AGXT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGXT
Entrez Gene ID	189
Full Name	alanine-glyoxylate aminotransferase
Synonyms	AGT,AGT1,AGXT1,PH1,SPAT,SPT,TLH6
General protein information	Preferred Names alanine-glyoxylate aminotransferase Names serine--pyruvate aminotransferase L-alanine: glyoxylate aminotransferase 1 hepatic peroxisomal alanine:glyoxylate aminotransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.3
Summary	This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008].
Disorder MIM:	604285
Disorder Html:	Hyperoxaluria, primary, type 1, 259900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000030.3	NP_000021.1	serine--pyruvate aminotransferase
NM_000030.2	NP_000021.1	serine--pyruvate aminotransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC607355	XP_848328.2
Danio rerio (zebrafish)	agxta	NP_001002331.1
Neurospora crassa	NCU01821	XP_955918.1
Pan troglodytes (chimpanzee)	AGXT	XP_003309627.2
Bos taurus (cattle)	AGXT	NP_001095825.1
Rattus norvegicus (Norway rat)	Agxt	NP_085914.2
Drosophila melanogaster (fruit fly)	Spat	NP_511062.1
Saccharomyces cerevisiae (baker's yeast)	AGX1	NP_116623.1
Caenorhabditis elegans (roundworm)	T14D7.1	NP_495885.1
Magnaporthe oryzae (rice blast fungus)	MGG_02525	XP_003709335.1
Xenopus tropicalis (tropical clawed frog)	agxt	NP_001006705.1
Homo sapiens (human)	AGXT	NP_000021.1
Mus musculus (house mouse)	Agxt	NP_057911.2
Gallus gallus (chicken)	AGXT	XP_003641783.2
Kluyveromyces lactis	KLLA0F08954g	XP_455487.1
Arabidopsis thaliana (thale cress)	AGT	NP_178969.1
Oryza sativa (rice)	Os08g0502700	NP_001062170.1
	AGXT	XP_001090301.2
Canis lupus familiaris (dog)	LOC100855679	XP_003639939.1
Danio rerio (zebrafish)	agxtb	NP_998327.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003490	XP_309676.3

Gene Ontology
Bibliography

Related articles in PubMed

Mutational Analysis of Agxt in Tunisian Population with Primary Hyperoxaluria Type 1.
M'dimegh S, Omezzine A, M'barek I, Moussa A, Mabrouk S, Kaarout H, Souche G, Chemli J, Aloui S, Aquaviva-Bourdain C, Achour A, Abroug S, Bouslama A
Annals of human genetics81(1)1-10(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Novel AGXT mutations in a Tunisian population with primary hyperoxaluria type 1.
Title: Mutational Analysis of Agxt in Tunisian Population with Primary Hyperoxaluria Type 1.

Data show that onomeric minor allele of human alanine glyoxylate aminotransferase (AGT-Mi) binds pyridoxal 5-phosphate (PLP) but does not display catalytic activity.
Title: Effects of interface mutations on the dimerization of alanine glyoxylate aminotransferase and implications in the mistargeting of the pathogenic variants F152I and I244T.

AGXT gene sequencing is now the choice of diagnosis of Primary hyperoxaluria type 1 (PH1)due to its non-invasive nature compared to liver enzyme assay. Early diagnosis and accurate treatment in PH1 is important for better patient outcomes.
Title: Molecular analysis of the AGXT gene in patients suspected with hyperoxaluria type 1 and three novel mutations from Turkey.

this study identifies a novel AGXT gene mutation in primary hyperoxaluria after kidney transplantation failure in Tunisian patient
Title: Identification of a novel AGXT gene mutation in primary hyperoxaluria after kidney transplantation failure.

The novel p.Gln137Hisfs*19 AGXT mutation detected in this study extends the spectrum of known primary hyperoxaluria type I AGXT gene mutations in Tunisia.
Title: A novel mutation in the AGXT gene causing primary hyperoxaluria type I: genotype-phenotype correlation.

The following AGXT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGXT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18906	NM_000030.3 Latest version!	Homo sapiens alanine--glyoxylate and serine--pyruvate aminotransferase (AGXT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu18906	NM_000030.2	Homo sapiens alanine-glyoxylate aminotransferase (AGXT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGXT ( NM_000030.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000021.1 Homo sapiens alanine--glyoxylate and serine--pyruvate aminotransferase (AGXT), mRNA.

Price & Availability↑

CloneID	OHu18906
Clone ID Related Accession (Same CDS sequence)	NM_000030.3 , NM_000030.2
Accession Version	NM_000030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	serine--pyruvate aminotransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X53414.1, AA857309.1, CB156891.1 and AC104809.5. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2018 this sequence version replaced NM_000030.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292754.1, X53414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000307503.4/ ENSP00000302620.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC 
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC 
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC 
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC 
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA 
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG 
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC 
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC 
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC 
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC 
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC 
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC 
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG 
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG 
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA 
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA 
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG 
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG 
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000021.1
CDS	43..1221
Translation	MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

Target ORF information:

RefSeq Version	NM_000030.3
Organism	Homo sapiens(human)
Definition	Homo sapiens alanine--glyoxylate and serine--pyruvate aminotransferase (AGXT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000030.3

ORF Insert Sequence:

ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC 
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC 
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC 
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC 
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA 
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG 
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC 
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC 
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC 
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC 
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC 
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC 
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG 
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG 
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA 
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA 
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG 
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG 
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGXT ( NM_000030.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000021.1 Homo sapiens alanine-glyoxylate aminotransferase (AGXT), mRNA.

Price & Availability↑

CloneID	OHu18906
Clone ID Related Accession (Same CDS sequence)	NM_000030.3 , NM_000030.2
Accession Version	NM_000030.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	serine--pyruvate aminotransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X53414.1, AA857309.1 and CB156891.1. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced NM_000030.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53414.1, BC132819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC 
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC 
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC 
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC 
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA 
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG 
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC 
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC 
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC 
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC 
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC 
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC 
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG 
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG 
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA 
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA 
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG 
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG 
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000021.1
CDS	122..1300
Translation	MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

Target ORF information:

RefSeq Version	NM_000030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens alanine-glyoxylate aminotransferase (AGXT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000030.2

ORF Insert Sequence:

ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC 
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC 
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC 
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC 
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA 
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG 
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC 
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC 
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC 
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC 
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC 
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC 
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG 
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG 
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA 
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA 
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG 
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG 
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutational Analysis of Agxt in Tunisian Population with Primary Hyperoxaluria Type 1.
Annals of human genetics81(1)1-10(2017 Jan)
M'dimegh S,Omezzine A,M'barek I,Moussa A,Mabrouk S,Kaarout H,Souche G,Chemli J,Aloui S,Aquaviva-Bourdain C,Achour A,Abroug S,Bouslama A

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AGXT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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