DPH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPH1
Entrez Gene ID	1801
Full Name	diphthamide biosynthesis 1
Synonyms	DEDSSH,DPH2L,DPH2L1,OVCA1
General protein information	Preferred Names diphthamide biosynthesis 1 Names 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 DPH-like 1 DPH1 homolog DPH2-like 1 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1 candidate tumor suppressor in ovarian cancer 1 diphthamide biosynthesis protein 1 diphthamide biosynthesis protein 2 homolog-like 1 diphtheria toxin resistance protein 1 diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1 ovarian cancer-associated gene 1 protein ovarian tumor suppressor candidate 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.3
Summary	The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016].
Disorder MIM:	603527
Disorder Html:	Developmental delay with short stature, dysmorphic features, and sparse hair, 616901 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346576.1	NP_001333505.1	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 4
NM_001383.5	NP_001374.4	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 1
NM_001383.4	NP_001374.3	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 1
NM_001346574.1	NP_001333503.1	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 2
NM_001346575.1	NP_001333504.1	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DPH1	NP_001070367.1
Mus musculus (house mouse)	Dph1	NP_652762.2
Rattus norvegicus (Norway rat)	Dph1	NP_001099279.1
Danio rerio (zebrafish)	dph1	NP_001017861.1
Drosophila melanogaster (fruit fly)	CG11652	NP_648496.1
Xenopus tropicalis (tropical clawed frog)	dph1	NP_001135627.1
Homo sapiens (human)	DPH1	NP_001374.3
	LOC721275	XP_002808156.1
Gallus gallus (chicken)	DPH1	NP_001025887.1
Kluyveromyces lactis	KLLA0F03971g	XP_455260.1
Schizosaccharomyces pombe (fission yeast)	SPBC3B8.05	NP_596409.1
Saccharomyces cerevisiae (baker's yeast)	DPH1	NP_012163.1
Arabidopsis thaliana (thale cress)	AT5G62030	NP_201009.1
Pan troglodytes (chimpanzee)	DPH1	XP_511255.2
Neurospora crassa	NCU08503	XP_963432.1
Oryza sativa (rice)	Os02g0815600	NP_001048504.1
Caenorhabditis elegans (roundworm)	dph-1	NP_497750.1
Eremothecium gossypii	AGOS_ADL229W	NP_983867.1
Magnaporthe oryzae (rice blast fungus)	MGG_03237	XP_003716739.1

Gene Ontology
Bibliography

Related articles in PubMed

Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p.
Liu M, Yin K, Guo X, Feng H, Yuan M, Liu Y, Zhang J, Guo B, Wang C, Zhou G, Zhou Z, Zhang CY, Chen X
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(2)505-514(2017)

Matching two independent cohorts validates DPH1 as a gene responsible for autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
Loucks CM, Parboosingh JS, Shaheen R, Bernier FP, McLeod DR, Seidahmed MZ, Puffenberger EG, Ober C, Hegele RA, Boycott KM, Alkuraya FS, Innes AM
Human mutation36(10)1015-9(2015 Oct)

Loss of diphthamide pre-activates NF-?B and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor.
Stahl S, da Silva Mateus Seidl AR, Ducret A, Kux van Geijtenbeek S, Michel S, Racek T, Birzele F, Haas AK, Rueger R, Gerg M, Niederfellner G, Pastan I, Brinkmann U
Proceedings of the National Academy of Sciences of the United States of America112(34)10732-7(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DPH1 functions as an oncogene in CRC and can be negatively modulated by miR-218-5p to promote CRC tumourigenesis.
Title: Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p.

Results identified a second homozygous missense variant in DPH1, seen in four members of a founder population, and associated with autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
Title: Matching two independent cohorts validates DPH1 as a gene responsible for autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.

Methylation of the DPH1 promoter causes immunotoxin resistance in acute lymphoblastic leukemia.
Title: Methylation of the DPH1 promoter causes immunotoxin resistance in acute lymphoblastic leukemia cell line KOPN-8.

OVCA1 could inhibit the proliferation of ovarian cancer cells by p16/cyclin D1 pathway, but not by NF-kappaB
Title: OVCA1 inhibits the proliferation of epithelial ovarian cancer cells by decreasing cyclin D1 and increasing p16.

The following DPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu109470	NM_001383.5 Latest version!	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu101119	NM_001346574.1 Latest version!	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu101120	NM_001346576.1 Latest version!	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu101124	NM_001346575.1 Latest version!	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu30111	NM_001383.4 Latest version!	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPH1 ( NM_001383.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001374.4 Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu109470
Clone ID Related Accession (Same CDS sequence)	NM_001383.5
Accession Version	NM_001383.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U34880.1, AF321876.1 and AC090617.16. On Sep 24, 2019 this sequence version replaced NM_001383.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003099.1, AF321876.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC TGGTCGTATC CGGGGCAGCG GAGCAGGGCG GCCGAGACGG CCCTGGCAGA 
GGTCGGGCCC CTCGGGGCCG CGTGGCCAAT CAGATCCCCC CTGAGATCCT GAAGAACCCT 
CAGCTGCAGG CAGCAATCCG GGTCCTGCCT TCCAACTACA ACTTTGAGAT CCCCAAGACC 
ATCTGGAGGA TCCAACAAGC CCAGGCCAAG AAGGTGGCCT TGCAAATGCC GGAAGGCCTC 
CTCCTCTTTG CCTGTACCAT TGTGGATATC TTGGAAAGGT TCACGGAGGC CGAAGTGATG 
GTGATGGGTG ACGTGACCTA CGGGGCTTGC TGTGTGGATG ACTTCACAGC GAGGGCCCTG 
GGAGCTGACT TCTTGGTGCA CTACGGCCAC AGTTGCCTGA TTCCCATGGA CACCTCGGCC 
CAAGACTTCC GGGTGCTGTA CGTCTTTGTG GACATCCGGA TAGACACTAC ACACCTCCTG 
GACTCTCTCC GCCTCACCTT TCCCCCAGCC ACTGCCCTTG CCCTGGTCAG CACCATTCAG 
TTTGTGTCGA CCTTGCAGGC AGCCGCCCAG GAGCTGAAAG CCGAGTATCG TGTGAGTGTC 
CCACAGTGCA AGCCCCTGTC CCCTGGAGAG ATCCTGGGCT GCACATCCCC CCGACTGTCC 
AAAGAGGTGG AGGCCGTTGT GTATCTTGGA GATGGCCGCT TCCATCTGGA GTCTGTCATG 
ATTGCCAACC CCAATGTCCC CGCTTACCGG TATGACCCAT ATAGCAAAGT CCTATCCAGA 
GAACACTATG ACCACCAGCG CATGCAGGCT GCTCGCCAAG AAGCCATAGC CACTGCCCGC 
TCAGCTAAGT CCTGGGGCCT TATTCTGGGC ACTTTGGGCC GCCAGGGCAG TCCTAAGATC 
CTGGAGCACC TGGAATCTCG ACTCCGAGCC TTGGGCCTTT CCTTTGTGAG GCTGCTGCTC 
TCTGAGATCT TCCCCAGCAA GCTTAGCCTA CTTCCTGAGG TGGATGTGTG GGTGCAGGTG 
GCATGTCCAC GTCTCTCCAT TGACTGGGGC ACAGCCTTCC CCAAGCCGCT GCTGACACCC 
TATGAGGCGG CCGTGGCTCT GAGGGACATT TCCTGGCAGC AGCCCTACCC GATGGACTTC 
TACGCTGGCA GCTCCTTGGG GCCCTGGACG GTGAACCACG GCCAGGACCG CCGTCCCCAC 
GCCCCGGGCC GGCCCGCGCG GGGGAAGGTG CAGGAGGGGT CCGCGCGTCC CCCTTCGGCC 
GTGGCTTGCG AGGACTGCAG CTGCAGGGAC GAGAAGGTGG CGCCGCTGGC TCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001374.4
CDS	18..1334
Translation	MAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Target ORF information:

RefSeq Version	NM_001383.5
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001383.5

ORF Insert Sequence:

ATGGCGGCGC TGGTCGTATC CGGGGCAGCG GAGCAGGGCG GCCGAGACGG CCCTGGCAGA 
GGTCGGGCCC CTCGGGGCCG CGTGGCCAAT CAGATCCCCC CTGAGATCCT GAAGAACCCT 
CAGCTGCAGG CAGCAATCCG GGTCCTGCCT TCCAACTACA ACTTTGAGAT CCCCAAGACC 
ATCTGGAGGA TCCAACAAGC CCAGGCCAAG AAGGTGGCCT TGCAAATGCC GGAAGGCCTC 
CTCCTCTTTG CCTGTACCAT TGTGGATATC TTGGAAAGGT TCACGGAGGC CGAAGTGATG 
GTGATGGGTG ACGTGACCTA CGGGGCTTGC TGTGTGGATG ACTTCACAGC GAGGGCCCTG 
GGAGCTGACT TCTTGGTGCA CTACGGCCAC AGTTGCCTGA TTCCCATGGA CACCTCGGCC 
CAAGACTTCC GGGTGCTGTA CGTCTTTGTG GACATCCGGA TAGACACTAC ACACCTCCTG 
GACTCTCTCC GCCTCACCTT TCCCCCAGCC ACTGCCCTTG CCCTGGTCAG CACCATTCAG 
TTTGTGTCGA CCTTGCAGGC AGCCGCCCAG GAGCTGAAAG CCGAGTATCG TGTGAGTGTC 
CCACAGTGCA AGCCCCTGTC CCCTGGAGAG ATCCTGGGCT GCACATCCCC CCGACTGTCC 
AAAGAGGTGG AGGCCGTTGT GTATCTTGGA GATGGCCGCT TCCATCTGGA GTCTGTCATG 
ATTGCCAACC CCAATGTCCC CGCTTACCGG TATGACCCAT ATAGCAAAGT CCTATCCAGA 
GAACACTATG ACCACCAGCG CATGCAGGCT GCTCGCCAAG AAGCCATAGC CACTGCCCGC 
TCAGCTAAGT CCTGGGGCCT TATTCTGGGC ACTTTGGGCC GCCAGGGCAG TCCTAAGATC 
CTGGAGCACC TGGAATCTCG ACTCCGAGCC TTGGGCCTTT CCTTTGTGAG GCTGCTGCTC 
TCTGAGATCT TCCCCAGCAA GCTTAGCCTA CTTCCTGAGG TGGATGTGTG GGTGCAGGTG 
GCATGTCCAC GTCTCTCCAT TGACTGGGGC ACAGCCTTCC CCAAGCCGCT GCTGACACCC 
TATGAGGCGG CCGTGGCTCT GAGGGACATT TCCTGGCAGC AGCCCTACCC GATGGACTTC 
TACGCTGGCA GCTCCTTGGG GCCCTGGACG GTGAACCACG GCCAGGACCG CCGTCCCCAC 
GCCCCGGGCC GGCCCGCGCG GGGGAAGGTG CAGGAGGGGT CCGCGCGTCC CCCTTCGGCC 
GTGGCTTGCG AGGACTGCAG CTGCAGGGAC GAGAAGGTGG CGCCGCTGGC TCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH1 ( NM_001346574.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333503.1 Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101119
Clone ID Related Accession (Same CDS sequence)	NM_001346574.1
Accession Version	NM_001346574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099684.7 and AC090617.16. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.9214.1, SRR1163657.222151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCACA CCTGGAATCT CGACTCCGAG CCTTGGGCCT TTCCTTTGTG 
AGGCTGCTGC TCTCTGAGAT CTTCCCCAGC AAGCTTAGCC TACTTCCTGA GGTGGATGTG 
TGGGTGCAGG TGGCATGTCC ACGTCTCTCC ATTGACTGGG GCACAGCCTT CCCCAAGCCG 
CTGCTGACAC CCTATGAGGC GGCCGTGGCT CTGAGGGACA TTTCCTGGCA GCAGCCCTAC 
CCGATGGACT TCTACGCTGG CAGCTCCTTG GGGCCCTGGA CGGTGAACCA CGGCCAGGAC 
CGCCGTCCCC ACGCCCCGGG CCGGCCCGCG CGGGGGAAGG TGCAGGAGGG GTCCGCGCGT 
CCCCCTTCGG CCGTGGCTTG CGAGGACTGC AGCTGCAGGG ACGAGAAGGT GGCGCCGCTG 
GCTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333503.1
CDS	44..1312
Translation	MRRQVMAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Target ORF information:

RefSeq Version	NM_001346574.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346574.1

ORF Insert Sequence:

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCACA CCTGGAATCT CGACTCCGAG CCTTGGGCCT TTCCTTTGTG 
AGGCTGCTGC TCTCTGAGAT CTTCCCCAGC AAGCTTAGCC TACTTCCTGA GGTGGATGTG 
TGGGTGCAGG TGGCATGTCC ACGTCTCTCC ATTGACTGGG GCACAGCCTT CCCCAAGCCG 
CTGCTGACAC CCTATGAGGC GGCCGTGGCT CTGAGGGACA TTTCCTGGCA GCAGCCCTAC 
CCGATGGACT TCTACGCTGG CAGCTCCTTG GGGCCCTGGA CGGTGAACCA CGGCCAGGAC 
CGCCGTCCCC ACGCCCCGGG CCGGCCCGCG CGGGGGAAGG TGCAGGAGGG GTCCGCGCGT 
CCCCCTTCGG CCGTGGCTTG CGAGGACTGC AGCTGCAGGG ACGAGAAGGT GGCGCCGCTG 
GCTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH1 ( NM_001346576.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333505.1 Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101120
Clone ID Related Accession (Same CDS sequence)	NM_001346576.1
Accession Version	NM_001346576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U34880.1, BC096088.1, AC090617.16 and AF321876.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCAGC TAAGTCCTGG GGCCTTATTC TGGGCACTTT GGGCCGCCAG 
GGCAGTCCTA AGATCCTGGA GCACCTGGAA TCTCGACTCC GAGCCTTGGG CCTTTCCTTT 
GTGAGGCTGC TGCTCTCTGA GATCTTCCCC AGCAAGCTTA GCCTACTTCC TGAGGTGGAT 
GTGTGGGTGC AGGTGGCATG TCCACGTCTC TCCATTGACT GGGGCACAGC CTTCCCCAAG 
CCGCTGCTGA CACCCTATGA GGCGGCCGTG GCTCTGAGGG ACATTTCCTG GCAGCAGCCC 
TACCCGATGG ACTTCTACGC TGGCAGCTCC TTGGGGCCCT GGACGGTGAA CCACGGCCAG 
GACCGCCGTC CCCACGCCCC GGGCCGGCCC GCGCGGGGGA AGGTGCAGGA GGGGTCCGCG 
CGTCCCCCTT CGGCCGTGGC TTGCGAGGAC TGCAGCTGCA GGGACGAGAA GGTGGCGCCG 
CTGGCTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333505.1
CDS	342..1253
Translation	MDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Target ORF information:

RefSeq Version	NM_001346576.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346576.1

ORF Insert Sequence:

ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCAGC TAAGTCCTGG GGCCTTATTC TGGGCACTTT GGGCCGCCAG 
GGCAGTCCTA AGATCCTGGA GCACCTGGAA TCTCGACTCC GAGCCTTGGG CCTTTCCTTT 
GTGAGGCTGC TGCTCTCTGA GATCTTCCCC AGCAAGCTTA GCCTACTTCC TGAGGTGGAT 
GTGTGGGTGC AGGTGGCATG TCCACGTCTC TCCATTGACT GGGGCACAGC CTTCCCCAAG 
CCGCTGCTGA CACCCTATGA GGCGGCCGTG GCTCTGAGGG ACATTTCCTG GCAGCAGCCC 
TACCCGATGG ACTTCTACGC TGGCAGCTCC TTGGGGCCCT GGACGGTGAA CCACGGCCAG 
GACCGCCGTC CCCACGCCCC GGGCCGGCCC GCGCGGGGGA AGGTGCAGGA GGGGTCCGCG 
CGTCCCCCTT CGGCCGTGGC TTGCGAGGAC TGCAGCTGCA GGGACGAGAA GGTGGCGCCG 
CTGGCTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH1 ( NM_001346575.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333504.1 Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 3, mRNA.

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CloneID	OHu101124
Clone ID Related Accession (Same CDS sequence)	NM_001346575.1
Accession Version	NM_001346575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099684.7 and AC090617.16. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.94333.1, SRR1660803.116714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GCAGCCGCCC AGGAGCTGAA AGCCGAGTAT CGTGTGAGTG TCCCACAGTG CAAGCCCCTG 
TCCCCTGGAG AGATCCTGGG CTGCACATCC CCCCGACTGT CCAAAGAGGT GGAGGCCGTT 
GTGTATCTTG GAGATGGCCG CTTCCATCTG GAGTCTGTCA TGATTGCCAA CCCCAATGTC 
CCCGCTTACC GGTATGACCC ATATAGCAAA GTCCTATCCA GAGAACACTA TGACCACCAG 
CGCATGCAGG CTGCTCGCCA AGAAGCCATA GCCACTGCCC GCTCACACCT GGAATCTCGA 
CTCCGAGCCT TGGGCCTTTC CTTTGTGAGG CTGCTGCTCT CTGAGATCTT CCCCAGCAAG 
CTTAGCCTAC TTCCTGAGGT GGATGTGTGG GTGCAGGTGG CATGTCCACG TCTCTCCATT 
GACTGGGGCA CAGCCTTCCC CAAGCCGCTG CTGACACCCT ATGAGGCGGC CGTGGCTCTG 
AGGGACATTT CCTGGCAGCA GCCCTACCCG ATGGACTTCT ACGCTGGCAG CTCCTTGGGG 
CCCTGGACGG TGAACCACGG CCAGGACCGC CGTCCCCACG CCCCGGGCCG GCCCGCGCGG 
GGGAAGGTGC AGGAGGGGTC CGCGCGTCCC CCTTCGGCCG TGGCTTGCGA GGACTGCAGC 
TGCAGGGACG AGAAGGTGGC GCCGCTGGCT CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333504.1
CDS	44..1279
Translation	MRRQVMAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Target ORF information:

RefSeq Version	NM_001346575.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346575.1

ORF Insert Sequence:

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GCAGCCGCCC AGGAGCTGAA AGCCGAGTAT CGTGTGAGTG TCCCACAGTG CAAGCCCCTG 
TCCCCTGGAG AGATCCTGGG CTGCACATCC CCCCGACTGT CCAAAGAGGT GGAGGCCGTT 
GTGTATCTTG GAGATGGCCG CTTCCATCTG GAGTCTGTCA TGATTGCCAA CCCCAATGTC 
CCCGCTTACC GGTATGACCC ATATAGCAAA GTCCTATCCA GAGAACACTA TGACCACCAG 
CGCATGCAGG CTGCTCGCCA AGAAGCCATA GCCACTGCCC GCTCACACCT GGAATCTCGA 
CTCCGAGCCT TGGGCCTTTC CTTTGTGAGG CTGCTGCTCT CTGAGATCTT CCCCAGCAAG 
CTTAGCCTAC TTCCTGAGGT GGATGTGTGG GTGCAGGTGG CATGTCCACG TCTCTCCATT 
GACTGGGGCA CAGCCTTCCC CAAGCCGCTG CTGACACCCT ATGAGGCGGC CGTGGCTCTG 
AGGGACATTT CCTGGCAGCA GCCCTACCCG ATGGACTTCT ACGCTGGCAG CTCCTTGGGG 
CCCTGGACGG TGAACCACGG CCAGGACCGC CGTCCCCACG CCCCGGGCCG GCCCGCGCGG 
GGGAAGGTGC AGGAGGGGTC CGCGCGTCCC CCTTCGGCCG TGGCTTGCGA GGACTGCAGC 
TGCAGGGACG AGAAGGTGGC GCCGCTGGCT CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH1 ( NM_001383.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001374.3 Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.

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CloneID	OHu30111
Clone ID Related Accession (Same CDS sequence)	NM_001383.4
Accession Version	NM_001383.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U34880.1, AF321876.1 and AC090617.16. This sequence is a reference standard in the RefSeqGene project. On Oct 20, 2016 this sequence version replaced NM_001383.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF321876.1, SRR1803615.126737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCAGC TAAGTCCTGG GGCCTTATTC TGGGCACTTT GGGCCGCCAG 
GGCAGTCCTA AGATCCTGGA GCACCTGGAA TCTCGACTCC GAGCCTTGGG CCTTTCCTTT 
GTGAGGCTGC TGCTCTCTGA GATCTTCCCC AGCAAGCTTA GCCTACTTCC TGAGGTGGAT 
GTGTGGGTGC AGGTGGCATG TCCACGTCTC TCCATTGACT GGGGCACAGC CTTCCCCAAG 
CCGCTGCTGA CACCCTATGA GGCGGCCGTG GCTCTGAGGG ACATTTCCTG GCAGCAGCCC 
TACCCGATGG ACTTCTACGC TGGCAGCTCC TTGGGGCCCT GGACGGTGAA CCACGGCCAG 
GACCGCCGTC CCCACGCCCC GGGCCGGCCC GCGCGGGGGA AGGTGCAGGA GGGGTCCGCG 
CGTCCCCCTT CGGCCGTGGC TTGCGAGGAC TGCAGCTGCA GGGACGAGAA GGTGGCGCCG 
CTGGCTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001374.3
CDS	44..1375
Translation	MRRQVMAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Target ORF information:

RefSeq Version	NM_001383.4
Organism	Homo sapiens(human)
Definition	Homo sapiens diphthamide biosynthesis 1 (DPH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001383.4

ORF Insert Sequence:

ATGCGCAGGC AGGTGATGGC GGCGCTGGTC GTATCCGGGG CAGCGGAGCA GGGCGGCCGA 
GACGGCCCTG GCAGAGGTCG GGCCCCTCGG GGCCGCGTGG CCAATCAGAT CCCCCCTGAG 
ATCCTGAAGA ACCCTCAGCT GCAGGCAGCA ATCCGGGTCC TGCCTTCCAA CTACAACTTT 
GAGATCCCCA AGACCATCTG GAGGATCCAA CAAGCCCAGG CCAAGAAGGT GGCCTTGCAA 
ATGCCGGAAG GCCTCCTCCT CTTTGCCTGT ACCATTGTGG ATATCTTGGA AAGGTTCACG 
GAGGCCGAAG TGATGGTGAT GGGTGACGTG ACCTACGGGG CTTGCTGTGT GGATGACTTC 
ACAGCGAGGG CCCTGGGAGC TGACTTCTTG GTGCACTACG GCCACAGTTG CCTGATTCCC 
ATGGACACCT CGGCCCAAGA CTTCCGGGTG CTGTACGTCT TTGTGGACAT CCGGATAGAC 
ACTACACACC TCCTGGACTC TCTCCGCCTC ACCTTTCCCC CAGCCACTGC CCTTGCCCTG 
GTCAGCACCA TTCAGTTTGT GTCGACCTTG CAGGCAGCCG CCCAGGAGCT GAAAGCCGAG 
TATCGTGTGA GTGTCCCACA GTGCAAGCCC CTGTCCCCTG GAGAGATCCT GGGCTGCACA 
TCCCCCCGAC TGTCCAAAGA GGTGGAGGCC GTTGTGTATC TTGGAGATGG CCGCTTCCAT 
CTGGAGTCTG TCATGATTGC CAACCCCAAT GTCCCCGCTT ACCGGTATGA CCCATATAGC 
AAAGTCCTAT CCAGAGAACA CTATGACCAC CAGCGCATGC AGGCTGCTCG CCAAGAAGCC 
ATAGCCACTG CCCGCTCAGC TAAGTCCTGG GGCCTTATTC TGGGCACTTT GGGCCGCCAG 
GGCAGTCCTA AGATCCTGGA GCACCTGGAA TCTCGACTCC GAGCCTTGGG CCTTTCCTTT 
GTGAGGCTGC TGCTCTCTGA GATCTTCCCC AGCAAGCTTA GCCTACTTCC TGAGGTGGAT 
GTGTGGGTGC AGGTGGCATG TCCACGTCTC TCCATTGACT GGGGCACAGC CTTCCCCAAG 
CCGCTGCTGA CACCCTATGA GGCGGCCGTG GCTCTGAGGG ACATTTCCTG GCAGCAGCCC 
TACCCGATGG ACTTCTACGC TGGCAGCTCC TTGGGGCCCT GGACGGTGAA CCACGGCCAG 
GACCGCCGTC CCCACGCCCC GGGCCGGCCC GCGCGGGGGA AGGTGCAGGA GGGGTCCGCG 
CGTCCCCCTT CGGCCGTGGC TTGCGAGGAC TGCAGCTGCA GGGACGAGAA GGTGGCGCCG 
CTGGCTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(2)505-514(2017)
Liu M,Yin K,Guo X,Feng H,Yuan M,Liu Y,Zhang J,Guo B,Wang C,Zhou G,Zhou Z,Zhang CY,Chen X

Matching two independent cohorts validates DPH1 as a gene responsible for autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
Human mutation36(10)1015-9(2015 Oct)
Loucks CM,Parboosingh JS,Shaheen R,Bernier FP,McLeod DR,Seidahmed MZ,Puffenberger EG,Ober C,Hegele RA,Boycott KM,Alkuraya FS,Innes AM

Loss of diphthamide pre-activates NF-?B and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor.
Proceedings of the National Academy of Sciences of the United States of America112(34)10732-7(2015 Aug)
Stahl S,da Silva Mateus Seidl AR,Ducret A,Kux van Geijtenbeek S,Michel S,Racek T,Birzele F,Haas AK,Rueger R,Gerg M,Niederfellner G,Pastan I,Brinkmann U

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DPH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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