DHX36 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX36
Entrez Gene ID	170506
Full Name	DEAH-box helicase 36
Synonyms	DDX36,G4R1,MLEL1,RHAU
General protein information	Preferred Names DEAH-box helicase 36 Names ATP-dependent RNA helicase DHX36 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 DEAH box protein 36 G4 resolvase-1 MLE-like protein 1 RNA helicase associated with AU-rich element ARE probable ATP-dependent RNA helicase DHX36
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.2
Summary	This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	612767

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114397.1	NP_001107869.1	ATP-dependent DNA/RNA helicase DHX36 isoform 2
NM_020865.3	NP_065916.2	ATP-dependent DNA/RNA helicase DHX36 isoform 1
NM_020865.2	NP_065916.2	ATP-dependent RNA helicase DHX36 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX36	NP_065916.2
Arabidopsis thaliana (thale cress)	AT2G35920	NP_850255.1
Arabidopsis thaliana (thale cress)	AT1G48650	NP_175298.2
Bos taurus (cattle)	DHX36	NP_001073720.1
Rattus norvegicus (Norway rat)	Dhx36	NP_001101148.1
Drosophila melanogaster (fruit fly)	CG9323	NP_001260639.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008239	XP_317231.4
Arabidopsis thaliana (thale cress)	AT2G01130	NP_178223.2
Xenopus tropicalis (tropical clawed frog)	dhx36	XP_002933292.1
Mus musculus (house mouse)	Dhx36	NP_082412.2
Arabidopsis thaliana (thale cress)	AT5G04895	NP_680142.2
	DHX36	XP_001106252.1
Gallus gallus (chicken)	DHX36	XP_422834.3
Danio rerio (zebrafish)	si:dkey-119o24.1	NP_001122016.1
Pan troglodytes (chimpanzee)	DHX36	XP_001147599.1
Canis lupus familiaris (dog)	DHX36	XP_534311.3

Gene Ontology
Bibliography

Related articles in PubMed

The G-quadruplex (G4) resolvase DHX36 efficiently and specifically disrupts DNA G4s via a translocation-based helicase mechanism.
Yangyuoru PM, Bradburn DA, Liu Z, Xiao TS, Russell R
The Journal of biological chemistry(2017 Dec)

RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.
You H, Lattmann S, Rhodes D, Yan J
Nucleic acids research45(1)206-214(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RHAU mediates the unfolding of DNA and RNA quadruplexes.
Title: G-quadruplexes unfolding by RHAU helicase.

The study provides the first evidence that the unfolding kinetics of a G-quadruplex can be modulated by different nucleotide-bound states of the RHAU helicase.
Title: RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.

RHAU binds to an adenosine-rich region near the 3'-end of the long non-coding RNA BC200.
Title: RNA Helicase Associated with AU-rich Element (RHAU/DHX36) Interacts with the 3'-Tail of the Long Non-coding RNA BC200 (BCYRN1).

present a model showing that a replication fork disrupting a T-loop could create a 5' quadruplex with an opened 3'tail structure that is recognized by G4R1
Title: Mutational Dissection of Telomeric DNA Binding Requirements of G4 Resolvase 1 Shows that G4-Structure and Certain 3'-Tail Sequences Are Sufficient for Tight and Complete Binding.

we identify a novel function of DHX36 to facilitate viral RNA recognition
Title: DHX36 enhances RIG-I signaling by facilitating PKR-mediated antiviral stress granule formation.

The following DHX36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27704	NM_020865.3 Latest version!	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu27703	NM_001114397.1 Latest version!	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu27704	NM_020865.2	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX36 ( NM_020865.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065916.2 Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27704
Clone ID Related Accession (Same CDS sequence)	NM_020865.3 , NM_020865.2
Accession Version	NM_020865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314435.1, BC036035.1 and AC018452.11. On Nov 22, 2018 this sequence version replaced NM_020865.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Isoform 1 localizes to the nucleus and cytoplasm. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF217190.1, SRR1660805.58346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000496811.6/ ENSP00000417078.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGA TAAATTTTTA 
ATTATACCTT TACATTCACT GATGCCTACA GTTAACCAGA CACAGGTGTT TAAAAGAACC 
CCTCCTGGTG TTCGGAAAAT AGTAATTGCT ACCAACATTG CGGAGACTAG CATTACCATA 
GATGATGTCG TTTATGTGAT AGATGGAGGA AAAATAAAAG AGACGCATTT TGATACTCAG 
AACAATATCA GTACAATGTC CGCTGAGTGG GTTAGTAAAG CTAATGCCAA ACAGAGAAAA 
GGTCGAGCTG GAAGAGTTCA ACCTGGTCAT TGCTATCATC TGTATAATGG TCTTAGAGCA 
AGTCTTCTAG ATGACTATCA ACTGCCAGAA ATTTTGAGAA CTCCTTTGGA AGAACTTTGT 
TTACAAATAA AGATTTTAAG GCTAGGTGGA ATTGCTTATT TTCTGAGTAG ATTAATGGAC 
CCACCATCAA ATGAGGCAGT GTTACTCTCC ATAAGACACC TGATGGAGCT GAACGCTTTG 
GATAAACAAG AAGAATTGAC ACCTCTTGGA GTCCACTTGG CACGATTACC CGTTGAGCCA 
CATATTGGAA AAATGATTCT TTTTGGAGCA CTGTTCTGCT GCTTAGACCC AGTACTCACT 
ATTGCTGCTA GTCTCAGTTT CAAAGATCCA TTTGTCATTC CACTGGGAAA AGAAAAGATT 
GCAGATGCAA GAAGAAAGGA ATTGGCAAAG GATACTAGAA GTGATCACTT AACAGTTGTG 
AATGCGTTTG AGGGCTGGGA AGAGGCTAGG CGACGTGGTT TCAGATACGA AAAGGACTAT 
TGCTGGGAAT ATTTTCTGTC TTCAAACACA CTGCAGATGC TGCATAACAT GAAAGGACAG 
TTTGCTGAGC ATCTTCTTGG AGCTGGATTT GTAAGCAGTA GAAATCCTAA AGATCCAGAA 
TCTAATATAA ATTCAGATAA TGAGAAGATA ATTAAAGCTG TCATCTGTGC TGGTTTATAT 
CCCAAAGTTG CTAAAATTCG ACTAAATTTG GGTAAAAAAA GAAAAATGGT AAAAGTTTAC 
ACAAAAACCG ATGGCCTGGT TGCTGTTCAT CCTAAATCTG TTAATGTGGA GCAAACAGAC 
TTTCACTACA ACTGGCTTAT CTATCACCTA AAGATGAGAA CAAGCAGTAT ATACTTGTAT 
GACTGCACAG AGGTTTCCCC ATACTGTCTC TTGTTTTTTG GAGGTGACAT TTCCATCCAG 
AAGGATAACG ATCAGGAAAC TATTGCTGTA GATGAGTGGA TTGTATTTCA GTCTCCAGCA 
AGAATTGCCC ATCTTGTTAA GGAATTAAGA AAGGAACTAG ATATTCTTCT GCAAGAGAAG 
ATTGAAAGTC CTCATCCTGT AGACTGGAAT GACACTAAAT CCAGAGACTG TGCAGTACTG 
TCAGCTATTA TAGACTTGAT CAAAACACAG GAAAAGGCAA CTCCCAGGAA CTTTCCGCCA 
CGATTCCAGG ATGGATATTA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065916.2
CDS	72..3098
Translation	MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKATPRNFPPRFQDGYYS

Target ORF information:

RefSeq Version	NM_020865.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020865.3

ORF Insert Sequence:

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGA TAAATTTTTA 
ATTATACCTT TACATTCACT GATGCCTACA GTTAACCAGA CACAGGTGTT TAAAAGAACC 
CCTCCTGGTG TTCGGAAAAT AGTAATTGCT ACCAACATTG CGGAGACTAG CATTACCATA 
GATGATGTCG TTTATGTGAT AGATGGAGGA AAAATAAAAG AGACGCATTT TGATACTCAG 
AACAATATCA GTACAATGTC CGCTGAGTGG GTTAGTAAAG CTAATGCCAA ACAGAGAAAA 
GGTCGAGCTG GAAGAGTTCA ACCTGGTCAT TGCTATCATC TGTATAATGG TCTTAGAGCA 
AGTCTTCTAG ATGACTATCA ACTGCCAGAA ATTTTGAGAA CTCCTTTGGA AGAACTTTGT 
TTACAAATAA AGATTTTAAG GCTAGGTGGA ATTGCTTATT TTCTGAGTAG ATTAATGGAC 
CCACCATCAA ATGAGGCAGT GTTACTCTCC ATAAGACACC TGATGGAGCT GAACGCTTTG 
GATAAACAAG AAGAATTGAC ACCTCTTGGA GTCCACTTGG CACGATTACC CGTTGAGCCA 
CATATTGGAA AAATGATTCT TTTTGGAGCA CTGTTCTGCT GCTTAGACCC AGTACTCACT 
ATTGCTGCTA GTCTCAGTTT CAAAGATCCA TTTGTCATTC CACTGGGAAA AGAAAAGATT 
GCAGATGCAA GAAGAAAGGA ATTGGCAAAG GATACTAGAA GTGATCACTT AACAGTTGTG 
AATGCGTTTG AGGGCTGGGA AGAGGCTAGG CGACGTGGTT TCAGATACGA AAAGGACTAT 
TGCTGGGAAT ATTTTCTGTC TTCAAACACA CTGCAGATGC TGCATAACAT GAAAGGACAG 
TTTGCTGAGC ATCTTCTTGG AGCTGGATTT GTAAGCAGTA GAAATCCTAA AGATCCAGAA 
TCTAATATAA ATTCAGATAA TGAGAAGATA ATTAAAGCTG TCATCTGTGC TGGTTTATAT 
CCCAAAGTTG CTAAAATTCG ACTAAATTTG GGTAAAAAAA GAAAAATGGT AAAAGTTTAC 
ACAAAAACCG ATGGCCTGGT TGCTGTTCAT CCTAAATCTG TTAATGTGGA GCAAACAGAC 
TTTCACTACA ACTGGCTTAT CTATCACCTA AAGATGAGAA CAAGCAGTAT ATACTTGTAT 
GACTGCACAG AGGTTTCCCC ATACTGTCTC TTGTTTTTTG GAGGTGACAT TTCCATCCAG 
AAGGATAACG ATCAGGAAAC TATTGCTGTA GATGAGTGGA TTGTATTTCA GTCTCCAGCA 
AGAATTGCCC ATCTTGTTAA GGAATTAAGA AAGGAACTAG ATATTCTTCT GCAAGAGAAG 
ATTGAAAGTC CTCATCCTGT AGACTGGAAT GACACTAAAT CCAGAGACTG TGCAGTACTG 
TCAGCTATTA TAGACTTGAT CAAAACACAG GAAAAGGCAA CTCCCAGGAA CTTTCCGCCA 
CGATTCCAGG ATGGATATTA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX36 ( NM_020865.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065916.2 Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27704
Clone ID Related Accession (Same CDS sequence)	NM_020865.3 , NM_020865.2
Accession Version	NM_020865.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX36 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314435.1 and BC036035.1. On Feb 14, 2008 this sequence version replaced NM_020865.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Isoform 1 localizes to the nucleus and cytoplasm. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314435.1, AF217190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGA TAAATTTTTA 
ATTATACCTT TACATTCACT GATGCCTACA GTTAACCAGA CACAGGTGTT TAAAAGAACC 
CCTCCTGGTG TTCGGAAAAT AGTAATTGCT ACCAACATTG CGGAGACTAG CATTACCATA 
GATGATGTCG TTTATGTGAT AGATGGAGGA AAAATAAAAG AGACGCATTT TGATACTCAG 
AACAATATCA GTACAATGTC CGCTGAGTGG GTTAGTAAAG CTAATGCCAA ACAGAGAAAA 
GGTCGAGCTG GAAGAGTTCA ACCTGGTCAT TGCTATCATC TGTATAATGG TCTTAGAGCA 
AGTCTTCTAG ATGACTATCA ACTGCCAGAA ATTTTGAGAA CTCCTTTGGA AGAACTTTGT 
TTACAAATAA AGATTTTAAG GCTAGGTGGA ATTGCTTATT TTCTGAGTAG ATTAATGGAC 
CCACCATCAA ATGAGGCAGT GTTACTCTCC ATAAGACACC TGATGGAGCT GAACGCTTTG 
GATAAACAAG AAGAATTGAC ACCTCTTGGA GTCCACTTGG CACGATTACC CGTTGAGCCA 
CATATTGGAA AAATGATTCT TTTTGGAGCA CTGTTCTGCT GCTTAGACCC AGTACTCACT 
ATTGCTGCTA GTCTCAGTTT CAAAGATCCA TTTGTCATTC CACTGGGAAA AGAAAAGATT 
GCAGATGCAA GAAGAAAGGA ATTGGCAAAG GATACTAGAA GTGATCACTT AACAGTTGTG 
AATGCGTTTG AGGGCTGGGA AGAGGCTAGG CGACGTGGTT TCAGATACGA AAAGGACTAT 
TGCTGGGAAT ATTTTCTGTC TTCAAACACA CTGCAGATGC TGCATAACAT GAAAGGACAG 
TTTGCTGAGC ATCTTCTTGG AGCTGGATTT GTAAGCAGTA GAAATCCTAA AGATCCAGAA 
TCTAATATAA ATTCAGATAA TGAGAAGATA ATTAAAGCTG TCATCTGTGC TGGTTTATAT 
CCCAAAGTTG CTAAAATTCG ACTAAATTTG GGTAAAAAAA GAAAAATGGT AAAAGTTTAC 
ACAAAAACCG ATGGCCTGGT TGCTGTTCAT CCTAAATCTG TTAATGTGGA GCAAACAGAC 
TTTCACTACA ACTGGCTTAT CTATCACCTA AAGATGAGAA CAAGCAGTAT ATACTTGTAT 
GACTGCACAG AGGTTTCCCC ATACTGTCTC TTGTTTTTTG GAGGTGACAT TTCCATCCAG 
AAGGATAACG ATCAGGAAAC TATTGCTGTA GATGAGTGGA TTGTATTTCA GTCTCCAGCA 
AGAATTGCCC ATCTTGTTAA GGAATTAAGA AAGGAACTAG ATATTCTTCT GCAAGAGAAG 
ATTGAAAGTC CTCATCCTGT AGACTGGAAT GACACTAAAT CCAGAGACTG TGCAGTACTG 
TCAGCTATTA TAGACTTGAT CAAAACACAG GAAAAGGCAA CTCCCAGGAA CTTTCCGCCA 
CGATTCCAGG ATGGATATTA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065916.2
CDS	82..3108
Translation	MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKATPRNFPPRFQDGYYS

Target ORF information:

RefSeq Version	NM_020865.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020865.2

ORF Insert Sequence:

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGA TAAATTTTTA 
ATTATACCTT TACATTCACT GATGCCTACA GTTAACCAGA CACAGGTGTT TAAAAGAACC 
CCTCCTGGTG TTCGGAAAAT AGTAATTGCT ACCAACATTG CGGAGACTAG CATTACCATA 
GATGATGTCG TTTATGTGAT AGATGGAGGA AAAATAAAAG AGACGCATTT TGATACTCAG 
AACAATATCA GTACAATGTC CGCTGAGTGG GTTAGTAAAG CTAATGCCAA ACAGAGAAAA 
GGTCGAGCTG GAAGAGTTCA ACCTGGTCAT TGCTATCATC TGTATAATGG TCTTAGAGCA 
AGTCTTCTAG ATGACTATCA ACTGCCAGAA ATTTTGAGAA CTCCTTTGGA AGAACTTTGT 
TTACAAATAA AGATTTTAAG GCTAGGTGGA ATTGCTTATT TTCTGAGTAG ATTAATGGAC 
CCACCATCAA ATGAGGCAGT GTTACTCTCC ATAAGACACC TGATGGAGCT GAACGCTTTG 
GATAAACAAG AAGAATTGAC ACCTCTTGGA GTCCACTTGG CACGATTACC CGTTGAGCCA 
CATATTGGAA AAATGATTCT TTTTGGAGCA CTGTTCTGCT GCTTAGACCC AGTACTCACT 
ATTGCTGCTA GTCTCAGTTT CAAAGATCCA TTTGTCATTC CACTGGGAAA AGAAAAGATT 
GCAGATGCAA GAAGAAAGGA ATTGGCAAAG GATACTAGAA GTGATCACTT AACAGTTGTG 
AATGCGTTTG AGGGCTGGGA AGAGGCTAGG CGACGTGGTT TCAGATACGA AAAGGACTAT 
TGCTGGGAAT ATTTTCTGTC TTCAAACACA CTGCAGATGC TGCATAACAT GAAAGGACAG 
TTTGCTGAGC ATCTTCTTGG AGCTGGATTT GTAAGCAGTA GAAATCCTAA AGATCCAGAA 
TCTAATATAA ATTCAGATAA TGAGAAGATA ATTAAAGCTG TCATCTGTGC TGGTTTATAT 
CCCAAAGTTG CTAAAATTCG ACTAAATTTG GGTAAAAAAA GAAAAATGGT AAAAGTTTAC 
ACAAAAACCG ATGGCCTGGT TGCTGTTCAT CCTAAATCTG TTAATGTGGA GCAAACAGAC 
TTTCACTACA ACTGGCTTAT CTATCACCTA AAGATGAGAA CAAGCAGTAT ATACTTGTAT 
GACTGCACAG AGGTTTCCCC ATACTGTCTC TTGTTTTTTG GAGGTGACAT TTCCATCCAG 
AAGGATAACG ATCAGGAAAC TATTGCTGTA GATGAGTGGA TTGTATTTCA GTCTCCAGCA 
AGAATTGCCC ATCTTGTTAA GGAATTAAGA AAGGAACTAG ATATTCTTCT GCAAGAGAAG 
ATTGAAAGTC CTCATCCTGT AGACTGGAAT GACACTAAAT CCAGAGACTG TGCAGTACTG 
TCAGCTATTA TAGACTTGAT CAAAACACAG GAAAAGGCAA CTCCCAGGAA CTTTCCGCCA 
CGATTCCAGG ATGGATATTA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX36 ( NM_001114397.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107869.1 Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 2, mRNA.

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CloneID	OHu27703
Clone ID Related Accession (Same CDS sequence)	NM_001114397.1
Accession Version	NM_001114397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314435.1, AJ577134.1 and BC036035.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1, resulting in a shorter protein (isoform 2). Isoform 2 localizes to the cytoplasm. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ577134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGT TAACCAGACA 
CAGGTGTTTA AAAGAACCCC TCCTGGTGTT CGGAAAATAG TAATTGCTAC CAACATTGCG 
GAGACTAGCA TTACCATAGA TGATGTCGTT TATGTGATAG ATGGAGGAAA AATAAAAGAG 
ACGCATTTTG ATACTCAGAA CAATATCAGT ACAATGTCCG CTGAGTGGGT TAGTAAAGCT 
AATGCCAAAC AGAGAAAAGG TCGAGCTGGA AGAGTTCAAC CTGGTCATTG CTATCATCTG 
TATAATGGTC TTAGAGCAAG TCTTCTAGAT GACTATCAAC TGCCAGAAAT TTTGAGAACT 
CCTTTGGAAG AACTTTGTTT ACAAATAAAG ATTTTAAGGC TAGGTGGAAT TGCTTATTTT 
CTGAGTAGAT TAATGGACCC ACCATCAAAT GAGGCAGTGT TACTCTCCAT AAGACACCTG 
ATGGAGCTGA ACGCTTTGGA TAAACAAGAA GAATTGACAC CTCTTGGAGT CCACTTGGCA 
CGATTACCCG TTGAGCCACA TATTGGAAAA ATGATTCTTT TTGGAGCACT GTTCTGCTGC 
TTAGACCCAG TACTCACTAT TGCTGCTAGT CTCAGTTTCA AAGATCCATT TGTCATTCCA 
CTGGGAAAAG AAAAGATTGC AGATGCAAGA AGAAAGGAAT TGGCAAAGGA TACTAGAAGT 
GATCACTTAA CAGTTGTGAA TGCGTTTGAG GGCTGGGAAG AGGCTAGGCG ACGTGGTTTC 
AGATACGAAA AGGACTATTG CTGGGAATAT TTTCTGTCTT CAAACACACT GCAGATGCTG 
CATAACATGA AAGGACAGTT TGCTGAGCAT CTTCTTGGAG CTGGATTTGT AAGCAGTAGA 
AATCCTAAAG ATCCAGAATC TAATATAAAT TCAGATAATG AGAAGATAAT TAAAGCTGTC 
ATCTGTGCTG GTTTATATCC CAAAGTTGCT AAAATTCGAC TAAATTTGGG TAAAAAAAGA 
AAAATGGTAA AAGTTTACAC AAAAACCGAT GGCCTGGTTG CTGTTCATCC TAAATCTGTT 
AATGTGGAGC AAACAGACTT TCACTACAAC TGGCTTATCT ATCACCTAAA GATGAGAACA 
AGCAGTATAT ACTTGTATGA CTGCACAGAG GTTTCCCCAT ACTGTCTCTT GTTTTTTGGA 
GGTGACATTT CCATCCAGAA GGATAACGAT CAGGAAACTA TTGCTGTAGA TGAGTGGATT 
GTATTTCAGT CTCCAGCAAG AATTGCCCAT CTTGTTAAGG AATTAAGAAA GGAACTAGAT 
ATTCTTCTGC AAGAGAAGAT TGAAAGTCCT CATCCTGTAG ACTGGAATGA CACTAAATCC 
AGAGACTGTG CAGTACTGTC AGCTATTATA GACTTGATCA AAACACAGGA AAAGGCAACT 
CCCAGGAACT TTCCGCCACG ATTCCAGGAT GGATATTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107869.1
CDS	82..3066
Translation	MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKATPRNFPPRFQDGYYS

Target ORF information:

RefSeq Version	NM_001114397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114397.1

ORF Insert Sequence:

ATGAGTTATG ACTACCATCA GAACTGGGGC CGTGATGGGG GTCCCCGCAG CTCCGGTGGG 
GGCTATGGAG GGGGGCCAGC AGGGGGTCAT GGAGGTAACC GAGGCTCCGG AGGAGGCGGC 
GGCGGCGGAG GGGGTGGTCG AGGCGGCAGG GGCCGGCATC CCGGGCACCT GAAAGGCCGC 
GAAATCGGCA TGTGGTACGC GAAAAAACAG GGGCAGAAGA ACAAGGAAGC GGAGAGGCAA 
GAGAGAGCTG TAGTACACAT GGATGAACGA CGAGAAGAAC AAATTGTACA GTTACTGAAT 
TCTGTTCAAG CGAAGAATGA TAAAGAGTCA GAAGCACAGA TATCCTGGTT TGCTCCTGAG 
GATCATGGAT ACGGTACTGA AGTTTCTACT AAGAACACAC CATGCTCAGA GAACAAACTT 
GACATCCAGG AAAAGAAGTT GATAAATCAA GAAAAAAAAA TGTTTAGAAT CAGGAACAGA 
TCATATATTG ACCGAGATTC TGAGTATCTC TTGCAAGAAA ATGAACCAGA TGGAACTTTA 
GACCAAAAAT TATTGGAAGA TTTACAAAAG AAAAAAAATG ACCTTCGGTA TATTGAAATG 
CAGCATTTCA GAGAAAAGCT GCCTTCGTAT GGAATGCAAA AGGAATTGGT AAATTTAATT 
GATAACCATC AGGTAACAGT AATAAGTGGT GAAACTGGTT GTGGCAAAAC CACTCAAGTT 
ACTCAGTTCA TTTTGGATAA CTACATTGAA AGAGGAAAAG GATCTGCTTG CAGAATAGTT 
TGTACTCAGC CAAGAAGAAT TAGTGCCATT TCAGTTGCGG AAAGAGTAGC TGCAGAAAGG 
GCAGAATCTT GTGGCAGTGG TAATAGTACT GGATATCAAA TTCGTCTCCA GAGTCGGTTG 
CCAAGGAAAC AGGGTTCTAT CTTATACTGT ACAACAGGAA TCATCCTTCA GTGGCTCCAG 
TCAGACCCGT ATTTGTCCAG TGTTAGTCAT ATCGTACTTG ATGAAATCCA TGAAAGAAAT 
CTGCAGTCAG ATGTTTTAAT GACTGTTGTT AAAGACCTTC TCAATTTTCG ATCTGACTTG 
AAAGTAATAT TGATGAGTGC AACATTGAAT GCAGAAAAGT TTTCAGAATA TTTTGGTAAC 
TGTCCAATGA TACATATACC TGGTTTTACC TTTCCGGTTG TGGAATATCT TTTGGAAGAT 
GTAATTGAAA AAATAAGGTA TGTTCCAGAA CAAAAAGAAC ACAGATCCCA GTTTAAGAGG 
GGTTTCATGC AAGGGCATGT AAATAGACAA GAAAAAGAAG AAAAAGAAGC AATATATAAA 
GAACGTTGGC CAGATTATGT AAGGGAACTG CGAAGAAGGT ATTCTGCAAG TACTGTAGAT 
GTTATAGAAA TGATGGAGGA TGATAAAGTT GATCTGAATT TGATTGTTGC CCTCATCCGA 
TACATTGTTT TGGAAGAAGA GGATGGTGCG ATACTGGTCT TTCTGCCAGG CTGGGACAAT 
ATCAGCACTT TACATGATCT CTTGATGTCA CAAGTAATGT TTAAATCAGT TAACCAGACA 
CAGGTGTTTA AAAGAACCCC TCCTGGTGTT CGGAAAATAG TAATTGCTAC CAACATTGCG 
GAGACTAGCA TTACCATAGA TGATGTCGTT TATGTGATAG ATGGAGGAAA AATAAAAGAG 
ACGCATTTTG ATACTCAGAA CAATATCAGT ACAATGTCCG CTGAGTGGGT TAGTAAAGCT 
AATGCCAAAC AGAGAAAAGG TCGAGCTGGA AGAGTTCAAC CTGGTCATTG CTATCATCTG 
TATAATGGTC TTAGAGCAAG TCTTCTAGAT GACTATCAAC TGCCAGAAAT TTTGAGAACT 
CCTTTGGAAG AACTTTGTTT ACAAATAAAG ATTTTAAGGC TAGGTGGAAT TGCTTATTTT 
CTGAGTAGAT TAATGGACCC ACCATCAAAT GAGGCAGTGT TACTCTCCAT AAGACACCTG 
ATGGAGCTGA ACGCTTTGGA TAAACAAGAA GAATTGACAC CTCTTGGAGT CCACTTGGCA 
CGATTACCCG TTGAGCCACA TATTGGAAAA ATGATTCTTT TTGGAGCACT GTTCTGCTGC 
TTAGACCCAG TACTCACTAT TGCTGCTAGT CTCAGTTTCA AAGATCCATT TGTCATTCCA 
CTGGGAAAAG AAAAGATTGC AGATGCAAGA AGAAAGGAAT TGGCAAAGGA TACTAGAAGT 
GATCACTTAA CAGTTGTGAA TGCGTTTGAG GGCTGGGAAG AGGCTAGGCG ACGTGGTTTC 
AGATACGAAA AGGACTATTG CTGGGAATAT TTTCTGTCTT CAAACACACT GCAGATGCTG 
CATAACATGA AAGGACAGTT TGCTGAGCAT CTTCTTGGAG CTGGATTTGT AAGCAGTAGA 
AATCCTAAAG ATCCAGAATC TAATATAAAT TCAGATAATG AGAAGATAAT TAAAGCTGTC 
ATCTGTGCTG GTTTATATCC CAAAGTTGCT AAAATTCGAC TAAATTTGGG TAAAAAAAGA 
AAAATGGTAA AAGTTTACAC AAAAACCGAT GGCCTGGTTG CTGTTCATCC TAAATCTGTT 
AATGTGGAGC AAACAGACTT TCACTACAAC TGGCTTATCT ATCACCTAAA GATGAGAACA 
AGCAGTATAT ACTTGTATGA CTGCACAGAG GTTTCCCCAT ACTGTCTCTT GTTTTTTGGA 
GGTGACATTT CCATCCAGAA GGATAACGAT CAGGAAACTA TTGCTGTAGA TGAGTGGATT 
GTATTTCAGT CTCCAGCAAG AATTGCCCAT CTTGTTAAGG AATTAAGAAA GGAACTAGAT 
ATTCTTCTGC AAGAGAAGAT TGAAAGTCCT CATCCTGTAG ACTGGAATGA CACTAAATCC 
AGAGACTGTG CAGTACTGTC AGCTATTATA GACTTGATCA AAACACAGGA AAAGGCAACT 
CCCAGGAACT TTCCGCCACG ATTCCAGGAT GGATATTACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The G-quadruplex (G4) resolvase DHX36 efficiently and specifically disrupts DNA G4s via a translocation-based helicase mechanism.
The Journal of biological chemistry(2017 Dec)
Yangyuoru PM,Bradburn DA,Liu Z,Xiao TS,Russell R

RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.
Nucleic acids research45(1)206-214(2017 Jan)
You H,Lattmann S,Rhodes D,Yan J

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DHX36 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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