NLRP1 cDNA ORF clone, Homo sapiens(human)

The following NLRP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25361 NM_033004.4
Latest version!
Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu25606 NM_001033053.3
Latest version!
Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu25373 NM_014922.5
Latest version!
Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu25556 NM_033007.4
Latest version!
Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu25557 NM_033006.4
Latest version!
Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
View Old Accession Versions >>
OHu25606 NM_001033053.2 Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu25557 NM_033006.3 Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu25556 NM_033007.3 Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu25373 NM_014922.4 Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu25361 NM_033004.3 Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25361
    Clone ID Related Accession (Same CDS sequence) NM_033004.3 , NM_033004.4
    Accession Version NM_033004.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229060.1, AB023143.1 and AI819334.1. On Dec 14, 2006 this sequence version replaced NM_033004.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229060.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGCCATAG ATGATCTAGA AATGAAATTC CAGTTTGTGC GAATCCACAA GCCACCCCCG
    CTGACCCCAC TTTATATGGG CTGTCGTTAC ACTGTGTCTG GGTCTGGTTC AGGGATGCTG
    GAAATACTCC CCAAGGAACT GGAGCTCTGC TATCGAAGCC CTGGAGAAGA CCAGCTGTTC
    TCGGAGTTCT ACGTTGGCCA CTTGGGATCA GGGATCAGGC TGCAAGTGAA AGACAAGAAA
    GATGAGACTC TGGTGTGGGA GGCCTTGGTG AAACCAGGAG ATCTCATGCC TGCAACTACT
    CTGATCCCTC CAGCCCGCAT AGCCGTACCT TCACCTCTGG ATGCCCCGCA GTTGCTGCAC
    TTTGTGGACC AGTATCGAGA GCAGCTGATA GCCCGAGTGA CATCGGTGGA GGTTGTCTTG
    GACAAACTGC ATGGACAGGT GCTGAGCCAG GAGCAGTACG AGAGGGTGCT GGCTGAGAAC
    ACGAGGCCCA GCCAGATGCG GAAGCTGTTC AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC
    AAAGATGGAC TCTACCAAGC CCTGAAGGAG ACCCATCCTC ACCTCATTAT GGAACTCTGG
    GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127497.1
    CDS556..4977
    Translation

    Target ORF information:

    RefSeq Version NM_033004.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033004.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGCCATAG ATGATCTAGA AATGAAATTC CAGTTTGTGC GAATCCACAA GCCACCCCCG
    CTGACCCCAC TTTATATGGG CTGTCGTTAC ACTGTGTCTG GGTCTGGTTC AGGGATGCTG
    GAAATACTCC CCAAGGAACT GGAGCTCTGC TATCGAAGCC CTGGAGAAGA CCAGCTGTTC
    TCGGAGTTCT ACGTTGGCCA CTTGGGATCA GGGATCAGGC TGCAAGTGAA AGACAAGAAA
    GATGAGACTC TGGTGTGGGA GGCCTTGGTG AAACCAGGAG ATCTCATGCC TGCAACTACT
    CTGATCCCTC CAGCCCGCAT AGCCGTACCT TCACCTCTGG ATGCCCCGCA GTTGCTGCAC
    TTTGTGGACC AGTATCGAGA GCAGCTGATA GCCCGAGTGA CATCGGTGGA GGTTGTCTTG
    GACAAACTGC ATGGACAGGT GCTGAGCCAG GAGCAGTACG AGAGGGTGCT GGCTGAGAAC
    ACGAGGCCCA GCCAGATGCG GAAGCTGTTC AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC
    AAAGATGGAC TCTACCAAGC CCTGAAGGAG ACCCATCCTC ACCTCATTAT GGAACTCTGG
    GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25361
    Clone ID Related Accession (Same CDS sequence) NM_033004.3 , NM_033004.4
    Accession Version NM_033004.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229060.1, AB023143.1 and AI819334.1. On Nov 22, 2018 this sequence version replaced NM_033004.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229060.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000572272.6/ ENSP00000460475.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGCCATAG ATGATCTAGA AATGAAATTC CAGTTTGTGC GAATCCACAA GCCACCCCCG
    CTGACCCCAC TTTATATGGG CTGTCGTTAC ACTGTGTCTG GGTCTGGTTC AGGGATGCTG
    GAAATACTCC CCAAGGAACT GGAGCTCTGC TATCGAAGCC CTGGAGAAGA CCAGCTGTTC
    TCGGAGTTCT ACGTTGGCCA CTTGGGATCA GGGATCAGGC TGCAAGTGAA AGACAAGAAA
    GATGAGACTC TGGTGTGGGA GGCCTTGGTG AAACCAGGAG ATCTCATGCC TGCAACTACT
    CTGATCCCTC CAGCCCGCAT AGCCGTACCT TCACCTCTGG ATGCCCCGCA GTTGCTGCAC
    TTTGTGGACC AGTATCGAGA GCAGCTGATA GCCCGAGTGA CATCGGTGGA GGTTGTCTTG
    GACAAACTGC ATGGACAGGT GCTGAGCCAG GAGCAGTACG AGAGGGTGCT GGCTGAGAAC
    ACGAGGCCCA GCCAGATGCG GAAGCTGTTC AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC
    AAAGATGGAC TCTACCAAGC CCTGAAGGAG ACCCATCCTC ACCTCATTAT GGAACTCTGG
    GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127497.1
    CDS553..4974
    Translation

    Target ORF information:

    RefSeq Version NM_033004.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033004.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGCCATAG ATGATCTAGA AATGAAATTC CAGTTTGTGC GAATCCACAA GCCACCCCCG
    CTGACCCCAC TTTATATGGG CTGTCGTTAC ACTGTGTCTG GGTCTGGTTC AGGGATGCTG
    GAAATACTCC CCAAGGAACT GGAGCTCTGC TATCGAAGCC CTGGAGAAGA CCAGCTGTTC
    TCGGAGTTCT ACGTTGGCCA CTTGGGATCA GGGATCAGGC TGCAAGTGAA AGACAAGAAA
    GATGAGACTC TGGTGTGGGA GGCCTTGGTG AAACCAGGAG ATCTCATGCC TGCAACTACT
    CTGATCCCTC CAGCCCGCAT AGCCGTACCT TCACCTCTGG ATGCCCCGCA GTTGCTGCAC
    TTTGTGGACC AGTATCGAGA GCAGCTGATA GCCCGAGTGA CATCGGTGGA GGTTGTCTTG
    GACAAACTGC ATGGACAGGT GCTGAGCCAG GAGCAGTACG AGAGGGTGCT GGCTGAGAAC
    ACGAGGCCCA GCCAGATGCG GAAGCTGTTC AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC
    AAAGATGGAC TCTACCAAGC CCTGAAGGAG ACCCATCCTC ACCTCATTAT GGAACTCTGG
    GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25606
    Clone ID Related Accession (Same CDS sequence) NM_001033053.2 , NM_001033053.3
    Accession Version NM_001033053.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AB023143.1, AK026398.1 and BP391071.1. This sequence is a reference standard in the RefSeqGene project. On or before Nov 1, 2008 this sequence version replaced XR_041053.1, NM_001033053.1. Transcript Variant: This variant (5) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (5) has a distinct and shorter C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC051787.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGGCAAGAG CCACGAGGAA AGCTCCCCAG AGGTAGTACC GGTGGAACTC
    TTGTGCGTGC CTTCTCCTGC CTCTCAAGGG GACCTGCATA CGAAGCCTTT GGGGACTGAC
    GATGACTTCT GGGGCCCCAC GGGGCCTGTG GCTACTGAGG TAGTTGACAA AGAAAAGAAC
    TTGTACCGAG TTCACTTCCC TGTAGCTGGC TCCTACCGCT GGCCCAACAC GGGTCTCTGC
    TTTGTGATGA GAGAAGCGGT GACCGTTGAG ATTGAATTCT GTGTGTGGGA CCAGTTCCTG
    GGTGAGATCA ACCCACAGCA CAGCTGGATG GTGGCAGGGC CTCTGCTGGA CATCAAGGCT
    GAGCCTGGAG CTGTGGAAGC TGTGCACCTC CCTCACTTTG TGGCTCTCCA AGGGGGCCAT
    GTGGACACAT CCCTGTTCCA AATGGCCCAC TTTAAAGAGG AGGGGATGCT CCTGGAGAAG
    CCAGCCAGGG TGGAGCTGCA TCACATAGTT CTGGAAAACC CCAGCTTCTC CCCCTTGGGA
    GTCCTCCTGA AAATGATCCA TAATGCCCTG CGCTTCATTC CCGTCACCTC TGTGGTGTTG
    CTTTACCACC GCGTCCATCC TGAGGAAGTC ACCTTCCACC TCTACCTGAT CCCAAGTGAC
    TGCTCCATTC GGAAGGCCAT AGATGATCTA GAAATGAAAT TCCAGTTTGT GCGAATCCAC
    AAGCCACCCC CGCTGACCCC ACTTTATATG GGCTGTCGTT ACACTGTGTC TGGGTCTGGT
    TCAGGGATGC TGGAAATACT CCCCAAGGAA CTGGAGCTCT GCTATCGAAG CCCTGGAGAA
    GACCAGCTGT TCTCGGAGTT CTACGTTGGC CACTTGGGAT CAGGGATCAG GCTGCAAGTG
    AAAGACAAGA AAGATGAGAC TCTGGTGTGG GAGGCCTTGG TGAAACCAGG AAGGAACACC
    AGCCAGCCGT GGAACCTCAG GTGCAACAGA GACGCCAGGA GATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028225.1
    CDS556..4683
    Translation

    Target ORF information:

    RefSeq Version NM_001033053.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033053.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGGCAAGAG CCACGAGGAA AGCTCCCCAG AGGTAGTACC GGTGGAACTC
    TTGTGCGTGC CTTCTCCTGC CTCTCAAGGG GACCTGCATA CGAAGCCTTT GGGGACTGAC
    GATGACTTCT GGGGCCCCAC GGGGCCTGTG GCTACTGAGG TAGTTGACAA AGAAAAGAAC
    TTGTACCGAG TTCACTTCCC TGTAGCTGGC TCCTACCGCT GGCCCAACAC GGGTCTCTGC
    TTTGTGATGA GAGAAGCGGT GACCGTTGAG ATTGAATTCT GTGTGTGGGA CCAGTTCCTG
    GGTGAGATCA ACCCACAGCA CAGCTGGATG GTGGCAGGGC CTCTGCTGGA CATCAAGGCT
    GAGCCTGGAG CTGTGGAAGC TGTGCACCTC CCTCACTTTG TGGCTCTCCA AGGGGGCCAT
    GTGGACACAT CCCTGTTCCA AATGGCCCAC TTTAAAGAGG AGGGGATGCT CCTGGAGAAG
    CCAGCCAGGG TGGAGCTGCA TCACATAGTT CTGGAAAACC CCAGCTTCTC CCCCTTGGGA
    GTCCTCCTGA AAATGATCCA TAATGCCCTG CGCTTCATTC CCGTCACCTC TGTGGTGTTG
    CTTTACCACC GCGTCCATCC TGAGGAAGTC ACCTTCCACC TCTACCTGAT CCCAAGTGAC
    TGCTCCATTC GGAAGGCCAT AGATGATCTA GAAATGAAAT TCCAGTTTGT GCGAATCCAC
    AAGCCACCCC CGCTGACCCC ACTTTATATG GGCTGTCGTT ACACTGTGTC TGGGTCTGGT
    TCAGGGATGC TGGAAATACT CCCCAAGGAA CTGGAGCTCT GCTATCGAAG CCCTGGAGAA
    GACCAGCTGT TCTCGGAGTT CTACGTTGGC CACTTGGGAT CAGGGATCAG GCTGCAAGTG
    AAAGACAAGA AAGATGAGAC TCTGGTGTGG GAGGCCTTGG TGAAACCAGG AAGGAACACC
    AGCCAGCCGT GGAACCTCAG GTGCAACAGA GACGCCAGGA GATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25606
    Clone ID Related Accession (Same CDS sequence) NM_001033053.2 , NM_001033053.3
    Accession Version NM_001033053.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AB023143.1, AK026398.1 and BP391071.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001033053.2. Transcript Variant: This variant (5) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (5) has a distinct and shorter C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGGCAAGAG CCACGAGGAA AGCTCCCCAG AGGTAGTACC GGTGGAACTC
    TTGTGCGTGC CTTCTCCTGC CTCTCAAGGG GACCTGCATA CGAAGCCTTT GGGGACTGAC
    GATGACTTCT GGGGCCCCAC GGGGCCTGTG GCTACTGAGG TAGTTGACAA AGAAAAGAAC
    TTGTACCGAG TTCACTTCCC TGTAGCTGGC TCCTACCGCT GGCCCAACAC GGGTCTCTGC
    TTTGTGATGA GAGAAGCGGT GACCGTTGAG ATTGAATTCT GTGTGTGGGA CCAGTTCCTG
    GGTGAGATCA ACCCACAGCA CAGCTGGATG GTGGCAGGGC CTCTGCTGGA CATCAAGGCT
    GAGCCTGGAG CTGTGGAAGC TGTGCACCTC CCTCACTTTG TGGCTCTCCA AGGGGGCCAT
    GTGGACACAT CCCTGTTCCA AATGGCCCAC TTTAAAGAGG AGGGGATGCT CCTGGAGAAG
    CCAGCCAGGG TGGAGCTGCA TCACATAGTT CTGGAAAACC CCAGCTTCTC CCCCTTGGGA
    GTCCTCCTGA AAATGATCCA TAATGCCCTG CGCTTCATTC CCGTCACCTC TGTGGTGTTG
    CTTTACCACC GCGTCCATCC TGAGGAAGTC ACCTTCCACC TCTACCTGAT CCCAAGTGAC
    TGCTCCATTC GGAAGGCCAT AGATGATCTA GAAATGAAAT TCCAGTTTGT GCGAATCCAC
    AAGCCACCCC CGCTGACCCC ACTTTATATG GGCTGTCGTT ACACTGTGTC TGGGTCTGGT
    TCAGGGATGC TGGAAATACT CCCCAAGGAA CTGGAGCTCT GCTATCGAAG CCCTGGAGAA
    GACCAGCTGT TCTCGGAGTT CTACGTTGGC CACTTGGGAT CAGGGATCAG GCTGCAAGTG
    AAAGACAAGA AAGATGAGAC TCTGGTGTGG GAGGCCTTGG TGAAACCAGG AAGGAACACC
    AGCCAGCCGT GGAACCTCAG GTGCAACAGA GACGCCAGGA GATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028225.1
    CDS553..4680
    Translation

    Target ORF information:

    RefSeq Version NM_001033053.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033053.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGGCAAGAG CCACGAGGAA AGCTCCCCAG AGGTAGTACC GGTGGAACTC
    TTGTGCGTGC CTTCTCCTGC CTCTCAAGGG GACCTGCATA CGAAGCCTTT GGGGACTGAC
    GATGACTTCT GGGGCCCCAC GGGGCCTGTG GCTACTGAGG TAGTTGACAA AGAAAAGAAC
    TTGTACCGAG TTCACTTCCC TGTAGCTGGC TCCTACCGCT GGCCCAACAC GGGTCTCTGC
    TTTGTGATGA GAGAAGCGGT GACCGTTGAG ATTGAATTCT GTGTGTGGGA CCAGTTCCTG
    GGTGAGATCA ACCCACAGCA CAGCTGGATG GTGGCAGGGC CTCTGCTGGA CATCAAGGCT
    GAGCCTGGAG CTGTGGAAGC TGTGCACCTC CCTCACTTTG TGGCTCTCCA AGGGGGCCAT
    GTGGACACAT CCCTGTTCCA AATGGCCCAC TTTAAAGAGG AGGGGATGCT CCTGGAGAAG
    CCAGCCAGGG TGGAGCTGCA TCACATAGTT CTGGAAAACC CCAGCTTCTC CCCCTTGGGA
    GTCCTCCTGA AAATGATCCA TAATGCCCTG CGCTTCATTC CCGTCACCTC TGTGGTGTTG
    CTTTACCACC GCGTCCATCC TGAGGAAGTC ACCTTCCACC TCTACCTGAT CCCAAGTGAC
    TGCTCCATTC GGAAGGCCAT AGATGATCTA GAAATGAAAT TCCAGTTTGT GCGAATCCAC
    AAGCCACCCC CGCTGACCCC ACTTTATATG GGCTGTCGTT ACACTGTGTC TGGGTCTGGT
    TCAGGGATGC TGGAAATACT CCCCAAGGAA CTGGAGCTCT GCTATCGAAG CCCTGGAGAA
    GACCAGCTGT TCTCGGAGTT CTACGTTGGC CACTTGGGAT CAGGGATCAG GCTGCAAGTG
    AAAGACAAGA AAGATGAGAC TCTGGTGTGG GAGGCCTTGG TGAAACCAGG AAGGAACACC
    AGCCAGCCGT GGAACCTCAG GTGCAACAGA GACGCCAGGA GATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25373
    Clone ID Related Accession (Same CDS sequence) NM_014922.5 , NM_014922.4
    Accession Version NM_014922.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AB023143.1 and AI819334.1. On May 31, 2019 this sequence version replaced NM_014922.4. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, as compared to variant 1. The encoded isoform 2 is missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB023143.1, AF310105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGAACTGG AGCTCTGCTA TCGAAGCCCT GGAGAAGACC AGCTGTTCTC GGAGTTCTAC
    GTTGGCCACT TGGGATCAGG GATCAGGCTG CAAGTGAAAG ACAAGAAAGA TGAGACTCTG
    GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT CTCATGCCTG CAACTACTCT GATCCCTCCA
    GCCCGCATAG CCGTACCTTC ACCTCTGGAT GCCCCGCAGT TGCTGCACTT TGTGGACCAG
    TATCGAGAGC AGCTGATAGC CCGAGTGACA TCGGTGGAGG TTGTCTTGGA CAAACTGCAT
    GGACAGGTGC TGAGCCAGGA GCAGTACGAG AGGGTGCTGG CTGAGAACAC GAGGCCCAGC
    CAGATGCGGA AGCTGTTCAG CTTGAGCCAG TCCTGGGACC GGAAGTGCAA AGATGGACTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTCATTATGG AACTCTGGGA GAAGGGCAGC
    AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055737.1
    CDS553..4842
    Translation

    Target ORF information:

    RefSeq Version NM_014922.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014922.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGAACTGG AGCTCTGCTA TCGAAGCCCT GGAGAAGACC AGCTGTTCTC GGAGTTCTAC
    GTTGGCCACT TGGGATCAGG GATCAGGCTG CAAGTGAAAG ACAAGAAAGA TGAGACTCTG
    GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT CTCATGCCTG CAACTACTCT GATCCCTCCA
    GCCCGCATAG CCGTACCTTC ACCTCTGGAT GCCCCGCAGT TGCTGCACTT TGTGGACCAG
    TATCGAGAGC AGCTGATAGC CCGAGTGACA TCGGTGGAGG TTGTCTTGGA CAAACTGCAT
    GGACAGGTGC TGAGCCAGGA GCAGTACGAG AGGGTGCTGG CTGAGAACAC GAGGCCCAGC
    CAGATGCGGA AGCTGTTCAG CTTGAGCCAG TCCTGGGACC GGAAGTGCAA AGATGGACTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTCATTATGG AACTCTGGGA GAAGGGCAGC
    AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25373
    Clone ID Related Accession (Same CDS sequence) NM_014922.5 , NM_014922.4
    Accession Version NM_014922.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AB023143.1 and AI819334.1. On Dec 14, 2006 this sequence version replaced NM_014922.3. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, as compared to variant 1. The encoded isoform 2 is missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB023143.1, AF310105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGAACTGG AGCTCTGCTA TCGAAGCCCT GGAGAAGACC AGCTGTTCTC GGAGTTCTAC
    GTTGGCCACT TGGGATCAGG GATCAGGCTG CAAGTGAAAG ACAAGAAAGA TGAGACTCTG
    GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT CTCATGCCTG CAACTACTCT GATCCCTCCA
    GCCCGCATAG CCGTACCTTC ACCTCTGGAT GCCCCGCAGT TGCTGCACTT TGTGGACCAG
    TATCGAGAGC AGCTGATAGC CCGAGTGACA TCGGTGGAGG TTGTCTTGGA CAAACTGCAT
    GGACAGGTGC TGAGCCAGGA GCAGTACGAG AGGGTGCTGG CTGAGAACAC GAGGCCCAGC
    CAGATGCGGA AGCTGTTCAG CTTGAGCCAG TCCTGGGACC GGAAGTGCAA AGATGGACTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTCATTATGG AACTCTGGGA GAAGGGCAGC
    AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055737.1
    CDS556..4845
    Translation

    Target ORF information:

    RefSeq Version NM_014922.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014922.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GCTGGACCAG
    ACAACTCTGA GTGATGAGAT GAGGCAGGAA CTGAGGGCCC TGGAGCAGGA GAAACCTCAG
    CTGCTCATCT TCAGCAGACG GAAACCAAGT GTGATGACCC CTACTGAGGG CCTGGATACG
    GGAGAGATGA GTAATAGCAC ATCCTCACTC AAGCGGCAGA GACTCGGATC AGAGAGGGCG
    GCTTCCCATG TTGCTCAGGC TAATCTCAAA CTCCTGGACG TGAGCAAGAT CTTCCCAATT
    GCTGAGATTG CAGAGGAAAG CTCCCCAGAG GTAGTACCGG TGGAACTCTT GTGCGTGCCT
    TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG GGACTGACGA TGACTTCTGG
    GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG AAAAGAACTT GTACCGAGTT
    CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG GTCTCTGCTT TGTGATGAGA
    GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC AGTTCCTGGG TGAGATCAAC
    CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA TCAAGGCTGA GCCTGGAGCT
    GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG GGGGCCATGT GGACACATCC
    CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA
    ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG TGGTGTTGCT TTACCACCGC
    GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC CAAGTGACTG CTCCATTCGG
    AAGGAACTGG AGCTCTGCTA TCGAAGCCCT GGAGAAGACC AGCTGTTCTC GGAGTTCTAC
    GTTGGCCACT TGGGATCAGG GATCAGGCTG CAAGTGAAAG ACAAGAAAGA TGAGACTCTG
    GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT CTCATGCCTG CAACTACTCT GATCCCTCCA
    GCCCGCATAG CCGTACCTTC ACCTCTGGAT GCCCCGCAGT TGCTGCACTT TGTGGACCAG
    TATCGAGAGC AGCTGATAGC CCGAGTGACA TCGGTGGAGG TTGTCTTGGA CAAACTGCAT
    GGACAGGTGC TGAGCCAGGA GCAGTACGAG AGGGTGCTGG CTGAGAACAC GAGGCCCAGC
    CAGATGCGGA AGCTGTTCAG CTTGAGCCAG TCCTGGGACC GGAAGTGCAA AGATGGACTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTCATTATGG AACTCTGGGA GAAGGGCAGC
    AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25556
    Clone ID Related Accession (Same CDS sequence) NM_033007.4 , NM_033007.3
    Accession Version NM_033007.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229061.1, AB023143.1 and AI819334.1. On May 31, 2019 this sequence version replaced NM_033007.3. Transcript Variant: This variant (4) lacks two in-frame exons in the coding region, as compared to variant 1. The encoded isoform 4 is missing two internal segments, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229061.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGAACTGG AGCTCTGCTA TCGAAGCCCT
    GGAGAAGACC AGCTGTTCTC GGAGTTCTAC GTTGGCCACT TGGGATCAGG GATCAGGCTG
    CAAGTGAAAG ACAAGAAAGA TGAGACTCTG GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT
    CTCATGCCTG CAACTACTCT GATCCCTCCA GCCCGCATAG CCGTACCTTC ACCTCTGGAT
    GCCCCGCAGT TGCTGCACTT TGTGGACCAG TATCGAGAGC AGCTGATAGC CCGAGTGACA
    TCGGTGGAGG TTGTCTTGGA CAAACTGCAT GGACAGGTGC TGAGCCAGGA GCAGTACGAG
    AGGGTGCTGG CTGAGAACAC GAGGCCCAGC CAGATGCGGA AGCTGTTCAG CTTGAGCCAG
    TCCTGGGACC GGAAGTGCAA AGATGGACTC TACCAAGCCC TGAAGGAGAC CCATCCTCAC
    CTCATTATGG AACTCTGGGA GAAGGGCAGC AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127500.1
    CDS553..4752
    Translation

    Target ORF information:

    RefSeq Version NM_033007.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033007.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGAACTGG AGCTCTGCTA TCGAAGCCCT
    GGAGAAGACC AGCTGTTCTC GGAGTTCTAC GTTGGCCACT TGGGATCAGG GATCAGGCTG
    CAAGTGAAAG ACAAGAAAGA TGAGACTCTG GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT
    CTCATGCCTG CAACTACTCT GATCCCTCCA GCCCGCATAG CCGTACCTTC ACCTCTGGAT
    GCCCCGCAGT TGCTGCACTT TGTGGACCAG TATCGAGAGC AGCTGATAGC CCGAGTGACA
    TCGGTGGAGG TTGTCTTGGA CAAACTGCAT GGACAGGTGC TGAGCCAGGA GCAGTACGAG
    AGGGTGCTGG CTGAGAACAC GAGGCCCAGC CAGATGCGGA AGCTGTTCAG CTTGAGCCAG
    TCCTGGGACC GGAAGTGCAA AGATGGACTC TACCAAGCCC TGAAGGAGAC CCATCCTCAC
    CTCATTATGG AACTCTGGGA GAAGGGCAGC AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25556
    Clone ID Related Accession (Same CDS sequence) NM_033007.4 , NM_033007.3
    Accession Version NM_033007.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229061.1, AB023143.1 and AI819334.1. On Dec 14, 2006 this sequence version replaced NM_033007.2. Transcript Variant: This variant (4) lacks two in-frame exons in the coding region, as compared to variant 1. The encoded isoform 4 is missing two internal segments, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229061.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGAACTGG AGCTCTGCTA TCGAAGCCCT
    GGAGAAGACC AGCTGTTCTC GGAGTTCTAC GTTGGCCACT TGGGATCAGG GATCAGGCTG
    CAAGTGAAAG ACAAGAAAGA TGAGACTCTG GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT
    CTCATGCCTG CAACTACTCT GATCCCTCCA GCCCGCATAG CCGTACCTTC ACCTCTGGAT
    GCCCCGCAGT TGCTGCACTT TGTGGACCAG TATCGAGAGC AGCTGATAGC CCGAGTGACA
    TCGGTGGAGG TTGTCTTGGA CAAACTGCAT GGACAGGTGC TGAGCCAGGA GCAGTACGAG
    AGGGTGCTGG CTGAGAACAC GAGGCCCAGC CAGATGCGGA AGCTGTTCAG CTTGAGCCAG
    TCCTGGGACC GGAAGTGCAA AGATGGACTC TACCAAGCCC TGAAGGAGAC CCATCCTCAC
    CTCATTATGG AACTCTGGGA GAAGGGCAGC AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127500.1
    CDS556..4755
    Translation

    Target ORF information:

    RefSeq Version NM_033007.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGAACTGG AGCTCTGCTA TCGAAGCCCT
    GGAGAAGACC AGCTGTTCTC GGAGTTCTAC GTTGGCCACT TGGGATCAGG GATCAGGCTG
    CAAGTGAAAG ACAAGAAAGA TGAGACTCTG GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT
    CTCATGCCTG CAACTACTCT GATCCCTCCA GCCCGCATAG CCGTACCTTC ACCTCTGGAT
    GCCCCGCAGT TGCTGCACTT TGTGGACCAG TATCGAGAGC AGCTGATAGC CCGAGTGACA
    TCGGTGGAGG TTGTCTTGGA CAAACTGCAT GGACAGGTGC TGAGCCAGGA GCAGTACGAG
    AGGGTGCTGG CTGAGAACAC GAGGCCCAGC CAGATGCGGA AGCTGTTCAG CTTGAGCCAG
    TCCTGGGACC GGAAGTGCAA AGATGGACTC TACCAAGCCC TGAAGGAGAC CCATCCTCAC
    CTCATTATGG AACTCTGGGA GAAGGGCAGC AAAAAGGGAC TCCTGCCACT CAGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25557
    Clone ID Related Accession (Same CDS sequence) NM_033006.3 , NM_033006.4
    Accession Version NM_033006.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229062.1, AB023143.1 and AI819334.1. On Dec 14, 2006 this sequence version replaced NM_033006.2. Transcript Variant: This variant (3) lacks an in-frame exon in the middle coding region, as compared to variant 1. The encoded isoform 3 is missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGCCATAG ATGATCTAGA AATGAAATTC
    CAGTTTGTGC GAATCCACAA GCCACCCCCG CTGACCCCAC TTTATATGGG CTGTCGTTAC
    ACTGTGTCTG GGTCTGGTTC AGGGATGCTG GAAATACTCC CCAAGGAACT GGAGCTCTGC
    TATCGAAGCC CTGGAGAAGA CCAGCTGTTC TCGGAGTTCT ACGTTGGCCA CTTGGGATCA
    GGGATCAGGC TGCAAGTGAA AGACAAGAAA GATGAGACTC TGGTGTGGGA GGCCTTGGTG
    AAACCAGGAG ATCTCATGCC TGCAACTACT CTGATCCCTC CAGCCCGCAT AGCCGTACCT
    TCACCTCTGG ATGCCCCGCA GTTGCTGCAC TTTGTGGACC AGTATCGAGA GCAGCTGATA
    GCCCGAGTGA CATCGGTGGA GGTTGTCTTG GACAAACTGC ATGGACAGGT GCTGAGCCAG
    GAGCAGTACG AGAGGGTGCT GGCTGAGAAC ACGAGGCCCA GCCAGATGCG GAAGCTGTTC
    AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC AAAGATGGAC TCTACCAAGC CCTGAAGGAG
    ACCCATCCTC ACCTCATTAT GGAACTCTGG GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA
    CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127499.1
    CDS556..4887
    Translation

    Target ORF information:

    RefSeq Version NM_033006.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033006.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGCCATAG ATGATCTAGA AATGAAATTC
    CAGTTTGTGC GAATCCACAA GCCACCCCCG CTGACCCCAC TTTATATGGG CTGTCGTTAC
    ACTGTGTCTG GGTCTGGTTC AGGGATGCTG GAAATACTCC CCAAGGAACT GGAGCTCTGC
    TATCGAAGCC CTGGAGAAGA CCAGCTGTTC TCGGAGTTCT ACGTTGGCCA CTTGGGATCA
    GGGATCAGGC TGCAAGTGAA AGACAAGAAA GATGAGACTC TGGTGTGGGA GGCCTTGGTG
    AAACCAGGAG ATCTCATGCC TGCAACTACT CTGATCCCTC CAGCCCGCAT AGCCGTACCT
    TCACCTCTGG ATGCCCCGCA GTTGCTGCAC TTTGTGGACC AGTATCGAGA GCAGCTGATA
    GCCCGAGTGA CATCGGTGGA GGTTGTCTTG GACAAACTGC ATGGACAGGT GCTGAGCCAG
    GAGCAGTACG AGAGGGTGCT GGCTGAGAAC ACGAGGCCCA GCCAGATGCG GAAGCTGTTC
    AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC AAAGATGGAC TCTACCAAGC CCTGAAGGAG
    ACCCATCCTC ACCTCATTAT GGAACTCTGG GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA
    CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25557
    Clone ID Related Accession (Same CDS sequence) NM_033006.3 , NM_033006.4
    Accession Version NM_033006.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055839.9, AF229062.1, AB023143.1 and AI819334.1. On May 31, 2019 this sequence version replaced NM_033006.3. Transcript Variant: This variant (3) lacks an in-frame exon in the middle coding region, as compared to variant 1. The encoded isoform 3 is missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGCCATAG ATGATCTAGA AATGAAATTC
    CAGTTTGTGC GAATCCACAA GCCACCCCCG CTGACCCCAC TTTATATGGG CTGTCGTTAC
    ACTGTGTCTG GGTCTGGTTC AGGGATGCTG GAAATACTCC CCAAGGAACT GGAGCTCTGC
    TATCGAAGCC CTGGAGAAGA CCAGCTGTTC TCGGAGTTCT ACGTTGGCCA CTTGGGATCA
    GGGATCAGGC TGCAAGTGAA AGACAAGAAA GATGAGACTC TGGTGTGGGA GGCCTTGGTG
    AAACCAGGAG ATCTCATGCC TGCAACTACT CTGATCCCTC CAGCCCGCAT AGCCGTACCT
    TCACCTCTGG ATGCCCCGCA GTTGCTGCAC TTTGTGGACC AGTATCGAGA GCAGCTGATA
    GCCCGAGTGA CATCGGTGGA GGTTGTCTTG GACAAACTGC ATGGACAGGT GCTGAGCCAG
    GAGCAGTACG AGAGGGTGCT GGCTGAGAAC ACGAGGCCCA GCCAGATGCG GAAGCTGTTC
    AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC AAAGATGGAC TCTACCAAGC CCTGAAGGAG
    ACCCATCCTC ACCTCATTAT GGAACTCTGG GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA
    CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_127499.1
    CDS553..4884
    Translation

    Target ORF information:

    RefSeq Version NM_033006.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family pyrin domain containing 1 (NLRP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033006.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGGCG GAGCCTGGGG CCGCCTGGCC TGTTACTTGG AGTTCCTGAA GAAGGAGGAG 
    CTGAAGGAGT TCCAGCTTCT GCTCGCCAAT AAAGCGCACT CCAGGAGCTC TTCGGGTGAG
    ACACCCGCTC AGCCAGAGAA GACGAGTGGC ATGGAGGTGG CCTCGTACCT GGTGGCTCAG
    TATGGGGAGC AGCGGGCCTG GGACCTAGCC CTCCATACCT GGGAGCAGAT GGGGCTGAGG
    TCACTGTGCG CCCAAGCCCA GGAAGGGGCA GGCCACTCTC CCTCATTCCC CTACAGCCCA
    AGTGAACCCC ACCTGGGGTC TCCCAGCCAA CCCACCTCCA CCGCAGTGCT AATGCCCTGG
    ATCCATGAAT TGCCGGCGGG GTGCACCCAG GGCTCAGAGA GAAGGGTTTT GAGACAGCTG
    CCTGACACAT CTGGACGCCG CTGGAGAGAA ATCTCTGCCT CACTCCTCTA CCAAGCTCTT
    CCAAGCTCCC CAGACCATGA GTCTCCAAGC CAGGAGTCAC CCAACGCCCC CACATCCACA
    GCAGTGCTGG GGAGCTGGGG ATCCCCACCT CAGCCCAGCC TAGCACCCAG AGAGCAGGAG
    GCTCCTGGGA CCCAATGGCC TCTGGATGAA ACGTCAGGAA TTTACTACAC AGAAATCAGA
    GAAAGAGAGA GAGAGAAATC AGAGAAAGGC AGGCCCCCAT GGGCAGCGGT GGTAGGAACG
    CCCCCACAGG CGCACACCAG CCTACAGCCC CACCACCACC CATGGGAGCC TTCTGTGAGA
    GAGAGCCTCT GTTCCACATG GCCCTGGAAA AATGAGGATT TTAACCAAAA ATTCACACAG
    CTGCTACTTC TACAAAGACC TCACCCCAGA AGCCAAGATC CCCTGGTCAA GAGAAGCTGG
    CCTGATTATG TGGAGGAGAA TCGAGGACAT TTAATTGAGA TCAGAGACTT ATTTGGCCCA
    GGCCTGGATA CCCAAGAACC TCGCATAGTC ATACTGCAGG GGGCTGCTGG AATTGGGAAG
    TCAACACTGG CCAGGCAGGT GAAGGAAGCC TGGGGGAGAG GCCAGCTGTA TGGGGACCGC
    TTCCAGCATG TCTTCTACTT CAGCTGCAGA GAGCTGGCCC AGTCCAAGGT GGTGAGTCTC
    GCTGAGCTCA TCGGAAAAGA TGGGACAGCC ACTCCGGCTC CCATTAGACA GATCCTGTCT
    AGGCCAGAGC GGCTGCTCTT CATCCTCGAT GGTGTAGATG AGCCAGGATG GGTCTTGCAG
    GAGCCGAGTT CTGAGCTCTG TCTGCACTGG AGCCAGCCAC AGCCGGCGGA TGCACTGCTG
    GGCAGTTTGC TGGGGAAAAC TATACTTCCC GAGGCATCCT TCCTGATCAC GGCTCGGACC
    ACAGCTCTGC AGAACCTCAT TCCTTCTTTG GAGCAGGCAC GTTGGGTAGA GGTCCTGGGG
    TTCTCTGAGT CCAGCAGGAA GGAATATTTC TACAGATATT TCACAGATGA AAGGCAAGCA
    ATTAGAGCCT TTAGGTTGGT CAAATCAAAC AAAGAGCTCT GGGCCCTGTG TCTTGTGCCC
    TGGGTGTCCT GGCTGGCCTG CACTTGCCTG ATGCAGCAGA TGAAGCGGAA GGAAAAACTC
    ACACTGACTT CCAAGACCAC CACAACCCTC TGTCTACATT ACCTTGCCCA GGCTCTCCAA
    GCTCAGCCAT TGGGACCCCA GCTCAGAGAC CTCTGCTCTC TGGCTGCTGA GGGCATCTGG
    CAAAAAAAGA CCCTTTTCAG TCCAGATGAC CTCAGGAAGC ATGGGTTAGA TGGGGCCATC
    ATCTCCACCT TCTTGAAGAT GGGTATTCTT CAAGAGCACC CCATCCCTCT GAGCTACAGC
    TTCATTCACC TCTGTTTCCA AGAGTTCTTT GCAGCAATGT CCTATGTCTT GGAGGATGAG
    AAGGGGAGAG GTAAACATTC TAATTGCATC ATAGATTTGG AAAAGACGCT AGAAGCATAT
    GGAATACATG GCCTGTTTGG GGCATCAACC ACACGTTTCC TATTGGGCCT GTTAAGTGAT
    GAGGGGGAGA GAGAGATGGA GAACATCTTT CACTGCCGGC TGTCTCAGGG GAGGAACCTG
    ATGCAGTGGG TCCCGTCCCT GCAGCTGCTG CTGCAGCCAC ACTCTCTGGA GTCCCTCCAC
    TGCTTGTACG AGACTCGGAA CAAAACGTTC CTGACACAAG TGATGGCCCA TTTCGAAGAA
    ATGGGCATGT GTGTAGAAAC AGACATGGAG CTCTTAGTGT GCACTTTCTG CATTAAATTC
    AGCCGCCACG TGAAGAAGCT TCAGCTGATT GAGGGCAGGC AGCACAGATC AACATGGAGC
    CCCACCATGG TAGTCCTGTT CAGGTGGGTC CCAGTCACAG ATGCCTATTG GCAGATTCTC
    TTCTCCGTCC TCAAGGTCAC CAGAAACCTG AAGGAGCTGG ACCTAAGTGG AAACTCGCTG
    AGCCACTCTG CAGTGAAGAG TCTTTGTAAG ACCCTGAGAC GCCCTCGCTG CCTCCTGGAG
    ACCCTGCGGT TGGCTGGCTG TGGCCTCACA GCTGAGGACT GCAAGGACCT TGCCTTTGGG
    CTGAGAGCCA ACCAGACCCT GACCGAGCTG GACCTGAGCT TCAATGTGCT CACGGATGCT
    GGAGCCAAAC ACCTTTGCCA GAGACTGAGA CAGCCGAGCT GCAAGCTACA GCGACTGCAG
    CTGGTCAGCT GTGGCCTCAC GTCTGACTGC TGCCAGGACC TGGCCTCTGT GCTTAGTGCC
    AGCCCCAGCC TGAAGGAGCT AGACCTGCAG CAGAACAACC TGGATGACGT TGGCGTGCGA
    CTGCTCTGTG AGGGGCTCAG GCATCCTGCC TGCAAACTCA TACGCCTGGG GAAACCAAGT
    GTGATGACCC CTACTGAGGG CCTGGATACG GGAGAGATGA GTAATAGCAC ATCCTCACTC
    AAGCGGCAGA GACTCGGATC AGAGAGGGCG GCTTCCCATG TTGCTCAGGC TAATCTCAAA
    CTCCTGGACG TGAGCAAGAT CTTCCCAATT GCTGAGATTG CAGAGGAAAG CTCCCCAGAG
    GTAGTACCGG TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG
    AAGCCTTTGG GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA
    GTTGACAAAG AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG
    CCCAACACGG GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT
    GTGTGGGACC AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT
    CTGCTGGACA TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG
    GCTCTCCAAG GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC
    AGCTTCTCCC CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC
    GTCACCTCTG TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC
    TACCTGATCC CAAGTGACTG CTCCATTCGG AAGGCCATAG ATGATCTAGA AATGAAATTC
    CAGTTTGTGC GAATCCACAA GCCACCCCCG CTGACCCCAC TTTATATGGG CTGTCGTTAC
    ACTGTGTCTG GGTCTGGTTC AGGGATGCTG GAAATACTCC CCAAGGAACT GGAGCTCTGC
    TATCGAAGCC CTGGAGAAGA CCAGCTGTTC TCGGAGTTCT ACGTTGGCCA CTTGGGATCA
    GGGATCAGGC TGCAAGTGAA AGACAAGAAA GATGAGACTC TGGTGTGGGA GGCCTTGGTG
    AAACCAGGAG ATCTCATGCC TGCAACTACT CTGATCCCTC CAGCCCGCAT AGCCGTACCT
    TCACCTCTGG ATGCCCCGCA GTTGCTGCAC TTTGTGGACC AGTATCGAGA GCAGCTGATA
    GCCCGAGTGA CATCGGTGGA GGTTGTCTTG GACAAACTGC ATGGACAGGT GCTGAGCCAG
    GAGCAGTACG AGAGGGTGCT GGCTGAGAAC ACGAGGCCCA GCCAGATGCG GAAGCTGTTC
    AGCTTGAGCC AGTCCTGGGA CCGGAAGTGC AAAGATGGAC TCTACCAAGC CCTGAAGGAG
    ACCCATCCTC ACCTCATTAT GGAACTCTGG GAGAAGGGCA GCAAAAAGGG ACTCCTGCCA
    CTCAGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.