EZH1 cDNA ORF clone, Homo sapiens(human)

The following EZH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EZH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27740 NM_001991.5
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu50206 NM_001321082.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu57643 XM_017024352.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu86690 XM_017024351.1
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu50204 NM_001321079.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57642 NM_001321081.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu50205 XM_005257145.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57641 XM_011524517.2
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu86689 XM_017024350.1
Latest version!
Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu50204 NM_001321079.1 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu27740 NM_001991.4 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu50206 NM_001321082.1 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu27740 NM_001991.3 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu57642 NM_001321081.1 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57643 XM_017024352.1 Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27740
    Clone ID Related Accession (Same CDS sequence) NM_001991.4 , NM_001991.5 , NM_001991.3
    Accession Version NM_001991.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-11
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC423942.1, DC333017.1, AB002386.1 and AL833159.1. On Mar 18, 2016 this sequence version replaced NM_001991.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015882.1, SRR1803617.9767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001982.2
    CDS189..2432
    Translation

    Target ORF information:

    RefSeq Version NM_001991.4
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001991.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27740
    Clone ID Related Accession (Same CDS sequence) NM_001991.4 , NM_001991.5 , NM_001991.3
    Accession Version NM_001991.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC333017.1, AB002386.1 and AL833159.1. On Nov 23, 2018 this sequence version replaced NM_001991.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015882.1, SRR1660807.85875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000428826.7/ ENSP00000404658.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001982.2
    CDS133..2376
    Translation

    Target ORF information:

    RefSeq Version NM_001991.5
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001991.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27740
    Clone ID Related Accession (Same CDS sequence) NM_001991.4 , NM_001991.5 , NM_001991.3
    Accession Version NM_001991.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product histone-lysine N-methyltransferase EZH1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001982.2
    Translation

    Target ORF information:

    RefSeq Version NM_001991.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001991.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAACATAT GTTAATGAGG
    TCACTGAACA CAGTTGCATT GGTTCCCATC ATGTATTCCT GGTCCCCTCT CCAACAGAAC
    TTTATGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50206
    Clone ID Related Accession (Same CDS sequence) NM_001321082.1 , NM_001321082.2
    Accession Version NM_001321082.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-22
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC423942.1, AK299887.1, AB002386.1 and AL833159.1. On Mar 18, 2016 this sequence version replaced XM_005257146.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.206673.1, SRR1803614.45525.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308011.1
    CDS189..2312
    Translation

    Target ORF information:

    RefSeq Version NM_001321082.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50206
    Clone ID Related Accession (Same CDS sequence) NM_001321082.1 , NM_001321082.2
    Accession Version NM_001321082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC423942.1, AK299887.1, AB002386.1 and AL833159.1. On May 31, 2019 this sequence version replaced NM_001321082.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.113240.1, SRR1803615.241495.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308011.1
    CDS133..2256
    Translation

    Target ORF information:

    RefSeq Version NM_001321082.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTG TGAGTGGACA CCCTTTTCTC
    AAAAAGGTAG AAGATGAGAC GGTTTTGTGC AATATTCCCT ACATGGGAGA TGAAGTGAAA
    GAAGAAGATG AGACTTTTAT TGAGGAGCTG ATCAATAACT ATGATGGGAA AGTCCATGGT
    GAAGAAGAGA TGATCCCTGG ATCCGTTCTG ATTAGTGATG CTGTTTTTCT GGAGTTGGTC
    GATGCCCTGA ATCAGTACTC AGATGAGGAG GAGGAAGGGC ACAATGACAC CTCAGATGGA
    AAGCAGGATG ACAGCAAAGA AGATCTGCCA GTAACAAGAA AGAGAAAGCG ACATGCTATT
    GAAGGCAACA AAAAGAGTTC CAAGAAACAG TTCCCAAATG ACATGATCTT CAGTGCAATT
    GCCTCAATGT TCCCTGAGAA TGGTGTCCCA GATGACATGA AGGAGAGGTA TCGAGAACTA
    ACAGAGATGT CAGACCCCAA TGCACTTCCC CCTCAGTGCA CACCCAACAT CGATGGCCCC
    AATGCCAAGT CTGTGCAGCG GGAGCAATCT CTGCACTCCT TCCACACACT TTTTTGCCGG
    CGCTGCTTTA AATACGACTG CTTCCTTCAC CCTTTTCATG CCACCCCTAA TGTATATAAA
    CGCAAGAATA AAGAAATCAA GATTGAACCA GAACCATGTG GCACAGACTG CTTCCTTTTG
    CTGGAAGGAG CAAAGGAGTA TGCCATGCTC CACAACCCCC GCTCCAAGTG CTCTGGTCGT
    CGCCGGAGAA GGCACCACAT AGTCAGTGCT TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG
    GCTGAGACTA AAGAAGGAGA CAGTGACAGG GACACAGGCA ATGACTGGGC CTCCAGTTCT
    TCAGAGGCTA ACTCTCGCTG TCAGACTCCC ACAAAACAGA AGGCTAGTCC AGCCCCACCT
    CAACTCTGCG TAGTGGAAGC ACCCTCGGAG CCTGTGGAAT GGACTGGGGC TGAAGAATCT
    CTTTTTCGAG TCTTCCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGGCTTCTG
    GGGACCAAGA CGTGCAAGCA GGTCTTTCAG TTTGCAGTCA AAGAATCACT TATCCTGAAG
    CTGCCAACAG ATGAGCTCAT GAACCCCTCA CAGAAGAAGA AAAGAAAGCA CAGATTGTGG
    GCTGCACACT GCAGGAAGAT TCAGCTGAAG AAAGATAACT CTTCCACACA AGTGTACAAC
    TACCAACCCT GCGACCACCC AGACCGCCCC TGTGACAGCA CCTGCCCCTG CATCATGACT
    CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCAGACT GTCAGAATCG TTTCCCTGGC
    TGTCGCTGTA AGACCCAGTG CAATACCAAG CAATGTCCTT GCTATCTGGC AGTGCGAGAA
    TGTGACCCTG ACCTGTGTCT CACCTGTGGG GCCTCAGAGC ACTGGGACTG CAAGGTGGTT
    TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT
    GATGTGGCCG GATGGGGCAC CTTCATAAAG GAGTCTGTGC AGAAGAACGA ATTCATTTCT
    GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCTGATC GACGCGGAAA GGTCTATGAC
    AAATACATGT CCAGCTTCCT CTTCAACCTC AATAATGATT TTGTAGTGGA TGCTACTCGG
    AAAGGAAACA AAATTCGATT TGCAAATCAT TCAGTGAATC CCAACTGTTA TGCCAAAGTG
    GTCATGGTGA ATGGAGACCA TCGGATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC
    GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CTCTCAAGTA CGTGGGGATC
    GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57643
    Clone ID Related Accession (Same CDS sequence) XM_017024352.1 , XM_017024352.2
    Accession Version XM_017024352.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTATTGA AGGAAGGAGC AAAGGAGTAT GCCATGCTCC ACAACCCCCG CTCCAAGTGC 
    TCTGGTCGTC GCCGGAGAAG GCACCACATA GTCAGTGCTT CCTGCTCCAA TGCCTCAGCC
    TCTGCTGTGG CTGAGACTAA AGAAGGAGAC AGTGACAGGG ACACAGGCAA TGACTGGGCC
    TCCAGTTCTT CAGAGGCTAA CTCTCGCTGT CAGACTCCCA CAAAACAGAA GGCTAGTCCA
    GCCCCACCTC AACTCTGCGT AGTGGAAGCA CCCTCGGAGC CTGTGGAATG GACTGGGGCT
    GAAGAATCTC TTTTTCGAGT CTTCCATGGC ACCTACTTCA ACAACTTCTG TTCAATAGCC
    AGGCTTCTGG GGACCAAGAC GTGCAAGCAG GTCTTTCAGT TTGCAGTCAA AGAATCACTT
    ATCCTGAAGC TGCCAACAGA TGAGCTCATG AACCCCTCAC AGAAGAAGAA AAGAAAGCAC
    AGATTGTGGG CTGCACACTG CAGGAAGATT CAGCTGAAGA AAGATAACTC TTCCACACAA
    GTGTACAACT ACCAACCCTG CGACCACCCA GACCGCCCCT GTGACAGCAC CTGCCCCTGC
    ATCATGACTC AGAATTTCTG TGAGAAGTTC TGCCAGTGCA ACCCAGACTG TCAGAATCGT
    TTCCCTGGCT GTCGCTGTAA GACCCAGTGC AATACCAAGC AATGTCCTTG CTATCTGGCA
    GTGCGAGAAT GTGACCCTGA CCTGTGTCTC ACCTGTGGGG CCTCAGAGCA CTGGGACTGC
    AAGGTGGTTT CCTGTAAAAA CTGCAGCATC CAGCGTGGAC TTAAGAAGCA CCTGCTGCTG
    GCCCCCTCTG ATGTGGCCGG ATGGGGCACC TTCATAAAGG AGTCTGTGCA GAAGAACGAA
    TTCATTTCTG AATACTGTGG TGAGCTCATC TCTCAGGATG AGGCTGATCG ACGCGGAAAG
    GTCTATGACA AATACATGTC CAGCTTCCTC TTCAACCTCA ATAATGATTT TGTAGTGGAT
    GCTACTCGGA AAGGAAACAA AATTCGATTT GCAAATCATT CAGTGAATCC CAACTGTTAT
    GCCAAAGTGG TCATGGTGAA TGGAGACCAT CGGATTGGGA TCTTTGCCAA GAGGGCAATT
    CAAGCTGGCG AAGAGCTCTT CTTTGATTAC AGGTACAGCC AAGCTGATGC TCTCAAGTAC
    GTGGGGATCG AGAGGGAGAC CGACGTCCTT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879841.1
    CDS785..2017
    Translation

    Target ORF information:

    RefSeq Version XM_017024352.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTATTGA AGGAAGGAGC AAAGGAGTAT GCCATGCTCC ACAACCCCCG CTCCAAGTGC 
    TCTGGTCGTC GCCGGAGAAG GCACCACATA GTCAGTGCTT CCTGCTCCAA TGCCTCAGCC
    TCTGCTGTGG CTGAGACTAA AGAAGGAGAC AGTGACAGGG ACACAGGCAA TGACTGGGCC
    TCCAGTTCTT CAGAGGCTAA CTCTCGCTGT CAGACTCCCA CAAAACAGAA GGCTAGTCCA
    GCCCCACCTC AACTCTGCGT AGTGGAAGCA CCCTCGGAGC CTGTGGAATG GACTGGGGCT
    GAAGAATCTC TTTTTCGAGT CTTCCATGGC ACCTACTTCA ACAACTTCTG TTCAATAGCC
    AGGCTTCTGG GGACCAAGAC GTGCAAGCAG GTCTTTCAGT TTGCAGTCAA AGAATCACTT
    ATCCTGAAGC TGCCAACAGA TGAGCTCATG AACCCCTCAC AGAAGAAGAA AAGAAAGCAC
    AGATTGTGGG CTGCACACTG CAGGAAGATT CAGCTGAAGA AAGATAACTC TTCCACACAA
    GTGTACAACT ACCAACCCTG CGACCACCCA GACCGCCCCT GTGACAGCAC CTGCCCCTGC
    ATCATGACTC AGAATTTCTG TGAGAAGTTC TGCCAGTGCA ACCCAGACTG TCAGAATCGT
    TTCCCTGGCT GTCGCTGTAA GACCCAGTGC AATACCAAGC AATGTCCTTG CTATCTGGCA
    GTGCGAGAAT GTGACCCTGA CCTGTGTCTC ACCTGTGGGG CCTCAGAGCA CTGGGACTGC
    AAGGTGGTTT CCTGTAAAAA CTGCAGCATC CAGCGTGGAC TTAAGAAGCA CCTGCTGCTG
    GCCCCCTCTG ATGTGGCCGG ATGGGGCACC TTCATAAAGG AGTCTGTGCA GAAGAACGAA
    TTCATTTCTG AATACTGTGG TGAGCTCATC TCTCAGGATG AGGCTGATCG ACGCGGAAAG
    GTCTATGACA AATACATGTC CAGCTTCCTC TTCAACCTCA ATAATGATTT TGTAGTGGAT
    GCTACTCGGA AAGGAAACAA AATTCGATTT GCAAATCATT CAGTGAATCC CAACTGTTAT
    GCCAAAGTGG TCATGGTGAA TGGAGACCAT CGGATTGGGA TCTTTGCCAA GAGGGCAATT
    CAAGCTGGCG AAGAGCTCTT CTTTGATTAC AGGTACAGCC AAGCTGATGC TCTCAAGTAC
    GTGGGGATCG AGAGGGAGAC CGACGTCCTT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57643
    Clone ID Related Accession (Same CDS sequence) XM_017024352.1 , XM_017024352.2
    Accession Version XM_017024352.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTATTGA AGGAAGGAGC AAAGGAGTAT GCCATGCTCC ACAACCCCCG CTCCAAGTGC 
    TCTGGTCGTC GCCGGAGAAG GCACCACATA GTCAGTGCTT CCTGCTCCAA TGCCTCAGCC
    TCTGCTGTGG CTGAGACTAA AGAAGGAGAC AGTGACAGGG ACACAGGCAA TGACTGGGCC
    TCCAGTTCTT CAGAGGCTAA CTCTCGCTGT CAGACTCCCA CAAAACAGAA GGCTAGTCCA
    GCCCCACCTC AACTCTGCGT AGTGGAAGCA CCCTCGGAGC CTGTGGAATG GACTGGGGCT
    GAAGAATCTC TTTTTCGAGT CTTCCATGGC ACCTACTTCA ACAACTTCTG TTCAATAGCC
    AGGCTTCTGG GGACCAAGAC GTGCAAGCAG GTCTTTCAGT TTGCAGTCAA AGAATCACTT
    ATCCTGAAGC TGCCAACAGA TGAGCTCATG AACCCCTCAC AGAAGAAGAA AAGAAAGCAC
    AGATTGTGGG CTGCACACTG CAGGAAGATT CAGCTGAAGA AAGATAACTC TTCCACACAA
    GTGTACAACT ACCAACCCTG CGACCACCCA GACCGCCCCT GTGACAGCAC CTGCCCCTGC
    ATCATGACTC AGAATTTCTG TGAGAAGTTC TGCCAGTGCA ACCCAGACTG TCAGAATCGT
    TTCCCTGGCT GTCGCTGTAA GACCCAGTGC AATACCAAGC AATGTCCTTG CTATCTGGCA
    GTGCGAGAAT GTGACCCTGA CCTGTGTCTC ACCTGTGGGG CCTCAGAGCA CTGGGACTGC
    AAGGTGGTTT CCTGTAAAAA CTGCAGCATC CAGCGTGGAC TTAAGAAGCA CCTGCTGCTG
    GCCCCCTCTG ATGTGGCCGG ATGGGGCACC TTCATAAAGG AGTCTGTGCA GAAGAACGAA
    TTCATTTCTG AATACTGTGG TGAGCTCATC TCTCAGGATG AGGCTGATCG ACGCGGAAAG
    GTCTATGACA AATACATGTC CAGCTTCCTC TTCAACCTCA ATAATGATTT TGTAGTGGAT
    GCTACTCGGA AAGGAAACAA AATTCGATTT GCAAATCATT CAGTGAATCC CAACTGTTAT
    GCCAAAGTGG TCATGGTGAA TGGAGACCAT CGGATTGGGA TCTTTGCCAA GAGGGCAATT
    CAAGCTGGCG AAGAGCTCTT CTTTGATTAC AGGTACAGCC AAGCTGATGC TCTCAAGTAC
    GTGGGGATCG AGAGGGAGAC CGACGTCCTT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879841.1
    CDS787..2019
    Translation

    Target ORF information:

    RefSeq Version XM_017024352.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024352.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTATTGA AGGAAGGAGC AAAGGAGTAT GCCATGCTCC ACAACCCCCG CTCCAAGTGC 
    TCTGGTCGTC GCCGGAGAAG GCACCACATA GTCAGTGCTT CCTGCTCCAA TGCCTCAGCC
    TCTGCTGTGG CTGAGACTAA AGAAGGAGAC AGTGACAGGG ACACAGGCAA TGACTGGGCC
    TCCAGTTCTT CAGAGGCTAA CTCTCGCTGT CAGACTCCCA CAAAACAGAA GGCTAGTCCA
    GCCCCACCTC AACTCTGCGT AGTGGAAGCA CCCTCGGAGC CTGTGGAATG GACTGGGGCT
    GAAGAATCTC TTTTTCGAGT CTTCCATGGC ACCTACTTCA ACAACTTCTG TTCAATAGCC
    AGGCTTCTGG GGACCAAGAC GTGCAAGCAG GTCTTTCAGT TTGCAGTCAA AGAATCACTT
    ATCCTGAAGC TGCCAACAGA TGAGCTCATG AACCCCTCAC AGAAGAAGAA AAGAAAGCAC
    AGATTGTGGG CTGCACACTG CAGGAAGATT CAGCTGAAGA AAGATAACTC TTCCACACAA
    GTGTACAACT ACCAACCCTG CGACCACCCA GACCGCCCCT GTGACAGCAC CTGCCCCTGC
    ATCATGACTC AGAATTTCTG TGAGAAGTTC TGCCAGTGCA ACCCAGACTG TCAGAATCGT
    TTCCCTGGCT GTCGCTGTAA GACCCAGTGC AATACCAAGC AATGTCCTTG CTATCTGGCA
    GTGCGAGAAT GTGACCCTGA CCTGTGTCTC ACCTGTGGGG CCTCAGAGCA CTGGGACTGC
    AAGGTGGTTT CCTGTAAAAA CTGCAGCATC CAGCGTGGAC TTAAGAAGCA CCTGCTGCTG
    GCCCCCTCTG ATGTGGCCGG ATGGGGCACC TTCATAAAGG AGTCTGTGCA GAAGAACGAA
    TTCATTTCTG AATACTGTGG TGAGCTCATC TCTCAGGATG AGGCTGATCG ACGCGGAAAG
    GTCTATGACA AATACATGTC CAGCTTCCTC TTCAACCTCA ATAATGATTT TGTAGTGGAT
    GCTACTCGGA AAGGAAACAA AATTCGATTT GCAAATCATT CAGTGAATCC CAACTGTTAT
    GCCAAAGTGG TCATGGTGAA TGGAGACCAT CGGATTGGGA TCTTTGCCAA GAGGGCAATT
    CAAGCTGGCG AAGAGCTCTT CTTTGATTAC AGGTACAGCC AAGCTGATGC TCTCAAGTAC
    GTGGGGATCG AGAGGGAGAC CGACGTCCTT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86690
    Clone ID Related Accession (Same CDS sequence) XM_017024351.1
    Accession Version XM_017024351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGCCTG TGAGTGGACA CCCTTTTCTC AAAAAGGTAG AAGATGAGAC GGTTTTGTGC 
    AATATTCCCT ACATGGGAGA TGAAGTGAAA GAAGAAGATG AGACTTTTAT TGAGGAGCTG
    ATCAATAACT ATGATGGGAA AGTCCATGGT GAAGAAGAGA TGATCCCTGG ATCCGTTCTG
    ATTAGTGATG CTGTTTTTCT GGAGTTGGTC GATGCCCTGA ATCAGTACTC AGATGAGGAG
    GAGGAAGGGC ACAATGACAC CTCAGATGGA AAGCAGGATG ACAGCAAAGA AGATCTGCCA
    GTAACAAGAA AGAGAAAGCG ACATGCTATT GAAGGCAACA AAAAGAGTTC CAAGAAACAG
    TTCCCAAATG ACATGATCTT CAGTGCAATT GCCTCAATGT TCCCTGAGAA TGGTGTCCCA
    GATGACATGA AGGAGAGGTA TCGAGAACTA ACAGAGATGT CAGACCCCAA TGCACTTCCC
    CCTCAGTGCA CACCCAACAT CGATGGCCCC AATGCCAAGT CTGTGCAGCG GGAGCAATCT
    CTGCACTCCT TCCACACACT TTTTTGCCGG CGCTGCTTTA AATACGACTG CTTCCTTCAC
    CCTTTTCATG CCACCCCTAA TGTATATAAA CGCAAGAATA AAGAAATCAA GATTGAACCA
    GAACCATGTG GCACAGACTG CTTCCTTTTG CTGGAAGGAG CAAAGGAGTA TGCCATGCTC
    CACAACCCCC GCTCCAAGTG CTCTGGTCGT CGCCGGAGAA GGCACCACAT AGTCAGTGCT
    TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG GCTGAGACTA AAGAAGGAGA CAGTGACAGG
    GACACAGGCA ATGACTGGGC CTCCAGTTCT TCAGAGGCTA ACTCTCGCTG TCAGACTCCC
    ACAAAACAGA AGGCTAGTCC AGCCCCACCT CAACTCTGCG TAGTGGAAGC ACCCTCGGAG
    CCTGTGGAAT GGACTGGGGC TGAAGAATCT CTTTTTCGAG TCTTCCATGG CACCTACTTC
    AACAACTTCT GTTCAATAGC CAGGCTTCTG GGGACCAAGA CGTGCAAGCA GGTCTTTCAG
    TTTGCAGTCA AAGAATCACT TATCCTGAAG CTGCCAACAG ATGAGCTCAT GAACCCCTCA
    CAGAAGAAGA AAAGAAAGCA CAGATTGTGG GCTGCACACT GCAGGAAGAT TCAGCTGAAG
    AAAGATAACT CTTCCACACA AGTGTACAAC TACCAACCCT GCGACCACCC AGACCGCCCC
    TGTGACAGCA CCTGCCCCTG CATCATGACT CAGAATTTCT GTGAGAAGTT CTGCCAGTGC
    AACCCAGACT GTCAGAATCG TTTCCCTGGC TGTCGCTGTA AGACCCAGTG CAATACCAAG
    CAATGTCCTT GCTATCTGGC AGTGCGAGAA TGTGACCCTG ACCTGTGTCT CACCTGTGGG
    GCCTCAGAGC ACTGGGACTG CAAGGTGGTT TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA
    CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT GATGTGGCCG GATGGGGCAC CTTCATAAAG
    GAGTCTGTGC AGAAGAACGA ATTCATTTCT GAATACTGTG GTGAGCTCAT CTCTCAGGAT
    GAGGCTGATC GACGCGGAAA GGTCTATGAC AAATACATGT CCAGCTTCCT CTTCAACCTC
    AATAATGATT TTGTAGTGGA TGCTACTCGG AAAGGAAACA AAATTCGATT TGCAAATCAT
    TCAGTGAATC CCAACTGTTA TGCCAAAGTG GTCATGGTGA ATGGAGACCA TCGGATTGGG
    ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC GAAGAGCTCT TCTTTGATTA CAGGTACAGC
    CAAGCTGATG CTCTCAAGTA CGTGGGGATC GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879840.1
    CDS253..2166
    Translation

    Target ORF information:

    RefSeq Version XM_017024351.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAGCCTG TGAGTGGACA CCCTTTTCTC AAAAAGGTAG AAGATGAGAC GGTTTTGTGC 
    AATATTCCCT ACATGGGAGA TGAAGTGAAA GAAGAAGATG AGACTTTTAT TGAGGAGCTG
    ATCAATAACT ATGATGGGAA AGTCCATGGT GAAGAAGAGA TGATCCCTGG ATCCGTTCTG
    ATTAGTGATG CTGTTTTTCT GGAGTTGGTC GATGCCCTGA ATCAGTACTC AGATGAGGAG
    GAGGAAGGGC ACAATGACAC CTCAGATGGA AAGCAGGATG ACAGCAAAGA AGATCTGCCA
    GTAACAAGAA AGAGAAAGCG ACATGCTATT GAAGGCAACA AAAAGAGTTC CAAGAAACAG
    TTCCCAAATG ACATGATCTT CAGTGCAATT GCCTCAATGT TCCCTGAGAA TGGTGTCCCA
    GATGACATGA AGGAGAGGTA TCGAGAACTA ACAGAGATGT CAGACCCCAA TGCACTTCCC
    CCTCAGTGCA CACCCAACAT CGATGGCCCC AATGCCAAGT CTGTGCAGCG GGAGCAATCT
    CTGCACTCCT TCCACACACT TTTTTGCCGG CGCTGCTTTA AATACGACTG CTTCCTTCAC
    CCTTTTCATG CCACCCCTAA TGTATATAAA CGCAAGAATA AAGAAATCAA GATTGAACCA
    GAACCATGTG GCACAGACTG CTTCCTTTTG CTGGAAGGAG CAAAGGAGTA TGCCATGCTC
    CACAACCCCC GCTCCAAGTG CTCTGGTCGT CGCCGGAGAA GGCACCACAT AGTCAGTGCT
    TCCTGCTCCA ATGCCTCAGC CTCTGCTGTG GCTGAGACTA AAGAAGGAGA CAGTGACAGG
    GACACAGGCA ATGACTGGGC CTCCAGTTCT TCAGAGGCTA ACTCTCGCTG TCAGACTCCC
    ACAAAACAGA AGGCTAGTCC AGCCCCACCT CAACTCTGCG TAGTGGAAGC ACCCTCGGAG
    CCTGTGGAAT GGACTGGGGC TGAAGAATCT CTTTTTCGAG TCTTCCATGG CACCTACTTC
    AACAACTTCT GTTCAATAGC CAGGCTTCTG GGGACCAAGA CGTGCAAGCA GGTCTTTCAG
    TTTGCAGTCA AAGAATCACT TATCCTGAAG CTGCCAACAG ATGAGCTCAT GAACCCCTCA
    CAGAAGAAGA AAAGAAAGCA CAGATTGTGG GCTGCACACT GCAGGAAGAT TCAGCTGAAG
    AAAGATAACT CTTCCACACA AGTGTACAAC TACCAACCCT GCGACCACCC AGACCGCCCC
    TGTGACAGCA CCTGCCCCTG CATCATGACT CAGAATTTCT GTGAGAAGTT CTGCCAGTGC
    AACCCAGACT GTCAGAATCG TTTCCCTGGC TGTCGCTGTA AGACCCAGTG CAATACCAAG
    CAATGTCCTT GCTATCTGGC AGTGCGAGAA TGTGACCCTG ACCTGTGTCT CACCTGTGGG
    GCCTCAGAGC ACTGGGACTG CAAGGTGGTT TCCTGTAAAA ACTGCAGCAT CCAGCGTGGA
    CTTAAGAAGC ACCTGCTGCT GGCCCCCTCT GATGTGGCCG GATGGGGCAC CTTCATAAAG
    GAGTCTGTGC AGAAGAACGA ATTCATTTCT GAATACTGTG GTGAGCTCAT CTCTCAGGAT
    GAGGCTGATC GACGCGGAAA GGTCTATGAC AAATACATGT CCAGCTTCCT CTTCAACCTC
    AATAATGATT TTGTAGTGGA TGCTACTCGG AAAGGAAACA AAATTCGATT TGCAAATCAT
    TCAGTGAATC CCAACTGTTA TGCCAAAGTG GTCATGGTGA ATGGAGACCA TCGGATTGGG
    ATCTTTGCCA AGAGGGCAAT TCAAGCTGGC GAAGAGCTCT TCTTTGATTA CAGGTACAGC
    CAAGCTGATG CTCTCAAGTA CGTGGGGATC GAGAGGGAGA CCGACGTCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50204
    Clone ID Related Accession (Same CDS sequence) NM_001321079.1 , NM_001321079.2
    Accession Version NM_001321079.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-22
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC423942.1, AK295626.1, AB002386.1 and AL833159.1. On Mar 18, 2016 this sequence version replaced XM_005257144.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.157434.1, SRR1803612.172850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGTGTACC ATAGAGAGCA TTTTCCCGGG ATTTGCAAGC
    CAACATATGT TAATGAGGTC ACTGAACACA GTTGCATTGG TTCCCATCAT GTATTCCTGG
    TCCCCTCTCC AACAGAACTT TATGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308008.1
    CDS80..2341
    Translation

    Target ORF information:

    RefSeq Version NM_001321079.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGTGTACC ATAGAGAGCA TTTTCCCGGG ATTTGCAAGC
    CAACATATGT TAATGAGGTC ACTGAACACA GTTGCATTGG TTCCCATCAT GTATTCCTGG
    TCCCCTCTCC AACAGAACTT TATGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50204
    Clone ID Related Accession (Same CDS sequence) NM_001321079.1 , NM_001321079.2
    Accession Version NM_001321079.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295626.1, AB002386.1 and AL833159.1. On May 31, 2019 this sequence version replaced NM_001321079.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.172850.1, SRR1660807.27572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGTGTACC ATAGAGAGCA TTTTCCCGGG ATTTGCAAGC
    CAACATATGT TAATGAGGTC ACTGAACACA GTTGCATTGG TTCCCATCAT GTATTCCTGG
    TCCCCTCTCC AACAGAACTT TATGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308008.1
    CDS24..2285
    Translation

    Target ORF information:

    RefSeq Version NM_001321079.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321079.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGTGTACC ATAGAGAGCA TTTTCCCGGG ATTTGCAAGC
    CAACATATGT TAATGAGGTC ACTGAACACA GTTGCATTGG TTCCCATCAT GTATTCCTGG
    TCCCCTCTCC AACAGAACTT TATGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57642
    Clone ID Related Accession (Same CDS sequence) NM_001321081.1 , NM_001321081.2
    Accession Version NM_001321081.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-22
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC423942.1, AL833159.1 and AB002386.1. On Mar 18, 2016 this sequence version replaced XM_011524518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL833159.1, SRR1803617.93587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTT GTACCATAGA GAGCATTTTC
    CCGGGATTTG CAAGCCAACA TATGTTAATG AGGTCACTGA ACACAGTTGC ATTGGTTCCC
    ATCATGTATT CCTGGTCCCC TCTCCAACAG AACTTTATGG TAGAAGATGA GACGGTTTTG
    TGCAATATTC CCTACATGGG AGATGAAGTG AAAGAAGAAG ATGAGACTTT TATTGAGGAG
    CTGATCAATA ACTATGATGG GAAAGTCCAT GGTGAAGAAG AGATGATCCC TGGATCCGTT
    CTGATTAGTG ATGCTGTTTT TCTGGAGTTG GTCGATGCCC TGAATCAGTA CTCAGATGAG
    GAGGAGGAAG GGCACAATGA CACCTCAGAT GGAAAGCAGG ATGACAGCAA AGAAGATCTG
    CCAGTAACAA GAAAGAGAAA GCGACATGCT ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA
    CAGTTCCCAA ATGACATGAT CTTCAGTGCA ATTGCCTCAA TGTTCCCTGA GAATGGTGTC
    CCAGATGACA TGAAGGAGAG GTATCGAGAA CTAACAGAGA TGTCAGACCC CAATGCACTT
    CCCCCTCAGT GCACACCCAA CATCGATGGC CCCAATGCCA AGTCTGTGCA GCGGGAGCAA
    TCTCTGCACT CCTTCCACAC ACTTTTTTGC CGGCGCTGCT TTAAATACGA CTGCTTCCTT
    CACCCTTTTC ATGCCACCCC TAATGTATAT AAACGCAAGA ATAAAGAAAT CAAGATTGAA
    CCAGAACCAT GTGGCACAGA CTGCTTCCTT TTGCTGGAAG GAGCAAAGGA GTATGCCATG
    CTCCACAACC CCCGCTCCAA GTGCTCTGGT CGTCGCCGGA GAAGGCACCA CATAGTCAGT
    GCTTCCTGCT CCAATGCCTC AGCCTCTGCT GTGGCTGAGA CTAAAGAAGG AGACAGTGAC
    AGGGACACAG GCAATGACTG GGCCTCCAGT TCTTCAGAGG CTAACTCTCG CTGTCAGACT
    CCCACAAAAC AGAAGGCTAG TCCAGCCCCA CCTCAACTCT GCGTAGTGGA AGCACCCTCG
    GAGCCTGTGG AATGGACTGG GGCTGAAGAA TCTCTTTTTC GAGTCTTCCA TGGCACCTAC
    TTCAACAACT TCTGTTCAAT AGCCAGGCTT CTGGGGACCA AGACGTGCAA GCAGGTCTTT
    CAGTTTGCAG TCAAAGAATC ACTTATCCTG AAGCTGCCAA CAGATGAGCT CATGAACCCC
    TCACAGAAGA AGAAAAGAAA GCACAGATTG TGGGCTGCAC ACTGCAGGAA GATTCAGCTG
    AAGAAAGATA ACTCTTCCAC ACAAGTGTAC AACTACCAAC CCTGCGACCA CCCAGACCGC
    CCCTGTGACA GCACCTGCCC CTGCATCATG ACTCAGAATT TCTGTGAGAA GTTCTGCCAG
    TGCAACCCAG ACTGTCAGAA TCGTTTCCCT GGCTGTCGCT GTAAGACCCA GTGCAATACC
    AAGCAATGTC CTTGCTATCT GGCAGTGCGA GAATGTGACC CTGACCTGTG TCTCACCTGT
    GGGGCCTCAG AGCACTGGGA CTGCAAGGTG GTTTCCTGTA AAAACTGCAG CATCCAGCGT
    GGACTTAAGA AGCACCTGCT GCTGGCCCCC TCTGATGTGG CCGGATGGGG CACCTTCATA
    AAGGAGTCTG TGCAGAAGAA CGAATTCATT TCTGAATACT GTGGTGAGCT CATCTCTCAG
    GATGAGGCTG ATCGACGCGG AAAGGTCTAT GACAAATACA TGTCCAGCTT CCTCTTCAAC
    CTCAATAATG ATTTTGTAGT GGATGCTACT CGGAAAGGAA ACAAAATTCG ATTTGCAAAT
    CATTCAGTGA ATCCCAACTG TTATGCCAAA GTGGTCATGG TGAATGGAGA CCATCGGATT
    GGGATCTTTG CCAAGAGGGC AATTCAAGCT GGCGAAGAGC TCTTCTTTGA TTACAGGTAC
    AGCCAAGCTG ATGCTCTCAA GTACGTGGGG ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308010.1
    CDS189..2405
    Translation

    Target ORF information:

    RefSeq Version NM_001321081.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTT GTACCATAGA GAGCATTTTC
    CCGGGATTTG CAAGCCAACA TATGTTAATG AGGTCACTGA ACACAGTTGC ATTGGTTCCC
    ATCATGTATT CCTGGTCCCC TCTCCAACAG AACTTTATGG TAGAAGATGA GACGGTTTTG
    TGCAATATTC CCTACATGGG AGATGAAGTG AAAGAAGAAG ATGAGACTTT TATTGAGGAG
    CTGATCAATA ACTATGATGG GAAAGTCCAT GGTGAAGAAG AGATGATCCC TGGATCCGTT
    CTGATTAGTG ATGCTGTTTT TCTGGAGTTG GTCGATGCCC TGAATCAGTA CTCAGATGAG
    GAGGAGGAAG GGCACAATGA CACCTCAGAT GGAAAGCAGG ATGACAGCAA AGAAGATCTG
    CCAGTAACAA GAAAGAGAAA GCGACATGCT ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA
    CAGTTCCCAA ATGACATGAT CTTCAGTGCA ATTGCCTCAA TGTTCCCTGA GAATGGTGTC
    CCAGATGACA TGAAGGAGAG GTATCGAGAA CTAACAGAGA TGTCAGACCC CAATGCACTT
    CCCCCTCAGT GCACACCCAA CATCGATGGC CCCAATGCCA AGTCTGTGCA GCGGGAGCAA
    TCTCTGCACT CCTTCCACAC ACTTTTTTGC CGGCGCTGCT TTAAATACGA CTGCTTCCTT
    CACCCTTTTC ATGCCACCCC TAATGTATAT AAACGCAAGA ATAAAGAAAT CAAGATTGAA
    CCAGAACCAT GTGGCACAGA CTGCTTCCTT TTGCTGGAAG GAGCAAAGGA GTATGCCATG
    CTCCACAACC CCCGCTCCAA GTGCTCTGGT CGTCGCCGGA GAAGGCACCA CATAGTCAGT
    GCTTCCTGCT CCAATGCCTC AGCCTCTGCT GTGGCTGAGA CTAAAGAAGG AGACAGTGAC
    AGGGACACAG GCAATGACTG GGCCTCCAGT TCTTCAGAGG CTAACTCTCG CTGTCAGACT
    CCCACAAAAC AGAAGGCTAG TCCAGCCCCA CCTCAACTCT GCGTAGTGGA AGCACCCTCG
    GAGCCTGTGG AATGGACTGG GGCTGAAGAA TCTCTTTTTC GAGTCTTCCA TGGCACCTAC
    TTCAACAACT TCTGTTCAAT AGCCAGGCTT CTGGGGACCA AGACGTGCAA GCAGGTCTTT
    CAGTTTGCAG TCAAAGAATC ACTTATCCTG AAGCTGCCAA CAGATGAGCT CATGAACCCC
    TCACAGAAGA AGAAAAGAAA GCACAGATTG TGGGCTGCAC ACTGCAGGAA GATTCAGCTG
    AAGAAAGATA ACTCTTCCAC ACAAGTGTAC AACTACCAAC CCTGCGACCA CCCAGACCGC
    CCCTGTGACA GCACCTGCCC CTGCATCATG ACTCAGAATT TCTGTGAGAA GTTCTGCCAG
    TGCAACCCAG ACTGTCAGAA TCGTTTCCCT GGCTGTCGCT GTAAGACCCA GTGCAATACC
    AAGCAATGTC CTTGCTATCT GGCAGTGCGA GAATGTGACC CTGACCTGTG TCTCACCTGT
    GGGGCCTCAG AGCACTGGGA CTGCAAGGTG GTTTCCTGTA AAAACTGCAG CATCCAGCGT
    GGACTTAAGA AGCACCTGCT GCTGGCCCCC TCTGATGTGG CCGGATGGGG CACCTTCATA
    AAGGAGTCTG TGCAGAAGAA CGAATTCATT TCTGAATACT GTGGTGAGCT CATCTCTCAG
    GATGAGGCTG ATCGACGCGG AAAGGTCTAT GACAAATACA TGTCCAGCTT CCTCTTCAAC
    CTCAATAATG ATTTTGTAGT GGATGCTACT CGGAAAGGAA ACAAAATTCG ATTTGCAAAT
    CATTCAGTGA ATCCCAACTG TTATGCCAAA GTGGTCATGG TGAATGGAGA CCATCGGATT
    GGGATCTTTG CCAAGAGGGC AATTCAAGCT GGCGAAGAGC TCTTCTTTGA TTACAGGTAC
    AGCCAAGCTG ATGCTCTCAA GTACGTGGGG ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57642
    Clone ID Related Accession (Same CDS sequence) NM_001321081.1 , NM_001321081.2
    Accession Version NM_001321081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833159.1 and AB002386.1. On May 31, 2019 this sequence version replaced NM_001321081.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL833159.1, SRR1803617.93587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTT GTACCATAGA GAGCATTTTC
    CCGGGATTTG CAAGCCAACA TATGTTAATG AGGTCACTGA ACACAGTTGC ATTGGTTCCC
    ATCATGTATT CCTGGTCCCC TCTCCAACAG AACTTTATGG TAGAAGATGA GACGGTTTTG
    TGCAATATTC CCTACATGGG AGATGAAGTG AAAGAAGAAG ATGAGACTTT TATTGAGGAG
    CTGATCAATA ACTATGATGG GAAAGTCCAT GGTGAAGAAG AGATGATCCC TGGATCCGTT
    CTGATTAGTG ATGCTGTTTT TCTGGAGTTG GTCGATGCCC TGAATCAGTA CTCAGATGAG
    GAGGAGGAAG GGCACAATGA CACCTCAGAT GGAAAGCAGG ATGACAGCAA AGAAGATCTG
    CCAGTAACAA GAAAGAGAAA GCGACATGCT ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA
    CAGTTCCCAA ATGACATGAT CTTCAGTGCA ATTGCCTCAA TGTTCCCTGA GAATGGTGTC
    CCAGATGACA TGAAGGAGAG GTATCGAGAA CTAACAGAGA TGTCAGACCC CAATGCACTT
    CCCCCTCAGT GCACACCCAA CATCGATGGC CCCAATGCCA AGTCTGTGCA GCGGGAGCAA
    TCTCTGCACT CCTTCCACAC ACTTTTTTGC CGGCGCTGCT TTAAATACGA CTGCTTCCTT
    CACCCTTTTC ATGCCACCCC TAATGTATAT AAACGCAAGA ATAAAGAAAT CAAGATTGAA
    CCAGAACCAT GTGGCACAGA CTGCTTCCTT TTGCTGGAAG GAGCAAAGGA GTATGCCATG
    CTCCACAACC CCCGCTCCAA GTGCTCTGGT CGTCGCCGGA GAAGGCACCA CATAGTCAGT
    GCTTCCTGCT CCAATGCCTC AGCCTCTGCT GTGGCTGAGA CTAAAGAAGG AGACAGTGAC
    AGGGACACAG GCAATGACTG GGCCTCCAGT TCTTCAGAGG CTAACTCTCG CTGTCAGACT
    CCCACAAAAC AGAAGGCTAG TCCAGCCCCA CCTCAACTCT GCGTAGTGGA AGCACCCTCG
    GAGCCTGTGG AATGGACTGG GGCTGAAGAA TCTCTTTTTC GAGTCTTCCA TGGCACCTAC
    TTCAACAACT TCTGTTCAAT AGCCAGGCTT CTGGGGACCA AGACGTGCAA GCAGGTCTTT
    CAGTTTGCAG TCAAAGAATC ACTTATCCTG AAGCTGCCAA CAGATGAGCT CATGAACCCC
    TCACAGAAGA AGAAAAGAAA GCACAGATTG TGGGCTGCAC ACTGCAGGAA GATTCAGCTG
    AAGAAAGATA ACTCTTCCAC ACAAGTGTAC AACTACCAAC CCTGCGACCA CCCAGACCGC
    CCCTGTGACA GCACCTGCCC CTGCATCATG ACTCAGAATT TCTGTGAGAA GTTCTGCCAG
    TGCAACCCAG ACTGTCAGAA TCGTTTCCCT GGCTGTCGCT GTAAGACCCA GTGCAATACC
    AAGCAATGTC CTTGCTATCT GGCAGTGCGA GAATGTGACC CTGACCTGTG TCTCACCTGT
    GGGGCCTCAG AGCACTGGGA CTGCAAGGTG GTTTCCTGTA AAAACTGCAG CATCCAGCGT
    GGACTTAAGA AGCACCTGCT GCTGGCCCCC TCTGATGTGG CCGGATGGGG CACCTTCATA
    AAGGAGTCTG TGCAGAAGAA CGAATTCATT TCTGAATACT GTGGTGAGCT CATCTCTCAG
    GATGAGGCTG ATCGACGCGG AAAGGTCTAT GACAAATACA TGTCCAGCTT CCTCTTCAAC
    CTCAATAATG ATTTTGTAGT GGATGCTACT CGGAAAGGAA ACAAAATTCG ATTTGCAAAT
    CATTCAGTGA ATCCCAACTG TTATGCCAAA GTGGTCATGG TGAATGGAGA CCATCGGATT
    GGGATCTTTG CCAAGAGGGC AATTCAAGCT GGCGAAGAGC TCTTCTTTGA TTACAGGTAC
    AGCCAAGCTG ATGCTCTCAA GTACGTGGGG ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308010.1
    CDS133..2349
    Translation

    Target ORF information:

    RefSeq Version NM_001321081.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAATAC CAAATCCCCC TACCTCCAAA TGTATCACTT ACTGGAAAAG AAAAGTGAAA 
    TCTGAATACA TGCGACTTCG ACAACTTAAA CGGCTTCAGG CAAATATGGG TGCAAAGGCT
    TTGTATGTGG CAAATTTTGC AAAGGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG
    AAGAAGCTTC GTGTCCAACC TGTTCAGTCA ATGAAGCCTT GTACCATAGA GAGCATTTTC
    CCGGGATTTG CAAGCCAACA TATGTTAATG AGGTCACTGA ACACAGTTGC ATTGGTTCCC
    ATCATGTATT CCTGGTCCCC TCTCCAACAG AACTTTATGG TAGAAGATGA GACGGTTTTG
    TGCAATATTC CCTACATGGG AGATGAAGTG AAAGAAGAAG ATGAGACTTT TATTGAGGAG
    CTGATCAATA ACTATGATGG GAAAGTCCAT GGTGAAGAAG AGATGATCCC TGGATCCGTT
    CTGATTAGTG ATGCTGTTTT TCTGGAGTTG GTCGATGCCC TGAATCAGTA CTCAGATGAG
    GAGGAGGAAG GGCACAATGA CACCTCAGAT GGAAAGCAGG ATGACAGCAA AGAAGATCTG
    CCAGTAACAA GAAAGAGAAA GCGACATGCT ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA
    CAGTTCCCAA ATGACATGAT CTTCAGTGCA ATTGCCTCAA TGTTCCCTGA GAATGGTGTC
    CCAGATGACA TGAAGGAGAG GTATCGAGAA CTAACAGAGA TGTCAGACCC CAATGCACTT
    CCCCCTCAGT GCACACCCAA CATCGATGGC CCCAATGCCA AGTCTGTGCA GCGGGAGCAA
    TCTCTGCACT CCTTCCACAC ACTTTTTTGC CGGCGCTGCT TTAAATACGA CTGCTTCCTT
    CACCCTTTTC ATGCCACCCC TAATGTATAT AAACGCAAGA ATAAAGAAAT CAAGATTGAA
    CCAGAACCAT GTGGCACAGA CTGCTTCCTT TTGCTGGAAG GAGCAAAGGA GTATGCCATG
    CTCCACAACC CCCGCTCCAA GTGCTCTGGT CGTCGCCGGA GAAGGCACCA CATAGTCAGT
    GCTTCCTGCT CCAATGCCTC AGCCTCTGCT GTGGCTGAGA CTAAAGAAGG AGACAGTGAC
    AGGGACACAG GCAATGACTG GGCCTCCAGT TCTTCAGAGG CTAACTCTCG CTGTCAGACT
    CCCACAAAAC AGAAGGCTAG TCCAGCCCCA CCTCAACTCT GCGTAGTGGA AGCACCCTCG
    GAGCCTGTGG AATGGACTGG GGCTGAAGAA TCTCTTTTTC GAGTCTTCCA TGGCACCTAC
    TTCAACAACT TCTGTTCAAT AGCCAGGCTT CTGGGGACCA AGACGTGCAA GCAGGTCTTT
    CAGTTTGCAG TCAAAGAATC ACTTATCCTG AAGCTGCCAA CAGATGAGCT CATGAACCCC
    TCACAGAAGA AGAAAAGAAA GCACAGATTG TGGGCTGCAC ACTGCAGGAA GATTCAGCTG
    AAGAAAGATA ACTCTTCCAC ACAAGTGTAC AACTACCAAC CCTGCGACCA CCCAGACCGC
    CCCTGTGACA GCACCTGCCC CTGCATCATG ACTCAGAATT TCTGTGAGAA GTTCTGCCAG
    TGCAACCCAG ACTGTCAGAA TCGTTTCCCT GGCTGTCGCT GTAAGACCCA GTGCAATACC
    AAGCAATGTC CTTGCTATCT GGCAGTGCGA GAATGTGACC CTGACCTGTG TCTCACCTGT
    GGGGCCTCAG AGCACTGGGA CTGCAAGGTG GTTTCCTGTA AAAACTGCAG CATCCAGCGT
    GGACTTAAGA AGCACCTGCT GCTGGCCCCC TCTGATGTGG CCGGATGGGG CACCTTCATA
    AAGGAGTCTG TGCAGAAGAA CGAATTCATT TCTGAATACT GTGGTGAGCT CATCTCTCAG
    GATGAGGCTG ATCGACGCGG AAAGGTCTAT GACAAATACA TGTCCAGCTT CCTCTTCAAC
    CTCAATAATG ATTTTGTAGT GGATGCTACT CGGAAAGGAA ACAAAATTCG ATTTGCAAAT
    CATTCAGTGA ATCCCAACTG TTATGCCAAA GTGGTCATGG TGAATGGAGA CCATCGGATT
    GGGATCTTTG CCAAGAGGGC AATTCAAGCT GGCGAAGAGC TCTTCTTTGA TTACAGGTAC
    AGCCAAGCTG ATGCTCTCAA GTACGTGGGG ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50205
    Clone ID Related Accession (Same CDS sequence) XM_005257145.2
    Accession Version XM_005257145.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257202.1
    CDS46..2187
    Translation

    Target ORF information:

    RefSeq Version XM_005257145.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257145.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTGTG
    AGTGGACACC CTTTTCTCAA AAAGGTAGAA GATGAGACGG TTTTGTGCAA TATTCCCTAC
    ATGGGAGATG AAGTGAAAGA AGAAGATGAG ACTTTTATTG AGGAGCTGAT CAATAACTAT
    GATGGGAAAG TCCATGGTGA AGAAGAGATG ATCCCTGGAT CCGTTCTGAT TAGTGATGCT
    GTTTTTCTGG AGTTGGTCGA TGCCCTGAAT CAGTACTCAG ATGAGGAGGA GGAAGGGCAC
    AATGACACCT CAGATGGAAA GCAGGATGAC AGCAAAGAAG ATCTGCCAGT AACAAGAAAG
    AGAAAGCGAC ATGCTATTGA AGGCAACAAA AAGAGTTCCA AGAAACAGTT CCCAAATGAC
    ATGATCTTCA GTGCAATTGC CTCAATGTTC CCTGAGAATG GTGTCCCAGA TGACATGAAG
    GAGAGGTATC GAGAACTAAC AGAGATGTCA GACCCCAATG CACTTCCCCC TCAGTGCACA
    CCCAACATCG ATGGCCCCAA TGCCAAGTCT GTGCAGCGGG AGCAATCTCT GCACTCCTTC
    CACACACTTT TTTGCCGGCG CTGCTTTAAA TACGACTGCT TCCTTCACCC TTTTCATGCC
    ACCCCTAATG TATATAAACG CAAGAATAAA GAAATCAAGA TTGAACCAGA ACCATGTGGC
    ACAGACTGCT TCCTTTTGCT GGAAGGAGCA AAGGAGTATG CCATGCTCCA CAACCCCCGC
    TCCAAGTGCT CTGGTCGTCG CCGGAGAAGG CACCACATAG TCAGTGCTTC CTGCTCCAAT
    GCCTCAGCCT CTGCTGTGGC TGAGACTAAA GAAGGAGACA GTGACAGGGA CACAGGCAAT
    GACTGGGCCT CCAGTTCTTC AGAGGCTAAC TCTCGCTGTC AGACTCCCAC AAAACAGAAG
    GCTAGTCCAG CCCCACCTCA ACTCTGCGTA GTGGAAGCAC CCTCGGAGCC TGTGGAATGG
    ACTGGGGCTG AAGAATCTCT TTTTCGAGTC TTCCATGGCA CCTACTTCAA CAACTTCTGT
    TCAATAGCCA GGCTTCTGGG GACCAAGACG TGCAAGCAGG TCTTTCAGTT TGCAGTCAAA
    GAATCACTTA TCCTGAAGCT GCCAACAGAT GAGCTCATGA ACCCCTCACA GAAGAAGAAA
    AGAAAGCACA GATTGTGGGC TGCACACTGC AGGAAGATTC AGCTGAAGAA AGATAACTCT
    TCCACACAAG TGTACAACTA CCAACCCTGC GACCACCCAG ACCGCCCCTG TGACAGCACC
    TGCCCCTGCA TCATGACTCA GAATTTCTGT GAGAAGTTCT GCCAGTGCAA CCCAGACTGT
    CAGAATCGTT TCCCTGGCTG TCGCTGTAAG ACCCAGTGCA ATACCAAGCA ATGTCCTTGC
    TATCTGGCAG TGCGAGAATG TGACCCTGAC CTGTGTCTCA CCTGTGGGGC CTCAGAGCAC
    TGGGACTGCA AGGTGGTTTC CTGTAAAAAC TGCAGCATCC AGCGTGGACT TAAGAAGCAC
    CTGCTGCTGG CCCCCTCTGA TGTGGCCGGA TGGGGCACCT TCATAAAGGA GTCTGTGCAG
    AAGAACGAAT TCATTTCTGA ATACTGTGGT GAGCTCATCT CTCAGGATGA GGCTGATCGA
    CGCGGAAAGG TCTATGACAA ATACATGTCC AGCTTCCTCT TCAACCTCAA TAATGATTTT
    GTAGTGGATG CTACTCGGAA AGGAAACAAA ATTCGATTTG CAAATCATTC AGTGAATCCC
    AACTGTTATG CCAAAGTGGT CATGGTGAAT GGAGACCATC GGATTGGGAT CTTTGCCAAG
    AGGGCAATTC AAGCTGGCGA AGAGCTCTTC TTTGATTACA GGTACAGCCA AGCTGATGCT
    CTCAAGTACG TGGGGATCGA GAGGGAGACC GACGTCCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57641
    Clone ID Related Accession (Same CDS sequence) XM_011524517.2
    Accession Version XM_011524517.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTTGT
    ACCATAGAGA GCATTTTCCC GGGATTTGCA AGCCAACATA TGTTAATGAG GTCACTGAAC
    ACAGTTGCAT TGGTTCCCAT CATGTATTCC TGGTCCCCTC TCCAACAGAA CTTTATGGTA
    GAAGATGAGA CGGTTTTGTG CAATATTCCC TACATGGGAG ATGAAGTGAA AGAAGAAGAT
    GAGACTTTTA TTGAGGAGCT GATCAATAAC TATGATGGGA AAGTCCATGG TGAAGAAGAG
    ATGATCCCTG GATCCGTTCT GATTAGTGAT GCTGTTTTTC TGGAGTTGGT CGATGCCCTG
    AATCAGTACT CAGATGAGGA GGAGGAAGGG CACAATGACA CCTCAGATGG AAAGCAGGAT
    GACAGCAAAG AAGATCTGCC AGTAACAAGA AAGAGAAAGC GACATGCTAT TGAAGGCAAC
    AAAAAGAGTT CCAAGAAACA GTTCCCAAAT GACATGATCT TCAGTGCAAT TGCCTCAATG
    TTCCCTGAGA ATGGTGTCCC AGATGACATG AAGGAGAGGT ATCGAGAACT AACAGAGATG
    TCAGACCCCA ATGCACTTCC CCCTCAGTGC ACACCCAACA TCGATGGCCC CAATGCCAAG
    TCTGTGCAGC GGGAGCAATC TCTGCACTCC TTCCACACAC TTTTTTGCCG GCGCTGCTTT
    AAATACGACT GCTTCCTTCA CCCTTTTCAT GCCACCCCTA ATGTATATAA ACGCAAGAAT
    AAAGAAATCA AGATTGAACC AGAACCATGT GGCACAGACT GCTTCCTTTT GCTGGAAGGA
    GCAAAGGAGT ATGCCATGCT CCACAACCCC CGCTCCAAGT GCTCTGGTCG TCGCCGGAGA
    AGGCACCACA TAGTCAGTGC TTCCTGCTCC AATGCCTCAG CCTCTGCTGT GGCTGAGACT
    AAAGAAGGAG ACAGTGACAG GGACACAGGC AATGACTGGG CCTCCAGTTC TTCAGAGGCT
    AACTCTCGCT GTCAGACTCC CACAAAACAG AAGGCTAGTC CAGCCCCACC TCAACTCTGC
    GTAGTGGAAG CACCCTCGGA GCCTGTGGAA TGGACTGGGG CTGAAGAATC TCTTTTTCGA
    GTCTTCCATG GCACCTACTT CAACAACTTC TGTTCAATAG CCAGGCTTCT GGGGACCAAG
    ACGTGCAAGC AGGTCTTTCA GTTTGCAGTC AAAGAATCAC TTATCCTGAA GCTGCCAACA
    GATGAGCTCA TGAACCCCTC ACAGAAGAAG AAAAGAAAGC ACAGATTGTG GGCTGCACAC
    TGCAGGAAGA TTCAGCTGAA GAAAGATAAC TCTTCCACAC AAGTGTACAA CTACCAACCC
    TGCGACCACC CAGACCGCCC CTGTGACAGC ACCTGCCCCT GCATCATGAC TCAGAATTTC
    TGTGAGAAGT TCTGCCAGTG CAACCCAGAC TGTCAGAATC GTTTCCCTGG CTGTCGCTGT
    AAGACCCAGT GCAATACCAA GCAATGTCCT TGCTATCTGG CAGTGCGAGA ATGTGACCCT
    GACCTGTGTC TCACCTGTGG GGCCTCAGAG CACTGGGACT GCAAGGTGGT TTCCTGTAAA
    AACTGCAGCA TCCAGCGTGG ACTTAAGAAG CACCTGCTGC TGGCCCCCTC TGATGTGGCC
    GGATGGGGCA CCTTCATAAA GGAGTCTGTG CAGAAGAACG AATTCATTTC TGAATACTGT
    GGTGAGCTCA TCTCTCAGGA TGAGGCTGAT CGACGCGGAA AGGTCTATGA CAAATACATG
    TCCAGCTTCC TCTTCAACCT CAATAATGAT TTTGTAGTGG ATGCTACTCG GAAAGGAAAC
    AAAATTCGAT TTGCAAATCA TTCAGTGAAT CCCAACTGTT ATGCCAAAGT GGTCATGGTG
    AATGGAGACC ATCGGATTGG GATCTTTGCC AAGAGGGCAA TTCAAGCTGG CGAAGAGCTC
    TTCTTTGATT ACAGGTACAG CCAAGCTGAT GCTCTCAAGT ACGTGGGGAT CGAGAGGGAG
    ACCGACGTCC TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522819.1
    CDS46..2280
    Translation

    Target ORF information:

    RefSeq Version XM_011524517.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524517.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGGATT ACAGCAAGAT GGAAATACCA AATCCCCCTA CCTCCAAATG TATCACTTAC 
    TGGAAAAGAA AAGTGAAATC TGAATACATG CGACTTCGAC AACTTAAACG GCTTCAGGCA
    AATATGGGTG CAAAGGCTTT GTATGTGGCA AATTTTGCAA AGGTTCAAGA AAAAACCCAG
    ATCCTCAATG AAGAATGGAA GAAGCTTCGT GTCCAACCTG TTCAGTCAAT GAAGCCTTGT
    ACCATAGAGA GCATTTTCCC GGGATTTGCA AGCCAACATA TGTTAATGAG GTCACTGAAC
    ACAGTTGCAT TGGTTCCCAT CATGTATTCC TGGTCCCCTC TCCAACAGAA CTTTATGGTA
    GAAGATGAGA CGGTTTTGTG CAATATTCCC TACATGGGAG ATGAAGTGAA AGAAGAAGAT
    GAGACTTTTA TTGAGGAGCT GATCAATAAC TATGATGGGA AAGTCCATGG TGAAGAAGAG
    ATGATCCCTG GATCCGTTCT GATTAGTGAT GCTGTTTTTC TGGAGTTGGT CGATGCCCTG
    AATCAGTACT CAGATGAGGA GGAGGAAGGG CACAATGACA CCTCAGATGG AAAGCAGGAT
    GACAGCAAAG AAGATCTGCC AGTAACAAGA AAGAGAAAGC GACATGCTAT TGAAGGCAAC
    AAAAAGAGTT CCAAGAAACA GTTCCCAAAT GACATGATCT TCAGTGCAAT TGCCTCAATG
    TTCCCTGAGA ATGGTGTCCC AGATGACATG AAGGAGAGGT ATCGAGAACT AACAGAGATG
    TCAGACCCCA ATGCACTTCC CCCTCAGTGC ACACCCAACA TCGATGGCCC CAATGCCAAG
    TCTGTGCAGC GGGAGCAATC TCTGCACTCC TTCCACACAC TTTTTTGCCG GCGCTGCTTT
    AAATACGACT GCTTCCTTCA CCCTTTTCAT GCCACCCCTA ATGTATATAA ACGCAAGAAT
    AAAGAAATCA AGATTGAACC AGAACCATGT GGCACAGACT GCTTCCTTTT GCTGGAAGGA
    GCAAAGGAGT ATGCCATGCT CCACAACCCC CGCTCCAAGT GCTCTGGTCG TCGCCGGAGA
    AGGCACCACA TAGTCAGTGC TTCCTGCTCC AATGCCTCAG CCTCTGCTGT GGCTGAGACT
    AAAGAAGGAG ACAGTGACAG GGACACAGGC AATGACTGGG CCTCCAGTTC TTCAGAGGCT
    AACTCTCGCT GTCAGACTCC CACAAAACAG AAGGCTAGTC CAGCCCCACC TCAACTCTGC
    GTAGTGGAAG CACCCTCGGA GCCTGTGGAA TGGACTGGGG CTGAAGAATC TCTTTTTCGA
    GTCTTCCATG GCACCTACTT CAACAACTTC TGTTCAATAG CCAGGCTTCT GGGGACCAAG
    ACGTGCAAGC AGGTCTTTCA GTTTGCAGTC AAAGAATCAC TTATCCTGAA GCTGCCAACA
    GATGAGCTCA TGAACCCCTC ACAGAAGAAG AAAAGAAAGC ACAGATTGTG GGCTGCACAC
    TGCAGGAAGA TTCAGCTGAA GAAAGATAAC TCTTCCACAC AAGTGTACAA CTACCAACCC
    TGCGACCACC CAGACCGCCC CTGTGACAGC ACCTGCCCCT GCATCATGAC TCAGAATTTC
    TGTGAGAAGT TCTGCCAGTG CAACCCAGAC TGTCAGAATC GTTTCCCTGG CTGTCGCTGT
    AAGACCCAGT GCAATACCAA GCAATGTCCT TGCTATCTGG CAGTGCGAGA ATGTGACCCT
    GACCTGTGTC TCACCTGTGG GGCCTCAGAG CACTGGGACT GCAAGGTGGT TTCCTGTAAA
    AACTGCAGCA TCCAGCGTGG ACTTAAGAAG CACCTGCTGC TGGCCCCCTC TGATGTGGCC
    GGATGGGGCA CCTTCATAAA GGAGTCTGTG CAGAAGAACG AATTCATTTC TGAATACTGT
    GGTGAGCTCA TCTCTCAGGA TGAGGCTGAT CGACGCGGAA AGGTCTATGA CAAATACATG
    TCCAGCTTCC TCTTCAACCT CAATAATGAT TTTGTAGTGG ATGCTACTCG GAAAGGAAAC
    AAAATTCGAT TTGCAAATCA TTCAGTGAAT CCCAACTGTT ATGCCAAAGT GGTCATGGTG
    AATGGAGACC ATCGGATTGG GATCTTTGCC AAGAGGGCAA TTCAAGCTGG CGAAGAGCTC
    TTCTTTGATT ACAGGTACAG CCAAGCTGAT GCTCTCAAGT ACGTGGGGAT CGAGAGGGAG
    ACCGACGTCC TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86689
    Clone ID Related Accession (Same CDS sequence) XM_017024350.1
    Accession Version XM_017024350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGCCTT GTACCATAGA GAGCATTTTC CCGGGATTTG CAAGCCAACA TATGTTAATG 
    AGGTCACTGA ACACAGTTGC ATTGGTTCCC ATCATGTATT CCTGGTCCCC TCTCCAACAG
    AACTTTATGG TAGAAGATGA GACGGTTTTG TGCAATATTC CCTACATGGG AGATGAAGTG
    AAAGAAGAAG ATGAGACTTT TATTGAGGAG CTGATCAATA ACTATGATGG GAAAGTCCAT
    GGTGAAGAAG AGATGATCCC TGGATCCGTT CTGATTAGTG ATGCTGTTTT TCTGGAGTTG
    GTCGATGCCC TGAATCAGTA CTCAGATGAG GAGGAGGAAG GGCACAATGA CACCTCAGAT
    GGAAAGCAGG ATGACAGCAA AGAAGATCTG CCAGTAACAA GAAAGAGAAA GCGACATGCT
    ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA CAGTTCCCAA ATGACATGAT CTTCAGTGCA
    ATTGCCTCAA TGTTCCCTGA GAATGGTGTC CCAGATGACA TGAAGGAGAG GTATCGAGAA
    CTAACAGAGA TGTCAGACCC CAATGCACTT CCCCCTCAGT GCACACCCAA CATCGATGGC
    CCCAATGCCA AGTCTGTGCA GCGGGAGCAA TCTCTGCACT CCTTCCACAC ACTTTTTTGC
    CGGCGCTGCT TTAAATACGA CTGCTTCCTT CACCCTTTTC ATGCCACCCC TAATGTATAT
    AAACGCAAGA ATAAAGAAAT CAAGATTGAA CCAGAACCAT GTGGCACAGA CTGCTTCCTT
    TTGCTGGAAG GAGCAAAGGA GTATGCCATG CTCCACAACC CCCGCTCCAA GTGCTCTGGT
    CGTCGCCGGA GAAGGCACCA CATAGTCAGT GCTTCCTGCT CCAATGCCTC AGCCTCTGCT
    GTGGCTGAGA CTAAAGAAGG AGACAGTGAC AGGGACACAG GCAATGACTG GGCCTCCAGT
    TCTTCAGAGG CTAACTCTCG CTGTCAGACT CCCACAAAAC AGAAGGCTAG TCCAGCCCCA
    CCTCAACTCT GCGTAGTGGA AGCACCCTCG GAGCCTGTGG AATGGACTGG GGCTGAAGAA
    TCTCTTTTTC GAGTCTTCCA TGGCACCTAC TTCAACAACT TCTGTTCAAT AGCCAGGCTT
    CTGGGGACCA AGACGTGCAA GCAGGTCTTT CAGTTTGCAG TCAAAGAATC ACTTATCCTG
    AAGCTGCCAA CAGATGAGCT CATGAACCCC TCACAGAAGA AGAAAAGAAA GCACAGATTG
    TGGGCTGCAC ACTGCAGGAA GATTCAGCTG AAGAAAGATA ACTCTTCCAC ACAAGTGTAC
    AACTACCAAC CCTGCGACCA CCCAGACCGC CCCTGTGACA GCACCTGCCC CTGCATCATG
    ACTCAGAATT TCTGTGAGAA GTTCTGCCAG TGCAACCCAG ACTGTCAGAA TCGTTTCCCT
    GGCTGTCGCT GTAAGACCCA GTGCAATACC AAGCAATGTC CTTGCTATCT GGCAGTGCGA
    GAATGTGACC CTGACCTGTG TCTCACCTGT GGGGCCTCAG AGCACTGGGA CTGCAAGGTG
    GTTTCCTGTA AAAACTGCAG CATCCAGCGT GGACTTAAGA AGCACCTGCT GCTGGCCCCC
    TCTGATGTGG CCGGATGGGG CACCTTCATA AAGGAGTCTG TGCAGAAGAA CGAATTCATT
    TCTGAATACT GTGGTGAGCT CATCTCTCAG GATGAGGCTG ATCGACGCGG AAAGGTCTAT
    GACAAATACA TGTCCAGCTT CCTCTTCAAC CTCAATAATG ATTTTGTAGT GGATGCTACT
    CGGAAAGGAA ACAAAATTCG ATTTGCAAAT CATTCAGTGA ATCCCAACTG TTATGCCAAA
    GTGGTCATGG TGAATGGAGA CCATCGGATT GGGATCTTTG CCAAGAGGGC AATTCAAGCT
    GGCGAAGAGC TCTTCTTTGA TTACAGGTAC AGCCAAGCTG ATGCTCTCAA GTACGTGGGG
    ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879839.1
    CDS253..2259
    Translation

    Target ORF information:

    RefSeq Version XM_017024350.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGCCTT GTACCATAGA GAGCATTTTC CCGGGATTTG CAAGCCAACA TATGTTAATG 
    AGGTCACTGA ACACAGTTGC ATTGGTTCCC ATCATGTATT CCTGGTCCCC TCTCCAACAG
    AACTTTATGG TAGAAGATGA GACGGTTTTG TGCAATATTC CCTACATGGG AGATGAAGTG
    AAAGAAGAAG ATGAGACTTT TATTGAGGAG CTGATCAATA ACTATGATGG GAAAGTCCAT
    GGTGAAGAAG AGATGATCCC TGGATCCGTT CTGATTAGTG ATGCTGTTTT TCTGGAGTTG
    GTCGATGCCC TGAATCAGTA CTCAGATGAG GAGGAGGAAG GGCACAATGA CACCTCAGAT
    GGAAAGCAGG ATGACAGCAA AGAAGATCTG CCAGTAACAA GAAAGAGAAA GCGACATGCT
    ATTGAAGGCA ACAAAAAGAG TTCCAAGAAA CAGTTCCCAA ATGACATGAT CTTCAGTGCA
    ATTGCCTCAA TGTTCCCTGA GAATGGTGTC CCAGATGACA TGAAGGAGAG GTATCGAGAA
    CTAACAGAGA TGTCAGACCC CAATGCACTT CCCCCTCAGT GCACACCCAA CATCGATGGC
    CCCAATGCCA AGTCTGTGCA GCGGGAGCAA TCTCTGCACT CCTTCCACAC ACTTTTTTGC
    CGGCGCTGCT TTAAATACGA CTGCTTCCTT CACCCTTTTC ATGCCACCCC TAATGTATAT
    AAACGCAAGA ATAAAGAAAT CAAGATTGAA CCAGAACCAT GTGGCACAGA CTGCTTCCTT
    TTGCTGGAAG GAGCAAAGGA GTATGCCATG CTCCACAACC CCCGCTCCAA GTGCTCTGGT
    CGTCGCCGGA GAAGGCACCA CATAGTCAGT GCTTCCTGCT CCAATGCCTC AGCCTCTGCT
    GTGGCTGAGA CTAAAGAAGG AGACAGTGAC AGGGACACAG GCAATGACTG GGCCTCCAGT
    TCTTCAGAGG CTAACTCTCG CTGTCAGACT CCCACAAAAC AGAAGGCTAG TCCAGCCCCA
    CCTCAACTCT GCGTAGTGGA AGCACCCTCG GAGCCTGTGG AATGGACTGG GGCTGAAGAA
    TCTCTTTTTC GAGTCTTCCA TGGCACCTAC TTCAACAACT TCTGTTCAAT AGCCAGGCTT
    CTGGGGACCA AGACGTGCAA GCAGGTCTTT CAGTTTGCAG TCAAAGAATC ACTTATCCTG
    AAGCTGCCAA CAGATGAGCT CATGAACCCC TCACAGAAGA AGAAAAGAAA GCACAGATTG
    TGGGCTGCAC ACTGCAGGAA GATTCAGCTG AAGAAAGATA ACTCTTCCAC ACAAGTGTAC
    AACTACCAAC CCTGCGACCA CCCAGACCGC CCCTGTGACA GCACCTGCCC CTGCATCATG
    ACTCAGAATT TCTGTGAGAA GTTCTGCCAG TGCAACCCAG ACTGTCAGAA TCGTTTCCCT
    GGCTGTCGCT GTAAGACCCA GTGCAATACC AAGCAATGTC CTTGCTATCT GGCAGTGCGA
    GAATGTGACC CTGACCTGTG TCTCACCTGT GGGGCCTCAG AGCACTGGGA CTGCAAGGTG
    GTTTCCTGTA AAAACTGCAG CATCCAGCGT GGACTTAAGA AGCACCTGCT GCTGGCCCCC
    TCTGATGTGG CCGGATGGGG CACCTTCATA AAGGAGTCTG TGCAGAAGAA CGAATTCATT
    TCTGAATACT GTGGTGAGCT CATCTCTCAG GATGAGGCTG ATCGACGCGG AAAGGTCTAT
    GACAAATACA TGTCCAGCTT CCTCTTCAAC CTCAATAATG ATTTTGTAGT GGATGCTACT
    CGGAAAGGAA ACAAAATTCG ATTTGCAAAT CATTCAGTGA ATCCCAACTG TTATGCCAAA
    GTGGTCATGG TGAATGGAGA CCATCGGATT GGGATCTTTG CCAAGAGGGC AATTCAAGCT
    GGCGAAGAGC TCTTCTTTGA TTACAGGTAC AGCCAAGCTG ATGCTCTCAA GTACGTGGGG
    ATCGAGAGGG AGACCGACGT CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.