DCK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCK
Entrez Gene ID	1633
Full Name	deoxycytidine kinase
General protein information	Preferred Names deoxycytidine kinase Names deoxycytidine kinase deoxynucleoside kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.3
Summary	Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008].
Disorder MIM:	125450

mRNA and Protein(s)

mRNA	Protein	Name
NM_000788.3	NP_000779.1	deoxycytidine kinase
NM_000788.2	NP_000779.1	deoxycytidine kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DCK	NP_000779.1
	DCK	XP_001093411.2
Danio rerio (zebrafish)	dck	NP_001007057.1
Oryza sativa (rice)	Os05g0430200	NP_001055622.1
Rattus norvegicus (Norway rat)	Dck	NP_077072.1
Xenopus tropicalis (tropical clawed frog)	dck.1	NP_001025532.1
Canis lupus familiaris (dog)	DCK	XP_539307.2
Bos taurus (cattle)	DCK	NP_001029745.1
Gallus gallus (chicken)	DCK	NP_001006451.1
Pan troglodytes (chimpanzee)	DCK	XP_517245.2
Mus musculus (house mouse)	Dck	NP_031858.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of DCK knockdown on proliferation, apoptosis and tumorigenicity in vivo of cervical cancer HeLa cells.
Shang QY, Wu CS, Gao HR
Cancer gene therapy24(9)367-372(2017 Sep)

The Role of Deoxycytidine Kinase (dCK) in Radiation-Induced Cell Death.
Zhong R, Xin R, Chen Z, Liang N, Liu Y, Ma S, Liu X
International journal of molecular sciences17(11)(2016 Nov)

dCK Expression and Gene Polymorphism With Gemcitabine Chemosensitivity in Patients With Pancreatic Ductal Adenocarcinoma: A Strobe-Compliant Observational Study.
Xiong J, Altaf K, Ke N, Wang Y, Tang J, Tan C, Li A, Zhang H, He D, Liu X
Medicine95(10)e2936(2016 Mar)

Prognostic effect of hENT1, dCK and HuR expression by morphological type in periampullary adenocarcinoma, including pancreatic cancer.
Elebro J, Ben Dror L, Heby M, Nodin B, Jirstr?m K, Eberhard J
Acta oncologica (Stockholm, Sweden)55(3)286-96(2016)

Potent Sensitisation of Cancer Cells to Anticancer Drugs by a Quadruple Mutant of the Human Deoxycytidine Kinase.
Coulibaly ST, Rossolillo P, Winter F, Kretzschmar FK, Bray? M, Martin DP, Lener D, Negroni M
PloS one10(10)e0140741(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylated and activated DCK can inhibit radiation-induced cell death including apoptosis and mitotic catastrophe, and promote radiation-induced autophagy through PI3K/Akt/mTOR pathway.
Title: The Role of Deoxycytidine Kinase (dCK) in Radiation-Induced Cell Death.

expression and A9846G AA genotype significantly associated with reduced mortality in pancreatic ductal adenocarcinoma
Title: dCK Expression and Gene Polymorphism With Gemcitabine Chemosensitivity in Patients With Pancreatic Ductal Adenocarcinoma: A Strobe-Compliant Observational Study.

Low deoxycytidine kinase expression is associated with periampullary adenocarcinoma.
Title: Prognostic effect of hENT1, dCK and HuR expression by morphological type in periampullary adenocarcinoma, including pancreatic cancer.

results strongly suggest that (1) the E197K alteration in DCK causes inactivation of DCK, and that (2) loss of the normal E197 allele is the crucial mechanism in acquisition of gemcitabine resistance in the RMKN28 tumor cell line
Title: Acquisition of chemoresistance to gemcitabine is induced by a loss-of-function missense mutation of DCK.

recently described a human deoxycytidine kinase mutant (G12) that sensitizes cancer cell lines to treatment with gemcitabine. Here, starting from the G12 variant, we identified a mutant that triggers even greater sensitisation to gemcitabine than G12.
Title: Potent Sensitisation of Cancer Cells to Anticancer Drugs by a Quadruple Mutant of the Human Deoxycytidine Kinase.

The following DCK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17879	NM_000788.3 Latest version!	Homo sapiens deoxycytidine kinase (DCK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu17879	NM_000788.2	Homo sapiens deoxycytidine kinase (DCK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCK ( NM_000788.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000779.1 Homo sapiens deoxycytidine kinase (DCK), mRNA.

Price & Availability↑

CloneID	OHu17879
Clone ID Related Accession (Same CDS sequence)	NM_000788.3 , NM_000788.2
Accession Version	NM_000788.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	deoxycytidine kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601939.1, CD014016.1 and BC103764.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000788.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103764.1, SRR1660803.117849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000286648.10/ ENSP00000286648.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCACCC CGCCCAAGAG AAGCTGCCCG TCTTTCTCAG CCAGCTCTGA GGGGACCCGC 
ATCAAGAAAA TCTCCATCGA AGGGAACATC GCTGCAGGGA AGTCAACATT TGTGAATATC 
CTTAAACAAT TGTGTGAAGA TTGGGAAGTG GTTCCTGAAC CTGTTGCCAG ATGGTGCAAT 
GTTCAAAGTA CTCAAGATGA ATTTGAGGAA CTTACAATGT CTCAGAAAAA TGGTGGGAAT 
GTTCTTCAGA TGATGTATGA GAAACCTGAA CGATGGTCTT TTACCTTCCA AACATATGCC 
TGTCTCAGTC GAATAAGAGC TCAGCTTGCC TCTCTGAATG GCAAGCTCAA AGATGCAGAG 
AAACCTGTAT TATTTTTTGA ACGATCTGTG TATAGTGACA GGTATATTTT TGCATCTAAT 
TTGTATGAAT CTGAATGCAT GAATGAGACA GAGTGGACAA TTTATCAAGA CTGGCATGAC 
TGGATGAATA ACCAATTTGG CCAAAGCCTT GAATTGGATG GAATCATTTA TCTTCAAGCC 
ACTCCAGAGA CATGCTTACA TAGAATATAT TTACGGGGAA GAAATGAAGA GCAAGGCATT 
CCTCTTGAAT ATTTAGAGAA GCTTCATTAT AAACATGAAA GCTGGCTCCT GCATAGGACA 
CTGAAAACCA ACTTCGATTA TCTTCAAGAG GTGCCTATCT TAACACTGGA TGTTAATGAA 
GACTTTAAAG ACAAATATGA AAGTCTGGTT GAAAAGGTCA AAGAGTTTTT GAGTACTTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000779.1
CDS	188..970
Translation	MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

Target ORF information:

RefSeq Version	NM_000788.3
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxycytidine kinase (DCK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000788.3

ORF Insert Sequence:

ATGGCCACCC CGCCCAAGAG AAGCTGCCCG TCTTTCTCAG CCAGCTCTGA GGGGACCCGC 
ATCAAGAAAA TCTCCATCGA AGGGAACATC GCTGCAGGGA AGTCAACATT TGTGAATATC 
CTTAAACAAT TGTGTGAAGA TTGGGAAGTG GTTCCTGAAC CTGTTGCCAG ATGGTGCAAT 
GTTCAAAGTA CTCAAGATGA ATTTGAGGAA CTTACAATGT CTCAGAAAAA TGGTGGGAAT 
GTTCTTCAGA TGATGTATGA GAAACCTGAA CGATGGTCTT TTACCTTCCA AACATATGCC 
TGTCTCAGTC GAATAAGAGC TCAGCTTGCC TCTCTGAATG GCAAGCTCAA AGATGCAGAG 
AAACCTGTAT TATTTTTTGA ACGATCTGTG TATAGTGACA GGTATATTTT TGCATCTAAT 
TTGTATGAAT CTGAATGCAT GAATGAGACA GAGTGGACAA TTTATCAAGA CTGGCATGAC 
TGGATGAATA ACCAATTTGG CCAAAGCCTT GAATTGGATG GAATCATTTA TCTTCAAGCC 
ACTCCAGAGA CATGCTTACA TAGAATATAT TTACGGGGAA GAAATGAAGA GCAAGGCATT 
CCTCTTGAAT ATTTAGAGAA GCTTCATTAT AAACATGAAA GCTGGCTCCT GCATAGGACA 
CTGAAAACCA ACTTCGATTA TCTTCAAGAG GTGCCTATCT TAACACTGGA TGTTAATGAA 
GACTTTAAAG ACAAATATGA AAGTCTGGTT GAAAAGGTCA AAGAGTTTTT GAGTACTTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCK ( NM_000788.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000779.1 Homo sapiens deoxycytidine kinase (DCK), mRNA.

Price & Availability↑

CloneID	OHu17879
Clone ID Related Accession (Same CDS sequence)	NM_000788.3 , NM_000788.2
Accession Version	NM_000788.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	deoxycytidine kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601939.1, CD014016.1 and BC103764.1. This sequence is a reference standard in the RefSeqGene project. On Jul 29, 2008 this sequence version replaced NM_000788.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.117849.1, SRR1660805.38115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCACCC CGCCCAAGAG AAGCTGCCCG TCTTTCTCAG CCAGCTCTGA GGGGACCCGC 
ATCAAGAAAA TCTCCATCGA AGGGAACATC GCTGCAGGGA AGTCAACATT TGTGAATATC 
CTTAAACAAT TGTGTGAAGA TTGGGAAGTG GTTCCTGAAC CTGTTGCCAG ATGGTGCAAT 
GTTCAAAGTA CTCAAGATGA ATTTGAGGAA CTTACAATGT CTCAGAAAAA TGGTGGGAAT 
GTTCTTCAGA TGATGTATGA GAAACCTGAA CGATGGTCTT TTACCTTCCA AACATATGCC 
TGTCTCAGTC GAATAAGAGC TCAGCTTGCC TCTCTGAATG GCAAGCTCAA AGATGCAGAG 
AAACCTGTAT TATTTTTTGA ACGATCTGTG TATAGTGACA GGTATATTTT TGCATCTAAT 
TTGTATGAAT CTGAATGCAT GAATGAGACA GAGTGGACAA TTTATCAAGA CTGGCATGAC 
TGGATGAATA ACCAATTTGG CCAAAGCCTT GAATTGGATG GAATCATTTA TCTTCAAGCC 
ACTCCAGAGA CATGCTTACA TAGAATATAT TTACGGGGAA GAAATGAAGA GCAAGGCATT 
CCTCTTGAAT ATTTAGAGAA GCTTCATTAT AAACATGAAA GCTGGCTCCT GCATAGGACA 
CTGAAAACCA ACTTCGATTA TCTTCAAGAG GTGCCTATCT TAACACTGGA TGTTAATGAA 
GACTTTAAAG ACAAATATGA AAGTCTGGTT GAAAAGGTCA AAGAGTTTTT GAGTACTTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000779.1
CDS	289..1071
Translation	MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

Target ORF information:

RefSeq Version	NM_000788.2
Organism	Homo sapiens(human)
Definition	Homo sapiens deoxycytidine kinase (DCK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000788.2

ORF Insert Sequence:

ATGGCCACCC CGCCCAAGAG AAGCTGCCCG TCTTTCTCAG CCAGCTCTGA GGGGACCCGC 
ATCAAGAAAA TCTCCATCGA AGGGAACATC GCTGCAGGGA AGTCAACATT TGTGAATATC 
CTTAAACAAT TGTGTGAAGA TTGGGAAGTG GTTCCTGAAC CTGTTGCCAG ATGGTGCAAT 
GTTCAAAGTA CTCAAGATGA ATTTGAGGAA CTTACAATGT CTCAGAAAAA TGGTGGGAAT 
GTTCTTCAGA TGATGTATGA GAAACCTGAA CGATGGTCTT TTACCTTCCA AACATATGCC 
TGTCTCAGTC GAATAAGAGC TCAGCTTGCC TCTCTGAATG GCAAGCTCAA AGATGCAGAG 
AAACCTGTAT TATTTTTTGA ACGATCTGTG TATAGTGACA GGTATATTTT TGCATCTAAT 
TTGTATGAAT CTGAATGCAT GAATGAGACA GAGTGGACAA TTTATCAAGA CTGGCATGAC 
TGGATGAATA ACCAATTTGG CCAAAGCCTT GAATTGGATG GAATCATTTA TCTTCAAGCC 
ACTCCAGAGA CATGCTTACA TAGAATATAT TTACGGGGAA GAAATGAAGA GCAAGGCATT 
CCTCTTGAAT ATTTAGAGAA GCTTCATTAT AAACATGAAA GCTGGCTCCT GCATAGGACA 
CTGAAAACCA ACTTCGATTA TCTTCAAGAG GTGCCTATCT TAACACTGGA TGTTAATGAA 
GACTTTAAAG ACAAATATGA AAGTCTGGTT GAAAAGGTCA AAGAGTTTTT GAGTACTTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of DCK knockdown on proliferation, apoptosis and tumorigenicity in vivo of cervical cancer HeLa cells.
Cancer gene therapy24(9)367-372(2017 Sep)
Shang QY,Wu CS,Gao HR

The Role of Deoxycytidine Kinase (dCK) in Radiation-Induced Cell Death.
International journal of molecular sciences17(11)(2016 Nov)
Zhong R,Xin R,Chen Z,Liang N,Liu Y,Ma S,Liu X

dCK Expression and Gene Polymorphism With Gemcitabine Chemosensitivity in Patients With Pancreatic Ductal Adenocarcinoma: A Strobe-Compliant Observational Study.
Medicine95(10)e2936(2016 Mar)
Xiong J,Altaf K,Ke N,Wang Y,Tang J,Tan C,Li A,Zhang H,He D,Liu X

Prognostic effect of hENT1, dCK and HuR expression by morphological type in periampullary adenocarcinoma, including pancreatic cancer.
Acta oncologica (Stockholm, Sweden)55(3)286-96(2016)
Elebro J,Ben Dror L,Heby M,Nodin B,Jirstr?m K,Eberhard J

Potent Sensitisation of Cancer Cells to Anticancer Drugs by a Quadruple Mutant of the Human Deoxycytidine Kinase.
PloS one10(10)e0140741(2015)
Coulibaly ST,Rossolillo P,Winter F,Kretzschmar FK,Bray? M,Martin DP,Lener D,Negroni M

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DCK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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