THSD7A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	THSD7A
Entrez Gene ID	221981
Full Name	thrombospondin type 1 domain containing 7A
General protein information	Preferred Names thrombospondin type 1 domain containing 7A Names thrombospondin type-1 domain-containing protein 7A thrombospondin, type I, domain containing 7A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p21.3
Summary	The protein encoded by this gene is found almost exclusively in endothelial cells from placenta and umbilical cord. The encoded protein appears to interact with alpha(V)beta(3) integrin and paxillin to inhibit endothelial cell migration and tube formation. This protein may be involved in cytoskeletal organization. Variations in this gene may be associated with low bone mineral density in osteoporosis. [provided by RefSeq, Aug 2010].
Disorder MIM:	612249

mRNA and Protein(s)

mRNA	Protein	Name
XM_011515193.2	XP_011513495.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_011515194.1	XP_011513496.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_006715660.1	XP_006715723.1	thrombospondin type-1 domain-containing protein 7A isoform X3
NM_015204.2	NP_056019.1	thrombospondin type-1 domain-containing protein 7A precursor
NM_015204.3	NP_056019.1	thrombospondin type-1 domain-containing protein 7A precursor
XM_006715659.1	XP_006715722.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_011515193.3	XP_011513495.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_006715662.1	XP_006715725.1	thrombospondin type-1 domain-containing protein 7A isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	THSD7A	NP_056019.1
Canis lupus familiaris (dog)	THSD7A	XP_003431913.2
Bos taurus (cattle)	THSD7A	NP_001193672.1
Danio rerio (zebrafish)	thsd7a	NP_001116232.1
Mus musculus (house mouse)	Thsd7a	NP_001158277.1
Pan troglodytes (chimpanzee)	THSD7A	XP_527669.2
Rattus norvegicus (Norway rat)	Thsd7a	NP_001178899.1
Gallus gallus (chicken)	THSD7A	XP_418686.3
Xenopus tropicalis (tropical clawed frog)	thsd7a	XP_002939058.2

Gene Ontology
Bibliography

Related articles in PubMed

Circulating Antibodies against Thrombospondin Type-I Domain-Containing 7A in Chinese Patients with Idiopathic Membranous Nephropathy.
Wang J, Cui Z, Lu J, Probst C, Zhang YM, Wang X, Qu Z, Wang F, Meng LQ, Cheng XY, Liu G, Debiec H, Ronco P, Zhao MH
Clinical journal of the American Society of Nephrology : CJASN12(10)1642-1651(2017 Oct)

Recent Progress in Deciphering the Etiopathogenesis of Primary Membranous Nephropathy.
Kronbichler A, Oh J, Meijers B, Mayer G, Shin JI
BioMed research international20171936372(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High THSD7A expression is associated with membranous nephropathy and cancer.
Title: THSD7A expression in human cancer.

This study provides novel data on the function of THSD7A in human placental cells, and extends knowledge of how miR-210 is involved in the development of the preeclampsia.
Title: Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain containing 7A.

THSD7A is neural N-glycoprotein, which promotes angiogenesis and it is well known that angiogenesis modulates obesity, adipose metabolism and insulin sensitivity, hence our result find a correlation.
Title: A novel gene THSD7A is associated with obesity.

Data indicate that enhanced granular expression of phospholipase A2 receptor (PLA2R) and thrombospondin type-1 domain-containing 7A (THSD7A) was detected in 9.1% and 52.7%, respectively, of the patients with idiopathic membranous nephropathy (MN).
Title: Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy.

15 of 154 patients with idiopathic membranous nephropathy had circulating autoantibodies to THSD7A but not to PLA2R1, a finding that suggests a distinct subgroup of patients with this condition.
Title: Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy.

The following THSD7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THSD7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22546	NM_015204.3 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu78075	XM_011515194.1 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu78075	XM_011515193.3 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu41099	XM_006715659.1 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41100	XM_006715660.1 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41102	XM_006715662.1 Latest version!	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu22546	NM_015204.2	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu78075	XM_011515193.2	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THSD7A ( NM_015204.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056019.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.

Price & Availability↑

CloneID	OHu22546
Clone ID Related Accession (Same CDS sequence)	NM_015204.2 , NM_015204.3
Accession Version	NM_015204.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092252.1, AC004614.2, AB023177.2, AA297188.1, BF111945.1, AK001884.1, AC004160.1 and BC030083.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2009 this sequence version replaced NM_015204.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GATGGGAGAC TAAAGACCTG GGTTTACGGT GTAGCAGCTG GGGCATTTGT GTTACTCATC 
TTTATTGTCT CCATGATTTA TCTAGCTTGC AAAAAGCCAA AGAAACCCCA AAGAAGGCAA 
AACAACCGAC TGAAACCTTT AACCTTAGCC TATGATGGAG ATGCCGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056019.1
CDS	253..5226
Translation	MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	NM_015204.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015204.2

ORF Insert Sequence:

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GATGGGAGAC TAAAGACCTG GGTTTACGGT GTAGCAGCTG GGGCATTTGT GTTACTCATC 
TTTATTGTCT CCATGATTTA TCTAGCTTGC AAAAAGCCAA AGAAACCCCA AAGAAGGCAA 
AACAACCGAC TGAAACCTTT AACCTTAGCC TATGATGGAG ATGCCGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( NM_015204.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056019.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.

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CloneID	OHu22546
Clone ID Related Accession (Same CDS sequence)	NM_015204.2 , NM_015204.3
Accession Version	NM_015204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092252.1, AC004614.2, AB023177.2, AA297188.1, BF111945.1, AK001884.1, AC004160.1 and BC030083.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_015204.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000423059.9/ ENSP00000406482.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GATGGGAGAC TAAAGACCTG GGTTTACGGT GTAGCAGCTG GGGCATTTGT GTTACTCATC 
TTTATTGTCT CCATGATTTA TCTAGCTTGC AAAAAGCCAA AGAAACCCCA AAGAAGGCAA 
AACAACCGAC TGAAACCTTT AACCTTAGCC TATGATGGAG ATGCCGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056019.1
CDS	253..5226
Translation	MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	NM_015204.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015204.3

ORF Insert Sequence:

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GATGGGAGAC TAAAGACCTG GGTTTACGGT GTAGCAGCTG GGGCATTTGT GTTACTCATC 
TTTATTGTCT CCATGATTTA TCTAGCTTGC AAAAAGCCAA AGAAACCCCA AAGAAGGCAA 
AACAACCGAC TGAAACCTTT AACCTTAGCC TATGATGGAG ATGCCGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_011515193.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513495.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu78075
Clone ID Related Accession (Same CDS sequence)	XM_011515193.2 , XM_011515194.1 , XM_011515193.3
Accession Version	XM_011515193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513495.1
CDS	2784..7550
Translation	MGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_011515193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515193.2

ORF Insert Sequence:

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_011515194.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513496.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X3, mRNA.

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CloneID	OHu78075
Clone ID Related Accession (Same CDS sequence)	XM_011515193.2 , XM_011515194.1 , XM_011515193.3
Accession Version	XM_011515194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513496.1
CDS	225..4991
Translation	MGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_011515194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515194.1

ORF Insert Sequence:

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_011515193.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513495.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.

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CloneID	OHu78075
Clone ID Related Accession (Same CDS sequence)	XM_011515193.2 , XM_011515194.1 , XM_011515193.3
Accession Version	XM_011515193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513495.1
CDS	2785..7551
Translation	MGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_011515193.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515193.3

ORF Insert Sequence:

ATGGGAGATG AATGTGGTCC CGGAGGCATC CAAACGAGGG CTGTGTGGTG TGCTCATGTG 
GAGGGATGGA CTACACTGCA TACTAACTGT AAGCAGGCCG AGAGACCCAA TAACCAGCAG 
AATTGTTTCA AAGTTTGCGA TTGGCACAAA GAGTTGTACG ACTGGAGACT GGGACCTTGG 
AATCAGTGTC AGCCCGTGAT TTCAAAAAGC CTAGAGAAAC CTCTTGAGTG CATTAAGGGG 
GAAGAAGGTA TTCAGGTGAG GGAGATAGCG TGCATCCAGA AAGACAAAGA CATTCCTGCG 
GAGGATATCA TCTGTGAGTA CTTTGAGCCC AAGCCTCTCC TGGAGCAGGC TTGCCTCATT 
CCTTGCCAGC AAGATTGCAT CGTGTCTGAA TTTTCTGCCT GGTCCGAATG CTCCAAGACC 
TGCGGCAGCG GGCTCCAGCA CCGGACGCGT CATGTGGTGG CGCCCCCGCA GTTCGGAGGC 
TCTGGCTGTC CAAACCTGAC GGAGTTCCAG GTGTGCCAAT CCAGTCCATG CGAGGCCGAG 
GAGCTCAGGT ACAGCCTGCA TGTGGGGCCC TGGAGCACCT GCTCAATGCC CCACTCCCGA 
CAAGTAAGAC AAGCAAGGAG ACGCGGGAAG AATAAAGAAC GGGAAAAGGA CCGCAGCAAA 
GGAGTAAAGG ATCCAGAAGC CCGCGAGCTT ATTAAGAAAA AGAGAAACAG AAACAGGCAG 
AACAGACAAG AGAACAAATA TTGGGACATC CAGATTGGAT ATCAGACCAG AGAGGTTATG 
TGCATTAACA AGACGGGGAA AGCTGCTGAT TTAAGCTTTT GCCAGCAAGA GAAGCTTCCA 
ATGACCTTCC AGTCCTGTGT GATCACCAAA GAGTGCCAGG TTTCCGAGTG GTCAGAGTGG 
AGCCCCTGCT CAAAAACATG CCATGACATG GTGTCCCCTG CAGGCACTCG TGTAAGGACA 
CGAACCATCA GGCAGTTTCC CATTGGCAGT GAAAAGGAGT GTCCAGAATT TGAAGAAAAA 
GAACCCTGTT TGTCTCAAGG AGATGGAGTT GTCCCCTGTG CCACGTATGG CTGGAGAACT 
ACAGAGTGGA CTGAGTGCCG TGTGGACCCT TTGCTCAGTC AGCAGGACAA GAGGCGCGGC 
AACCAGACGG CCCTCTGTGG AGGGGGCATC CAGACCCGAG AGGTGTACTG CGTGCAGGCC 
AACGAAAACC TCCTCTCACA ATTAAGTACC CACAAGAACA AAGAAGCCTC AAAGCCAATG 
GACTTAAAAT TATGCACTGG ACCTATCCCT AATACTACAC AGCTGTGCCA CATTCCTTGT 
CCAACTGAAT GTGAAGTTTC ACCTTGGTCA GCTTGGGGAC CTTGTACTTA TGAAAACTGT 
AATGATCAGC AAGGGAAAAA AGGCTTCAAA CTGAGGAAGC GGCGCATTAC CAATGAGCCC 
ACTGGAGGCT CTGGGGTAAC CGGAAACTGC CCTCACTTAC TGGAAGCCAT TCCCTGTGAA 
GAGCCTGCCT GTTATGACTG GAAAGCAGTG AGACTGGGAA ACTGCGAGCC AGATAACGGA 
AAGGAGTGTG GTCCAGGCAC GCAAGTTCAA GAGGTTGTGT GCATCAACAG TGATGGAGAA 
GAAGTTGACA GACAGCTGTG CAGAGATGCC ATCTTCCCCA TCCCTGTGGC CTGTGATGCC 
CCATGCCCGA AAGACTGTGT GCTCAGCACA TGGTCTACGT GGTCCTCCTG CTCACACACC 
TGCTCAGGGA AAACGACAGA AGGGAAACAG ATACGAGCAC GATCCATTCT GGCCTATGCG 
GGTGAAGAAG GTGGAATTCG CTGTCCAAAT AGCAGTGCTT TGCAAGAAGT ACGAAGCTGT 
AATGAGCATC CTTGCACAGT GTACCACTGG CAAACTGGTC CCTGGGGCCA GTGCATTGAG 
GACACCTCAG TATCGTCCTT CAACACAACT ACGACTTGGA ATGGGGAGGC CTCCTGCTCT 
GTCGGCATGC AGACAAGAAA AGTCATCTGT GTGCGAGTCA ATGTGGGCCA AGTGGGACCC 
AAAAAATGTC CTGAAAGCCT TCGACCTGAA ACTGTAAGGC CTTGTCTGCT TCCTTGTAAG 
AAGGACTGTA TTGTGACCCC ATATAGTGAC TGGACATCAT GCCCCTCTTC GTGTAAAGAA 
GGGGACTCCA GTATCAGGAA GCAGTCTAGG CATCGGGTCA TCATTCAGCT GCCAGCCAAC 
GGGGGCCGAG ACTGCACAGA TCCCCTCTAT GAAGAGAAGG CCTGTGAGGC ACCTCAAGCG 
TGCCAAAGCT ACAGGTGGAA GACTCACAAA TGGCGCAGAT GCCAATTAGT CCCTTGGAGC 
GTGCAACAAG ACAGCCCTGG AGCACAGGAA GGCTGTGGGC CTGGGCGACA GGCAAGAGCC 
ATTACTTGTC GCAAGCAAGA TGGAGGACAG GCTGGAATCC ATGAGTGCCT ACAGTATGCA 
GGCCCTGTGC CAGCCCTTAC CCAGGCCTGC CAGATCCCCT GCCAGGATGA CTGTCAATTG 
ACCAGCTGGT CCAAGTTTTC TTCATGCAAT GGAGACTGTG GTGCAGTTAG GACCAGAAAG 
CGCACTCTTG TTGGAAAAAG TAAAAAGAAG GAAAAATGTA AAAATTCCCA TTTGTATCCC 
CTGATTGAGA CTCAGTATTG TCCTTGTGAC AAATATAATG CACAACCTGT GGGGAACTGG 
TCAGACTGTA TTTTACCAGA GGGAAAAGTG GAAGTGTTGC TGGGAATGAA AGTACAAGGA 
GACATCAAGG AATGCGGACA AGGATATCGT TACCAAGCAA TGGCATGCTA CGATCAAAAT 
GGCAGGCTTG TGGAAACATC TAGATGTAAC AGCCATGGTT ACATTGAGGA GGCCTGCATC 
ATCCCCTGCC CCTCAGACTG CAAGCTCAGT GAGTGGTCCA ACTGGTCGCG CTGCAGCAAG 
TCCTGTGGGA GTGGTGTGAA GGTTCGTTCT AAATGGCTGC GTGAAAAACC ATATAATGGA 
GGAAGGCCTT GCCCCAAACT GGACCATGTC AACCAGGCAC AGGTGTATGA GGTTGTCCCA 
TGCCACAGTG ACTGCAACCA GTACCTATGG GTCACAGAGC CCTGGAGCAT CTGCAAGGTG 
ACCTTTGTGA ATATGCGGGA GAACTGTGGA GAGGGCGTGC AAACCCGAAA AGTGAGATGC 
ATGCAGAATA CAGCAGATGG CCCTTCTGAA CATGTAGAGG ATTACCTCTG TGACCCAGAA 
GAGATGCCCC TGGGCTCTAG AGTGTGCAAA TTACCATGCC CTGAGGACTG TGTGATATCT 
GAATGGGGTC CATGGACCCA ATGTGTTTTG CCTTGCAATC AAAGCAGTTT CCGGCAAAGG 
TCAGCTGATC CCATCAGACA ACCAGCTGAT GAAGGAAGAT CTTGCCCTAA TGCTGTTGAG 
AAAGAACCCT GTAACCTGAA CAAAAACTGC TACCACTATG ATTATAATGT AACAGACTGG 
AGTACATGTC AGCTGAGTGA GAAGGCAGTT TGTGGAAATG GAATAAAAAC AAGGATGTTG 
GATTGTGTTC GAAGTGATGG CAAGTCAGTT GACCTGAAAT ATTGTGAAGC GCTTGGCTTG 
GAGAAGAACT GGCAGATGAA CACGTCCTGC ATGGTGGAAT GCCCTGTGAA CTGTCAGCTT 
TCTGATTGGT CTCCTTGGTC AGAATGTTCT CAAACATGTG GCCTCACAGG AAAAATGATC 
CGAAGACGAA CAGTGACCCA GCCCTTTCAA GGTGATGGAA GACCATGCCC TTCCCTGATG 
GACCAGTCCA AACCCTGCCC AGTGAAGCCT TGTTATCGGT GGCAATATGG CCAGTGGTCT 
CCATGCCAAG TGCAGGAGGC CCAGTGTGGA GAAGGGACCA GAACAAGGAA CATTTCTTGT 
GTAGTAAGTG ATGGGTCAGC TGATGATTTC AGCAAAGTGG TGGATGAGGA ATTCTGTGCT 
GACATTGAAC TCATTATAGA TGGTAATAAA AATATGGTTC TGGAGGAATC CTGCAGCCAG 
CCTTGCCCAG GTGACTGTTA TTTGAAGGAC TGGTCTTCCT GGAGCCTGTG TCAGCTGACC 
TGTGTGAATG GTGAGGATCT AGGCTTTGGT GGAATACAGG TCAGATCCAG ACCGGTGATT 
ATACAAGAAC TAGAGAATCA GCATCTGTGC CCAGAGCAGA TGTTAGAAAC AAAATCATGT 
TATGATGGAC AGTGCTATGA ATATAAATGG ATGGCCAGTG CTTGGAAGGG CTCTTCCCGA 
ACAGTGTGGT GTCAAAGGTC AGATGGTATA AATGTAACAG GGGGCTGCTT GGTGATGAGC 
CAGCCTGATG CCGACAGGTC TTGTAACCCA CCGTGTAGTC AACCCCACTC GTACTGTAGC 
GAGACAAAAA CATGCCATTG TGAAGAAGGG TACACTGAAG TCATGTCTTC TAACAGCACC 
CTTGAGCAAT GCACACTTAT CCCCGTGGTG GTATTACCCA CCATGGAGGA CAAAAGAGGA 
GATGTGAAAA CCAGTCGGGC TGTACATCCA ACCCAACCCT CCAGTAACCC AGCAGGACGG 
GGAAGGACCT GGTTTCTACA GCCATTTGGG CCAGATGGGA GACTAAAGAC CTGGGTTTAC 
GGTGTAGCAG CTGGGGCATT TGTGTTACTC ATCTTTATTG TCTCCATGAT TTATCTAGCT 
TGCAAAAAGC CAAAGAAACC CCAAAGAAGG CAAAACAACC GACTGAAACC TTTAACCTTA 
GCCTATGATG GAGATGCCGA CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_006715659.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715722.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X1, mRNA.

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CloneID	OHu41099
Clone ID Related Accession (Same CDS sequence)	XM_006715659.1
Accession Version	XM_006715659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GCAAAAAGCC AAAGAAACCC CAAAGAAGGC AAAACAACCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715722.1
CDS	252..5096
Translation	MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPAKSQRNPKEGKTTD

Target ORF information:

RefSeq Version	XM_006715659.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715659.1

ORF Insert Sequence:

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGGGG GCTGCTTGGT GATGAGCCAG CCTGATGCCG ACAGGTCTTG TAACCCACCG 
TGTAGTCAAC CCCACTCGTA CTGTAGCGAG ACAAAAACAT GCCATTGTGA AGAAGGGTAC 
ACTGAAGTCA TGTCTTCTAA CAGCACCCTT GAGCAATGCA CACTTATCCC CGTGGTGGTA 
TTACCCACCA TGGAGGACAA AAGAGGAGAT GTGAAAACCA GTCGGGCTGT ACATCCAACC 
CAACCCTCCA GTAACCCAGC AGGACGGGGA AGGACCTGGT TTCTACAGCC ATTTGGGCCA 
GCAAAAAGCC AAAGAAACCC CAAAGAAGGC AAAACAACCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_006715660.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715723.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X4, mRNA.

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CloneID	OHu41100
Clone ID Related Accession (Same CDS sequence)	XM_006715660.1
Accession Version	XM_006715660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGATG GGAGACTAAA GACCTGGGTT TACGGTGTAG CAGCTGGGGC ATTTGTGTTA 
CTCATCTTTA TTGTCTCCAT GATTTATCTA GCTTGCAAAA AGCCAAAGAA ACCCCAAAGA 
AGGCAAAACA ACCGACTGAA ACCTTTAACC TTAGCCTATG ATGGAGATGC CGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715723.1
CDS	252..4931
Translation	MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTDGRLKTWVYGVAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_006715660.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715660.1

ORF Insert Sequence:

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGATG GGAGACTAAA GACCTGGGTT TACGGTGTAG CAGCTGGGGC ATTTGTGTTA 
CTCATCTTTA TTGTCTCCAT GATTTATCTA GCTTGCAAAA AGCCAAAGAA ACCCCAAAGA 
AGGCAAAACA ACCGACTGAA ACCTTTAACC TTAGCCTATG ATGGAGATGC CGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD7A ( XM_006715662.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715725.1 Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X6, mRNA.

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CloneID	OHu41102
Clone ID Related Accession (Same CDS sequence)	XM_006715662.1
Accession Version	XM_006715662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 7A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGCAA AAAGCCAAAG AAACCCCAAA GAAGGCAAAA CAACCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715725.1
CDS	252..4802
Translation	MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGINVTAKSQRNPKEGKTTD

Target ORF information:

RefSeq Version	XM_006715662.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715662.1

ORF Insert Sequence:

ATGGGGCTGC AAGCCAGGCG CTGGGCGTCC GGGAGCCGGG GCGCTGCGGG GCCGCGCCGG 
GGCGTCCTGC AGCTGCTGCC GCTGCCGCTG CCGCTGCCGC TGCTCCTGCT GCTGCTGCTA 
CGCCCGGGCG CCGGCAGGGC TGCGGCGCAG GGCGAGGCGG AGGCGCCCAC CCTCTATCTG 
TGGAAGACTG GTCCATGGGG CCGATGTATG GGAGATGAAT GTGGTCCCGG AGGCATCCAA 
ACGAGGGCTG TGTGGTGTGC TCATGTGGAG GGATGGACTA CACTGCATAC TAACTGTAAG 
CAGGCCGAGA GACCCAATAA CCAGCAGAAT TGTTTCAAAG TTTGCGATTG GCACAAAGAG 
TTGTACGACT GGAGACTGGG ACCTTGGAAT CAGTGTCAGC CCGTGATTTC AAAAAGCCTA 
GAGAAACCTC TTGAGTGCAT TAAGGGGGAA GAAGGTATTC AGGTGAGGGA GATAGCGTGC 
ATCCAGAAAG ACAAAGACAT TCCTGCGGAG GATATCATCT GTGAGTACTT TGAGCCCAAG 
CCTCTCCTGG AGCAGGCTTG CCTCATTCCT TGCCAGCAAG ATTGCATCGT GTCTGAATTT 
TCTGCCTGGT CCGAATGCTC CAAGACCTGC GGCAGCGGGC TCCAGCACCG GACGCGTCAT 
GTGGTGGCGC CCCCGCAGTT CGGAGGCTCT GGCTGTCCAA ACCTGACGGA GTTCCAGGTG 
TGCCAATCCA GTCCATGCGA GGCCGAGGAG CTCAGGTACA GCCTGCATGT GGGGCCCTGG 
AGCACCTGCT CAATGCCCCA CTCCCGACAA GTAAGACAAG CAAGGAGACG CGGGAAGAAT 
AAAGAACGGG AAAAGGACCG CAGCAAAGGA GTAAAGGATC CAGAAGCCCG CGAGCTTATT 
AAGAAAAAGA GAAACAGAAA CAGGCAGAAC AGACAAGAGA ACAAATATTG GGACATCCAG 
ATTGGATATC AGACCAGAGA GGTTATGTGC ATTAACAAGA CGGGGAAAGC TGCTGATTTA 
AGCTTTTGCC AGCAAGAGAA GCTTCCAATG ACCTTCCAGT CCTGTGTGAT CACCAAAGAG 
TGCCAGGTTT CCGAGTGGTC AGAGTGGAGC CCCTGCTCAA AAACATGCCA TGACATGGTG 
TCCCCTGCAG GCACTCGTGT AAGGACACGA ACCATCAGGC AGTTTCCCAT TGGCAGTGAA 
AAGGAGTGTC CAGAATTTGA AGAAAAAGAA CCCTGTTTGT CTCAAGGAGA TGGAGTTGTC 
CCCTGTGCCA CGTATGGCTG GAGAACTACA GAGTGGACTG AGTGCCGTGT GGACCCTTTG 
CTCAGTCAGC AGGACAAGAG GCGCGGCAAC CAGACGGCCC TCTGTGGAGG GGGCATCCAG 
ACCCGAGAGG TGTACTGCGT GCAGGCCAAC GAAAACCTCC TCTCACAATT AAGTACCCAC 
AAGAACAAAG AAGCCTCAAA GCCAATGGAC TTAAAATTAT GCACTGGACC TATCCCTAAT 
ACTACACAGC TGTGCCACAT TCCTTGTCCA ACTGAATGTG AAGTTTCACC TTGGTCAGCT 
TGGGGACCTT GTACTTATGA AAACTGTAAT GATCAGCAAG GGAAAAAAGG CTTCAAACTG 
AGGAAGCGGC GCATTACCAA TGAGCCCACT GGAGGCTCTG GGGTAACCGG AAACTGCCCT 
CACTTACTGG AAGCCATTCC CTGTGAAGAG CCTGCCTGTT ATGACTGGAA AGCAGTGAGA 
CTGGGAAACT GCGAGCCAGA TAACGGAAAG GAGTGTGGTC CAGGCACGCA AGTTCAAGAG 
GTTGTGTGCA TCAACAGTGA TGGAGAAGAA GTTGACAGAC AGCTGTGCAG AGATGCCATC 
TTCCCCATCC CTGTGGCCTG TGATGCCCCA TGCCCGAAAG ACTGTGTGCT CAGCACATGG 
TCTACGTGGT CCTCCTGCTC ACACACCTGC TCAGGGAAAA CGACAGAAGG GAAACAGATA 
CGAGCACGAT CCATTCTGGC CTATGCGGGT GAAGAAGGTG GAATTCGCTG TCCAAATAGC 
AGTGCTTTGC AAGAAGTACG AAGCTGTAAT GAGCATCCTT GCACAGTGTA CCACTGGCAA 
ACTGGTCCCT GGGGCCAGTG CATTGAGGAC ACCTCAGTAT CGTCCTTCAA CACAACTACG 
ACTTGGAATG GGGAGGCCTC CTGCTCTGTC GGCATGCAGA CAAGAAAAGT CATCTGTGTG 
CGAGTCAATG TGGGCCAAGT GGGACCCAAA AAATGTCCTG AAAGCCTTCG ACCTGAAACT 
GTAAGGCCTT GTCTGCTTCC TTGTAAGAAG GACTGTATTG TGACCCCATA TAGTGACTGG 
ACATCATGCC CCTCTTCGTG TAAAGAAGGG GACTCCAGTA TCAGGAAGCA GTCTAGGCAT 
CGGGTCATCA TTCAGCTGCC AGCCAACGGG GGCCGAGACT GCACAGATCC CCTCTATGAA 
GAGAAGGCCT GTGAGGCACC TCAAGCGTGC CAAAGCTACA GGTGGAAGAC TCACAAATGG 
CGCAGATGCC AATTAGTCCC TTGGAGCGTG CAACAAGACA GCCCTGGAGC ACAGGAAGGC 
TGTGGGCCTG GGCGACAGGC AAGAGCCATT ACTTGTCGCA AGCAAGATGG AGGACAGGCT 
GGAATCCATG AGTGCCTACA GTATGCAGGC CCTGTGCCAG CCCTTACCCA GGCCTGCCAG 
ATCCCCTGCC AGGATGACTG TCAATTGACC AGCTGGTCCA AGTTTTCTTC ATGCAATGGA 
GACTGTGGTG CAGTTAGGAC CAGAAAGCGC ACTCTTGTTG GAAAAAGTAA AAAGAAGGAA 
AAATGTAAAA ATTCCCATTT GTATCCCCTG ATTGAGACTC AGTATTGTCC TTGTGACAAA 
TATAATGCAC AACCTGTGGG GAACTGGTCA GACTGTATTT TACCAGAGGG AAAAGTGGAA 
GTGTTGCTGG GAATGAAAGT ACAAGGAGAC ATCAAGGAAT GCGGACAAGG ATATCGTTAC 
CAAGCAATGG CATGCTACGA TCAAAATGGC AGGCTTGTGG AAACATCTAG ATGTAACAGC 
CATGGTTACA TTGAGGAGGC CTGCATCATC CCCTGCCCCT CAGACTGCAA GCTCAGTGAG 
TGGTCCAACT GGTCGCGCTG CAGCAAGTCC TGTGGGAGTG GTGTGAAGGT TCGTTCTAAA 
TGGCTGCGTG AAAAACCATA TAATGGAGGA AGGCCTTGCC CCAAACTGGA CCATGTCAAC 
CAGGCACAGG TGTATGAGGT TGTCCCATGC CACAGTGACT GCAACCAGTA CCTATGGGTC 
ACAGAGCCCT GGAGCATCTG CAAGGTGACC TTTGTGAATA TGCGGGAGAA CTGTGGAGAG 
GGCGTGCAAA CCCGAAAAGT GAGATGCATG CAGAATACAG CAGATGGCCC TTCTGAACAT 
GTAGAGGATT ACCTCTGTGA CCCAGAAGAG ATGCCCCTGG GCTCTAGAGT GTGCAAATTA 
CCATGCCCTG AGGACTGTGT GATATCTGAA TGGGGTCCAT GGACCCAATG TGTTTTGCCT 
TGCAATCAAA GCAGTTTCCG GCAAAGGTCA GCTGATCCCA TCAGACAACC AGCTGATGAA 
GGAAGATCTT GCCCTAATGC TGTTGAGAAA GAACCCTGTA ACCTGAACAA AAACTGCTAC 
CACTATGATT ATAATGTAAC AGACTGGAGT ACATGTCAGC TGAGTGAGAA GGCAGTTTGT 
GGAAATGGAA TAAAAACAAG GATGTTGGAT TGTGTTCGAA GTGATGGCAA GTCAGTTGAC 
CTGAAATATT GTGAAGCGCT TGGCTTGGAG AAGAACTGGC AGATGAACAC GTCCTGCATG 
GTGGAATGCC CTGTGAACTG TCAGCTTTCT GATTGGTCTC CTTGGTCAGA ATGTTCTCAA 
ACATGTGGCC TCACAGGAAA AATGATCCGA AGACGAACAG TGACCCAGCC CTTTCAAGGT 
GATGGAAGAC CATGCCCTTC CCTGATGGAC CAGTCCAAAC CCTGCCCAGT GAAGCCTTGT 
TATCGGTGGC AATATGGCCA GTGGTCTCCA TGCCAAGTGC AGGAGGCCCA GTGTGGAGAA 
GGGACCAGAA CAAGGAACAT TTCTTGTGTA GTAAGTGATG GGTCAGCTGA TGATTTCAGC 
AAAGTGGTGG ATGAGGAATT CTGTGCTGAC ATTGAACTCA TTATAGATGG TAATAAAAAT 
ATGGTTCTGG AGGAATCCTG CAGCCAGCCT TGCCCAGGTG ACTGTTATTT GAAGGACTGG 
TCTTCCTGGA GCCTGTGTCA GCTGACCTGT GTGAATGGTG AGGATCTAGG CTTTGGTGGA 
ATACAGGTCA GATCCAGACC GGTGATTATA CAAGAACTAG AGAATCAGCA TCTGTGCCCA 
GAGCAGATGT TAGAAACAAA ATCATGTTAT GATGGACAGT GCTATGAATA TAAATGGATG 
GCCAGTGCTT GGAAGGGCTC TTCCCGAACA GTGTGGTGTC AAAGGTCAGA TGGTATAAAT 
GTAACAGCAA AAAGCCAAAG AAACCCCAAA GAAGGCAAAA CAACCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circulating Antibodies against Thrombospondin Type-I Domain-Containing 7A in Chinese Patients with Idiopathic Membranous Nephropathy.
Clinical journal of the American Society of Nephrology : CJASN12(10)1642-1651(2017 Oct)
Wang J,Cui Z,Lu J,Probst C,Zhang YM,Wang X,Qu Z,Wang F,Meng LQ,Cheng XY,Liu G,Debiec H,Ronco P,Zhao MH

Recent Progress in Deciphering the Etiopathogenesis of Primary Membranous Nephropathy.
BioMed research international20171936372(2017)
Kronbichler A,Oh J,Meijers B,Mayer G,Shin JI

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THSD7A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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