E4F1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005255155.1	XP_005255212.1	transcription factor E4F1 isoform X1
XM_011522403.1	XP_011520705.1	transcription factor E4F1 isoform X5
XM_017023011.1	XP_016878500.1	transcription factor E4F1 isoform X6
NM_001288778.1	NP_001275707.1	transcription factor E4F1 isoform 3
XM_017023010.1	XP_016878499.1	transcription factor E4F1 isoform X4
XM_005255156.1	XP_005255213.1	transcription factor E4F1 isoform X2
NM_004424.4	NP_004415.3	transcription factor E4F1 isoform 1
NM_001288776.1	NP_001275705.1	transcription factor E4F1 isoform 2
XM_006720858.1	XP_006720921.1	transcription factor E4F1 isoform X7
NM_004424.5	NP_004415.4	transcription factor E4F1 isoform 1
XM_011522402.1	XP_011520704.1	transcription factor E4F1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27574	NM_004424.4 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu27640	NM_001288776.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu06414	NM_001288778.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu48775	XM_005255155.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu48776	XM_005255156.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu48778	XM_006720858.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu57414	XM_011522402.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu57415	XM_011522403.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu107368	NM_004424.5 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu85890	XM_017023010.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu85891	XM_017023011.1 Latest version!	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

E4F1 ( NM_004424.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004415.3 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27574
Clone ID Related Accession (Same CDS sequence)	NM_004424.4
Accession Version	NM_004424.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1 and BC080524.1. On Jan 4, 2014 this sequence version replaced NM_004424.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080524.1, U87269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004415.3
CDS	127..2481
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPHQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	NM_004424.4
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004424.4

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( NM_001288776.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275705.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27640
Clone ID Related Accession (Same CDS sequence)	NM_001288776.1
Accession Version	NM_001288776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1, BC014068.2 and BC080524.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region that results in a frameshift compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014068.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155628, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGCCACTG CGGACGATGC GGAGACCAGT GAGGCCACGG 
AGATCATCGA GGGCACCCAG ACAGAGGTGG ACAGCCACAT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275705.1
CDS	127..2091
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPHQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVPLRTMRRPVRPRRSSRAPRQRWTATS

Target ORF information:

RefSeq Version	NM_001288776.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288776.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGCCACTG CGGACGATGC GGAGACCAGT GAGGCCACGG 
AGATCATCGA GGGCACCCAG ACAGAGGTGG ACAGCCACAT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( NM_001288778.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275707.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06414
Clone ID Related Accession (Same CDS sequence)	NM_001288778.1
Accession Version	NM_001288778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1, AK225132.1, DN998026.1 and BC080524.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons and has two alternate in-frame splice sites in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225132.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGT TCTGCAGCCG TGGCTTCCGA 
GAGAAGGGCT CACTGGTGCG GCACGTGCGA CACCACACAG GCGAGAAGCC GTTCAAGTGC 
TACAAGTGCG GCCGTGGCTT CGCCGAGCAC GGCACGCTGA ACCGGCACCT GCGCACCAAA 
GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG CTGGTGTCTG AGGACAGCCC CGCGGCAGCC 
ACCACCGTCC TCACGGAAGA CCCGCACACA GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT 
GACACCCAGG AGTATATCAT CGAGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG 
GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG 
ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG 
GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG 
AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT 
GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG 
GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG 
GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275707.1
CDS	127..1950
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPHQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	NM_001288778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288778.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGT TCTGCAGCCG TGGCTTCCGA 
GAGAAGGGCT CACTGGTGCG GCACGTGCGA CACCACACAG GCGAGAAGCC GTTCAAGTGC 
TACAAGTGCG GCCGTGGCTT CGCCGAGCAC GGCACGCTGA ACCGGCACCT GCGCACCAAA 
GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG CTGGTGTCTG AGGACAGCCC CGCGGCAGCC 
ACCACCGTCC TCACGGAAGA CCCGCACACA GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT 
GACACCCAGG AGTATATCAT CGAGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG 
GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG 
ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG 
GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG 
AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT 
GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG 
GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG 
GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_005255155.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255212.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.

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CloneID	OHu48775
Clone ID Related Accession (Same CDS sequence)	XM_005255155.1
Accession Version	XM_005255155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG 
GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA 
GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG 
TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT 
GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT 
GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC 
TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC 
GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA 
CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC 
GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG 
CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA 
GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT GACACCCAGG AGTATATCAT CGAGGCCACT 
GCGGACGATG CGGAGACCAG TGAGGCCACG GAGATCATCG AGGGCACCCA GACAGAGGTG 
GACAGCCACA TCATGAAGGT GGTGCAGCAG ATCGTGCACC AGGCTAGCGC CGGCCACCAG 
ATCATCGTGC AGAACGTCAC CATGGACGAG GAGACGGCGC TGGGCCCAGA GGCGGCTGCC 
GCCGACACCA TCACCATCGC CACCCCCGAG AGCCTGACAG AGCAGGTGGC CATGACGCTG 
GCCTCGGCCA TCAGCGAGGG CACTGTGCTT GCCGCCCGGG CAGGGACAAG TGGCACTGAA 
CAGGCCACTG TGACCATGGT GTCATCAGAG GACATCGAGA TCCTGGAGCA TGCAGGCGAG 
CTGGTCATCG CCTCGCCGGA GGGCCAGCTG GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255212.1
CDS	117..2630
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEGNRADPEGAAGVRAVHPGLAEARMARAAGSTDPPRPRHLADLAGPSQPSTKPPDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_005255155.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255155.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG 
GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA 
GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG 
TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT 
GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT 
GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC 
TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC 
GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA 
CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC 
GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG 
CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA 
GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT GACACCCAGG AGTATATCAT CGAGGCCACT 
GCGGACGATG CGGAGACCAG TGAGGCCACG GAGATCATCG AGGGCACCCA GACAGAGGTG 
GACAGCCACA TCATGAAGGT GGTGCAGCAG ATCGTGCACC AGGCTAGCGC CGGCCACCAG 
ATCATCGTGC AGAACGTCAC CATGGACGAG GAGACGGCGC TGGGCCCAGA GGCGGCTGCC 
GCCGACACCA TCACCATCGC CACCCCCGAG AGCCTGACAG AGCAGGTGGC CATGACGCTG 
GCCTCGGCCA TCAGCGAGGG CACTGTGCTT GCCGCCCGGG CAGGGACAAG TGGCACTGAA 
CAGGCCACTG TGACCATGGT GTCATCAGAG GACATCGAGA TCCTGGAGCA TGCAGGCGAG 
CTGGTCATCG CCTCGCCGGA GGGCCAGCTG GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_005255156.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255213.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu48776
Clone ID Related Accession (Same CDS sequence)	XM_005255156.1
Accession Version	XM_005255156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC 
TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC 
ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT 
GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG 
CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC 
CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC 
AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG 
TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC 
CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC 
ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG 
GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG 
TTGGTGGAGT TCTCGTCCGT GGTAGCTGAC ACCCAGGAGT ATATCATCGA GGCCACTGCG 
GACGATGCGG AGACCAGTGA GGCCACGGAG ATCATCGAGG GCACCCAGAC AGAGGTGGAC 
AGCCACATCA TGAAGGTGGT GCAGCAGATC GTGCACCAGG CTAGCGCCGG CCACCAGATC 
ATCGTGCAGA ACGTCACCAT GGACGAGGAG ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC 
GACACCATCA CCATCGCCAC CCCCGAGAGC CTGACAGAGC AGGTGGCCAT GACGCTGGCC 
TCGGCCATCA GCGAGGGCAC TGTGCTTGCC GCCCGGGCAG GGACAAGTGG CACTGAACAG 
GCCACTGTGA CCATGGTGTC ATCAGAGGAC ATCGAGATCC TGGAGCATGC AGGCGAGCTG 
GTCATCGCCT CGCCGGAGGG CCAGCTGGAG GTGCAGACGG TCATCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255213.1
CDS	117..2627
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEGNRADPEGAAGVRAVHPGLAEARMARAAGSTDPPRPRHLADLAGPSQPSTKPPDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_005255156.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255156.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC 
TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC 
ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT 
GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG 
CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC 
CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC 
AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG 
TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC 
CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC 
ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG 
GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG 
TTGGTGGAGT TCTCGTCCGT GGTAGCTGAC ACCCAGGAGT ATATCATCGA GGCCACTGCG 
GACGATGCGG AGACCAGTGA GGCCACGGAG ATCATCGAGG GCACCCAGAC AGAGGTGGAC 
AGCCACATCA TGAAGGTGGT GCAGCAGATC GTGCACCAGG CTAGCGCCGG CCACCAGATC 
ATCGTGCAGA ACGTCACCAT GGACGAGGAG ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC 
GACACCATCA CCATCGCCAC CCCCGAGAGC CTGACAGAGC AGGTGGCCAT GACGCTGGCC 
TCGGCCATCA GCGAGGGCAC TGTGCTTGCC GCCCGGGCAG GGACAAGTGG CACTGAACAG 
GCCACTGTGA CCATGGTGTC ATCAGAGGAC ATCGAGATCC TGGAGCATGC AGGCGAGCTG 
GTCATCGCCT CGCCGGAGGG CCAGCTGGAG GTGCAGACGG TCATCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_006720858.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720921.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu48778
Clone ID Related Accession (Same CDS sequence)	XM_006720858.1
Accession Version	XM_006720858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT 
GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG 
CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG 
ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT 
CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC 
CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA 
CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC 
CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG 
CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC 
ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG 
TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG 
GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG 
GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG 
GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG 
CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA 
GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC 
CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG 
GTCATCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720921.1
CDS	49..2400
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_006720858.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720858.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT 
GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG 
CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG 
ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT 
CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC 
CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA 
CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC 
CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG 
CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC 
ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG 
TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG 
GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG 
GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG 
GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG 
CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA 
GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC 
CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG 
GTCATCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_011522402.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520704.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu57414
Clone ID Related Accession (Same CDS sequence)	XM_011522402.1
Accession Version	XM_011522402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG 
GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA 
GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG 
TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT 
GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT 
GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC 
TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC 
GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA 
CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC 
GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG 
CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA 
GTGTTGGTGG AGTTCTCGTC CGTGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG 
GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG 
ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG 
GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG 
AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT 
GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG 
GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG 
GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520704.1
CDS	117..2600
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEGNRADPEGAAGVRAVHPGLAEARMARAAGSTDPPRPRHLADLAGPSQPSTKPPDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_011522402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522402.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG 
GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA 
GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG 
TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT 
GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT 
GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC 
TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC 
GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA 
CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC 
GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG 
CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA 
GTGTTGGTGG AGTTCTCGTC CGTGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG 
GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG 
ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG 
GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG 
AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT 
GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG 
GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG 
GAGGTGCAGA CGGTCATCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_011522403.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520705.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.

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CloneID	OHu57415
Clone ID Related Accession (Same CDS sequence)	XM_011522403.1
Accession Version	XM_011522403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA 
GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC 
GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC 
GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520705.1
CDS	77..2551
Translation	MVVNPPSSCGALRHGGRDGSAGDGRSYGRSPGRSRAGSGRGCSCGGGGGLGPQRLPRPPGALQRGRAGGKARRSPGCPASAFPGPADSRLVADEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_011522403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522403.1

ORF Insert Sequence:

ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA 
GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC 
GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC 
GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( NM_004424.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004415.4 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

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CloneID	OHu107368
Clone ID Related Accession (Same CDS sequence)	NM_004424.5
Accession Version	NM_004424.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009065.8. On Nov 23, 2018 this sequence version replaced NM_004424.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080524.1, SRR3476690.890332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301727.9/ ENSP00000301727.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004415.4
CDS	24..2378
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	NM_004424.5
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004424.5

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC 
AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG 
AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG 
CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC 
AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT 
TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA 
CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC 
TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC 
GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC 
CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC 
CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG 
GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC 
GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC 
CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG 
CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA 
GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG 
GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG 
ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG 
ACGGTCATCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_017023010.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878499.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu85890
Clone ID Related Accession (Same CDS sequence)	XM_017023010.1
Accession Version	XM_017023010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC 
TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC 
ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT 
GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG 
CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC 
CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC 
AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG 
TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC 
CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC 
ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG 
GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG 
TTGGTGGAGT TCTCGTCCGT GGCCACTGCG GACGATGCGG AGACCAGTGA GGCCACGGAG 
ATCATCGAGG GCACCCAGAC AGAGGTGGAC AGCCACATCA TGAAGGTGGT GCAGCAGATC 
GTGCACCAGG CTAGCGCCGG CCACCAGATC ATCGTGCAGA ACGTCACCAT GGACGAGGAG 
ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC GACACCATCA CCATCGCCAC CCCCGAGAGC 
CTGACAGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTGCC 
GCCCGGGCAG GGACAAGTGG CACTGAACAG GCCACTGTGA CCATGGTGTC ATCAGAGGAC 
ATCGAGATCC TGGAGCATGC AGGCGAGCTG GTCATCGCCT CGCCGGAGGG CCAGCTGGAG 
GTGCAGACGG TCATCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878499.1
CDS	117..2597
Translation	MEGAMAVRVTAAHTAEAQAEAGREAGEGAVAAVAAALAPSGFLGLPAPFSEEGNRADPEGAAGVRAVHPGLAEARMARAAGSTDPPRPRHLADLAGPSQPSTKPPDEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_017023010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023010.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC 
GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT 
GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG 
GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC 
TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC 
ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG 
GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA 
GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG 
GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC 
GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC 
CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC 
AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC 
AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC 
TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC 
TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC 
AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC 
AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA 
TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC 
AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA 
GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT 
GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG 
GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG 
GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC 
TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC 
ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT 
GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG 
CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC 
CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC 
AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG 
TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC 
CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC 
ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG 
GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG 
TTGGTGGAGT TCTCGTCCGT GGCCACTGCG GACGATGCGG AGACCAGTGA GGCCACGGAG 
ATCATCGAGG GCACCCAGAC AGAGGTGGAC AGCCACATCA TGAAGGTGGT GCAGCAGATC 
GTGCACCAGG CTAGCGCCGG CCACCAGATC ATCGTGCAGA ACGTCACCAT GGACGAGGAG 
ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC GACACCATCA CCATCGCCAC CCCCGAGAGC 
CTGACAGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTGCC 
GCCCGGGCAG GGACAAGTGG CACTGAACAG GCCACTGTGA CCATGGTGTC ATCAGAGGAC 
ATCGAGATCC TGGAGCATGC AGGCGAGCTG GTCATCGCCT CGCCGGAGGG CCAGCTGGAG 
GTGCAGACGG TCATCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4F1 ( XM_017023011.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878500.1 Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu85891
Clone ID Related Accession (Same CDS sequence)	XM_017023011.1
Accession Version	XM_017023011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor E4F1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA 
GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC 
GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC 
GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT 
GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG 
CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG 
ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT 
CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC 
CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA 
CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC 
CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG 
CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC 
ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG 
TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG 
GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG 
GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG 
GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG 
CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA 
GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC 
CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG 
GTCATCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878500.1
CDS	77..2548
Translation	MVVNPPSSCGALRHGGRDGSAGDGRSYGRSPGRSRAGSGRGCSCGGGGGLGPQRLPRPPGALQRGRAGGKARRSPGCPASAFPGPADSRLVADEDDVHRCGRCQAEFTALEDFVQHKIQKACQRAPPEALPATPATTALLGQEVVPAAPGPEEPITVAHIVVEAASLAADISHASDLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGEGEQAQVKLLVNKDGRYVCALCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCSKCGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDARAGSGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGTVIHEVHVQMQELSLGMKALAPEPPVSQELPCSSEGSRENLLHQAMQNSGIVLERAAGEEGALEPAPAAGSSPQPLAVAAPQLPVLEVQPLETVASEASAVPRTHPCPQCSETFPTAATLEAHKRGHTGPRPFACAQCGKAFPKAYLLKKHQEVHVRERRFRCGDCGKLYKTIAHVRGHRRVHSDERPYPCPKCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEDSPAAATTVLTEDPHTVLVEFSSVVADTQEYIIEATADDAETSEATEIIEGTQTEVDSHIMKVVQQIVHQASAGHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_017023011.1
Organism	Homo sapiens(human)
Definition	Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023011.1

ORF Insert Sequence:

ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA 
GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC 
GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC 
GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC 
GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG 
GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG 
GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC 
GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT 
GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC 
GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC 
CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC 
TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC 
GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG 
CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG 
GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT 
GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT 
GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG 
ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG 
GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC 
TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC 
GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC 
AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG 
GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT 
GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG 
CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG 
ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT 
CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC 
CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA 
CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC 
CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG 
CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC 
ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG 
TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG 
GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG 
GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG 
GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG 
CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA 
GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC 
CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG 
GTCATCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	E4F1
Entrez Gene ID	1877
Full Name	E4F transcription factor 1
Synonyms	E4F
General protein information	Preferred Names E4F transcription factor 1 Names transcription factor E4F1 RING-type E3 ubiquitin transferase E4F1 p120E4F p50E4F putative E3 ubiquitin-protein ligase E4F1 transcription factor E4F
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. [provided by RefSeq, Jan 2014].
Disorder MIM:	603022

Homo sapiens (human)	E4F1	NP_004415.3
Canis lupus familiaris (dog)	E4F1	XP_005622145.1
Gallus gallus (chicken)	E4F1	XP_414860.4
Xenopus tropicalis (tropical clawed frog)	e4f1	NP_001072166.1
Pan troglodytes (chimpanzee)	E4F1	XP_511205.3
Rattus norvegicus (Norway rat)	E4f1	NP_001171975.1
	E4F1	XP_001084651.1
Bos taurus (cattle)	E4F1	NP_001192754.1
Mus musculus (house mouse)	E4f1	NP_031919.2
Danio rerio (zebrafish)	e4f1	NP_001020684.1

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E4F1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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