PDE12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE12
Entrez Gene ID	201626
Full Name	phosphodiesterase 12
Synonyms	2'-PDE,2-PDE
General protein information	Preferred Names phosphodiesterase 12 Names 2',5'-phosphodiesterase 12 2'-phosphodiesterase mitochondrial deadenylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p14.3
Disorder MIM:	616519

mRNA and Protein(s)

mRNA	Protein	Name
NM_001322176.2	NP_001309105.1	2',5'-phosphodiesterase 12 isoform 2
NM_001322177.1	NP_001309106.1	2',5'-phosphodiesterase 12 isoform 3 precursor
NM_001322176.1	NP_001309105.1	2',5'-phosphodiesterase 12 isoform 2
NM_177966.6	NP_808881.3	2',5'-phosphodiesterase 12 isoform 1 precursor
NM_001322177.2	NP_001309106.1	2',5'-phosphodiesterase 12 isoform 3 precursor
NM_177966.7	NP_808881.3	2',5'-phosphodiesterase 12 isoform 1 precursor

Pathways from BioSystems

Homologies

	PDE12	XP_001099328.1
Canis lupus familiaris (dog)	PDE12	XP_541830.2
Rattus norvegicus (Norway rat)	Pde12	NP_001014020.2
Homo sapiens (human)	PDE12	NP_808881.3
Bos taurus (cattle)	PDE12	NP_001069601.1
Mus musculus (house mouse)	Pde12	NP_848783.3
Danio rerio (zebrafish)	pde12	NP_001038753.2
Drosophila melanogaster (fruit fly)	CG31759	NP_723735.2
Pan troglodytes (chimpanzee)	PDE12	XP_517033.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009079	XP_319828.3

Gene Ontology
Bibliography

Related articles in PubMed

The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of the Innate Immune Response and the Discovery of Antiviral Inhibitors.
Wood ER, Bledsoe R, Chai J, Daka P, Deng H, Ding Y, Harris-Gurley S, Kryn LH, Nartey E, Nichols J, Nolte RT, Prabhu N, Rise C, Sheahan T, Shotwell JB, Smith D, Tai V, Taylor JD, Tomberlin G, Wang L, Wisely B, You S, Xia B, Dickson H
The Journal of biological chemistry290(32)19681-96(2015 Aug)

Characterization of human phosphodiesterase 12 and identification of a novel 2'-5' oligoadenylate nuclease - The ectonucleotide pyrophosphatase/phosphodiesterase 1.
Poulsen JB, Andersen KR, Kj?r KH, Vestergaard AL, Justesen J, Martensen PM
Biochimie94(5)1098-107(2012 May)

PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria.
Rorbach J, Nicholls TJ, Minczuk M
Nucleic acids research39(17)7750-63(2011 Sep)

Human 2'-phosphodiesterase localizes to the mitochondrial matrix with a putative function in mitochondrial RNA turnover.
Poulsen JB, Andersen KR, Kj?r KH, Durand F, Faou P, Vestergaard AL, Talbo GH, Hoogenraad N, Brodersen DE, Justesen J, Martensen PM
Nucleic acids research39(9)3754-70(2011 May)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDE12 is a negative regulator of the antiviral innate immune response.
Title: The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of the Innate Immune Response and the Discovery of Antiviral Inhibitors.

2'-phosphodiesterase is a mitochondrial protein that specifically removes poly(A) extensions from mitochondrial mRNAs both in vitro and in mitochondria of cultured cells.
Title: PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria.

2'-PDE locates to the mitochondrial matrix of human cells, and comprise an active 3'-5' exoribonuclease exhibiting a preference for oligo-adenosine RNA like canonical cytoplasmic deadenylases.
Title: Human 2'-phosphodiesterase localizes to the mitochondrial matrix with a putative function in mitochondrial RNA turnover.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

crystals of phosphodiesterase 12 belonged to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 111.3, c = 192.4 A, and diffracted to 2.5 A resolution.
Title: Crystallization and preliminary X-ray crystallographic analysis of human phosphodiesterase 12.

The following PDE12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30697	NM_177966.7 Latest version!	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu77764	NM_001322176.2 Latest version!	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu100347	NM_001322177.2 Latest version!	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu77764	NM_001322176.1	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu100347	NM_001322177.1	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu30697	NM_177966.6	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE12 ( NM_177966.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_808881.3 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30697
Clone ID Related Accession (Same CDS sequence)	NM_177966.6 , NM_177966.7
Accession Version	NM_177966.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, AL831824.1 and U79276.1. On Nov 23, 2018 this sequence version replaced NM_177966.6. Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.131458.1, SRR1803613.242445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000311180.9/ ENSP00000309142.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTG GGTATATTCG CCTCATTCAA ATGGCAGTAG CCTTGGCTCA CATAAGACAT 
GTTTCATGTG ATCTGTATCC TGGCATACCA GTTATATTTT GTGGGGACTT TAATAGTACA 
CCATCAACAG GAATGTATCA TTTTGTCATC AATGGCAGCA TTCCAGAGGA TCATGAAGAC 
TGGGCTTCCA ATGGGGAGGA GGAAAGATGC AATATGTCTC TTACACATTT CTTCAAGCTG 
AAAAGTGCTT GTGGTGAACC TGCTTACACA AATTATGTTG GTGGCTTTCA TGGATGTCTA 
GATTACATTT TCATTGACTT AAATGCTTTA GAGGTTGAAC AGGTGATTCC ATTACCTAGT 
CATGAAGAAG TTACCACCCA CCAGGCCTTA CCTAGTGTTT CCCATCCCTC TGATCACATA 
GCACTTGTAT GTGATTTAAA ATGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808881.3
CDS	107..1936
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHFVINGSIPEDHEDWASNGEEERCNMSLTHFFKLKSACGEPAYTNYVGGFHGCLDYIFIDLNALEVEQVIPLPSHEEVTTHQALPSVSHPSDHIALVCDLKWK

Target ORF information:

RefSeq Version	NM_177966.7
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177966.7

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTG GGTATATTCG CCTCATTCAA ATGGCAGTAG CCTTGGCTCA CATAAGACAT 
GTTTCATGTG ATCTGTATCC TGGCATACCA GTTATATTTT GTGGGGACTT TAATAGTACA 
CCATCAACAG GAATGTATCA TTTTGTCATC AATGGCAGCA TTCCAGAGGA TCATGAAGAC 
TGGGCTTCCA ATGGGGAGGA GGAAAGATGC AATATGTCTC TTACACATTT CTTCAAGCTG 
AAAAGTGCTT GTGGTGAACC TGCTTACACA AATTATGTTG GTGGCTTTCA TGGATGTCTA 
GATTACATTT TCATTGACTT AAATGCTTTA GAGGTTGAAC AGGTGATTCC ATTACCTAGT 
CATGAAGAAG TTACCACCCA CCAGGCCTTA CCTAGTGTTT CCCATCCCTC TGATCACATA 
GCACTTGTAT GTGATTTAAA ATGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE12 ( NM_177966.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_808881.3 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30697
Clone ID Related Accession (Same CDS sequence)	NM_177966.6 , NM_177966.7
Accession Version	NM_177966.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, AL831824.1 and U79276.1. On Apr 7, 2016 this sequence version replaced NM_177966.5. Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.242445.1, SRR1803612.182283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTG GGTATATTCG CCTCATTCAA ATGGCAGTAG CCTTGGCTCA CATAAGACAT 
GTTTCATGTG ATCTGTATCC TGGCATACCA GTTATATTTT GTGGGGACTT TAATAGTACA 
CCATCAACAG GAATGTATCA TTTTGTCATC AATGGCAGCA TTCCAGAGGA TCATGAAGAC 
TGGGCTTCCA ATGGGGAGGA GGAAAGATGC AATATGTCTC TTACACATTT CTTCAAGCTG 
AAAAGTGCTT GTGGTGAACC TGCTTACACA AATTATGTTG GTGGCTTTCA TGGATGTCTA 
GATTACATTT TCATTGACTT AAATGCTTTA GAGGTTGAAC AGGTGATTCC ATTACCTAGT 
CATGAAGAAG TTACCACCCA CCAGGCCTTA CCTAGTGTTT CCCATCCCTC TGATCACATA 
GCACTTGTAT GTGATTTAAA ATGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808881.3
CDS	134..1963
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHFVINGSIPEDHEDWASNGEEERCNMSLTHFFKLKSACGEPAYTNYVGGFHGCLDYIFIDLNALEVEQVIPLPSHEEVTTHQALPSVSHPSDHIALVCDLKWK

Target ORF information:

RefSeq Version	NM_177966.6
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177966.6

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTG GGTATATTCG CCTCATTCAA ATGGCAGTAG CCTTGGCTCA CATAAGACAT 
GTTTCATGTG ATCTGTATCC TGGCATACCA GTTATATTTT GTGGGGACTT TAATAGTACA 
CCATCAACAG GAATGTATCA TTTTGTCATC AATGGCAGCA TTCCAGAGGA TCATGAAGAC 
TGGGCTTCCA ATGGGGAGGA GGAAAGATGC AATATGTCTC TTACACATTT CTTCAAGCTG 
AAAAGTGCTT GTGGTGAACC TGCTTACACA AATTATGTTG GTGGCTTTCA TGGATGTCTA 
GATTACATTT TCATTGACTT AAATGCTTTA GAGGTTGAAC AGGTGATTCC ATTACCTAGT 
CATGAAGAAG TTACCACCCA CCAGGCCTTA CCTAGTGTTT CCCATCCCTC TGATCACATA 
GCACTTGTAT GTGATTTAAA ATGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE12 ( NM_001322176.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309105.1 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu77764
Clone ID Related Accession (Same CDS sequence)	NM_001322176.2 , NM_001322176.1
Accession Version	NM_001322176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, BM713922.1 and AC114480.2. On May 31, 2019 this sequence version replaced NM_001322176.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. These differences result in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Isoform 2 may lack phospodiesterase or deadenylase activty. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.171102.1, SRR1660809.261059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGAAA ACTGCCCTTC AATTCTAATC TCACCTCAAA AACAGAATGA GGAAACTATT 
TCTGTGATCA AGAAAGCTGA AACCAAAGGC GTGGTCCATG GAATAGACCT TGACCTAGTG 
AAAGGAGCGC CTACACTCAA CTGTATCTCT GCTACTCAAA TTCAAATGCC TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309105.1
CDS	107..1663
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKENCPSILISPQKQNEETISVIKKAETKGVVHGIDLDLVKGAPTLNCISATQIQMPF

Target ORF information:

RefSeq Version	NM_001322176.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322176.2

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGAAA ACTGCCCTTC AATTCTAATC TCACCTCAAA AACAGAATGA GGAAACTATT 
TCTGTGATCA AGAAAGCTGA AACCAAAGGC GTGGTCCATG GAATAGACCT TGACCTAGTG 
AAAGGAGCGC CTACACTCAA CTGTATCTCT GCTACTCAAA TTCAAATGCC TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE12 ( NM_001322176.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309105.1 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu77764
Clone ID Related Accession (Same CDS sequence)	NM_001322176.2 , NM_001322176.1
Accession Version	NM_001322176.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, BM713922.1 and AC114480.2. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. These differences result in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Isoform 2 may lack phospodiesterase or deadenylase activty. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.261059.1, SRR1803611.171102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGAAA ACTGCCCTTC AATTCTAATC TCACCTCAAA AACAGAATGA GGAAACTATT 
TCTGTGATCA AGAAAGCTGA AACCAAAGGC GTGGTCCATG GAATAGACCT TGACCTAGTG 
AAAGGAGCGC CTACACTCAA CTGTATCTCT GCTACTCAAA TTCAAATGCC TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309105.1
CDS	134..1690
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKENCPSILISPQKQNEETISVIKKAETKGVVHGIDLDLVKGAPTLNCISATQIQMPF

Target ORF information:

RefSeq Version	NM_001322176.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322176.1

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGAAA ACTGCCCTTC AATTCTAATC TCACCTCAAA AACAGAATGA GGAAACTATT 
TCTGTGATCA AGAAAGCTGA AACCAAAGGC GTGGTCCATG GAATAGACCT TGACCTAGTG 
AAAGGAGCGC CTACACTCAA CTGTATCTCT GCTACTCAAA TTCAAATGCC TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE12 ( NM_001322177.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309106.1 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100347
Clone ID Related Accession (Same CDS sequence)	NM_001322177.1 , NM_001322177.2
Accession Version	NM_001322177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, BC034978.2 and U79276.1. Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. These differences result in a shorter protein (isoform 3) with a distinct C-terminus, compared to isoform 1. Isoform 3 may lack phospodiesterase or deadenylase activty. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.154.1, SRR1660809.164275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTA GGTTTTATTT GGTATCACAA GTGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309106.1
CDS	134..1552
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGRFYLVSQVT

Target ORF information:

RefSeq Version	NM_001322177.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322177.1

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTA GGTTTTATTT GGTATCACAA GTGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE12 ( NM_001322177.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309106.1 Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100347
Clone ID Related Accession (Same CDS sequence)	NM_001322177.1 , NM_001322177.2
Accession Version	NM_001322177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	2',5'-phosphodiesterase 12 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092418.3, BC034978.2 and U79276.1. On May 31, 2019 this sequence version replaced NM_001322177.1. Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. These differences result in a shorter protein (isoform 3) with a distinct C-terminus, compared to isoform 1. Isoform 3 may lack phospodiesterase or deadenylase activty. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.154.1, SRR1660809.164275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTA GGTTTTATTT GGTATCACAA GTGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309106.1
CDS	107..1525
Translation	MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGRFYLVSQVT

Target ORF information:

RefSeq Version	NM_001322177.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322177.2

ORF Insert Sequence:

ATGTGGAGGC TCCCAGGCGC CCGCGCCGCG CTTCGGGTGA TCCGGACGGC GGTGGAGAAG 
CTGAGCCGGG CTGAAGCGGG GAGCCAGACA GCGGCGGGAG CGATGGAGCG CGCTGTAGTG 
CGCTGCGTAC CTTCGGAACC CAAGCTGAGC CTGTCATTCG CTTTGGCTGA TGGTAGCCAC 
AAGAACATGC AGCGCGACCA GAGCGAGCCG CTGGGTCGAG TCCTCAGCCG CATCGCTACC 
AATGCCCTAA AGGGTCACGC TAAGGCGGCC GCCGCCAAGA AGAGCAGGAA GAGCCGGCCG 
AATGCTAGCG GCGGTGCGGC CTGTTCAGGG CCGGGGCCTG AGCCGGCTGT GTTCTGCGAG 
CCCGTGGTGA AGCTGTACTA CCGGGAAGAG GCAGTGGCTG AGGACGTGCT CAACGTGGAT 
GCCTGGCAAG ACGGCGCGGT GCTGCAGATC GGCGATGTTA AGTACAAGGT GGAGCGCAAC 
CCGCCCGCCT TCACCGAACT GCAGTTGCCG CGCTACATCA TGGCCGGGTT CCCTGTGTGC 
CCCAAACTCA GCCTCGAATT TGGGGATCCC GCCAGCTCCC TTTTCCGCTG GTATAAGGAA 
GCCAAGCCCG GAGCGGCGGA GCCCGAGGTC GGTGTCCCCT CGTCATTGTC TCCCTCCTCA 
CCTTCTTCTT CTTGGACTGA GACTGATGTG GAGGAGCGTG TCTACACCCC GTCCAATGCC 
GACATCGGGC TAAGGCTCAA GCTTCACTGC ACCCCAGGCG ATGGGCAGCG CTTTGGGCAC 
AGCCGGGAGT TGGAAAGTGT GTGTGTGGTA GAGGCTGGGC CTGGCACCTG CACTTTTGAC 
CACCGGCATC TCTACACGAA GAAGGTGACT GAGGACGCTC TCATCCGCAC TGTCTCTTAC 
AACATCCTGG CAGACACGTA CGCGCAGACT GAGTTCTCGC GAACGGTTCT GTACCCATAC 
TGTGCCCCCT ACGCCCTGGA GCTCGACTAC CGCCAGAACC TTATCCAGAA GGAACTCACC 
GGCTACAACG CCGATGTCAT CTGTTTGCAG GAGGTTGACC GCGCAGTGTT TTCTGACAGC 
TTGGTACCCG CCCTAGAGGC CTTCGGGCTC GAGGGGGTGT TTCGAATCAA GCAGCACGAA 
GGCCTGGCCA CTTTCTACCG AAAGTCTAAG TTCAGCCTTC TTAGCCAGCA TGACATTTCA 
TTCTACGAAG CCCTCGAGTC CGACCCACTT CACAAAGAAC TGCTGGAGAA ACTAGTTTTG 
TACCCATCAG CGCAGGAGAA GGTGCTCCAG AGATCTTCTG TTCTTCAGGT TTCAGTTCTT 
CAGTCTACAA AGGACTCTTC TAAAAGGATA TGTGTTGCTA ATACCCATCT TTACTGGCAT 
CCTAAAGGTA GGTTTTATTT GGTATCACAA GTGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of the Innate Immune Response and the Discovery of Antiviral Inhibitors.
The Journal of biological chemistry290(32)19681-96(2015 Aug)
Wood ER,Bledsoe R,Chai J,Daka P,Deng H,Ding Y,Harris-Gurley S,Kryn LH,Nartey E,Nichols J,Nolte RT,Prabhu N,Rise C,Sheahan T,Shotwell JB,Smith D,Tai V,Taylor JD,Tomberlin G,Wang L,Wisely B,You S,Xia B,Dickson H

Characterization of human phosphodiesterase 12 and identification of a novel 2'-5' oligoadenylate nuclease - The ectonucleotide pyrophosphatase/phosphodiesterase 1.
Biochimie94(5)1098-107(2012 May)
Poulsen JB,Andersen KR,Kj?r KH,Vestergaard AL,Justesen J,Martensen PM

PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria.
Nucleic acids research39(17)7750-63(2011 Sep)
Rorbach J,Nicholls TJ,Minczuk M

Human 2'-phosphodiesterase localizes to the mitochondrial matrix with a putative function in mitochondrial RNA turnover.
Nucleic acids research39(9)3754-70(2011 May)
Poulsen JB,Andersen KR,Kj?r KH,Durand F,Faou P,Vestergaard AL,Talbo GH,Hoogenraad N,Brodersen DE,Justesen J,Martensen PM

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

PDE12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報