DDX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX1
Entrez Gene ID	1653
Full Name	DEAD-box helicase 1
Synonyms	DBP-RB,UKVH5d
General protein information	Preferred Names DEAD-box helicase 1 Names ATP-dependent RNA helicase DDX1 DEAD (Asp-Glu-Ala-Asp) box helicase 1 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 DEAD box polypeptide 1 DEAD box protein 1 DEAD box protein retinoblastoma DEAD box-1 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1 DEAD/H-box helicase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p24.3
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008].
Disorder MIM:	601257

mRNA and Protein(s)

mRNA	Protein	Name
NM_004939.3	NP_004930.1	ATP-dependent RNA helicase DDX1
NM_004939.2	NP_004930.1	ATP-dependent RNA helicase DDX1

Pathways from BioSystems

Homologies

	DDX1	XP_001090643.1
Homo sapiens (human)	DDX1	NP_004930.1
Drosophila melanogaster (fruit fly)	Ddx1	NP_524212.2
Xenopus tropicalis (tropical clawed frog)	ddx1	NP_001011133.1
Rattus norvegicus (Norway rat)	Ddx1	NP_445866.1
Gallus gallus (chicken)	DDX1	NP_989894.1
Canis lupus familiaris (dog)	DDX1	XP_848865.1
Bos taurus (cattle)	DDX1	NP_001068936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004765	XP_318051.4
Caenorhabditis elegans (roundworm)	Y55F3BR.1	NP_500004.2
Pan troglodytes (chimpanzee)	DDX1	XP_001162067.2
Mus musculus (house mouse)	Ddx1	NP_598801.1
Danio rerio (zebrafish)	ddx1	NP_001184253.1

Gene Ontology
Bibliography

Related articles in PubMed

Role for RIF1-interacting partner DDX1 in BLM recruitment to DNA double-strand breaks.
Li L, Poon HY, Hildebrandt MR, Monckton EA, Germain DR, Fahlman RP, Godbout R
DNA repair5547-63(2017 07)

A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly.
Lamichhane R, Hammond JA, Pauszek RF 3rd, Anderson RM, Pedron I, van der Schans E, Williamson JR, Millar DP
Nucleic acids research45(8)4632-4641(2017 May)

FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.
Akter KA, Mansour MA, Hyodo T, Senga T
The international journal of biochemistry & cell biology841-13(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDX1, a RNA helicase also implicated in DSB repair, interacts with RIF1. Recruitment of DDX1 to DSBs is dependent on RIF1. DDX1 is also required for chromatin loading of BLM to ionizing radiation-induced DSBs. DDX1 and RIF1 have different nucleic acid requirements for accumulation at DSBs, with RNA-DNA hybrids required for DDX1 accrual at DSBs, and single-strand RNA required for accumulation of RIF1 at these sites.
Title: Role for RIF1-interacting partner DDX1 in BLM recruitment to DNA double-strand breaks.

DDX1 binds HIV-1 rev response element to promote virus assembly.
Title: A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly.

the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression in colorectal cancer cell lines
Title: FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.

Data provide evidence for a role for DDX1 in resolving RNA-DNA structures that accumulate at DNA double-strand breaks located at sites of active transcription thereby facilitating repair by homologous recombination.
Title: DEAD Box 1 Facilitates Removal of RNA and Homologous Recombination at DNA Double-Strand Breaks.

data have demonstrated that Venezuelan equine encephalitis virus (VEEV)-nsP3 associates with DDX1 and DDX3 in infected cells to facilitate viral multiplication; determined that VEEV-nsP3 interacts with a pre-formed translational complex, comprising of DX3:eIF4A:eIF4G:PABP to potentially aid in translation of viral proteins.
Title: Venezuelan equine encephalitis virus non-structural protein 3 (nsP3) interacts with RNA helicases DDX1 and DDX3 in infected cells.

The following DDX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15746	NM_004939.3 Latest version!	Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu15746	NM_004939.2	Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX1 ( NM_004939.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004930.1 Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.

Price & Availability↑

CloneID	OHu15746
Clone ID Related Accession (Same CDS sequence)	NM_004939.3 , NM_004939.2
Accession Version	NM_004939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X70649.1 and BC053673.1. On Nov 22, 2018 this sequence version replaced NM_004939.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X70649.1, SRR1803614.49929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233084.8/ ENSP00000233084.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT TCTCCGAGAT GGGTGTAATG CCTGAGATTG CACAAGCTGT GGAAGAGATG 
GATTGGCTCC TCCCAACTGA TATCCAGGCT GAATCTATCC CATTGATCTT AGGAGGAGGT 
GATGTACTTA TGGCTGCAGA AACAGGAAGT GGCAAAACTG GTGCTTTTAG TATTCCAGTT 
ATCCAGATAG TTTATGAAAC TCTGAAAGAC CAACAGGAAG GCAAAAAAGG AAAAACAACA 
ATTAAAACTG GTGCTTCAGT GCTGAACAAA TGGCAGATGA ACCCATATGA CAGAGGATCT 
GCTTTTGCAA TTGGGTCAGA TGGTCTTTGT TGTCAAAGCA GAGAAGTAAA GGAATGGCAT 
GGGTGTAGAG CTACTAAAGG ATTAATGAAA GGGAAACACT ACTATGAAGT ATCCTGTCAT 
GACCAAGGGT TATGCAGGGT CGGGTGGTCT ACCATGCAGG CCTCTTTGGA CCTAGGTACT 
GACAAGTTTG GATTTGGCTT TGGTGGAACA GGAAAGAAAT CCCATAACAA ACAATTTGAT 
AATTATGGAG AGGAATTCAC TATGCATGAT ACCATTGGAT GTTACCTGGA TATAGATAAG 
GGACATGTCA AGTTCTCCAA AAATGGAAAA GATCTTGGTC TGGCATTTGA AATACCACCA 
CATATGAAAA ACCAAGCCCT CTTTCCTGCC TGTGTTTTGA AGAATGCTGA ACTGAAATTT 
AACTTCGGTG AAGAGGAATT TAAGTTTCCA CCAAAAGATG GCTTTGTTGC TCTTTCCAAG 
GCACCGGATG GTTACATTGT CAAATCACAG CACTCAGGTA ATGCACAGGT GACACAAACA 
AAGTTTCTCC CCAATGCTCC GAAAGCTCTC ATTGTTGAAC CTTCCCGGGA GTTAGCTGAA 
CAAACTTTGA ACAACATCAA GCAGTTTAAG AAATACATTG ATAATCCTAA ATTAAGGGAG 
CTTCTGATAA TTGGAGGTGT TGCAGCCCGG GATCAGCTCT CTGTTTTGGA AAATGGAGTA 
GATATAGTTG TAGGTACTCC GGGAAGACTA GATGACTTGG TGTCAACTGG AAAGCTGAAC 
TTATCTCAAG TTAGATTCCT GGTCCTGGAT GAAGCTGATG GGCTTCTTTC TCAAGGTTAT 
TCTGATTTTA TAAATAGGAT GCACAATCAG ATTCCTCAGG TTACCTCTGA TGGAAAAAGA 
CTTCAGGTGA TTGTTTGCTC TGCCACTTTG CATTCTTTCG ATGTAAAGAA ACTGTCCGAG 
AAGATAATGC ATTTTCCTAC ATGGGTTGAC TTAAAAGGAG AAGACTCTGT TCCAGATACT 
GTACACCATG TTGTTGTCCC AGTAAATCCC AAAACTGACA GACTCTGGGA AAGGCTTGGA 
AAGAGCCACA TTAGAACTGA TGATGTACAT GCAAAAGATA ACACAAGACC TGGTGCTAAT 
AGTCCAGAGA TGTGGTCTGA AGCTATTAAA ATCCTGAAAG GGGAGTATGC TGTCCGGGCA 
ATCAAGGAAC ATAAGATGGA TCAAGCAATT ATCTTCTGTA GAACCAAAAT TGACTGTGAT 
AACTTGGAGC AGTACTTTAT ACAACAAGGA GGAGGACCTG ATAAAAAAGG ACACCAGTTC 
TCATGTGTTT GTCTTCATGG TGACAGAAAG CCTCATGAGA GAAAGCAAAA CTTGGAAAGA 
TTTAAGAAAG GAGATGTAAG ATTCTTGATT TGCACAGATG TAGCTGCTAG AGGAATTGAT 
ATCCACGGTG TTCCTTATGT TATAAATGTC ACTCTGCCCG ATGAAAAGCA AAACTACGTA 
CATCGAATTG GCAGAGTAGG AAGAGCTGAA AGGATGGGTC TGGCAATTTC CCTGGTGGCA 
ACAGAAAAAG AAAAGGTTTG GTACCATGTA TGTAGCAGCC GTGGAAAAGG GTGTTATAAC 
ACAAGACTCA AGGAAGATGG AGGCTGTACC ATATGGTACA ACGAGATGCA GTTACTATCT 
GAGATAGAAG AACACCTGAA CTGTACCATT TCTCAGGTTG AGCCGGATAT AAAGGTACCA 
GTGGATGAAT TTGATGGGAA AGTTACCTAC GGTCAGAAAA GGGCTGCTGG TGGTGGAAGC 
TATAAAGGCC ATGTGGATAT TTTGGCACCT ACTGTTCAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCGCAGA CATCTTTCCT GCATCTTGGC TACCTTCCTA ACCAGCTGTT CAGAACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004930.1
CDS	67..2289
Translation	MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

Target ORF information:

RefSeq Version	NM_004939.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004939.3

ORF Insert Sequence:

ATGGCGGCCT TCTCCGAGAT GGGTGTAATG CCTGAGATTG CACAAGCTGT GGAAGAGATG 
GATTGGCTCC TCCCAACTGA TATCCAGGCT GAATCTATCC CATTGATCTT AGGAGGAGGT 
GATGTACTTA TGGCTGCAGA AACAGGAAGT GGCAAAACTG GTGCTTTTAG TATTCCAGTT 
ATCCAGATAG TTTATGAAAC TCTGAAAGAC CAACAGGAAG GCAAAAAAGG AAAAACAACA 
ATTAAAACTG GTGCTTCAGT GCTGAACAAA TGGCAGATGA ACCCATATGA CAGAGGATCT 
GCTTTTGCAA TTGGGTCAGA TGGTCTTTGT TGTCAAAGCA GAGAAGTAAA GGAATGGCAT 
GGGTGTAGAG CTACTAAAGG ATTAATGAAA GGGAAACACT ACTATGAAGT ATCCTGTCAT 
GACCAAGGGT TATGCAGGGT CGGGTGGTCT ACCATGCAGG CCTCTTTGGA CCTAGGTACT 
GACAAGTTTG GATTTGGCTT TGGTGGAACA GGAAAGAAAT CCCATAACAA ACAATTTGAT 
AATTATGGAG AGGAATTCAC TATGCATGAT ACCATTGGAT GTTACCTGGA TATAGATAAG 
GGACATGTCA AGTTCTCCAA AAATGGAAAA GATCTTGGTC TGGCATTTGA AATACCACCA 
CATATGAAAA ACCAAGCCCT CTTTCCTGCC TGTGTTTTGA AGAATGCTGA ACTGAAATTT 
AACTTCGGTG AAGAGGAATT TAAGTTTCCA CCAAAAGATG GCTTTGTTGC TCTTTCCAAG 
GCACCGGATG GTTACATTGT CAAATCACAG CACTCAGGTA ATGCACAGGT GACACAAACA 
AAGTTTCTCC CCAATGCTCC GAAAGCTCTC ATTGTTGAAC CTTCCCGGGA GTTAGCTGAA 
CAAACTTTGA ACAACATCAA GCAGTTTAAG AAATACATTG ATAATCCTAA ATTAAGGGAG 
CTTCTGATAA TTGGAGGTGT TGCAGCCCGG GATCAGCTCT CTGTTTTGGA AAATGGAGTA 
GATATAGTTG TAGGTACTCC GGGAAGACTA GATGACTTGG TGTCAACTGG AAAGCTGAAC 
TTATCTCAAG TTAGATTCCT GGTCCTGGAT GAAGCTGATG GGCTTCTTTC TCAAGGTTAT 
TCTGATTTTA TAAATAGGAT GCACAATCAG ATTCCTCAGG TTACCTCTGA TGGAAAAAGA 
CTTCAGGTGA TTGTTTGCTC TGCCACTTTG CATTCTTTCG ATGTAAAGAA ACTGTCCGAG 
AAGATAATGC ATTTTCCTAC ATGGGTTGAC TTAAAAGGAG AAGACTCTGT TCCAGATACT 
GTACACCATG TTGTTGTCCC AGTAAATCCC AAAACTGACA GACTCTGGGA AAGGCTTGGA 
AAGAGCCACA TTAGAACTGA TGATGTACAT GCAAAAGATA ACACAAGACC TGGTGCTAAT 
AGTCCAGAGA TGTGGTCTGA AGCTATTAAA ATCCTGAAAG GGGAGTATGC TGTCCGGGCA 
ATCAAGGAAC ATAAGATGGA TCAAGCAATT ATCTTCTGTA GAACCAAAAT TGACTGTGAT 
AACTTGGAGC AGTACTTTAT ACAACAAGGA GGAGGACCTG ATAAAAAAGG ACACCAGTTC 
TCATGTGTTT GTCTTCATGG TGACAGAAAG CCTCATGAGA GAAAGCAAAA CTTGGAAAGA 
TTTAAGAAAG GAGATGTAAG ATTCTTGATT TGCACAGATG TAGCTGCTAG AGGAATTGAT 
ATCCACGGTG TTCCTTATGT TATAAATGTC ACTCTGCCCG ATGAAAAGCA AAACTACGTA 
CATCGAATTG GCAGAGTAGG AAGAGCTGAA AGGATGGGTC TGGCAATTTC CCTGGTGGCA 
ACAGAAAAAG AAAAGGTTTG GTACCATGTA TGTAGCAGCC GTGGAAAAGG GTGTTATAAC 
ACAAGACTCA AGGAAGATGG AGGCTGTACC ATATGGTACA ACGAGATGCA GTTACTATCT 
GAGATAGAAG AACACCTGAA CTGTACCATT TCTCAGGTTG AGCCGGATAT AAAGGTACCA 
GTGGATGAAT TTGATGGGAA AGTTACCTAC GGTCAGAAAA GGGCTGCTGG TGGTGGAAGC 
TATAAAGGCC ATGTGGATAT TTTGGCACCT ACTGTTCAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCGCAGA CATCTTTCCT GCATCTTGGC TACCTTCCTA ACCAGCTGTT CAGAACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX1 ( NM_004939.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004930.1 Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.

Price & Availability↑

CloneID	OHu15746
Clone ID Related Accession (Same CDS sequence)	NM_004939.3 , NM_004939.2
Accession Version	NM_004939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC360930.1, X70649.1 and BC053673.1. On Mar 7, 2012 this sequence version replaced NM_004939.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053673.1, X70649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT TCTCCGAGAT GGGTGTAATG CCTGAGATTG CACAAGCTGT GGAAGAGATG 
GATTGGCTCC TCCCAACTGA TATCCAGGCT GAATCTATCC CATTGATCTT AGGAGGAGGT 
GATGTACTTA TGGCTGCAGA AACAGGAAGT GGCAAAACTG GTGCTTTTAG TATTCCAGTT 
ATCCAGATAG TTTATGAAAC TCTGAAAGAC CAACAGGAAG GCAAAAAAGG AAAAACAACA 
ATTAAAACTG GTGCTTCAGT GCTGAACAAA TGGCAGATGA ACCCATATGA CAGAGGATCT 
GCTTTTGCAA TTGGGTCAGA TGGTCTTTGT TGTCAAAGCA GAGAAGTAAA GGAATGGCAT 
GGGTGTAGAG CTACTAAAGG ATTAATGAAA GGGAAACACT ACTATGAAGT ATCCTGTCAT 
GACCAAGGGT TATGCAGGGT CGGGTGGTCT ACCATGCAGG CCTCTTTGGA CCTAGGTACT 
GACAAGTTTG GATTTGGCTT TGGTGGAACA GGAAAGAAAT CCCATAACAA ACAATTTGAT 
AATTATGGAG AGGAATTCAC TATGCATGAT ACCATTGGAT GTTACCTGGA TATAGATAAG 
GGACATGTCA AGTTCTCCAA AAATGGAAAA GATCTTGGTC TGGCATTTGA AATACCACCA 
CATATGAAAA ACCAAGCCCT CTTTCCTGCC TGTGTTTTGA AGAATGCTGA ACTGAAATTT 
AACTTCGGTG AAGAGGAATT TAAGTTTCCA CCAAAAGATG GCTTTGTTGC TCTTTCCAAG 
GCACCGGATG GTTACATTGT CAAATCACAG CACTCAGGTA ATGCACAGGT GACACAAACA 
AAGTTTCTCC CCAATGCTCC GAAAGCTCTC ATTGTTGAAC CTTCCCGGGA GTTAGCTGAA 
CAAACTTTGA ACAACATCAA GCAGTTTAAG AAATACATTG ATAATCCTAA ATTAAGGGAG 
CTTCTGATAA TTGGAGGTGT TGCAGCCCGG GATCAGCTCT CTGTTTTGGA AAATGGAGTA 
GATATAGTTG TAGGTACTCC GGGAAGACTA GATGACTTGG TGTCAACTGG AAAGCTGAAC 
TTATCTCAAG TTAGATTCCT GGTCCTGGAT GAAGCTGATG GGCTTCTTTC TCAAGGTTAT 
TCTGATTTTA TAAATAGGAT GCACAATCAG ATTCCTCAGG TTACCTCTGA TGGAAAAAGA 
CTTCAGGTGA TTGTTTGCTC TGCCACTTTG CATTCTTTCG ATGTAAAGAA ACTGTCCGAG 
AAGATAATGC ATTTTCCTAC ATGGGTTGAC TTAAAAGGAG AAGACTCTGT TCCAGATACT 
GTACACCATG TTGTTGTCCC AGTAAATCCC AAAACTGACA GACTCTGGGA AAGGCTTGGA 
AAGAGCCACA TTAGAACTGA TGATGTACAT GCAAAAGATA ACACAAGACC TGGTGCTAAT 
AGTCCAGAGA TGTGGTCTGA AGCTATTAAA ATCCTGAAAG GGGAGTATGC TGTCCGGGCA 
ATCAAGGAAC ATAAGATGGA TCAAGCAATT ATCTTCTGTA GAACCAAAAT TGACTGTGAT 
AACTTGGAGC AGTACTTTAT ACAACAAGGA GGAGGACCTG ATAAAAAAGG ACACCAGTTC 
TCATGTGTTT GTCTTCATGG TGACAGAAAG CCTCATGAGA GAAAGCAAAA CTTGGAAAGA 
TTTAAGAAAG GAGATGTAAG ATTCTTGATT TGCACAGATG TAGCTGCTAG AGGAATTGAT 
ATCCACGGTG TTCCTTATGT TATAAATGTC ACTCTGCCCG ATGAAAAGCA AAACTACGTA 
CATCGAATTG GCAGAGTAGG AAGAGCTGAA AGGATGGGTC TGGCAATTTC CCTGGTGGCA 
ACAGAAAAAG AAAAGGTTTG GTACCATGTA TGTAGCAGCC GTGGAAAAGG GTGTTATAAC 
ACAAGACTCA AGGAAGATGG AGGCTGTACC ATATGGTACA ACGAGATGCA GTTACTATCT 
GAGATAGAAG AACACCTGAA CTGTACCATT TCTCAGGTTG AGCCGGATAT AAAGGTACCA 
GTGGATGAAT TTGATGGGAA AGTTACCTAC GGTCAGAAAA GGGCTGCTGG TGGTGGAAGC 
TATAAAGGCC ATGTGGATAT TTTGGCACCT ACTGTTCAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCGCAGA CATCTTTCCT GCATCTTGGC TACCTTCCTA ACCAGCTGTT CAGAACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004930.1
CDS	314..2536
Translation	MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

Target ORF information:

RefSeq Version	NM_004939.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 1 (DDX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004939.2

ORF Insert Sequence:

ATGGCGGCCT TCTCCGAGAT GGGTGTAATG CCTGAGATTG CACAAGCTGT GGAAGAGATG 
GATTGGCTCC TCCCAACTGA TATCCAGGCT GAATCTATCC CATTGATCTT AGGAGGAGGT 
GATGTACTTA TGGCTGCAGA AACAGGAAGT GGCAAAACTG GTGCTTTTAG TATTCCAGTT 
ATCCAGATAG TTTATGAAAC TCTGAAAGAC CAACAGGAAG GCAAAAAAGG AAAAACAACA 
ATTAAAACTG GTGCTTCAGT GCTGAACAAA TGGCAGATGA ACCCATATGA CAGAGGATCT 
GCTTTTGCAA TTGGGTCAGA TGGTCTTTGT TGTCAAAGCA GAGAAGTAAA GGAATGGCAT 
GGGTGTAGAG CTACTAAAGG ATTAATGAAA GGGAAACACT ACTATGAAGT ATCCTGTCAT 
GACCAAGGGT TATGCAGGGT CGGGTGGTCT ACCATGCAGG CCTCTTTGGA CCTAGGTACT 
GACAAGTTTG GATTTGGCTT TGGTGGAACA GGAAAGAAAT CCCATAACAA ACAATTTGAT 
AATTATGGAG AGGAATTCAC TATGCATGAT ACCATTGGAT GTTACCTGGA TATAGATAAG 
GGACATGTCA AGTTCTCCAA AAATGGAAAA GATCTTGGTC TGGCATTTGA AATACCACCA 
CATATGAAAA ACCAAGCCCT CTTTCCTGCC TGTGTTTTGA AGAATGCTGA ACTGAAATTT 
AACTTCGGTG AAGAGGAATT TAAGTTTCCA CCAAAAGATG GCTTTGTTGC TCTTTCCAAG 
GCACCGGATG GTTACATTGT CAAATCACAG CACTCAGGTA ATGCACAGGT GACACAAACA 
AAGTTTCTCC CCAATGCTCC GAAAGCTCTC ATTGTTGAAC CTTCCCGGGA GTTAGCTGAA 
CAAACTTTGA ACAACATCAA GCAGTTTAAG AAATACATTG ATAATCCTAA ATTAAGGGAG 
CTTCTGATAA TTGGAGGTGT TGCAGCCCGG GATCAGCTCT CTGTTTTGGA AAATGGAGTA 
GATATAGTTG TAGGTACTCC GGGAAGACTA GATGACTTGG TGTCAACTGG AAAGCTGAAC 
TTATCTCAAG TTAGATTCCT GGTCCTGGAT GAAGCTGATG GGCTTCTTTC TCAAGGTTAT 
TCTGATTTTA TAAATAGGAT GCACAATCAG ATTCCTCAGG TTACCTCTGA TGGAAAAAGA 
CTTCAGGTGA TTGTTTGCTC TGCCACTTTG CATTCTTTCG ATGTAAAGAA ACTGTCCGAG 
AAGATAATGC ATTTTCCTAC ATGGGTTGAC TTAAAAGGAG AAGACTCTGT TCCAGATACT 
GTACACCATG TTGTTGTCCC AGTAAATCCC AAAACTGACA GACTCTGGGA AAGGCTTGGA 
AAGAGCCACA TTAGAACTGA TGATGTACAT GCAAAAGATA ACACAAGACC TGGTGCTAAT 
AGTCCAGAGA TGTGGTCTGA AGCTATTAAA ATCCTGAAAG GGGAGTATGC TGTCCGGGCA 
ATCAAGGAAC ATAAGATGGA TCAAGCAATT ATCTTCTGTA GAACCAAAAT TGACTGTGAT 
AACTTGGAGC AGTACTTTAT ACAACAAGGA GGAGGACCTG ATAAAAAAGG ACACCAGTTC 
TCATGTGTTT GTCTTCATGG TGACAGAAAG CCTCATGAGA GAAAGCAAAA CTTGGAAAGA 
TTTAAGAAAG GAGATGTAAG ATTCTTGATT TGCACAGATG TAGCTGCTAG AGGAATTGAT 
ATCCACGGTG TTCCTTATGT TATAAATGTC ACTCTGCCCG ATGAAAAGCA AAACTACGTA 
CATCGAATTG GCAGAGTAGG AAGAGCTGAA AGGATGGGTC TGGCAATTTC CCTGGTGGCA 
ACAGAAAAAG AAAAGGTTTG GTACCATGTA TGTAGCAGCC GTGGAAAAGG GTGTTATAAC 
ACAAGACTCA AGGAAGATGG AGGCTGTACC ATATGGTACA ACGAGATGCA GTTACTATCT 
GAGATAGAAG AACACCTGAA CTGTACCATT TCTCAGGTTG AGCCGGATAT AAAGGTACCA 
GTGGATGAAT TTGATGGGAA AGTTACCTAC GGTCAGAAAA GGGCTGCTGG TGGTGGAAGC 
TATAAAGGCC ATGTGGATAT TTTGGCACCT ACTGTTCAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCGCAGA CATCTTTCCT GCATCTTGGC TACCTTCCTA ACCAGCTGTT CAGAACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role for RIF1-interacting partner DDX1 in BLM recruitment to DNA double-strand breaks.
DNA repair5547-63(2017 07)
Li L,Poon HY,Hildebrandt MR,Monckton EA,Germain DR,Fahlman RP,Godbout R

A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly.
Nucleic acids research45(8)4632-4641(2017 May)
Lamichhane R,Hammond JA,Pauszek RF 3rd,Anderson RM,Pedron I,van der Schans E,Williamson JR,Millar DP

FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.
The international journal of biochemistry & cell biology841-13(2017 03)
Akter KA,Mansour MA,Hyodo T,Senga T

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DDX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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