FGA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FGA
Entrez Gene ID	2243
Full Name	fibrinogen alpha chain
Synonyms	Fib2
General protein information	Preferred Names fibrinogen alpha chain Names fibrinogen alpha chain fibrinogen, A alpha polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q31.3
Summary	This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. [provided by RefSeq, Jan 2016].
Disorder MIM:	134820
Disorder Html:	Dysfibrinogenemia, congenital, 616004 (3); Hypodysfibrinogenemia, congenital, 616004 (3); Amyloidosis, familial visceral, 105200 (3); Afibrinogenemia, congenital, 202400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021871.4	NP_068657.1	fibrinogen alpha chain isoform alpha precursor
NM_000508.5	NP_000499.1	fibrinogen alpha chain isoform alpha-E preproprotein
NM_000508.4	NP_000499.1	fibrinogen alpha chain isoform alpha-E preproprotein
NM_021871.3	NP_068657.1	fibrinogen alpha chain isoform alpha precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FGA	NP_000499.1
	FGA	XP_001088855.2
Canis lupus familiaris (dog)	FGA	XP_532697.2
Rattus norvegicus (Norway rat)	Fga	NP_001008724.1
Danio rerio (zebrafish)	fga	NP_001181918.1
Mus musculus (house mouse)	Fga	NP_001104518.1
Gallus gallus (chicken)	FGA	NP_001258840.1
Pan troglodytes (chimpanzee)	FGA	XP_001136067.1
Xenopus tropicalis (tropical clawed frog)	fga	XP_002933535.2

Gene Ontology

The following FGA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19142	NM_021871.4 Latest version!	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu19692	NM_000508.5 Latest version!	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$615.30 $879.00
View Old Accession Versions >>
OHu19692	NM_000508.4	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$615.30 $879.00
OHu19142	NM_021871.3	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FGA ( NM_021871.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068657.1 Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu19142
Clone ID Related Accession (Same CDS sequence)	NM_021871.4 , NM_021871.3
Accession Version	NM_021871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	fibrinogen alpha chain isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX494953.1, CB122305.1, CD608675.1, AL531808.3, AL579998.3, J00127.1 and J00128.1. On Nov 22, 2018 this sequence version replaced NM_021871.3. Transcript Variant: This variant (alpha) is alternatively spliced in the 3' coding region and 3' UTR, resulting in a shorter isoform (alpha) with a different carboxy-terminus compared to isoform alpha-E. The encoded isoform (alpha) may undergo proteolytic processing similar to isoform alpha-E. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.56643.1, SRR5189664.111793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000403106.8/ ENSP00000385981.3 RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GGTATCCACA CTTCTCCTTT GGGGAAGCCT 
TCCCTGTCCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068657.1
CDS	56..1990
Translation	MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRGIHTSPLGKPSLSP

Target ORF information:

RefSeq Version	NM_021871.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021871.4

ORF Insert Sequence:

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GGTATCCACA CTTCTCCTTT GGGGAAGCCT 
TCCCTGTCCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGA ( NM_021871.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068657.1 Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu19142
Clone ID Related Accession (Same CDS sequence)	NM_021871.4 , NM_021871.3
Accession Version	NM_021871.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	fibrinogen alpha chain isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN393696.1, AC107385.4, BX494953.1, CB122305.1, CD608675.1, AL531808.3, AL579998.3, J00127.1 and J00128.1. On Jan 8, 2016 this sequence version replaced NM_021871.2. Transcript Variant: This variant (alpha) is alternatively spliced in the 3' coding region and 3' UTR, resulting in a shorter isoform (alpha) with a different carboxy-terminus compared to isoform alpha-E. The encoded isoform (alpha) may undergo proteolytic processing similar to isoform alpha-E. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J00128.1, AK290559.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GGTATCCACA CTTCTCCTTT GGGGAAGCCT 
TCCCTGTCCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068657.1
CDS	80..2014
Translation	MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRGIHTSPLGKPSLSP

Target ORF information:

RefSeq Version	NM_021871.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021871.3

ORF Insert Sequence:

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GGTATCCACA CTTCTCCTTT GGGGAAGCCT 
TCCCTGTCCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGA ( NM_000508.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000499.1 Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.

Price & Availability↑

CloneID	OHu19692
Clone ID Related Accession (Same CDS sequence)	NM_000508.5 , NM_000508.4
Accession Version	NM_000508.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	fibrinogen alpha chain isoform alpha-E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX494953.1, BG565064.1, AC107385.4, BQ064506.1 and AF090936.1. On May 31, 2019 this sequence version replaced NM_000508.4. Transcript Variant: This variant (alpha-E) represents the longer transcript and encodes the longer isoform (alpha-E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GACTGTGATG ATGTCCTCCA AACACATCCT 
TCAGGTACCC AAAGTGGCAT TTTCAATATC AAGCTACCGG GATCCAGTAA GATTTTTTCT 
GTTTATTGCG ATCAAGAGAC CAGTTTGGGA GGATGGCTTT TGATCCAGCA AAGAATGGAT 
GGATCACTGA ATTTTAACCG GACCTGGCAA GACTACAAGA GAGGTTTCGG CAGCCTGAAT 
GACGAGGGGG AAGGAGAATT CTGGCTAGGC AATGACTACC TCCACTTACT AACCCAAAGG 
GGCTCTGTTC TTAGGGTTGA ATTAGAGGAC TGGGCTGGGA ATGAAGCTTA TGCAGAATAT 
CACTTCCGGG TAGGCTCTGA GGCTGAAGGC TATGCCCTCC AAGTCTCCTC CTATGAAGGC 
ACTGCGGGTG ATGCTCTGAT TGAGGGTTCC GTAGAGGAAG GGGCAGAGTA CACCTCTCAC 
AACAACATGC AGTTCAGCAC CTTTGACAGG GATGCAGACC AGTGGGAAGA GAACTGTGCA 
GAAGTCTATG GGGGAGGCTG GTGGTATAAT AACTGCCAAG CAGCCAATCT CAATGGAATC 
TACTACCCTG GGGGCTCCTA TGACCCAAGG AATAACAGTC CTTATGAGAT TGAGAATGGA 
GTGGTCTGGG TTTCCTTTAG AGGGGCAGAT TATTCCCTCA GGGCTGTTCG CATGAAAATT 
AGGCCCCTTG TGACCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000499.1
CDS	56..2656
Translation	MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRDCDDVLQTHPSGTQSGIFNIKLPGSSKIFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDEGEGEFWLGNDYLHLLTQRGSVLRVELEDWAGNEAYAEYHFRVGSEAEGYALQVSSYEGTAGDALIEGSVEEGAEYTSHNNMQFSTFDRDADQWEENCAEVYGGGWWYNNCQAANLNGIYYPGGSYDPRNNSPYEIENGVVWVSFRGADYSLRAVRMKIRPLVTQ

Target ORF information:

RefSeq Version	NM_000508.5
Organism	Homo sapiens(human)
Definition	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000508.5

ORF Insert Sequence:

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GACTGTGATG ATGTCCTCCA AACACATCCT 
TCAGGTACCC AAAGTGGCAT TTTCAATATC AAGCTACCGG GATCCAGTAA GATTTTTTCT 
GTTTATTGCG ATCAAGAGAC CAGTTTGGGA GGATGGCTTT TGATCCAGCA AAGAATGGAT 
GGATCACTGA ATTTTAACCG GACCTGGCAA GACTACAAGA GAGGTTTCGG CAGCCTGAAT 
GACGAGGGGG AAGGAGAATT CTGGCTAGGC AATGACTACC TCCACTTACT AACCCAAAGG 
GGCTCTGTTC TTAGGGTTGA ATTAGAGGAC TGGGCTGGGA ATGAAGCTTA TGCAGAATAT 
CACTTCCGGG TAGGCTCTGA GGCTGAAGGC TATGCCCTCC AAGTCTCCTC CTATGAAGGC 
ACTGCGGGTG ATGCTCTGAT TGAGGGTTCC GTAGAGGAAG GGGCAGAGTA CACCTCTCAC 
AACAACATGC AGTTCAGCAC CTTTGACAGG GATGCAGACC AGTGGGAAGA GAACTGTGCA 
GAAGTCTATG GGGGAGGCTG GTGGTATAAT AACTGCCAAG CAGCCAATCT CAATGGAATC 
TACTACCCTG GGGGCTCCTA TGACCCAAGG AATAACAGTC CTTATGAGAT TGAGAATGGA 
GTGGTCTGGG TTTCCTTTAG AGGGGCAGAT TATTCCCTCA GGGCTGTTCG CATGAAAATT 
AGGCCCCTTG TGACCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGA ( NM_000508.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000499.1 Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.

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CloneID	OHu19692
Clone ID Related Accession (Same CDS sequence)	NM_000508.5 , NM_000508.4
Accession Version	NM_000508.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	fibrinogen alpha chain isoform alpha-E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN393696.1, AC107385.4, BX494953.1, BG565064.1, BQ064506.1 and AF090936.1. On Jan 8, 2016 this sequence version replaced NM_000508.3. Transcript Variant: This variant (alpha-E) represents the longer transcript and encodes the longer isoform (alpha-E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GACTGTGATG ATGTCCTCCA AACACATCCT 
TCAGGTACCC AAAGTGGCAT TTTCAATATC AAGCTACCGG GATCCAGTAA GATTTTTTCT 
GTTTATTGCG ATCAAGAGAC CAGTTTGGGA GGATGGCTTT TGATCCAGCA AAGAATGGAT 
GGATCACTGA ATTTTAACCG GACCTGGCAA GACTACAAGA GAGGTTTCGG CAGCCTGAAT 
GACGAGGGGG AAGGAGAATT CTGGCTAGGC AATGACTACC TCCACTTACT AACCCAAAGG 
GGCTCTGTTC TTAGGGTTGA ATTAGAGGAC TGGGCTGGGA ATGAAGCTTA TGCAGAATAT 
CACTTCCGGG TAGGCTCTGA GGCTGAAGGC TATGCCCTCC AAGTCTCCTC CTATGAAGGC 
ACTGCGGGTG ATGCTCTGAT TGAGGGTTCC GTAGAGGAAG GGGCAGAGTA CACCTCTCAC 
AACAACATGC AGTTCAGCAC CTTTGACAGG GATGCAGACC AGTGGGAAGA GAACTGTGCA 
GAAGTCTATG GGGGAGGCTG GTGGTATAAT AACTGCCAAG CAGCCAATCT CAATGGAATC 
TACTACCCTG GGGGCTCCTA TGACCCAAGG AATAACAGTC CTTATGAGAT TGAGAATGGA 
GTGGTCTGGG TTTCCTTTAG AGGGGCAGAT TATTCCCTCA GGGCTGTTCG CATGAAAATT 
AGGCCCCTTG TGACCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000499.1
CDS	80..2680
Translation	MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRDCDDVLQTHPSGTQSGIFNIKLPGSSKIFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDEGEGEFWLGNDYLHLLTQRGSVLRVELEDWAGNEAYAEYHFRVGSEAEGYALQVSSYEGTAGDALIEGSVEEGAEYTSHNNMQFSTFDRDADQWEENCAEVYGGGWWYNNCQAANLNGIYYPGGSYDPRNNSPYEIENGVVWVSFRGADYSLRAVRMKIRPLVTQ

Target ORF information:

RefSeq Version	NM_000508.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fibrinogen alpha chain (FGA), transcript variant alpha-E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000508.4

ORF Insert Sequence:

ATGTTTTCCA TGAGGATCGT CTGCCTGGTC CTAAGTGTGG TGGGCACAGC ATGGACTGCA 
GATAGTGGTG AAGGTGACTT TCTAGCTGAA GGAGGAGGCG TGCGTGGCCC AAGGGTTGTG 
GAAAGACATC AATCTGCCTG CAAAGATTCA GACTGGCCCT TCTGCTCTGA TGAAGACTGG 
AACTACAAAT GCCCTTCTGG CTGCAGGATG AAAGGGTTGA TTGATGAAGT CAATCAAGAT 
TTTACAAACA GAATAAATAA GCTCAAAAAT TCACTATTTG AATATCAGAA GAACAATAAG 
GATTCTCATT CGTTGACCAC TAATATAATG GAAATTTTGA GAGGCGATTT TTCCTCAGCC 
AATAACCGTG ATAATACCTA CAACCGAGTG TCAGAGGATC TGAGAAGCAG AATTGAAGTC 
CTGAAGCGCA AAGTCATAGA AAAAGTACAG CATATCCAGC TTCTGCAGAA AAATGTTAGA 
GCTCAGTTGG TTGATATGAA ACGACTGGAG GTGGACATTG ATATTAAGAT CCGATCTTGT 
CGAGGGTCAT GCAGTAGGGC TTTAGCTCGT GAAGTAGATC TGAAGGACTA TGAAGATCAG 
CAGAAGCAAC TTGAACAGGT CATTGCCAAA GACTTACTTC CCTCTAGAGA TAGGCAACAC 
TTACCACTGA TAAAAATGAA ACCAGTTCCA GACTTGGTTC CCGGAAATTT TAAGAGCCAG 
CTTCAGAAGG TACCCCCAGA GTGGAAGGCA TTAACAGACA TGCCGCAGAT GAGAATGGAG 
TTAGAGAGAC CTGGTGGAAA TGAGATTACT CGAGGAGGCT CCACCTCTTA TGGAACCGGA 
TCAGAGACGG AAAGCCCCAG GAACCCTAGC AGTGCTGGAA GCTGGAACTC TGGGAGCTCT 
GGACCTGGAA GTACTGGAAA CCGAAACCCT GGGAGCTCTG GGACTGGAGG GACTGCAACC 
TGGAAACCTG GGAGCTCTGG ACCTGGAAGT ACTGGAAGCT GGAACTCTGG GAGCTCTGGA 
ACTGGAAGTA CTGGAAACCA AAACCCTGGG AGCCCTAGAC CTGGTAGTAC CGGAACCTGG 
AATCCTGGCA GCTCTGAACG CGGAAGTGCT GGGCACTGGA CCTCTGAGAG CTCTGTATCT 
GGTAGTACTG GACAATGGCA CTCTGAATCT GGAAGTTTTA GGCCAGATAG CCCAGGCTCT 
GGGAACGCGA GGCCTAACAA CCCAGACTGG GGCACATTTG AAGAGGTGTC AGGAAATGTA 
AGTCCAGGGA CAAGGAGAGA GTACCACACA GAAAAACTGG TCACTTCTAA AGGAGATAAA 
GAGCTCAGGA CTGGTAAAGA GAAGGTCACC TCTGGTAGCA CAACCACCAC GCGTCGTTCA 
TGCTCTAAAA CCGTTACTAA GACTGTTATT GGTCCTGATG GTCACAAAGA AGTTACCAAA 
GAAGTGGTGA CCTCCGAAGA TGGTTCTGAC TGTCCCGAGG CAATGGATTT AGGCACATTG 
TCTGGCATAG GTACTCTGGA TGGGTTCCGC CATAGGCACC CTGATGAAGC TGCCTTCTTC 
GACACTGCCT CAACTGGAAA AACATTCCCA GGTTTCTTCT CACCTATGTT AGGAGAGTTT 
GTCAGTGAGA CTGAGTCTAG GGGCTCAGAA TCTGGCATCT TCACAAATAC AAAGGAATCC 
AGTTCTCATC ACCCTGGGAT AGCTGAATTC CCTTCCCGTG GTAAATCTTC AAGTTACAGC 
AAACAATTTA CTAGTAGCAC GAGTTACAAC AGAGGAGACT CCACATTTGA AAGCAAGAGC 
TATAAAATGG CAGATGAGGC CGGAAGTGAA GCCGATCATG AAGGAACACA TAGCACCAAG 
AGAGGCCATG CTAAATCTCG CCCTGTCAGA GACTGTGATG ATGTCCTCCA AACACATCCT 
TCAGGTACCC AAAGTGGCAT TTTCAATATC AAGCTACCGG GATCCAGTAA GATTTTTTCT 
GTTTATTGCG ATCAAGAGAC CAGTTTGGGA GGATGGCTTT TGATCCAGCA AAGAATGGAT 
GGATCACTGA ATTTTAACCG GACCTGGCAA GACTACAAGA GAGGTTTCGG CAGCCTGAAT 
GACGAGGGGG AAGGAGAATT CTGGCTAGGC AATGACTACC TCCACTTACT AACCCAAAGG 
GGCTCTGTTC TTAGGGTTGA ATTAGAGGAC TGGGCTGGGA ATGAAGCTTA TGCAGAATAT 
CACTTCCGGG TAGGCTCTGA GGCTGAAGGC TATGCCCTCC AAGTCTCCTC CTATGAAGGC 
ACTGCGGGTG ATGCTCTGAT TGAGGGTTCC GTAGAGGAAG GGGCAGAGTA CACCTCTCAC 
AACAACATGC AGTTCAGCAC CTTTGACAGG GATGCAGACC AGTGGGAAGA GAACTGTGCA 
GAAGTCTATG GGGGAGGCTG GTGGTATAAT AACTGCCAAG CAGCCAATCT CAATGGAATC 
TACTACCCTG GGGGCTCCTA TGACCCAAGG AATAACAGTC CTTATGAGAT TGAGAATGGA 
GTGGTCTGGG TTTCCTTTAG AGGGGCAGAT TATTCCCTCA GGGCTGTTCG CATGAAAATT 
AGGCCCCTTG TGACCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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FGA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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