STT3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	STT3B
Entrez Gene ID	201595
Full Name	STT3B, catalytic subunit of the oligosaccharyltransferase complex
Synonyms	CDG1X,SIMP,STT3-B
General protein information	Preferred Names STT3B, catalytic subunit of the oligosaccharyltransferase complex Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B STT3, subunit of the oligosaccharyltransferase complex, homolog B STT3B, subunit of the oligosaccharyltransferase complex (catalytic) dolichyl-diphosphooligosaccharide protein glycotransferase homolog of yeast STT3 oligosaccharyl transferase subunit STT3B source of immunodominant MHC-associated peptides homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p23
Summary	The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014].
Disorder MIM:	608605
Disorder Html:	?Congenital disorder of glycosylation, type Ix, 615597 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017005857.1	XP_016861346.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1
XM_017005859.1	XP_016861348.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X3
XM_017005858.1	XP_016861347.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X2
XM_011533465.1	XP_011531767.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X4
NM_178862.2	NP_849193.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
NM_178862.3	NP_849193.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
XM_017005857.2	XP_016861346.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1
XM_017005859.2	XP_016861348.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	STT3B	NP_001091039.1
Saccharomyces cerevisiae (baker's yeast)	STT3	NP_011493.1
Schizosaccharomyces pombe (fission yeast)	stt3	NP_595148.1
Magnaporthe oryzae (rice blast fungus)	MGG_04773	XP_003713725.1
Neurospora crassa	NCU10497	XP_001728408.1
Pan troglodytes (chimpanzee)	STT3B	XP_001166939.1
Rattus norvegicus (Norway rat)	Stt3b	NP_001164010.1
Gallus gallus (chicken)	STT3B	NP_001026684.1
Caenorhabditis elegans (roundworm)	stt-3	NP_498362.1
Kluyveromyces lactis	KLLA0C11627g	XP_452719.1
Eremothecium gossypii	AGOS_AFR491W	NP_986038.1
Homo sapiens (human)	STT3B	NP_849193.1
	STT3B	XP_001096091.1
Canis lupus familiaris (dog)	STT3B	XP_542747.2
Mus musculus (house mouse)	Stt3b	NP_077184.2
Drosophila melanogaster (fruit fly)	OstStt3	NP_524494.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002396	XP_312559.4
Xenopus tropicalis (tropical clawed frog)	stt3b	XP_002941861.2

Gene Ontology
Bibliography

Related articles in PubMed

Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation.
Kitajima T, Xue W, Liu YS, Wang CD, Liu SS, Fujita M, Gao XD
The FEBS journal(2017 Dec)

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.
Lin DL, Cherepanova NA, Bozzacco L, MacDonald MR, Gilmore R, Tai AW
mBio8(4)(2017 Jul)

ZMPSTE24 defends against influenza and other pathogenic viruses.
Fu B, Wang L, Li S, Dorf ME
The Journal of experimental medicine214(4)919-929(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

STT3B was significantly upregulated in hip osteoarthritis with affected versus intact cartilage, particularly in the analysis of hypertrophic and normotrophic compared with atrophic bone remodelling pattern.
Title: Radiographic endophenotyping in hip osteoarthritis improves the precision of genetic association analysis.

Post-translational modification of cotranslationally skipped sites by STT3B is hindered by the middle X residue, resulting in hypoglycosylation of consensus sites containing large hydrophobic and negatively charged side chains.
Title: The middle X residue influences cotranslational N-glycosylation consensus site skipping.

Results show homozygous mutation in STT3A and in STT3B causes congenital disorders of glycosylation.
Title: Mutations in STT3A and STT3B cause two congenital disorders of glycosylation.

Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST.
Title: Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST.

Data show that prolonged transthyretin (TTR) unfolding induces externalization of cryptic N-glycosylation site and triggers STT3B-dependent posttranslational N-glycosylation.
Title: STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein.

The following STT3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STT3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu93871	XM_017005858.1 Latest version!	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu93872	XM_017005859.2 Latest version!	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu28309	NM_178862.3 Latest version!	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$580.30 $829.00
OHu77754	XM_011533465.1 Latest version!	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu93870	XM_017005857.2 Latest version!	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu28309	NM_178862.2	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$580.30 $829.00
OHu93870	XM_017005857.1	Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu93872	XM_017005859.1	Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STT3B ( XM_017005858.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861347.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu93871
Clone ID Related Accession (Same CDS sequence)	XM_017005858.1
Accession Version	XM_017005858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGACTACCAA AAGGAAGCGT 
GGCTACATTA AAAATAAGCT GGTTTTTAAG AAAGGCAAGA AAATATCTAA GAAGACTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861347.1
CDS	258..2300
Translation	MITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV

Target ORF information:

RefSeq Version	XM_017005858.1
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005858.1

ORF Insert Sequence:

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGACTACCAA AAGGAAGCGT 
GGCTACATTA AAAATAAGCT GGTTTTTAAG AAAGGCAAGA AAATATCTAA GAAGACTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( XM_017005859.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861348.1 Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu93872
Clone ID Related Accession (Same CDS sequence)	XM_017005859.1 , XM_017005859.2
Accession Version	XM_017005859.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGGTGGGTGC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861348.1
CDS	331..2307
Translation	MITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKVGAK

Target ORF information:

RefSeq Version	XM_017005859.1
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005859.1

ORF Insert Sequence:

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGGTGGGTGC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( XM_017005859.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861348.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X3, mRNA.

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CloneID	OHu93872
Clone ID Related Accession (Same CDS sequence)	XM_017005859.1 , XM_017005859.2
Accession Version	XM_017005859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGGTGGGTGC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861348.1
CDS	331..2307
Translation	MITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKVGAK

Target ORF information:

RefSeq Version	XM_017005859.2
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005859.2

ORF Insert Sequence:

ATGATAACCG CTGGCCTTAT TCATTGGATT TTAAATACAT TGAACATAAC TGTTCACATA 
AGAGACGTAT GTGTGTTCCT TGCACCAACT TTTAGCGGCC TTACATCTAT ATCTACTTTC 
CTGCTTACAA GAGAACTTTG GAACCAAGGA GCAGGACTTT TAGCTGCTTG TTTTATTGCT 
ATTGTACCAG GCTACATATC TCGGTCAGTA GCTGGATCCT TTGATAATGA AGGCATTGCT 
ATTTTTGCAC TTCAGTTCAC ATACTATTTA TGGGTAAAAT CTGTAAAAAC TGGGTCAGTT 
TTTTGGACAA TGTGCTGCTG CTTATCCTAT TTCTATATGG TCTCTGCTTG GGGTGGTTAT 
GTATTTATCA TCAATCTTAT TCCACTGCAT GTATTTGTGT TGTTACTGAT GCAGAGATAC 
AGCAAAAGAG TCTACATAGC ATATAGCACT TTCTACATTG TGGGTTTAAT ATTATCAATG 
CAGATACCTT TTGTGGGATT CCAGCCAATC AGAACAAGTG AACACATGGC AGCTGCAGGT 
GTCTTTGCAT TGCTGCAAGC TTATGCTTTC TTGCAGTATC TGAGAGACCG ATTAACAAAA 
CAAGAGTTCC AGACCCTTTT CTTTTTGGGT GTATCACTAG CTGCAGGTGC TGTGTTCCTT 
AGTGTCATCT ATTTGACTTA TACAGGTTAC ATTGCACCAT GGAGTGGCAG GTTTTATTCA 
TTGTGGGATA CTGGGTATGC AAAAATACAC ATTCCAATTA TTGCATCAGT GTCTGAGCAT 
CAACCTACGA CTTGGGTGTC TTTCTTCTTT GATCTACATA TTCTTGTATG TACCTTCCCA 
GCAGGCCTTT GGTTCTGCAT CAAAAATATC AACGATGAAA GAGTATTTGT TGCTCTATAT 
GCAATCAGTG CTGTCTACTT TGCTGGAGTG ATGGTGCGAC TGATGTTGAC TTTGACTCCA 
GTCGTGTGTA TGCTGTCTGC AATTGCCTTT TCAAATGTTT TTGAGCACTA TTTGGGGGAT 
GACATGAAAA GGGAAAATCC ACCTGTGGAG GACAGCAGTG ATGAGGATGA CAAAAGAAAC 
CAAGGAAATT TGTATGATAA GGCAGGTAAA GTGAGGAAAC ATGCAACTGA ACAGGAAAAA 
ACTGAAGAGG GATTAGGCCC TAATATAAAA AGCATTGTCA CCATGTTGAT GCTGATGCTA 
TTGATGATGT TTGCTGTCCA CTGTACCTGG GTCACAAGCA ATGCCTACTC TAGTCCAAGT 
GTAGTCCTGG CCTCATACAA TCATGATGGC ACCAGGAATA TCTTAGATGA TTTTAGAGAA 
GCTTACTTTT GGCTAAGGCA AAATACAGAT GAACATGCAC GAGTAATGTC TTGGTGGGAT 
TATGGCTATC AGATAGCTGG AATGGCTAAT AGAACTACGT TGGTGGATAA TAACACCTGG 
AATAACAGCC ACATAGCACT GGTGGGAAAA GCTATGTCTT CTAATGAAAC AGCAGCCTAT 
AAAATCATGA GGACTCTAGA TGTAGATTAT GTTTTGGTTA TTTTTGGAGG GGTTATTGGC 
TATTCTGGTG ATGATATCAA CAAATTTCTC TGGATGGTTA GGATAGCTGA AGGAGAACAT 
CCCAAAGACA TTCGGGAAAG TGACTATTTT ACCCCACAGG GAGAATTCCG TGTAGACAAA 
GCAGGATCCC CTACTTTGTT GAATTGCCTT ATGTATAAAA TGTCATACTA CAGATTTGGA 
GAAATGCAGC TGGATTTTCG TACACCCCCA GGTTTTGACC GAACACGTAA TGCTGAGATT 
GGAAATAAGG ACATTAAATT CAAACATTTG GAAGAAGCCT TTACATCAGA ACACTGGCTT 
GTTAGGATAT ATAAAGTAAA AGCACCTGAT AACAGGGAGA CATTAGATCA CAAACCTCGA 
GTCACCAACA TTTTCCCAAA ACAGAAGTAT TTGTCAAAGA AGGTGGGTGC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( NM_178862.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_849193.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.

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CloneID	OHu28309
Clone ID Related Accession (Same CDS sequence)	NM_178862.2 , NM_178862.3
Accession Version	NM_178862.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY039873.1, HY015137.1, AY074880.1, AK075380.1, AC104643.2 and AI914841.1. This sequence is a reference standard in the RefSeqGene project. On Jun 19, 2014 this sequence version replaced NM_178862.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.174105.1, SRR1660803.8167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
ACTACCAAAA GGAAGCGTGG CTACATTAAA AATAAGCTGG TTTTTAAGAA AGGCAAGAAA 
ATATCTAAGA AGACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849193.1
CDS	499..2979
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV

Target ORF information:

RefSeq Version	NM_178862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178862.2

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
ACTACCAAAA GGAAGCGTGG CTACATTAAA AATAAGCTGG TTTTTAAGAA AGGCAAGAAA 
ATATCTAAGA AGACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( NM_178862.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_849193.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.

Price & Availability↑

CloneID	OHu28309
Clone ID Related Accession (Same CDS sequence)	NM_178862.2 , NM_178862.3
Accession Version	NM_178862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY039873.1, HY015137.1, AY074880.1, AK075380.1, AC104643.2 and AI914841.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_178862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8167.1, SRR1803616.86495.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000295770.4/ ENSP00000295770.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
ACTACCAAAA GGAAGCGTGG CTACATTAAA AATAAGCTGG TTTTTAAGAA AGGCAAGAAA 
ATATCTAAGA AGACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849193.1
CDS	75..2555
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV

Target ORF information:

RefSeq Version	NM_178862.3
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178862.3

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
ACTACCAAAA GGAAGCGTGG CTACATTAAA AATAAGCTGG TTTTTAAGAA AGGCAAGAAA 
ATATCTAAGA AGACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( XM_011533465.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531767.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X4, mRNA.

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CloneID	OHu77754
Clone ID Related Accession (Same CDS sequence)	XM_011533465.1
Accession Version	XM_011533465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGGTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531767.1
CDS	362..1696
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFGA

Target ORF information:

RefSeq Version	XM_011533465.1
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533465.1

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGGTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( XM_017005857.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861346.1 Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu93870
Clone ID Related Accession (Same CDS sequence)	XM_017005857.1 , XM_017005857.2
Accession Version	XM_017005857.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
GTGGGTGCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861346.1
CDS	362..2776
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKVGAK

Target ORF information:

RefSeq Version	XM_017005857.1
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3B, catalytic subunit of the oligosaccharyltransferase complex (STT3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005857.1

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
GTGGGTGCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STT3B ( XM_017005857.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861346.1 Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X1, mRNA.

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CloneID	OHu93870
Clone ID Related Accession (Same CDS sequence)	XM_017005857.1 , XM_017005857.2
Accession Version	XM_017005857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
GTGGGTGCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861346.1
CDS	362..2776
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKVGAK

Target ORF information:

RefSeq Version	XM_017005857.2
Organism	Homo sapiens(human)
Definition	Homo sapiens STT3 oligosaccharyltransferase complex catalytic subunit B (STT3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005857.2

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CCCTCAACTC GTCCCCGTGG 
AGTGGCCTCA TGGCCCTGGG AAACAGCCGG CACGGCCACC ACGGGCCCGG GGCCCAGTGC 
GCGCACAAGG CGGCGGGCGG CGCGGCGCCG CCGAAGCCGG CCCCGGCGGG GCTGTCCGGG 
GGGCTGTCGC AGCCGGCTGG GTGGCAGTCG CTTCTCTCCT TCACCATCCT CTTCCTGGCC 
TGGCTTGCCG GCTTCAGCTC GCGCCTCTTC GCCGTCATCC GCTTCGAAAG CATCATCCAC 
GAGTTCGACC CGTGGTTTAA CTATAGATCA ACACATCATC TTGCATCTCA TGGGTTCTAT 
GAATTTTTAA ATTGGTTTGA TGAAAGAGCA TGGTATCCAC TAGGAAGAAT AGTAGGTGGT 
ACTGTTTACC CAGGGTTGAT GATAACCGCT GGCCTTATTC ATTGGATTTT AAATACATTG 
AACATAACTG TTCACATAAG AGACGTATGT GTGTTCCTTG CACCAACTTT TAGCGGCCTT 
ACATCTATAT CTACTTTCCT GCTTACAAGA GAACTTTGGA ACCAAGGAGC AGGACTTTTA 
GCTGCTTGTT TTATTGCTAT TGTACCAGGC TACATATCTC GGTCAGTAGC TGGATCCTTT 
GATAATGAAG GCATTGCTAT TTTTGCACTT CAGTTCACAT ACTATTTATG GGTAAAATCT 
GTAAAAACTG GGTCAGTTTT TTGGACAATG TGCTGCTGCT TATCCTATTT CTATATGGTC 
TCTGCTTGGG GTGGTTATGT ATTTATCATC AATCTTATTC CACTGCATGT ATTTGTGTTG 
TTACTGATGC AGAGATACAG CAAAAGAGTC TACATAGCAT ATAGCACTTT CTACATTGTG 
GGTTTAATAT TATCAATGCA GATACCTTTT GTGGGATTCC AGCCAATCAG AACAAGTGAA 
CACATGGCAG CTGCAGGTGT CTTTGCATTG CTGCAAGCTT ATGCTTTCTT GCAGTATCTG 
AGAGACCGAT TAACAAAACA AGAGTTCCAG ACCCTTTTCT TTTTGGGTGT ATCACTAGCT 
GCAGGTGCTG TGTTCCTTAG TGTCATCTAT TTGACTTATA CAGGTTACAT TGCACCATGG 
AGTGGCAGGT TTTATTCATT GTGGGATACT GGGTATGCAA AAATACACAT TCCAATTATT 
GCATCAGTGT CTGAGCATCA ACCTACGACT TGGGTGTCTT TCTTCTTTGA TCTACATATT 
CTTGTATGTA CCTTCCCAGC AGGCCTTTGG TTCTGCATCA AAAATATCAA CGATGAAAGA 
GTATTTGTTG CTCTATATGC AATCAGTGCT GTCTACTTTG CTGGAGTGAT GGTGCGACTG 
ATGTTGACTT TGACTCCAGT CGTGTGTATG CTGTCTGCAA TTGCCTTTTC AAATGTTTTT 
GAGCACTATT TGGGGGATGA CATGAAAAGG GAAAATCCAC CTGTGGAGGA CAGCAGTGAT 
GAGGATGACA AAAGAAACCA AGGAAATTTG TATGATAAGG CAGGTAAAGT GAGGAAACAT 
GCAACTGAAC AGGAAAAAAC TGAAGAGGGA TTAGGCCCTA ATATAAAAAG CATTGTCACC 
ATGTTGATGC TGATGCTATT GATGATGTTT GCTGTCCACT GTACCTGGGT CACAAGCAAT 
GCCTACTCTA GTCCAAGTGT AGTCCTGGCC TCATACAATC ATGATGGCAC CAGGAATATC 
TTAGATGATT TTAGAGAAGC TTACTTTTGG CTAAGGCAAA ATACAGATGA ACATGCACGA 
GTAATGTCTT GGTGGGATTA TGGCTATCAG ATAGCTGGAA TGGCTAATAG AACTACGTTG 
GTGGATAATA ACACCTGGAA TAACAGCCAC ATAGCACTGG TGGGAAAAGC TATGTCTTCT 
AATGAAACAG CAGCCTATAA AATCATGAGG ACTCTAGATG TAGATTATGT TTTGGTTATT 
TTTGGAGGGG TTATTGGCTA TTCTGGTGAT GATATCAACA AATTTCTCTG GATGGTTAGG 
ATAGCTGAAG GAGAACATCC CAAAGACATT CGGGAAAGTG ACTATTTTAC CCCACAGGGA 
GAATTCCGTG TAGACAAAGC AGGATCCCCT ACTTTGTTGA ATTGCCTTAT GTATAAAATG 
TCATACTACA GATTTGGAGA AATGCAGCTG GATTTTCGTA CACCCCCAGG TTTTGACCGA 
ACACGTAATG CTGAGATTGG AAATAAGGAC ATTAAATTCA AACATTTGGA AGAAGCCTTT 
ACATCAGAAC ACTGGCTTGT TAGGATATAT AAAGTAAAAG CACCTGATAA CAGGGAGACA 
TTAGATCACA AACCTCGAGT CACCAACATT TTCCCAAAAC AGAAGTATTT GTCAAAGAAG 
GTGGGTGCCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation.
The FEBS journal(2017 Dec)
Kitajima T,Xue W,Liu YS,Wang CD,Liu SS,Fujita M,Gao XD

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.
mBio8(4)(2017 Jul)
Lin DL,Cherepanova NA,Bozzacco L,MacDonald MR,Gilmore R,Tai AW

ZMPSTE24 defends against influenza and other pathogenic viruses.
The Journal of experimental medicine214(4)919-929(2017 Apr)
Fu B,Wang L,Li S,Dorf ME

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STT3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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