACTGGA GGCACCTGAG 
GACGACGGGG GCTGGTCAAG TGCAGAGGAG CAGATTAACT CGTCCGACGC AGAGGAGGAC 
GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA GGTAAATACA CGGTCGTGGC GGACCACGAG 
AAGGGAGGCC CCGATGCGCT GCGCGTGAGG AGCGGGGACG TGGTGGAGCT GGTGCAGGAG 
GGCGACGAGG GCCTCTGGTA CGTCAGGGAC CCGACCACTG GCAAGGAGGG CTGGGTGCCG 
GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA 
GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC AACTCGTCCA GCTGCAGCGA GGGCGGCCAG 
GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366645.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353574.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.

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CloneID	OHu84480
Clone ID Related Accession (Same CDS sequence)	NM_001366645.1 , XM_017020496.1 , NM_001366645.2
Accession Version	NM_001366645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596093.18, AL162454.23 and AL137002.19. On Oct 13, 2018 this sequence version replaced XM_017020496.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.271873.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353574.1
CDS	80..3418
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366645.1

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366645.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353574.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.

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CloneID	OHu84480
Clone ID Related Accession (Same CDS sequence)	NM_001366645.1 , XM_017020496.1 , NM_001366645.2
Accession Version	NM_001366645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596093.18, AL162454.23 and AL137002.19. On May 31, 2019 this sequence version replaced NM_001366645.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.271873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353574.1
CDS	80..3418
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366645.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366645.2

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366646.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353575.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu84482
Clone ID Related Accession (Same CDS sequence)	NM_001366646.1 , XM_017020498.1 , NM_001366646.2
Accession Version	NM_001366646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596093.18, AL162454.23 and AL137002.19. On Oct 13, 2018 this sequence version replaced XM_017020498.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.215256.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353575.1
CDS	80..3343
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366646.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366646.1

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001366646.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353575.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.

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CloneID	OHu84482
Clone ID Related Accession (Same CDS sequence)	NM_001366646.1 , XM_017020498.1 , NM_001366646.2
Accession Version	NM_001366646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL596093.18, AL162454.23 and AL137002.19. On Jun 1, 2019 this sequence version replaced NM_001366646.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.215256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353575.1
CDS	80..3343
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001366646.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366646.2

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537486.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535788.2 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84476
Clone ID Related Accession (Same CDS sequence)	XM_011537486.2
Accession Version	XM_011537486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGATGACA CGGTCACTAG CTCTGCCTCA GAAAGCTCTG CGCTTTCCAG AAAGCGCTTT 
ACCCTGCAGG GTTTTGCTAA CCTCAAAGGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA 
AAAGCCAAGC GGCATGAAGT AAAGAGCGAC CCGACTCCCT TTGGTGTGCG AGGTTGGAGC 
AAAACGTCCC ACTCACTGGA GGCACCTGAG GACGACGGGG GCTGGTCAAG TGCAGAGGAG 
CAGATTAACT CGTCCGACGC AGAGGAGGAC GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA 
GGTAAATACA CGGTCGTGGC GGACCACGAG AAGGGAGGCC CCGATGCGCT GCGCGTGAGG 
AGCGGGGACG TGGTGGAGCT GGTGCAGGAG GGCGACGAGG GCCTCTGGTA CGTCAGGGAC 
CCGACCACTG GCAAGGAGGG CTGGGTGCCG GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG 
TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC 
AACTCGTCCA GCTGCAGCGA GGGCGGCCAG GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535788.2
CDS	113..3829
Translation	MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCCGLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537486.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537486.2

ORF Insert Sequence:

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGATGACA CGGTCACTAG CTCTGCCTCA GAAAGCTCTG CGCTTTCCAG AAAGCGCTTT 
ACCCTGCAGG GTTTTGCTAA CCTCAAAGGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA 
AAAGCCAAGC GGCATGAAGT AAAGAGCGAC CCGACTCCCT TTGGTGTGCG AGGTTGGAGC 
AAAACGTCCC ACTCACTGGA GGCACCTGAG GACGACGGGG GCTGGTCAAG TGCAGAGGAG 
CAGATTAACT CGTCCGACGC AGAGGAGGAC GGCGGGTTGG GCCCCAAGAA GCTGGTTCCA 
GGTAAATACA CGGTCGTGGC GGACCACGAG AAGGGAGGCC CCGATGCGCT GCGCGTGAGG 
AGCGGGGACG TGGTGGAGCT GGTGCAGGAG GGCGACGAGG GCCTCTGGTA CGTCAGGGAC 
CCGACCACTG GCAAGGAGGG CTGGGTGCCG GCCAGCAGCC TGTCCGTCCG GCTCGGCCCG 
TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA GAGTCGAGCC CGGGGTCGGC CGTGCTGAGC 
AACTCGTCCA GCTGCAGCGA GGGCGGCCAG GCCCCCTTCT CCGACCTGCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020493.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875982.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

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CloneID	OHu84477
Clone ID Related Accession (Same CDS sequence)	XM_017020493.1
Accession Version	XM_017020493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGCTTCTC CTACCAGTCC TGACAAAAAA GCCAAGCGGC ATGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTGTGCGAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875982.1
CDS	113..3736
Translation	MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCCGLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020493.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020493.1

ORF Insert Sequence:

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGCTTCTC CTACCAGTCC TGACAAAAAA GCCAAGCGGC ATGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTGTGCGAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020494.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875983.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

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CloneID	OHu84478
Clone ID Related Accession (Same CDS sequence)	XM_017020494.1
Accession Version	XM_017020494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG GAAAGGCTGT CCGGATTTCC GTGGCGGAAA TGAAAAGCTA CTACCTGTAT 
TCTGAGTGGT GCTCCTGGCT GCTGTCCGTG GCTGAGGATG AAATCATGCA CCAGGACATC 
GTCCCGCTCT GTGCTGCCGA CATCCAGGAC CAGCTAAAGA AGCGCTTTGC TTACCTGTCC 
GGTGGGCGGG GGCAGGACGG AAGCCCGGTT ATCACCTTCC CTGACTACCC GGCCTTCAGC 
GAGATTCCGG ACAAGGAGTT CCAGAATGTC ATGACCTACC TCACCAGCAT CCCCAGCCTG 
CAGGACGCTG GCATCGGATT CATCCTGGTG ATAGACCGGC GACGGGACAA ATGGACCTCC 
GTGAAGGCGT CCGTCCTGCG CATCGCAGCA TCTTTCCCGG CAAACCTGCA GCTCGTCCTC 
GTGCTTCGCC CGACGGGTTT TTTCCAAAGG ACTCTCTCCG ACATCGCTTT CAAATTCAAT 
AGAGATGACT TTAAGATGAA GGTGCCGGTC ATAATGCTGA GCTCCGTACC AGACTTACAC 
GGTTACATCG ATAAGTCGCA GCTGACCGAG GACCTGGGTG GGACCCTGGA CTACTGCCAC 
TCCCGGTGGC TGTGCCAGCG CACGGCCATC GAAAGTTTCG CCCTCATGGT GAAGCAGACG 
GCTCAGATGC TGCAGTCCTT CGGGACCGAG CTGGCTGAAA CAGAGCTGCC CAATGACGTC 
CAGTCGACAA GCTCAGTGCT GTGTGCGCAC ACAGAGAAGA AGGACAAGGC GAAGGAGGAT 
TTGAGGCTGG CACTGAAAGA GGGGCACAGT GTCCTGGAGA GCCTCAGGGA GCTGCAGGCT 
GAGGGCTCAG AGCCCAGTGT GAACCAGGAC CAGCTTGACA ACCAGGCCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAACGA AACCGAGGCT GCCTTCGATG AGTTCTGGGC AAAGCATCAG 
CAGAAACTGG AGCAGTGTCT GCAGCTCCGG CACTTTGAGC AGGGCTTCCG GGAGGTCAAA 
GCCATCTTGG ACGCAGCGTC CCAGAAGATA GCAACCTTCA CAGACATCGG CAACAGCCTG 
GCGCATGTGG AGCACCTGCT GAGGGACCTG GCCAGCTTCG AGGAGAAATC AGGCGTGGCC 
GTGGAGAGGG CCCGGGCCCT GTCTCTGGAC GGCGAGCAGC TCATTGGGAA CAAGCACTAC 
GCGGTAGACT CCATCCGCCC AAAGTGCCAG GAGCTCCGGC ACCTCTGTGA CCAGTTCTCT 
GCGGAGATCG CAAGGAGGAG GGGGCTGCTC AGCAAGTCCC TGGAGCTGCA CCGCCGCCTG 
GAGACGTCCA TGAAGTGGTG TGATGAAGGG ATTTACCTGC TGGCCTCACA ACCTGTGGAC 
AAGTGCCAGT CCCAGGACGG CGCGGAGGCT GCCCTCCAGG AAATCGAGAA GTTTTTGGAG 
ACCGGTGCGG AAAATAAGAT CCAGGAGCTC AACGCGATTT ACAAGGAATA CGAATCCATC 
CTCAACCAAG ATCTCATGGA GCACGTGCGA AAGGTCTTCC AGAAGCAGGC AAGCATGGAG 
GAGGTGTTCC ACCGCAGGCA GGCCAGCCTG AAGAAGCTGG CGGCCAGGCA GACGCGGCCC 
GTGCAGCCGG TGGCCCCCAG ACCCGAGGCA CTGGCAAAGT CGCCCTGCCC CTCCCCAGGC 
ATTCGGCGAG GCTCTGAGAA CTCCAGCTCC GAGGGCGGTG CGCTCCGGAG AGGGCCCTAC 
CGGAGGGCCA AGAGTGAGAT GAGTGAGAGC CGGCAGGGCC GCGGCTCAGC GGGGGAGGAG 
GAGGAAAGCC TGGCCATCCT GCGCAGGCAC GTGATGAGCG AGCTCCTGGA CACAGAACGG 
GCCTACGTGG AGGAGCTGCT GTGCGTCCTG GAGGGCTACG CCGCGGAGAT GGATAACCCA 
CTGATGGCTC ACCTCCTGTC AACAGGCCTT CACAACAAGA AGGATGTTTT GTTTGGAAAC 
ATGGAGGAAA TCTATCACTT CCACAACAGG ATATTCCTCA GGGAGCTGGA AAACTACACT 
GACTGCCCAG AACTGGTTGG AAGATGCTTT CTGGAGAGGA TGGAAGATTT CCAGATCTAT 
GAGAAGTACT GTCAGAACAA GCCCCGCTCT GAGAGCCTGT GGAGACAGTG CTCCGACTGC 
CCGTTTTTCC AGGAATGCCA GAGAAAGCTG GACCACAAGC TGAGCCTGGA CTCCTACCTG 
CTGAAGCCAG TGCAGAGGAT CACCAAGTAC CAGCTGCTGC TCAAGGAAAT GCTGAAATAC 
AGCAGGAACT GCGAGGGGGC TGAGGACCTG CAGGAGGCGC TGAGCTCCAT CCTGGGCATC 
CTGAAGGCCG TGAACGACTC CATGCACCTC ATCGCTATCA CCGGCTATGA CGGGAATCTC 
GGCGACCTGG GCAAGCTGCT GATGCAGGGC TCGTTCAGCG TCTGGACCGA CCACAAGAGG 
GGCCACACCA AGGTGAAGGA GCTGGCCAGG TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG 
CACGAGAAGG CAGTGCTCTT CTGCAAGAAG AGGGAGGAGA ATGGGGAGGG GTATGAGAAA 
GCTCCCTCCT ACAGCTACAA GCAGTCCTTA AACATGGCTG CCGTTGGCAT TACGGAGAAC 
GTGAAGGGAG ATGCTAAGAA GTTCGAGATC TGGTACAACG CGCGCGAGGA GGTCTACATC 
GTCCAGGCGC CAACTCCTGA GATTAAAGCC GCGTGGGTGA ATGAAATTCG GAAAGTGCTG 
ACCAGCCAGC TGCAGGCTTG TAGAGAAGCC AGCCAGCACC GGGCGCTGGA GCAGTCACAG 
AGCCTGCCCC TGCCGGCCCC GACCAGCACC AGTCCCTCAA GAGGAAACTC AAGGAACATC 
AAGAAGCTGG AAGAAAGGAA AACAGACCCC CTAAGCCTGG AGGGATACGT CAGCTCAGCG 
CCACTGACAA AGCCCCCCGA AAAGGGCAAA GATGACACGG TCACTAGCTC TGCCTCAGAA 
AGCTCTGCGC TTTCCAGAAA GCGCTTTACC CTGCAGGGTT TTGCTAACCT CAAAGGTCAG 
AAAGCTTCTC CTACCAGTCC TGACAAAAAA GCCAAGCGGC ATGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTGTGCGAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875983.1
CDS	266..3829
Translation	MGDGKAVRISVAEMKSYYLYSEWCSWLLSVAEDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020494.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020494.1

ORF Insert Sequence:

ATGGGGGACG GAAAGGCTGT CCGGATTTCC GTGGCGGAAA TGAAAAGCTA CTACCTGTAT 
TCTGAGTGGT GCTCCTGGCT GCTGTCCGTG GCTGAGGATG AAATCATGCA CCAGGACATC 
GTCCCGCTCT GTGCTGCCGA CATCCAGGAC CAGCTAAAGA AGCGCTTTGC TTACCTGTCC 
GGTGGGCGGG GGCAGGACGG AAGCCCGGTT ATCACCTTCC CTGACTACCC GGCCTTCAGC 
GAGATTCCGG ACAAGGAGTT CCAGAATGTC ATGACCTACC TCACCAGCAT CCCCAGCCTG 
CAGGACGCTG GCATCGGATT CATCCTGGTG ATAGACCGGC GACGGGACAA ATGGACCTCC 
GTGAAGGCGT CCGTCCTGCG CATCGCAGCA TCTTTCCCGG CAAACCTGCA GCTCGTCCTC 
GTGCTTCGCC CGACGGGTTT TTTCCAAAGG ACTCTCTCCG ACATCGCTTT CAAATTCAAT 
AGAGATGACT TTAAGATGAA GGTGCCGGTC ATAATGCTGA GCTCCGTACC AGACTTACAC 
GGTTACATCG ATAAGTCGCA GCTGACCGAG GACCTGGGTG GGACCCTGGA CTACTGCCAC 
TCCCGGTGGC TGTGCCAGCG CACGGCCATC GAAAGTTTCG CCCTCATGGT GAAGCAGACG 
GCTCAGATGC TGCAGTCCTT CGGGACCGAG CTGGCTGAAA CAGAGCTGCC CAATGACGTC 
CAGTCGACAA GCTCAGTGCT GTGTGCGCAC ACAGAGAAGA AGGACAAGGC GAAGGAGGAT 
TTGAGGCTGG CACTGAAAGA GGGGCACAGT GTCCTGGAGA GCCTCAGGGA GCTGCAGGCT 
GAGGGCTCAG AGCCCAGTGT GAACCAGGAC CAGCTTGACA ACCAGGCCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAACGA AACCGAGGCT GCCTTCGATG AGTTCTGGGC AAAGCATCAG 
CAGAAACTGG AGCAGTGTCT GCAGCTCCGG CACTTTGAGC AGGGCTTCCG GGAGGTCAAA 
GCCATCTTGG ACGCAGCGTC CCAGAAGATA GCAACCTTCA CAGACATCGG CAACAGCCTG 
GCGCATGTGG AGCACCTGCT GAGGGACCTG GCCAGCTTCG AGGAGAAATC AGGCGTGGCC 
GTGGAGAGGG CCCGGGCCCT GTCTCTGGAC GGCGAGCAGC TCATTGGGAA CAAGCACTAC 
GCGGTAGACT CCATCCGCCC AAAGTGCCAG GAGCTCCGGC ACCTCTGTGA CCAGTTCTCT 
GCGGAGATCG CAAGGAGGAG GGGGCTGCTC AGCAAGTCCC TGGAGCTGCA CCGCCGCCTG 
GAGACGTCCA TGAAGTGGTG TGATGAAGGG ATTTACCTGC TGGCCTCACA ACCTGTGGAC 
AAGTGCCAGT CCCAGGACGG CGCGGAGGCT GCCCTCCAGG AAATCGAGAA GTTTTTGGAG 
ACCGGTGCGG AAAATAAGAT CCAGGAGCTC AACGCGATTT ACAAGGAATA CGAATCCATC 
CTCAACCAAG ATCTCATGGA GCACGTGCGA AAGGTCTTCC AGAAGCAGGC AAGCATGGAG 
GAGGTGTTCC ACCGCAGGCA GGCCAGCCTG AAGAAGCTGG CGGCCAGGCA GACGCGGCCC 
GTGCAGCCGG TGGCCCCCAG ACCCGAGGCA CTGGCAAAGT CGCCCTGCCC CTCCCCAGGC 
ATTCGGCGAG GCTCTGAGAA CTCCAGCTCC GAGGGCGGTG CGCTCCGGAG AGGGCCCTAC 
CGGAGGGCCA AGAGTGAGAT GAGTGAGAGC CGGCAGGGCC GCGGCTCAGC GGGGGAGGAG 
GAGGAAAGCC TGGCCATCCT GCGCAGGCAC GTGATGAGCG AGCTCCTGGA CACAGAACGG 
GCCTACGTGG AGGAGCTGCT GTGCGTCCTG GAGGGCTACG CCGCGGAGAT GGATAACCCA 
CTGATGGCTC ACCTCCTGTC AACAGGCCTT CACAACAAGA AGGATGTTTT GTTTGGAAAC 
ATGGAGGAAA TCTATCACTT CCACAACAGG ATATTCCTCA GGGAGCTGGA AAACTACACT 
GACTGCCCAG AACTGGTTGG AAGATGCTTT CTGGAGAGGA TGGAAGATTT CCAGATCTAT 
GAGAAGTACT GTCAGAACAA GCCCCGCTCT GAGAGCCTGT GGAGACAGTG CTCCGACTGC 
CCGTTTTTCC AGGAATGCCA GAGAAAGCTG GACCACAAGC TGAGCCTGGA CTCCTACCTG 
CTGAAGCCAG TGCAGAGGAT CACCAAGTAC CAGCTGCTGC TCAAGGAAAT GCTGAAATAC 
AGCAGGAACT GCGAGGGGGC TGAGGACCTG CAGGAGGCGC TGAGCTCCAT CCTGGGCATC 
CTGAAGGCCG TGAACGACTC CATGCACCTC ATCGCTATCA CCGGCTATGA CGGGAATCTC 
GGCGACCTGG GCAAGCTGCT GATGCAGGGC TCGTTCAGCG TCTGGACCGA CCACAAGAGG 
GGCCACACCA AGGTGAAGGA GCTGGCCAGG TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG 
CACGAGAAGG CAGTGCTCTT CTGCAAGAAG AGGGAGGAGA ATGGGGAGGG GTATGAGAAA 
GCTCCCTCCT ACAGCTACAA GCAGTCCTTA AACATGGCTG CCGTTGGCAT TACGGAGAAC 
GTGAAGGGAG ATGCTAAGAA GTTCGAGATC TGGTACAACG CGCGCGAGGA GGTCTACATC 
GTCCAGGCGC CAACTCCTGA GATTAAAGCC GCGTGGGTGA ATGAAATTCG GAAAGTGCTG 
ACCAGCCAGC TGCAGGCTTG TAGAGAAGCC AGCCAGCACC GGGCGCTGGA GCAGTCACAG 
AGCCTGCCCC TGCCGGCCCC GACCAGCACC AGTCCCTCAA GAGGAAACTC AAGGAACATC 
AAGAAGCTGG AAGAAAGGAA AACAGACCCC CTAAGCCTGG AGGGATACGT CAGCTCAGCG 
CCACTGACAA AGCCCCCCGA AAAGGGCAAA GATGACACGG TCACTAGCTC TGCCTCAGAA 
AGCTCTGCGC TTTCCAGAAA GCGCTTTACC CTGCAGGGTT TTGCTAACCT CAAAGGTCAG 
AAAGCTTCTC CTACCAGTCC TGACAAAAAA GCCAAGCGGC ATGAAGTAAA GAGCGACCCG 
ACTCCCTTTG GTGTGCGAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020496.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875985.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

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CloneID	OHu84480
Clone ID Related Accession (Same CDS sequence)	NM_001366645.1 , XM_017020496.1 , NM_001366645.2
Accession Version	XM_017020496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875985.1
CDS	91..3429
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020496.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020496.1

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020498.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875987.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.

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CloneID	OHu84482
Clone ID Related Accession (Same CDS sequence)	NM_001366646.1 , XM_017020498.1 , NM_001366646.2
Accession Version	XM_017020498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875987.1
CDS	91..3354
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_017020498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020498.1

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGG TTGGAGCAAA ACGTCCCACT CACTGGAGGC ACCTGAGGAC 
GACGGGGGCT GGTCAAGTGC AGAGGAGCAG ATTAACTCGT CCGACGCAGA GGAGGACGGC 
GGGTTGGGCC CCAAGAAGCT GGTTCCAGGT AAATACACGG TCGTGGCGGA CCACGAGAAG 
GGAGGCCCCG ATGCGCTGCG CGTGAGGAGC GGGGACGTGG TGGAGCTGGT GCAGGAGGGC 
GACGAGGGCC TCTGGTACGT CAGGGACCCG ACCACTGGCA AGGAGGGCTG GGTGCCGGCC 
AGCAGCCTGT CCGTCCGGCT CGGCCCGTCC GGCTCGGCCC AGTGCCTGAG CAGCTCAGAG 
TCGAGCCCGG GGTCGGCCGT GCTGAGCAAC TCGTCCAGCT GCAGCGAGGG CGGCCAGGCC 
CCCTTCTCCG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020499.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875988.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu84483
Clone ID Related Accession (Same CDS sequence)	XM_017020499.1 , XM_017020499.2
Accession Version	XM_017020499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGAGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875988.1
CDS	113..3244
Translation	MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCCGLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKEP

Target ORF information:

RefSeq Version	XM_017020499.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020499.1

ORF Insert Sequence:

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGAGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_017020499.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875988.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu84483
Clone ID Related Accession (Same CDS sequence)	XM_017020499.1 , XM_017020499.2
Accession Version	XM_017020499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGAGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875988.1
CDS	113..3244
Translation	MARVESPVPAARASLTGSCVLGQAMPLRGGAGPSPASHGPTHGPSDPRTCLPGRGAGGMRPHGRGALGCCGLCSFYTCHGAAGDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKEP

Target ORF information:

RefSeq Version	XM_017020499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020499.2

ORF Insert Sequence:

ATGGCGAGGG TGGAGAGCCC CGTGCCTGCA GCGCGCGCTT CCCTCACTGG GTCCTGCGTC 
CTTGGGCAGG CGATGCCCCT GCGGGGAGGG GCTGGTCCAT CCCCGGCCAG CCACGGACCC 
ACGCATGGAC CCAGCGACCC ACGGACCTGC TTACCTGGGC GCGGCGCGGG TGGCATGCGG 
CCACACGGAA GGGGCGCGCT GGGCTGCTGC GGCCTCTGCA GCTTCTACAC CTGCCACGGG 
GCGGCCGGAG ATGAAATCAT GCACCAGGAC ATCGTCCCGC TCTGTGCTGC CGACATCCAG 
GACCAGCTAA AGAAGCGCTT TGCTTACCTG TCCGGTGGGC GGGGGCAGGA CGGAAGCCCG 
GTTATCACCT TCCCTGACTA CCCGGCCTTC AGCGAGATTC CGGACAAGGA GTTCCAGAAT 
GTCATGACCT ACCTCACCAG CATCCCCAGC CTGCAGGACG CTGGCATCGG ATTCATCCTG 
GTGATAGACC GGCGACGGGA CAAATGGACC TCCGTGAAGG CGTCCGTCCT GCGCATCGCA 
GCATCTTTCC CGGCAAACCT GCAGCTCGTC CTCGTGCTTC GCCCGACGGG TTTTTTCCAA 
AGGACTCTCT CCGACATCGC TTTCAAATTC AATAGAGATG ACTTTAAGAT GAAGGTGCCG 
GTCATAATGC TGAGCTCCGT ACCAGACTTA CACGGTTACA TCGATAAGTC GCAGCTGACC 
GAGGACCTGG GTGGGACCCT GGACTACTGC CACTCCCGGT GGCTGTGCCA GCGCACGGCC 
ATCGAAAGTT TCGCCCTCAT GGTGAAGCAG ACGGCTCAGA TGCTGCAGTC CTTCGGGACC 
GAGCTGGCTG AAACAGAGCT GCCCAATGAC GTCCAGTCGA CAAGCTCAGT GCTGTGTGCG 
CACACAGAGA AGAAGGACAA GGCGAAGGAG GATTTGAGGC TGGCACTGAA AGAGGGGCAC 
AGTGTCCTGG AGAGCCTCAG GGAGCTGCAG GCTGAGGGCT CAGAGCCCAG TGTGAACCAG 
GACCAGCTTG ACAACCAGGC CACCGTGCAG AGGCTCCTGG CCCAGCTGAA CGAAACCGAG 
GCTGCCTTCG ATGAGTTCTG GGCAAAGCAT CAGCAGAAAC TGGAGCAGTG TCTGCAGCTC 
CGGCACTTTG AGCAGGGCTT CCGGGAGGTC AAAGCCATCT TGGACGCAGC GTCCCAGAAG 
ATAGCAACCT TCACAGACAT CGGCAACAGC CTGGCGCATG TGGAGCACCT GCTGAGGGAC 
CTGGCCAGCT TCGAGGAGAA ATCAGGCGTG GCCGTGGAGA GGGCCCGGGC CCTGTCTCTG 
GACGGCGAGC AGCTCATTGG GAACAAGCAC TACGCGGTAG ACTCCATCCG CCCAAAGTGC 
CAGGAGCTCC GGCACCTCTG TGACCAGTTC TCTGCGGAGA TCGCAAGGAG GAGGGGGCTG 
CTCAGCAAGT CCCTGGAGCT GCACCGCCGC CTGGAGACGT CCATGAAGTG GTGTGATGAA 
GGGATTTACC TGCTGGCCTC ACAACCTGTG GACAAGTGCC AGTCCCAGGA CGGCGCGGAG 
GCTGCCCTCC AGGAAATCGA GAAGTTTTTG GAGACCGGTG CGGAAAATAA GATCCAGGAG 
CTCAACGCGA TTTACAAGGA ATACGAATCC ATCCTCAACC AAGATCTCAT GGAGCACGTG 
CGAAAGGTCT TCCAGAAGCA GGCAAGCATG GAGGAGGTGT TCCACCGCAG GCAGGCCAGC 
CTGAAGAAGC TGGCGGCCAG GCAGACGCGG CCCGTGCAGC CGGTGGCCCC CAGACCCGAG 
GCACTGGCAA AGTCGCCCTG CCCCTCCCCA GGCATTCGGC GAGGCTCTGA GAACTCCAGC 
TCCGAGGGCG GTGCGCTCCG GAGAGGGCCC TACCGGAGGG CCAAGAGTGA GATGAGTGAG 
AGCCGGCAGG GCCGCGGCTC AGCGGGGGAG GAGGAGGAAA GCCTGGCCAT CCTGCGCAGG 
CACGTGATGA GCGAGCTCCT GGACACAGAA CGGGCCTACG TGGAGGAGCT GCTGTGCGTC 
CTGGAGGGCT ACGCCGCGGA GATGGATAAC CCACTGATGG CTCACCTCCT GTCAACAGGC 
CTTCACAACA AGAAGGATGT TTTGTTTGGA AACATGGAGG AAATCTATCA CTTCCACAAC 
AGGATATTCC TCAGGGAGCT GGAAAACTAC ACTGACTGCC CAGAACTGGT TGGAAGATGC 
TTTCTGGAGA GGATGGAAGA TTTCCAGATC TATGAGAAGT ACTGTCAGAA CAAGCCCCGC 
TCTGAGAGCC TGTGGAGACA GTGCTCCGAC TGCCCGTTTT TCCAGGAATG CCAGAGAAAG 
CTGGACCACA AGCTGAGCCT GGACTCCTAC CTGCTGAAGC CAGTGCAGAG GATCACCAAG 
TACCAGCTGC TGCTCAAGGA AATGCTGAAA TACAGCAGGA ACTGCGAGGG GGCTGAGGAC 
CTGCAGGAGG CGCTGAGCTC CATCCTGGGC ATCCTGAAGG CCGTGAACGA CTCCATGCAC 
CTCATCGCTA TCACCGGCTA TGACGGGAAT CTCGGCGACC TGGGCAAGCT GCTGATGCAG 
GGCTCGTTCA GCGTCTGGAC CGACCACAAG AGGGGCCACA CCAAGGTGAA GGAGCTGGCC 
AGGTTCAAGC CCATGCAGCG GCACCTGTTC CTGCACGAGA AGGCAGTGCT CTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAAGCTCCCT CCTACAGCTA CAAGCAGTCC 
TTAAACATGG CTGCCGTTGG CATTACGGAG AACGTGAAGG GAGATGCTAA GAAGTTCGAG 
ATCTGGTACA ACGCGCGCGA GGAGGTCTAC ATCGTCCAGG CGCCAACTCC TGAGATTAAA 
GCCGCGTGGG TGAATGAAAT TCGGAAAGTG CTGACCAGCC AGCTGCAGGC TTGTAGAGAA 
GCCAGCCAGC ACCGGGCGCT GGAGCAGTCA CAGAGCCTGC CCCTGCCGGC CCCGACCAGC 
ACCAGTCCCT CAAGAGGAAA CTCAAGGAAC ATCAAGAAGC TGGAAGAAAG GAAAACAGAC 
CCCCTAAGCC TGGAGGGATA CGTCAGCTCA GCGCCACTGA CAAAGCCCCC CGAAAAGGGC 
AAAGAGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_024979.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079255.4 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 2, mRNA.

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CloneID	OHu22048
Clone ID Related Accession (Same CDS sequence)	NM_024979.4
Accession Version	NM_024979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476653.1, AK295047.1, AL162454.23, AL137002.19 and AW241629.1. On Jul 2, 2011 this sequence version replaced NM_024979.3. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in an isoform (b) with a distinct and shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079255.4
CDS	198..3569
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_024979.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024979.4

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001112732.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106203.2 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.

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CloneID	OHu22268
Clone ID Related Accession (Same CDS sequence)	NM_001112732.3 , NM_001112732.2
Accession Version	NM_001112732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356740.19, AK295670.1, AL137002.19 and AW241629.1. On Nov 22, 2018 this sequence version replaced NM_001112732.2. Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.220663.1, SRR1660805.67443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000535094.7/ ENSP00000440374.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106203.2
CDS	167..3544
Translation	MRFWLRTEEMALEEMVQRLNAVSKHTDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001112732.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112732.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( NM_001112732.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106203.2 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.

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CloneID	OHu22268
Clone ID Related Accession (Same CDS sequence)	NM_001112732.3 , NM_001112732.2
Accession Version	NM_001112732.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356740.19, AK295670.1, AL137002.19 and AW241629.1. On Jul 2, 2011 this sequence version replaced NM_001112732.1. Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.220663.1, SRR1660805.67443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106203.2
CDS	160..3537
Translation	MRFWLRTEEMALEEMVQRLNAVSKHTDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	NM_001112732.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112732.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535784.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu65832
Clone ID Related Accession (Same CDS sequence)	XM_011537482.1 , XM_011537483.2
Accession Version	XM_011537482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACT GTTGGAGATT TATCCTGTGC AAGAGACCTG GAAGCAACAG TTATTCTTCC 
CCACAACGGC CCAATGAGGC CAAAAAGGAG GAAACGGATC ACCAAATTGA TGTGTCTGAC 
GTCATCAGGC TTGTTCAAGA CACACCGGAG GCGACGGCCA TGGCCACAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCTGCC GACATCCAGG ACCAGCTAAA GAAGCGCTTT 
GCTTACCTGT CCGGTGGGCG GGGGCAGGAC GGAAGCCCGG TTATCACCTT CCCTGACTAC 
CCGGCCTTCA GCGAGATTCC GGACAAGGAG TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGA TTCATCCTGG TGATAGACCG GCGACGGGAC 
AAATGGACCT CCGTGAAGGC GTCCGTCCTG CGCATCGCAG CATCTTTCCC GGCAAACCTG 
CAGCTCGTCC TCGTGCTTCG CCCGACGGGT TTTTTCCAAA GGACTCTCTC CGACATCGCT 
TTCAAATTCA ATAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCCGTA 
CCAGACTTAC ACGGTTACAT CGATAAGTCG CAGCTGACCG AGGACCTGGG TGGGACCCTG 
GACTACTGCC ACTCCCGGTG GCTGTGCCAG CGCACGGCCA TCGAAAGTTT CGCCCTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACCG AGCTGGCTGA AACAGAGCTG 
CCCAATGACG TCCAGTCGAC AAGCTCAGTG CTGTGTGCGC ACACAGAGAA GAAGGACAAG 
GCGAAGGAGG ATTTGAGGCT GGCACTGAAA GAGGGGCACA GTGTCCTGGA GAGCCTCAGG 
GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT GTGAACCAGG ACCAGCTTGA CAACCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC GAAACCGAGG CTGCCTTCGA TGAGTTCTGG 
GCAAAGCATC AGCAGAAACT GGAGCAGTGT CTGCAGCTCC GGCACTTTGA GCAGGGCTTC 
CGGGAGGTCA AAGCCATCTT GGACGCAGCG TCCCAGAAGA TAGCAACCTT CACAGACATC 
GGCAACAGCC TGGCGCATGT GGAGCACCTG CTGAGGGACC TGGCCAGCTT CGAGGAGAAA 
TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC CTGTCTCTGG ACGGCGAGCA GCTCATTGGG 
AACAAGCACT ACGCGGTAGA CTCCATCCGC CCAAAGTGCC AGGAGCTCCG GCACCTCTGT 
GACCAGTTCT CTGCGGAGAT CGCAAGGAGG AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG 
CACCGCCGCC TGGAGACGTC CATGAAGTGG TGTGATGAAG GGATTTACCT GCTGGCCTCA 
CAACCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCGGAGG CTGCCCTCCA GGAAATCGAG 
AAGTTTTTGG AGACCGGTGC GGAAAATAAG ATCCAGGAGC TCAACGCGAT TTACAAGGAA 
TACGAATCCA TCCTCAACCA AGATCTCATG GAGCACGTGC GAAAGGTCTT CCAGAAGCAG 
GCAAGCATGG AGGAGGTGTT CCACCGCAGG CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC AGACCCGAGG CACTGGCAAA GTCGCCCTGC 
CCCTCCCCAG GCATTCGGCG AGGCTCTGAG AACTCCAGCT CCGAGGGCGG TGCGCTCCGG 
AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA 
GCGGGGGAGG AGGAGGAAAG CCTGGCCATC CTGCGCAGGC ACGTGATGAG CGAGCTCCTG 
GACACAGAAC GGGCCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGATAACC CACTGATGGC TCACCTCCTG TCAACAGGCC TTCACAACAA GAAGGATGTT 
TTGTTTGGAA ACATGGAGGA AATCTATCAC TTCCACAACA GGATATTCCT CAGGGAGCTG 
GAAAACTACA CTGACTGCCC AGAACTGGTT GGAAGATGCT TTCTGGAGAG GATGGAAGAT 
TTCCAGATCT ATGAGAAGTA CTGTCAGAAC AAGCCCCGCT CTGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCGTTTTT CCAGGAATGC CAGAGAAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTGCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG GCTGAGGACC TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTGAAGGC CGTGAACGAC TCCATGCACC TCATCGCTAT CACCGGCTAT 
GACGGGAATC TCGGCGACCT GGGCAAGCTG CTGATGCAGG GCTCGTTCAG CGTCTGGACC 
GACCACAAGA GGGGCCACAC CAAGGTGAAG GAGCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCAGTGCTC TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AAGCTCCCTC CTACAGCTAC AAGCAGTCCT TAAACATGGC TGCCGTTGGC 
ATTACGGAGA ACGTGAAGGG AGATGCTAAG AAGTTCGAGA TCTGGTACAA CGCGCGCGAG 
GAGGTCTACA TCGTCCAGGC GCCAACTCCT GAGATTAAAG CCGCGTGGGT GAATGAAATT 
CGGAAAGTGC TGACCAGCCA GCTGCAGGCT TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG 
GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC CCGACCAGCA CCAGTCCCTC AAGAGGAAAC 
TCAAGGAACA TCAAGAAGCT GGAAGAAAGG AAAACAGACC CCCTAAGCCT GGAGGGATAC 
GTCAGCTCAG CGCCACTGAC AAAGCCCCCC GAAAAGGGCA AAGATGACAC GGTCACTAGC 
TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCCAAGCG GCATGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTGTGCGA GGTTGGAGCA AAACGTCCCA CTCACTGGAG 
GCACCTGAGG ACGACGGGGG CTGGTCAAGT GCAGAGGAGC AGATTAACTC GTCCGACGCA 
GAGGAGGACG GCGGGTTGGG CCCCAAGAAG CTGGTTCCAG GTAAATACAC GGTCGTGGCG 
GACCACGAGA AGGGAGGCCC CGATGCGCTG CGCGTGAGGA GCGGGGACGT GGTGGAGCTG 
GTGCAGGAGG GCGACGAGGG CCTCTGGTAC GTCAGGGACC CGACCACTGG CAAGGAGGGC 
TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG 
AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG 
GGCGGCCAGG CCCCCTTCTC CGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535784.1
CDS	59..3694
Translation	MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537482.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537482.1

ORF Insert Sequence:

ATGTTTGACT GTTGGAGATT TATCCTGTGC AAGAGACCTG GAAGCAACAG TTATTCTTCC 
CCACAACGGC CCAATGAGGC CAAAAAGGAG GAAACGGATC ACCAAATTGA TGTGTCTGAC 
GTCATCAGGC TTGTTCAAGA CACACCGGAG GCGACGGCCA TGGCCACAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCTGCC GACATCCAGG ACCAGCTAAA GAAGCGCTTT 
GCTTACCTGT CCGGTGGGCG GGGGCAGGAC GGAAGCCCGG TTATCACCTT CCCTGACTAC 
CCGGCCTTCA GCGAGATTCC GGACAAGGAG TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGA TTCATCCTGG TGATAGACCG GCGACGGGAC 
AAATGGACCT CCGTGAAGGC GTCCGTCCTG CGCATCGCAG CATCTTTCCC GGCAAACCTG 
CAGCTCGTCC TCGTGCTTCG CCCGACGGGT TTTTTCCAAA GGACTCTCTC CGACATCGCT 
TTCAAATTCA ATAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCCGTA 
CCAGACTTAC ACGGTTACAT CGATAAGTCG CAGCTGACCG AGGACCTGGG TGGGACCCTG 
GACTACTGCC ACTCCCGGTG GCTGTGCCAG CGCACGGCCA TCGAAAGTTT CGCCCTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACCG AGCTGGCTGA AACAGAGCTG 
CCCAATGACG TCCAGTCGAC AAGCTCAGTG CTGTGTGCGC ACACAGAGAA GAAGGACAAG 
GCGAAGGAGG ATTTGAGGCT GGCACTGAAA GAGGGGCACA GTGTCCTGGA GAGCCTCAGG 
GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT GTGAACCAGG ACCAGCTTGA CAACCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC GAAACCGAGG CTGCCTTCGA TGAGTTCTGG 
GCAAAGCATC AGCAGAAACT GGAGCAGTGT CTGCAGCTCC GGCACTTTGA GCAGGGCTTC 
CGGGAGGTCA AAGCCATCTT GGACGCAGCG TCCCAGAAGA TAGCAACCTT CACAGACATC 
GGCAACAGCC TGGCGCATGT GGAGCACCTG CTGAGGGACC TGGCCAGCTT CGAGGAGAAA 
TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC CTGTCTCTGG ACGGCGAGCA GCTCATTGGG 
AACAAGCACT ACGCGGTAGA CTCCATCCGC CCAAAGTGCC AGGAGCTCCG GCACCTCTGT 
GACCAGTTCT CTGCGGAGAT CGCAAGGAGG AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG 
CACCGCCGCC TGGAGACGTC CATGAAGTGG TGTGATGAAG GGATTTACCT GCTGGCCTCA 
CAACCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCGGAGG CTGCCCTCCA GGAAATCGAG 
AAGTTTTTGG AGACCGGTGC GGAAAATAAG ATCCAGGAGC TCAACGCGAT TTACAAGGAA 
TACGAATCCA TCCTCAACCA AGATCTCATG GAGCACGTGC GAAAGGTCTT CCAGAAGCAG 
GCAAGCATGG AGGAGGTGTT CCACCGCAGG CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC AGACCCGAGG CACTGGCAAA GTCGCCCTGC 
CCCTCCCCAG GCATTCGGCG AGGCTCTGAG AACTCCAGCT CCGAGGGCGG TGCGCTCCGG 
AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA 
GCGGGGGAGG AGGAGGAAAG CCTGGCCATC CTGCGCAGGC ACGTGATGAG CGAGCTCCTG 
GACACAGAAC GGGCCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGATAACC CACTGATGGC TCACCTCCTG TCAACAGGCC TTCACAACAA GAAGGATGTT 
TTGTTTGGAA ACATGGAGGA AATCTATCAC TTCCACAACA GGATATTCCT CAGGGAGCTG 
GAAAACTACA CTGACTGCCC AGAACTGGTT GGAAGATGCT TTCTGGAGAG GATGGAAGAT 
TTCCAGATCT ATGAGAAGTA CTGTCAGAAC AAGCCCCGCT CTGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCGTTTTT CCAGGAATGC CAGAGAAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTGCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG GCTGAGGACC TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTGAAGGC CGTGAACGAC TCCATGCACC TCATCGCTAT CACCGGCTAT 
GACGGGAATC TCGGCGACCT GGGCAAGCTG CTGATGCAGG GCTCGTTCAG CGTCTGGACC 
GACCACAAGA GGGGCCACAC CAAGGTGAAG GAGCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCAGTGCTC TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AAGCTCCCTC CTACAGCTAC AAGCAGTCCT TAAACATGGC TGCCGTTGGC 
ATTACGGAGA ACGTGAAGGG AGATGCTAAG AAGTTCGAGA TCTGGTACAA CGCGCGCGAG 
GAGGTCTACA TCGTCCAGGC GCCAACTCCT GAGATTAAAG CCGCGTGGGT GAATGAAATT 
CGGAAAGTGC TGACCAGCCA GCTGCAGGCT TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG 
GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC CCGACCAGCA CCAGTCCCTC AAGAGGAAAC 
TCAAGGAACA TCAAGAAGCT GGAAGAAAGG AAAACAGACC CCCTAAGCCT GGAGGGATAC 
GTCAGCTCAG CGCCACTGAC AAAGCCCCCC GAAAAGGGCA AAGATGACAC GGTCACTAGC 
TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCCAAGCG GCATGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTGTGCGA GGTTGGAGCA AAACGTCCCA CTCACTGGAG 
GCACCTGAGG ACGACGGGGG CTGGTCAAGT GCAGAGGAGC AGATTAACTC GTCCGACGCA 
GAGGAGGACG GCGGGTTGGG CCCCAAGAAG CTGGTTCCAG GTAAATACAC GGTCGTGGCG 
GACCACGAGA AGGGAGGCCC CGATGCGCTG CGCGTGAGGA GCGGGGACGT GGTGGAGCTG 
GTGCAGGAGG GCGACGAGGG CCTCTGGTAC GTCAGGGACC CGACCACTGG CAAGGAGGGC 
TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG 
AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG 
GGCGGCCAGG CCCCCTTCTC CGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537483.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535785.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu65832
Clone ID Related Accession (Same CDS sequence)	XM_011537482.1 , XM_011537483.2
Accession Version	XM_011537483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACT GTTGGAGATT TATCCTGTGC AAGAGACCTG GAAGCAACAG TTATTCTTCC 
CCACAACGGC CCAATGAGGC CAAAAAGGAG GAAACGGATC ACCAAATTGA TGTGTCTGAC 
GTCATCAGGC TTGTTCAAGA CACACCGGAG GCGACGGCCA TGGCCACAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCTGCC GACATCCAGG ACCAGCTAAA GAAGCGCTTT 
GCTTACCTGT CCGGTGGGCG GGGGCAGGAC GGAAGCCCGG TTATCACCTT CCCTGACTAC 
CCGGCCTTCA GCGAGATTCC GGACAAGGAG TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGA TTCATCCTGG TGATAGACCG GCGACGGGAC 
AAATGGACCT CCGTGAAGGC GTCCGTCCTG CGCATCGCAG CATCTTTCCC GGCAAACCTG 
CAGCTCGTCC TCGTGCTTCG CCCGACGGGT TTTTTCCAAA GGACTCTCTC CGACATCGCT 
TTCAAATTCA ATAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCCGTA 
CCAGACTTAC ACGGTTACAT CGATAAGTCG CAGCTGACCG AGGACCTGGG TGGGACCCTG 
GACTACTGCC ACTCCCGGTG GCTGTGCCAG CGCACGGCCA TCGAAAGTTT CGCCCTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACCG AGCTGGCTGA AACAGAGCTG 
CCCAATGACG TCCAGTCGAC AAGCTCAGTG CTGTGTGCGC ACACAGAGAA GAAGGACAAG 
GCGAAGGAGG ATTTGAGGCT GGCACTGAAA GAGGGGCACA GTGTCCTGGA GAGCCTCAGG 
GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT GTGAACCAGG ACCAGCTTGA CAACCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC GAAACCGAGG CTGCCTTCGA TGAGTTCTGG 
GCAAAGCATC AGCAGAAACT GGAGCAGTGT CTGCAGCTCC GGCACTTTGA GCAGGGCTTC 
CGGGAGGTCA AAGCCATCTT GGACGCAGCG TCCCAGAAGA TAGCAACCTT CACAGACATC 
GGCAACAGCC TGGCGCATGT GGAGCACCTG CTGAGGGACC TGGCCAGCTT CGAGGAGAAA 
TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC CTGTCTCTGG ACGGCGAGCA GCTCATTGGG 
AACAAGCACT ACGCGGTAGA CTCCATCCGC CCAAAGTGCC AGGAGCTCCG GCACCTCTGT 
GACCAGTTCT CTGCGGAGAT CGCAAGGAGG AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG 
CACCGCCGCC TGGAGACGTC CATGAAGTGG TGTGATGAAG GGATTTACCT GCTGGCCTCA 
CAACCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCGGAGG CTGCCCTCCA GGAAATCGAG 
AAGTTTTTGG AGACCGGTGC GGAAAATAAG ATCCAGGAGC TCAACGCGAT TTACAAGGAA 
TACGAATCCA TCCTCAACCA AGATCTCATG GAGCACGTGC GAAAGGTCTT CCAGAAGCAG 
GCAAGCATGG AGGAGGTGTT CCACCGCAGG CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC AGACCCGAGG CACTGGCAAA GTCGCCCTGC 
CCCTCCCCAG GCATTCGGCG AGGCTCTGAG AACTCCAGCT CCGAGGGCGG TGCGCTCCGG 
AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA 
GCGGGGGAGG AGGAGGAAAG CCTGGCCATC CTGCGCAGGC ACGTGATGAG CGAGCTCCTG 
GACACAGAAC GGGCCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGATAACC CACTGATGGC TCACCTCCTG TCAACAGGCC TTCACAACAA GAAGGATGTT 
TTGTTTGGAA ACATGGAGGA AATCTATCAC TTCCACAACA GGATATTCCT CAGGGAGCTG 
GAAAACTACA CTGACTGCCC AGAACTGGTT GGAAGATGCT TTCTGGAGAG GATGGAAGAT 
TTCCAGATCT ATGAGAAGTA CTGTCAGAAC AAGCCCCGCT CTGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCGTTTTT CCAGGAATGC CAGAGAAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTGCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG GCTGAGGACC TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTGAAGGC CGTGAACGAC TCCATGCACC TCATCGCTAT CACCGGCTAT 
GACGGGAATC TCGGCGACCT GGGCAAGCTG CTGATGCAGG GCTCGTTCAG CGTCTGGACC 
GACCACAAGA GGGGCCACAC CAAGGTGAAG GAGCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCAGTGCTC TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AAGCTCCCTC CTACAGCTAC AAGCAGTCCT TAAACATGGC TGCCGTTGGC 
ATTACGGAGA ACGTGAAGGG AGATGCTAAG AAGTTCGAGA TCTGGTACAA CGCGCGCGAG 
GAGGTCTACA TCGTCCAGGC GCCAACTCCT GAGATTAAAG CCGCGTGGGT GAATGAAATT 
CGGAAAGTGC TGACCAGCCA GCTGCAGGCT TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG 
GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC CCGACCAGCA CCAGTCCCTC AAGAGGAAAC 
TCAAGGAACA TCAAGAAGCT GGAAGAAAGG AAAACAGACC CCCTAAGCCT GGAGGGATAC 
GTCAGCTCAG CGCCACTGAC AAAGCCCCCC GAAAAGGGCA AAGATGACAC GGTCACTAGC 
TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCCAAGCG GCATGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTGTGCGA GGTTGGAGCA AAACGTCCCA CTCACTGGAG 
GCACCTGAGG ACGACGGGGG CTGGTCAAGT GCAGAGGAGC AGATTAACTC GTCCGACGCA 
GAGGAGGACG GCGGGTTGGG CCCCAAGAAG CTGGTTCCAG GTAAATACAC GGTCGTGGCG 
GACCACGAGA AGGGAGGCCC CGATGCGCTG CGCGTGAGGA GCGGGGACGT GGTGGAGCTG 
GTGCAGGAGG GCGACGAGGG CCTCTGGTAC GTCAGGGACC CGACCACTGG CAAGGAGGGC 
TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG 
AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG 
GGCGGCCAGG CCCCCTTCTC CGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535785.1
CDS	179..3814
Translation	MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537483.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537483.2

ORF Insert Sequence:

ATGTTTGACT GTTGGAGATT TATCCTGTGC AAGAGACCTG GAAGCAACAG TTATTCTTCC 
CCACAACGGC CCAATGAGGC CAAAAAGGAG GAAACGGATC ACCAAATTGA TGTGTCTGAC 
GTCATCAGGC TTGTTCAAGA CACACCGGAG GCGACGGCCA TGGCCACAGA TGAAATCATG 
CACCAGGACA TCGTCCCGCT CTGTGCTGCC GACATCCAGG ACCAGCTAAA GAAGCGCTTT 
GCTTACCTGT CCGGTGGGCG GGGGCAGGAC GGAAGCCCGG TTATCACCTT CCCTGACTAC 
CCGGCCTTCA GCGAGATTCC GGACAAGGAG TTCCAGAATG TCATGACCTA CCTCACCAGC 
ATCCCCAGCC TGCAGGACGC TGGCATCGGA TTCATCCTGG TGATAGACCG GCGACGGGAC 
AAATGGACCT CCGTGAAGGC GTCCGTCCTG CGCATCGCAG CATCTTTCCC GGCAAACCTG 
CAGCTCGTCC TCGTGCTTCG CCCGACGGGT TTTTTCCAAA GGACTCTCTC CGACATCGCT 
TTCAAATTCA ATAGAGATGA CTTTAAGATG AAGGTGCCGG TCATAATGCT GAGCTCCGTA 
CCAGACTTAC ACGGTTACAT CGATAAGTCG CAGCTGACCG AGGACCTGGG TGGGACCCTG 
GACTACTGCC ACTCCCGGTG GCTGTGCCAG CGCACGGCCA TCGAAAGTTT CGCCCTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACCG AGCTGGCTGA AACAGAGCTG 
CCCAATGACG TCCAGTCGAC AAGCTCAGTG CTGTGTGCGC ACACAGAGAA GAAGGACAAG 
GCGAAGGAGG ATTTGAGGCT GGCACTGAAA GAGGGGCACA GTGTCCTGGA GAGCCTCAGG 
GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT GTGAACCAGG ACCAGCTTGA CAACCAGGCC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC GAAACCGAGG CTGCCTTCGA TGAGTTCTGG 
GCAAAGCATC AGCAGAAACT GGAGCAGTGT CTGCAGCTCC GGCACTTTGA GCAGGGCTTC 
CGGGAGGTCA AAGCCATCTT GGACGCAGCG TCCCAGAAGA TAGCAACCTT CACAGACATC 
GGCAACAGCC TGGCGCATGT GGAGCACCTG CTGAGGGACC TGGCCAGCTT CGAGGAGAAA 
TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC CTGTCTCTGG ACGGCGAGCA GCTCATTGGG 
AACAAGCACT ACGCGGTAGA CTCCATCCGC CCAAAGTGCC AGGAGCTCCG GCACCTCTGT 
GACCAGTTCT CTGCGGAGAT CGCAAGGAGG AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG 
CACCGCCGCC TGGAGACGTC CATGAAGTGG TGTGATGAAG GGATTTACCT GCTGGCCTCA 
CAACCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCGGAGG CTGCCCTCCA GGAAATCGAG 
AAGTTTTTGG AGACCGGTGC GGAAAATAAG ATCCAGGAGC TCAACGCGAT TTACAAGGAA 
TACGAATCCA TCCTCAACCA AGATCTCATG GAGCACGTGC GAAAGGTCTT CCAGAAGCAG 
GCAAGCATGG AGGAGGTGTT CCACCGCAGG CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC AGACCCGAGG CACTGGCAAA GTCGCCCTGC 
CCCTCCCCAG GCATTCGGCG AGGCTCTGAG AACTCCAGCT CCGAGGGCGG TGCGCTCCGG 
AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA 
GCGGGGGAGG AGGAGGAAAG CCTGGCCATC CTGCGCAGGC ACGTGATGAG CGAGCTCCTG 
GACACAGAAC GGGCCTACGT GGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG 
ATGGATAACC CACTGATGGC TCACCTCCTG TCAACAGGCC TTCACAACAA GAAGGATGTT 
TTGTTTGGAA ACATGGAGGA AATCTATCAC TTCCACAACA GGATATTCCT CAGGGAGCTG 
GAAAACTACA CTGACTGCCC AGAACTGGTT GGAAGATGCT TTCTGGAGAG GATGGAAGAT 
TTCCAGATCT ATGAGAAGTA CTGTCAGAAC AAGCCCCGCT CTGAGAGCCT GTGGAGACAG 
TGCTCCGACT GCCCGTTTTT CCAGGAATGC CAGAGAAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTGCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG GCTGAGGACC TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTGAAGGC CGTGAACGAC TCCATGCACC TCATCGCTAT CACCGGCTAT 
GACGGGAATC TCGGCGACCT GGGCAAGCTG CTGATGCAGG GCTCGTTCAG CGTCTGGACC 
GACCACAAGA GGGGCCACAC CAAGGTGAAG GAGCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCAGTGCTC TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AAGCTCCCTC CTACAGCTAC AAGCAGTCCT TAAACATGGC TGCCGTTGGC 
ATTACGGAGA ACGTGAAGGG AGATGCTAAG AAGTTCGAGA TCTGGTACAA CGCGCGCGAG 
GAGGTCTACA TCGTCCAGGC GCCAACTCCT GAGATTAAAG CCGCGTGGGT GAATGAAATT 
CGGAAAGTGC TGACCAGCCA GCTGCAGGCT TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG 
GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC CCGACCAGCA CCAGTCCCTC AAGAGGAAAC 
TCAAGGAACA TCAAGAAGCT GGAAGAAAGG AAAACAGACC CCCTAAGCCT GGAGGGATAC 
GTCAGCTCAG CGCCACTGAC AAAGCCCCCC GAAAAGGGCA AAGATGACAC GGTCACTAGC 
TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCCAAGCG GCATGAAGTA 
AAGAGCGACC CGACTCCCTT TGGTGTGCGA GGTTGGAGCA AAACGTCCCA CTCACTGGAG 
GCACCTGAGG ACGACGGGGG CTGGTCAAGT GCAGAGGAGC AGATTAACTC GTCCGACGCA 
GAGGAGGACG GCGGGTTGGG CCCCAAGAAG CTGGTTCCAG GTAAATACAC GGTCGTGGCG 
GACCACGAGA AGGGAGGCCC CGATGCGCTG CGCGTGAGGA GCGGGGACGT GGTGGAGCTG 
GTGCAGGAGG GCGACGAGGG CCTCTGGTAC GTCAGGGACC CGACCACTGG CAAGGAGGGC 
TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG 
AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG 
GGCGGCCAGG CCCCCTTCTC CGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535786.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

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CloneID	OHu65833
Clone ID Related Accession (Same CDS sequence)	XM_011537484.1
Accession Version	XM_011537484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGATGAC ACGGTCACTA GCTCTGCCTC AGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCTACCA GTCCTGACAA AAAAGCCAAG CGGCATGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTGTGC GAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535786.1
CDS	584..4141
Translation	MAEKGASRGTLRRLWSLPRRRRGTAGRSRPDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537484.1

ORF Insert Sequence:

ATGGCCGAGA AGGGAGCATC CCGGGGAACC CTGCGGCGGC TGTGGTCACT GCCCAGGAGG 
CGCCGGGGAA CTGCAGGGCG CTCGAGGCCA GATGAAATCA TGCACCAGGA CATCGTCCCG 
CTCTGTGCTG CCGACATCCA GGACCAGCTA AAGAAGCGCT TTGCTTACCT GTCCGGTGGG 
CGGGGGCAGG ACGGAAGCCC GGTTATCACC TTCCCTGACT ACCCGGCCTT CAGCGAGATT 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TACCTCACCA GCATCCCCAG CCTGCAGGAC 
GCTGGCATCG GATTCATCCT GGTGATAGAC CGGCGACGGG ACAAATGGAC CTCCGTGAAG 
GCGTCCGTCC TGCGCATCGC AGCATCTTTC CCGGCAAACC TGCAGCTCGT CCTCGTGCTT 
CGCCCGACGG GTTTTTTCCA AAGGACTCTC TCCGACATCG CTTTCAAATT CAATAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATAATG CTGAGCTCCG TACCAGACTT ACACGGTTAC 
ATCGATAAGT CGCAGCTGAC CGAGGACCTG GGTGGGACCC TGGACTACTG CCACTCCCGG 
TGGCTGTGCC AGCGCACGGC CATCGAAAGT TTCGCCCTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC CGAGCTGGCT GAAACAGAGC TGCCCAATGA CGTCCAGTCG 
ACAAGCTCAG TGCTGTGTGC GCACACAGAG AAGAAGGACA AGGCGAAGGA GGATTTGAGG 
CTGGCACTGA AAGAGGGGCA CAGTGTCCTG GAGAGCCTCA GGGAGCTGCA GGCTGAGGGC 
TCAGAGCCCA GTGTGAACCA GGACCAGCTT GACAACCAGG CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ACGAAACCGA GGCTGCCTTC GATGAGTTCT GGGCAAAGCA TCAGCAGAAA 
CTGGAGCAGT GTCTGCAGCT CCGGCACTTT GAGCAGGGCT TCCGGGAGGT CAAAGCCATC 
TTGGACGCAG CGTCCCAGAA GATAGCAACC TTCACAGACA TCGGCAACAG CCTGGCGCAT 
GTGGAGCACC TGCTGAGGGA CCTGGCCAGC TTCGAGGAGA AATCAGGCGT GGCCGTGGAG 
AGGGCCCGGG CCCTGTCTCT GGACGGCGAG CAGCTCATTG GGAACAAGCA CTACGCGGTA 
GACTCCATCC GCCCAAAGTG CCAGGAGCTC CGGCACCTCT GTGACCAGTT CTCTGCGGAG 
ATCGCAAGGA GGAGGGGGCT GCTCAGCAAG TCCCTGGAGC TGCACCGCCG CCTGGAGACG 
TCCATGAAGT GGTGTGATGA AGGGATTTAC CTGCTGGCCT CACAACCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCGGA GGCTGCCCTC CAGGAAATCG AGAAGTTTTT GGAGACCGGT 
GCGGAAAATA AGATCCAGGA GCTCAACGCG ATTTACAAGG AATACGAATC CATCCTCAAC 
CAAGATCTCA TGGAGCACGT GCGAAAGGTC TTCCAGAAGC AGGCAAGCAT GGAGGAGGTG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA GGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCAGACCCGA GGCACTGGCA AAGTCGCCCT GCCCCTCCCC AGGCATTCGG 
CGAGGCTCTG AGAACTCCAG CTCCGAGGGC GGTGCGCTCC GGAGAGGGCC CTACCGGAGG 
GCCAAGAGTG AGATGAGTGA GAGCCGGCAG GGCCGCGGCT CAGCGGGGGA GGAGGAGGAA 
AGCCTGGCCA TCCTGCGCAG GCACGTGATG AGCGAGCTCC TGGACACAGA ACGGGCCTAC 
GTGGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCCGCGG AGATGGATAA CCCACTGATG 
GCTCACCTCC TGTCAACAGG CCTTCACAAC AAGAAGGATG TTTTGTTTGG AAACATGGAG 
GAAATCTATC ACTTCCACAA CAGGATATTC CTCAGGGAGC TGGAAAACTA CACTGACTGC 
CCAGAACTGG TTGGAAGATG CTTTCTGGAG AGGATGGAAG ATTTCCAGAT CTATGAGAAG 
TACTGTCAGA ACAAGCCCCG CTCTGAGAGC CTGTGGAGAC AGTGCTCCGA CTGCCCGTTT 
TTCCAGGAAT GCCAGAGAAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTGCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAGG 
AACTGCGAGG GGGCTGAGGA CCTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTGAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCT ATCACCGGCT ATGACGGGAA TCTCGGCGAC 
CTGGGCAAGC TGCTGATGCA GGGCTCGTTC AGCGTCTGGA CCGACCACAA GAGGGGCCAC 
ACCAAGGTGA AGGAGCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCAGTGC TCTTCTGCAA GAAGAGGGAG GAGAATGGGG AGGGGTATGA GAAAGCTCCC 
TCCTACAGCT ACAAGCAGTC CTTAAACATG GCTGCCGTTG GCATTACGGA GAACGTGAAG 
GGAGATGCTA AGAAGTTCGA GATCTGGTAC AACGCGCGCG AGGAGGTCTA CATCGTCCAG 
GCGCCAACTC CTGAGATTAA AGCCGCGTGG GTGAATGAAA TTCGGAAAGT GCTGACCAGC 
CAGCTGCAGG CTTGTAGAGA AGCCAGCCAG CACCGGGCGC TGGAGCAGTC ACAGAGCCTG 
CCCCTGCCGG CCCCGACCAG CACCAGTCCC TCAAGAGGAA ACTCAAGGAA CATCAAGAAG 
CTGGAAGAAA GGAAAACAGA CCCCCTAAGC CTGGAGGGAT ACGTCAGCTC AGCGCCACTG 
ACAAAGCCCC CCGAAAAGGG CAAAGATGAC ACGGTCACTA GCTCTGCCTC AGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCTACCA GTCCTGACAA AAAAGCCAAG CGGCATGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTGTGC GAGGTTGGAG CAAAACGTCC CACTCACTGG AGGCACCTGA GGACGACGGG 
GGCTGGTCAA GTGCAGAGGA GCAGATTAAC TCGTCCGACG CAGAGGAGGA CGGCGGGTTG 
GGCCCCAAGA AGCTGGTTCC AGGTAAATAC ACGGTCGTGG CGGACCACGA GAAGGGAGGC 
CCCGATGCGC TGCGCGTGAG GAGCGGGGAC GTGGTGGAGC TGGTGCAGGA GGGCGACGAG 
GGCCTCTGGT ACGTCAGGGA CCCGACCACT GGCAAGGAGG GCTGGGTGCC GGCCAGCAGC 
CTGTCCGTCC GGCTCGGCCC GTCCGGCTCG GCCCAGTGCC TGAGCAGCTC AGAGTCGAGC 
CCGGGGTCGG CCGTGCTGAG CAACTCGTCC AGCTGCAGCG AGGGCGGCCA GGCCCCCTTC 
TCCGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535787.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu65834
Clone ID Related Accession (Same CDS sequence)	XM_011537485.1
Accession Version	XM_011537485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGATGACAC GGTCACTAGC TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCTACCAGT 
CCTGACAAAA AAGCCAAGCG GCATGAAGTA AAGAGCGACC CGACTCCCTT TGGTGTGCGA 
GGTTGGAGCA AAACGTCCCA CTCACTGGAG GCACCTGAGG ACGACGGGGG CTGGTCAAGT 
GCAGAGGAGC AGATTAACTC GTCCGACGCA GAGGAGGACG GCGGGTTGGG CCCCAAGAAG 
CTGGTTCCAG GTAAATACAC GGTCGTGGCG GACCACGAGA AGGGAGGCCC CGATGCGCTG 
CGCGTGAGGA GCGGGGACGT GGTGGAGCTG GTGCAGGAGG GCGACGAGGG CCTCTGGTAC 
GTCAGGGACC CGACCACTGG CAAGGAGGGC TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG 
CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC 
GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG GGCGGCCAGG CCCCCTTCTC CGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535787.1
CDS	268..3813
Translation	MRFWLRTEEMALEEMVQRLNAVSKHTDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537485.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537485.1

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGGAC TGAAGAGATG GCCTTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCA AGCACACGGA TGAAATCATG CACCAGGACA TCGTCCCGCT CTGTGCTGCC 
GACATCCAGG ACCAGCTAAA GAAGCGCTTT GCTTACCTGT CCGGTGGGCG GGGGCAGGAC 
GGAAGCCCGG TTATCACCTT CCCTGACTAC CCGGCCTTCA GCGAGATTCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA CCTCACCAGC ATCCCCAGCC TGCAGGACGC TGGCATCGGA 
TTCATCCTGG TGATAGACCG GCGACGGGAC AAATGGACCT CCGTGAAGGC GTCCGTCCTG 
CGCATCGCAG CATCTTTCCC GGCAAACCTG CAGCTCGTCC TCGTGCTTCG CCCGACGGGT 
TTTTTCCAAA GGACTCTCTC CGACATCGCT TTCAAATTCA ATAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATAATGCT GAGCTCCGTA CCAGACTTAC ACGGTTACAT CGATAAGTCG 
CAGCTGACCG AGGACCTGGG TGGGACCCTG GACTACTGCC ACTCCCGGTG GCTGTGCCAG 
CGCACGGCCA TCGAAAGTTT CGCCCTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACCG AGCTGGCTGA AACAGAGCTG CCCAATGACG TCCAGTCGAC AAGCTCAGTG 
CTGTGTGCGC ACACAGAGAA GAAGGACAAG GCGAAGGAGG ATTTGAGGCT GGCACTGAAA 
GAGGGGCACA GTGTCCTGGA GAGCCTCAGG GAGCTGCAGG CTGAGGGCTC AGAGCCCAGT 
GTGAACCAGG ACCAGCTTGA CAACCAGGCC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAC 
GAAACCGAGG CTGCCTTCGA TGAGTTCTGG GCAAAGCATC AGCAGAAACT GGAGCAGTGT 
CTGCAGCTCC GGCACTTTGA GCAGGGCTTC CGGGAGGTCA AAGCCATCTT GGACGCAGCG 
TCCCAGAAGA TAGCAACCTT CACAGACATC GGCAACAGCC TGGCGCATGT GGAGCACCTG 
CTGAGGGACC TGGCCAGCTT CGAGGAGAAA TCAGGCGTGG CCGTGGAGAG GGCCCGGGCC 
CTGTCTCTGG ACGGCGAGCA GCTCATTGGG AACAAGCACT ACGCGGTAGA CTCCATCCGC 
CCAAAGTGCC AGGAGCTCCG GCACCTCTGT GACCAGTTCT CTGCGGAGAT CGCAAGGAGG 
AGGGGGCTGC TCAGCAAGTC CCTGGAGCTG CACCGCCGCC TGGAGACGTC CATGAAGTGG 
TGTGATGAAG GGATTTACCT GCTGGCCTCA CAACCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCGGAGG CTGCCCTCCA GGAAATCGAG AAGTTTTTGG AGACCGGTGC GGAAAATAAG 
ATCCAGGAGC TCAACGCGAT TTACAAGGAA TACGAATCCA TCCTCAACCA AGATCTCATG 
GAGCACGTGC GAAAGGTCTT CCAGAAGCAG GCAAGCATGG AGGAGGTGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAGG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
AGACCCGAGG CACTGGCAAA GTCGCCCTGC CCCTCCCCAG GCATTCGGCG AGGCTCTGAG 
AACTCCAGCT CCGAGGGCGG TGCGCTCCGG AGAGGGCCCT ACCGGAGGGC CAAGAGTGAG 
ATGAGTGAGA GCCGGCAGGG CCGCGGCTCA GCGGGGGAGG AGGAGGAAAG CCTGGCCATC 
CTGCGCAGGC ACGTGATGAG CGAGCTCCTG GACACAGAAC GGGCCTACGT GGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCCGCGGAG ATGGATAACC CACTGATGGC TCACCTCCTG 
TCAACAGGCC TTCACAACAA GAAGGATGTT TTGTTTGGAA ACATGGAGGA AATCTATCAC 
TTCCACAACA GGATATTCCT CAGGGAGCTG GAAAACTACA CTGACTGCCC AGAACTGGTT 
GGAAGATGCT TTCTGGAGAG GATGGAAGAT TTCCAGATCT ATGAGAAGTA CTGTCAGAAC 
AAGCCCCGCT CTGAGAGCCT GTGGAGACAG TGCTCCGACT GCCCGTTTTT CCAGGAATGC 
CAGAGAAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTGCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAGGAA CTGCGAGGGG 
GCTGAGGACC TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTGAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCTAT CACCGGCTAT GACGGGAATC TCGGCGACCT GGGCAAGCTG 
CTGATGCAGG GCTCGTTCAG CGTCTGGACC GACCACAAGA GGGGCCACAC CAAGGTGAAG 
GAGCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCAGTGCTC 
TTCTGCAAGA AGAGGGAGGA GAATGGGGAG GGGTATGAGA AAGCTCCCTC CTACAGCTAC 
AAGCAGTCCT TAAACATGGC TGCCGTTGGC ATTACGGAGA ACGTGAAGGG AGATGCTAAG 
AAGTTCGAGA TCTGGTACAA CGCGCGCGAG GAGGTCTACA TCGTCCAGGC GCCAACTCCT 
GAGATTAAAG CCGCGTGGGT GAATGAAATT CGGAAAGTGC TGACCAGCCA GCTGCAGGCT 
TGTAGAGAAG CCAGCCAGCA CCGGGCGCTG GAGCAGTCAC AGAGCCTGCC CCTGCCGGCC 
CCGACCAGCA CCAGTCCCTC AAGAGGAAAC TCAAGGAACA TCAAGAAGCT GGAAGAAAGG 
AAAACAGACC CCCTAAGCCT GGAGGGATAC GTCAGCTCAG CGCCACTGAC AAAGCCCCCC 
GAAAAGGGCA AAGATGACAC GGTCACTAGC TCTGCCTCAG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCTACCAGT 
CCTGACAAAA AAGCCAAGCG GCATGAAGTA AAGAGCGACC CGACTCCCTT TGGTGTGCGA 
GGTTGGAGCA AAACGTCCCA CTCACTGGAG GCACCTGAGG ACGACGGGGG CTGGTCAAGT 
GCAGAGGAGC AGATTAACTC GTCCGACGCA GAGGAGGACG GCGGGTTGGG CCCCAAGAAG 
CTGGTTCCAG GTAAATACAC GGTCGTGGCG GACCACGAGA AGGGAGGCCC CGATGCGCTG 
CGCGTGAGGA GCGGGGACGT GGTGGAGCTG GTGCAGGAGG GCGACGAGGG CCTCTGGTAC 
GTCAGGGACC CGACCACTGG CAAGGAGGGC TGGGTGCCGG CCAGCAGCCT GTCCGTCCGG 
CTCGGCCCGT CCGGCTCGGC CCAGTGCCTG AGCAGCTCAG AGTCGAGCCC GGGGTCGGCC 
GTGCTGAGCA ACTCGTCCAG CTGCAGCGAG GGCGGCCAGG CCCCCTTCTC CGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537487.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535789.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.

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CloneID	OHu65836
Clone ID Related Accession (Same CDS sequence)	XM_011537487.1
Accession Version	XM_011537487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGATG ACACGGTCAC TAGCTCTGCC TCAGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCTAC CAGTCCTGAC 
AAAAAAGCCA AGCGGCATGA AGTAAAGAGC GACCCGACTC CCTTTGGTGT GCGAGGTTGG 
AGCAAAACGT CCCACTCACT GGAGGCACCT GAGGACGACG GGGGCTGGTC AAGTGCAGAG 
GAGCAGATTA ACTCGTCCGA CGCAGAGGAG GACGGCGGGT TGGGCCCCAA GAAGCTGGTT 
CCAGGTAAAT ACACGGTCGT GGCGGACCAC GAGAAGGGAG GCCCCGATGC GCTGCGCGTG 
AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG GAGGGCGACG AGGGCCTCTG GTACGTCAGG 
GACCCGACCA CTGGCAAGGA GGGCTGGGTG CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC 
CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG 
AGCAACTCGT CCAGCTGCAG CGAGGGCGGC CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535789.1
CDS	195..3734
Translation	MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537487.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537487.1

ORF Insert Sequence:

ATGACGGTGC GCCGGCTGTC ACTGCTGTGC CGGGACCTCT GGGCGCTGTG GCTGCTGCTG 
AAGGCCGGCG CAGATGAAAT CATGCACCAG GACATCGTCC CGCTCTGTGC TGCCGACATC 
CAGGACCAGC TAAAGAAGCG CTTTGCTTAC CTGTCCGGTG GGCGGGGGCA GGACGGAAGC 
CCGGTTATCA CCTTCCCTGA CTACCCGGCC TTCAGCGAGA TTCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTACCTCAC CAGCATCCCC AGCCTGCAGG ACGCTGGCAT CGGATTCATC 
CTGGTGATAG ACCGGCGACG GGACAAATGG ACCTCCGTGA AGGCGTCCGT CCTGCGCATC 
GCAGCATCTT TCCCGGCAAA CCTGCAGCTC GTCCTCGTGC TTCGCCCGAC GGGTTTTTTC 
CAAAGGACTC TCTCCGACAT CGCTTTCAAA TTCAATAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATAA TGCTGAGCTC CGTACCAGAC TTACACGGTT ACATCGATAA GTCGCAGCTG 
ACCGAGGACC TGGGTGGGAC CCTGGACTAC TGCCACTCCC GGTGGCTGTG CCAGCGCACG 
GCCATCGAAA GTTTCGCCCT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACCGAGCTGG CTGAAACAGA GCTGCCCAAT GACGTCCAGT CGACAAGCTC AGTGCTGTGT 
GCGCACACAG AGAAGAAGGA CAAGGCGAAG GAGGATTTGA GGCTGGCACT GAAAGAGGGG 
CACAGTGTCC TGGAGAGCCT CAGGGAGCTG CAGGCTGAGG GCTCAGAGCC CAGTGTGAAC 
CAGGACCAGC TTGACAACCA GGCCACCGTG CAGAGGCTCC TGGCCCAGCT GAACGAAACC 
GAGGCTGCCT TCGATGAGTT CTGGGCAAAG CATCAGCAGA AACTGGAGCA GTGTCTGCAG 
CTCCGGCACT TTGAGCAGGG CTTCCGGGAG GTCAAAGCCA TCTTGGACGC AGCGTCCCAG 
AAGATAGCAA CCTTCACAGA CATCGGCAAC AGCCTGGCGC ATGTGGAGCA CCTGCTGAGG 
GACCTGGCCA GCTTCGAGGA GAAATCAGGC GTGGCCGTGG AGAGGGCCCG GGCCCTGTCT 
CTGGACGGCG AGCAGCTCAT TGGGAACAAG CACTACGCGG TAGACTCCAT CCGCCCAAAG 
TGCCAGGAGC TCCGGCACCT CTGTGACCAG TTCTCTGCGG AGATCGCAAG GAGGAGGGGG 
CTGCTCAGCA AGTCCCTGGA GCTGCACCGC CGCCTGGAGA CGTCCATGAA GTGGTGTGAT 
GAAGGGATTT ACCTGCTGGC CTCACAACCT GTGGACAAGT GCCAGTCCCA GGACGGCGCG 
GAGGCTGCCC TCCAGGAAAT CGAGAAGTTT TTGGAGACCG GTGCGGAAAA TAAGATCCAG 
GAGCTCAACG CGATTTACAA GGAATACGAA TCCATCCTCA ACCAAGATCT CATGGAGCAC 
GTGCGAAAGG TCTTCCAGAA GCAGGCAAGC ATGGAGGAGG TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAGGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCAGACCC 
GAGGCACTGG CAAAGTCGCC CTGCCCCTCC CCAGGCATTC GGCGAGGCTC TGAGAACTCC 
AGCTCCGAGG GCGGTGCGCT CCGGAGAGGG CCCTACCGGA GGGCCAAGAG TGAGATGAGT 
GAGAGCCGGC AGGGCCGCGG CTCAGCGGGG GAGGAGGAGG AAAGCCTGGC CATCCTGCGC 
AGGCACGTGA TGAGCGAGCT CCTGGACACA GAACGGGCCT ACGTGGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCCGC GGAGATGGAT AACCCACTGA TGGCTCACCT CCTGTCAACA 
GGCCTTCACA ACAAGAAGGA TGTTTTGTTT GGAAACATGG AGGAAATCTA TCACTTCCAC 
AACAGGATAT TCCTCAGGGA GCTGGAAAAC TACACTGACT GCCCAGAACT GGTTGGAAGA 
TGCTTTCTGG AGAGGATGGA AGATTTCCAG ATCTATGAGA AGTACTGTCA GAACAAGCCC 
CGCTCTGAGA GCCTGTGGAG ACAGTGCTCC GACTGCCCGT TTTTCCAGGA ATGCCAGAGA 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTGCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA GGAACTGCGA GGGGGCTGAG 
GACCTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTGA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CTATCACCGG CTATGACGGG AATCTCGGCG ACCTGGGCAA GCTGCTGATG 
CAGGGCTCGT TCAGCGTCTG GACCGACCAC AAGAGGGGCC ACACCAAGGT GAAGGAGCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCAGT GCTCTTCTGC 
AAGAAGAGGG AGGAGAATGG GGAGGGGTAT GAGAAAGCTC CCTCCTACAG CTACAAGCAG 
TCCTTAAACA TGGCTGCCGT TGGCATTACG GAGAACGTGA AGGGAGATGC TAAGAAGTTC 
GAGATCTGGT ACAACGCGCG CGAGGAGGTC TACATCGTCC AGGCGCCAAC TCCTGAGATT 
AAAGCCGCGT GGGTGAATGA AATTCGGAAA GTGCTGACCA GCCAGCTGCA GGCTTGTAGA 
GAAGCCAGCC AGCACCGGGC GCTGGAGCAG TCACAGAGCC TGCCCCTGCC GGCCCCGACC 
AGCACCAGTC CCTCAAGAGG AAACTCAAGG AACATCAAGA AGCTGGAAGA AAGGAAAACA 
GACCCCCTAA GCCTGGAGGG ATACGTCAGC TCAGCGCCAC TGACAAAGCC CCCCGAAAAG 
GGCAAAGATG ACACGGTCAC TAGCTCTGCC TCAGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCTAC CAGTCCTGAC 
AAAAAAGCCA AGCGGCATGA AGTAAAGAGC GACCCGACTC CCTTTGGTGT GCGAGGTTGG 
AGCAAAACGT CCCACTCACT GGAGGCACCT GAGGACGACG GGGGCTGGTC AAGTGCAGAG 
GAGCAGATTA ACTCGTCCGA CGCAGAGGAG GACGGCGGGT TGGGCCCCAA GAAGCTGGTT 
CCAGGTAAAT ACACGGTCGT GGCGGACCAC GAGAAGGGAG GCCCCGATGC GCTGCGCGTG 
AGGAGCGGGG ACGTGGTGGA GCTGGTGCAG GAGGGCGACG AGGGCCTCTG GTACGTCAGG 
GACCCGACCA CTGGCAAGGA GGGCTGGGTG CCGGCCAGCA GCCTGTCCGT CCGGCTCGGC 
CCGTCCGGCT CGGCCCAGTG CCTGAGCAGC TCAGAGTCGA GCCCGGGGTC GGCCGTGCTG 
AGCAACTCGT CCAGCTGCAG CGAGGGCGGC CAGGCCCCCT TCTCCGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535790.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

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CloneID	OHu65837
Clone ID Related Accession (Same CDS sequence)	XM_011537488.1
Accession Version	XM_011537488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGAA ATGCGTCTGA TTTTTATGAA ATCATGCACC AGGACATCGT CCCGCTCTGT 
GCTGCCGACA TCCAGGACCA GCTAAAGAAG CGCTTTGCTT ACCTGTCCGG TGGGCGGGGG 
CAGGACGGAA GCCCGGTTAT CACCTTCCCT GACTACCCGG CCTTCAGCGA GATTCCGGAC 
AAGGAGTTCC AGAATGTCAT GACCTACCTC ACCAGCATCC CCAGCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGA TGACACGGTC ACTAGCTCTG CCTCAGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTTACCCT GCAGGGTTTT GCTAACCTCA AAGGTCAGAA AGCTTCTCCT 
ACCAGTCCTG ACAAAAAAGC CAAGCGGCAT GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
GTGCGAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535790.1
CDS	82..3573
Translation	MWGNASDFYEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537488.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537488.1

ORF Insert Sequence:

ATGTGGGGAA ATGCGTCTGA TTTTTATGAA ATCATGCACC AGGACATCGT CCCGCTCTGT 
GCTGCCGACA TCCAGGACCA GCTAAAGAAG CGCTTTGCTT ACCTGTCCGG TGGGCGGGGG 
CAGGACGGAA GCCCGGTTAT CACCTTCCCT GACTACCCGG CCTTCAGCGA GATTCCGGAC 
AAGGAGTTCC AGAATGTCAT GACCTACCTC ACCAGCATCC CCAGCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGA TGACACGGTC ACTAGCTCTG CCTCAGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTTACCCT GCAGGGTTTT GCTAACCTCA AAGGTCAGAA AGCTTCTCCT 
ACCAGTCCTG ACAAAAAAGC CAAGCGGCAT GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
GTGCGAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537490.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535792.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu65839
Clone ID Related Accession (Same CDS sequence)	XM_011537490.1
Accession Version	XM_011537490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGATGA CACGGTCACT 
AGCTCTGCCT CAGAAAGCTC TGCGCTTTCC AGAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535792.1
CDS	649..4107
Translation	MHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537490.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537490.1

ORF Insert Sequence:

ATGCACCAGG ACATCGTCCC GCTCTGTGCT GCCGACATCC AGGACCAGCT AAAGAAGCGC 
TTTGCTTACC TGTCCGGTGG GCGGGGGCAG GACGGAAGCC CGGTTATCAC CTTCCCTGAC 
TACCCGGCCT TCAGCGAGAT TCCGGACAAG GAGTTCCAGA ATGTCATGAC CTACCTCACC 
AGCATCCCCA GCCTGCAGGA CGCTGGCATC GGATTCATCC TGGTGATAGA CCGGCGACGG 
GACAAATGGA CCTCCGTGAA GGCGTCCGTC CTGCGCATCG CAGCATCTTT CCCGGCAAAC 
CTGCAGCTCG TCCTCGTGCT TCGCCCGACG GGTTTTTTCC AAAGGACTCT CTCCGACATC 
GCTTTCAAAT TCAATAGAGA TGACTTTAAG ATGAAGGTGC CGGTCATAAT GCTGAGCTCC 
GTACCAGACT TACACGGTTA CATCGATAAG TCGCAGCTGA CCGAGGACCT GGGTGGGACC 
CTGGACTACT GCCACTCCCG GTGGCTGTGC CAGCGCACGG CCATCGAAAG TTTCGCCCTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CCGAGCTGGC TGAAACAGAG 
CTGCCCAATG ACGTCCAGTC GACAAGCTCA GTGCTGTGTG CGCACACAGA GAAGAAGGAC 
AAGGCGAAGG AGGATTTGAG GCTGGCACTG AAAGAGGGGC ACAGTGTCCT GGAGAGCCTC 
AGGGAGCTGC AGGCTGAGGG CTCAGAGCCC AGTGTGAACC AGGACCAGCT TGACAACCAG 
GCCACCGTGC AGAGGCTCCT GGCCCAGCTG AACGAAACCG AGGCTGCCTT CGATGAGTTC 
TGGGCAAAGC ATCAGCAGAA ACTGGAGCAG TGTCTGCAGC TCCGGCACTT TGAGCAGGGC 
TTCCGGGAGG TCAAAGCCAT CTTGGACGCA GCGTCCCAGA AGATAGCAAC CTTCACAGAC 
ATCGGCAACA GCCTGGCGCA TGTGGAGCAC CTGCTGAGGG ACCTGGCCAG CTTCGAGGAG 
AAATCAGGCG TGGCCGTGGA GAGGGCCCGG GCCCTGTCTC TGGACGGCGA GCAGCTCATT 
GGGAACAAGC ACTACGCGGT AGACTCCATC CGCCCAAAGT GCCAGGAGCT CCGGCACCTC 
TGTGACCAGT TCTCTGCGGA GATCGCAAGG AGGAGGGGGC TGCTCAGCAA GTCCCTGGAG 
CTGCACCGCC GCCTGGAGAC GTCCATGAAG TGGTGTGATG AAGGGATTTA CCTGCTGGCC 
TCACAACCTG TGGACAAGTG CCAGTCCCAG GACGGCGCGG AGGCTGCCCT CCAGGAAATC 
GAGAAGTTTT TGGAGACCGG TGCGGAAAAT AAGATCCAGG AGCTCAACGC GATTTACAAG 
GAATACGAAT CCATCCTCAA CCAAGATCTC ATGGAGCACG TGCGAAAGGT CTTCCAGAAG 
CAGGCAAGCA TGGAGGAGGT GTTCCACCGC AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC 
AGGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCAGACCCG AGGCACTGGC AAAGTCGCCC 
TGCCCCTCCC CAGGCATTCG GCGAGGCTCT GAGAACTCCA GCTCCGAGGG CGGTGCGCTC 
CGGAGAGGGC CCTACCGGAG GGCCAAGAGT GAGATGAGTG AGAGCCGGCA GGGCCGCGGC 
TCAGCGGGGG AGGAGGAGGA AAGCCTGGCC ATCCTGCGCA GGCACGTGAT GAGCGAGCTC 
CTGGACACAG AACGGGCCTA CGTGGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCCGCG 
GAGATGGATA ACCCACTGAT GGCTCACCTC CTGTCAACAG GCCTTCACAA CAAGAAGGAT 
GTTTTGTTTG GAAACATGGA GGAAATCTAT CACTTCCACA ACAGGATATT CCTCAGGGAG 
CTGGAAAACT ACACTGACTG CCCAGAACTG GTTGGAAGAT GCTTTCTGGA GAGGATGGAA 
GATTTCCAGA TCTATGAGAA GTACTGTCAG AACAAGCCCC GCTCTGAGAG CCTGTGGAGA 
CAGTGCTCCG ACTGCCCGTT TTTCCAGGAA TGCCAGAGAA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTGCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAG GAACTGCGAG GGGGCTGAGG ACCTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTGAA GGCCGTGAAC GACTCCATGC ACCTCATCGC TATCACCGGC 
TATGACGGGA ATCTCGGCGA CCTGGGCAAG CTGCTGATGC AGGGCTCGTT CAGCGTCTGG 
ACCGACCACA AGAGGGGCCA CACCAAGGTG AAGGAGCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCAGTG CTCTTCTGCA AGAAGAGGGA GGAGAATGGG 
GAGGGGTATG AGAAAGCTCC CTCCTACAGC TACAAGCAGT CCTTAAACAT GGCTGCCGTT 
GGCATTACGG AGAACGTGAA GGGAGATGCT AAGAAGTTCG AGATCTGGTA CAACGCGCGC 
GAGGAGGTCT ACATCGTCCA GGCGCCAACT CCTGAGATTA AAGCCGCGTG GGTGAATGAA 
ATTCGGAAAG TGCTGACCAG CCAGCTGCAG GCTTGTAGAG AAGCCAGCCA GCACCGGGCG 
CTGGAGCAGT CACAGAGCCT GCCCCTGCCG GCCCCGACCA GCACCAGTCC CTCAAGAGGA 
AACTCAAGGA ACATCAAGAA GCTGGAAGAA AGGAAAACAG ACCCCCTAAG CCTGGAGGGA 
TACGTCAGCT CAGCGCCACT GACAAAGCCC CCCGAAAAGG GCAAAGATGA CACGGTCACT 
AGCTCTGCCT CAGAAAGCTC TGCGCTTTCC AGAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCCAA GCGGCATGAA 
GTAAAGAGCG ACCCGACTCC CTTTGGTGTG CGAGGTTGGA GCAAAACGTC CCACTCACTG 
GAGGCACCTG AGGACGACGG GGGCTGGTCA AGTGCAGAGG AGCAGATTAA CTCGTCCGAC 
GCAGAGGAGG ACGGCGGGTT GGGCCCCAAG AAGCTGGTTC CAGGTAAATA CACGGTCGTG 
GCGGACCACG AGAAGGGAGG CCCCGATGCG CTGCGCGTGA GGAGCGGGGA CGTGGTGGAG 
CTGGTGCAGG AGGGCGACGA GGGCCTCTGG TACGTCAGGG ACCCGACCAC TGGCAAGGAG 
GGCTGGGTGC CGGCCAGCAG CCTGTCCGTC CGGCTCGGCC CGTCCGGCTC GGCCCAGTGC 
CTGAGCAGCT CAGAGTCGAG CCCGGGGTCG GCCGTGCTGA GCAACTCGTC CAGCTGCAGC 
GAGGGCGGCC AGGCCCCCTT CTCCGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_011537491.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535793.1 Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.

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CloneID	OHu65840
Clone ID Related Accession (Same CDS sequence)	XM_011537491.1
Accession Version	XM_011537491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange factor DBS isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGA TGACACGGTC ACTAGCTCTG CCTCAGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTTACCCT GCAGGGTTTT GCTAACCTCA AAGGTCAGAA AGCTTCTCCT 
ACCAGTCCTG ACAAAAAAGC CAAGCGGCAT GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
GTGCGAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535793.1
CDS	91..3522
Translation	MWLSQRVLPPLPFPADAWEPSEVLGIFSQHRSHATTSESLTWTSTGTSDARTGVILQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKDDTVTSSASESSALSRKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGVRGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG

Target ORF information:

RefSeq Version	XM_011537491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537491.1

ORF Insert Sequence:

ATGTGGCTGT CGCAGAGAGT GCTGCCTCCT CTCCCGTTTC CAGCAGATGC TTGGGAACCT 
TCAGAAGTCT TGGGGATTTT CTCCCAGCAC CGTAGCCATG CCACCACGAG TGAATCCTTA 
ACGTGGACCA GCACGGGCAC CTCAGATGCC AGGACCGGCG TGATCCTGCA GGACGCTGGC 
ATCGGATTCA TCCTGGTGAT AGACCGGCGA CGGGACAAAT GGACCTCCGT GAAGGCGTCC 
GTCCTGCGCA TCGCAGCATC TTTCCCGGCA AACCTGCAGC TCGTCCTCGT GCTTCGCCCG 
ACGGGTTTTT TCCAAAGGAC TCTCTCCGAC ATCGCTTTCA AATTCAATAG AGATGACTTT 
AAGATGAAGG TGCCGGTCAT AATGCTGAGC TCCGTACCAG ACTTACACGG TTACATCGAT 
AAGTCGCAGC TGACCGAGGA CCTGGGTGGG ACCCTGGACT ACTGCCACTC CCGGTGGCTG 
TGCCAGCGCA CGGCCATCGA AAGTTTCGCC CTCATGGTGA AGCAGACGGC TCAGATGCTG 
CAGTCCTTCG GGACCGAGCT GGCTGAAACA GAGCTGCCCA ATGACGTCCA GTCGACAAGC 
TCAGTGCTGT GTGCGCACAC AGAGAAGAAG GACAAGGCGA AGGAGGATTT GAGGCTGGCA 
CTGAAAGAGG GGCACAGTGT CCTGGAGAGC CTCAGGGAGC TGCAGGCTGA GGGCTCAGAG 
CCCAGTGTGA ACCAGGACCA GCTTGACAAC CAGGCCACCG TGCAGAGGCT CCTGGCCCAG 
CTGAACGAAA CCGAGGCTGC CTTCGATGAG TTCTGGGCAA AGCATCAGCA GAAACTGGAG 
CAGTGTCTGC AGCTCCGGCA CTTTGAGCAG GGCTTCCGGG AGGTCAAAGC CATCTTGGAC 
GCAGCGTCCC AGAAGATAGC AACCTTCACA GACATCGGCA ACAGCCTGGC GCATGTGGAG 
CACCTGCTGA GGGACCTGGC CAGCTTCGAG GAGAAATCAG GCGTGGCCGT GGAGAGGGCC 
CGGGCCCTGT CTCTGGACGG CGAGCAGCTC ATTGGGAACA AGCACTACGC GGTAGACTCC 
ATCCGCCCAA AGTGCCAGGA GCTCCGGCAC CTCTGTGACC AGTTCTCTGC GGAGATCGCA 
AGGAGGAGGG GGCTGCTCAG CAAGTCCCTG GAGCTGCACC GCCGCCTGGA GACGTCCATG 
AAGTGGTGTG ATGAAGGGAT TTACCTGCTG GCCTCACAAC CTGTGGACAA GTGCCAGTCC 
CAGGACGGCG CGGAGGCTGC CCTCCAGGAA ATCGAGAAGT TTTTGGAGAC CGGTGCGGAA 
AATAAGATCC AGGAGCTCAA CGCGATTTAC AAGGAATACG AATCCATCCT CAACCAAGAT 
CTCATGGAGC ACGTGCGAAA GGTCTTCCAG AAGCAGGCAA GCATGGAGGA GGTGTTCCAC 
CGCAGGCAGG CCAGCCTGAA GAAGCTGGCG GCCAGGCAGA CGCGGCCCGT GCAGCCGGTG 
GCCCCCAGAC CCGAGGCACT GGCAAAGTCG CCCTGCCCCT CCCCAGGCAT TCGGCGAGGC 
TCTGAGAACT CCAGCTCCGA GGGCGGTGCG CTCCGGAGAG GGCCCTACCG GAGGGCCAAG 
AGTGAGATGA GTGAGAGCCG GCAGGGCCGC GGCTCAGCGG GGGAGGAGGA GGAAAGCCTG 
GCCATCCTGC GCAGGCACGT GATGAGCGAG CTCCTGGACA CAGAACGGGC CTACGTGGAG 
GAGCTGCTGT GCGTCCTGGA GGGCTACGCC GCGGAGATGG ATAACCCACT GATGGCTCAC 
CTCCTGTCAA CAGGCCTTCA CAACAAGAAG GATGTTTTGT TTGGAAACAT GGAGGAAATC 
TATCACTTCC ACAACAGGAT ATTCCTCAGG GAGCTGGAAA ACTACACTGA CTGCCCAGAA 
CTGGTTGGAA GATGCTTTCT GGAGAGGATG GAAGATTTCC AGATCTATGA GAAGTACTGT 
CAGAACAAGC CCCGCTCTGA GAGCCTGTGG AGACAGTGCT CCGACTGCCC GTTTTTCCAG 
GAATGCCAGA GAAAGCTGGA CCACAAGCTG AGCCTGGACT CCTACCTGCT GAAGCCAGTG 
CAGAGGATCA CCAAGTACCA GCTGCTGCTC AAGGAAATGC TGAAATACAG CAGGAACTGC 
GAGGGGGCTG AGGACCTGCA GGAGGCGCTG AGCTCCATCC TGGGCATCCT GAAGGCCGTG 
AACGACTCCA TGCACCTCAT CGCTATCACC GGCTATGACG GGAATCTCGG CGACCTGGGC 
AAGCTGCTGA TGCAGGGCTC GTTCAGCGTC TGGACCGACC ACAAGAGGGG CCACACCAAG 
GTGAAGGAGC TGGCCAGGTT CAAGCCCATG CAGCGGCACC TGTTCCTGCA CGAGAAGGCA 
GTGCTCTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAAGC TCCCTCCTAC 
AGCTACAAGC AGTCCTTAAA CATGGCTGCC GTTGGCATTA CGGAGAACGT GAAGGGAGAT 
GCTAAGAAGT TCGAGATCTG GTACAACGCG CGCGAGGAGG TCTACATCGT CCAGGCGCCA 
ACTCCTGAGA TTAAAGCCGC GTGGGTGAAT GAAATTCGGA AAGTGCTGAC CAGCCAGCTG 
CAGGCTTGTA GAGAAGCCAG CCAGCACCGG GCGCTGGAGC AGTCACAGAG CCTGCCCCTG 
CCGGCCCCGA CCAGCACCAG TCCCTCAAGA GGAAACTCAA GGAACATCAA GAAGCTGGAA 
GAAAGGAAAA CAGACCCCCT AAGCCTGGAG GGATACGTCA GCTCAGCGCC ACTGACAAAG 
CCCCCCGAAA AGGGCAAAGA TGACACGGTC ACTAGCTCTG CCTCAGAAAG CTCTGCGCTT 
TCCAGAAAGC GCTTTACCCT GCAGGGTTTT GCTAACCTCA AAGGTCAGAA AGCTTCTCCT 
ACCAGTCCTG ACAAAAAAGC CAAGCGGCAT GAAGTAAAGA GCGACCCGAC TCCCTTTGGT 
GTGCGAGGTT GGAGCAAAAC GTCCCACTCA CTGGAGGCAC CTGAGGACGA CGGGGGCTGG 
TCAAGTGCAG AGGAGCAGAT TAACTCGTCC GACGCAGAGG AGGACGGCGG GTTGGGCCCC 
AAGAAGCTGG TTCCAGGTAA ATACACGGTC GTGGCGGACC ACGAGAAGGG AGGCCCCGAT 
GCGCTGCGCG TGAGGAGCGG GGACGTGGTG GAGCTGGTGC AGGAGGGCGA CGAGGGCCTC 
TGGTACGTCA GGGACCCGAC CACTGGCAAG GAGGGCTGGG TGCCGGCCAG CAGCCTGTCC 
GTCCGGCTCG GCCCGTCCGG CTCGGCCCAG TGCCTGAGCA GCTCAGAGTC GAGCCCGGGG 
TCGGCCGTGC TGAGCAACTC GTCCAGCTGC AGCGAGGGCG GCCAGGCCCC CTTCTCCGAC 
CTGCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Establishment of a human primary pancreatic cancer mouse model to examine and investigate gemcitabine resistance.
Oncology reports38(6)3335-3346(2017 Dec)
Zhang YJ,Wen CL,Qin YX,Tang XM,Shi MM,Shen BY,Fang Y

A rare variant in MCF2L identified using exclusion linkage in a pedigree with premature atherosclerosis.
European journal of human genetics : EJHG24(1)86-91(2016 Jan)
Maiwald S,Motazacker MM,van Capelleveen JC,Sivapalaratnam S,van der Wal AC,van der Loos C,Kastelein JJ,Ouwehand WH,Hovingh GK,Trip MD,van Buul JD,Dallinga-Thie GM

Expression analysis of the osteoarthritis genetic susceptibility locus mapping to an intron of the MCF2L gene and marked by the polymorphism rs11842874.
BMC medical genetics16108(2015 Nov)
Shepherd C,Skelton AJ,Rushton MD,Reynard LN,Loughlin J

Regulation of vesicle transport and cell motility by Golgi-localized Dbs.
Small GTPases5(4)1-12(2014)
Fitzpatrick ER,Hu T,Ciccarelli BT,Whitehead IP

Genetic contribution to radiographic severity in osteoarthritis of the knee.
Annals of the rheumatic diseases71(9)1537-40(2012 Sep)
Valdes AM,Doherty S,Muir KR,Zhang W,Maciewicz RA,Wheeler M,Arden N,Cooper C,Doherty M

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MCF2L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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