MAST1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAST1
Entrez Gene ID	22983
Full Name	microtubule associated serine/threonine kinase 1
Synonyms	SAST
General protein information	Preferred Names microtubule associated serine/threonine kinase 1 Names microtubule-associated serine/threonine-protein kinase 1 syntrophin associated serine/threonine kinase syntrophin-associated serine/threonine-protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	This gene is a member of the microtubule-associated serine/threonine kinase (MAST) family. The protein encoded by this gene has an N-terminal serine/threonine kinase domain followed by a postsynaptic density protein-95/discs large/zona occludens-1 (PDZ) domain. In mouse and rat, the orthologous protein associates with the cytoskeleton and can bind both beta-2-syntrophin and neuronal nitric oxide synthase (nNOS) through its PDZ domain. In mouse and rat, this protein also co-localizes with dystrophin- and utrophin-associated protein complexes (DAPC/UAPC) in the vascular endothelium of the central nervous system. [provided by RefSeq, May 2017].
Disorder MIM:	612256

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527805.2	XP_011526107.1	microtubule-associated serine/threonine-protein kinase 1 isoform X1
NM_014975.2	NP_055790.1	microtubule-associated serine/threonine-protein kinase 1
XM_011527808.2	XP_011526110.1	microtubule-associated serine/threonine-protein kinase 1 isoform X2
NM_014975.3	NP_055790.1	microtubule-associated serine/threonine-protein kinase 1
XM_017026501.1	XP_016881990.1	microtubule-associated serine/threonine-protein kinase 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MAST1	NP_001178457.1
Danio rerio (zebrafish)	LOC796144	XP_001336447.3
Xenopus tropicalis (tropical clawed frog)	mast1	XP_002938529.2
Pan troglodytes (chimpanzee)	MAST1	XP_003316199.2
Canis lupus familiaris (dog)	MAST1	XP_005633268.1
Danio rerio (zebrafish)	mast1a	XP_001337128.3
Rattus norvegicus (Norway rat)	MAST1	NP_851603.1
Homo sapiens (human)	MAST1	NP_055790.1
	MAST1	XP_001109914.2
Mus musculus (house mouse)	Mast1	NP_064329.2

Gene Ontology
Bibliography

Related articles in PubMed

Functionally recurrent rearrangements of the MAST kinase and Notch gene families in breast cancer.
Robinson DR, Kalyana-Sundaram S, Wu YM, Shankar S, Cao X, Ateeq B, Asangani IA, Iyer M, Maher CA, Grasso CS, Lonigro RJ, Quist M, Siddiqui J, Mehra R, Jing X, Giordano TJ, Sabel MS, Kleer CG, Palanisamy N, Natrajan R, Lambros MB, Reis-Filho JS, Kumar-Sinha C, Chinnaiyan AM
Nature medicine17(12)1646-51(2011 Nov)

Regulation of epidermal growth factor receptor trafficking by lysine deacetylase HDAC6.
Deribe YL, Wild P, Chandrashaker A, Curak J, Schmidt MH, Kalaidzidis Y, Milutinovic N, Kratchmarova I, Buerkle L, Fetchko MJ, Schmidt P, Kittanakom S, Brown KR, Jurisica I, Blagoev B, Zerial M, Stagljar I, Dikic I
Science signaling2(102)ra84(2009 Dec)

Sast124, a novel splice variant of syntrophin-associated serine/threonine kinase (SAST), is specifically localized in the restricted brain regions.
Yano R, Yap CC, Yamazaki Y, Muto Y, Kishida H, Okada D, Hashikawa T
Neuroscience117(2)373-81(2003)

Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases.
Lumeng C, Phelps S, Crawford GE, Walden PD, Barald K, Chamberlain JS
Nature neuroscience2(7)611-7(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of MAST1 or MAST2 gene fusions has a proliferative effect both in vitro and in vivo in breast cancer cell lines.
Title: Functionally recurrent rearrangements of the MAST kinase and Notch gene families in breast cancer.

Discusses cloning of Sast124, the rat ortholog of MAST1.
Title: Sast124, a novel splice variant of syntrophin-associated serine/threonine kinase (SAST), is specifically localized in the restricted brain regions.

Discusses cloning of Sast, the mouse ortholog of human MAST1.
Title: Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases.

The following MAST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32035	NM_014975.3 Latest version!	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu65209	XM_011527805.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65211	XM_011527808.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu88317	XM_017026501.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu32035	NM_014975.2	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAST1 ( NM_014975.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055790.1 Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.

Price & Availability↑

CloneID	OHu32035
Clone ID Related Accession (Same CDS sequence)	NM_014975.2 , NM_014975.3
Accession Version	NM_014975.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA769770.1, AB023190.1, AC020934.9 and BM681304.1. This sequence is a reference standard in the RefSeqGene project. On Jun 6, 2009 this sequence version replaced NM_014975.1. ##Evidence-Data-START## Transcript exon combination :: AB023190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACT CTCTCTGGAC CGCGCTTTCT AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGTATGTTCC GCCGCACCAA GAGCTGCCGC ACCAGTAATC GGAAAAGCCT CATCCTGACC 
AGCACTTCAC CCACGCTACC GAGACCCCAC TCCCCGCTGC CAGGTCACCT AGGCAGCAGT 
CCCCTGGACA GCCCCCGAAA CTTCTCCCCC AACACCCCCG CCCACTTCTC GTTTGCCTCC 
TCCCGAAGGG CGGACGGACG CCGGTGGTCT CTGGCCTCGC TCCCTTCATC TGGCTATGGC 
ACCAACACGC CCAGTTCCAC CGTCTCGTCC TCCTGCTCCT CCCAGGAGCG CCTTCACCAG 
CTGCCCTACC AGCCCACGGT GGACGAGCTC CACTTCCTCT CCAAACACTT CGGGAGCACC 
GAGAGCATCA CAGACGAGGA TGGTGGCCGT CGCTCCCCAG CCGTGCGGCC CCGCTCACGG 
AGCCTCAGCC CCGGGCGCTC CCCCTCCTCC TACGACAACG AGATCGTGAT GATGAATCAC 
GTCTACAAGG AGAGGTTCCC GAAGGCCACT GCGCAGATGG AGGAGAAGCT GCGCGACTTT 
ACCCGCGCCT ACGAACCCGA CAGCGTTCTG CCTCTGGCCG ATGGCGTGCT CAGCTTCATC 
CACCACCAGA TCATCGAGCT GGCCCGGGAC TGCCTGACCA AGTCCCGTGA CGGCCTCATC 
ACCACGGTCT ACTTCTATGA ATTGCAGGAG AACCTGGAGA AGCTCCTTCA AGACGCCTAT 
GAACGCTCTG AGAGCTTGGA GGTGGCCTTC GTTACTCAGC TGGTGAAGAA GTTGCTTATT 
ATCATCTCAC GCCCTGCGAG GCTGCTGGAG TGCCTGGAAT TCAACCCCGA GGAGTTCTAC 
CACCTGCTGG AGGCGGCCGA AGGACACGCC AAGGAGGGCC ACCTTGTGAA GACGGACATC 
CCCCGCTACA TCATCCGCCA GCTGGGCCTC ACCCGTGACC CCTTTCCAGA TGTGGTGCAT 
CTGGAGGAAC AGGACAGTGG TGGTTCCAAC ACCCCTGAGC AAGACGATCT CTCTGAGGGC 
CGCAGCAGCA AGGCCAAGAA ACCGCCGGGG GAGAATGACT TCGATACCAT CAAGCTCATA 
AGCAACGGTG CCTACGGCGC TGTCTACCTG GTGCGGCACC GCGACACGCG GCAGCGCTTT 
GCCATGAAAA AGATCAACAA GCAGAACTTG ATCCTCCGCA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGCG ATATCCTCAC CTTCGCCGAG AACCCGTTTG TGGTCGGCAT GTTCTGCTCC 
TTTGAGACTC GGCGCCACCT CTGCATGGTC ATGGAATATG TGGAAGGCGG CGACTGTGCC 
ACCCTGCTGA AGAATATTGG AGCGCTGCCC GTAGAGATGG CCCGCATGTA CTTTGCTGAG 
ACGGTGCTAG CCCTGGAGTA TTTGCACAAC TATGGCATCG TGCACCGCGA CCTCAAGCCT 
GACAACCTCC TTATCACCTC CATGGGTCAC ATCAAGCTCA CAGATTTCGG CCTCTCCAAG 
ATGGGGCTCA TGAGCCTCAC CACCAACTTA TATGAAGGCC ACATCGAGAA GGACGCCCGA 
GAGTTCCTGG ACAAACAGGT GTGTGGGACC CCAGAGTACA TCGCGCCCGA GGTCATCCTG 
CGTCAAGGCT ACGGCAAGCC AGTGGACTGG TGGGCTATGG GGATCATCCT CTACGAGTTC 
CTGGTGGGCT GTGTGCCCTT CTTCGGAGAC ACACCAGAGG AGCTATTTGG ACAGGTCATC 
AGTGATGACA TCCTGTGGCC CGAGGGGGAT GAGGCCCTAC CTACGGAGGC CCAACTCCTC 
ATATCCAGCC TCCTGCAGAC CAACCCTCTG GTCAGGCTTG GGGCAGGCGG CGCTTTTGAG 
GTGAAGCAGC ACAGTTTCTT TCGAGACCTG GACTGGACAG GGCTGCTGAG GCAGAAGGCC 
GAGTTCATCC CCCACCTAGA GTCGGAAGAT GACACTAGCT ACTTTGACAC CCGCTCAGAC 
AGGTATCACC ACGTGAACTC CTATGACGAG GATGACACGA CGGAGGAGGA GCCCGTGGAA 
ATCCGCCAGT TCTCTTCCTG CTCTCCGCGC TTCAGCAAGG TGTATAGCAG CATGGAGCAG 
CTGTCGCAGC ACGAGCCCAA GACCCCAGTA GCAGCTGCAG GGAGCAGCAA GCGGGAGCCG 
AGCACCAAGG GCCCCGAGGA GAAGGTGGCC GGCAAGCGGG AGGGGCTGGG CGGCCTGACC 
CTGCGTGAGA AGACCTGGAG AGGGGGCTCT CCGGAGATCA AGCGATTCTC CGCGTCCGAG 
GCCAGTTTCC TGGAGGGAGA GGCCAGTCCC CCTTTGGGCG CCCGCCGCCG TTTCTCGGCG 
CTGCTGGAGC CCAGCCGCTT CAGCGCCCCC CAAGAGGACG AGGATGAGGC CCGGCTGCGC 
AGGCCTCCCC GGCCCAGCTC CGACCCCGCG GGATCCCTGG ATGCACGGGC CCCCAAAGAG 
GAGACTCAAG GGGAAGGCAC CTCCAGCGCC GGGGACTCCG AGGCCACTGA CCGTCCACGC 
CCAGGTGACC TCTGCCCACC CTCGAAGGAT GGGGATGCAT CAGGCCCAAG GGCTACCAAT 
GACTTGGTTC TGCGCCGGGC GCGGCACCAG CAGATGTCAG GGGATGTGGC AGTAGAGAAG 
AGGCCTTCTC GAACTGGGGG CAAAGTCATC AAATCAGCCT CAGCCACTGC CTTATCTGTC 
ATGATTCCTG CAGTGGACCC ACATGGAAGT TCACCCCTTG CTAGTCCCAT GTCTCCACGA 
TCTCTGTCCT CCAACCCATC CTCACGGGAC TCCTCACCCA GCCGGGACTA CTCACCAGCT 
GTCAGTGGGC TCCGCTCCCC CATCACCATC CAGCGCTCGG GCAAGAAGTA TGGCTTCACA 
CTGCGTGCCA TCCGTGTCTA CATGGGTGAC ACGGATGTCT ATAGTGTCCA CCACATTGTC 
TGGCATGTGG AGGAAGGAGG CCCAGCCCAG GAGGCAGGAC TCTGTGCTGG GGACCTCATC 
ACCCACGTGA ATGGGGAGCC TGTGCATGGC ATGGTGCATC CTGAGGTCGT GGAGCTGATC 
CTTAAGAGTG GCAACAAGGT AGCAGTGACC ACAACGCCCT TCGAAAATAC CTCTATCCGC 
ATTGGTCCCG CAAGGCGCAG CAGCTACAAG GCTAAAATGG CTCGGAGGAA CAAGCGACCC 
TCCGCCAAGG AGGGCCAGGA GAGCAAGAAG CGCAGCTCCC TCTTCCGGAA GATCACGAAG 
CAGTCGAACC TGCTGCATAC TAGCCGCTCG CTGTCGTCGC TGAACCGCTC GCTGTCATCC 
AGCGATAGTC TCCCGGGCTC GCCTACGCAC GGGCTGCCGG CGCGCTCGCC CACGCACAGC 
TACCGCTCCA CGCCTGACTC CGCCTACCTA GGCGCCTCAT CCCAGAGCAG CTCCCCAGCC 
TCGAGCACGC CCAACTCGCC TGCGTCGTCG GCGTCGCACC ACATTCGGCC CAGCACGCTG 
CACGGACTGT CGCCAAAGCT CCATCGCCAG TACCGCTCTG CGCGATGCAA GTCGGCCGGC 
AACATCCCTC TATCGCCGCT GGCACACACG CCGTCCCCCA CGCAGGCGTC ACCGCCGCCA 
CTGCCGGGCC ACACGGTGGG CAGCTCGCAC ACTACTCAGA GCTTCCCGGC CAAACTGCAC 
TCATCGCCTC CCGTCGTGCG CCCGCGCCCC AAGAGTGCCG AGCCCCCTCG CTCGCCGCTC 
CTCAAGCGCG TGCAGTCGGC CGAGAAGCTG GGAGCCTCTT TGAGTGCGGA CAAGAAGGGC 
GCGCTGCGCA AACACAGCCT CGAGGTGGGC CACCCGGATT TCCGCAAGGA CTTCCATGGC 
GAGCTGGCGC TGCATAGCCT TGCCGAGTCC GACGGTGAGA CGCCCCCAGT CGAGGGCCTT 
GGCGCGCCCC GGCAGGTCGC CGTCCGCCGC CTGGGCCGAC AGGAGTCACC TTTGAGCCTG 
GGCGCGGACC CGTTGCTGCC CGAGGGTGCC TCCAGGCCAC CAGTGTCGAG CAAGGAGAAG 
GAATCCCCGG GGGGCGCCGA GGCGTGCACC CCACCCCGCG CGACGACCCC CGGTGGCCGG 
ACCCTGGAGC GGGACGTCGG CTGCACGCGG CATCAGAGCG TGCAGACGGA GGATGGCACT 
GGCGGGATGG CCAGGGCTGT GGCCAAGGCG GCGCTGAGCC CGGTGCAGGA ACACGAGACA 
GGCCGGCGCA GCAGCTCTGG CGAGGCGGGC ACACCCCTGG TACCCATTGT CGTAGAGCCT 
GCGCGGCCCG GGGCTAAGGC TGTGGTGCCT CAGCCTCTGG GCGCGGACTC CAAGGGGTTG 
CAGGAACCCG CACCCCTGGC GCCTTCCGTG CCCGAGGCCC CCCGGGGCCG GGAGCGCTGG 
GTGTTGGAGG TGGTGGAGGA GCGCACCACG CTGAGCGGTC CTCGCTCCAA GCCCGCCTCC 
CCAAAGCTCT CCCCGGAGCC CCAGACACCC TCCCTAGCCC CAGCGAAGTG CAGTGCACCC 
AGCAGTGCAG TGACCCCAGT CCCACCCGCA TCCCTCTTGG GCTCAGGCAC CAAGCCTCAA 
GTGGGGCTGA CCTCCCGGTG CCCTGCTGAA GCTGTGCCCC CAGCAGGCCT GACCAAAAAA 
GGAGTGTCCA GTCCCGCACC CCCGGGACCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055790.1
CDS	129..4841
Translation	MSDSLWTALSNFSMPSFPGGSMFRRTKSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFTRAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVHLEEQDSGGSNTPEQDDLSEGRSSKAKKPPGENDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVAAAGSSKREPSTKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFSAPQEDEDEARLRRPPRPSSDPAGSLDARAPKEETQGEGTSSAGDSEATDRPRPGDLCPPSKDGDASGPRATNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLSADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPVEGLGAPRQVAVRRLGRQESPLSLGADPLLPEGASRPPVSSKEKESPGGAEACTPPRATTPGGRTLERDVGCTRHQSVQTEDGTGGMARAVAKAALSPVQEHETGRRSSSGEAGTPLVPIVVEPARPGAKAVVPQPLGADSKGLQEPAPLAPSVPEAPRGRERWVLEVVEERTTLSGPRSKPASPKLSPEPQTPSLAPAKCSAPSSAVTPVPPASLLGSGTKPQVGLTSRCPAEAVPPAGLTKKGVSSPAPPGP

Target ORF information:

RefSeq Version	NM_014975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014975.2

ORF Insert Sequence:

ATGTCTGACT CTCTCTGGAC CGCGCTTTCT AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGTATGTTCC GCCGCACCAA GAGCTGCCGC ACCAGTAATC GGAAAAGCCT CATCCTGACC 
AGCACTTCAC CCACGCTACC GAGACCCCAC TCCCCGCTGC CAGGTCACCT AGGCAGCAGT 
CCCCTGGACA GCCCCCGAAA CTTCTCCCCC AACACCCCCG CCCACTTCTC GTTTGCCTCC 
TCCCGAAGGG CGGACGGACG CCGGTGGTCT CTGGCCTCGC TCCCTTCATC TGGCTATGGC 
ACCAACACGC CCAGTTCCAC CGTCTCGTCC TCCTGCTCCT CCCAGGAGCG CCTTCACCAG 
CTGCCCTACC AGCCCACGGT GGACGAGCTC CACTTCCTCT CCAAACACTT CGGGAGCACC 
GAGAGCATCA CAGACGAGGA TGGTGGCCGT CGCTCCCCAG CCGTGCGGCC CCGCTCACGG 
AGCCTCAGCC CCGGGCGCTC CCCCTCCTCC TACGACAACG AGATCGTGAT GATGAATCAC 
GTCTACAAGG AGAGGTTCCC GAAGGCCACT GCGCAGATGG AGGAGAAGCT GCGCGACTTT 
ACCCGCGCCT ACGAACCCGA CAGCGTTCTG CCTCTGGCCG ATGGCGTGCT CAGCTTCATC 
CACCACCAGA TCATCGAGCT GGCCCGGGAC TGCCTGACCA AGTCCCGTGA CGGCCTCATC 
ACCACGGTCT ACTTCTATGA ATTGCAGGAG AACCTGGAGA AGCTCCTTCA AGACGCCTAT 
GAACGCTCTG AGAGCTTGGA GGTGGCCTTC GTTACTCAGC TGGTGAAGAA GTTGCTTATT 
ATCATCTCAC GCCCTGCGAG GCTGCTGGAG TGCCTGGAAT TCAACCCCGA GGAGTTCTAC 
CACCTGCTGG AGGCGGCCGA AGGACACGCC AAGGAGGGCC ACCTTGTGAA GACGGACATC 
CCCCGCTACA TCATCCGCCA GCTGGGCCTC ACCCGTGACC CCTTTCCAGA TGTGGTGCAT 
CTGGAGGAAC AGGACAGTGG TGGTTCCAAC ACCCCTGAGC AAGACGATCT CTCTGAGGGC 
CGCAGCAGCA AGGCCAAGAA ACCGCCGGGG GAGAATGACT TCGATACCAT CAAGCTCATA 
AGCAACGGTG CCTACGGCGC TGTCTACCTG GTGCGGCACC GCGACACGCG GCAGCGCTTT 
GCCATGAAAA AGATCAACAA GCAGAACTTG ATCCTCCGCA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGCG ATATCCTCAC CTTCGCCGAG AACCCGTTTG TGGTCGGCAT GTTCTGCTCC 
TTTGAGACTC GGCGCCACCT CTGCATGGTC ATGGAATATG TGGAAGGCGG CGACTGTGCC 
ACCCTGCTGA AGAATATTGG AGCGCTGCCC GTAGAGATGG CCCGCATGTA CTTTGCTGAG 
ACGGTGCTAG CCCTGGAGTA TTTGCACAAC TATGGCATCG TGCACCGCGA CCTCAAGCCT 
GACAACCTCC TTATCACCTC CATGGGTCAC ATCAAGCTCA CAGATTTCGG CCTCTCCAAG 
ATGGGGCTCA TGAGCCTCAC CACCAACTTA TATGAAGGCC ACATCGAGAA GGACGCCCGA 
GAGTTCCTGG ACAAACAGGT GTGTGGGACC CCAGAGTACA TCGCGCCCGA GGTCATCCTG 
CGTCAAGGCT ACGGCAAGCC AGTGGACTGG TGGGCTATGG GGATCATCCT CTACGAGTTC 
CTGGTGGGCT GTGTGCCCTT CTTCGGAGAC ACACCAGAGG AGCTATTTGG ACAGGTCATC 
AGTGATGACA TCCTGTGGCC CGAGGGGGAT GAGGCCCTAC CTACGGAGGC CCAACTCCTC 
ATATCCAGCC TCCTGCAGAC CAACCCTCTG GTCAGGCTTG GGGCAGGCGG CGCTTTTGAG 
GTGAAGCAGC ACAGTTTCTT TCGAGACCTG GACTGGACAG GGCTGCTGAG GCAGAAGGCC 
GAGTTCATCC CCCACCTAGA GTCGGAAGAT GACACTAGCT ACTTTGACAC CCGCTCAGAC 
AGGTATCACC ACGTGAACTC CTATGACGAG GATGACACGA CGGAGGAGGA GCCCGTGGAA 
ATCCGCCAGT TCTCTTCCTG CTCTCCGCGC TTCAGCAAGG TGTATAGCAG CATGGAGCAG 
CTGTCGCAGC ACGAGCCCAA GACCCCAGTA GCAGCTGCAG GGAGCAGCAA GCGGGAGCCG 
AGCACCAAGG GCCCCGAGGA GAAGGTGGCC GGCAAGCGGG AGGGGCTGGG CGGCCTGACC 
CTGCGTGAGA AGACCTGGAG AGGGGGCTCT CCGGAGATCA AGCGATTCTC CGCGTCCGAG 
GCCAGTTTCC TGGAGGGAGA GGCCAGTCCC CCTTTGGGCG CCCGCCGCCG TTTCTCGGCG 
CTGCTGGAGC CCAGCCGCTT CAGCGCCCCC CAAGAGGACG AGGATGAGGC CCGGCTGCGC 
AGGCCTCCCC GGCCCAGCTC CGACCCCGCG GGATCCCTGG ATGCACGGGC CCCCAAAGAG 
GAGACTCAAG GGGAAGGCAC CTCCAGCGCC GGGGACTCCG AGGCCACTGA CCGTCCACGC 
CCAGGTGACC TCTGCCCACC CTCGAAGGAT GGGGATGCAT CAGGCCCAAG GGCTACCAAT 
GACTTGGTTC TGCGCCGGGC GCGGCACCAG CAGATGTCAG GGGATGTGGC AGTAGAGAAG 
AGGCCTTCTC GAACTGGGGG CAAAGTCATC AAATCAGCCT CAGCCACTGC CTTATCTGTC 
ATGATTCCTG CAGTGGACCC ACATGGAAGT TCACCCCTTG CTAGTCCCAT GTCTCCACGA 
TCTCTGTCCT CCAACCCATC CTCACGGGAC TCCTCACCCA GCCGGGACTA CTCACCAGCT 
GTCAGTGGGC TCCGCTCCCC CATCACCATC CAGCGCTCGG GCAAGAAGTA TGGCTTCACA 
CTGCGTGCCA TCCGTGTCTA CATGGGTGAC ACGGATGTCT ATAGTGTCCA CCACATTGTC 
TGGCATGTGG AGGAAGGAGG CCCAGCCCAG GAGGCAGGAC TCTGTGCTGG GGACCTCATC 
ACCCACGTGA ATGGGGAGCC TGTGCATGGC ATGGTGCATC CTGAGGTCGT GGAGCTGATC 
CTTAAGAGTG GCAACAAGGT AGCAGTGACC ACAACGCCCT TCGAAAATAC CTCTATCCGC 
ATTGGTCCCG CAAGGCGCAG CAGCTACAAG GCTAAAATGG CTCGGAGGAA CAAGCGACCC 
TCCGCCAAGG AGGGCCAGGA GAGCAAGAAG CGCAGCTCCC TCTTCCGGAA GATCACGAAG 
CAGTCGAACC TGCTGCATAC TAGCCGCTCG CTGTCGTCGC TGAACCGCTC GCTGTCATCC 
AGCGATAGTC TCCCGGGCTC GCCTACGCAC GGGCTGCCGG CGCGCTCGCC CACGCACAGC 
TACCGCTCCA CGCCTGACTC CGCCTACCTA GGCGCCTCAT CCCAGAGCAG CTCCCCAGCC 
TCGAGCACGC CCAACTCGCC TGCGTCGTCG GCGTCGCACC ACATTCGGCC CAGCACGCTG 
CACGGACTGT CGCCAAAGCT CCATCGCCAG TACCGCTCTG CGCGATGCAA GTCGGCCGGC 
AACATCCCTC TATCGCCGCT GGCACACACG CCGTCCCCCA CGCAGGCGTC ACCGCCGCCA 
CTGCCGGGCC ACACGGTGGG CAGCTCGCAC ACTACTCAGA GCTTCCCGGC CAAACTGCAC 
TCATCGCCTC CCGTCGTGCG CCCGCGCCCC AAGAGTGCCG AGCCCCCTCG CTCGCCGCTC 
CTCAAGCGCG TGCAGTCGGC CGAGAAGCTG GGAGCCTCTT TGAGTGCGGA CAAGAAGGGC 
GCGCTGCGCA AACACAGCCT CGAGGTGGGC CACCCGGATT TCCGCAAGGA CTTCCATGGC 
GAGCTGGCGC TGCATAGCCT TGCCGAGTCC GACGGTGAGA CGCCCCCAGT CGAGGGCCTT 
GGCGCGCCCC GGCAGGTCGC CGTCCGCCGC CTGGGCCGAC AGGAGTCACC TTTGAGCCTG 
GGCGCGGACC CGTTGCTGCC CGAGGGTGCC TCCAGGCCAC CAGTGTCGAG CAAGGAGAAG 
GAATCCCCGG GGGGCGCCGA GGCGTGCACC CCACCCCGCG CGACGACCCC CGGTGGCCGG 
ACCCTGGAGC GGGACGTCGG CTGCACGCGG CATCAGAGCG TGCAGACGGA GGATGGCACT 
GGCGGGATGG CCAGGGCTGT GGCCAAGGCG GCGCTGAGCC CGGTGCAGGA ACACGAGACA 
GGCCGGCGCA GCAGCTCTGG CGAGGCGGGC ACACCCCTGG TACCCATTGT CGTAGAGCCT 
GCGCGGCCCG GGGCTAAGGC TGTGGTGCCT CAGCCTCTGG GCGCGGACTC CAAGGGGTTG 
CAGGAACCCG CACCCCTGGC GCCTTCCGTG CCCGAGGCCC CCCGGGGCCG GGAGCGCTGG 
GTGTTGGAGG TGGTGGAGGA GCGCACCACG CTGAGCGGTC CTCGCTCCAA GCCCGCCTCC 
CCAAAGCTCT CCCCGGAGCC CCAGACACCC TCCCTAGCCC CAGCGAAGTG CAGTGCACCC 
AGCAGTGCAG TGACCCCAGT CCCACCCGCA TCCCTCTTGG GCTCAGGCAC CAAGCCTCAA 
GTGGGGCTGA CCTCCCGGTG CCCTGCTGAA GCTGTGCCCC CAGCAGGCCT GACCAAAAAA 
GGAGTGTCCA GTCCCGCACC CCCGGGACCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST1 ( NM_014975.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055790.1 Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.

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CloneID	OHu32035
Clone ID Related Accession (Same CDS sequence)	NM_014975.2 , NM_014975.3
Accession Version	NM_014975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA769770.1, AB023190.1, AC020934.9 and BM681304.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014975.2. ##Evidence-Data-START## Transcript exon combination :: AB023190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000251472.9/ ENSP00000251472.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGACT CTCTCTGGAC CGCGCTTTCT AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGTATGTTCC GCCGCACCAA GAGCTGCCGC ACCAGTAATC GGAAAAGCCT CATCCTGACC 
AGCACTTCAC CCACGCTACC GAGACCCCAC TCCCCGCTGC CAGGTCACCT AGGCAGCAGT 
CCCCTGGACA GCCCCCGAAA CTTCTCCCCC AACACCCCCG CCCACTTCTC GTTTGCCTCC 
TCCCGAAGGG CGGACGGACG CCGGTGGTCT CTGGCCTCGC TCCCTTCATC TGGCTATGGC 
ACCAACACGC CCAGTTCCAC CGTCTCGTCC TCCTGCTCCT CCCAGGAGCG CCTTCACCAG 
CTGCCCTACC AGCCCACGGT GGACGAGCTC CACTTCCTCT CCAAACACTT CGGGAGCACC 
GAGAGCATCA CAGACGAGGA TGGTGGCCGT CGCTCCCCAG CCGTGCGGCC CCGCTCACGG 
AGCCTCAGCC CCGGGCGCTC CCCCTCCTCC TACGACAACG AGATCGTGAT GATGAATCAC 
GTCTACAAGG AGAGGTTCCC GAAGGCCACT GCGCAGATGG AGGAGAAGCT GCGCGACTTT 
ACCCGCGCCT ACGAACCCGA CAGCGTTCTG CCTCTGGCCG ATGGCGTGCT CAGCTTCATC 
CACCACCAGA TCATCGAGCT GGCCCGGGAC TGCCTGACCA AGTCCCGTGA CGGCCTCATC 
ACCACGGTCT ACTTCTATGA ATTGCAGGAG AACCTGGAGA AGCTCCTTCA AGACGCCTAT 
GAACGCTCTG AGAGCTTGGA GGTGGCCTTC GTTACTCAGC TGGTGAAGAA GTTGCTTATT 
ATCATCTCAC GCCCTGCGAG GCTGCTGGAG TGCCTGGAAT TCAACCCCGA GGAGTTCTAC 
CACCTGCTGG AGGCGGCCGA AGGACACGCC AAGGAGGGCC ACCTTGTGAA GACGGACATC 
CCCCGCTACA TCATCCGCCA GCTGGGCCTC ACCCGTGACC CCTTTCCAGA TGTGGTGCAT 
CTGGAGGAAC AGGACAGTGG TGGTTCCAAC ACCCCTGAGC AAGACGATCT CTCTGAGGGC 
CGCAGCAGCA AGGCCAAGAA ACCGCCGGGG GAGAATGACT TCGATACCAT CAAGCTCATA 
AGCAACGGTG CCTACGGCGC TGTCTACCTG GTGCGGCACC GCGACACGCG GCAGCGCTTT 
GCCATGAAAA AGATCAACAA GCAGAACTTG ATCCTCCGCA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGCG ATATCCTCAC CTTCGCCGAG AACCCGTTTG TGGTCGGCAT GTTCTGCTCC 
TTTGAGACTC GGCGCCACCT CTGCATGGTC ATGGAATATG TGGAAGGCGG CGACTGTGCC 
ACCCTGCTGA AGAATATTGG AGCGCTGCCC GTAGAGATGG CCCGCATGTA CTTTGCTGAG 
ACGGTGCTAG CCCTGGAGTA TTTGCACAAC TATGGCATCG TGCACCGCGA CCTCAAGCCT 
GACAACCTCC TTATCACCTC CATGGGTCAC ATCAAGCTCA CAGATTTCGG CCTCTCCAAG 
ATGGGGCTCA TGAGCCTCAC CACCAACTTA TATGAAGGCC ACATCGAGAA GGACGCCCGA 
GAGTTCCTGG ACAAACAGGT GTGTGGGACC CCAGAGTACA TCGCGCCCGA GGTCATCCTG 
CGTCAAGGCT ACGGCAAGCC AGTGGACTGG TGGGCTATGG GGATCATCCT CTACGAGTTC 
CTGGTGGGCT GTGTGCCCTT CTTCGGAGAC ACACCAGAGG AGCTATTTGG ACAGGTCATC 
AGTGATGACA TCCTGTGGCC CGAGGGGGAT GAGGCCCTAC CTACGGAGGC CCAACTCCTC 
ATATCCAGCC TCCTGCAGAC CAACCCTCTG GTCAGGCTTG GGGCAGGCGG CGCTTTTGAG 
GTGAAGCAGC ACAGTTTCTT TCGAGACCTG GACTGGACAG GGCTGCTGAG GCAGAAGGCC 
GAGTTCATCC CCCACCTAGA GTCGGAAGAT GACACTAGCT ACTTTGACAC CCGCTCAGAC 
AGGTATCACC ACGTGAACTC CTATGACGAG GATGACACGA CGGAGGAGGA GCCCGTGGAA 
ATCCGCCAGT TCTCTTCCTG CTCTCCGCGC TTCAGCAAGG TGTATAGCAG CATGGAGCAG 
CTGTCGCAGC ACGAGCCCAA GACCCCAGTA GCAGCTGCAG GGAGCAGCAA GCGGGAGCCG 
AGCACCAAGG GCCCCGAGGA GAAGGTGGCC GGCAAGCGGG AGGGGCTGGG CGGCCTGACC 
CTGCGTGAGA AGACCTGGAG AGGGGGCTCT CCGGAGATCA AGCGATTCTC CGCGTCCGAG 
GCCAGTTTCC TGGAGGGAGA GGCCAGTCCC CCTTTGGGCG CCCGCCGCCG TTTCTCGGCG 
CTGCTGGAGC CCAGCCGCTT CAGCGCCCCC CAAGAGGACG AGGATGAGGC CCGGCTGCGC 
AGGCCTCCCC GGCCCAGCTC CGACCCCGCG GGATCCCTGG ATGCACGGGC CCCCAAAGAG 
GAGACTCAAG GGGAAGGCAC CTCCAGCGCC GGGGACTCCG AGGCCACTGA CCGTCCACGC 
CCAGGTGACC TCTGCCCACC CTCGAAGGAT GGGGATGCAT CAGGCCCAAG GGCTACCAAT 
GACTTGGTTC TGCGCCGGGC GCGGCACCAG CAGATGTCAG GGGATGTGGC AGTAGAGAAG 
AGGCCTTCTC GAACTGGGGG CAAAGTCATC AAATCAGCCT CAGCCACTGC CTTATCTGTC 
ATGATTCCTG CAGTGGACCC ACATGGAAGT TCACCCCTTG CTAGTCCCAT GTCTCCACGA 
TCTCTGTCCT CCAACCCATC CTCACGGGAC TCCTCACCCA GCCGGGACTA CTCACCAGCT 
GTCAGTGGGC TCCGCTCCCC CATCACCATC CAGCGCTCGG GCAAGAAGTA TGGCTTCACA 
CTGCGTGCCA TCCGTGTCTA CATGGGTGAC ACGGATGTCT ATAGTGTCCA CCACATTGTC 
TGGCATGTGG AGGAAGGAGG CCCAGCCCAG GAGGCAGGAC TCTGTGCTGG GGACCTCATC 
ACCCACGTGA ATGGGGAGCC TGTGCATGGC ATGGTGCATC CTGAGGTCGT GGAGCTGATC 
CTTAAGAGTG GCAACAAGGT AGCAGTGACC ACAACGCCCT TCGAAAATAC CTCTATCCGC 
ATTGGTCCCG CAAGGCGCAG CAGCTACAAG GCTAAAATGG CTCGGAGGAA CAAGCGACCC 
TCCGCCAAGG AGGGCCAGGA GAGCAAGAAG CGCAGCTCCC TCTTCCGGAA GATCACGAAG 
CAGTCGAACC TGCTGCATAC TAGCCGCTCG CTGTCGTCGC TGAACCGCTC GCTGTCATCC 
AGCGATAGTC TCCCGGGCTC GCCTACGCAC GGGCTGCCGG CGCGCTCGCC CACGCACAGC 
TACCGCTCCA CGCCTGACTC CGCCTACCTA GGCGCCTCAT CCCAGAGCAG CTCCCCAGCC 
TCGAGCACGC CCAACTCGCC TGCGTCGTCG GCGTCGCACC ACATTCGGCC CAGCACGCTG 
CACGGACTGT CGCCAAAGCT CCATCGCCAG TACCGCTCTG CGCGATGCAA GTCGGCCGGC 
AACATCCCTC TATCGCCGCT GGCACACACG CCGTCCCCCA CGCAGGCGTC ACCGCCGCCA 
CTGCCGGGCC ACACGGTGGG CAGCTCGCAC ACTACTCAGA GCTTCCCGGC CAAACTGCAC 
TCATCGCCTC CCGTCGTGCG CCCGCGCCCC AAGAGTGCCG AGCCCCCTCG CTCGCCGCTC 
CTCAAGCGCG TGCAGTCGGC CGAGAAGCTG GGAGCCTCTT TGAGTGCGGA CAAGAAGGGC 
GCGCTGCGCA AACACAGCCT CGAGGTGGGC CACCCGGATT TCCGCAAGGA CTTCCATGGC 
GAGCTGGCGC TGCATAGCCT TGCCGAGTCC GACGGTGAGA CGCCCCCAGT CGAGGGCCTT 
GGCGCGCCCC GGCAGGTCGC CGTCCGCCGC CTGGGCCGAC AGGAGTCACC TTTGAGCCTG 
GGCGCGGACC CGTTGCTGCC CGAGGGTGCC TCCAGGCCAC CAGTGTCGAG CAAGGAGAAG 
GAATCCCCGG GGGGCGCCGA GGCGTGCACC CCACCCCGCG CGACGACCCC CGGTGGCCGG 
ACCCTGGAGC GGGACGTCGG CTGCACGCGG CATCAGAGCG TGCAGACGGA GGATGGCACT 
GGCGGGATGG CCAGGGCTGT GGCCAAGGCG GCGCTGAGCC CGGTGCAGGA ACACGAGACA 
GGCCGGCGCA GCAGCTCTGG CGAGGCGGGC ACACCCCTGG TACCCATTGT CGTAGAGCCT 
GCGCGGCCCG GGGCTAAGGC TGTGGTGCCT CAGCCTCTGG GCGCGGACTC CAAGGGGTTG 
CAGGAACCCG CACCCCTGGC GCCTTCCGTG CCCGAGGCCC CCCGGGGCCG GGAGCGCTGG 
GTGTTGGAGG TGGTGGAGGA GCGCACCACG CTGAGCGGTC CTCGCTCCAA GCCCGCCTCC 
CCAAAGCTCT CCCCGGAGCC CCAGACACCC TCCCTAGCCC CAGCGAAGTG CAGTGCACCC 
AGCAGTGCAG TGACCCCAGT CCCACCCGCA TCCCTCTTGG GCTCAGGCAC CAAGCCTCAA 
GTGGGGCTGA CCTCCCGGTG CCCTGCTGAA GCTGTGCCCC CAGCAGGCCT GACCAAAAAA 
GGAGTGTCCA GTCCCGCACC CCCGGGACCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055790.1
CDS	59..4771
Translation	MSDSLWTALSNFSMPSFPGGSMFRRTKSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFTRAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVHLEEQDSGGSNTPEQDDLSEGRSSKAKKPPGENDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVAAAGSSKREPSTKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFSAPQEDEDEARLRRPPRPSSDPAGSLDARAPKEETQGEGTSSAGDSEATDRPRPGDLCPPSKDGDASGPRATNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLSADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPVEGLGAPRQVAVRRLGRQESPLSLGADPLLPEGASRPPVSSKEKESPGGAEACTPPRATTPGGRTLERDVGCTRHQSVQTEDGTGGMARAVAKAALSPVQEHETGRRSSSGEAGTPLVPIVVEPARPGAKAVVPQPLGADSKGLQEPAPLAPSVPEAPRGRERWVLEVVEERTTLSGPRSKPASPKLSPEPQTPSLAPAKCSAPSSAVTPVPPASLLGSGTKPQVGLTSRCPAEAVPPAGLTKKGVSSPAPPGP

Target ORF information:

RefSeq Version	NM_014975.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014975.3

ORF Insert Sequence:

ATGTCTGACT CTCTCTGGAC CGCGCTTTCT AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGTATGTTCC GCCGCACCAA GAGCTGCCGC ACCAGTAATC GGAAAAGCCT CATCCTGACC 
AGCACTTCAC CCACGCTACC GAGACCCCAC TCCCCGCTGC CAGGTCACCT AGGCAGCAGT 
CCCCTGGACA GCCCCCGAAA CTTCTCCCCC AACACCCCCG CCCACTTCTC GTTTGCCTCC 
TCCCGAAGGG CGGACGGACG CCGGTGGTCT CTGGCCTCGC TCCCTTCATC TGGCTATGGC 
ACCAACACGC CCAGTTCCAC CGTCTCGTCC TCCTGCTCCT CCCAGGAGCG CCTTCACCAG 
CTGCCCTACC AGCCCACGGT GGACGAGCTC CACTTCCTCT CCAAACACTT CGGGAGCACC 
GAGAGCATCA CAGACGAGGA TGGTGGCCGT CGCTCCCCAG CCGTGCGGCC CCGCTCACGG 
AGCCTCAGCC CCGGGCGCTC CCCCTCCTCC TACGACAACG AGATCGTGAT GATGAATCAC 
GTCTACAAGG AGAGGTTCCC GAAGGCCACT GCGCAGATGG AGGAGAAGCT GCGCGACTTT 
ACCCGCGCCT ACGAACCCGA CAGCGTTCTG CCTCTGGCCG ATGGCGTGCT CAGCTTCATC 
CACCACCAGA TCATCGAGCT GGCCCGGGAC TGCCTGACCA AGTCCCGTGA CGGCCTCATC 
ACCACGGTCT ACTTCTATGA ATTGCAGGAG AACCTGGAGA AGCTCCTTCA AGACGCCTAT 
GAACGCTCTG AGAGCTTGGA GGTGGCCTTC GTTACTCAGC TGGTGAAGAA GTTGCTTATT 
ATCATCTCAC GCCCTGCGAG GCTGCTGGAG TGCCTGGAAT TCAACCCCGA GGAGTTCTAC 
CACCTGCTGG AGGCGGCCGA AGGACACGCC AAGGAGGGCC ACCTTGTGAA GACGGACATC 
CCCCGCTACA TCATCCGCCA GCTGGGCCTC ACCCGTGACC CCTTTCCAGA TGTGGTGCAT 
CTGGAGGAAC AGGACAGTGG TGGTTCCAAC ACCCCTGAGC AAGACGATCT CTCTGAGGGC 
CGCAGCAGCA AGGCCAAGAA ACCGCCGGGG GAGAATGACT TCGATACCAT CAAGCTCATA 
AGCAACGGTG CCTACGGCGC TGTCTACCTG GTGCGGCACC GCGACACGCG GCAGCGCTTT 
GCCATGAAAA AGATCAACAA GCAGAACTTG ATCCTCCGCA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGCG ATATCCTCAC CTTCGCCGAG AACCCGTTTG TGGTCGGCAT GTTCTGCTCC 
TTTGAGACTC GGCGCCACCT CTGCATGGTC ATGGAATATG TGGAAGGCGG CGACTGTGCC 
ACCCTGCTGA AGAATATTGG AGCGCTGCCC GTAGAGATGG CCCGCATGTA CTTTGCTGAG 
ACGGTGCTAG CCCTGGAGTA TTTGCACAAC TATGGCATCG TGCACCGCGA CCTCAAGCCT 
GACAACCTCC TTATCACCTC CATGGGTCAC ATCAAGCTCA CAGATTTCGG CCTCTCCAAG 
ATGGGGCTCA TGAGCCTCAC CACCAACTTA TATGAAGGCC ACATCGAGAA GGACGCCCGA 
GAGTTCCTGG ACAAACAGGT GTGTGGGACC CCAGAGTACA TCGCGCCCGA GGTCATCCTG 
CGTCAAGGCT ACGGCAAGCC AGTGGACTGG TGGGCTATGG GGATCATCCT CTACGAGTTC 
CTGGTGGGCT GTGTGCCCTT CTTCGGAGAC ACACCAGAGG AGCTATTTGG ACAGGTCATC 
AGTGATGACA TCCTGTGGCC CGAGGGGGAT GAGGCCCTAC CTACGGAGGC CCAACTCCTC 
ATATCCAGCC TCCTGCAGAC CAACCCTCTG GTCAGGCTTG GGGCAGGCGG CGCTTTTGAG 
GTGAAGCAGC ACAGTTTCTT TCGAGACCTG GACTGGACAG GGCTGCTGAG GCAGAAGGCC 
GAGTTCATCC CCCACCTAGA GTCGGAAGAT GACACTAGCT ACTTTGACAC CCGCTCAGAC 
AGGTATCACC ACGTGAACTC CTATGACGAG GATGACACGA CGGAGGAGGA GCCCGTGGAA 
ATCCGCCAGT TCTCTTCCTG CTCTCCGCGC TTCAGCAAGG TGTATAGCAG CATGGAGCAG 
CTGTCGCAGC ACGAGCCCAA GACCCCAGTA GCAGCTGCAG GGAGCAGCAA GCGGGAGCCG 
AGCACCAAGG GCCCCGAGGA GAAGGTGGCC GGCAAGCGGG AGGGGCTGGG CGGCCTGACC 
CTGCGTGAGA AGACCTGGAG AGGGGGCTCT CCGGAGATCA AGCGATTCTC CGCGTCCGAG 
GCCAGTTTCC TGGAGGGAGA GGCCAGTCCC CCTTTGGGCG CCCGCCGCCG TTTCTCGGCG 
CTGCTGGAGC CCAGCCGCTT CAGCGCCCCC CAAGAGGACG AGGATGAGGC CCGGCTGCGC 
AGGCCTCCCC GGCCCAGCTC CGACCCCGCG GGATCCCTGG ATGCACGGGC CCCCAAAGAG 
GAGACTCAAG GGGAAGGCAC CTCCAGCGCC GGGGACTCCG AGGCCACTGA CCGTCCACGC 
CCAGGTGACC TCTGCCCACC CTCGAAGGAT GGGGATGCAT CAGGCCCAAG GGCTACCAAT 
GACTTGGTTC TGCGCCGGGC GCGGCACCAG CAGATGTCAG GGGATGTGGC AGTAGAGAAG 
AGGCCTTCTC GAACTGGGGG CAAAGTCATC AAATCAGCCT CAGCCACTGC CTTATCTGTC 
ATGATTCCTG CAGTGGACCC ACATGGAAGT TCACCCCTTG CTAGTCCCAT GTCTCCACGA 
TCTCTGTCCT CCAACCCATC CTCACGGGAC TCCTCACCCA GCCGGGACTA CTCACCAGCT 
GTCAGTGGGC TCCGCTCCCC CATCACCATC CAGCGCTCGG GCAAGAAGTA TGGCTTCACA 
CTGCGTGCCA TCCGTGTCTA CATGGGTGAC ACGGATGTCT ATAGTGTCCA CCACATTGTC 
TGGCATGTGG AGGAAGGAGG CCCAGCCCAG GAGGCAGGAC TCTGTGCTGG GGACCTCATC 
ACCCACGTGA ATGGGGAGCC TGTGCATGGC ATGGTGCATC CTGAGGTCGT GGAGCTGATC 
CTTAAGAGTG GCAACAAGGT AGCAGTGACC ACAACGCCCT TCGAAAATAC CTCTATCCGC 
ATTGGTCCCG CAAGGCGCAG CAGCTACAAG GCTAAAATGG CTCGGAGGAA CAAGCGACCC 
TCCGCCAAGG AGGGCCAGGA GAGCAAGAAG CGCAGCTCCC TCTTCCGGAA GATCACGAAG 
CAGTCGAACC TGCTGCATAC TAGCCGCTCG CTGTCGTCGC TGAACCGCTC GCTGTCATCC 
AGCGATAGTC TCCCGGGCTC GCCTACGCAC GGGCTGCCGG CGCGCTCGCC CACGCACAGC 
TACCGCTCCA CGCCTGACTC CGCCTACCTA GGCGCCTCAT CCCAGAGCAG CTCCCCAGCC 
TCGAGCACGC CCAACTCGCC TGCGTCGTCG GCGTCGCACC ACATTCGGCC CAGCACGCTG 
CACGGACTGT CGCCAAAGCT CCATCGCCAG TACCGCTCTG CGCGATGCAA GTCGGCCGGC 
AACATCCCTC TATCGCCGCT GGCACACACG CCGTCCCCCA CGCAGGCGTC ACCGCCGCCA 
CTGCCGGGCC ACACGGTGGG CAGCTCGCAC ACTACTCAGA GCTTCCCGGC CAAACTGCAC 
TCATCGCCTC CCGTCGTGCG CCCGCGCCCC AAGAGTGCCG AGCCCCCTCG CTCGCCGCTC 
CTCAAGCGCG TGCAGTCGGC CGAGAAGCTG GGAGCCTCTT TGAGTGCGGA CAAGAAGGGC 
GCGCTGCGCA AACACAGCCT CGAGGTGGGC CACCCGGATT TCCGCAAGGA CTTCCATGGC 
GAGCTGGCGC TGCATAGCCT TGCCGAGTCC GACGGTGAGA CGCCCCCAGT CGAGGGCCTT 
GGCGCGCCCC GGCAGGTCGC CGTCCGCCGC CTGGGCCGAC AGGAGTCACC TTTGAGCCTG 
GGCGCGGACC CGTTGCTGCC CGAGGGTGCC TCCAGGCCAC CAGTGTCGAG CAAGGAGAAG 
GAATCCCCGG GGGGCGCCGA GGCGTGCACC CCACCCCGCG CGACGACCCC CGGTGGCCGG 
ACCCTGGAGC GGGACGTCGG CTGCACGCGG CATCAGAGCG TGCAGACGGA GGATGGCACT 
GGCGGGATGG CCAGGGCTGT GGCCAAGGCG GCGCTGAGCC CGGTGCAGGA ACACGAGACA 
GGCCGGCGCA GCAGCTCTGG CGAGGCGGGC ACACCCCTGG TACCCATTGT CGTAGAGCCT 
GCGCGGCCCG GGGCTAAGGC TGTGGTGCCT CAGCCTCTGG GCGCGGACTC CAAGGGGTTG 
CAGGAACCCG CACCCCTGGC GCCTTCCGTG CCCGAGGCCC CCCGGGGCCG GGAGCGCTGG 
GTGTTGGAGG TGGTGGAGGA GCGCACCACG CTGAGCGGTC CTCGCTCCAA GCCCGCCTCC 
CCAAAGCTCT CCCCGGAGCC CCAGACACCC TCCCTAGCCC CAGCGAAGTG CAGTGCACCC 
AGCAGTGCAG TGACCCCAGT CCCACCCGCA TCCCTCTTGG GCTCAGGCAC CAAGCCTCAA 
GTGGGGCTGA CCTCCCGGTG CCCTGCTGAA GCTGTGCCCC CAGCAGGCCT GACCAAAAAA 
GGAGTGTCCA GTCCCGCACC CCCGGGACCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST1 ( XM_011527805.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526107.1 Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu65209
Clone ID Related Accession (Same CDS sequence)	XM_011527805.2
Accession Version	XM_011527805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATT CGGGCCTCAT CCGCCGCCGG AGGCTGCAGA AGGATTTGCC CCTGCCCCGG 
AAAAGCAGCA GCTGCCGCAC CAGTAATCGG AAAAGCCTCA TCCTGACCAG CACTTCACCC 
ACGCTACCGA GACCCCACTC CCCGCTGCCA GGTCACCTAG GCAGCAGTCC CCTGGACAGC 
CCCCGAAACT TCTCCCCCAA CACCCCCGCC CACTTCTCGT TTGCCTCCTC CCGAAGGGCG 
GACGGACGCC GGTGGTCTCT GGCCTCGCTC CCTTCATCTG GCTATGGCAC CAACACGCCC 
AGTTCCACCG TCTCGTCCTC CTGCTCCTCC CAGGAGCGCC TTCACCAGCT GCCCTACCAG 
CCCACGGTGG ACGAGCTCCA CTTCCTCTCC AAACACTTCG GGAGCACCGA GAGCATCACA 
GACGAGGATG GTGGCCGTCG CTCCCCAGCC GTGCGGCCCC GCTCACGGAG CCTCAGCCCC 
GGGCGCTCCC CCTCCTCCTA CGACAACGAG ATCGTGATGA TGAATCACGT CTACAAGGAG 
AGGTTCCCGA AGGCCACTGC GCAGATGGAG GAGAAGCTGC GCGACTTTAC CCGCGCCTAC 
GAACCCGACA GCGTTCTGCC TCTGGCCGAT GGCGTGCTCA GCTTCATCCA CCACCAGATC 
ATCGAGCTGG CCCGGGACTG CCTGACCAAG TCCCGTGACG GCCTCATCAC CACGGTCTAC 
TTCTATGAAT TGCAGGAGAA CCTGGAGAAG CTCCTTCAAG ACGCCTATGA ACGCTCTGAG 
AGCTTGGAGG TGGCCTTCGT TACTCAGCTG GTGAAGAAGT TGCTTATTAT CATCTCACGC 
CCTGCGAGGC TGCTGGAGTG CCTGGAATTC AACCCCGAGG AGTTCTACCA CCTGCTGGAG 
GCGGCCGAAG GACACGCCAA GGAGGGCCAC CTTGTGAAGA CGGACATCCC CCGCTACATC 
ATCCGCCAGC TGGGCCTCAC CCGTGACCCC TTTCCAGATG TGGTGCATCT GGAGGAACAG 
GACAGTGGTG GTTCCAACAC CCCTGAGCAA GACGATCTCT CTGAGGGCCG CAGCAGCAAG 
GCCAAGAAAC CGCCGGGGGA GAATGACTTC GATACCATCA AGCTCATAAG CAACGGTGCC 
TACGGCGCTG TCTACCTGGT GCGGCACCGC GACACGCGGC AGCGCTTTGC CATGAAAAAG 
ATCAACAAGC AGAACTTGAT CCTCCGCAAC CAGATCCAGC AGGCCTTTGT GGAGCGCGAT 
ATCCTCACCT TCGCCGAGAA CCCGTTTGTG GTCGGCATGT TCTGCTCCTT TGAGACTCGG 
CGCCACCTCT GCATGGTCAT GGAATATGTG GAAGGCGGCG ACTGTGCCAC CCTGCTGAAG 
AATATTGGAG CGCTGCCCGT AGAGATGGCC CGCATGTACT TTGCTGAGAC GGTGCTAGCC 
CTGGAGTATT TGCACAACTA TGGCATCGTG CACCGCGACC TCAAGCCTGA CAACCTCCTT 
ATCACCTCCA TGGGTCACAT CAAGCTCACA GATTTCGGCC TCTCCAAGAT GGGGCTCATG 
AGCCTCACCA CCAACTTATA TGAAGGCCAC ATCGAGAAGG ACGCCCGAGA GTTCCTGGAC 
AAACAGGTGT GTGGGACCCC AGAGTACATC GCGCCCGAGG TCATCCTGCG TCAAGGCTAC 
GGCAAGCCAG TGGACTGGTG GGCTATGGGG ATCATCCTCT ACGAGTTCCT GGTGGGCTGT 
GTGCCCTTCT TCGGAGACAC ACCAGAGGAG CTATTTGGAC AGGTCATCAG TGATGACATC 
CTGTGGCCCG AGGGGGATGA GGCCCTACCT ACGGAGGCCC AACTCCTCAT ATCCAGCCTC 
CTGCAGACCA ACCCTCTGGT CAGGCTTGGG GCAGGCGGCG CTTTTGAGGT GAAGCAGCAC 
AGTTTCTTTC GAGACCTGGA CTGGACAGGG CTGCTGAGGC AGAAGGCCGA GTTCATCCCC 
CACCTAGAGT CGGAAGATGA CACTAGCTAC TTTGACACCC GCTCAGACAG GTATCACCAC 
GTGAACTCCT ATGACGAGGA TGACACGACG GAGGAGGAGC CCGTGGAAAT CCGCCAGTTC 
TCTTCCTGCT CTCCGCGCTT CAGCAAGGTG TATAGCAGCA TGGAGCAGCT GTCGCAGCAC 
GAGCCCAAGA CCCCAGTAGC AGCTGCAGGG AGCAGCAAGC GGGAGCCGAG CACCAAGGGC 
CCCGAGGAGA AGGTGGCCGG CAAGCGGGAG GGGCTGGGCG GCCTGACCCT GCGTGAGAAG 
ACCTGGAGAG GGGGCTCTCC GGAGATCAAG CGATTCTCCG CGTCCGAGGC CAGTTTCCTG 
GAGGGAGAGG CCAGTCCCCC TTTGGGCGCC CGCCGCCGTT TCTCGGCGCT GCTGGAGCCC 
AGCCGCTTCA GCGCCCCCCA AGAGGACGAG GATGAGGCCC GGCTGCGCAG GCCTCCCCGG 
CCCAGCTCCG ACCCCGCGGG ATCCCTGGAT GCACGGGCCC CCAAAGAGGA GACTCAAGGG 
GAAGGCACCT CCAGCGCCGG GGACTCCGAG GCCACTGACC GTCCACGCCC AGGTGACCTC 
TGCCCACCCT CGAAGGATGG GGATGCATCA GGCCCAAGGG CTACCAATGA CTTGGTTCTG 
CGCCGGGCGC GGCACCAGCA GATGTCAGGG GATGTGGCAG TAGAGAAGAG GCCTTCTCGA 
ACTGGGGGCA AAGTCATCAA ATCAGCCTCA GCCACTGCCT TATCTGTCAT GATTCCTGCA 
GTGGACCCAC ATGGAAGTTC ACCCCTTGCT AGTCCCATGT CTCCACGATC TCTGTCCTCC 
AACCCATCCT CACGGGACTC CTCACCCAGC CGGGACTACT CACCAGCTGT CAGTGGGCTC 
CGCTCCCCCA TCACCATCCA GCGCTCGGGC AAGAAGTATG GCTTCACACT GCGTGCCATC 
CGTGTCTACA TGGGTGACAC GGATGTCTAT AGTGTCCACC ACATTGTCTG GCATGTGGAG 
GAAGGAGGCC CAGCCCAGGA GGCAGGACTC TGTGCTGGGG ACCTCATCAC CCACGTGAAT 
GGGGAGCCTG TGCATGGCAT GGTGCATCCT GAGGTCGTGG AGCTGATCCT TAAGAGTGGC 
AACAAGGTAG CAGTGACCAC AACGCCCTTC GAAAATACCT CTATCCGCAT TGGTCCCGCA 
AGGCGCAGCA GCTACAAGGC TAAAATGGCT CGGAGGAACA AGCGACCCTC CGCCAAGGAG 
GGCCAGGAGA GCAAGAAGCG CAGCTCCCTC TTCCGGAAGA TCACGAAGCA GTCGAACCTG 
CTGCATACTA GCCGCTCGCT GTCGTCGCTG AACCGCTCGC TGTCATCCAG CGATAGTCTC 
CCGGGCTCGC CTACGCACGG GCTGCCGGCG CGCTCGCCCA CGCACAGCTA CCGCTCCACG 
CCTGACTCCG CCTACCTAGG CGCCTCATCC CAGAGCAGCT CCCCAGCCTC GAGCACGCCC 
AACTCGCCTG CGTCGTCGGC GTCGCACCAC ATTCGGCCCA GCACGCTGCA CGGACTGTCG 
CCAAAGCTCC ATCGCCAGTA CCGCTCTGCG CGATGCAAGT CGGCCGGCAA CATCCCTCTA 
TCGCCGCTGG CACACACGCC GTCCCCCACG CAGGCGTCAC CGCCGCCACT GCCGGGCCAC 
ACGGTGGGCA GCTCGCACAC TACTCAGAGC TTCCCGGCCA AACTGCACTC ATCGCCTCCC 
GTCGTGCGCC CGCGCCCCAA GAGTGCCGAG CCCCCTCGCT CGCCGCTCCT CAAGCGCGTG 
CAGTCGGCCG AGAAGCTGGG AGCCTCTTTG AGTGCGGACA AGAAGGGCGC GCTGCGCAAA 
CACAGCCTCG AGGTGGGCCA CCCGGATTTC CGCAAGGACT TCCATGGCGA GCTGGCGCTG 
CATAGCCTTG CCGAGTCCGA CGGTGAGACG CCCCCAGTCG AGGGCCTTGG CGCGCCCCGG 
CAGGTCGCCG TCCGCCGCCT GGGCCGACAG GAGTCACCTT TGAGCCTGGG CGCGGACCCG 
TTGCTGCCCG AGGGTGCCTC CAGGCCACCA GTGTCGAGCA AGGAGAAGGA ATCCCCGGGG 
GGCGCCGAGG CGTGCACCCC ACCCCGCGCG ACGACCCCCG GTGGCCGGAC CCTGGAGCGG 
GACGTCGGCT GCACGCGGCA TCAGAGCGTG CAGACGGAGG ATGGCACTGG CGGGATGGCC 
AGGGCTGTGG CCAAGGCGGC GCTGAGCCCG GTGCAGGAAC ACGAGACAGG CCGGCGCAGC 
AGCTCTGGCG AGGCGGGCAC ACCCCTGGTA CCCATTGTCG TAGAGCCTGC GCGGCCCGGG 
GCTAAGGCTG TGGTGCCTCA GCCTCTGGGC GCGGACTCCA AGGGGTTGCA GGAACCCGCA 
CCCCTGGCGC CTTCCGTGCC CGAGGCCCCC CGGGGCCGGG AGCGCTGGGT GTTGGAGGTG 
GTGGAGGAGC GCACCACGCT GAGCGGTCCT CGCTCCAAGC CCGCCTCCCC AAAGCTCTCC 
CCGGAGCCCC AGACACCCTC CCTAGCCCCA GCGAAGTGCA GTGCACCCAG CAGTGCAGTG 
ACCCCAGTCC CACCCGCATC CCTCTTGGGC TCAGGCACCA AGCCTCAAGT GGGGCTGACC 
TCCCGGTGCC CTGCTGAAGC TGTGCCCCCA GCAGGCCTGA CCAAAAAAGG AGTGTCCAGT 
CCCGCACCCC CGGGACCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526107.1
CDS	38..4738
Translation	MDDSGLIRRRRLQKDLPLPRKSSSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFTRAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVHLEEQDSGGSNTPEQDDLSEGRSSKAKKPPGENDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVAAAGSSKREPSTKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFSAPQEDEDEARLRRPPRPSSDPAGSLDARAPKEETQGEGTSSAGDSEATDRPRPGDLCPPSKDGDASGPRATNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLSADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPVEGLGAPRQVAVRRLGRQESPLSLGADPLLPEGASRPPVSSKEKESPGGAEACTPPRATTPGGRTLERDVGCTRHQSVQTEDGTGGMARAVAKAALSPVQEHETGRRSSSGEAGTPLVPIVVEPARPGAKAVVPQPLGADSKGLQEPAPLAPSVPEAPRGRERWVLEVVEERTTLSGPRSKPASPKLSPEPQTPSLAPAKCSAPSSAVTPVPPASLLGSGTKPQVGLTSRCPAEAVPPAGLTKKGVSSPAPPGP

Target ORF information:

RefSeq Version	XM_011527805.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527805.2

ORF Insert Sequence:

ATGGACGATT CGGGCCTCAT CCGCCGCCGG AGGCTGCAGA AGGATTTGCC CCTGCCCCGG 
AAAAGCAGCA GCTGCCGCAC CAGTAATCGG AAAAGCCTCA TCCTGACCAG CACTTCACCC 
ACGCTACCGA GACCCCACTC CCCGCTGCCA GGTCACCTAG GCAGCAGTCC CCTGGACAGC 
CCCCGAAACT TCTCCCCCAA CACCCCCGCC CACTTCTCGT TTGCCTCCTC CCGAAGGGCG 
GACGGACGCC GGTGGTCTCT GGCCTCGCTC CCTTCATCTG GCTATGGCAC CAACACGCCC 
AGTTCCACCG TCTCGTCCTC CTGCTCCTCC CAGGAGCGCC TTCACCAGCT GCCCTACCAG 
CCCACGGTGG ACGAGCTCCA CTTCCTCTCC AAACACTTCG GGAGCACCGA GAGCATCACA 
GACGAGGATG GTGGCCGTCG CTCCCCAGCC GTGCGGCCCC GCTCACGGAG CCTCAGCCCC 
GGGCGCTCCC CCTCCTCCTA CGACAACGAG ATCGTGATGA TGAATCACGT CTACAAGGAG 
AGGTTCCCGA AGGCCACTGC GCAGATGGAG GAGAAGCTGC GCGACTTTAC CCGCGCCTAC 
GAACCCGACA GCGTTCTGCC TCTGGCCGAT GGCGTGCTCA GCTTCATCCA CCACCAGATC 
ATCGAGCTGG CCCGGGACTG CCTGACCAAG TCCCGTGACG GCCTCATCAC CACGGTCTAC 
TTCTATGAAT TGCAGGAGAA CCTGGAGAAG CTCCTTCAAG ACGCCTATGA ACGCTCTGAG 
AGCTTGGAGG TGGCCTTCGT TACTCAGCTG GTGAAGAAGT TGCTTATTAT CATCTCACGC 
CCTGCGAGGC TGCTGGAGTG CCTGGAATTC AACCCCGAGG AGTTCTACCA CCTGCTGGAG 
GCGGCCGAAG GACACGCCAA GGAGGGCCAC CTTGTGAAGA CGGACATCCC CCGCTACATC 
ATCCGCCAGC TGGGCCTCAC CCGTGACCCC TTTCCAGATG TGGTGCATCT GGAGGAACAG 
GACAGTGGTG GTTCCAACAC CCCTGAGCAA GACGATCTCT CTGAGGGCCG CAGCAGCAAG 
GCCAAGAAAC CGCCGGGGGA GAATGACTTC GATACCATCA AGCTCATAAG CAACGGTGCC 
TACGGCGCTG TCTACCTGGT GCGGCACCGC GACACGCGGC AGCGCTTTGC CATGAAAAAG 
ATCAACAAGC AGAACTTGAT CCTCCGCAAC CAGATCCAGC AGGCCTTTGT GGAGCGCGAT 
ATCCTCACCT TCGCCGAGAA CCCGTTTGTG GTCGGCATGT TCTGCTCCTT TGAGACTCGG 
CGCCACCTCT GCATGGTCAT GGAATATGTG GAAGGCGGCG ACTGTGCCAC CCTGCTGAAG 
AATATTGGAG CGCTGCCCGT AGAGATGGCC CGCATGTACT TTGCTGAGAC GGTGCTAGCC 
CTGGAGTATT TGCACAACTA TGGCATCGTG CACCGCGACC TCAAGCCTGA CAACCTCCTT 
ATCACCTCCA TGGGTCACAT CAAGCTCACA GATTTCGGCC TCTCCAAGAT GGGGCTCATG 
AGCCTCACCA CCAACTTATA TGAAGGCCAC ATCGAGAAGG ACGCCCGAGA GTTCCTGGAC 
AAACAGGTGT GTGGGACCCC AGAGTACATC GCGCCCGAGG TCATCCTGCG TCAAGGCTAC 
GGCAAGCCAG TGGACTGGTG GGCTATGGGG ATCATCCTCT ACGAGTTCCT GGTGGGCTGT 
GTGCCCTTCT TCGGAGACAC ACCAGAGGAG CTATTTGGAC AGGTCATCAG TGATGACATC 
CTGTGGCCCG AGGGGGATGA GGCCCTACCT ACGGAGGCCC AACTCCTCAT ATCCAGCCTC 
CTGCAGACCA ACCCTCTGGT CAGGCTTGGG GCAGGCGGCG CTTTTGAGGT GAAGCAGCAC 
AGTTTCTTTC GAGACCTGGA CTGGACAGGG CTGCTGAGGC AGAAGGCCGA GTTCATCCCC 
CACCTAGAGT CGGAAGATGA CACTAGCTAC TTTGACACCC GCTCAGACAG GTATCACCAC 
GTGAACTCCT ATGACGAGGA TGACACGACG GAGGAGGAGC CCGTGGAAAT CCGCCAGTTC 
TCTTCCTGCT CTCCGCGCTT CAGCAAGGTG TATAGCAGCA TGGAGCAGCT GTCGCAGCAC 
GAGCCCAAGA CCCCAGTAGC AGCTGCAGGG AGCAGCAAGC GGGAGCCGAG CACCAAGGGC 
CCCGAGGAGA AGGTGGCCGG CAAGCGGGAG GGGCTGGGCG GCCTGACCCT GCGTGAGAAG 
ACCTGGAGAG GGGGCTCTCC GGAGATCAAG CGATTCTCCG CGTCCGAGGC CAGTTTCCTG 
GAGGGAGAGG CCAGTCCCCC TTTGGGCGCC CGCCGCCGTT TCTCGGCGCT GCTGGAGCCC 
AGCCGCTTCA GCGCCCCCCA AGAGGACGAG GATGAGGCCC GGCTGCGCAG GCCTCCCCGG 
CCCAGCTCCG ACCCCGCGGG ATCCCTGGAT GCACGGGCCC CCAAAGAGGA GACTCAAGGG 
GAAGGCACCT CCAGCGCCGG GGACTCCGAG GCCACTGACC GTCCACGCCC AGGTGACCTC 
TGCCCACCCT CGAAGGATGG GGATGCATCA GGCCCAAGGG CTACCAATGA CTTGGTTCTG 
CGCCGGGCGC GGCACCAGCA GATGTCAGGG GATGTGGCAG TAGAGAAGAG GCCTTCTCGA 
ACTGGGGGCA AAGTCATCAA ATCAGCCTCA GCCACTGCCT TATCTGTCAT GATTCCTGCA 
GTGGACCCAC ATGGAAGTTC ACCCCTTGCT AGTCCCATGT CTCCACGATC TCTGTCCTCC 
AACCCATCCT CACGGGACTC CTCACCCAGC CGGGACTACT CACCAGCTGT CAGTGGGCTC 
CGCTCCCCCA TCACCATCCA GCGCTCGGGC AAGAAGTATG GCTTCACACT GCGTGCCATC 
CGTGTCTACA TGGGTGACAC GGATGTCTAT AGTGTCCACC ACATTGTCTG GCATGTGGAG 
GAAGGAGGCC CAGCCCAGGA GGCAGGACTC TGTGCTGGGG ACCTCATCAC CCACGTGAAT 
GGGGAGCCTG TGCATGGCAT GGTGCATCCT GAGGTCGTGG AGCTGATCCT TAAGAGTGGC 
AACAAGGTAG CAGTGACCAC AACGCCCTTC GAAAATACCT CTATCCGCAT TGGTCCCGCA 
AGGCGCAGCA GCTACAAGGC TAAAATGGCT CGGAGGAACA AGCGACCCTC CGCCAAGGAG 
GGCCAGGAGA GCAAGAAGCG CAGCTCCCTC TTCCGGAAGA TCACGAAGCA GTCGAACCTG 
CTGCATACTA GCCGCTCGCT GTCGTCGCTG AACCGCTCGC TGTCATCCAG CGATAGTCTC 
CCGGGCTCGC CTACGCACGG GCTGCCGGCG CGCTCGCCCA CGCACAGCTA CCGCTCCACG 
CCTGACTCCG CCTACCTAGG CGCCTCATCC CAGAGCAGCT CCCCAGCCTC GAGCACGCCC 
AACTCGCCTG CGTCGTCGGC GTCGCACCAC ATTCGGCCCA GCACGCTGCA CGGACTGTCG 
CCAAAGCTCC ATCGCCAGTA CCGCTCTGCG CGATGCAAGT CGGCCGGCAA CATCCCTCTA 
TCGCCGCTGG CACACACGCC GTCCCCCACG CAGGCGTCAC CGCCGCCACT GCCGGGCCAC 
ACGGTGGGCA GCTCGCACAC TACTCAGAGC TTCCCGGCCA AACTGCACTC ATCGCCTCCC 
GTCGTGCGCC CGCGCCCCAA GAGTGCCGAG CCCCCTCGCT CGCCGCTCCT CAAGCGCGTG 
CAGTCGGCCG AGAAGCTGGG AGCCTCTTTG AGTGCGGACA AGAAGGGCGC GCTGCGCAAA 
CACAGCCTCG AGGTGGGCCA CCCGGATTTC CGCAAGGACT TCCATGGCGA GCTGGCGCTG 
CATAGCCTTG CCGAGTCCGA CGGTGAGACG CCCCCAGTCG AGGGCCTTGG CGCGCCCCGG 
CAGGTCGCCG TCCGCCGCCT GGGCCGACAG GAGTCACCTT TGAGCCTGGG CGCGGACCCG 
TTGCTGCCCG AGGGTGCCTC CAGGCCACCA GTGTCGAGCA AGGAGAAGGA ATCCCCGGGG 
GGCGCCGAGG CGTGCACCCC ACCCCGCGCG ACGACCCCCG GTGGCCGGAC CCTGGAGCGG 
GACGTCGGCT GCACGCGGCA TCAGAGCGTG CAGACGGAGG ATGGCACTGG CGGGATGGCC 
AGGGCTGTGG CCAAGGCGGC GCTGAGCCCG GTGCAGGAAC ACGAGACAGG CCGGCGCAGC 
AGCTCTGGCG AGGCGGGCAC ACCCCTGGTA CCCATTGTCG TAGAGCCTGC GCGGCCCGGG 
GCTAAGGCTG TGGTGCCTCA GCCTCTGGGC GCGGACTCCA AGGGGTTGCA GGAACCCGCA 
CCCCTGGCGC CTTCCGTGCC CGAGGCCCCC CGGGGCCGGG AGCGCTGGGT GTTGGAGGTG 
GTGGAGGAGC GCACCACGCT GAGCGGTCCT CGCTCCAAGC CCGCCTCCCC AAAGCTCTCC 
CCGGAGCCCC AGACACCCTC CCTAGCCCCA GCGAAGTGCA GTGCACCCAG CAGTGCAGTG 
ACCCCAGTCC CACCCGCATC CCTCTTGGGC TCAGGCACCA AGCCTCAAGT GGGGCTGACC 
TCCCGGTGCC CTGCTGAAGC TGTGCCCCCA GCAGGCCTGA CCAAAAAAGG AGTGTCCAGT 
CCCGCACCCC CGGGACCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST1 ( XM_011527808.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526110.1 Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.

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CloneID	OHu65211
Clone ID Related Accession (Same CDS sequence)	XM_011527808.2
Accession Version	XM_011527808.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTGG ATTTCATTCT GAATGCAACA GAAGGCGGCG ACTGTGCCAC CCTGCTGAAG 
AATATTGGAG CGCTGCCCGT AGAGATGGCC CGCATGTACT TTGCTGAGAC GGTGCTAGCC 
CTGGAGTATT TGCACAACTA TGGCATCGTG CACCGCGACC TCAAGCCTGA CAACCTCCTT 
ATCACCTCCA TGGGTCACAT CAAGCTCACA GATTTCGGCC TCTCCAAGAT GGGGCTCATG 
AGCCTCACCA CCAACTTATA TGAAGGCCAC ATCGAGAAGG ACGCCCGAGA GTTCCTGGAC 
AAACAGGTGT GTGGGACCCC AGAGTACATC GCGCCCGAGG TCATCCTGCG TCAAGGCTAC 
GGCAAGCCAG TGGACTGGTG GGCTATGGGG ATCATCCTCT ACGAGTTCCT GGTGGGCTGT 
GTGCCCTTCT TCGGAGACAC ACCAGAGGAG CTATTTGGAC AGGTCATCAG TGATGACATC 
CTGTGGCCCG AGGGGGATGA GGCCCTACCT ACGGAGGCCC AACTCCTCAT ATCCAGCCTC 
CTGCAGACCA ACCCTCTGGT CAGGCTTGGG GCAGGCGGCG CTTTTGAGGT GAAGCAGCAC 
AGTTTCTTTC GAGACCTGGA CTGGACAGGG CTGCTGAGGC AGAAGGCCGA GTTCATCCCC 
CACCTAGAGT CGGAAGATGA CACTAGCTAC TTTGACACCC GCTCAGACAG GTATCACCAC 
GTGAACTCCT ATGACGAGGA TGACACGACG GAGGAGGAGC CCGTGGAAAT CCGCCAGTTC 
TCTTCCTGCT CTCCGCGCTT CAGCAAGGTG TATAGCAGCA TGGAGCAGCT GTCGCAGCAC 
GAGCCCAAGA CCCCAGTAGC AGCTGCAGGG AGCAGCAAGC GGGAGCCGAG CACCAAGGGC 
CCCGAGGAGA AGGTGGCCGG CAAGCGGGAG GGGCTGGGCG GCCTGACCCT GCGTGAGAAG 
ACCTGGAGAG GGGGCTCTCC GGAGATCAAG CGATTCTCCG CGTCCGAGGC CAGTTTCCTG 
GAGGGAGAGG CCAGTCCCCC TTTGGGCGCC CGCCGCCGTT TCTCGGCGCT GCTGGAGCCC 
AGCCGCTTCA GCGCCCCCCA AGAGGACGAG GATGAGGCCC GGCTGCGCAG GCCTCCCCGG 
CCCAGCTCCG ACCCCGCGGG ATCCCTGGAT GCACGGGCCC CCAAAGAGGA GACTCAAGGG 
GAAGGCACCT CCAGCGCCGG GGACTCCGAG GCCACTGACC GTCCACGCCC AGGTGACCTC 
TGCCCACCCT CGAAGGATGG GGATGCATCA GGCCCAAGGG CTACCAATGA CTTGGTTCTG 
CGCCGGGCGC GGCACCAGCA GATGTCAGGG GATGTGGCAG TAGAGAAGAG GCCTTCTCGA 
ACTGGGGGCA AAGTCATCAA ATCAGCCTCA GCCACTGCCT TATCTGTCAT GATTCCTGCA 
GTGGACCCAC ATGGAAGTTC ACCCCTTGCT AGTCCCATGT CTCCACGATC TCTGTCCTCC 
AACCCATCCT CACGGGACTC CTCACCCAGC CGGGACTACT CACCAGCTGT CAGTGGGCTC 
CGCTCCCCCA TCACCATCCA GCGCTCGGGC AAGAAGTATG GCTTCACACT GCGTGCCATC 
CGTGTCTACA TGGGTGACAC GGATGTCTAT AGTGTCCACC ACATTGTCTG GCATGTGGAG 
GAAGGAGGCC CAGCCCAGGA GGCAGGACTC TGTGCTGGGG ACCTCATCAC CCACGTGAAT 
GGGGAGCCTG TGCATGGCAT GGTGCATCCT GAGGTCGTGG AGCTGATCCT TAAGAGTGGC 
AACAAGGTAG CAGTGACCAC AACGCCCTTC GAAAATACCT CTATCCGCAT TGGTCCCGCA 
AGGCGCAGCA GCTACAAGGC TAAAATGGCT CGGAGGAACA AGCGACCCTC CGCCAAGGAG 
GGCCAGGAGA GCAAGAAGCG CAGCTCCCTC TTCCGGAAGA TCACGAAGCA GTCGAACCTG 
CTGCATACTA GCCGCTCGCT GTCGTCGCTG AACCGCTCGC TGTCATCCAG CGATAGTCTC 
CCGGGCTCGC CTACGCACGG GCTGCCGGCG CGCTCGCCCA CGCACAGCTA CCGCTCCACG 
CCTGACTCCG CCTACCTAGG CGCCTCATCC CAGAGCAGCT CCCCAGCCTC GAGCACGCCC 
AACTCGCCTG CGTCGTCGGC GTCGCACCAC ATTCGGCCCA GCACGCTGCA CGGACTGTCG 
CCAAAGCTCC ATCGCCAGTA CCGCTCTGCG CGATGCAAGT CGGCCGGCAA CATCCCTCTA 
TCGCCGCTGG CACACACGCC GTCCCCCACG CAGGCGTCAC CGCCGCCACT GCCGGGCCAC 
ACGGTGGGCA GCTCGCACAC TACTCAGAGC TTCCCGGCCA AACTGCACTC ATCGCCTCCC 
GTCGTGCGCC CGCGCCCCAA GAGTGCCGAG CCCCCTCGCT CGCCGCTCCT CAAGCGCGTG 
CAGTCGGCCG AGAAGCTGGG AGCCTCTTTG AGTGCGGACA AGAAGGGCGC GCTGCGCAAA 
CACAGCCTCG AGGTGGGCCA CCCGGATTTC CGCAAGGACT TCCATGGCGA GCTGGCGCTG 
CATAGCCTTG CCGAGTCCGA CGGTGAGACG CCCCCAGTCG AGGGCCTTGG CGCGCCCCGG 
CAGGTCGCCG TCCGCCGCCT GGGCCGACAG GAGTCACCTT TGAGCCTGGG CGCGGACCCG 
TTGCTGCCCG AGGGTGCCTC CAGGCCACCA GTGTCGAGCA AGGAGAAGGA ATCCCCGGGG 
GGCGCCGAGG CGTGCACCCC ACCCCGCGCG ACGACCCCCG GTGGCCGGAC CCTGGAGCGG 
GACGTCGGCT GCACGCGGCA TCAGAGCGTG CAGACGGAGG ATGGCACTGG CGGGATGGCC 
AGGGCTGTGG CCAAGGCGGC GCTGAGCCCG GTGCAGGAAC ACGAGACAGG CCGGCGCAGC 
AGCTCTGGCG AGGCGGGCAC ACCCCTGGTA CCCATTGTCG TAGAGCCTGC GCGGCCCGGG 
GCTAAGGCTG TGGTGCCTCA GCCTCTGGGC GCGGACTCCA AGGGGTTGCA GGAACCCGCA 
CCCCTGGCGC CTTCCGTGCC CGAGGCCCCC CGGGGCCGGG AGCGCTGGGT GTTGGAGGTG 
GTGGAGGAGC GCACCACGCT GAGCGGTCCT CGCTCCAAGC CCGCCTCCCC AAAGCTCTCC 
CCGGAGCCCC AGACACCCTC CCTAGCCCCA GCGAAGTGCA GTGCACCCAG CAGTGCAGTG 
ACCCCAGTCC CACCCGCATC CCTCTTGGGC TCAGGCACCA AGCCTCAAGT GGGGCTGACC 
TCCCGGTGCC CTGCTGAAGC TGTGCCCCCA GCAGGCCTGA CCAAAAAAGG AGTGTCCAGT 
CCCGCACCCC CGGGACCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526110.1
CDS	300..3680
Translation	MALDFILNATEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVAAAGSSKREPSTKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFSAPQEDEDEARLRRPPRPSSDPAGSLDARAPKEETQGEGTSSAGDSEATDRPRPGDLCPPSKDGDASGPRATNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLSADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPVEGLGAPRQVAVRRLGRQESPLSLGADPLLPEGASRPPVSSKEKESPGGAEACTPPRATTPGGRTLERDVGCTRHQSVQTEDGTGGMARAVAKAALSPVQEHETGRRSSSGEAGTPLVPIVVEPARPGAKAVVPQPLGADSKGLQEPAPLAPSVPEAPRGRERWVLEVVEERTTLSGPRSKPASPKLSPEPQTPSLAPAKCSAPSSAVTPVPPASLLGSGTKPQVGLTSRCPAEAVPPAGLTKKGVSSPAPPGP

Target ORF information:

RefSeq Version	XM_011527808.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527808.2

ORF Insert Sequence:

ATGGCATTGG ATTTCATTCT GAATGCAACA GAAGGCGGCG ACTGTGCCAC CCTGCTGAAG 
AATATTGGAG CGCTGCCCGT AGAGATGGCC CGCATGTACT TTGCTGAGAC GGTGCTAGCC 
CTGGAGTATT TGCACAACTA TGGCATCGTG CACCGCGACC TCAAGCCTGA CAACCTCCTT 
ATCACCTCCA TGGGTCACAT CAAGCTCACA GATTTCGGCC TCTCCAAGAT GGGGCTCATG 
AGCCTCACCA CCAACTTATA TGAAGGCCAC ATCGAGAAGG ACGCCCGAGA GTTCCTGGAC 
AAACAGGTGT GTGGGACCCC AGAGTACATC GCGCCCGAGG TCATCCTGCG TCAAGGCTAC 
GGCAAGCCAG TGGACTGGTG GGCTATGGGG ATCATCCTCT ACGAGTTCCT GGTGGGCTGT 
GTGCCCTTCT TCGGAGACAC ACCAGAGGAG CTATTTGGAC AGGTCATCAG TGATGACATC 
CTGTGGCCCG AGGGGGATGA GGCCCTACCT ACGGAGGCCC AACTCCTCAT ATCCAGCCTC 
CTGCAGACCA ACCCTCTGGT CAGGCTTGGG GCAGGCGGCG CTTTTGAGGT GAAGCAGCAC 
AGTTTCTTTC GAGACCTGGA CTGGACAGGG CTGCTGAGGC AGAAGGCCGA GTTCATCCCC 
CACCTAGAGT CGGAAGATGA CACTAGCTAC TTTGACACCC GCTCAGACAG GTATCACCAC 
GTGAACTCCT ATGACGAGGA TGACACGACG GAGGAGGAGC CCGTGGAAAT CCGCCAGTTC 
TCTTCCTGCT CTCCGCGCTT CAGCAAGGTG TATAGCAGCA TGGAGCAGCT GTCGCAGCAC 
GAGCCCAAGA CCCCAGTAGC AGCTGCAGGG AGCAGCAAGC GGGAGCCGAG CACCAAGGGC 
CCCGAGGAGA AGGTGGCCGG CAAGCGGGAG GGGCTGGGCG GCCTGACCCT GCGTGAGAAG 
ACCTGGAGAG GGGGCTCTCC GGAGATCAAG CGATTCTCCG CGTCCGAGGC CAGTTTCCTG 
GAGGGAGAGG CCAGTCCCCC TTTGGGCGCC CGCCGCCGTT TCTCGGCGCT GCTGGAGCCC 
AGCCGCTTCA GCGCCCCCCA AGAGGACGAG GATGAGGCCC GGCTGCGCAG GCCTCCCCGG 
CCCAGCTCCG ACCCCGCGGG ATCCCTGGAT GCACGGGCCC CCAAAGAGGA GACTCAAGGG 
GAAGGCACCT CCAGCGCCGG GGACTCCGAG GCCACTGACC GTCCACGCCC AGGTGACCTC 
TGCCCACCCT CGAAGGATGG GGATGCATCA GGCCCAAGGG CTACCAATGA CTTGGTTCTG 
CGCCGGGCGC GGCACCAGCA GATGTCAGGG GATGTGGCAG TAGAGAAGAG GCCTTCTCGA 
ACTGGGGGCA AAGTCATCAA ATCAGCCTCA GCCACTGCCT TATCTGTCAT GATTCCTGCA 
GTGGACCCAC ATGGAAGTTC ACCCCTTGCT AGTCCCATGT CTCCACGATC TCTGTCCTCC 
AACCCATCCT CACGGGACTC CTCACCCAGC CGGGACTACT CACCAGCTGT CAGTGGGCTC 
CGCTCCCCCA TCACCATCCA GCGCTCGGGC AAGAAGTATG GCTTCACACT GCGTGCCATC 
CGTGTCTACA TGGGTGACAC GGATGTCTAT AGTGTCCACC ACATTGTCTG GCATGTGGAG 
GAAGGAGGCC CAGCCCAGGA GGCAGGACTC TGTGCTGGGG ACCTCATCAC CCACGTGAAT 
GGGGAGCCTG TGCATGGCAT GGTGCATCCT GAGGTCGTGG AGCTGATCCT TAAGAGTGGC 
AACAAGGTAG CAGTGACCAC AACGCCCTTC GAAAATACCT CTATCCGCAT TGGTCCCGCA 
AGGCGCAGCA GCTACAAGGC TAAAATGGCT CGGAGGAACA AGCGACCCTC CGCCAAGGAG 
GGCCAGGAGA GCAAGAAGCG CAGCTCCCTC TTCCGGAAGA TCACGAAGCA GTCGAACCTG 
CTGCATACTA GCCGCTCGCT GTCGTCGCTG AACCGCTCGC TGTCATCCAG CGATAGTCTC 
CCGGGCTCGC CTACGCACGG GCTGCCGGCG CGCTCGCCCA CGCACAGCTA CCGCTCCACG 
CCTGACTCCG CCTACCTAGG CGCCTCATCC CAGAGCAGCT CCCCAGCCTC GAGCACGCCC 
AACTCGCCTG CGTCGTCGGC GTCGCACCAC ATTCGGCCCA GCACGCTGCA CGGACTGTCG 
CCAAAGCTCC ATCGCCAGTA CCGCTCTGCG CGATGCAAGT CGGCCGGCAA CATCCCTCTA 
TCGCCGCTGG CACACACGCC GTCCCCCACG CAGGCGTCAC CGCCGCCACT GCCGGGCCAC 
ACGGTGGGCA GCTCGCACAC TACTCAGAGC TTCCCGGCCA AACTGCACTC ATCGCCTCCC 
GTCGTGCGCC CGCGCCCCAA GAGTGCCGAG CCCCCTCGCT CGCCGCTCCT CAAGCGCGTG 
CAGTCGGCCG AGAAGCTGGG AGCCTCTTTG AGTGCGGACA AGAAGGGCGC GCTGCGCAAA 
CACAGCCTCG AGGTGGGCCA CCCGGATTTC CGCAAGGACT TCCATGGCGA GCTGGCGCTG 
CATAGCCTTG CCGAGTCCGA CGGTGAGACG CCCCCAGTCG AGGGCCTTGG CGCGCCCCGG 
CAGGTCGCCG TCCGCCGCCT GGGCCGACAG GAGTCACCTT TGAGCCTGGG CGCGGACCCG 
TTGCTGCCCG AGGGTGCCTC CAGGCCACCA GTGTCGAGCA AGGAGAAGGA ATCCCCGGGG 
GGCGCCGAGG CGTGCACCCC ACCCCGCGCG ACGACCCCCG GTGGCCGGAC CCTGGAGCGG 
GACGTCGGCT GCACGCGGCA TCAGAGCGTG CAGACGGAGG ATGGCACTGG CGGGATGGCC 
AGGGCTGTGG CCAAGGCGGC GCTGAGCCCG GTGCAGGAAC ACGAGACAGG CCGGCGCAGC 
AGCTCTGGCG AGGCGGGCAC ACCCCTGGTA CCCATTGTCG TAGAGCCTGC GCGGCCCGGG 
GCTAAGGCTG TGGTGCCTCA GCCTCTGGGC GCGGACTCCA AGGGGTTGCA GGAACCCGCA 
CCCCTGGCGC CTTCCGTGCC CGAGGCCCCC CGGGGCCGGG AGCGCTGGGT GTTGGAGGTG 
GTGGAGGAGC GCACCACGCT GAGCGGTCCT CGCTCCAAGC CCGCCTCCCC AAAGCTCTCC 
CCGGAGCCCC AGACACCCTC CCTAGCCCCA GCGAAGTGCA GTGCACCCAG CAGTGCAGTG 
ACCCCAGTCC CACCCGCATC CCTCTTGGGC TCAGGCACCA AGCCTCAAGT GGGGCTGACC 
TCCCGGTGCC CTGCTGAAGC TGTGCCCCCA GCAGGCCTGA CCAAAAAAGG AGTGTCCAGT 
CCCGCACCCC CGGGACCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST1 ( XM_017026501.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881990.1 Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.

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CloneID	OHu88317
Clone ID Related Accession (Same CDS sequence)	XM_017026501.1
Accession Version	XM_017026501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGA TCAACAAGCA GAACTTGATC CTCCGCAACC AGATCCAGCA GGCCTTTGTG 
GAGCGCGATA TCCTCACCTT CGCCGAGAAC CCGTTTGTGG TCGGCATGTT CTGCTCCTTT 
GAGACTCGGC GCCACCTCTG CATGGTCATG GAATATGTGG AAGGCGGCGA CTGTGCCACC 
CTGCTGAAGA ATATTGGAGC GCTGCCCGTA GAGATGGCCC GCATGTACTT TGCTGAGACG 
GTGCTAGCCC TGGAGTATTT GCACAACTAT GGCATCGTGC ACCGCGACCT CAAGCCTGAC 
AACCTCCTTA TCACCTCCAT GGGTCACATC AAGCTCACAG ATTTCGGCCT CTCCAAGATG 
GGGCTCATGA GCCTCACCAC CAACTTATAT GAAGGCCACA TCGAGAAGGA CGCCCGAGAG 
TTCCTGGACA AACAGGTGTG TGGGACCCCA GAGTACATCG CGCCCGAGGT CATCCTGCGT 
CAAGGCTACG GCAAGCCAGT GGACTGGTGG GCTATGGGGA TCATCCTCTA CGAGTTCCTG 
GTGGGCTGTG TGCCCTTCTT CGGAGACACA CCAGAGGAGC TATTTGGACA GGTCATCAGT 
GATGACATCC TGTGGCCCGA GGGGGATGAG GCCCTACCTA CGGAGGCCCA ACTCCTCATA 
TCCAGCCTCC TGCAGACCAA CCCTCTGGTC AGGCTTGGGG CAGGCGGCGC TTTTGAGGTG 
AAGCAGCACA GTTTCTTTCG AGACCTGGAC TGGACAGGGC TGCTGAGGCA GAAGGCCGAG 
TTCATCCCCC ACCTAGAGTC GGAAGATGAC ACTAGCTACT TTGACACCCG CTCAGACAGG 
TATCACCACG TGAACTCCTA TGACGAGGAT GACACGACGG AGGAGGAGCC CGTGGAAATC 
CGCCAGTTCT CTTCCTGCTC TCCGCGCTTC AGCAAGGTGT ATAGCAGCAT GGAGCAGCTG 
TCGCAGCACG AGCCCAAGAC CCCAGTAGCA GCTGCAGGGA GCAGCAAGCG GGAGCCGAGC 
ACCAAGGGCC CCGAGGAGAA GGTGGCCGGC AAGCGGGAGG GGCTGGGCGG CCTGACCCTG 
CGTGAGAAGA CCTGGAGAGG GGGCTCTCCG GAGATCAAGC GATTCTCCGC GTCCGAGGCC 
AGTTTCCTGG AGGGAGAGGC CAGTCCCCCT TTGGGCGCCC GCCGCCGTTT CTCGGCGCTG 
CTGGAGCCCA GCCGCTTCAG CGCCCCCCAA GAGGACGAGG ATGAGGCCCG GCTGCGCAGG 
CCTCCCCGGC CCAGCTCCGA CCCCGCGGGA TCCCTGGATG CACGGGCCCC CAAAGAGGAG 
ACTCAAGGGG AAGGCACCTC CAGCGCCGGG GACTCCGAGG CCACTGACCG TCCACGCCCA 
GGTGACCTCT GCCCACCCTC GAAGGATGGG GATGCATCAG GCCCAAGGGC TACCAATGAC 
TTGGTTCTGC GCCGGGCGCG GCACCAGCAG ATGTCAGGGG ATGTGGCAGT AGAGAAGAGG 
CCTTCTCGAA CTGGGGGCAA AGTCATCAAA TCAGCCTCAG CCACTGCCTT ATCTGTCATG 
ATTCCTGCAG TGGACCCACA TGGAAGTTCA CCCCTTGCTA GTCCCATGTC TCCACGATCT 
CTGTCCTCCA ACCCATCCTC ACGGGACTCC TCACCCAGCC GGGACTACTC ACCAGCTGTC 
AGTGGGCTCC GCTCCCCCAT CACCATCCAG CGCTCGGGCA AGAAGTATGG CTTCACACTG 
CGTGCCATCC GTGTCTACAT GGGTGACACG GATGTCTATA GTGTCCACCA CATTGTCTGG 
CATGTGGAGG AAGGAGGCCC AGCCCAGGAG GCAGGACTCT GTGCTGGGGA CCTCATCACC 
CACGTGAATG GGGAGCCTGT GCATGGCATG GTGCATCCTG AGGTCGTGGA GCTGATCCTT 
AAGAGTGGCA ACAAGGTAGC AGTGACCACA ACGCCCTTCG AAAATACCTC TATCCGCATT 
GGTCCCGCAA GGCGCAGCAG CTACAAGGCT AAAATGGCTC GGAGGAACAA GCGACCCTCC 
GCCAAGGAGG GCCAGGAGAG CAAGAAGCGC AGCTCCCTCT TCCGGAAGAT CACGAAGCAG 
TCGAACCTGC TGCATACTAG CCGCTCGCTG TCGTCGCTGA ACCGCTCGCT GTCATCCAGC 
GATAGTCTCC CGGGCTCGCC TACGCACGGG CTGCCGGCGC GCTCGCCCAC GCACAGCTAC 
CGCTCCACGC CTGACTCCGC CTACCTAGGC GCCTCATCCC AGAGCAGCTC CCCAGCCTCG 
AGCACGCCCA ACTCGCCTGC GTCGTCGGCG TCGCACCACA TTCGGCCCAG CACGCTGCAC 
GGACTGTCGC CAAAGCTCCA TCGCCAGTAC CGCTCTGCGC GATGCAAGTC GGCCGGCAAC 
ATCCCTCTAT CGCCGCTGGC ACACACGCCG TCCCCCACGC AGGCGTCACC GCCGCCACTG 
CCGGGCCACA CGGTGGGCAG CTCGCACACT ACTCAGAGCT TCCCGGCCAA ACTGCACTCA 
TCGCCTCCCG TCGTGCGCCC GCGCCCCAAG AGTGCCGAGC CCCCTCGCTC GCCGCTCCTC 
AAGCGCGTGC AGTCGGCCGA GAAGCTGGGA GCCTCTTTGA GTGCGGACAA GAAGGGCGCG 
CTGCGCAAAC ACAGCCTCGA GGTGGGCCAC CCGGATTTCC GCAAGGACTT CCATGGCGAG 
CTGGCGCTGC ATAGCCTTGC CGAGTCCGAC GGTGAGACGC CCCCAGTCGA GGGCCTTGGC 
GCGCCCCGGC AGGTCGCCGT CCGCCGCCTG GGCCGACAGG AGTCACCTTT GAGCCTGGGC 
GCGGACCCGT TGCTGCCCGA GGGTGCCTCC AGGCCACCAG TGTCGAGCAA GGAGAAGGAA 
TCCCCGGGGG GCGCCGAGGC GTGCACCCCA CCCCGCGCGA CGACCCCCGG TGGCCGGACC 
CTGGAGCGGG ACGTCGGCTG CACGCGGCAT CAGAGCGTGC AGACGGAGGA TGGCACTGGC 
GGGATGGCCA GGGCTGTGGC CAAGGCGGCG CTGAGCCCGG TGCAGGAACA CGAGACAGGC 
CGGCGCAGCA GCTCTGGCGA GGCGGGCACA CCCCTGGTAC CCATTGTCGT AGAGCCTGCG 
CGGCCCGGGG CTAAGGCTGT GGTGCCTCAG CCTCTGGGCG CGGACTCCAA GGGGTTGCAG 
GAACCCGCAC CCCTGGCGCC TTCCGTGCCC GAGGCCCCCC GGGGCCGGGA GCGCTGGGTG 
TTGGAGGTGG TGGAGGAGCG CACCACGCTG AGCGGTCCTC GCTCCAAGCC CGCCTCCCCA 
AAGCTCTCCC CGGAGCCCCA GACACCCTCC CTAGCCCCAG CGAAGTGCAG TGCACCCAGC 
AGTGCAGTGA CCCCAGTCCC ACCCGCATCC CTCTTGGGCT CAGGCACCAA GCCTCAAGTG 
GGGCTGACCT CCCGGTGCCC TGCTGAAGCT GTGCCCCCAG CAGGCCTGAC CAAAAAAGGA 
GTGTCCAGTC CCGCACCCCC GGGACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881990.1
CDS	785..4294
Translation	MKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVAAAGSSKREPSTKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFSAPQEDEDEARLRRPPRPSSDPAGSLDARAPKEETQGEGTSSAGDSEATDRPRPGDLCPPSKDGDASGPRATNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLSADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPVEGLGAPRQVAVRRLGRQESPLSLGADPLLPEGASRPPVSSKEKESPGGAEACTPPRATTPGGRTLERDVGCTRHQSVQTEDGTGGMARAVAKAALSPVQEHETGRRSSSGEAGTPLVPIVVEPARPGAKAVVPQPLGADSKGLQEPAPLAPSVPEAPRGRERWVLEVVEERTTLSGPRSKPASPKLSPEPQTPSLAPAKCSAPSSAVTPVPPASLLGSGTKPQVGLTSRCPAEAVPPAGLTKKGVSSPAPPGP

Target ORF information:

RefSeq Version	XM_017026501.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026501.1

ORF Insert Sequence:

ATGAAAAAGA TCAACAAGCA GAACTTGATC CTCCGCAACC AGATCCAGCA GGCCTTTGTG 
GAGCGCGATA TCCTCACCTT CGCCGAGAAC CCGTTTGTGG TCGGCATGTT CTGCTCCTTT 
GAGACTCGGC GCCACCTCTG CATGGTCATG GAATATGTGG AAGGCGGCGA CTGTGCCACC 
CTGCTGAAGA ATATTGGAGC GCTGCCCGTA GAGATGGCCC GCATGTACTT TGCTGAGACG 
GTGCTAGCCC TGGAGTATTT GCACAACTAT GGCATCGTGC ACCGCGACCT CAAGCCTGAC 
AACCTCCTTA TCACCTCCAT GGGTCACATC AAGCTCACAG ATTTCGGCCT CTCCAAGATG 
GGGCTCATGA GCCTCACCAC CAACTTATAT GAAGGCCACA TCGAGAAGGA CGCCCGAGAG 
TTCCTGGACA AACAGGTGTG TGGGACCCCA GAGTACATCG CGCCCGAGGT CATCCTGCGT 
CAAGGCTACG GCAAGCCAGT GGACTGGTGG GCTATGGGGA TCATCCTCTA CGAGTTCCTG 
GTGGGCTGTG TGCCCTTCTT CGGAGACACA CCAGAGGAGC TATTTGGACA GGTCATCAGT 
GATGACATCC TGTGGCCCGA GGGGGATGAG GCCCTACCTA CGGAGGCCCA ACTCCTCATA 
TCCAGCCTCC TGCAGACCAA CCCTCTGGTC AGGCTTGGGG CAGGCGGCGC TTTTGAGGTG 
AAGCAGCACA GTTTCTTTCG AGACCTGGAC TGGACAGGGC TGCTGAGGCA GAAGGCCGAG 
TTCATCCCCC ACCTAGAGTC GGAAGATGAC ACTAGCTACT TTGACACCCG CTCAGACAGG 
TATCACCACG TGAACTCCTA TGACGAGGAT GACACGACGG AGGAGGAGCC CGTGGAAATC 
CGCCAGTTCT CTTCCTGCTC TCCGCGCTTC AGCAAGGTGT ATAGCAGCAT GGAGCAGCTG 
TCGCAGCACG AGCCCAAGAC CCCAGTAGCA GCTGCAGGGA GCAGCAAGCG GGAGCCGAGC 
ACCAAGGGCC CCGAGGAGAA GGTGGCCGGC AAGCGGGAGG GGCTGGGCGG CCTGACCCTG 
CGTGAGAAGA CCTGGAGAGG GGGCTCTCCG GAGATCAAGC GATTCTCCGC GTCCGAGGCC 
AGTTTCCTGG AGGGAGAGGC CAGTCCCCCT TTGGGCGCCC GCCGCCGTTT CTCGGCGCTG 
CTGGAGCCCA GCCGCTTCAG CGCCCCCCAA GAGGACGAGG ATGAGGCCCG GCTGCGCAGG 
CCTCCCCGGC CCAGCTCCGA CCCCGCGGGA TCCCTGGATG CACGGGCCCC CAAAGAGGAG 
ACTCAAGGGG AAGGCACCTC CAGCGCCGGG GACTCCGAGG CCACTGACCG TCCACGCCCA 
GGTGACCTCT GCCCACCCTC GAAGGATGGG GATGCATCAG GCCCAAGGGC TACCAATGAC 
TTGGTTCTGC GCCGGGCGCG GCACCAGCAG ATGTCAGGGG ATGTGGCAGT AGAGAAGAGG 
CCTTCTCGAA CTGGGGGCAA AGTCATCAAA TCAGCCTCAG CCACTGCCTT ATCTGTCATG 
ATTCCTGCAG TGGACCCACA TGGAAGTTCA CCCCTTGCTA GTCCCATGTC TCCACGATCT 
CTGTCCTCCA ACCCATCCTC ACGGGACTCC TCACCCAGCC GGGACTACTC ACCAGCTGTC 
AGTGGGCTCC GCTCCCCCAT CACCATCCAG CGCTCGGGCA AGAAGTATGG CTTCACACTG 
CGTGCCATCC GTGTCTACAT GGGTGACACG GATGTCTATA GTGTCCACCA CATTGTCTGG 
CATGTGGAGG AAGGAGGCCC AGCCCAGGAG GCAGGACTCT GTGCTGGGGA CCTCATCACC 
CACGTGAATG GGGAGCCTGT GCATGGCATG GTGCATCCTG AGGTCGTGGA GCTGATCCTT 
AAGAGTGGCA ACAAGGTAGC AGTGACCACA ACGCCCTTCG AAAATACCTC TATCCGCATT 
GGTCCCGCAA GGCGCAGCAG CTACAAGGCT AAAATGGCTC GGAGGAACAA GCGACCCTCC 
GCCAAGGAGG GCCAGGAGAG CAAGAAGCGC AGCTCCCTCT TCCGGAAGAT CACGAAGCAG 
TCGAACCTGC TGCATACTAG CCGCTCGCTG TCGTCGCTGA ACCGCTCGCT GTCATCCAGC 
GATAGTCTCC CGGGCTCGCC TACGCACGGG CTGCCGGCGC GCTCGCCCAC GCACAGCTAC 
CGCTCCACGC CTGACTCCGC CTACCTAGGC GCCTCATCCC AGAGCAGCTC CCCAGCCTCG 
AGCACGCCCA ACTCGCCTGC GTCGTCGGCG TCGCACCACA TTCGGCCCAG CACGCTGCAC 
GGACTGTCGC CAAAGCTCCA TCGCCAGTAC CGCTCTGCGC GATGCAAGTC GGCCGGCAAC 
ATCCCTCTAT CGCCGCTGGC ACACACGCCG TCCCCCACGC AGGCGTCACC GCCGCCACTG 
CCGGGCCACA CGGTGGGCAG CTCGCACACT ACTCAGAGCT TCCCGGCCAA ACTGCACTCA 
TCGCCTCCCG TCGTGCGCCC GCGCCCCAAG AGTGCCGAGC CCCCTCGCTC GCCGCTCCTC 
AAGCGCGTGC AGTCGGCCGA GAAGCTGGGA GCCTCTTTGA GTGCGGACAA GAAGGGCGCG 
CTGCGCAAAC ACAGCCTCGA GGTGGGCCAC CCGGATTTCC GCAAGGACTT CCATGGCGAG 
CTGGCGCTGC ATAGCCTTGC CGAGTCCGAC GGTGAGACGC CCCCAGTCGA GGGCCTTGGC 
GCGCCCCGGC AGGTCGCCGT CCGCCGCCTG GGCCGACAGG AGTCACCTTT GAGCCTGGGC 
GCGGACCCGT TGCTGCCCGA GGGTGCCTCC AGGCCACCAG TGTCGAGCAA GGAGAAGGAA 
TCCCCGGGGG GCGCCGAGGC GTGCACCCCA CCCCGCGCGA CGACCCCCGG TGGCCGGACC 
CTGGAGCGGG ACGTCGGCTG CACGCGGCAT CAGAGCGTGC AGACGGAGGA TGGCACTGGC 
GGGATGGCCA GGGCTGTGGC CAAGGCGGCG CTGAGCCCGG TGCAGGAACA CGAGACAGGC 
CGGCGCAGCA GCTCTGGCGA GGCGGGCACA CCCCTGGTAC CCATTGTCGT AGAGCCTGCG 
CGGCCCGGGG CTAAGGCTGT GGTGCCTCAG CCTCTGGGCG CGGACTCCAA GGGGTTGCAG 
GAACCCGCAC CCCTGGCGCC TTCCGTGCCC GAGGCCCCCC GGGGCCGGGA GCGCTGGGTG 
TTGGAGGTGG TGGAGGAGCG CACCACGCTG AGCGGTCCTC GCTCCAAGCC CGCCTCCCCA 
AAGCTCTCCC CGGAGCCCCA GACACCCTCC CTAGCCCCAG CGAAGTGCAG TGCACCCAGC 
AGTGCAGTGA CCCCAGTCCC ACCCGCATCC CTCTTGGGCT CAGGCACCAA GCCTCAAGTG 
GGGCTGACCT CCCGGTGCCC TGCTGAAGCT GTGCCCCCAG CAGGCCTGAC CAAAAAAGGA 
GTGTCCAGTC CCGCACCCCC GGGACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functionally recurrent rearrangements of the MAST kinase and Notch gene families in breast cancer.
Nature medicine17(12)1646-51(2011 Nov)
Robinson DR,Kalyana-Sundaram S,Wu YM,Shankar S,Cao X,Ateeq B,Asangani IA,Iyer M,Maher CA,Grasso CS,Lonigro RJ,Quist M,Siddiqui J,Mehra R,Jing X,Giordano TJ,Sabel MS,Kleer CG,Palanisamy N,Natrajan R,Lambros MB,Reis-Filho JS,Kumar-Sinha C,Chinnaiyan AM

Regulation of epidermal growth factor receptor trafficking by lysine deacetylase HDAC6.
Science signaling2(102)ra84(2009 Dec)
Deribe YL,Wild P,Chandrashaker A,Curak J,Schmidt MH,Kalaidzidis Y,Milutinovic N,Kratchmarova I,Buerkle L,Fetchko MJ,Schmidt P,Kittanakom S,Brown KR,Jurisica I,Blagoev B,Zerial M,Stagljar I,Dikic I

Sast124, a novel splice variant of syntrophin-associated serine/threonine kinase (SAST), is specifically localized in the restricted brain regions.
Neuroscience117(2)373-81(2003)
Yano R,Yap CC,Yamazaki Y,Muto Y,Kishida H,Okada D,Hashikawa T

Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases.
Nature neuroscience2(7)611-7(1999 Jul)
Lumeng C,Phelps S,Crawford GE,Walden PD,Barald K,Chamberlain JS

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MAST1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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