EDN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDN2
Entrez Gene ID	1907
Full Name	endothelin 2
Synonyms	ET-2,ET2,PPET2
General protein information	Preferred Names endothelin 2 Names endothelin-2 preproendothelin 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.2
Summary	This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].
Disorder MIM:	131241

mRNA and Protein(s)

mRNA	Protein	Name
NM_001956.5	NP_001947.1	endothelin-2 isoform 1 preproprotein
NM_001302269.1	NP_001289198.1	endothelin-2 isoform 2 preproprotein
NM_001956.4	NP_001947.1	endothelin-2 isoform 1 preproprotein
XM_017000512.1	XP_016856001.1	endothelin-2 isoform X1
NM_001302269.2	NP_001289198.1	endothelin-2 isoform 2 preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EDN2	XP_524683.2
Canis lupus familiaris (dog)	EDN2	NP_001003002.1
Bos taurus (cattle)	EDN2	NP_783645.1
Rattus norvegicus (Norway rat)	Edn2	NP_036681.1
	EDN2	XP_001084957.1
Gallus gallus (chicken)	EDN2	NP_001185584.1
Xenopus tropicalis (tropical clawed frog)	edn2	XP_002939400.2
Homo sapiens (human)	EDN2	NP_001947.1
Mus musculus (house mouse)	Edn2	NP_031928.2

Gene Ontology
Bibliography

Related articles in PubMed

The comparison of traditional and modified harvesting techniques of left ?nternal mammary artery regarding endothelin-1/2/3 expression and free flow capacity.
Buyukates M, Barut F, Aktunc E
The Journal of cardiovascular surgery56(6)913-8(2015 Dec)

Loss of function of endothelin-2 leads to reduced ovulation and CL formation.
Cacioppo JA, Oh SW, Kim HY, Cho J, Lin PC, Yanagisawa M, Ko C
PloS one9(4)e96115(2014)

Endothelin-2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis.
Rattner A, Yu H, Williams J, Smallwood PM, Nathans J
Proceedings of the National Academy of Sciences of the United States of America110(40)E3830-9(2013 Oct)

Identification of endothelin 2 as an inflammatory factor that promotes central nervous system remyelination.
Yuen TJ, Johnson KR, Miron VE, Zhao C, Quandt J, Harrisingh MC, Swire M, Williams A, McFarland HF, Franklin RJ, Ffrench-Constant C
Brain : a journal of neurology136(Pt 4)1035-47(2013 Apr)

Expression of endothelin-2 (ET-2) gene in a human renal adenocarcinoma cell line: purification and cDNA cloning of ET-2.
Onda H, Ohkubo S, Kosaka T, Yasuhara T, Ogi K, Hosoya M, Matsumoto H, Suzuki N, Kitada C, Ishibashi Y
Journal of cardiovascular pharmacology17 Suppl 7S39-43(1991)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EDN2 expression in left internal mammary arteries depends on tissue harvesting technique.
Title: The comparison of traditional and modified harvesting techniques of left ?nternal mammary artery regarding endothelin-1/2/3 expression and free flow capacity.

Overexpression of Edn2 in the mouse retina causes an excess of tip cells and inhibits vascular growth. Overexpression of Edn2 in the brain and skin had no effect, but vascular development was inhibited in midgestation mouse embryos.
Title: Endothelin-2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis.

Endothelin 2 and endothelin receptor type B may be a regenerative pathway for central nervous system myelination and remyelination.
Title: Identification of endothelin 2 as an inflammatory factor that promotes central nervous system remyelination.

Studies revealed hypermethylation of EDN2 and EDN3 genes in human primary colon cancers and in a panel of human colon cancer cell lines. Epigenetic inactivation of ET-2 and ET-3 occurs frequently in both rat and human colon cancers.
Title: Epigenetic inactivation of endothelin-2 and endothelin-3 in colon cancer.

plasma levels of big ET-2, and big ET-3 are markedly increased in patients with end stage renal disease on hemodialysis
Title: Increased plasma levels of big-endothelin-2 and big-endothelin-3 in patients with end-stage renal disease.

The following EDN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16415	NM_001956.5 Latest version!	Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu57425	NM_001302269.2 Latest version!	Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu83260	XM_017000512.1 Latest version!	Homo sapiens endothelin 2 (EDN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu16415	NM_001956.4	Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu57425	NM_001302269.1	Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDN2 ( NM_001956.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001947.1 Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16415
Clone ID Related Accession (Same CDS sequence)	NM_001956.5 , NM_001956.4
Accession Version	NM_001956.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	endothelin-2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY177176.1, BC034393.1 and AW452560.1. On Nov 23, 2018 this sequence version replaced NM_001956.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034393.1, M65199.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372587.5/ ENSP00000361668.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGACTGA AGCCGGGGCA 
GTCCCAAGCC GGAAGTCCCC TGCAGACGTG TTCCAGACTG GCAAGACAGG GGCCACTACA 
GGAGAGCTTC TCCAAAGGCT GAGGGACATT TCCACAGTCA AGAGCCTCTT TGCCAAGCGA 
CAACAGGAGG CCATGCGGGA GCCTCGGTCC ACACATTCCA GGTGGAGGAA GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001947.1
CDS	83..619
Translation	MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSARDPACATFCLRRPWTEAGAVPSRKSPADVFQTGKTGATTGELLQRLRDISTVKSLFAKRQQEAMREPRSTHSRWRKR

Target ORF information:

RefSeq Version	NM_001956.5
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001956.5

ORF Insert Sequence:

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGACTGA AGCCGGGGCA 
GTCCCAAGCC GGAAGTCCCC TGCAGACGTG TTCCAGACTG GCAAGACAGG GGCCACTACA 
GGAGAGCTTC TCCAAAGGCT GAGGGACATT TCCACAGTCA AGAGCCTCTT TGCCAAGCGA 
CAACAGGAGG CCATGCGGGA GCCTCGGTCC ACACATTCCA GGTGGAGGAA GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN2 ( NM_001956.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001947.1 Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16415
Clone ID Related Accession (Same CDS sequence)	NM_001956.5 , NM_001956.4
Accession Version	NM_001956.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	endothelin-2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY177176.1, BC034393.1 and AW452560.1. On Oct 22, 2014 this sequence version replaced NM_001956.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034393.1, M65199.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGACTGA AGCCGGGGCA 
GTCCCAAGCC GGAAGTCCCC TGCAGACGTG TTCCAGACTG GCAAGACAGG GGCCACTACA 
GGAGAGCTTC TCCAAAGGCT GAGGGACATT TCCACAGTCA AGAGCCTCTT TGCCAAGCGA 
CAACAGGAGG CCATGCGGGA GCCTCGGTCC ACACATTCCA GGTGGAGGAA GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001947.1
CDS	83..619
Translation	MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSARDPACATFCLRRPWTEAGAVPSRKSPADVFQTGKTGATTGELLQRLRDISTVKSLFAKRQQEAMREPRSTHSRWRKR

Target ORF information:

RefSeq Version	NM_001956.4
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin 2 (EDN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001956.4

ORF Insert Sequence:

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGACTGA AGCCGGGGCA 
GTCCCAAGCC GGAAGTCCCC TGCAGACGTG TTCCAGACTG GCAAGACAGG GGCCACTACA 
GGAGAGCTTC TCCAAAGGCT GAGGGACATT TCCACAGTCA AGAGCCTCTT TGCCAAGCGA 
CAACAGGAGG CCATGCGGGA GCCTCGGTCC ACACATTCCA GGTGGAGGAA GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN2 ( NM_001302269.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289198.1 Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu57425
Clone ID Related Accession (Same CDS sequence)	NM_001302269.1 , NM_001302269.2
Accession Version	NM_001302269.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	endothelin-2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY177176.1, BC034393.1 and AW452560.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY177176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGGACAT TTCCACAGTC 
AAGAGCCTCT TTGCCAAGCG ACAACAGGAG GCCATGCGGG AGCCTCGGTC CACACATTCC 
AGGTGGAGGA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289198.1
CDS	83..520
Translation	MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSARDPACATFCLRRPWDISTVKSLFAKRQQEAMREPRSTHSRWRKR

Target ORF information:

RefSeq Version	NM_001302269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302269.1

ORF Insert Sequence:

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGGACAT TTCCACAGTC 
AAGAGCCTCT TTGCCAAGCG ACAACAGGAG GCCATGCGGG AGCCTCGGTC CACACATTCC 
AGGTGGAGGA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN2 ( NM_001302269.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289198.1 Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu57425
Clone ID Related Accession (Same CDS sequence)	NM_001302269.1 , NM_001302269.2
Accession Version	NM_001302269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	endothelin-2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY177176.1, BC034393.1 and AW452560.1. On May 31, 2019 this sequence version replaced NM_001302269.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY177176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGGACAT TTCCACAGTC 
AAGAGCCTCT TTGCCAAGCG ACAACAGGAG GCCATGCGGG AGCCTCGGTC CACACATTCC 
AGGTGGAGGA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289198.1
CDS	83..520
Translation	MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSARDPACATFCLRRPWDISTVKSLFAKRQQEAMREPRSTHSRWRKR

Target ORF information:

RefSeq Version	NM_001302269.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin 2 (EDN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302269.2

ORF Insert Sequence:

ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT 
GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA 
GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC 
TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC 
CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT 
GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGGACAT TTCCACAGTC 
AAGAGCCTCT TTGCCAAGCG ACAACAGGAG GCCATGCGGG AGCCTCGGTC CACACATTCC 
AGGTGGAGGA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDN2 ( XM_017000512.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856001.1 Homo sapiens endothelin 2 (EDN2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83260
Clone ID Related Accession (Same CDS sequence)	XM_017000512.1
Accession Version	XM_017000512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endothelin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTATC TGCTCAGCTT AATAGCAGGA CGCTGGCAAC AGGCACTCCC TGCTCCAGTC 
CAGCCTGCGC GCTCCACCGC CGCTATGGTC TCCGTGCCTA CCACCTGGTG CTCCGTTGCG 
CTAGCCCTGC TCGTGGCCCT GCATGAAGGG AAGGGCCAGG CTGCTGCCAC CCTGGAGCAG 
CCAGCGTCCT CATCTCATGC CCAAGGCACC CACCTTCGGC TTCGCCGTTG CTCCTGCAGC 
TCCTGGCTCG ACAAGGAGTG CGTCTACTTC TGCCACTTGG ACATCATCTG GGTGAACACT 
CCTGAGGACA TTTCCACAGT CAAGAGCCTC TTTGCCAAGC GACAACAGGA GGCCATGCGG 
GAGCCTCGGT CCACACATTC CAGGTGGAGG AAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856001.1
CDS	14..412
Translation	MSYLLSLIAGRWQQALPAPVQPARSTAAMVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEDISTVKSLFAKRQQEAMREPRSTHSRWRKR

Target ORF information:

RefSeq Version	XM_017000512.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin 2 (EDN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000512.1

ORF Insert Sequence:

ATGAGCTATC TGCTCAGCTT AATAGCAGGA CGCTGGCAAC AGGCACTCCC TGCTCCAGTC 
CAGCCTGCGC GCTCCACCGC CGCTATGGTC TCCGTGCCTA CCACCTGGTG CTCCGTTGCG 
CTAGCCCTGC TCGTGGCCCT GCATGAAGGG AAGGGCCAGG CTGCTGCCAC CCTGGAGCAG 
CCAGCGTCCT CATCTCATGC CCAAGGCACC CACCTTCGGC TTCGCCGTTG CTCCTGCAGC 
TCCTGGCTCG ACAAGGAGTG CGTCTACTTC TGCCACTTGG ACATCATCTG GGTGAACACT 
CCTGAGGACA TTTCCACAGT CAAGAGCCTC TTTGCCAAGC GACAACAGGA GGCCATGCGG 
GAGCCTCGGT CCACACATTC CAGGTGGAGG AAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The comparison of traditional and modified harvesting techniques of left ?nternal mammary artery regarding endothelin-1/2/3 expression and free flow capacity.
The Journal of cardiovascular surgery56(6)913-8(2015 Dec)
Buyukates M,Barut F,Aktunc E

Loss of function of endothelin-2 leads to reduced ovulation and CL formation.
PloS one9(4)e96115(2014)
Cacioppo JA,Oh SW,Kim HY,Cho J,Lin PC,Yanagisawa M,Ko C

Endothelin-2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis.
Proceedings of the National Academy of Sciences of the United States of America110(40)E3830-9(2013 Oct)
Rattner A,Yu H,Williams J,Smallwood PM,Nathans J

Identification of endothelin 2 as an inflammatory factor that promotes central nervous system remyelination.
Brain : a journal of neurology136(Pt 4)1035-47(2013 Apr)
Yuen TJ,Johnson KR,Miron VE,Zhao C,Quandt J,Harrisingh MC,Swire M,Williams A,McFarland HF,Franklin RJ,Ffrench-Constant C

Expression of endothelin-2 (ET-2) gene in a human renal adenocarcinoma cell line: purification and cDNA cloning of ET-2.
Journal of cardiovascular pharmacology17 Suppl 7S39-43(1991)
Onda H,Ohkubo S,Kosaka T,Yasuhara T,Ogi K,Hosoya M,Matsumoto H,Suzuki N,Kitada C,Ishibashi Y

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EDN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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