EPHB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPHB3
Entrez Gene ID	2049
Full Name	EPH receptor B3
Synonyms	EK2,ETK2,HEK2,TYRO6
General protein information	Preferred Names EPH receptor B3 Names ephrin type-B receptor 3 EPH-like tyrosine kinase 2 embryonic kinase 2 human embryo kinase 2 tyrosine-protein kinase TYRO6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q27.1
Summary	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010].
Disorder MIM:	601839

mRNA and Protein(s)

mRNA	Protein	Name
NM_004443.3	NP_004434.2	ephrin type-B receptor 3 precursor
NM_004443.4	NP_004434.2	ephrin type-B receptor 3

Pathways from BioSystems

Homologies

	EPHB3	XP_001097522.2
Rattus norvegicus (Norway rat)	Ephb3	NP_001099338.1
Gallus gallus (chicken)	EPHB3	XP_422762.4
Homo sapiens (human)	EPHB3	NP_004434.2
Pan troglodytes (chimpanzee)	EPHB3	XP_003310198.1
Bos taurus (cattle)	EPHB3	NP_001179725.1
Danio rerio (zebrafish)	LOC555957	XP_683712.6
Canis lupus familiaris (dog)	EPHB3	XP_545232.3
Mus musculus (house mouse)	Ephb3	NP_034273.1
Xenopus tropicalis (tropical clawed frog)	LOC100489199	XP_002935194.1

Gene Ontology

The following EPHB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55774	NM_004443.4 Latest version!	Homo sapiens EPH receptor B3 (EPHB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu55774	NM_004443.3	Homo sapiens EPH receptor B3 (EPHB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPHB3 ( NM_004443.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004434.2 Homo sapiens EPH receptor B3 (EPHB3), mRNA.

Price & Availability↑

CloneID	OHu55774
Clone ID Related Accession (Same CDS sequence)	NM_004443.3 , NM_004443.4
Accession Version	NM_004443.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Homo sapiens(human)
Product	ephrin type-B receptor 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB993133.1, BC052968.1 and X75208.1. On Aug 12, 2003 this sequence version replaced NM_004443.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052968.1, X75208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGAG CCCGCCCGCC GCCGCCGCCG TCGCCGCCGC CGGGGCTTCT GCCGCTGCTC 
CCTCCGCTGC TGCTGCTGCC GCTGCTGCTG CTGCCCGCCG GCTGCCGGGC GCTGGAAGAG 
ACCCTCATGG ACACAAAATG GGTAACATCT GAGTTGGCGT GGACATCTCA TCCAGAAAGT 
GGGTGGGAAG AGGTGAGTGG CTACGATGAG GCCATGAATC CCATCCGCAC ATACCAGGTG 
TGTAATGTGC GCGAGTCAAG CCAGAACAAC TGGCTTCGCA CGGGGTTCAT CTGGCGGCGG 
GATGTGCAGC GGGTCTACGT GGAGCTCAAG TTCACTGTGC GTGACTGCAA CAGCATCCCC 
AACATCCCCG GCTCCTGCAA GGAGACCTTC AACCTCTTCT ACTACGAGGC TGACAGCGAT 
GTGGCCTCAG CCTCCTCCCC CTTCTGGATG GAGAACCCCT ACGTGAAAGT GGACACCATT 
GCACCCGATG AGAGCTTCTC GCGGCTGGAT GCCGGCCGTG TCAACACCAA GGTGCGCAGC 
TTTGGGCCAC TTTCCAAGGC TGGCTTCTAC CTGGCCTTCC AGGACCAGGG CGCCTGCATG 
TCGCTCATCT CCGTGCGCGC CTTCTACAAG AAGTGTGCAT CCACCACCGC AGGCTTCGCA 
CTCTTCCCCG AGACCCTCAC TGGGGCGGAG CCCACCTCGC TGGTCATTGC TCCTGGCACC 
TGCATCCCTA ACGCCGTGGA GGTGTCGGTG CCACTCAAGC TCTACTGCAA CGGCGATGGG 
GAGTGGATGG TGCCTGTGGG TGCCTGCACC TGTGCCACCG GCCATGAGCC AGCTGCCAAG 
GAGTCCCAGT GCCGCCCCTG TCCCCCTGGG AGCTACAAGG CGAAGCAGGG AGAGGGGCCC 
TGCCTCCCAT GTCCCCCCAA CAGCCGTACC ACCTCCCCAG CCGCCAGCAT CTGCACCTGC 
CACAATAACT TCTACCGTGC AGACTCGGAC TCTGCGGACA GTGCCTGTAC CACCGTGCCA 
TCTCCACCCC GAGGTGTGAT CTCCAATGTG AATGAAACCT CACTGATCCT CGAGTGGAGT 
GAGCCCCGGG ACCTGGGTGG CCGGGATGAC CTCCTGTACA ATGTCATCTG CAAGAAGTGC 
CATGGGGCTG GAGGGGCCTC AGCCTGCTCA CGCTGTGATG ACAACGTGGA GTTTGTGCCT 
CGGCAGCTGG GCCTGACGGA GCGCCGGGTC CACATCAGCC ATCTGCTGGC CCACACGCGC 
TACACCTTTG AGGTGCAGGC GGTCAACGGT GTCTCGGGCA AGAGCCCTCT GCCGCCTCGT 
TATGCGGCCG TGAATATCAC CACAAACCAG GCTGCCCCGT CTGAAGTGCC CACACTACGC 
CTGCACAGCA GCTCAGGCAG CAGCCTCACC CTATCCTGGG CACCCCCAGA GCGGCCCAAC 
GGAGTCATCC TGGACTACGA GATGAAGTAC TTTGAGAAGA GCGAGGGCAT CGCCTCCACA 
GTGACCAGCC AGATGAACTC CGTGCAGCTG GACGGGCTTC GGCCTGACGC CCGCTATGTG 
GTCCAGGTCC GTGCCCGCAC AGTAGCTGGC TATGGGCAGT ACAGCCGCCC TGCCGAGTTT 
GAGACCACAA GTGAGAGAGG CTCTGGGGCC CAGCAGCTCC AGGAGCAGCT TCCCCTCATC 
GTGGGCTCCG CTACAGCTGG GCTTGTCTTC GTGGTGGCTG TCGTGGTCAT CGCTATCGTC 
TGCCTCAGGA AGCAGCGACA CGGCTCTGAT TCGGAGTACA CGGAGAAGCT GCAGCAGTAC 
ATTGCTCCTG GAATGAAGGT TTATATTGAC CCTTTTACCT ACGAGGACCC TAATGAGGCT 
GTTCGGGAGT TTGCCAAGGA GATCGACGTG TCCTGCGTCA AGATCGAGGA GGTGATCGGA 
GCTGGGGAAT TTGGGGAAGT GTGCCGTGGT CGACTGAAAC AGCCTGGCCG CCGAGAGGTG 
TTTGTGGCCA TCAAGACGCT GAAGGTGGGC TACACCGAGA GGCAGCGGCG GGACTTCCTA 
AGCGAGGCCT CCATCATGGG TCAGTTTGAT CACCCCAATA TAATCCGGCT CGAGGGCGTG 
GTCACCAAAA GTCGGCCAGT TATGATCCTC ACTGAGTTCA TGGAAAACTG CGCCCTGGAC 
TCCTTCCTCC GGCTCAACGA TGGGCAGTTC ACGGTCATCC AGCTGGTGGG CATGTTGCGG 
GGCATTGCTG CCGGCATGAA GTACCTGTCC GAGATGAACT ATGTGCACCG CGACCTGGCT 
GCTCGCAACA TCCTTGTCAA CAGCAACCTG GTCTGCAAAG TCTCAGACTT TGGCCTCTCC 
CGCTTCCTGG AGGATGACCC CTCCGATCCT ACCTACACCA GTTCCCTGGG CGGGAAGATC 
CCCATCCGCT GGACTGCCCC AGAGGCCATA GCCTATCGGA AGTTCACTTC TGCTAGTGAT 
GTCTGGAGCT ACGGAATTGT CATGTGGGAG GTCATGAGCT ATGGAGAGCG ACCCTACTGG 
GACATGAGCA ACCAGGATGT CATCAATGCC GTGGAGCAGG ATTACCGGCT GCCACCACCC 
ATGGACTGTC CCACAGCACT GCACCAGCTC ATGCTGGACT GCTGGGTGCG GGACCGGAAC 
CTCAGGCCCA AATTCTCCCA GATTGTCAAT ACCCTGGACA AGCTCATCCG CAATGCTGCC 
AGCCTCAAGG TCATTGCCAG CGCTCAGTCT GGCATGTCAC AGCCCCTCCT GGACCGCACG 
GTCCCAGATT ACACAACCTT CACGACAGTT GGTGATTGGC TGGATGCCAT CAAGATGGGG 
CGGTACAAGG AGAGCTTCGT CAGTGCGGGG TTTGCATCTT TTGACCTGGT GGCCCAGATG 
ACGGCAGAAG ACCTGCTCCG TATTGGGGTC ACCCTGGCCG GCCACCAGAA GAAGATCCTG 
AGCAGTATCC AGGACATGCG GCTGCAGATG AACCAGACGC TGCCTGTGCA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004434.2
CDS	438..3434
Translation	MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSATAGLVFVVAVVVIAIVCLRKQRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV

Target ORF information:

RefSeq Version	NM_004443.3
Organism	Homo sapiens(human)
Definition	Homo sapiens EPH receptor B3 (EPHB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004443.3

ORF Insert Sequence:

ATGGCCAGAG CCCGCCCGCC GCCGCCGCCG TCGCCGCCGC CGGGGCTTCT GCCGCTGCTC 
CCTCCGCTGC TGCTGCTGCC GCTGCTGCTG CTGCCCGCCG GCTGCCGGGC GCTGGAAGAG 
ACCCTCATGG ACACAAAATG GGTAACATCT GAGTTGGCGT GGACATCTCA TCCAGAAAGT 
GGGTGGGAAG AGGTGAGTGG CTACGATGAG GCCATGAATC CCATCCGCAC ATACCAGGTG 
TGTAATGTGC GCGAGTCAAG CCAGAACAAC TGGCTTCGCA CGGGGTTCAT CTGGCGGCGG 
GATGTGCAGC GGGTCTACGT GGAGCTCAAG TTCACTGTGC GTGACTGCAA CAGCATCCCC 
AACATCCCCG GCTCCTGCAA GGAGACCTTC AACCTCTTCT ACTACGAGGC TGACAGCGAT 
GTGGCCTCAG CCTCCTCCCC CTTCTGGATG GAGAACCCCT ACGTGAAAGT GGACACCATT 
GCACCCGATG AGAGCTTCTC GCGGCTGGAT GCCGGCCGTG TCAACACCAA GGTGCGCAGC 
TTTGGGCCAC TTTCCAAGGC TGGCTTCTAC CTGGCCTTCC AGGACCAGGG CGCCTGCATG 
TCGCTCATCT CCGTGCGCGC CTTCTACAAG AAGTGTGCAT CCACCACCGC AGGCTTCGCA 
CTCTTCCCCG AGACCCTCAC TGGGGCGGAG CCCACCTCGC TGGTCATTGC TCCTGGCACC 
TGCATCCCTA ACGCCGTGGA GGTGTCGGTG CCACTCAAGC TCTACTGCAA CGGCGATGGG 
GAGTGGATGG TGCCTGTGGG TGCCTGCACC TGTGCCACCG GCCATGAGCC AGCTGCCAAG 
GAGTCCCAGT GCCGCCCCTG TCCCCCTGGG AGCTACAAGG CGAAGCAGGG AGAGGGGCCC 
TGCCTCCCAT GTCCCCCCAA CAGCCGTACC ACCTCCCCAG CCGCCAGCAT CTGCACCTGC 
CACAATAACT TCTACCGTGC AGACTCGGAC TCTGCGGACA GTGCCTGTAC CACCGTGCCA 
TCTCCACCCC GAGGTGTGAT CTCCAATGTG AATGAAACCT CACTGATCCT CGAGTGGAGT 
GAGCCCCGGG ACCTGGGTGG CCGGGATGAC CTCCTGTACA ATGTCATCTG CAAGAAGTGC 
CATGGGGCTG GAGGGGCCTC AGCCTGCTCA CGCTGTGATG ACAACGTGGA GTTTGTGCCT 
CGGCAGCTGG GCCTGACGGA GCGCCGGGTC CACATCAGCC ATCTGCTGGC CCACACGCGC 
TACACCTTTG AGGTGCAGGC GGTCAACGGT GTCTCGGGCA AGAGCCCTCT GCCGCCTCGT 
TATGCGGCCG TGAATATCAC CACAAACCAG GCTGCCCCGT CTGAAGTGCC CACACTACGC 
CTGCACAGCA GCTCAGGCAG CAGCCTCACC CTATCCTGGG CACCCCCAGA GCGGCCCAAC 
GGAGTCATCC TGGACTACGA GATGAAGTAC TTTGAGAAGA GCGAGGGCAT CGCCTCCACA 
GTGACCAGCC AGATGAACTC CGTGCAGCTG GACGGGCTTC GGCCTGACGC CCGCTATGTG 
GTCCAGGTCC GTGCCCGCAC AGTAGCTGGC TATGGGCAGT ACAGCCGCCC TGCCGAGTTT 
GAGACCACAA GTGAGAGAGG CTCTGGGGCC CAGCAGCTCC AGGAGCAGCT TCCCCTCATC 
GTGGGCTCCG CTACAGCTGG GCTTGTCTTC GTGGTGGCTG TCGTGGTCAT CGCTATCGTC 
TGCCTCAGGA AGCAGCGACA CGGCTCTGAT TCGGAGTACA CGGAGAAGCT GCAGCAGTAC 
ATTGCTCCTG GAATGAAGGT TTATATTGAC CCTTTTACCT ACGAGGACCC TAATGAGGCT 
GTTCGGGAGT TTGCCAAGGA GATCGACGTG TCCTGCGTCA AGATCGAGGA GGTGATCGGA 
GCTGGGGAAT TTGGGGAAGT GTGCCGTGGT CGACTGAAAC AGCCTGGCCG CCGAGAGGTG 
TTTGTGGCCA TCAAGACGCT GAAGGTGGGC TACACCGAGA GGCAGCGGCG GGACTTCCTA 
AGCGAGGCCT CCATCATGGG TCAGTTTGAT CACCCCAATA TAATCCGGCT CGAGGGCGTG 
GTCACCAAAA GTCGGCCAGT TATGATCCTC ACTGAGTTCA TGGAAAACTG CGCCCTGGAC 
TCCTTCCTCC GGCTCAACGA TGGGCAGTTC ACGGTCATCC AGCTGGTGGG CATGTTGCGG 
GGCATTGCTG CCGGCATGAA GTACCTGTCC GAGATGAACT ATGTGCACCG CGACCTGGCT 
GCTCGCAACA TCCTTGTCAA CAGCAACCTG GTCTGCAAAG TCTCAGACTT TGGCCTCTCC 
CGCTTCCTGG AGGATGACCC CTCCGATCCT ACCTACACCA GTTCCCTGGG CGGGAAGATC 
CCCATCCGCT GGACTGCCCC AGAGGCCATA GCCTATCGGA AGTTCACTTC TGCTAGTGAT 
GTCTGGAGCT ACGGAATTGT CATGTGGGAG GTCATGAGCT ATGGAGAGCG ACCCTACTGG 
GACATGAGCA ACCAGGATGT CATCAATGCC GTGGAGCAGG ATTACCGGCT GCCACCACCC 
ATGGACTGTC CCACAGCACT GCACCAGCTC ATGCTGGACT GCTGGGTGCG GGACCGGAAC 
CTCAGGCCCA AATTCTCCCA GATTGTCAAT ACCCTGGACA AGCTCATCCG CAATGCTGCC 
AGCCTCAAGG TCATTGCCAG CGCTCAGTCT GGCATGTCAC AGCCCCTCCT GGACCGCACG 
GTCCCAGATT ACACAACCTT CACGACAGTT GGTGATTGGC TGGATGCCAT CAAGATGGGG 
CGGTACAAGG AGAGCTTCGT CAGTGCGGGG TTTGCATCTT TTGACCTGGT GGCCCAGATG 
ACGGCAGAAG ACCTGCTCCG TATTGGGGTC ACCCTGGCCG GCCACCAGAA GAAGATCCTG 
AGCAGTATCC AGGACATGCG GCTGCAGATG AACCAGACGC TGCCTGTGCA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHB3 ( NM_004443.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004434.2 Homo sapiens EPH receptor B3 (EPHB3), mRNA.

Price & Availability↑

CloneID	OHu55774
Clone ID Related Accession (Same CDS sequence)	NM_004443.3 , NM_004443.4
Accession Version	NM_004443.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	ephrin type-B receptor 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112643.12, CB993133.1, BC052968.1 and X75208.1. On Nov 23, 2018 this sequence version replaced NM_004443.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052968.1, X75208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330394.3/ ENSP00000332118.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAGAG CCCGCCCGCC GCCGCCGCCG TCGCCGCCGC CGGGGCTTCT GCCGCTGCTC 
CCTCCGCTGC TGCTGCTGCC GCTGCTGCTG CTGCCCGCCG GCTGCCGGGC GCTGGAAGAG 
ACCCTCATGG ACACAAAATG GGTAACATCT GAGTTGGCGT GGACATCTCA TCCAGAAAGT 
GGGTGGGAAG AGGTGAGTGG CTACGATGAG GCCATGAATC CCATCCGCAC ATACCAGGTG 
TGTAATGTGC GCGAGTCAAG CCAGAACAAC TGGCTTCGCA CGGGGTTCAT CTGGCGGCGG 
GATGTGCAGC GGGTCTACGT GGAGCTCAAG TTCACTGTGC GTGACTGCAA CAGCATCCCC 
AACATCCCCG GCTCCTGCAA GGAGACCTTC AACCTCTTCT ACTACGAGGC TGACAGCGAT 
GTGGCCTCAG CCTCCTCCCC CTTCTGGATG GAGAACCCCT ACGTGAAAGT GGACACCATT 
GCACCCGATG AGAGCTTCTC GCGGCTGGAT GCCGGCCGTG TCAACACCAA GGTGCGCAGC 
TTTGGGCCAC TTTCCAAGGC TGGCTTCTAC CTGGCCTTCC AGGACCAGGG CGCCTGCATG 
TCGCTCATCT CCGTGCGCGC CTTCTACAAG AAGTGTGCAT CCACCACCGC AGGCTTCGCA 
CTCTTCCCCG AGACCCTCAC TGGGGCGGAG CCCACCTCGC TGGTCATTGC TCCTGGCACC 
TGCATCCCTA ACGCCGTGGA GGTGTCGGTG CCACTCAAGC TCTACTGCAA CGGCGATGGG 
GAGTGGATGG TGCCTGTGGG TGCCTGCACC TGTGCCACCG GCCATGAGCC AGCTGCCAAG 
GAGTCCCAGT GCCGCCCCTG TCCCCCTGGG AGCTACAAGG CGAAGCAGGG AGAGGGGCCC 
TGCCTCCCAT GTCCCCCCAA CAGCCGTACC ACCTCCCCAG CCGCCAGCAT CTGCACCTGC 
CACAATAACT TCTACCGTGC AGACTCGGAC TCTGCGGACA GTGCCTGTAC CACCGTGCCA 
TCTCCACCCC GAGGTGTGAT CTCCAATGTG AATGAAACCT CACTGATCCT CGAGTGGAGT 
GAGCCCCGGG ACCTGGGTGG CCGGGATGAC CTCCTGTACA ATGTCATCTG CAAGAAGTGC 
CATGGGGCTG GAGGGGCCTC AGCCTGCTCA CGCTGTGATG ACAACGTGGA GTTTGTGCCT 
CGGCAGCTGG GCCTGACGGA GCGCCGGGTC CACATCAGCC ATCTGCTGGC CCACACGCGC 
TACACCTTTG AGGTGCAGGC GGTCAACGGT GTCTCGGGCA AGAGCCCTCT GCCGCCTCGT 
TATGCGGCCG TGAATATCAC CACAAACCAG GCTGCCCCGT CTGAAGTGCC CACACTACGC 
CTGCACAGCA GCTCAGGCAG CAGCCTCACC CTATCCTGGG CACCCCCAGA GCGGCCCAAC 
GGAGTCATCC TGGACTACGA GATGAAGTAC TTTGAGAAGA GCGAGGGCAT CGCCTCCACA 
GTGACCAGCC AGATGAACTC CGTGCAGCTG GACGGGCTTC GGCCTGACGC CCGCTATGTG 
GTCCAGGTCC GTGCCCGCAC AGTAGCTGGC TATGGGCAGT ACAGCCGCCC TGCCGAGTTT 
GAGACCACAA GTGAGAGAGG CTCTGGGGCC CAGCAGCTCC AGGAGCAGCT TCCCCTCATC 
GTGGGCTCCG CTACAGCTGG GCTTGTCTTC GTGGTGGCTG TCGTGGTCAT CGCTATCGTC 
TGCCTCAGGA AGCAGCGACA CGGCTCTGAT TCGGAGTACA CGGAGAAGCT GCAGCAGTAC 
ATTGCTCCTG GAATGAAGGT TTATATTGAC CCTTTTACCT ACGAGGACCC TAATGAGGCT 
GTTCGGGAGT TTGCCAAGGA GATCGACGTG TCCTGCGTCA AGATCGAGGA GGTGATCGGA 
GCTGGGGAAT TTGGGGAAGT GTGCCGTGGT CGACTGAAAC AGCCTGGCCG CCGAGAGGTG 
TTTGTGGCCA TCAAGACGCT GAAGGTGGGC TACACCGAGA GGCAGCGGCG GGACTTCCTA 
AGCGAGGCCT CCATCATGGG TCAGTTTGAT CACCCCAATA TAATCCGGCT CGAGGGCGTG 
GTCACCAAAA GTCGGCCAGT TATGATCCTC ACTGAGTTCA TGGAAAACTG CGCCCTGGAC 
TCCTTCCTCC GGCTCAACGA TGGGCAGTTC ACGGTCATCC AGCTGGTGGG CATGTTGCGG 
GGCATTGCTG CCGGCATGAA GTACCTGTCC GAGATGAACT ATGTGCACCG CGACCTGGCT 
GCTCGCAACA TCCTTGTCAA CAGCAACCTG GTCTGCAAAG TCTCAGACTT TGGCCTCTCC 
CGCTTCCTGG AGGATGACCC CTCCGATCCT ACCTACACCA GTTCCCTGGG CGGGAAGATC 
CCCATCCGCT GGACTGCCCC AGAGGCCATA GCCTATCGGA AGTTCACTTC TGCTAGTGAT 
GTCTGGAGCT ACGGAATTGT CATGTGGGAG GTCATGAGCT ATGGAGAGCG ACCCTACTGG 
GACATGAGCA ACCAGGATGT CATCAATGCC GTGGAGCAGG ATTACCGGCT GCCACCACCC 
ATGGACTGTC CCACAGCACT GCACCAGCTC ATGCTGGACT GCTGGGTGCG GGACCGGAAC 
CTCAGGCCCA AATTCTCCCA GATTGTCAAT ACCCTGGACA AGCTCATCCG CAATGCTGCC 
AGCCTCAAGG TCATTGCCAG CGCTCAGTCT GGCATGTCAC AGCCCCTCCT GGACCGCACG 
GTCCCAGATT ACACAACCTT CACGACAGTT GGTGATTGGC TGGATGCCAT CAAGATGGGG 
CGGTACAAGG AGAGCTTCGT CAGTGCGGGG TTTGCATCTT TTGACCTGGT GGCCCAGATG 
ACGGCAGAAG ACCTGCTCCG TATTGGGGTC ACCCTGGCCG GCCACCAGAA GAAGATCCTG 
AGCAGTATCC AGGACATGCG GCTGCAGATG AACCAGACGC TGCCTGTGCA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004434.2
CDS	452..3448
Translation	MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSATAGLVFVVAVVVIAIVCLRKQRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV

Target ORF information:

RefSeq Version	NM_004443.4
Organism	Homo sapiens(human)
Definition	Homo sapiens EPH receptor B3 (EPHB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004443.4

ORF Insert Sequence:

ATGGCCAGAG CCCGCCCGCC GCCGCCGCCG TCGCCGCCGC CGGGGCTTCT GCCGCTGCTC 
CCTCCGCTGC TGCTGCTGCC GCTGCTGCTG CTGCCCGCCG GCTGCCGGGC GCTGGAAGAG 
ACCCTCATGG ACACAAAATG GGTAACATCT GAGTTGGCGT GGACATCTCA TCCAGAAAGT 
GGGTGGGAAG AGGTGAGTGG CTACGATGAG GCCATGAATC CCATCCGCAC ATACCAGGTG 
TGTAATGTGC GCGAGTCAAG CCAGAACAAC TGGCTTCGCA CGGGGTTCAT CTGGCGGCGG 
GATGTGCAGC GGGTCTACGT GGAGCTCAAG TTCACTGTGC GTGACTGCAA CAGCATCCCC 
AACATCCCCG GCTCCTGCAA GGAGACCTTC AACCTCTTCT ACTACGAGGC TGACAGCGAT 
GTGGCCTCAG CCTCCTCCCC CTTCTGGATG GAGAACCCCT ACGTGAAAGT GGACACCATT 
GCACCCGATG AGAGCTTCTC GCGGCTGGAT GCCGGCCGTG TCAACACCAA GGTGCGCAGC 
TTTGGGCCAC TTTCCAAGGC TGGCTTCTAC CTGGCCTTCC AGGACCAGGG CGCCTGCATG 
TCGCTCATCT CCGTGCGCGC CTTCTACAAG AAGTGTGCAT CCACCACCGC AGGCTTCGCA 
CTCTTCCCCG AGACCCTCAC TGGGGCGGAG CCCACCTCGC TGGTCATTGC TCCTGGCACC 
TGCATCCCTA ACGCCGTGGA GGTGTCGGTG CCACTCAAGC TCTACTGCAA CGGCGATGGG 
GAGTGGATGG TGCCTGTGGG TGCCTGCACC TGTGCCACCG GCCATGAGCC AGCTGCCAAG 
GAGTCCCAGT GCCGCCCCTG TCCCCCTGGG AGCTACAAGG CGAAGCAGGG AGAGGGGCCC 
TGCCTCCCAT GTCCCCCCAA CAGCCGTACC ACCTCCCCAG CCGCCAGCAT CTGCACCTGC 
CACAATAACT TCTACCGTGC AGACTCGGAC TCTGCGGACA GTGCCTGTAC CACCGTGCCA 
TCTCCACCCC GAGGTGTGAT CTCCAATGTG AATGAAACCT CACTGATCCT CGAGTGGAGT 
GAGCCCCGGG ACCTGGGTGG CCGGGATGAC CTCCTGTACA ATGTCATCTG CAAGAAGTGC 
CATGGGGCTG GAGGGGCCTC AGCCTGCTCA CGCTGTGATG ACAACGTGGA GTTTGTGCCT 
CGGCAGCTGG GCCTGACGGA GCGCCGGGTC CACATCAGCC ATCTGCTGGC CCACACGCGC 
TACACCTTTG AGGTGCAGGC GGTCAACGGT GTCTCGGGCA AGAGCCCTCT GCCGCCTCGT 
TATGCGGCCG TGAATATCAC CACAAACCAG GCTGCCCCGT CTGAAGTGCC CACACTACGC 
CTGCACAGCA GCTCAGGCAG CAGCCTCACC CTATCCTGGG CACCCCCAGA GCGGCCCAAC 
GGAGTCATCC TGGACTACGA GATGAAGTAC TTTGAGAAGA GCGAGGGCAT CGCCTCCACA 
GTGACCAGCC AGATGAACTC CGTGCAGCTG GACGGGCTTC GGCCTGACGC CCGCTATGTG 
GTCCAGGTCC GTGCCCGCAC AGTAGCTGGC TATGGGCAGT ACAGCCGCCC TGCCGAGTTT 
GAGACCACAA GTGAGAGAGG CTCTGGGGCC CAGCAGCTCC AGGAGCAGCT TCCCCTCATC 
GTGGGCTCCG CTACAGCTGG GCTTGTCTTC GTGGTGGCTG TCGTGGTCAT CGCTATCGTC 
TGCCTCAGGA AGCAGCGACA CGGCTCTGAT TCGGAGTACA CGGAGAAGCT GCAGCAGTAC 
ATTGCTCCTG GAATGAAGGT TTATATTGAC CCTTTTACCT ACGAGGACCC TAATGAGGCT 
GTTCGGGAGT TTGCCAAGGA GATCGACGTG TCCTGCGTCA AGATCGAGGA GGTGATCGGA 
GCTGGGGAAT TTGGGGAAGT GTGCCGTGGT CGACTGAAAC AGCCTGGCCG CCGAGAGGTG 
TTTGTGGCCA TCAAGACGCT GAAGGTGGGC TACACCGAGA GGCAGCGGCG GGACTTCCTA 
AGCGAGGCCT CCATCATGGG TCAGTTTGAT CACCCCAATA TAATCCGGCT CGAGGGCGTG 
GTCACCAAAA GTCGGCCAGT TATGATCCTC ACTGAGTTCA TGGAAAACTG CGCCCTGGAC 
TCCTTCCTCC GGCTCAACGA TGGGCAGTTC ACGGTCATCC AGCTGGTGGG CATGTTGCGG 
GGCATTGCTG CCGGCATGAA GTACCTGTCC GAGATGAACT ATGTGCACCG CGACCTGGCT 
GCTCGCAACA TCCTTGTCAA CAGCAACCTG GTCTGCAAAG TCTCAGACTT TGGCCTCTCC 
CGCTTCCTGG AGGATGACCC CTCCGATCCT ACCTACACCA GTTCCCTGGG CGGGAAGATC 
CCCATCCGCT GGACTGCCCC AGAGGCCATA GCCTATCGGA AGTTCACTTC TGCTAGTGAT 
GTCTGGAGCT ACGGAATTGT CATGTGGGAG GTCATGAGCT ATGGAGAGCG ACCCTACTGG 
GACATGAGCA ACCAGGATGT CATCAATGCC GTGGAGCAGG ATTACCGGCT GCCACCACCC 
ATGGACTGTC CCACAGCACT GCACCAGCTC ATGCTGGACT GCTGGGTGCG GGACCGGAAC 
CTCAGGCCCA AATTCTCCCA GATTGTCAAT ACCCTGGACA AGCTCATCCG CAATGCTGCC 
AGCCTCAAGG TCATTGCCAG CGCTCAGTCT GGCATGTCAC AGCCCCTCCT GGACCGCACG 
GTCCCAGATT ACACAACCTT CACGACAGTT GGTGATTGGC TGGATGCCAT CAAGATGGGG 
CGGTACAAGG AGAGCTTCGT CAGTGCGGGG TTTGCATCTT TTGACCTGGT GGCCCAGATG 
ACGGCAGAAG ACCTGCTCCG TATTGGGGTC ACCCTGGCCG GCCACCAGAA GAAGATCCTG 
AGCAGTATCC AGGACATGCG GCTGCAGATG AACCAGACGC TGCCTGTGCA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EPHB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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