EVPL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EVPL
Entrez Gene ID	2125
Full Name	envoplakin
Synonyms	EVPK
General protein information	Preferred Names envoplakin Names envoplakin 210 kDa cornified envelope precursor protein 210 kDa paraneoplastic pemphigus antigen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Summary	This gene encodes a member of the plakin family of proteins that forms a component of desmosomes and the epidermal cornified envelope. This gene is located in the tylosis oesophageal cancer locus on chromosome 17q25, and its deletion is associated with both familial and sporadic forms of oesophageal squamous cell carcinoma. Patients suffering from the autoimmune mucocutaneous disorder, paraneoplastic pemphigus, develop antibodies against the encoded protein. [provided by RefSeq, Jul 2016].
Disorder MIM:	601590

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320747.1	NP_001307676.1	envoplakin isoform 1
NM_001988.4	NP_001979.2	envoplakin isoform 2
NM_001988.2	NP_001979.2	envoplakin
NM_001988.3	NP_001979.2	envoplakin isoform 2
NM_001320747.2	NP_001307676.1	envoplakin isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EVPL	XP_003315788.1
	EVPL	XP_001101955.2
Canis lupus familiaris (dog)	EVPL	XP_540443.2
Mus musculus (house mouse)	Evpl	NP_079552.3
Xenopus tropicalis (tropical clawed frog)	evpl	NP_001128291.1
Homo sapiens (human)	EVPL	NP_001979.2
Gallus gallus (chicken)	EVPL	XP_004946182.1
Danio rerio (zebrafish)	evplb	XP_001922073.4
Bos taurus (cattle)	EVPL	NP_001192605.1
Rattus norvegicus (Norway rat)	Evpl	NP_001100536.1
Danio rerio (zebrafish)	evpla	NP_001112369.1

Gene Ontology
Bibliography

Related articles in PubMed

Extremities of the N-terminus of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus.
Wang X, Chen T, Zhao J, Peng Y, Chen X, Tu P, Zhu X, Liu Z, Wang M
Journal of dermatological science84(1)24-29(2016 Oct)

Selected single-nucleotide polymorphisms in FOXE1, SERPINA5, FTO, EVPL, TICAM1 and SCARB1 are associated with papillary and follicular thyroid cancer risk: replication study in a German population.
Sigurdson AJ, Brenner AV, Roach JA, Goudeva L, M?ller JA, Nerlich K, Reiners C, Schwab R, Pfeiffer L, Waldenberger M, Braganza M, Xu L, Sturgis EM, Yeager M, Chanock SJ, Pfeiffer RM, Abend M, Port M
Carcinogenesis37(7)677-684(2016 Jul)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Martins-de-Souza D, Guest PC, Mann DM, Roeber S, Rahmoune H, Bauder C, Kretzschmar H, Volk B, Baborie A, Bahn S
Journal of proteome research11(4)2533-43(2012 Apr)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N-terminus extremities of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus
Title: Extremities of the N-terminus of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus.

We confirmed associations with papillary thyroid cancer and SNPs in FOXE1/HEMGN, SERPINA5 (rs2069974), FTO (rs8047395), EVPL (rs2071194), TICAM1 (rs8120) and SCARB1 (rs11057820) genes. We found associations with SNPs in FOXE1, SERPINA5, FTO, TICAM1 and HSPA6 and and follicular thyroid cancer
Title: Selected single-nucleotide polymorphisms in FOXE1, SERPINA5, FTO, EVPL, TICAM1 and SCARB1 are associated with papillary and follicular thyroid cancer risk: replication study in a German population.

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

autoantibodies are detected in patients with paraneoplastic pemphigus
Title: Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus.

EVPL might not be the target gene responsible for oesophageal cancer, despite its strong candidacy in terms of character and localization
Title: Infrequent mutation of the human envoplakin gene is closely linked to the tylosis oesophageal cancer locus in sporadic oesophageal squamous cell carcinomas.

The following EVPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EVPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu57611	NM_001320747.2 Latest version!	Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1091.30 $1559.00
OHu08934	NM_001988.4 Latest version!	Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00
View Old Accession Versions >>
OHu08934	NM_001988.2	Homo sapiens envoplakin (EVPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00
OHu08934	NM_001988.3	Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00
OHu57611	NM_001320747.1	Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EVPL ( NM_001320747.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307676.1 Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu57611
Clone ID Related Accession (Same CDS sequence)	NM_001320747.1 , NM_001320747.2
Accession Version	NM_001320747.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	envoplakin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA449929.1, BC143814.1, U53786.1 and BX114092.1. This sequence is a reference standard in the RefSeqGene project. On Mar 4, 2016 this sequence version replaced XM_011524516.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143814.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGGTA CCCAGGGTGG CCCGGGGAGA 
GGTGGCTGTG GGGCTAGAAG GAGTAGATTC CGACTTCCGA CTGCTCCATC TGGCTCAGAC 
CTGGCCAACC CACAGGCCCA GAAGCTCCTG ACACAGATGA CCCGGCTGGA TGGAGACCTG 
GGACAGATAG AGAGGCAGGT GCTGGCCTGG GCGCGGGCCC CGCTGAGCCG CCCCACACCC 
TTGGAGGACT TGGAGGGCCG CATCCACAGC CATGAGGGCA CAGCCCAGCG CCTGCAGAGC 
CTGGGAACGG AGAAGGAGAC AGCCCAGAAG GAGTGCGAGG CGTTTCTGTC CACGCGGCCC 
GTGGGCCCCG CTGCCCTGCA GCTGCCCGTA GCCCTCAACA GCGTGAAGAA CAAGTTCAGT 
GACGTGCAGG TTCTGTGCAG CCTCTACGGG GAGAAAGCCA AGGCTGCCCT GGATCTGGAG 
CGGCAGATCC AGGATGCGGA CAGGGTCATC CGAGGCTTCG AGGCCACCCT GGTGCAGGAG 
GCCCCCATCC CTGCTGAACC GGGGGCTCTG CAGGAGAGGG TCAGCGAGCT GCAGCGCCAG 
CGGAGGGAGC TGCTGGAACA GCAGACCTGC GTGCTGCGGC TACACCGCGC GCTGAAGGCC 
TCGGAGCACG CATGCGCTGC CCTGCAGAAC AACTTCCAGG AGTTCTGCCA AGACCTGCCT 
CGCCAGCAGC GCCAGGTGCG AGCCCTCACC GACCGCTACC ACGCCGTAGG GGACCAGCTG 
GACCTGCGGG AGAAGGTGGT GCAGGATGCC GCCCTCACCT ACCAGCAGTT CAAGAACTGC 
AAGGATAACC TGAGCTCCTG GCTGGAGCAC CTGCCCCGCA GCCAGGTGCG GCCCAGCGAC 
GGCCCCAGCC AGATCGCCTA CAAGCTGCAG GCGCAGAAGA GGCTGACGCA GGAGATCCAG 
AGCCGAGAGC GGGACAGGGC CACAGCATCC CACCTCTCCC AGGCCCTGCA GGCAGCGCTC 
CAGGACTATG AGCTCCAGGC AGACACCTAC CGCTGCTCTT TGGAGCCCAC CCTGGCAGTG 
TCAGCCCCCA AGAGACCCCG AGTGGCTCCC CTGCAAGAGA GCATCCAAGC CCAGGAGAAG 
AACCTTGCAA AGGCCTATAC TGAGGTTGCA GCAGCACAGC AGCAGCTGCT CCAGCAGCTG 
GAGTTTGCTA GAAAAATGCT GGAGAAGAAG GAGCTCAGTG AGGACATCCG AAGGACCCAT 
GATGCAAAGC AGGGCTCCGA GAGCCCTGCC CAAGCAGGGA GAGAGTCAGA GGCCCTGAAG 
GCCCAGCTGG AAGAGGAGAG GAAGCGGGTG GCCCGGGTGC AGCATGAGCT GGAGGCGCAG 
AGGAGCCAAC TGCTGCAGCT GAGGACCCAG CGGCCCTTGG AGAGGCTGGA GGAGAAGGAA 
GTGGTAGAGT TCTACCGGGA CCCCCAGCTG GAGGGCAGCC TGTCCAGGGT GAAGGCCCAG 
GTGGAGGAGG AGGGCAAGCG GCGGGCTGGC CTGCAGGCAG ACCTGGAAGT GGCAGCCCAG 
AAGGTCGTGC AGCTGGAAAG CAAGAGGAAG ACCATGCAGC CTCATCTGCT GACCAAGGAG 
GTCACCCAGG TGGAGAGGGA CCCCGGCCTG GACAGCCAGG CGGCCCAGCT CAGGATCCAG 
ATCCAGCAGC TCCGCGGGGA GGATGCCGTC ATCTCGGCCC GGCTGGAAGG GCTGAAGAAG 
GAGCTACTGG CCCTTGAGAA GAGGGAGGTG GACGTGAAGG AGAAGGTCGT GGTGAAAGAG 
GTAGTCAAGG TGGAGAAGAA TCTGGAAATG GTCAAGGCAG CCCAGGCTCT GAGGCTGCAG 
ATGGAGGAGG ATGCTGCGCG GAGGAAGCAG GCGGAGGAGG CTGTGGCCAA GCTACAGGCT 
CGCATCGAAG ACCTGGAGCG GGCTATCAGC TCGGTGGAGC CCAAGGTCAT CGTGAAGGAG 
GTGAAGAAGG TGGAGCAGGA CCCAGGGCTC CTCCAGGAGT CCTCCAGGCT GAGGAGCCTC 
CTCGAGGAGG AGAGGACCAA GAACGCGACG CTGGCCAGGG AGCTGAGCGA CCTGCACAGC 
AAGTACAGCG TGGTGGAGAA GCAGAGGCCC AAAGTGCAGC TCCAGGAGCG CGTCCACGAG 
ATCTTCCAGG TGGATCCGGA GACAGAGCAG GAGATCACTC GGCTCAAGGC CAAGCTGCAG 
GAGATGGCGG GCAAGAGGAG CGGTGTGGAG AAGGAGGTGG AGAAGCTGCT GCCCGACCTG 
GAGGTCCTGC GGGCCCAGAA GCCCACGGTG GAGTACAAGG AGGTGACCCA GGAGGTGGTG 
AGGCATGAGA GGAGCCCCGA GGTGCTGCGT GAGATCGACC GCCTGAAGGC TCAGCTCAAC 
GAGCTCGTCA ACAGCCACGG GCGCTCCCAG GAGCAGCTCA TCCGCCTGCA GGGTGAGCGC 
GACGAGTGGA GGCGCGAGCG GGCCAAGGTG GAGACCAAGA CGGTGAGCAA GGAGGTGGTG 
CGCCACGAGA AGGACCCGGT GCTGGAGAAA GAAGCAGAGC GGCTCCGCCA GGAGGTGCGG 
GAGGCGGCCC AGAAGAGGCG GGCCGCGGAG GACGCGGTGT ACGAGCTGCA GAGCAAGCGC 
CTGCTGCTGG AGAGGAGGAA GCCCGAGGAG AAGGTGGTGG TGCAGGAGGT GGTGGTCACC 
CAGAAGGACC CGAAGCTGCG CGAGGAGCAC AGCCGGCTGA GCGGGAGCCT GGATGAGGAG 
GTGGGCCGGC GGCGCCAGCT AGAGCTTGAG GTGCAGCAGC TGCGGGCCGG CGTGGAGGAG 
CAGGAGGGCC TGCTCAGCTT CCAGGAGGAC CGCAGCAAGA AGCTGGCCGT GGAGAGGGAG 
CTGCGGCAGC TGACCTTGAG GATCCAGGAG CTCGAGAAGC GGCCTCCCAC GGTGCAGGAG 
AAGATCATCA TGGAGGAAGT GGTCAAGCTG GAGAAGGACC CGGACCTGGA GAAGTCCACG 
GAAGCCCTGC GGTGGGACCT GGACCAGGAG AAGACCCAGG TAACCGAGCT GAATCGGGAG 
TGCAAGAACC TGCAGGTCCA GATTGACGTC CTCCAGAAAG CCAAATCGCA GGAGAAGACC 
ATCTACAAGG AAGTGATCCG GGTGCAGAAG GACCGCGTCC TGGAAGATGA GCGGGCCCGC 
GTGTGGGAGA TGCTCAACAG GGAGCGCACG GCCCGGCAGG CCCGGGAGGA GGAGGCACGG 
CGCCTGCGGG AGCGCATTGA CCGGGCCGAG ACGCTGGGGA GAACCTGGTC CCGGGAGGAG 
TCCGAGCTGC AGAGGGCCCG GGACCAGGCC GACCAGGAGT GTGGGCGGCT GCAGCAGGAG 
CTGCGGGCTC TGGAGAGGCA GAAGCAGCAG CAGACACTGC AGCTGCAGGA GGAGTCGAAG 
CTGCTCAGCC AGAAGACGGA GAGCGAGCGA CAGAAGGCGG CCCAGCGGGG CCAGGAGCTC 
TCGCGGCTGG AGGCGGCCAT CCTCCGCGAG AAGGACCAGA TCTACGAGAA GGAGCGGACG 
CTCCGGGACC TCCACGCCAA GGTGAGCCGG GAGGAGCTCA GCCAGGAGAC CCAGACGCGA 
GAGACCAACC TTTCCACCAA GATCTCCATC CTGGAACCCG AGACGGGGAA GGACATGTCC 
CCATACGAGG CCTACAAGAG GGGCATCATC GACAGGGGCC AGTACTTGCA GCTGCAGGAG 
CTCGAGTGTG ACTGGGAGGA GGTCACCACC TCGGGGCCCT GTGGGGAGGA GTCTGTGCTC 
CTGGACCGCA AGAGCGGGAA GCAGTACTCC ATCGAGGCCG CCCTCCGCTG CCGGCGCATC 
TCTAAGGAGG AGTACCATCT GTACAAGGAC GGCCACCTGC CCATCTCCGA GTTTGCGCTG 
CTTGTAGCTG GGGAGACCAA GCCAAGCTCC TCACTCTCCA TCGGCTCTAT CATCTCCAAG 
TCCCCGCTCG CCTCCCCGGC CCCCCAGAGC ACCAGTTTCT TCTCTCCCAG CTTCTCTCTC 
GGGCTCGGTG ATGACAGCTT CCCTATCGCC GGGATCTATG ACACAACCAC AGACAACAAG 
TGCAGCATCA AGACGGCCGT GGCCAAGAAC ATGCTGGACC CCATCACTGG GCAGAAGCTA 
CTGGAGGCCC AGGCGGCCAC AGGGGGCATC GTGGACCTGC TCAGCCGTGA GCGCTACTCT 
GTGCACAAGG CGATGGAGAG GGGCCTGATC GAGAACACCT CCACACAGAG GCTGCTTAAC 
GCCCAGAAGG CCTTCACCGG CATCGAGGAC CCCGTCACCA AGAAGAGGCT CTCGGTGGGC 
GAGGCCGTCC AGAAGGGCTG GATGCCCCGG GAGAGCGTGC TCCCACACCT GCAGGTGCAG 
CACCTGACCG GGGGGCTCAT CGACCCCAAG AGGACAGGCC GCATCCCCAT CCAGCAGGCC 
CTCCTCTCCG GGATGATCAG TGAAGAGCTG GCCCAGCTCC TGCAGGACGA GTCCAGCTAC 
GAGAAGGATT TGACAGACCC CATCTCCAAG GAACGGCTGA GCTACAAGGA GGCCATGGGC 
CGCTGCCGCA AAGACCCCCT GAGCGGCCTG CTGCTCCTGC CAGCGGCACT GGAGGGGTAC 
CGCTGCTACC GCTCCGCCTC CCCCACCGTC CCGCGCTCCC TTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307676.1
CDS	255..6422
Translation	MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQGTQGGPGRGGCGARRSRFRLPTAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Target ORF information:

RefSeq Version	NM_001320747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320747.1

ORF Insert Sequence:

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGGTA CCCAGGGTGG CCCGGGGAGA 
GGTGGCTGTG GGGCTAGAAG GAGTAGATTC CGACTTCCGA CTGCTCCATC TGGCTCAGAC 
CTGGCCAACC CACAGGCCCA GAAGCTCCTG ACACAGATGA CCCGGCTGGA TGGAGACCTG 
GGACAGATAG AGAGGCAGGT GCTGGCCTGG GCGCGGGCCC CGCTGAGCCG CCCCACACCC 
TTGGAGGACT TGGAGGGCCG CATCCACAGC CATGAGGGCA CAGCCCAGCG CCTGCAGAGC 
CTGGGAACGG AGAAGGAGAC AGCCCAGAAG GAGTGCGAGG CGTTTCTGTC CACGCGGCCC 
GTGGGCCCCG CTGCCCTGCA GCTGCCCGTA GCCCTCAACA GCGTGAAGAA CAAGTTCAGT 
GACGTGCAGG TTCTGTGCAG CCTCTACGGG GAGAAAGCCA AGGCTGCCCT GGATCTGGAG 
CGGCAGATCC AGGATGCGGA CAGGGTCATC CGAGGCTTCG AGGCCACCCT GGTGCAGGAG 
GCCCCCATCC CTGCTGAACC GGGGGCTCTG CAGGAGAGGG TCAGCGAGCT GCAGCGCCAG 
CGGAGGGAGC TGCTGGAACA GCAGACCTGC GTGCTGCGGC TACACCGCGC GCTGAAGGCC 
TCGGAGCACG CATGCGCTGC CCTGCAGAAC AACTTCCAGG AGTTCTGCCA AGACCTGCCT 
CGCCAGCAGC GCCAGGTGCG AGCCCTCACC GACCGCTACC ACGCCGTAGG GGACCAGCTG 
GACCTGCGGG AGAAGGTGGT GCAGGATGCC GCCCTCACCT ACCAGCAGTT CAAGAACTGC 
AAGGATAACC TGAGCTCCTG GCTGGAGCAC CTGCCCCGCA GCCAGGTGCG GCCCAGCGAC 
GGCCCCAGCC AGATCGCCTA CAAGCTGCAG GCGCAGAAGA GGCTGACGCA GGAGATCCAG 
AGCCGAGAGC GGGACAGGGC CACAGCATCC CACCTCTCCC AGGCCCTGCA GGCAGCGCTC 
CAGGACTATG AGCTCCAGGC AGACACCTAC CGCTGCTCTT TGGAGCCCAC CCTGGCAGTG 
TCAGCCCCCA AGAGACCCCG AGTGGCTCCC CTGCAAGAGA GCATCCAAGC CCAGGAGAAG 
AACCTTGCAA AGGCCTATAC TGAGGTTGCA GCAGCACAGC AGCAGCTGCT CCAGCAGCTG 
GAGTTTGCTA GAAAAATGCT GGAGAAGAAG GAGCTCAGTG AGGACATCCG AAGGACCCAT 
GATGCAAAGC AGGGCTCCGA GAGCCCTGCC CAAGCAGGGA GAGAGTCAGA GGCCCTGAAG 
GCCCAGCTGG AAGAGGAGAG GAAGCGGGTG GCCCGGGTGC AGCATGAGCT GGAGGCGCAG 
AGGAGCCAAC TGCTGCAGCT GAGGACCCAG CGGCCCTTGG AGAGGCTGGA GGAGAAGGAA 
GTGGTAGAGT TCTACCGGGA CCCCCAGCTG GAGGGCAGCC TGTCCAGGGT GAAGGCCCAG 
GTGGAGGAGG AGGGCAAGCG GCGGGCTGGC CTGCAGGCAG ACCTGGAAGT GGCAGCCCAG 
AAGGTCGTGC AGCTGGAAAG CAAGAGGAAG ACCATGCAGC CTCATCTGCT GACCAAGGAG 
GTCACCCAGG TGGAGAGGGA CCCCGGCCTG GACAGCCAGG CGGCCCAGCT CAGGATCCAG 
ATCCAGCAGC TCCGCGGGGA GGATGCCGTC ATCTCGGCCC GGCTGGAAGG GCTGAAGAAG 
GAGCTACTGG CCCTTGAGAA GAGGGAGGTG GACGTGAAGG AGAAGGTCGT GGTGAAAGAG 
GTAGTCAAGG TGGAGAAGAA TCTGGAAATG GTCAAGGCAG CCCAGGCTCT GAGGCTGCAG 
ATGGAGGAGG ATGCTGCGCG GAGGAAGCAG GCGGAGGAGG CTGTGGCCAA GCTACAGGCT 
CGCATCGAAG ACCTGGAGCG GGCTATCAGC TCGGTGGAGC CCAAGGTCAT CGTGAAGGAG 
GTGAAGAAGG TGGAGCAGGA CCCAGGGCTC CTCCAGGAGT CCTCCAGGCT GAGGAGCCTC 
CTCGAGGAGG AGAGGACCAA GAACGCGACG CTGGCCAGGG AGCTGAGCGA CCTGCACAGC 
AAGTACAGCG TGGTGGAGAA GCAGAGGCCC AAAGTGCAGC TCCAGGAGCG CGTCCACGAG 
ATCTTCCAGG TGGATCCGGA GACAGAGCAG GAGATCACTC GGCTCAAGGC CAAGCTGCAG 
GAGATGGCGG GCAAGAGGAG CGGTGTGGAG AAGGAGGTGG AGAAGCTGCT GCCCGACCTG 
GAGGTCCTGC GGGCCCAGAA GCCCACGGTG GAGTACAAGG AGGTGACCCA GGAGGTGGTG 
AGGCATGAGA GGAGCCCCGA GGTGCTGCGT GAGATCGACC GCCTGAAGGC TCAGCTCAAC 
GAGCTCGTCA ACAGCCACGG GCGCTCCCAG GAGCAGCTCA TCCGCCTGCA GGGTGAGCGC 
GACGAGTGGA GGCGCGAGCG GGCCAAGGTG GAGACCAAGA CGGTGAGCAA GGAGGTGGTG 
CGCCACGAGA AGGACCCGGT GCTGGAGAAA GAAGCAGAGC GGCTCCGCCA GGAGGTGCGG 
GAGGCGGCCC AGAAGAGGCG GGCCGCGGAG GACGCGGTGT ACGAGCTGCA GAGCAAGCGC 
CTGCTGCTGG AGAGGAGGAA GCCCGAGGAG AAGGTGGTGG TGCAGGAGGT GGTGGTCACC 
CAGAAGGACC CGAAGCTGCG CGAGGAGCAC AGCCGGCTGA GCGGGAGCCT GGATGAGGAG 
GTGGGCCGGC GGCGCCAGCT AGAGCTTGAG GTGCAGCAGC TGCGGGCCGG CGTGGAGGAG 
CAGGAGGGCC TGCTCAGCTT CCAGGAGGAC CGCAGCAAGA AGCTGGCCGT GGAGAGGGAG 
CTGCGGCAGC TGACCTTGAG GATCCAGGAG CTCGAGAAGC GGCCTCCCAC GGTGCAGGAG 
AAGATCATCA TGGAGGAAGT GGTCAAGCTG GAGAAGGACC CGGACCTGGA GAAGTCCACG 
GAAGCCCTGC GGTGGGACCT GGACCAGGAG AAGACCCAGG TAACCGAGCT GAATCGGGAG 
TGCAAGAACC TGCAGGTCCA GATTGACGTC CTCCAGAAAG CCAAATCGCA GGAGAAGACC 
ATCTACAAGG AAGTGATCCG GGTGCAGAAG GACCGCGTCC TGGAAGATGA GCGGGCCCGC 
GTGTGGGAGA TGCTCAACAG GGAGCGCACG GCCCGGCAGG CCCGGGAGGA GGAGGCACGG 
CGCCTGCGGG AGCGCATTGA CCGGGCCGAG ACGCTGGGGA GAACCTGGTC CCGGGAGGAG 
TCCGAGCTGC AGAGGGCCCG GGACCAGGCC GACCAGGAGT GTGGGCGGCT GCAGCAGGAG 
CTGCGGGCTC TGGAGAGGCA GAAGCAGCAG CAGACACTGC AGCTGCAGGA GGAGTCGAAG 
CTGCTCAGCC AGAAGACGGA GAGCGAGCGA CAGAAGGCGG CCCAGCGGGG CCAGGAGCTC 
TCGCGGCTGG AGGCGGCCAT CCTCCGCGAG AAGGACCAGA TCTACGAGAA GGAGCGGACG 
CTCCGGGACC TCCACGCCAA GGTGAGCCGG GAGGAGCTCA GCCAGGAGAC CCAGACGCGA 
GAGACCAACC TTTCCACCAA GATCTCCATC CTGGAACCCG AGACGGGGAA GGACATGTCC 
CCATACGAGG CCTACAAGAG GGGCATCATC GACAGGGGCC AGTACTTGCA GCTGCAGGAG 
CTCGAGTGTG ACTGGGAGGA GGTCACCACC TCGGGGCCCT GTGGGGAGGA GTCTGTGCTC 
CTGGACCGCA AGAGCGGGAA GCAGTACTCC ATCGAGGCCG CCCTCCGCTG CCGGCGCATC 
TCTAAGGAGG AGTACCATCT GTACAAGGAC GGCCACCTGC CCATCTCCGA GTTTGCGCTG 
CTTGTAGCTG GGGAGACCAA GCCAAGCTCC TCACTCTCCA TCGGCTCTAT CATCTCCAAG 
TCCCCGCTCG CCTCCCCGGC CCCCCAGAGC ACCAGTTTCT TCTCTCCCAG CTTCTCTCTC 
GGGCTCGGTG ATGACAGCTT CCCTATCGCC GGGATCTATG ACACAACCAC AGACAACAAG 
TGCAGCATCA AGACGGCCGT GGCCAAGAAC ATGCTGGACC CCATCACTGG GCAGAAGCTA 
CTGGAGGCCC AGGCGGCCAC AGGGGGCATC GTGGACCTGC TCAGCCGTGA GCGCTACTCT 
GTGCACAAGG CGATGGAGAG GGGCCTGATC GAGAACACCT CCACACAGAG GCTGCTTAAC 
GCCCAGAAGG CCTTCACCGG CATCGAGGAC CCCGTCACCA AGAAGAGGCT CTCGGTGGGC 
GAGGCCGTCC AGAAGGGCTG GATGCCCCGG GAGAGCGTGC TCCCACACCT GCAGGTGCAG 
CACCTGACCG GGGGGCTCAT CGACCCCAAG AGGACAGGCC GCATCCCCAT CCAGCAGGCC 
CTCCTCTCCG GGATGATCAG TGAAGAGCTG GCCCAGCTCC TGCAGGACGA GTCCAGCTAC 
GAGAAGGATT TGACAGACCC CATCTCCAAG GAACGGCTGA GCTACAAGGA GGCCATGGGC 
CGCTGCCGCA AAGACCCCCT GAGCGGCCTG CTGCTCCTGC CAGCGGCACT GGAGGGGTAC 
CGCTGCTACC GCTCCGCCTC CCCCACCGTC CCGCGCTCCC TTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVPL ( NM_001320747.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307676.1 Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.

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CloneID	OHu57611
Clone ID Related Accession (Same CDS sequence)	NM_001320747.1 , NM_001320747.2
Accession Version	NM_001320747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	envoplakin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA449929.1, BC143814.1, U53786.1 and BX114092.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001320747.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143814.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGGTA CCCAGGGTGG CCCGGGGAGA 
GGTGGCTGTG GGGCTAGAAG GAGTAGATTC CGACTTCCGA CTGCTCCATC TGGCTCAGAC 
CTGGCCAACC CACAGGCCCA GAAGCTCCTG ACACAGATGA CCCGGCTGGA TGGAGACCTG 
GGACAGATAG AGAGGCAGGT GCTGGCCTGG GCGCGGGCCC CGCTGAGCCG CCCCACACCC 
TTGGAGGACT TGGAGGGCCG CATCCACAGC CATGAGGGCA CAGCCCAGCG CCTGCAGAGC 
CTGGGAACGG AGAAGGAGAC AGCCCAGAAG GAGTGCGAGG CGTTTCTGTC CACGCGGCCC 
GTGGGCCCCG CTGCCCTGCA GCTGCCCGTA GCCCTCAACA GCGTGAAGAA CAAGTTCAGT 
GACGTGCAGG TTCTGTGCAG CCTCTACGGG GAGAAAGCCA AGGCTGCCCT GGATCTGGAG 
CGGCAGATCC AGGATGCGGA CAGGGTCATC CGAGGCTTCG AGGCCACCCT GGTGCAGGAG 
GCCCCCATCC CTGCTGAACC GGGGGCTCTG CAGGAGAGGG TCAGCGAGCT GCAGCGCCAG 
CGGAGGGAGC TGCTGGAACA GCAGACCTGC GTGCTGCGGC TACACCGCGC GCTGAAGGCC 
TCGGAGCACG CATGCGCTGC CCTGCAGAAC AACTTCCAGG AGTTCTGCCA AGACCTGCCT 
CGCCAGCAGC GCCAGGTGCG AGCCCTCACC GACCGCTACC ACGCCGTAGG GGACCAGCTG 
GACCTGCGGG AGAAGGTGGT GCAGGATGCC GCCCTCACCT ACCAGCAGTT CAAGAACTGC 
AAGGATAACC TGAGCTCCTG GCTGGAGCAC CTGCCCCGCA GCCAGGTGCG GCCCAGCGAC 
GGCCCCAGCC AGATCGCCTA CAAGCTGCAG GCGCAGAAGA GGCTGACGCA GGAGATCCAG 
AGCCGAGAGC GGGACAGGGC CACAGCATCC CACCTCTCCC AGGCCCTGCA GGCAGCGCTC 
CAGGACTATG AGCTCCAGGC AGACACCTAC CGCTGCTCTT TGGAGCCCAC CCTGGCAGTG 
TCAGCCCCCA AGAGACCCCG AGTGGCTCCC CTGCAAGAGA GCATCCAAGC CCAGGAGAAG 
AACCTTGCAA AGGCCTATAC TGAGGTTGCA GCAGCACAGC AGCAGCTGCT CCAGCAGCTG 
GAGTTTGCTA GAAAAATGCT GGAGAAGAAG GAGCTCAGTG AGGACATCCG AAGGACCCAT 
GATGCAAAGC AGGGCTCCGA GAGCCCTGCC CAAGCAGGGA GAGAGTCAGA GGCCCTGAAG 
GCCCAGCTGG AAGAGGAGAG GAAGCGGGTG GCCCGGGTGC AGCATGAGCT GGAGGCGCAG 
AGGAGCCAAC TGCTGCAGCT GAGGACCCAG CGGCCCTTGG AGAGGCTGGA GGAGAAGGAA 
GTGGTAGAGT TCTACCGGGA CCCCCAGCTG GAGGGCAGCC TGTCCAGGGT GAAGGCCCAG 
GTGGAGGAGG AGGGCAAGCG GCGGGCTGGC CTGCAGGCAG ACCTGGAAGT GGCAGCCCAG 
AAGGTCGTGC AGCTGGAAAG CAAGAGGAAG ACCATGCAGC CTCATCTGCT GACCAAGGAG 
GTCACCCAGG TGGAGAGGGA CCCCGGCCTG GACAGCCAGG CGGCCCAGCT CAGGATCCAG 
ATCCAGCAGC TCCGCGGGGA GGATGCCGTC ATCTCGGCCC GGCTGGAAGG GCTGAAGAAG 
GAGCTACTGG CCCTTGAGAA GAGGGAGGTG GACGTGAAGG AGAAGGTCGT GGTGAAAGAG 
GTAGTCAAGG TGGAGAAGAA TCTGGAAATG GTCAAGGCAG CCCAGGCTCT GAGGCTGCAG 
ATGGAGGAGG ATGCTGCGCG GAGGAAGCAG GCGGAGGAGG CTGTGGCCAA GCTACAGGCT 
CGCATCGAAG ACCTGGAGCG GGCTATCAGC TCGGTGGAGC CCAAGGTCAT CGTGAAGGAG 
GTGAAGAAGG TGGAGCAGGA CCCAGGGCTC CTCCAGGAGT CCTCCAGGCT GAGGAGCCTC 
CTCGAGGAGG AGAGGACCAA GAACGCGACG CTGGCCAGGG AGCTGAGCGA CCTGCACAGC 
AAGTACAGCG TGGTGGAGAA GCAGAGGCCC AAAGTGCAGC TCCAGGAGCG CGTCCACGAG 
ATCTTCCAGG TGGATCCGGA GACAGAGCAG GAGATCACTC GGCTCAAGGC CAAGCTGCAG 
GAGATGGCGG GCAAGAGGAG CGGTGTGGAG AAGGAGGTGG AGAAGCTGCT GCCCGACCTG 
GAGGTCCTGC GGGCCCAGAA GCCCACGGTG GAGTACAAGG AGGTGACCCA GGAGGTGGTG 
AGGCATGAGA GGAGCCCCGA GGTGCTGCGT GAGATCGACC GCCTGAAGGC TCAGCTCAAC 
GAGCTCGTCA ACAGCCACGG GCGCTCCCAG GAGCAGCTCA TCCGCCTGCA GGGTGAGCGC 
GACGAGTGGA GGCGCGAGCG GGCCAAGGTG GAGACCAAGA CGGTGAGCAA GGAGGTGGTG 
CGCCACGAGA AGGACCCGGT GCTGGAGAAA GAAGCAGAGC GGCTCCGCCA GGAGGTGCGG 
GAGGCGGCCC AGAAGAGGCG GGCCGCGGAG GACGCGGTGT ACGAGCTGCA GAGCAAGCGC 
CTGCTGCTGG AGAGGAGGAA GCCCGAGGAG AAGGTGGTGG TGCAGGAGGT GGTGGTCACC 
CAGAAGGACC CGAAGCTGCG CGAGGAGCAC AGCCGGCTGA GCGGGAGCCT GGATGAGGAG 
GTGGGCCGGC GGCGCCAGCT AGAGCTTGAG GTGCAGCAGC TGCGGGCCGG CGTGGAGGAG 
CAGGAGGGCC TGCTCAGCTT CCAGGAGGAC CGCAGCAAGA AGCTGGCCGT GGAGAGGGAG 
CTGCGGCAGC TGACCTTGAG GATCCAGGAG CTCGAGAAGC GGCCTCCCAC GGTGCAGGAG 
AAGATCATCA TGGAGGAAGT GGTCAAGCTG GAGAAGGACC CGGACCTGGA GAAGTCCACG 
GAAGCCCTGC GGTGGGACCT GGACCAGGAG AAGACCCAGG TAACCGAGCT GAATCGGGAG 
TGCAAGAACC TGCAGGTCCA GATTGACGTC CTCCAGAAAG CCAAATCGCA GGAGAAGACC 
ATCTACAAGG AAGTGATCCG GGTGCAGAAG GACCGCGTCC TGGAAGATGA GCGGGCCCGC 
GTGTGGGAGA TGCTCAACAG GGAGCGCACG GCCCGGCAGG CCCGGGAGGA GGAGGCACGG 
CGCCTGCGGG AGCGCATTGA CCGGGCCGAG ACGCTGGGGA GAACCTGGTC CCGGGAGGAG 
TCCGAGCTGC AGAGGGCCCG GGACCAGGCC GACCAGGAGT GTGGGCGGCT GCAGCAGGAG 
CTGCGGGCTC TGGAGAGGCA GAAGCAGCAG CAGACACTGC AGCTGCAGGA GGAGTCGAAG 
CTGCTCAGCC AGAAGACGGA GAGCGAGCGA CAGAAGGCGG CCCAGCGGGG CCAGGAGCTC 
TCGCGGCTGG AGGCGGCCAT CCTCCGCGAG AAGGACCAGA TCTACGAGAA GGAGCGGACG 
CTCCGGGACC TCCACGCCAA GGTGAGCCGG GAGGAGCTCA GCCAGGAGAC CCAGACGCGA 
GAGACCAACC TTTCCACCAA GATCTCCATC CTGGAACCCG AGACGGGGAA GGACATGTCC 
CCATACGAGG CCTACAAGAG GGGCATCATC GACAGGGGCC AGTACTTGCA GCTGCAGGAG 
CTCGAGTGTG ACTGGGAGGA GGTCACCACC TCGGGGCCCT GTGGGGAGGA GTCTGTGCTC 
CTGGACCGCA AGAGCGGGAA GCAGTACTCC ATCGAGGCCG CCCTCCGCTG CCGGCGCATC 
TCTAAGGAGG AGTACCATCT GTACAAGGAC GGCCACCTGC CCATCTCCGA GTTTGCGCTG 
CTTGTAGCTG GGGAGACCAA GCCAAGCTCC TCACTCTCCA TCGGCTCTAT CATCTCCAAG 
TCCCCGCTCG CCTCCCCGGC CCCCCAGAGC ACCAGTTTCT TCTCTCCCAG CTTCTCTCTC 
GGGCTCGGTG ATGACAGCTT CCCTATCGCC GGGATCTATG ACACAACCAC AGACAACAAG 
TGCAGCATCA AGACGGCCGT GGCCAAGAAC ATGCTGGACC CCATCACTGG GCAGAAGCTA 
CTGGAGGCCC AGGCGGCCAC AGGGGGCATC GTGGACCTGC TCAGCCGTGA GCGCTACTCT 
GTGCACAAGG CGATGGAGAG GGGCCTGATC GAGAACACCT CCACACAGAG GCTGCTTAAC 
GCCCAGAAGG CCTTCACCGG CATCGAGGAC CCCGTCACCA AGAAGAGGCT CTCGGTGGGC 
GAGGCCGTCC AGAAGGGCTG GATGCCCCGG GAGAGCGTGC TCCCACACCT GCAGGTGCAG 
CACCTGACCG GGGGGCTCAT CGACCCCAAG AGGACAGGCC GCATCCCCAT CCAGCAGGCC 
CTCCTCTCCG GGATGATCAG TGAAGAGCTG GCCCAGCTCC TGCAGGACGA GTCCAGCTAC 
GAGAAGGATT TGACAGACCC CATCTCCAAG GAACGGCTGA GCTACAAGGA GGCCATGGGC 
CGCTGCCGCA AAGACCCCCT GAGCGGCCTG CTGCTCCTGC CAGCGGCACT GGAGGGGTAC 
CGCTGCTACC GCTCCGCCTC CCCCACCGTC CCGCGCTCCC TTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307676.1
CDS	109..6276
Translation	MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQGTQGGPGRGGCGARRSRFRLPTAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Target ORF information:

RefSeq Version	NM_001320747.2
Organism	Homo sapiens(human)
Definition	Homo sapiens envoplakin (EVPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320747.2

ORF Insert Sequence:

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGGTA CCCAGGGTGG CCCGGGGAGA 
GGTGGCTGTG GGGCTAGAAG GAGTAGATTC CGACTTCCGA CTGCTCCATC TGGCTCAGAC 
CTGGCCAACC CACAGGCCCA GAAGCTCCTG ACACAGATGA CCCGGCTGGA TGGAGACCTG 
GGACAGATAG AGAGGCAGGT GCTGGCCTGG GCGCGGGCCC CGCTGAGCCG CCCCACACCC 
TTGGAGGACT TGGAGGGCCG CATCCACAGC CATGAGGGCA CAGCCCAGCG CCTGCAGAGC 
CTGGGAACGG AGAAGGAGAC AGCCCAGAAG GAGTGCGAGG CGTTTCTGTC CACGCGGCCC 
GTGGGCCCCG CTGCCCTGCA GCTGCCCGTA GCCCTCAACA GCGTGAAGAA CAAGTTCAGT 
GACGTGCAGG TTCTGTGCAG CCTCTACGGG GAGAAAGCCA AGGCTGCCCT GGATCTGGAG 
CGGCAGATCC AGGATGCGGA CAGGGTCATC CGAGGCTTCG AGGCCACCCT GGTGCAGGAG 
GCCCCCATCC CTGCTGAACC GGGGGCTCTG CAGGAGAGGG TCAGCGAGCT GCAGCGCCAG 
CGGAGGGAGC TGCTGGAACA GCAGACCTGC GTGCTGCGGC TACACCGCGC GCTGAAGGCC 
TCGGAGCACG CATGCGCTGC CCTGCAGAAC AACTTCCAGG AGTTCTGCCA AGACCTGCCT 
CGCCAGCAGC GCCAGGTGCG AGCCCTCACC GACCGCTACC ACGCCGTAGG GGACCAGCTG 
GACCTGCGGG AGAAGGTGGT GCAGGATGCC GCCCTCACCT ACCAGCAGTT CAAGAACTGC 
AAGGATAACC TGAGCTCCTG GCTGGAGCAC CTGCCCCGCA GCCAGGTGCG GCCCAGCGAC 
GGCCCCAGCC AGATCGCCTA CAAGCTGCAG GCGCAGAAGA GGCTGACGCA GGAGATCCAG 
AGCCGAGAGC GGGACAGGGC CACAGCATCC CACCTCTCCC AGGCCCTGCA GGCAGCGCTC 
CAGGACTATG AGCTCCAGGC AGACACCTAC CGCTGCTCTT TGGAGCCCAC CCTGGCAGTG 
TCAGCCCCCA AGAGACCCCG AGTGGCTCCC CTGCAAGAGA GCATCCAAGC CCAGGAGAAG 
AACCTTGCAA AGGCCTATAC TGAGGTTGCA GCAGCACAGC AGCAGCTGCT CCAGCAGCTG 
GAGTTTGCTA GAAAAATGCT GGAGAAGAAG GAGCTCAGTG AGGACATCCG AAGGACCCAT 
GATGCAAAGC AGGGCTCCGA GAGCCCTGCC CAAGCAGGGA GAGAGTCAGA GGCCCTGAAG 
GCCCAGCTGG AAGAGGAGAG GAAGCGGGTG GCCCGGGTGC AGCATGAGCT GGAGGCGCAG 
AGGAGCCAAC TGCTGCAGCT GAGGACCCAG CGGCCCTTGG AGAGGCTGGA GGAGAAGGAA 
GTGGTAGAGT TCTACCGGGA CCCCCAGCTG GAGGGCAGCC TGTCCAGGGT GAAGGCCCAG 
GTGGAGGAGG AGGGCAAGCG GCGGGCTGGC CTGCAGGCAG ACCTGGAAGT GGCAGCCCAG 
AAGGTCGTGC AGCTGGAAAG CAAGAGGAAG ACCATGCAGC CTCATCTGCT GACCAAGGAG 
GTCACCCAGG TGGAGAGGGA CCCCGGCCTG GACAGCCAGG CGGCCCAGCT CAGGATCCAG 
ATCCAGCAGC TCCGCGGGGA GGATGCCGTC ATCTCGGCCC GGCTGGAAGG GCTGAAGAAG 
GAGCTACTGG CCCTTGAGAA GAGGGAGGTG GACGTGAAGG AGAAGGTCGT GGTGAAAGAG 
GTAGTCAAGG TGGAGAAGAA TCTGGAAATG GTCAAGGCAG CCCAGGCTCT GAGGCTGCAG 
ATGGAGGAGG ATGCTGCGCG GAGGAAGCAG GCGGAGGAGG CTGTGGCCAA GCTACAGGCT 
CGCATCGAAG ACCTGGAGCG GGCTATCAGC TCGGTGGAGC CCAAGGTCAT CGTGAAGGAG 
GTGAAGAAGG TGGAGCAGGA CCCAGGGCTC CTCCAGGAGT CCTCCAGGCT GAGGAGCCTC 
CTCGAGGAGG AGAGGACCAA GAACGCGACG CTGGCCAGGG AGCTGAGCGA CCTGCACAGC 
AAGTACAGCG TGGTGGAGAA GCAGAGGCCC AAAGTGCAGC TCCAGGAGCG CGTCCACGAG 
ATCTTCCAGG TGGATCCGGA GACAGAGCAG GAGATCACTC GGCTCAAGGC CAAGCTGCAG 
GAGATGGCGG GCAAGAGGAG CGGTGTGGAG AAGGAGGTGG AGAAGCTGCT GCCCGACCTG 
GAGGTCCTGC GGGCCCAGAA GCCCACGGTG GAGTACAAGG AGGTGACCCA GGAGGTGGTG 
AGGCATGAGA GGAGCCCCGA GGTGCTGCGT GAGATCGACC GCCTGAAGGC TCAGCTCAAC 
GAGCTCGTCA ACAGCCACGG GCGCTCCCAG GAGCAGCTCA TCCGCCTGCA GGGTGAGCGC 
GACGAGTGGA GGCGCGAGCG GGCCAAGGTG GAGACCAAGA CGGTGAGCAA GGAGGTGGTG 
CGCCACGAGA AGGACCCGGT GCTGGAGAAA GAAGCAGAGC GGCTCCGCCA GGAGGTGCGG 
GAGGCGGCCC AGAAGAGGCG GGCCGCGGAG GACGCGGTGT ACGAGCTGCA GAGCAAGCGC 
CTGCTGCTGG AGAGGAGGAA GCCCGAGGAG AAGGTGGTGG TGCAGGAGGT GGTGGTCACC 
CAGAAGGACC CGAAGCTGCG CGAGGAGCAC AGCCGGCTGA GCGGGAGCCT GGATGAGGAG 
GTGGGCCGGC GGCGCCAGCT AGAGCTTGAG GTGCAGCAGC TGCGGGCCGG CGTGGAGGAG 
CAGGAGGGCC TGCTCAGCTT CCAGGAGGAC CGCAGCAAGA AGCTGGCCGT GGAGAGGGAG 
CTGCGGCAGC TGACCTTGAG GATCCAGGAG CTCGAGAAGC GGCCTCCCAC GGTGCAGGAG 
AAGATCATCA TGGAGGAAGT GGTCAAGCTG GAGAAGGACC CGGACCTGGA GAAGTCCACG 
GAAGCCCTGC GGTGGGACCT GGACCAGGAG AAGACCCAGG TAACCGAGCT GAATCGGGAG 
TGCAAGAACC TGCAGGTCCA GATTGACGTC CTCCAGAAAG CCAAATCGCA GGAGAAGACC 
ATCTACAAGG AAGTGATCCG GGTGCAGAAG GACCGCGTCC TGGAAGATGA GCGGGCCCGC 
GTGTGGGAGA TGCTCAACAG GGAGCGCACG GCCCGGCAGG CCCGGGAGGA GGAGGCACGG 
CGCCTGCGGG AGCGCATTGA CCGGGCCGAG ACGCTGGGGA GAACCTGGTC CCGGGAGGAG 
TCCGAGCTGC AGAGGGCCCG GGACCAGGCC GACCAGGAGT GTGGGCGGCT GCAGCAGGAG 
CTGCGGGCTC TGGAGAGGCA GAAGCAGCAG CAGACACTGC AGCTGCAGGA GGAGTCGAAG 
CTGCTCAGCC AGAAGACGGA GAGCGAGCGA CAGAAGGCGG CCCAGCGGGG CCAGGAGCTC 
TCGCGGCTGG AGGCGGCCAT CCTCCGCGAG AAGGACCAGA TCTACGAGAA GGAGCGGACG 
CTCCGGGACC TCCACGCCAA GGTGAGCCGG GAGGAGCTCA GCCAGGAGAC CCAGACGCGA 
GAGACCAACC TTTCCACCAA GATCTCCATC CTGGAACCCG AGACGGGGAA GGACATGTCC 
CCATACGAGG CCTACAAGAG GGGCATCATC GACAGGGGCC AGTACTTGCA GCTGCAGGAG 
CTCGAGTGTG ACTGGGAGGA GGTCACCACC TCGGGGCCCT GTGGGGAGGA GTCTGTGCTC 
CTGGACCGCA AGAGCGGGAA GCAGTACTCC ATCGAGGCCG CCCTCCGCTG CCGGCGCATC 
TCTAAGGAGG AGTACCATCT GTACAAGGAC GGCCACCTGC CCATCTCCGA GTTTGCGCTG 
CTTGTAGCTG GGGAGACCAA GCCAAGCTCC TCACTCTCCA TCGGCTCTAT CATCTCCAAG 
TCCCCGCTCG CCTCCCCGGC CCCCCAGAGC ACCAGTTTCT TCTCTCCCAG CTTCTCTCTC 
GGGCTCGGTG ATGACAGCTT CCCTATCGCC GGGATCTATG ACACAACCAC AGACAACAAG 
TGCAGCATCA AGACGGCCGT GGCCAAGAAC ATGCTGGACC CCATCACTGG GCAGAAGCTA 
CTGGAGGCCC AGGCGGCCAC AGGGGGCATC GTGGACCTGC TCAGCCGTGA GCGCTACTCT 
GTGCACAAGG CGATGGAGAG GGGCCTGATC GAGAACACCT CCACACAGAG GCTGCTTAAC 
GCCCAGAAGG CCTTCACCGG CATCGAGGAC CCCGTCACCA AGAAGAGGCT CTCGGTGGGC 
GAGGCCGTCC AGAAGGGCTG GATGCCCCGG GAGAGCGTGC TCCCACACCT GCAGGTGCAG 
CACCTGACCG GGGGGCTCAT CGACCCCAAG AGGACAGGCC GCATCCCCAT CCAGCAGGCC 
CTCCTCTCCG GGATGATCAG TGAAGAGCTG GCCCAGCTCC TGCAGGACGA GTCCAGCTAC 
GAGAAGGATT TGACAGACCC CATCTCCAAG GAACGGCTGA GCTACAAGGA GGCCATGGGC 
CGCTGCCGCA AAGACCCCCT GAGCGGCCTG CTGCTCCTGC CAGCGGCACT GGAGGGGTAC 
CGCTGCTACC GCTCCGCCTC CCCCACCGTC CCGCGCTCCC TTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVPL ( NM_001988.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001979.2 Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.

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CloneID	OHu08934
Clone ID Related Accession (Same CDS sequence)	NM_001988.4 , NM_001988.2 , NM_001988.3
Accession Version	NM_001988.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	envoplakin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA449929.1, U53786.1, BC126103.1 and BX114092.1. On Jun 2, 2019 this sequence version replaced NM_001988.3. Transcript Variant: This variant (2) uses an alternate in-frame slice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53786.1, BC126105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001979.2
CDS	109..6210
Translation	MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Target ORF information:

RefSeq Version	NM_001988.4
Organism	Homo sapiens(human)
Definition	Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001988.4

ORF Insert Sequence:

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVPL ( NM_001988.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001979.2 Homo sapiens envoplakin (EVPL), mRNA.

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CloneID	OHu08934
Clone ID Related Accession (Same CDS sequence)	NM_001988.4 , NM_001988.2 , NM_001988.3
Accession Version	NM_001988.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-24
Organism	Homo sapiens(Human)
Product	envoplakin
Comment

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001979.2

Translation

MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Target ORF information:

RefSeq Version	NM_001988.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens envoplakin (EVPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001988.2

ORF Insert Sequence:

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVPL ( NM_001988.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001979.2 Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.

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CloneID	OHu08934
Clone ID Related Accession (Same CDS sequence)	NM_001988.4 , NM_001988.2 , NM_001988.3
Accession Version	NM_001988.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	envoplakin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA449929.1, U53786.1, BC126103.1 and BX114092.1. On Mar 4, 2016 this sequence version replaced NM_001988.2. Transcript Variant: This variant (2) uses an alternate in-frame slice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53786.1, BC126103.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001979.2
CDS	255..6356
Translation	MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Target ORF information:

RefSeq Version	NM_001988.3
Organism	Homo sapiens(human)
Definition	Homo sapiens envoplakin (EVPL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001988.3

ORF Insert Sequence:

ATGTTCAAGG GGCTGAGCAA AGGCTCCCAG GGGAAGGGGT CCCCCAAGGG CTCCCCCGCC 
AAGGGGTCCC CCAAAGGCTC CCCCAGCAGG CACAGCCGGG CTGCCACCCA GGAGCTGGCC 
CTTCTCATCT CCCGCATGCA AGCCAACGCC GACCAGGTGG AGCGGGACAT CCTGGAGACG 
CAGAAGAGGC TGCAGCAGGA CCGGCTGAAC AGTGAGCAGA GCCAGGCCCT GCAGCACCAG 
CAGGAGACGG GCCGCAGCCT GAAGGAGGCT GAGGTGCTGC TCAAGGACCT CTTCCTGGAC 
GTGGACAAGG CCCGGCGGCT CAAGCACCCG CAGGCTGAGG AGATTGAGAA GGACATCAAG 
CAGCTGCACG AGCGGGTGAC CCAGGAGTGT GCGGAGTACC GTGCCCTGTA CGAGAAGATG 
GTGCTGCCCC CCGACGTGGG ACCCAGGGTC GACTGGGCAC GCGTGCTGGA GCAGAAACAG 
AAGCAGGTCT GCGCAGGCCA GTACGGGCCG GGCATGGCGG AGCTGGAGCA ACAGATCGCC 
GAGCACAACA TCCTGCAGAA GGAGATCGAC GCCTATGGGC AGCAGCTGCG GAGCCTCGTG 
GGGCCGGATG CAGCCACCAT CCGGAGCCAA TACCGAGACC TACTGAAGGC GGCGTCGTGG 
CGCGGGCAGA GCCTGGGCAG CCTGTACACG CACCTCCAGG GCTGCACGCG GCAGCTGAGC 
GCCCTGGCTG AGCAGCAGCG CCGCATCCTG CAGCAGGACT GGAGCGACCT CATGGCCGAC 
CCTGCGGGCG TGCGGCGGGA GTACGAGCAC TTCAAGCAGC ACGAGCTGCT GAGCCAGGAG 
CAGAGCGTGA ACCAGCTGGA GGACGACGGC GAGCGCATGG TGGAGCTGCG GCACCCCGCG 
GTGGGGCCCA TCCAGGCCCA CCAGGAGGCC CTGAAGATGG AGTGGCAGAA CTTCCTGAAC 
CTGTGTATCT GCCAGGAGAC CCAGCTGCAG CACGTGGAGG ACTACCGCCG GTTCCAGGAA 
GAGGCCGACT CAGTCAGCCA GACCCTGGCG AAGCTCAACT CCAACTTGGA TGCCAAGTAC 
AGCCCTGCAC CTGGGGGCCC CCCTGGCGCC CCCACAGAGC TGCTGCAACA GCTGGAGGCA 
GAGGAAAAAC GGCTGGCCGT CACCGAGAGG GCCACTGGGG ACCTGCAGCG GCGAAGCCGG 
GATGTGGCCC CTCTGCCACA GCGAAGAAAC CCCCCTCAGC AGCCCCTGCA CGTGGACAGC 
ATCTGCGACT GGGACTCAGG AGAAGTGCAG CTGCTGCAGG GTGAGCGGTA TAAGCTGGTA 
GATAACACTG ACCCGCACGC CTGGGTCGTG CAGGGCCCTG GCGGGGAGAC CAAGCGTGCT 
CCCGCCGCCT GCTTCTGCAT CCCAGCACCA GACCCTGATG CTGTGGCCAG GGCCTCCCGG 
CTGGCCTCAG AGCTGCAGGC CCTGAAGCAG AAATTGGCCA CAGTCCAGAG CCGCCTGAAG 
GCCAGTGCTG TGGAGTCTCT TCGGCCCAGC CAGCAGGCTC CATCTGGCTC AGACCTGGCC 
AACCCACAGG CCCAGAAGCT CCTGACACAG ATGACCCGGC TGGATGGAGA CCTGGGACAG 
ATAGAGAGGC AGGTGCTGGC CTGGGCGCGG GCCCCGCTGA GCCGCCCCAC ACCCTTGGAG 
GACTTGGAGG GCCGCATCCA CAGCCATGAG GGCACAGCCC AGCGCCTGCA GAGCCTGGGA 
ACGGAGAAGG AGACAGCCCA GAAGGAGTGC GAGGCGTTTC TGTCCACGCG GCCCGTGGGC 
CCCGCTGCCC TGCAGCTGCC CGTAGCCCTC AACAGCGTGA AGAACAAGTT CAGTGACGTG 
CAGGTTCTGT GCAGCCTCTA CGGGGAGAAA GCCAAGGCTG CCCTGGATCT GGAGCGGCAG 
ATCCAGGATG CGGACAGGGT CATCCGAGGC TTCGAGGCCA CCCTGGTGCA GGAGGCCCCC 
ATCCCTGCTG AACCGGGGGC TCTGCAGGAG AGGGTCAGCG AGCTGCAGCG CCAGCGGAGG 
GAGCTGCTGG AACAGCAGAC CTGCGTGCTG CGGCTACACC GCGCGCTGAA GGCCTCGGAG 
CACGCATGCG CTGCCCTGCA GAACAACTTC CAGGAGTTCT GCCAAGACCT GCCTCGCCAG 
CAGCGCCAGG TGCGAGCCCT CACCGACCGC TACCACGCCG TAGGGGACCA GCTGGACCTG 
CGGGAGAAGG TGGTGCAGGA TGCCGCCCTC ACCTACCAGC AGTTCAAGAA CTGCAAGGAT 
AACCTGAGCT CCTGGCTGGA GCACCTGCCC CGCAGCCAGG TGCGGCCCAG CGACGGCCCC 
AGCCAGATCG CCTACAAGCT GCAGGCGCAG AAGAGGCTGA CGCAGGAGAT CCAGAGCCGA 
GAGCGGGACA GGGCCACAGC ATCCCACCTC TCCCAGGCCC TGCAGGCAGC GCTCCAGGAC 
TATGAGCTCC AGGCAGACAC CTACCGCTGC TCTTTGGAGC CCACCCTGGC AGTGTCAGCC 
CCCAAGAGAC CCCGAGTGGC TCCCCTGCAA GAGAGCATCC AAGCCCAGGA GAAGAACCTT 
GCAAAGGCCT ATACTGAGGT TGCAGCAGCA CAGCAGCAGC TGCTCCAGCA GCTGGAGTTT 
GCTAGAAAAA TGCTGGAGAA GAAGGAGCTC AGTGAGGACA TCCGAAGGAC CCATGATGCA 
AAGCAGGGCT CCGAGAGCCC TGCCCAAGCA GGGAGAGAGT CAGAGGCCCT GAAGGCCCAG 
CTGGAAGAGG AGAGGAAGCG GGTGGCCCGG GTGCAGCATG AGCTGGAGGC GCAGAGGAGC 
CAACTGCTGC AGCTGAGGAC CCAGCGGCCC TTGGAGAGGC TGGAGGAGAA GGAAGTGGTA 
GAGTTCTACC GGGACCCCCA GCTGGAGGGC AGCCTGTCCA GGGTGAAGGC CCAGGTGGAG 
GAGGAGGGCA AGCGGCGGGC TGGCCTGCAG GCAGACCTGG AAGTGGCAGC CCAGAAGGTC 
GTGCAGCTGG AAAGCAAGAG GAAGACCATG CAGCCTCATC TGCTGACCAA GGAGGTCACC 
CAGGTGGAGA GGGACCCCGG CCTGGACAGC CAGGCGGCCC AGCTCAGGAT CCAGATCCAG 
CAGCTCCGCG GGGAGGATGC CGTCATCTCG GCCCGGCTGG AAGGGCTGAA GAAGGAGCTA 
CTGGCCCTTG AGAAGAGGGA GGTGGACGTG AAGGAGAAGG TCGTGGTGAA AGAGGTAGTC 
AAGGTGGAGA AGAATCTGGA AATGGTCAAG GCAGCCCAGG CTCTGAGGCT GCAGATGGAG 
GAGGATGCTG CGCGGAGGAA GCAGGCGGAG GAGGCTGTGG CCAAGCTACA GGCTCGCATC 
GAAGACCTGG AGCGGGCTAT CAGCTCGGTG GAGCCCAAGG TCATCGTGAA GGAGGTGAAG 
AAGGTGGAGC AGGACCCAGG GCTCCTCCAG GAGTCCTCCA GGCTGAGGAG CCTCCTCGAG 
GAGGAGAGGA CCAAGAACGC GACGCTGGCC AGGGAGCTGA GCGACCTGCA CAGCAAGTAC 
AGCGTGGTGG AGAAGCAGAG GCCCAAAGTG CAGCTCCAGG AGCGCGTCCA CGAGATCTTC 
CAGGTGGATC CGGAGACAGA GCAGGAGATC ACTCGGCTCA AGGCCAAGCT GCAGGAGATG 
GCGGGCAAGA GGAGCGGTGT GGAGAAGGAG GTGGAGAAGC TGCTGCCCGA CCTGGAGGTC 
CTGCGGGCCC AGAAGCCCAC GGTGGAGTAC AAGGAGGTGA CCCAGGAGGT GGTGAGGCAT 
GAGAGGAGCC CCGAGGTGCT GCGTGAGATC GACCGCCTGA AGGCTCAGCT CAACGAGCTC 
GTCAACAGCC ACGGGCGCTC CCAGGAGCAG CTCATCCGCC TGCAGGGTGA GCGCGACGAG 
TGGAGGCGCG AGCGGGCCAA GGTGGAGACC AAGACGGTGA GCAAGGAGGT GGTGCGCCAC 
GAGAAGGACC CGGTGCTGGA GAAAGAAGCA GAGCGGCTCC GCCAGGAGGT GCGGGAGGCG 
GCCCAGAAGA GGCGGGCCGC GGAGGACGCG GTGTACGAGC TGCAGAGCAA GCGCCTGCTG 
CTGGAGAGGA GGAAGCCCGA GGAGAAGGTG GTGGTGCAGG AGGTGGTGGT CACCCAGAAG 
GACCCGAAGC TGCGCGAGGA GCACAGCCGG CTGAGCGGGA GCCTGGATGA GGAGGTGGGC 
CGGCGGCGCC AGCTAGAGCT TGAGGTGCAG CAGCTGCGGG CCGGCGTGGA GGAGCAGGAG 
GGCCTGCTCA GCTTCCAGGA GGACCGCAGC AAGAAGCTGG CCGTGGAGAG GGAGCTGCGG 
CAGCTGACCT TGAGGATCCA GGAGCTCGAG AAGCGGCCTC CCACGGTGCA GGAGAAGATC 
ATCATGGAGG AAGTGGTCAA GCTGGAGAAG GACCCGGACC TGGAGAAGTC CACGGAAGCC 
CTGCGGTGGG ACCTGGACCA GGAGAAGACC CAGGTAACCG AGCTGAATCG GGAGTGCAAG 
AACCTGCAGG TCCAGATTGA CGTCCTCCAG AAAGCCAAAT CGCAGGAGAA GACCATCTAC 
AAGGAAGTGA TCCGGGTGCA GAAGGACCGC GTCCTGGAAG ATGAGCGGGC CCGCGTGTGG 
GAGATGCTCA ACAGGGAGCG CACGGCCCGG CAGGCCCGGG AGGAGGAGGC ACGGCGCCTG 
CGGGAGCGCA TTGACCGGGC CGAGACGCTG GGGAGAACCT GGTCCCGGGA GGAGTCCGAG 
CTGCAGAGGG CCCGGGACCA GGCCGACCAG GAGTGTGGGC GGCTGCAGCA GGAGCTGCGG 
GCTCTGGAGA GGCAGAAGCA GCAGCAGACA CTGCAGCTGC AGGAGGAGTC GAAGCTGCTC 
AGCCAGAAGA CGGAGAGCGA GCGACAGAAG GCGGCCCAGC GGGGCCAGGA GCTCTCGCGG 
CTGGAGGCGG CCATCCTCCG CGAGAAGGAC CAGATCTACG AGAAGGAGCG GACGCTCCGG 
GACCTCCACG CCAAGGTGAG CCGGGAGGAG CTCAGCCAGG AGACCCAGAC GCGAGAGACC 
AACCTTTCCA CCAAGATCTC CATCCTGGAA CCCGAGACGG GGAAGGACAT GTCCCCATAC 
GAGGCCTACA AGAGGGGCAT CATCGACAGG GGCCAGTACT TGCAGCTGCA GGAGCTCGAG 
TGTGACTGGG AGGAGGTCAC CACCTCGGGG CCCTGTGGGG AGGAGTCTGT GCTCCTGGAC 
CGCAAGAGCG GGAAGCAGTA CTCCATCGAG GCCGCCCTCC GCTGCCGGCG CATCTCTAAG 
GAGGAGTACC ATCTGTACAA GGACGGCCAC CTGCCCATCT CCGAGTTTGC GCTGCTTGTA 
GCTGGGGAGA CCAAGCCAAG CTCCTCACTC TCCATCGGCT CTATCATCTC CAAGTCCCCG 
CTCGCCTCCC CGGCCCCCCA GAGCACCAGT TTCTTCTCTC CCAGCTTCTC TCTCGGGCTC 
GGTGATGACA GCTTCCCTAT CGCCGGGATC TATGACACAA CCACAGACAA CAAGTGCAGC 
ATCAAGACGG CCGTGGCCAA GAACATGCTG GACCCCATCA CTGGGCAGAA GCTACTGGAG 
GCCCAGGCGG CCACAGGGGG CATCGTGGAC CTGCTCAGCC GTGAGCGCTA CTCTGTGCAC 
AAGGCGATGG AGAGGGGCCT GATCGAGAAC ACCTCCACAC AGAGGCTGCT TAACGCCCAG 
AAGGCCTTCA CCGGCATCGA GGACCCCGTC ACCAAGAAGA GGCTCTCGGT GGGCGAGGCC 
GTCCAGAAGG GCTGGATGCC CCGGGAGAGC GTGCTCCCAC ACCTGCAGGT GCAGCACCTG 
ACCGGGGGGC TCATCGACCC CAAGAGGACA GGCCGCATCC CCATCCAGCA GGCCCTCCTC 
TCCGGGATGA TCAGTGAAGA GCTGGCCCAG CTCCTGCAGG ACGAGTCCAG CTACGAGAAG 
GATTTGACAG ACCCCATCTC CAAGGAACGG CTGAGCTACA AGGAGGCCAT GGGCCGCTGC 
CGCAAAGACC CCCTGAGCGG CCTGCTGCTC CTGCCAGCGG CACTGGAGGG GTACCGCTGC 
TACCGCTCCG CCTCCCCCAC CGTCCCGCGC TCCCTTCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extremities of the N-terminus of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus.
Journal of dermatological science84(1)24-29(2016 Oct)
Wang X,Chen T,Zhao J,Peng Y,Chen X,Tu P,Zhu X,Liu Z,Wang M

Selected single-nucleotide polymorphisms in FOXE1, SERPINA5, FTO, EVPL, TICAM1 and SCARB1 are associated with papillary and follicular thyroid cancer risk: replication study in a German population.
Carcinogenesis37(7)677-684(2016 Jul)
Sigurdson AJ,Brenner AV,Roach JA,Goudeva L,M?ller JA,Nerlich K,Reiners C,Schwab R,Pfeiffer L,Waldenberger M,Braganza M,Xu L,Sturgis EM,Yeager M,Chanock SJ,Pfeiffer RM,Abend M,Port M

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Journal of proteome research11(4)2533-43(2012 Apr)
Martins-de-Souza D,Guest PC,Mann DM,Roeber S,Rahmoune H,Bauder C,Kretzschmar H,Volk B,Baborie A,Bahn S

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

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EVPL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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