HTRA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HTRA4
Entrez Gene ID	203100
Full Name	HtrA serine peptidase 4
General protein information	Preferred Names HtrA serine peptidase 4 Names serine protease HTRA4 high-temperature requirement factor A4 probable serine protease HTRA4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p11.22
Summary	This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging. [provided by RefSeq, Oct 2008].
Disorder MIM:	610700

mRNA and Protein(s)

mRNA	Protein	Name
NM_153692.4	NP_710159.1	serine protease HTRA4 precursor
XM_011544431.2	XP_011542733.1	serine protease HTRA4 isoform X1
XM_011544432.1	XP_011542734.1	serine protease HTRA4 isoform X2
NM_153692.3	NP_710159.1	serine protease HTRA4 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100334257	XP_002662051.2
	HTRA4	XP_001092132.2
Canis lupus familiaris (dog)	LOC475580	XP_005629977.1
Xenopus tropicalis (tropical clawed frog)	LOC100488607	XP_002932829.1
Homo sapiens (human)	HTRA4	NP_710159.1
Pan troglodytes (chimpanzee)	HTRA4	XP_528115.1
Bos taurus (cattle)	HTRA4	NP_001258943.1
Mus musculus (house mouse)	Htra4	NP_001074656.1
Rattus norvegicus (Norway rat)	Htra4	NP_001100791.1

Gene Ontology
Bibliography

Related articles in PubMed

Peptide selectivity between the PDZ domains of human pregnancy-related serine proteases (HtrA1, HtrA2, HtrA3, and HtrA4) can be reshaped by different halogen probes.
Sun ML, Sun LM, Wang YQ
Journal of molecular recognition : JMR(2017 Dec)

GATA3 inhibits GCM1 activity and trophoblast cell invasion.
Chiu YH, Chen H
Scientific reports621630(2016 Feb)

Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction.
Singh H, Zhao M, Chen Q, Wang Y, Li Y, Kaitu'u-Lino TJ, Tong S, Nie G
The Journal of clinical endocrinology and metabolism100(7)E936-45(2015 Jul)

Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion.
Chen YY, Chuang PY, Chen CP, Chiu YH, Lo HF, Cheong ML, Huang JY, Kuo PL, Chen H
The Journal of biological chemistry289(33)22958-68(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GATA3 knockdown elevated HtrA4 expression in BeWo and JEG-3 trophoblast cell lines and enhanced the invasion activities of both lines. This study uncovered a new GATA3 function in placenta as a negative regulator of GCM1 activity and trophoblastic invasion.
Title: GATA3 inhibits GCM1 activity and trophoblast cell invasion.

HtrA4 represents a novel placenta-specific serine protease that is altered specifically in early-onset preeclampsia with potential causal roles in endothelial dysfunction and disease development.
Title: Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction.

Conditioned media from these two cell lines after decidualization treatment suppress HtrA4-expressing JAR cell invasion in an HtrA1- or HtrA3-dependent manner.
Title: Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion.

Data suggest that increased HtrA4 (and HtrA1) in placenta tissues may be involved in onset of pre-eclampsia; elevated level of HtrA4 (but not HtrA1) in sera has potential as biomarker for pre-eclampsia.
Title: Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsia.

study reveals a novel function of GCM1 and HtrA4 in regulation of trophoblast invasion and that abnormal HrtA4 expression may contribute to shallow trophoblast invasion in preeclampsia
Title: High-temperature requirement protein A4 (HtrA4) suppresses the fusogenic activity of syncytin-1 and promotes trophoblast invasion.

The following HTRA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HTRA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12507	NM_153692.4 Latest version!	Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu77825	XM_011544431.2 Latest version!	Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu77826	XM_011544432.1 Latest version!	Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu12507	NM_153692.3	Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HTRA4 ( NM_153692.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_710159.1 Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.

Price & Availability↑

CloneID	OHu12507
Clone ID Related Accession (Same CDS sequence)	NM_153692.4 , NM_153692.3
Accession Version	NM_153692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	serine protease HTRA4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK075205.1, AC108863.4 and AY188774.1. On Nov 23, 2018 this sequence version replaced NM_153692.3. ##Evidence-Data-START## Transcript exon combination :: BC057765.1, AK075205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302495.5/ ENSP00000305919.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGGAAAGG CGTTTTCAAA TAAGAAATAT 
CTGGGTCTGC AAATGCTGTC CCTCACTGTG CCCCTTAGTG AAGAATTGAA AATGCATTAT 
CCAGATTTCC CTGATGTGAG TTCTGGGGTT TATGTATGTA AAGTGGTTGA AGGAACAGCT 
GCTCAAAGCT CTGGATTGAG AGATCACGAT GTAATTGTCA ACATAAATGG GAAACCTATT 
ACTACTACAA CTGATGTTGT TAAAGCTCTT GACAGTGATT CCCTTTCCAT GGCTGTTCTT 
CGGGGAAAAG ATAATTTGCT CCTGACAGTC ATACCTGAAA CAATCAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_710159.1
CDS	37..1467
Translation	MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQMLSLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNINGKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETIN

Target ORF information:

RefSeq Version	NM_153692.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153692.4

ORF Insert Sequence:

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGGAAAGG CGTTTTCAAA TAAGAAATAT 
CTGGGTCTGC AAATGCTGTC CCTCACTGTG CCCCTTAGTG AAGAATTGAA AATGCATTAT 
CCAGATTTCC CTGATGTGAG TTCTGGGGTT TATGTATGTA AAGTGGTTGA AGGAACAGCT 
GCTCAAAGCT CTGGATTGAG AGATCACGAT GTAATTGTCA ACATAAATGG GAAACCTATT 
ACTACTACAA CTGATGTTGT TAAAGCTCTT GACAGTGATT CCCTTTCCAT GGCTGTTCTT 
CGGGGAAAAG ATAATTTGCT CCTGACAGTC ATACCTGAAA CAATCAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTRA4 ( NM_153692.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_710159.1 Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.

Price & Availability↑

CloneID	OHu12507
Clone ID Related Accession (Same CDS sequence)	NM_153692.4 , NM_153692.3
Accession Version	NM_153692.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	serine protease HTRA4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC423042.1, AK075205.1, AC108863.4 and AY188774.1. On Oct 28, 2008 this sequence version replaced NM_153692.2. ##Evidence-Data-START## Transcript exon combination :: AK075205.1, BC057765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGGAAAGG CGTTTTCAAA TAAGAAATAT 
CTGGGTCTGC AAATGCTGTC CCTCACTGTG CCCCTTAGTG AAGAATTGAA AATGCATTAT 
CCAGATTTCC CTGATGTGAG TTCTGGGGTT TATGTATGTA AAGTGGTTGA AGGAACAGCT 
GCTCAAAGCT CTGGATTGAG AGATCACGAT GTAATTGTCA ACATAAATGG GAAACCTATT 
ACTACTACAA CTGATGTTGT TAAAGCTCTT GACAGTGATT CCCTTTCCAT GGCTGTTCTT 
CGGGGAAAAG ATAATTTGCT CCTGACAGTC ATACCTGAAA CAATCAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_710159.1
CDS	116..1546
Translation	MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQMLSLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNINGKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETIN

Target ORF information:

RefSeq Version	NM_153692.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153692.3

ORF Insert Sequence:

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGGAAAGG CGTTTTCAAA TAAGAAATAT 
CTGGGTCTGC AAATGCTGTC CCTCACTGTG CCCCTTAGTG AAGAATTGAA AATGCATTAT 
CCAGATTTCC CTGATGTGAG TTCTGGGGTT TATGTATGTA AAGTGGTTGA AGGAACAGCT 
GCTCAAAGCT CTGGATTGAG AGATCACGAT GTAATTGTCA ACATAAATGG GAAACCTATT 
ACTACTACAA CTGATGTTGT TAAAGCTCTT GACAGTGATT CCCTTTCCAT GGCTGTTCTT 
CGGGGAAAAG ATAATTTGCT CCTGACAGTC ATACCTGAAA CAATCAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTRA4 ( XM_011544431.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542733.1 Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu77825
Clone ID Related Accession (Same CDS sequence)	XM_011544431.2
Accession Version	XM_011544431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine protease HTRA4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGAGGCAA AAACCATAGC TGCACAGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542733.1
CDS	116..1258
Translation	MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKEAKTIAAQS

Target ORF information:

RefSeq Version	XM_011544431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544431.2

ORF Insert Sequence:

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG ATGGTGATGT GATTGGCGTC 
AATTCATTGA GGGTGACTGA TGGAATCTCC TTTGCAATTC CTTCAGATCG AGTTAGGCAG 
TTCTTGGCAG AATACCATGA GCACCAGATG AAAGAGGCAA AAACCATAGC TGCACAGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HTRA4 ( XM_011544432.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542734.1 Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X2, mRNA.

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CloneID	OHu77826
Clone ID Related Accession (Same CDS sequence)	XM_011544432.1
Accession Version	XM_011544432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine protease HTRA4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG TTTTTAGTCT TGACCTCTTG 
ACCTGCTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542734.1
CDS	116..1147
Translation	MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLVFSLDLLTCL

Target ORF information:

RefSeq Version	XM_011544432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HtrA serine peptidase 4 (HTRA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544432.1

ORF Insert Sequence:

ATGATTAGAC CTCAGCTGCG GACCGCGGGG CTGGGACGAT GCCTCCTGCC GGGGCTGCTG 
CTGCTCCTGG TGCCCGTCCT CTGGGCCGGG GCTGAAAAGC TACATACCCA GCCCTCCTGC 
CCCGCGGTCT GCCAGCCCAC GCGCTGCCCC GCGCTGCCCA CCTGCGCGCT GGGGACCACG 
CCGGTGTTCG ACCTGTGCCG CTGTTGCCGC GTCTGCCCCG CGGCCGAGCG TGAAGTCTGC 
GGCGGGGCGC AGGGCCAACC GTGCGCCCCG GGGCTGCAGT GCCTCCAGCC GCTGCGCCCC 
GGGTTCCCCA GCACCTGCGG TTGCCCGACG CTGGGAGGGG CCGTGTGCGG CAGCGACAGG 
CGCACCTACC CCAGCATGTG CGCGCTCCGG GCCGAAAACC GCGCCGCGCG CCGCCTGGGC 
AAGGTCCCGG CCGTGCCTGT GCAGTGGGGG AACTGCGGGG ATACAGGGAC CAGAAGCGCA 
GGCCCGCTCA GGAGGAATTA CAACTTCATC GCCGCGGTGG TGGAGAAGGT GGCGCCATCG 
GTGGTTCACG TGCAGCTGTG GGGCAGGTTA CTTCACGGCA GCAGGCTTGT TCCTGTGTAC 
AGTGGCTCTG GGTTCATAGT GTCTGAGGAC GGGCTCATTA TTACCAATGC CCATGTTGTC 
AGGAACCAGC AGTGGATTGA GGTGGTGCTC CAGAATGGGG CCCGTTATGA AGCTGTTGTC 
AAGGATATTG ACCTTAAATT GGATCTTGCG GTGATTAAGA TTGAATCAAA TGCTGAACTT 
CCTGTACTGA TGCTGGGAAG ATCATCTGAC CTTCGGGCTG GAGAGTTTGT GGTGGCTTTG 
GGCAGCCCAT TTTCTCTGCA GAACACAGCT ACTGCAGGAA TTGTCAGCAC CAAACAGCGA 
GGGGGCAAAG AACTGGGGAT GAAGGATTCA GATATGGACT ACGTCCAGAT TGATGCCACA 
ATTAATTATG GGAATTCTGG TGGTCCTCTG GTGAACTTGG TTTTTAGTCT TGACCTCTTG 
ACCTGCTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Peptide selectivity between the PDZ domains of human pregnancy-related serine proteases (HtrA1, HtrA2, HtrA3, and HtrA4) can be reshaped by different halogen probes.
Journal of molecular recognition : JMR(2017 Dec)
Sun ML,Sun LM,Wang YQ

GATA3 inhibits GCM1 activity and trophoblast cell invasion.
Scientific reports621630(2016 Feb)
Chiu YH,Chen H

Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction.
The Journal of clinical endocrinology and metabolism100(7)E936-45(2015 Jul)
Singh H,Zhao M,Chen Q,Wang Y,Li Y,Kaitu'u-Lino TJ,Tong S,Nie G

Functional antagonism between high temperature requirement protein A (HtrA) family members regulates trophoblast invasion.
The Journal of biological chemistry289(33)22958-68(2014 Aug)
Chen YY,Chuang PY,Chen CP,Chiu YH,Lo HF,Cheong ML,Huang JY,Kuo PL,Chen H

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HTRA4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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